FB2020_06, released Dec 22, 2020

Gene: Dmel\Naa35

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General Information
Symbol
Dmel\Naa35
Species
D. melanogaster
Name
N(alpha)-acetyltransferase 35
Annotation Symbol
CG4065
Feature Type
protein_coding_gene
FlyBase ID
FBgn0034982
Gene Model Status
Current
Stock Availability
3 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Key Links
Genomic Location
Cytogenetic map
60B12-60B12
Sequence location
2R:24,135,180..24,138,050 [-]
Recombination map

2-106

RefSeq locus
NT_033778 REGION:24135180..24138050
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in N-terminal peptidyl-methionine acetylation
inferred from electronic annotation with InterPro:IPR007244
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
involved_in N-terminal protein amino acid acetylation
inferred from biological aspect of ancestor with PANTHER:PTN000483166
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from sequence or structural similarity with UniProtKB:Q6PHQ8
(assigned by UniProt )
(UniProt Curators, 2002-)
part_of NatC complex
inferred from biological aspect of ancestor with PANTHER:PTN000483166
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the MAK10 family. (Q9W1A2)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
N-TERMINAL ACETYLTRANSFERASE AUXILIARY SUBUNITS -
N-terminal acetyltransferase auxiliary subunits interact with their respective catalytic subunits and are required for N-terminal acetyltransferase activity. (Adapted from PMID:12890471)
N-TERMINAL ACETYLTRANSFERASE C COMPLEX -
The N-terminal acetyltransferase C (NatC) complex catalyzes the acetylation of the N-terminal methionine of polypeptides with Met-Leu-, Met-Phe-, Met-Ile- and Met-Trp- N termini. (Adapted from PMID:19660096, PMID:22405572 and FBrf0234437).
Protein Function (UniProtKB)
Probable auxillary component of a complex displaying alpha (N-terminal) acetyltransferase activity.
(UniProt, Q9W1A2)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Naa35 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.52

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Naa35-RA
FBtr0072280
NM_138047
2539
784
Naa35-RB
FBtr0100398
NM_001014546
2536
783
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Naa35-PA
FBpp0072187
89.2
784
7.31
NP_611891
AAF47171
Naa35-PB
FBpp0099812
89.1
783
7.31
NP_001014546
AAX52681
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Naa35 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Naa35 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Naa35
Transgenic constructs containing regulatory region of Naa35
Deletions and Duplications ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\NAA35
13 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Naa35
14 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Naa35
12 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\naa35
8 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\naa35
13 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\natc-1
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\MAK10
7 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\MAK10
12 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\mak10
9 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902WI )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00002277
Drosophila simulans
Dsim\GD24970
Drosophila sechellia
Dsec\GM18267
Drosophila erecta
Dere\GG19948
Drosophila yakuba
Dyak\GE11478
Drosophila ananassae
Dana\GF13474
Drosophila ananassae
Dana\GF20517
Drosophila pseudoobscura pseudoobscura
Dpse\GA17930
Drosophila persimilis
Dper\GL10417
Drosophila willistoni
Dwil\GK23196
Drosophila virilis
Dvir\GJ22137
Drosophila mojavensis
Dmoj\GI19262
Drosophila grimshawi
Dgri\GH21912
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502AW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17024970
Glossina morsitans
Tsetse fly
Gmor\GMOY007158
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_01780
Mayetiola destructor
Hessian fly
Mdes\Mdes001497
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007100
Anopheles darlingi
American malaria mosquito
Adar\ADAC006579
Anopheles gambiae
Malaria mosquito
Agam\AGAP005863
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ015222
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ015224
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ019243
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W039X )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA002625
Danaus plexippus
Monarch butterfly
Dple\DPOGS201479-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL015930
Apis florea
Little honeybee
Aflo\3732948356
Apis mellifera
Western honey bee
Amel\GB55333
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22772
Bombus terrestris
Buff-tailed bumblebee
Bter\15211385
Linepithema humile
Argentine ant
Lhum\LH24018
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001b8c
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG014227
Tribolium castaneum
Red flour beetle
Tcas\TC004679
Pediculus humanus
Human body louse
Phum\PHUM289970
Cimex lectularius
Bed bug
Clec\CLEC011145
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI007036
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_11277
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X036U )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR015563
Ixodes scapularis
Black-legged tick
Isca\ISCW017265
Stegodyphus mimosarum
African social velvet spider
Smim\KFM79599.1
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_301118
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G06Y3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Ift52
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mak10
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000003925
Gallus gallus
Domestic chicken
Ggal\MAK10
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    NAA35; N-alpha-acetyltransferase 35, NatC auxiliary subunit
    13 of 15
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Interactions Browser
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2R
        Recombination map

        2-106

        Cytogenetic map
        60B12-60B12
        Sequence location
        2R:24,135,180..24,138,050 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        60B12-60B12
        Limits computationally determined from genome sequence between P{EP}EP503 and P{lacW}Nop60Bk05318
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        2-106

        (FlyBase, 2016)

        2-110.1

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (3)
        Genomic Clones (17)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (33)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: Naa35 CG4065

        (Rathore et al., 2016)
        Source for database merge of

        Source for merge of: CG4065 anon- EST:Nelson1

        (Ashburner, 2004.6.30)
        Additional comments
        Other Comments
        Origin and Etymology
        Discoverer
        Etymology

        Named "N(alpha)-acetyltransferase 35" after the human ortholog.

        (Rathore et al., 2016)
        Identification
        External Crossreferences and Linkouts ( 38 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (4)
        Reported As
        Symbol Synonym
        CG4065
        (Aradska et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Keleman et al., 2009.8.5, Perkins et al., 2009)
        Naa35
        (Rathore et al., 2016)
        anon-EST:Nelson1
        Name Synonyms
        N(alpha)-acetyltransferase 35
        (Rathore et al., 2016)
        Secondary FlyBase IDs
        • FBgn0023100
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (27)