FlyBase Gene Report: Dmel\Naa35

N-TERMINAL ACETYLTRANSFERASE AUXILIARY SUBUNITS -: N-terminal acetyltransferase auxiliary subunits interact with their respective catalytic subunits and are required for N-terminal acetyltransferase activity. (Adapted from PMID:12890471)
N-TERMINAL ACETYLTRANSFERASE C -: The N-terminal acetyltransferase C (NatC) complex catalyzes the acetylation of the N-terminal methionine of polypeptides with Met-Leu-, Met-Phe-, Met-Ile- and Met-Trp- N termini. (Adapted from PMID:19660096, PMID:22405572 and FBrf0234437).

Protein Function (UniProtKB)

Probable auxillary component of a complex displaying alpha (N-terminal) acetyltransferase activity.

(UniProt, Q9W1A2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Naa35 or the JBrowse view of Dmel\Naa35 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.52

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Naa35-RA

FBtr0072280

NM_138047

2539

784

Naa35-RB

FBtr0100398

NM_001014546

2536

783

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Naa35-PA

FBpp0072187

89.2

784

7.31

NP_611891

AAF47171

Naa35-PB

FBpp0099812

89.1

783

7.31

NP_001014546

AAX52681

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

-alpha-acetyltransferase 35, NatC auxiliary subunit

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Naa35 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG4065_1

Linkouts

Gene Ontology (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

peptide alpha-N-acetyltransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN000483166

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

N-terminal peptidyl-methionine acetylation

inferred from electronic annotation with InterPro:IPR007244

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

N-terminal protein amino acid acetylation

inferred from biological aspect of ancestor with PANTHER:PTN000483166

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

cytoplasm

inferred from sequence or structural similarity with UniProtKB:Q6PHQ8

(assigned by UniProt )

(UniProt Curators, 2002-)

NatC complex

inferred from biological aspect of ancestor with PANTHER:PTN000483166

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Naa35 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Naa35

View exonic expression by tissue for Dmel\Naa35

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034982

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0034982

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG4065-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4065

Flygut - An atlas of the Drosophila adult midgut

FBgn0034982

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Naa35

Stages(s) 1-3
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Naa35

Transgenic constructs containing regulatory region of Naa35

Deletions and Duplications ( 1 )

Disrupted in

Df(2R)Exel6081

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Naa35^GD2199

viable, with Scer\GAL4^Mef2.PR

Naa35^GD2199

viable, with Scer\GAL4^pnr-MD237

Naa35^GD2199

viable, with Scer\GAL4^tin.CΔ4

Naa35^GD2199

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\NAA35

14 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Naa35

14 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Naa35

13 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\naa35

7 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\naa35

14 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\natc-1

13 of 15

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\MAK10

7 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\MAK10

11 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\mak10

9 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091902WI )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Naa35

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17930

Drosophila persimilis

Dper\GL10417

Drosophila willistoni

Dwil\GK23196

Drosophila virilis

Dvir\GJ22137

Drosophila mojavensis

Dmoj\GI19262

Drosophila grimshawi

Dgri\GH21912

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502AW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17024970

Glossina morsitans

Tsetse fly

Gmor\GMOY007158

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_01780

Mayetiola destructor

Hessian fly

Mdes\Mdes001497

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007100

Anopheles darlingi

American malaria mosquito

Adar\ADAC006579

Anopheles gambiae

Malaria mosquito

Agam\AGAP005863

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015222

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015224

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ019243

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W039X )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA002625

Danaus plexippus

Monarch butterfly

Dple\DPOGS201479-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015930

Apis florea

Little honeybee

Aflo\3732948356

Apis mellifera

Western honey bee

Amel\GB55333

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP22772

Bombus terrestris

Buff-tailed bumblebee

Bter\15211385

Linepithema humile

Argentine ant

Lhum\LH24018

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001b8c

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG014227

Tribolium castaneum

Red flour beetle

Tcas\TC004679

Pediculus humanus

Human body louse

Phum\PHUM289970

Cimex lectularius

Bed bug

Clec\CLEC011145

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007036

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11277

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X036U )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR015563

Ixodes scapularis

Black-legged tick

Isca\ISCW017265

Stegodyphus mimosarum

African social velvet spider

Smim\KFM79599.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_301118

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G06Y3 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ift52

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Mak10

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000003925

Gallus gallus

Domestic chicken

Ggal\MAK10

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NAA35; N(alpha)-acetyltransferase 35, NatC auxiliary subunit

14 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63444

DroID - A comprehensive database of gene and protein interactions.

FBgn0034982

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37866

MIST (protein-protein) - An integrated Molecular Interaction Database

CG4065

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-106

Cytogenetic map

60B12-60B12

Sequence location

2R:24,135,180..24,138,050 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60B12-60B12

Limits computationally determined from genome sequence between P{EP}EP503 and P{lacW}Nop60B^k05318

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-106

(FlyBase, 2016)

2-110.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Bloomington

61901

y¹ v¹; P{TRiP.HMJ23456}attP40

c07114

v109595

v3614

w¹¹¹⁸; P{GD2199}v3614

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (33)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

FI19334

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034982

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034982

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37866

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Naa35 CG4065

(Rathore et al., 2016)

Source for database merge of

Source for merge of: CG4065 anon- EST:Nelson1

(Ashburner, 2004.6.30)

Additional comments

Other Comments

Origin and Etymology

Discoverer

Etymology

Named "N(alpha)-acetyltransferase 35" after the human ortholog.

(Rathore et al., 2016)

Identification

External Crossreferences and Linkouts ( 37 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37866

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9W1A2

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034982

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034982

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG4065_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4065

Flygut - An atlas of the Drosophila adult midgut

FBgn0034982

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37866

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004679

InterPro - A database of protein families, domains and functional sites

-alpha-acetyltransferase 35, NatC auxiliary subunit

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4065

modMine - A data warehouse for the modENCODE project

FBgn0034982

Linkouts

BioGRID - A database of protein and genetic interactions.

63444

DroID - A comprehensive database of gene and protein interactions.

FBgn0034982

DRSC - Results frm RNAi screens

FBgn0034982

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0034982

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG4065-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0034982

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37866

MIST (protein-protein) - An integrated Molecular Interaction Database

CG4065

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG4065

(Aradska et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Keleman et al., 2009.8.5, Perkins et al., 2009)

Naa35

(Rathore et al., 2016)

anon-EST:Nelson1

Name Synonyms

N(alpha)-acetyltransferase 35

(Rathore et al., 2016)

Secondary FlyBase IDs

FBgn0023100

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (26)

Report Sections

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