Gene Dmel\CG3121

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General Information

Symbol

Dmel\CG3121

Species

D. melanogaster

Name

Annotation symbol

CG3121

Feature type

protein_coding_gene

FlyBase ID

FBgn0034957

Gene Model Status

Current

Stock availability

4 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

60B3-60B3

Sequence location

2R:19,904,944..19,907,418 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG3121 (FBgn0034957). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: microtubule binding. Based on sequence similarity, it is predicted to be involved in the biological process: microtubule-based process. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Radial spokehead-like protein. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult testis. Comments on Affy2 ProbeSet: ProbeSet 1633263_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG3121. Gene sequence location is 2R:19904944..19907418.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60B3-60B3

Limits computationally determined from genome sequence between P{lacW}Phm^k07623&P{lacW}tsr^k05633 and P{EP}EP503

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG3121 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG3121-RA

FBtr0072161

NM_138025

2283

708

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG3121-PA

FBpp0072070

82.1

708

4.23

NP_611869

AAF47136

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Radial spokehead-like protein (IPR006802)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9W1D3

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Dorus et al., 2006, Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult testis.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		0.35
Larval Midgut		0.4
Larval Hindgut		0.6
Larval Malpighian Tubules		2.6
Larval Fat Body		74.3
Larval Salivary Gland		1.4
Larval Trachea		0.9
Larval Carcass		1.025
Adult Head		2.1
Adult Eye		3.1
Adult Brain		1.2
Adult Thoracic-Abdominal Ganglion		1.3
Adult Crop		3.1
Adult Midgut		1.1
Adult Hindgut		0.7
Adult Malpighian Tubules		1.4
Adult Fat Body		1
Adult Salivary Gland		2.4
Adult Heart		1.025
Adult VirginFemale Spermatheca		0.3
Adult InseminatedFemale Spermatheca		0.6
Adult Ovary		0.4
Adult Testis		694.7
Adult Male Accessory Gland		0.9
Adult Carcass		2.3
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		0.35
Larval Midgut		0.4
Larval Hindgut		0.6
Larval Malpighian Tubules		2.6
Larval Fat Body		74.3
Larval Salivary Gland		1.4
Larval Trachea		0.9
Larval Carcass		1.025
Adult Head		2.1
Adult Eye		3.1
Adult Brain		1.2
Adult Thoracic-Abdominal Ganglion		1.3
Adult Crop		3.1
Adult Midgut		1.1
Adult Hindgut		0.7
Adult Malpighian Tubules		1.4
Adult Fat Body		1
Adult Salivary Gland		2.4
Adult Heart		1.025
Adult VirginFemale Spermatheca		0.3
Adult InseminatedFemale Spermatheca		0.6
Adult Ovary		0.4
Adult Testis		(694.7)
Adult Male Accessory Gland		0.9
Adult Carcass		2.3
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		0.35
Larval Midgut		0.4
Larval Hindgut		0.6
Larval Malpighian Tubules		2.6
Larval Fat Body		74.3
Larval Salivary Gland		1.4
Larval Trachea		0.9
Larval Carcass		1.025
Adult Head		2.1
Adult Eye		3.1
Adult Brain		1.2
Adult Thoracic-Abdominal Ganglion		1.3
Adult Crop		3.1
Adult Midgut		1.1
Adult Hindgut		0.7
Adult Malpighian Tubules		1.4
Adult Fat Body		1
Adult Salivary Gland		2.4
Adult Heart		1.025
Adult VirginFemale Spermatheca		0.3
Adult InseminatedFemale Spermatheca		0.6
Adult Ovary		0.4
Adult Testis		694.7
Adult Male Accessory Gland		0.9
Adult Carcass		2.3
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		0.35
Larval Midgut		0.4
Larval Hindgut		0.6
Larval Malpighian Tubules		2.6
Larval Fat Body		74.3
Larval Salivary Gland		1.4
Larval Trachea		0.9
Larval Carcass		1.025
Adult Head		2.1
Adult Eye		3.1
Adult Brain		1.2
Adult Thoracic-Abdominal Ganglion		1.3
Adult Crop		3.1
Adult Midgut		1.1
Adult Hindgut		0.7
Adult Malpighian Tubules		1.4
Adult Fat Body		1
Adult Salivary Gland		2.4
Adult Heart		1.025
Adult VirginFemale Spermatheca		0.3
Adult InseminatedFemale Spermatheca		0.6
Adult Ovary		0.4
Adult Testis		694.7
Adult Male Accessory Gland		0.9
Adult Carcass		2.3
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		0.35
Larval Midgut		0.4
Larval Hindgut		0.6
Larval Malpighian Tubules		2.6
Larval Fat Body		74.3
Larval Salivary Gland		1.4
Larval Trachea		0.9
Larval Carcass		1.025
Adult Head		2.1
Adult Eye		3.1
Adult Brain		1.2
Adult Thoracic-Abdominal Ganglion		1.3
Adult Crop		3.1
Adult Midgut		1.1
Adult Hindgut		0.7
Adult Malpighian Tubules		1.4
Adult Fat Body		1
Adult Salivary Gland		2.4
Adult Heart		1.025
Adult VirginFemale Spermatheca		0.3
Adult InseminatedFemale Spermatheca		0.6
Adult Ovary		0.4
Adult Testis		694.7
Adult Male Accessory Gland		0.9
Adult Carcass		2.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		0.35
Larval Midgut		0.4
Larval Hindgut		0.6
Larval Malpighian Tubules		2.6
Larval Fat Body		74.3
Larval Salivary Gland		1.4
Larval Trachea		0.9
Larval Carcass		1.025
Adult Head		2.1
Adult Eye		3.1
Adult Brain		1.2
Adult Thoracic-Abdominal Ganglion		1.3
Adult Crop		3.1
Adult Midgut		1.1
Adult Hindgut		0.7
Adult Malpighian Tubules		1.4
Adult Fat Body		1
Adult Salivary Gland		2.4
Adult Heart		1.025
Adult VirginFemale Spermatheca		0.3
Adult InseminatedFemale Spermatheca		0.6
Adult Ovary		0.4
Adult Testis		694.7
Adult Male Accessory Gland		0.9
Adult Carcass		2.3
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		0.35
Larval Midgut		0.4
Larval Hindgut		0.6
Larval Malpighian Tubules		2.6
Larval Fat Body		74.3
Larval Salivary Gland		1.4
Larval Trachea		0.9
Larval Carcass		1.025
Adult Head		2.1
Adult Eye		3.1
Adult Brain		1.2
Adult Thoracic-Abdominal Ganglion		1.3
Adult Crop		3.1
Adult Midgut		1.1
Adult Hindgut		0.7
Adult Malpighian Tubules		1.4
Adult Fat Body		1
Adult Salivary Gland		2.4
Adult Heart		1.025
Adult VirginFemale Spermatheca		0.3
Adult InseminatedFemale Spermatheca		0.6
Adult Ovary		0.4
Adult Testis		694.7
Adult Male Accessory Gland		0.9
Adult Carcass		2.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		0.35
Larval Midgut		0.4
Larval Hindgut		0.6
Larval Malpighian Tubules		2.6
Larval Fat Body		74.3
Larval Salivary Gland		1.4
Larval Trachea		0.9
Larval Carcass		1.025
Adult Head		2.1
Adult Eye		3.1
Adult Brain		1.2
Adult Thoracic-Abdominal Ganglion		1.3
Adult Crop		3.1
Adult Midgut		1.1
Adult Hindgut		0.7
Adult Malpighian Tubules		1.4
Adult Fat Body		1
Adult Salivary Gland		2.4
Adult Heart		1.025
Adult VirginFemale Spermatheca		0.3
Adult InseminatedFemale Spermatheca		0.6
Adult Ovary		0.4
Adult Testis		694.7
Adult Male Accessory Gland		0.9
Adult Carcass		2.3
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	2.1
NA	Eye	3.1
NA	Brain	1.2
0.35	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	1.3
NA	Crop	3.1
0.4	Midgut	1.1
0.6	Hindgut	0.7
2.6	Malpighian Tubules	1.4
no informative data	Fat Body	1
1.4	Salivary Gland	2.4
NA	Heart	1.025
0.9	Trachea	NA
NA	VirginFemale Spermatheca	0.3
NA	InseminatedFemale Spermatheca	0.6
NA	Ovary	0.4
NA	Testis	694.7
NA	Male Accessory Gland	0.9
1.025	Carcass	2.3

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		33
central nervous system, larvae L3		0
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		11
fat body, pupae P8		12
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		341
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(33)
central nervous system, larvae L3		0
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		11
fat body, pupae P8		12
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		(341)
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		33
central nervous system, larvae L3		0
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		11
fat body, pupae P8		12
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		(341)
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		33
central nervous system, larvae L3		0
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		11
fat body, pupae P8		12
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		341
accessory gland, mated 4-day male		20
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		33
central nervous system, larvae L3		0
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		11
fat body, pupae P8		12
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		341
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(33)
central nervous system, larvae L3		0
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		11
fat body, pupae P8		12
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		(341)
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		33
central nervous system, larvae L3		0
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		11
fat body, pupae P8		12
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		(341)
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		33
central nervous system, larvae L3		0
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		1
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		0
fat body, white prepupae		11
fat body, pupae P8		12
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		341
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed at stages throughout the pupal period, in adult male stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		0
embryo 18-20hr		0
embryo 20-22hr		0
embryo 22-24hr		0
larva L1		0
larva L2		0
larva L3 12hr old		0
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		8
white prepupae new		7
white prepupae 12hr		16
white prepupae 24hr		25
pupae 2d postWPP		29
pupae 3d postWPP		27
pupae 4d postWPP		22
adult male 01day		36
adult male 05day		47
adult male 30day		44
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		0
embryo 18-20hr		0
embryo 20-22hr		0
embryo 22-24hr		0
larva L1		0
larva L2		0
larva L3 12hr old		0
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		8
white prepupae new		7
white prepupae 12hr		16
white prepupae 24hr		25
pupae 2d postWPP		(29)
pupae 3d postWPP		(27)
pupae 4d postWPP		22
adult male 01day		(36)
adult male 05day		(47)
adult male 30day		(44)
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		0
embryo 18-20hr		0
embryo 20-22hr		0
embryo 22-24hr		0
larva L1		0
larva L2		0
larva L3 12hr old		0
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		8
white prepupae new		7
white prepupae 12hr		16
white prepupae 24hr		25
pupae 2d postWPP		29
pupae 3d postWPP		27
pupae 4d postWPP		22
adult male 01day		36
adult male 05day		47
adult male 30day		44
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		0
embryo 18-20hr		0
embryo 20-22hr		0
embryo 22-24hr		0
larva L1		0
larva L2		0
larva L3 12hr old		0
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		8
white prepupae new		7
white prepupae 12hr		16
white prepupae 24hr		25
pupae 2d postWPP		29
pupae 3d postWPP		27
pupae 4d postWPP		22
adult male 01day		36
adult male 05day		47
adult male 30day		44
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		0
embryo 18-20hr		0
embryo 20-22hr		0
embryo 22-24hr		0
larva L1		0
larva L2		0
larva L3 12hr old		0
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		8
white prepupae new		7
white prepupae 12hr		16
white prepupae 24hr		25
pupae 2d postWPP		29
pupae 3d postWPP		27
pupae 4d postWPP		22
adult male 01day		36
adult male 05day		47
adult male 30day		44
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		0
embryo 18-20hr		0
embryo 20-22hr		0
embryo 22-24hr		0
larva L1		0
larva L2		0
larva L3 12hr old		0
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		8
white prepupae new		7
white prepupae 12hr		16
white prepupae 24hr		25
pupae 2d postWPP		29
pupae 3d postWPP		27
pupae 4d postWPP		22
adult male 01day		(36)
adult male 05day		(47)
adult male 30day		(44)
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		0
embryo 18-20hr		0
embryo 20-22hr		0
embryo 22-24hr		0
larva L1		0
larva L2		0
larva L3 12hr old		0
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		8
white prepupae new		7
white prepupae 12hr		16
white prepupae 24hr		25
pupae 2d postWPP		29
pupae 3d postWPP		27
pupae 4d postWPP		22
adult male 01day		36
adult male 05day		47
adult male 30day		44
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		0
embryo 18-20hr		0
embryo 20-22hr		0
embryo 22-24hr		0
larva L1		0
larva L2		0
larva L3 12hr old		0
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		8
white prepupae new		7
white prepupae 12hr		16
white prepupae 24hr		25
pupae 2d postWPP		29
pupae 3d postWPP		27
pupae 4d postWPP		22
adult male 01day		36
adult male 05day		47
adult male 30day		44
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		1
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		1
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		1
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		1
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		1
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		1
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		1
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		1
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		3
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		3
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		3
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		3
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		3
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		3
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		3
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		3
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0034957

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG3121-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

CG3121^GD10192

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of CG3121	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG3121	Mutagen	Stocks	Known lesion
CG3121^GD10192	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
CG3121^HMS01721	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG3121^KK109291	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes

Aneuploid Aberrations

Disrupted in

Df(2R)Exel6080

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD10192}

P{KK109291}

P{TRiP.HMS01721}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 3 terms )

Molecular Function

CV term

Evidence

References

microtubule binding

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

Biological Process

CV term

Evidence

References

microtubule-based process

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

Cellular Component

CV term

Evidence

References

microtubule associated complex

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG3121 allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0034957

Orthologs

OrthoDB Orthologs (37) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6K3MFW

Dipteran inclusive ortholog search

No orthologs identified

Insect inclusive ortholog search

EOG6P2NXR

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

EOG69KTJ3

Orthologs in Drosophila Species (EOG6K3MFW)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA16095

Drosophila persimilis

Dper\GL11649

Drosophila willistoni

Dwil\GK21932

Drosophila virilis

Dvir\GJ20436

Drosophila mojavensis

Dmoj\GI18866

Drosophila grimshawi

Dgri\GH20165

Orthologs in non-Drosophila Dipterans (None identified)

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (EOG6P2NXR)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB46677

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011716

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH15493

Atta cephalotes

Leafcutter ant

Acep\ACEP17699

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO18825

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL15135

Linepithema humile

Argentine ant

Lhum\LH14147

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB22140

Solenopsis invicta

Red fire ant

Sinv\SINV18460

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI48484

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI062478

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI068413

Bombyx mori

Silkmoth

Bmor\BGIBMGA000820

Pediculus humanus

Human body louse

Phum\PHUM547040

Tribolium castaneum

Red flour beetle

Tcas\TC008922

Orthologs in non-Insect Arthropods (None identified)

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (EOG69KTJ3)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_014801gn

Danio rerio

Zebrafish

Drer\rsph4a

Xenopus tropicalis

Western clawed frog

Xtro\rsph6a

Gallus gallus

Domestic chicken

Ggal\RSPH6A

Mus musculus

House mouse

Mmus\Rsph4a

Mus musculus

House mouse

Mmus\Rsph6a

Rattus norvegicus

Norway rat

Rnor\Rsph4a

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000014788

Homo sapiens

Human

Hsap\RSPH4A

Homo sapiens

Human

Hsap\RSPH6A

Human Orthologs (2)

Gene

OMIM

HGNC

Hsap\RSPH4A

Hsap\RSPH6A

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA16095

Drosophila persimilis

Dper\GL11649

Drosophila willistoni

Dwil\GK21932

Drosophila virilis

Dvir\GJ20436

Drosophila mojavensis

Dmoj\GI18866

Drosophila grimshawi

Dgri\GH20165

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Bloomington

38524

y¹ sc^* v¹; P{TRiP.HMS01721}attP40

VDRC

v106848

P{KK109291}VIE-260B

v25712

w¹¹¹⁸; P{GD10192}v25712

v25713

w¹¹¹⁸; P{GD10192}v25713

Genomic Clones ( 1 )

BACR28M05

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 4 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH13165

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

AT27260

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0034957

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

37831

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG3121 anon-WO0140519.109

(FlyBase, 1996-)

Additional comments

Source for merge of CG3121 anon-WO0140519.109 was sequence comparison (date:051113).

(FlyBase, 1996-)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_138025

RefSeq (Proteins)

•

NP_611869

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

37831

UniProtKB/TrEMBL

•

Q9W1D3

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Radial spokehead-like protein (IPR006802)

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0034957

DRSC - Results from RNAi screens.

•

FBgn0034957

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0034957

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG3121-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0034957

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

37831

modMine - Data generated by the modENCODE project.

•

FBgn0034957

Synonyms & Secondary IDs ( 2 )

Reported As

Symbol Synonym

anon-WO0140519.109

CG3121

(Dorus et al., 2006, Keleman et al., 2009.8.5, Wasbrough et al., 2010, Ni et al., 2010.12.1)

Name Synonym

Secondary FlyBase IDs

FBgn0044775

References ( 15 )

Research paper

Avidor-Reiss et al., 2004, Cell 117(4): 527--539: Decoding cilia function; defining specialized genes required for compartmentalized cilia biogenesis. [FBrf0174448]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Wasbrough et al., 2010, J. Proteomics 73(11):: Supplemental Table 1. Mass spectrometry data. [FBrf0213252]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Dorus et al., 2006, Nat. Genet. 38(12):: Supplementary Table 1. Genes encoding the Drosophila melanogaster sperm proteome. [FBrf0206129]
Kiger et al., 2003, J. Biol. 2(4):: Additional data file 1: The gene identity and primer sequences for dsRNAs used in the RNAi screens. [FBrf0174231]
Goldstein and Gunawardena, 2000, J. Cell Biol. 150(2):: Table S2. Identified cytoskeletal protein coding genes in Drosophila. [FBrf0132100]

Personal communication to FlyBase

Ni et al., 2010.12.1, A genome-scale shRNA resource for transgenic RNAi in Drosophila.: A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0212437]
Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]

Patent

Greenspan, 2001.6.7, World Patent 0140519-A:: Methods for identifying modulators for balance related disorders. [FBrf0141245]