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Gene Dmel\slik

General Information
Symbol	Dmel\slik	Species	D. melanogaster
Name	Sterile20-like kinase	Annotation symbol	CG4527
Feature type	protein_coding_gene	FlyBase ID	FBgn0035001
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	Plkk1, slk
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	60C7-60C8	Sequence location	2R:20,279,752..20,290,203 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Sterile20-like kinase is referred to in FlyBase by the symbol Dmel\slik (CG4527, FBgn0035001). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: positive regulation of peptidyl-threonine phosphorylation; cell proliferation; protein phosphorylation; cytokinesis; regulation of localization. 21 alleles are reported. The phenotypes of these alleles are annotated with: 1st posterior cell; peripodial epithelium; wing. It has 6 annotated transcripts and 6 annotated polypeptides. Protein features are: Polo kinase kinase; Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult eye, adult brain, larval/adult hindgut, adult Malpighian tubules, adult salivary gland, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. Gene sequence location is 2R:20279752..20290203.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 60C7-60C8   Limits computationally determined from genome sequence between P{PZ}bs03267&P{lacW}l(2)k10502k10502 and P{lacW}Nurf-38k16102
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\slik for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Annotated transcripts do not represent all supported alternative splices within 5' UTR. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) slik-RA FBtr0072363  NM_138064   4863   1300 slik-RB FBtr0072364  NM_166669   6319   1703 slik-RD FBtr0072366  NM_206213   6712   1703 slik-RE FBtr0100470  NM_001014549   4642   1342 slik-RF FBtr0290019  NM_001144280   5818   1635 slik-RG FBtr0332925  NM_206214   5305   1365
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein slik-PA   FBpp0072270   147.5   1300   6.54     NP_611908   AAM68311 slik-PB   FBpp0072271   192.1   1703   6.60     NP_726441   AAF47198 slik-PD   FBpp0072273   192.1   1703   6.60     NP_995935   AAS64767 slik-PE   FBpp0099900   152.1   1342   6.39     NP_001014549   AAX52684 slik-PF   FBpp0288458   184.6   1635   6.72     NP_001137752   ACL83205 slik-PG   FBpp0305145   155.1   1365   6.63     NP_995936   AAS64766
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Polo kinase kinase (IPR022165)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA978756   AA990894   AE013599 AAF47198   AE013599 AAM68311   AE013599 AAS64766   AE013599 AAS64767   AE013599 AAX52684   AE013599 ACL83205   AI109900   AI389747   AI405966   AI512610   AI519114   AI520437   AI531946   AI544384   AI546657   AI546693   AJ577475 CAE12059   AW941737   AW943730   AW944669   AY058322 AAL13551   AY075588   AY119617 AAM50271   BF486684   BF488591   BF488778   BF494148   BF496603   BF498212   BF498954   BG633940   BG638874   BG638985   BG640491   BH854550   BI236730   BI485613   BI641203   BT031144   CO177639   CO182976   CO187458   CO264174   CO264391   CO266181   CO269324   CO273236   CO281909   CO281916   CO295860   CO301578   CO307094   CO321542   CO322277   CO323136   CO324533   CO325139   CO325379   CO326031   CO326725   CO327302   CO327734   CO339070   CO344010   CZ466319   CZ468120   CZ470056   CZ470057   EC051529   EC051998   EC054508   EC056449   EC059631   EC060797   EC061259   EC071382   EC073010   EC073791   EC074654   EC080508   EC084047   EC086380   EC088926   EC198465   EC200942   EC211371   EC211786   EC212163   EC232466   EC232858   EC236016   EC241508   EC243072   EC253848   EC254497   EC254557   EC258983   EC259065   EC259807   EL884401   EL884516   EL884640   EL884748   EV596034
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8MLP1 Q9W179 B7YZQ1 Q59E61 Q8MRH6 Q95U41 Q7YZB2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Dorus et al., 2006)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult eye, adult brain, larval/adult hindgut, adult Malpighian tubules, adult salivary gland, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    42 central nervous system, larvae L3    50 central nervous system, pupae P8    32 head, virgin 1-day female    29 head, virgin 4-day female    31 head, virgin 20-day female    29 head, mated 1-day female    31 head, mated 4-day female    27 head, mated 20-day female    27 head, mated 1-day male    46 head, mated 4-day male    46 head, mated 20-day male    45 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    24 digestive system, 4-day adult    31 digestive system, 20-day adult    33 fat body, larvae L3 wandering    19 fat body, white prepupae    25 fat body, pupae P8    16 carcass, larvae L3 wandering    34 carcass, 1-day adult    19 carcass, 4-day adult    20 carcass, 20-day adult    22 ovary, virgin 4-day female    35 ovary, mated 4-day female    34 testis, mated 4-day male    28 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (42) central nervous system, larvae L3  (50) central nervous system, pupae P8  (32) head, virgin 1-day female  (29) head, virgin 4-day female  (31) head, virgin 20-day female  (29) head, mated 1-day female  (31) head, mated 4-day female  (27) head, mated 20-day female  (27) head, mated 1-day male  (46) head, mated 4-day male  (46) head, mated 20-day male  (45) salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    18 digestive system, larvae L3 wandering  (29) digestive system, 1-day adult    24 digestive system, 4-day adult  (31) digestive system, 20-day adult  (33) fat body, larvae L3 wandering    19 fat body, white prepupae    25 fat body, pupae P8    16 carcass, larvae L3 wandering  (34) carcass, 1-day adult    19 carcass, 4-day adult    20 carcass, 20-day adult    22 ovary, virgin 4-day female  (35) ovary, mated 4-day female  (34) testis, mated 4-day male  (28) accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    42 central nervous system, larvae L3    50 central nervous system, pupae P8    32 head, virgin 1-day female    29 head, virgin 4-day female    31 head, virgin 20-day female    29 head, mated 1-day female    31 head, mated 4-day female    27 head, mated 20-day female    27 head, mated 1-day male    46 head, mated 4-day male    46 head, mated 20-day male    45 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    24 digestive system, 4-day adult    31 digestive system, 20-day adult    33 fat body, larvae L3 wandering    19 fat body, white prepupae    25 fat body, pupae P8    16 carcass, larvae L3 wandering    34 carcass, 1-day adult    19 carcass, 4-day adult    20 carcass, 20-day adult    22 ovary, virgin 4-day female    35 ovary, mated 4-day female    34 testis, mated 4-day male    28 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    42 central nervous system, larvae L3    50 central nervous system, pupae P8    32 head, virgin 1-day female    29 head, virgin 4-day female    31 head, virgin 20-day female    29 head, mated 1-day female    31 head, mated 4-day female    27 head, mated 20-day female    27 head, mated 1-day male    46 head, mated 4-day male    46 head, mated 20-day male    45 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    24 digestive system, 4-day adult    31 digestive system, 20-day adult    33 fat body, larvae L3 wandering    19 fat body, white prepupae    25 fat body, pupae P8    16 carcass, larvae L3 wandering    34 carcass, 1-day adult    19 carcass, 4-day adult    20 carcass, 20-day adult    22 ovary, virgin 4-day female    35 ovary, mated 4-day female    34 testis, mated 4-day male    28 accessory gland, mated 4-day male    18 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    42 central nervous system, larvae L3    50 central nervous system, pupae P8    32 head, virgin 1-day female    29 head, virgin 4-day female    31 head, virgin 20-day female    29 head, mated 1-day female    31 head, mated 4-day female    27 head, mated 20-day female    27 head, mated 1-day male    46 head, mated 4-day male    46 head, mated 20-day male    45 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    24 digestive system, 4-day adult    31 digestive system, 20-day adult    33 fat body, larvae L3 wandering    19 fat body, white prepupae    25 fat body, pupae P8    16 carcass, larvae L3 wandering    34 carcass, 1-day adult    19 carcass, 4-day adult    20 carcass, 20-day adult    22 ovary, virgin 4-day female    35 ovary, mated 4-day female    34 testis, mated 4-day male    28 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (42) central nervous system, larvae L3  (50) central nervous system, pupae P8  32 head, virgin 1-day female  29 head, virgin 4-day female  31 head, virgin 20-day female  29 head, mated 1-day female  31 head, mated 4-day female  27 head, mated 20-day female  27 head, mated 1-day male  (46) head, mated 4-day male  (46) head, mated 20-day male  (45) salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    18 digestive system, larvae L3 wandering  29 digestive system, 1-day adult    24 digestive system, 4-day adult  31 digestive system, 20-day adult  (33) fat body, larvae L3 wandering    19 fat body, white prepupae    25 fat body, pupae P8    16 carcass, larvae L3 wandering  (34) carcass, 1-day adult    19 carcass, 4-day adult    20 carcass, 20-day adult    22 ovary, virgin 4-day female  (35) ovary, mated 4-day female  (34) testis, mated 4-day male  28 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    42 central nervous system, larvae L3    50 central nervous system, pupae P8    32 head, virgin 1-day female    29 head, virgin 4-day female    31 head, virgin 20-day female    29 head, mated 1-day female    31 head, mated 4-day female    27 head, mated 20-day female    27 head, mated 1-day male    46 head, mated 4-day male    46 head, mated 20-day male    45 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    24 digestive system, 4-day adult    31 digestive system, 20-day adult    33 fat body, larvae L3 wandering    19 fat body, white prepupae    25 fat body, pupae P8    16 carcass, larvae L3 wandering    34 carcass, 1-day adult    19 carcass, 4-day adult    20 carcass, 20-day adult    22 ovary, virgin 4-day female    35 ovary, mated 4-day female    34 testis, mated 4-day male    28 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    42 central nervous system, larvae L3    50 central nervous system, pupae P8    32 head, virgin 1-day female    29 head, virgin 4-day female    31 head, virgin 20-day female    29 head, mated 1-day female    31 head, mated 4-day female    27 head, mated 20-day female    27 head, mated 1-day male    46 head, mated 4-day male    46 head, mated 20-day male    45 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    24 digestive system, 4-day adult    31 digestive system, 20-day adult    33 fat body, larvae L3 wandering    19 fat body, white prepupae    25 fat body, pupae P8    16 carcass, larvae L3 wandering    34 carcass, 1-day adult    19 carcass, 4-day adult    20 carcass, 20-day adult    22 ovary, virgin 4-day female    35 ovary, mated 4-day female    34 testis, mated 4-day male    28 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    55 embryo 04-06hr    42 embryo 06-08hr    46 embryo 08-10hr    43 embryo 10-12hr    30 embryo 12-14hr    38 embryo 14-16hr    39 embryo 16-18hr    34 embryo 18-20hr    31 embryo 20-22hr    45 embryo 22-24hr    41 larva L1    21 larva L2    20 larva L3 12hr old    16 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    22 larva L3 puffstage 7-9    24 white prepupae new    28 white prepupae 12hr    29 white prepupae 24hr    30 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    18 adult male 01day    22 adult male 05day    26 adult male 30day    25 adult female 01day    28 adult female 05day    34 adult female 30day    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (44) embryo 02-04hr  (55) embryo 04-06hr  (42) embryo 06-08hr  (46) embryo 08-10hr  (43) embryo 10-12hr  (30) embryo 12-14hr  (38) embryo 14-16hr  (39) embryo 16-18hr  (34) embryo 18-20hr  (31) embryo 20-22hr  (45) embryo 22-24hr  (41) larva L1    21 larva L2    20 larva L3 12hr old    16 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    22 larva L3 puffstage 7-9    24 white prepupae new  (28) white prepupae 12hr  (29) white prepupae 24hr  (30) pupae 2d postWPP  (33) pupae 3d postWPP    18 pupae 4d postWPP    18 adult male 01day    22 adult male 05day  26 adult male 30day    25 adult female 01day  (28) adult female 05day  (34) adult female 30day  (34) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    55 embryo 04-06hr    42 embryo 06-08hr    46 embryo 08-10hr    43 embryo 10-12hr    30 embryo 12-14hr    38 embryo 14-16hr    39 embryo 16-18hr    34 embryo 18-20hr    31 embryo 20-22hr    45 embryo 22-24hr    41 larva L1    21 larva L2    20 larva L3 12hr old    16 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    22 larva L3 puffstage 7-9    24 white prepupae new    28 white prepupae 12hr    29 white prepupae 24hr    30 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    18 adult male 01day    22 adult male 05day    26 adult male 30day    25 adult female 01day    28 adult female 05day    34 adult female 30day    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    55 embryo 04-06hr    42 embryo 06-08hr    46 embryo 08-10hr    43 embryo 10-12hr    30 embryo 12-14hr    38 embryo 14-16hr    39 embryo 16-18hr    34 embryo 18-20hr    31 embryo 20-22hr    45 embryo 22-24hr    41 larva L1    21 larva L2    20 larva L3 12hr old    16 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    22 larva L3 puffstage 7-9    24 white prepupae new    28 white prepupae 12hr    29 white prepupae 24hr    30 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    18 adult male 01day    22 adult male 05day    26 adult male 30day    25 adult female 01day    28 adult female 05day    34 adult female 30day    34 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    55 embryo 04-06hr    42 embryo 06-08hr    46 embryo 08-10hr    43 embryo 10-12hr    30 embryo 12-14hr    38 embryo 14-16hr    39 embryo 16-18hr    34 embryo 18-20hr    31 embryo 20-22hr    45 embryo 22-24hr    41 larva L1    21 larva L2    20 larva L3 12hr old    16 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    22 larva L3 puffstage 7-9    24 white prepupae new    28 white prepupae 12hr    29 white prepupae 24hr    30 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    18 adult male 01day    22 adult male 05day    26 adult male 30day    25 adult female 01day    28 adult female 05day    34 adult female 30day    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (44) embryo 02-04hr  (55) embryo 04-06hr  (42) embryo 06-08hr  (46) embryo 08-10hr  (43) embryo 10-12hr  30 embryo 12-14hr  (38) embryo 14-16hr  (39) embryo 16-18hr  (34) embryo 18-20hr  31 embryo 20-22hr  (45) embryo 22-24hr  (41) larva L1    21 larva L2    20 larva L3 12hr old    16 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    22 larva L3 puffstage 7-9    24 white prepupae new  28 white prepupae 12hr  29 white prepupae 24hr  30 pupae 2d postWPP  (33) pupae 3d postWPP    18 pupae 4d postWPP    18 adult male 01day    22 adult male 05day  26 adult male 30day    25 adult female 01day  28 adult female 05day  (34) adult female 30day  (34) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    55 embryo 04-06hr    42 embryo 06-08hr    46 embryo 08-10hr    43 embryo 10-12hr    30 embryo 12-14hr    38 embryo 14-16hr    39 embryo 16-18hr    34 embryo 18-20hr    31 embryo 20-22hr    45 embryo 22-24hr    41 larva L1    21 larva L2    20 larva L3 12hr old    16 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    22 larva L3 puffstage 7-9    24 white prepupae new    28 white prepupae 12hr    29 white prepupae 24hr    30 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    18 adult male 01day    22 adult male 05day    26 adult male 30day    25 adult female 01day    28 adult female 05day    34 adult female 30day    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    55 embryo 04-06hr    42 embryo 06-08hr    46 embryo 08-10hr    43 embryo 10-12hr    30 embryo 12-14hr    38 embryo 14-16hr    39 embryo 16-18hr    34 embryo 18-20hr    31 embryo 20-22hr    45 embryo 22-24hr    41 larva L1    21 larva L2    20 larva L3 12hr old    16 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    22 larva L3 puffstage 7-9    24 white prepupae new    28 white prepupae 12hr    29 white prepupae 24hr    30 pupae 2d postWPP    33 pupae 3d postWPP    18 pupae 4d postWPP    18 adult male 01day    22 adult male 05day    26 adult male 30day    25 adult female 01day    28 adult female 05day    34 adult female 30day    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    46 Schneider line 2 Sg4    28 embryonic 1182-4H    100 embryonic GM2    106 embryonic Kc167    11 embryonic S1    24 embryonic S3    40 leg disc CME L1    54 wing disc CME-W2    38 wing disc ML-DmD8    41 wing disc ML-DmD9    61 wing disc ML-DmD16-c3    126 wing disc ML-DmD21    15 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    44 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    43 tumorous blood cells mbn2    88 ovary fGS/OSS    54 ovary OSC    79 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (46) Schneider line 2 Sg4  (28) embryonic 1182-4H  (100) embryonic GM2  (106) embryonic Kc167    11 embryonic S1    24 embryonic S3  (40) leg disc CME L1  (54) wing disc CME-W2  (38) wing disc ML-DmD8  (41) wing disc ML-DmD9  (61) wing disc ML-DmD16-c3  (126) wing disc ML-DmD21    15 wing disc ML-DmD32  (54) haltere disc ML-DmD17-c3  (47) eye-antennal disc ML-DmD11  (36) antennal disc ML-DmD20-c5  (27) mixed discs ML-DmD4-c1  (44) CNS ML-DmBG1-c1  (49) CNS ML-DmBG2-c2  (43) tumorous blood cells mbn2  (88) ovary fGS/OSS  (54) ovary OSC  (79) ovary OSS  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    46 Schneider line 2 Sg4    28 embryonic 1182-4H    100 embryonic GM2  106 embryonic Kc167    11 embryonic S1    24 embryonic S3    40 leg disc CME L1    54 wing disc CME-W2    38 wing disc ML-DmD8    41 wing disc ML-DmD9    61 wing disc ML-DmD16-c3  (126) wing disc ML-DmD21    15 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    44 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    43 tumorous blood cells mbn2    88 ovary fGS/OSS    54 ovary OSC    79 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    46 Schneider line 2 Sg4    28 embryonic 1182-4H    100 embryonic GM2    106 embryonic Kc167    11 embryonic S1    24 embryonic S3    40 leg disc CME L1    54 wing disc CME-W2    38 wing disc ML-DmD8    41 wing disc ML-DmD9    61 wing disc ML-DmD16-c3    126 wing disc ML-DmD21    15 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    44 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    43 tumorous blood cells mbn2    88 ovary fGS/OSS    54 ovary OSC    79 ovary OSS    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    46 Schneider line 2 Sg4    28 embryonic 1182-4H    100 embryonic GM2    106 embryonic Kc167    11 embryonic S1    24 embryonic S3    40 leg disc CME L1    54 wing disc CME-W2    38 wing disc ML-DmD8    41 wing disc ML-DmD9    61 wing disc ML-DmD16-c3    126 wing disc ML-DmD21    15 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    44 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    43 tumorous blood cells mbn2    88 ovary fGS/OSS    54 ovary OSC    79 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (46) Schneider line 2 Sg4  28 embryonic 1182-4H  (100) embryonic GM2  (106) embryonic Kc167    11 embryonic S1    24 embryonic S3  (40) leg disc CME L1  (54) wing disc CME-W2  (38) wing disc ML-DmD8  (41) wing disc ML-DmD9  (61) wing disc ML-DmD16-c3  (126) wing disc ML-DmD21    15 wing disc ML-DmD32  (54) haltere disc ML-DmD17-c3  (47) eye-antennal disc ML-DmD11  (36) antennal disc ML-DmD20-c5  27 mixed discs ML-DmD4-c1  (44) CNS ML-DmBG1-c1  (49) CNS ML-DmBG2-c2  (43) tumorous blood cells mbn2  (88) ovary fGS/OSS  (54) ovary OSC  (79) ovary OSS  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    46 Schneider line 2 Sg4    28 embryonic 1182-4H    100 embryonic GM2  106 embryonic Kc167    11 embryonic S1    24 embryonic S3    40 leg disc CME L1    54 wing disc CME-W2    38 wing disc ML-DmD8    41 wing disc ML-DmD9    61 wing disc ML-DmD16-c3  126 wing disc ML-DmD21    15 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    44 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    43 tumorous blood cells mbn2    88 ovary fGS/OSS    54 ovary OSC    79 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    46 Schneider line 2 Sg4    28 embryonic 1182-4H    100 embryonic GM2    106 embryonic Kc167    11 embryonic S1    24 embryonic S3    40 leg disc CME L1    54 wing disc CME-W2    38 wing disc ML-DmD8    41 wing disc ML-DmD9    61 wing disc ML-DmD16-c3    126 wing disc ML-DmD21    15 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    44 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    43 tumorous blood cells mbn2    88 ovary fGS/OSS    54 ovary OSC    79 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    21 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    36 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    30 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    21 heat shock, 4-day adult  (27) Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult  (36) Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult  (32) Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult  (33) Ethanol 2.5% 3 hrs, larvae L3  (37) Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (30) Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult  (30) Paraquat 10 mM 48 hrs, 4-day adult  (35) Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    21 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    36 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    30 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    21 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    36 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    30 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    21 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    36 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    30 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    21 heat shock, 4-day adult  27 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult  (36) Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult  32 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult  (33) Ethanol 2.5% 3 hrs, larvae L3  (37) Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  30 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult  30 Paraquat 10 mM 48 hrs, 4-day adult  (35) Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    21 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    36 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    30 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    21 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    36 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    14 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    30 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_01 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035001 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4527-RB;CG4527-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | larval stage | partially | recessive slikKG04837 lethal | pupal stage | recessive slik1 partially lethal - majority die (with slik1) slikKG04837 partially lethal - majority die (with slikKG04837) slik1 viable, with Scer\GAL4pnr-MD237 slikGD11338 Other Phenotypes Allele developmental rate defective slik1 developmental rate defective | recessive slikKG04837 increased cell death (with slik1) slikKG04837 increased cell death (with slikKG04837) slik1 increased cell death | somatic clone slik1 increased cell death | somatic clone (with slik1) slikKG04837 increased cell death | somatic clone (with slikKG04837) slik1 increased cell number, with Scer\GAL4ptc-559.1 slikScer\UAS.cHa increased cell number | cell non-autonomous, with Scer\GAL4ptc-559.1 slikScer\UAS.cHa mitotic cell cycle defective | cell non-autonomous, with Scer\GAL4ptc-559.1 slikScer\UAS.cHa small body, with Scer\GAL4arm.PS, slik1 slikScer\UAS.cHa small body, with Scer\GAL4arm.PS, slikScer\UAS.cHa slik1 small body | larval stage slikKG04837 Phenotype manifest in Allele 1st posterior cell, with Scer\GAL4arm.PS slikScer\UAS.cHa ommatidium & microvillus | somatic clone slik1 peripodial epithelium, with Scer\GAL4ptc-559.1 slikScer\UAS.cHa rhabdomere | somatic clone slik1 wing (with slik1) slikKG04837 wing (with slikKG04837) slik1 wing & epithelial cell slik1 slikKG04837 wing & epithelial cell | ectopic slik1 slikKG04837 wing | somatic clone (with slik1) slikKG04837 wing | somatic clone (with slikKG04837) slik1
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of slik Class Mutagen Stocks Known lesion slikKG04837 loss of function allele P-element activity 2 Yes slik5-SZ-3976 1 -- slikc00253 piggyBac transposase 1 -- slikc02670 piggyBac transposase 1 -- slikc05512 piggyBac transposase 1 -- slikCB-5766-3 1 -- slikMB01795 1 -- slik1 P-element activity 0 Yes slik20348 P-element activity 0 -- slikDG16512 P-element activity 0 -- slikEP687 0 -- slikSH1809 0 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of slik Class Mutagen Stocks Known lesion slikGD11338 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes slikGL00051 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes slikdsRNA.cCa in vitro construct - RNAi 0 Yes slikdsRNA.cHa in vitro construct - RNAi 0 Yes slikkd.Scer\UAS in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes slikkin.kd.Scer\UAS in vitro construct - site directed mutagenesis 0 Yes slikkin.Scer\UAS in vitro construct - deletion 0 Yes slikNIG.4527R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes slikScer\UAS.cHa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel6278 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11338} NA P{NIG.4527R} NA P{TRiP.GL00051} NA P{UAS-slik.H} NA P{UAS-slik.kd} NA P{UAS-slik.kin.kd} NA P{UAS-slik.kin} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}slikEP687 NA P{EPg}slik20348 NA P{GSV1}C125C125 NA miscellaneous insertions P{RS3}slikCB-5766-3 NA P{RS5}slik5-SZ-3976 NA P{SUPor-P}slikKG04837 NA P{wHy}slikDG16512 NA PBac{PB}slikc00253 NA PBac{PB}slikc02670 NA PBac{PB}slikc05512 NA insertion of enhancer trap P{lacW}slikSH1809 No insertion of enhancer trap binary system Mi{ET1}slikMB01795 No
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function
CV term Evidence References
protein serine/threonine kinase activity inferred from direct assay (Hughes and Fehon, 2006)
Biological Process
CV term Evidence References
cell proliferation inferred from mutant phenotype (Hipfner and Cohen, 2003) cytokinesis inferred from mutant phenotype (Echard et al., 2004) positive regulation of peptidyl-threonine phosphorylation inferred from mutant phenotype (Kunda et al., 2008) protein phosphorylation inferred from direct assay (Hughes and Fehon, 2006) regulation of localization inferred from mutant phenotype (Hughes and Fehon, 2006)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) polo kinase kinase activity non-traceable author statement (Tavares, 2003.7.17) receptor signaling protein serine/threonine kinase activity inferred from sequence or structural similarity with EMBL:AF112855 (FlyBase, 1992-) non-traceable author statement (Morrison et al., 2000)
Biological Process
CV term Evidence References
protein phosphorylation non-traceable author statement (Morrison et al., 2000, Tavares, 2003.7.17)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
slik - aru experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions slik allele Gene References slik1 Moe (Hipfner et al., 2004) phl (Hipfner and Cohen, 2003) slikKG04837 phl (Hipfner and Cohen, 2003) slikScer\UAS.cHa phl (Hipfner and Cohen, 2003)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0035001 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37893 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • B7YZQ1
Orthologs
OrthoDB Orthologs (35) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG68D1DQ Dipteran inclusive ortholog search EOG6FTW7V Insect inclusive ortholog search EOG61G208 Arthropod inclusive ortholog search EOG6Z34VT Metazoa inclusive ortholog search EOG6K13F4
Orthologs in Drosophila Species (EOG68D1DQ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\slik     Drosophila simulans   Dsim\GD24961 Y   Drosophila sechellia   Dsec\GM11840 Y   Drosophila erecta   Dere\GG19937 Y   Drosophila yakuba   Dyak\GE11466 Y   Drosophila ananassae   Dana\GF13464 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18234 Y   Drosophila persimilis   Dper\GL20458 Y   Drosophila willistoni   Dwil\GK22086 Y   Drosophila virilis   Dvir\GJ20738 Y   Drosophila mojavensis   Dmoj\GI18341 Y   Drosophila grimshawi   Dgri\GH20904 Y
Orthologs in non-Drosophila Dipterans (EOG6FTW7V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004412   Anopheles gambiae Malaria mosquito  Agam\AGAP010551   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ014503   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ014504
Orthologs in non-Dipteran Insects (EOG61G208)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB50204   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG020539   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH18054   Atta cephalotes Leafcutter ant  Acep\ACEP14222   Camponotus floridanus Florida carpenter ant  Cflo\CFLO21298   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23420   Linepithema humile Argentine ant  Lhum\LH12463   Pogonomyrmex barbatus Red harvester ant  Pbar\PB12078   Solenopsis invicta Red fire ant  Sinv\SINV21081   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002580   Bombyx mori Silkmoth  Bmor\BGIBMGA009558   Bombyx mori Silkmoth  Bmor\BGIBMGA009557   Pediculus humanus Human body louse  Phum\PHUM512860   Tribolium castaneum Red flour beetle  Tcas\TC000382
Orthologs in non-Insect Arthropods (EOG6Z34VT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\COI   Daphnia pulex Water flea  Dpux\JGI_V11_55140   Ixodes scapularis Deer tick  Isca\ISCW014720
Orthologs in non-Arthropod Metazoa (EOG6K13F4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\gck-4   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020190gn
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24961 Drosophila sechellia Dsec\GM11840 Drosophila erecta Dere\GG19937 Drosophila yakuba Dyak\GE11466 Drosophila ananassae Dana\GF13464 Drosophila pseudoobscura pseudoobscura Dpse\GA18234 Drosophila persimilis Dper\GL20458 Drosophila willistoni Dwil\GK22086 Drosophila virilis Dvir\GJ20738 Drosophila mojavensis Dmoj\GI18341 Drosophila grimshawi Dgri\GH20904
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	35179 y1 sc* v1; P{TRiP.GL00051}attP2 14435 y1; P{SUPor-P}slikKG04837/CyO; ry506 23214 y1 w*/Dp(1;Y)y+; Mi{ET1}slikMB01795
Harvard	- PBac{PB}slikc00253 - PBac{PB}slikc02670 - PBac{PB}slikc05512
Kyoto	123806 w1118; P{RS3}slikCB-5766-3 126265 w1118; P{RS5}slik5-SZ-3976 114386 yd2 w1118 P{ey-FLP.N}2; P{neoFRT}42D P{SUPor-P}slikKG04837 /CyO y+
VDRC	v43783 w1118; P{GD11338}v43783 v43784 w1118; P{GD11338}v43784
Genomic Clones ( 1 )
	BACR02A05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 86 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH09291 LD44530 LP09626 SD10926
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT02576 AT10479 AT13443 AT21169 AT24063 AT24419 AT29928 GH20991 GH26131 LD32911 LD34405 RE32837 RE68978 SD03260 SD09841 SD10426 SD11520 SD24420
Other clones	10738 17142 19984 28588 29139 30105 35158 51414 54992 60921 64286 66289 67532 67673 78013 148049 214353.70 214379.66 214417.38 214499.69 214539.66 214539.67 AT12586 CK01.34D.H7 CK01.66B.E12 CK01.122C.H3 CK01.123D.G9 CK01.131B.E7 Dmel_neb_007245_0614_2872 EC24370 EC051529 EC051998 EC054508 EC056449 EK029532 EK053950 EK094130 EK110212 EK137821 EK138936 EK145231 EK159061 EK159104 EK181444 EK185864 EK187809 EK189810 EK194059 EK195661 EK196349 EK211837 EK253541 EP00738 EP03112 EP04519 EP06739 EP07939 EP18518 IP15695 IP15795 IP15895 IP15995 LD38530 LD40603
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0035001 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37893
Antibody Information
	polyclonal (Alves et al., 2006)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Plkk1 CG4527 (FlyBase, 1996-)
Source for database merge of
Additional comments	Source for identity of Plkk1 CG4527 was sequence comparison (date:030717). (FlyBase, 1996-)
Other Comments
	dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells. (Echard et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_138064 NM_166669 NM_206213 NM_206214 NM_001014549 NM_001144280 RefSeq (Proteins) • NP_611908 NP_726441 NP_995935 NP_995936 NP_001014549 NP_001137752 DDBJ / EMBL / GenBank • AAF47198 AAF47198.2 AAL13551 AAM50271 AAM68311 AAM68311.1 AAS64766 AAS64766.1 AAS64767 AAS64767.1 AAX52684 ACL83205 AI109900 AI512610 AI544384 AJ577475 AW941737 AW943730 AW944669 AY058322 AY075588 AY119617 BH854550 CAE12059 CZ466319 CZ468120 CZ470056 CZ470057 Entrez Gene - A searchable database of RefSeq genes. • 37893 UniProtKB/TrEMBL • B7YZQ1 Q7YZB2 Q8MLP1 Q8MRH6 Q9W179 Q59E61 Q95U41
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Polo kinase kinase (IPR022165)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0035001 DRSC - Results from RNAi screens. • FBgn0035001 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035001 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4527-RB;CG4527-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0035001 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37893 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37893 modMine - Data generated by the modENCODE project. • FBgn0035001 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • B7YZQ1
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	CG4527 (Dan et al., 2001, Chen et al., 2004, Orian et al., 2003, Boutanaev et al., 2002, Molnar et al., 2006, Ni et al., 2011.7.19) dPlkk (Alves et al., 2006) DPlkk1 (Tavares, 2003.7.17) dPlkk/Slik (Carreno et al., 2007) Plkk1 (Foley and O'Farrell, 2004, Molnar et al., 2006, Dorus et al., 2006, Dorus et al., 2006, Kunda et al., 2008) PLKK1 (Carreno et al., 2008) Plkk/Slik (Roubinet et al., 2007) slik (Hipfner and Cohen, 2003, Hughes and Fehon, 2006, Junion et al., 2007, Parrish et al., 2009, Suganuma et al., 2010, Hughes et al., 2010, Alves et al., 2006, Japanese National Institute of Genetics, 2012.5.21) Slik (Hughes and Fehon, 2006, Luque and Milan, 2007, Carreno et al., 2008, Kunda et al., 2008, Hughes et al., 2010, Kunda et al., 2012) Slik1 (Jacques et al., 2009) slk (Hipfner et al., 2002) SLK (Morrison et al., 2000) Slk (Garcia et al., 2007)
Name Synonym	dPlkk/Slik threonine kinase (Carreno et al., 2007) polo kinase kinase 1 (Echard et al., 2004) Polo kinase kinase 1 (Tavares, 2003.7.17) Ste20 kinase (Hughes and Fehon, 2006) Sterile20-like kinase (Hipfner et al., 2002)
Secondary FlyBase IDs
References ( 54 )
Generate a list of	All references relating to this gene ( 54 )
List References by type	Research paper  ( 22 ) Supplementary material  ( 7 ) Review  ( 3 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 4 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Conference report  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Kunda et al., 2012, Curr. Biol. 22(3): 231--236 PP1-Mediated Moesin Dephosphorylation Couples Polar Relaxation to Mitotic Exit. [FBrf0217436] Brooks et al., 2011, Genome Res. 21(2): 193--202 Conservation of an RNA regulatory map between Drosophila and mammals. [FBrf0213079] Roubinet et al., 2011, J. Cell Biol. 195(1): 99--112 Molecular networks linked by Moesin drive remodeling of the cell cortex during mitosis. [FBrf0216647] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...