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General Information
Symbol
Dmel\ITP
Species
D. melanogaster
Name
Ion transport peptide
Annotation Symbol
CG13586
Feature Type
FlyBase ID
FBgn0035023
Gene Model Status
Stock Availability
Gene Snapshot
Ion transport peptide (ITP) encodes three identified alternative splice-forms of neuropeptides, one short and two longer forms. The short isoform is produced in several neurosecretory neurons and interneurons in the CNS controling the evening peak of locomotor activity of the fly. [Date last reviewed: 2019-03-07]
Also Known As
DrmITP, DrmITPL2, DrmITPL1
Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:24,537,421..24,558,961 [+]
Recombination map
2-107
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
Summaries
Gene Group (FlyBase)
NEUROPEPTIDES -
Neuropeptides are secreted into the extracellular space where they interact with cell surface receptors (usually G protein coupled receptors). They are extremely diverse, acting as neurotransmitters, neuromodulators, hormones or growth factors. (Adapted from FBrf0211443 and PMID:27813667).
Summary (Interactive Fly)
an endocrine regulator of thirst and excretion, which integrates water homeostasis with feeding - regulates sleep through the photoperiod network - expressed in pars lateralis neurosecretory neurons, three hindgut-innervating neurons in abdominal ganglia, and a stage-specific number of interneurons and peripheral bipolar neurons
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\ITP or the JBrowse view of Dmel\ITP for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0290218
1771
119
FBtr0290219
3646
119
FBtr0300186
2230
108
FBtr0300187
1531
119
FBtr0343483
974
119
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0288657
14.1
119
8.07
FBpp0288658
13.9
119
8.30
FBpp0289423
12.9
108
8.06
FBpp0289424
13.9
119
8.30
FBpp0310091
13.9
119
8.30
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

119 aa isoforms: ITP-PD, ITP-PF, ITP-PG
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ITP using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (8 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
non-traceable author statement
inferred from electronic annotation with InterPro:IPR001166, InterPro:IPR018251
(assigned by InterPro )
non-traceable author statement
Biological Process (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from expression pattern
inferred from mutant phenotype
inferred from expression pattern
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002186303
(assigned by GO_Central )
non-traceable author statement
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR001166, InterPro:IPR018251
(assigned by InterPro )
inferred from sequence model
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
ITP-protein is expressed in adult pacemaker neurons LNd and Pdf negative s-LNV neuron. Labeling in the cell body is detected throughout the circadian light-/dark-cycle while staining at the axon terminals is strongest at Zeitgeber time 5, 8 and 20.
ITP labels the projection of the fifth Pdf-negative s-LNv neuron to the medulla and lamina. The terminals in the lamina are near the retina, in the region of the lamina monopolar cells. In addition, there is more widespread staining of the medulla.
ITP is co-expressed with a dimm reporter and the Kurs-6 enhancer trap in ipc-1 and ipc-2a neurons.
ITP protein is expressed in the 5th PDF negative s-LNV neuron and in one of the neurons of the LNd cluster. This LNd neuron also expresses Scer\GAL4NPF.4.5 (only in males) and Scer\GAL4Mai179.
There are a maximum of 9 (but normally 8) ITP immunoreactive bilateral neuron pairs in the larval and prepupal central nervous system which are found in dorso-mediolateral positions close to the posterior edges of the calyces of the mushroom body. These neurons, referred to as the ipc-1 neurons, project their axons ipsilaterally to terminate posterior to the ring gland, branch within the corpa cardiaca and terminate on the walls of the anterior aorta. Dendrites emerge medially almost at right angles from the axons and extend in a posterioventral direction and, upon reaching the midline, these fibres continue caudally to terminate in the subesophageal ganglion. One fibre on each side continues without branching through the prothoracic gland and terminate in the corpa allatum. In the adults, there are a maximum of 14 ITP immunoreactive neurons in the protocerebrum. The four pairs of adult ipc-1 neurons are located in a posteriodorsal and mediolateral position within the protocerebrum. They project axons to the retrocerebral complex and other putative neurohemal areas in the dorsal sheaths of the neck connective and thoracic parts of the ventral nerve cord. These neurons also give off side branches that innervate median neuropils surrounding the esophageal orifice. The main axon bundle ipsilaterally joins the nervus corporis cardiaci. Some branches of the main axons continue to make terminals at the dorsal surface of the thoracic ganglia or run within lateral stomatogastric nerves along the proventriculus, continue along thoracic parts of the gut and terminate in the vicinity of the salivary glands. Three other groups of protocerebral neurons are ITP immunoreactive: four ipc-2 neurons, which are found dorso-posteriomedially, give rise to short fibres that cross the midline at the level of the protocerebral bridge, with two fibres running dorsally and caudally to intertwine with the ipc-1 neurons; three to four pairs of ipc-3 extending processes through the superior medial and lateral protocerebrum. Their cell bodies are located close to the anterior lateral and anterior dorsal bases of the medulla. At least the most anteriorly and ventrally positioned neuron projects lateral branches into the accessory medulla; one bilateral, faintly staining ITP immunopositive pair of neurons, called the ipc-4, are found in a dorsomedial position with short projections into protocerebral bridge associated neuropils. These neurites extend along the median bundle to areas around the esophageal orifice. In the ventromedial area of the larval and prepupal subesophageal ganglion, a prominent pair of ITP immunoreactive neurons are found that send projections running to the dorsal side and span almost the entire ventral nerve cord. These neurons lose their immunoreactivity during the pupal stage. Located in the eighth abdominal neuromere, three or four pairs of ITP immunoreactive neurons are seen in larvae, prepupae and adults. The axons of these cells extend first into the medial neuropils and then leave the ventral nerve cord through the eighth abdominal nerve and probably innervate the hindgut. Along the lateral body wall, next to the border of abdominal segments A7 and A8, a pair of bipolar peripheral ITP immunopositive neurons can be seen in larvae and prepupae. In adults, this neuron is located on the dorsal perineural side and close to the roots of the dorsal lateral wing and haltere nerves. This neuron sends one branchless axon along the nerves into the periphery and one axon back into the thoracoabdominal ganglion mass where they branch and terminate in the dorsal perineural sheath.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ITP in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ITP
Transgenic constructs containing regulatory region of ITP
Deletions and Duplications ( 0 )
Phenotypes
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (0)
No records found.
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (0)
No records found.
Caenorhabditis elegans (Nematode, roundworm) (3)
3 of 15
Yes
Yes
2 of 15
No
Yes
2 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190HVL )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150EXR )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0IKC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0INZ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0U5S )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-107
    Cytogenetic map
    Sequence location
    2R:24,537,421..24,558,961 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    60D4-60D5
    Limits computationally determined from genome sequence between P{PZ}bs03267&P{lacW}l(2)k10502k10502 and P{lacW}Nurf-38k16102
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (8)
    Genomic Clones (19)
    cDNA Clones (34)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for identity of: itp CG13586
        Source for identity of: ITP itp
        Source for database merge of
        Source for merge of: CG13586 BcDNA:SD05282
        Additional comments
        Source for merge of CG13586 BcDNA:SD05282 was a shared cDNA ( date:020730 ).
        Other Comments
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 47 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        Synonyms and Secondary IDs (15)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (66)