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Gene Dmel\Eps-15

General Information
Symbol	Dmel\Eps-15	Species	D. melanogaster
Name	Epidermal growth factor receptor pathway substrate clone 15	Annotation symbol	CG16932
Feature type	protein_coding_gene	FlyBase ID	FBgn0035060
Gene Model Status	Current	Stock availability	15 publicly available
Also Known As	eps15
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	60E1-60E1	Sequence location	2R:20,655,837..20,662,788 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Epidermal growth factor receptor pathway substrate clone 15 is referred to in FlyBase by the symbol Dmel\Eps-15 (CG16932, FBgn0035060). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: calcium ion binding. There is experimental evidence that it is involved in the biological process: negative regulation of Notch signaling pathway; synaptic vesicle endocytosis; vesicle-mediated transport; regulation of synaptic growth at neuromuscular junction. 22 alleles are reported. The phenotypes of these alleles are annotated with: glial cell; eye disc; macrochaeta; bouton; embryonic/larval neuromuscular junction; socket. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Calcium-binding EF-hand; EF-Hand 1, calcium-binding site; EF-hand-like domain; EPS15 homology (EH). Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval midgut, larval hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval trachea, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1633729_a_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Eps-15. Gene sequence location is 2R:20655837..20662788.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 60E1-60E1   Limits computationally determined from genome sequence between P{lacW}Mmp1k04809 and P{EP}Reg-5EP1065
Experimentally Determined Cytological Location
Cytogenetic map Notes References 60E-60E   Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Eps-15 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.39
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Eps-15-RA FBtr0072435  NM_138121   4421   1253 Eps-15-RB FBtr0072434  NM_166687   4358   1232 Eps-15-RC FBtr0072433  NM_166686   3980   1106 Eps-15-RD FBtr0308684  NM_001259573   3695   792
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Eps-15-PA   FBpp0072340   134.4   1253   4.82     NP_611965   AAF47267 Eps-15-PB   FBpp0072339   132.1   1232   4.81     NP_726482   AAM70793 Eps-15-PC   FBpp0072338   119.6   1106   4.74     NP_726481   AAM70792 Eps-15-PD   FBpp0300890   85.5   792   4.91     NP_001246502   AFH08255
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • EF-Hand 1, calcium-binding site (IPR018247) EPS15 homology (EH) (IPR000261) EF-hand domain (IPR002048) EF-hand-like domain (IPR011992)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA540599   AA803109   AE013599 AAF47267   AE013599 AAM70792   AE013599 AAM70793   AE013599 AFH08255   AI134232   AI260872   AI387663   AI387743   AI388505   AI402625   AI404359   AI406066   AI532767   AI533195   AI533559   AI534517   AI541929   AI542109   AI542956   AJ421624 CAD13484   AQ073649   AW944599   AX093961   AY122260 AAM52772   BF490355   BF491177   BF491474   BF497336   BF499398   BF499466   BF500788   BF504234   BF504615   BG633020   BG633686   BG636846   BG637267   BG639880   BI227959   BI234218   BI236344   BI374921   BI574687   BI574971   BI574995   BI575274   BI575947   BI575956   BI581315   BI583507   BI605891   BI612316   BI613034   BI613907   BI617097   BI621404   BI628669   BI630079   BI635369   BI636059   BI637707   BP542042   BT125713   CK136010   CO154979   CO155666   CO178968   CO189294   CO267751   CO271249   CO298767   CO304251   CO309881   CO329040   CO329789   CO332579   CO339349   CZ471395   CZ471605   CZ471899   EC067834   EC199329   EC207294   EC213327   EC217243   EC221229   EC227818   EC229244   EC231876   EC238001   EC240743   EC245098   EC252787   EC257529   EC262954   EC267444   EL870129   EL871880   EV592406   GH941679   GH943566   GH944428   GH945677   GH946646   GH949747   GH952092   GH953423   GH954934   GH962719   GH964506   GH968794   GH979860   GH981849
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8MMD2 Q8MMD3 Q9W111 Q8WQ61
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference adult stage   lamina neuropil | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R1 | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R2 | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R3 | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R4 | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R5 | restricted   (Hamanaka and Meinertzhagen, 2010) photoreceptor cell R6 | restricted   (Hamanaka and Meinertzhagen, 2010) medulla | restricted   (Hamanaka and Meinertzhagen, 2010)
Additional Descriptive Data
	Eps-15 is expressed in central brain neuropils. It is uniformly high amongst the different neuropils. In cross sections of the lamina, Eps-15 labelling reveals the outline of photoreceptor R1-R6 terminals, and particularly strong staining was seen at the sites facing the interior of the cartridge. Via immunoelectron microscopy, Eps-15 is seen amongst the vesicle pool in the cytoplasm of the photoreceptors, and often close to the capitate projection (invaginations from surrounding epithelial glial and specialised organelles for the endocytosis of vesicle membrane). (Hamanaka and Meinertzhagen, 2010)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
colocalizes_with synaptic vesicle inferred from direct assay (Koh et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval midgut, larval hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval trachea, adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    132.825 Larval Midgut    104.2 Larval Hindgut    116.7 Larval Malpighian Tubules    107.4 Larval Fat Body    103.5 Larval Salivary Gland    87.5 Larval Trachea    184.55 Larval Carcass    81.975 Adult Head    89.6 Adult Eye    216.425 Adult Brain    119.6 Adult Thoracic-Abdominal Ganglion    160.5 Adult Crop    70.8 Adult Midgut    69.6 Adult Hindgut    86.6 Adult Malpighian Tubules    136.4 Adult Fat Body    68.3 Adult Salivary Gland    70.8 Adult Heart    146.6 Adult VirginFemale Spermatheca    83.3 Adult InseminatedFemale Spermatheca    84.3 Adult Ovary    172.3 Adult Testis    212.5 Adult Male Accessory Gland    60 Adult Carcass    89.4 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    132.825 Larval Midgut    104.2 Larval Hindgut    116.7 Larval Malpighian Tubules    107.4 Larval Fat Body    103.5 Larval Salivary Gland    87.5 Larval Trachea    184.55 Larval Carcass    81.975 Adult Head    89.6 Adult Eye    216.425 Adult Brain    119.6 Adult Thoracic-Abdominal Ganglion    160.5 Adult Crop    70.8 Adult Midgut    69.6 Adult Hindgut    86.6 Adult Malpighian Tubules    136.4 Adult Fat Body    68.3 Adult Salivary Gland    70.8 Adult Heart    146.6 Adult VirginFemale Spermatheca    83.3 Adult InseminatedFemale Spermatheca    84.3 Adult Ovary    172.3 Adult Testis    212.5 Adult Male Accessory Gland    60 Adult Carcass    89.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    132.825 Larval Midgut    104.2 Larval Hindgut    116.7 Larval Malpighian Tubules    107.4 Larval Fat Body    103.5 Larval Salivary Gland    87.5 Larval Trachea    184.55 Larval Carcass    81.975 Adult Head    89.6 Adult Eye    216.425 Adult Brain    119.6 Adult Thoracic-Abdominal Ganglion    160.5 Adult Crop    70.8 Adult Midgut    69.6 Adult Hindgut    86.6 Adult Malpighian Tubules    136.4 Adult Fat Body    68.3 Adult Salivary Gland    70.8 Adult Heart    146.6 Adult VirginFemale Spermatheca    83.3 Adult InseminatedFemale Spermatheca    84.3 Adult Ovary    172.3 Adult Testis    212.5 Adult Male Accessory Gland    60 Adult Carcass    89.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    132.825 Larval Midgut    104.2 Larval Hindgut    116.7 Larval Malpighian Tubules    107.4 Larval Fat Body    103.5 Larval Salivary Gland    87.5 Larval Trachea    184.55 Larval Carcass    81.975 Adult Head    89.6 Adult Eye    216.425 Adult Brain    119.6 Adult Thoracic-Abdominal Ganglion    160.5 Adult Crop    70.8 Adult Midgut    69.6 Adult Hindgut    86.6 Adult Malpighian Tubules    136.4 Adult Fat Body    68.3 Adult Salivary Gland    70.8 Adult Heart    146.6 Adult VirginFemale Spermatheca    83.3 Adult InseminatedFemale Spermatheca    84.3 Adult Ovary    172.3 Adult Testis    212.5 Adult Male Accessory Gland    60 Adult Carcass    89.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    132.825 Larval Midgut    104.2 Larval Hindgut    116.7 Larval Malpighian Tubules    107.4 Larval Fat Body    103.5 Larval Salivary Gland    87.5 Larval Trachea    184.55 Larval Carcass    81.975 Adult Head    89.6 Adult Eye    216.425 Adult Brain    119.6 Adult Thoracic-Abdominal Ganglion    160.5 Adult Crop    70.8 Adult Midgut    69.6 Adult Hindgut    86.6 Adult Malpighian Tubules    136.4 Adult Fat Body    68.3 Adult Salivary Gland    70.8 Adult Heart    146.6 Adult VirginFemale Spermatheca    83.3 Adult InseminatedFemale Spermatheca    84.3 Adult Ovary    172.3 Adult Testis    212.5 Adult Male Accessory Gland    60 Adult Carcass    89.4 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    132.825 Larval Midgut    104.2 Larval Hindgut    116.7 Larval Malpighian Tubules    107.4 Larval Fat Body    103.5 Larval Salivary Gland    87.5 Larval Trachea    184.55 Larval Carcass    81.975 Adult Head    89.6 Adult Eye    216.425 Adult Brain    119.6 Adult Thoracic-Abdominal Ganglion    160.5 Adult Crop    70.8 Adult Midgut    69.6 Adult Hindgut    86.6 Adult Malpighian Tubules    136.4 Adult Fat Body    68.3 Adult Salivary Gland    70.8 Adult Heart    146.6 Adult VirginFemale Spermatheca    83.3 Adult InseminatedFemale Spermatheca    84.3 Adult Ovary    172.3 Adult Testis    212.5 Adult Male Accessory Gland    60 Adult Carcass    89.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    132.825 Larval Midgut    104.2 Larval Hindgut    116.7 Larval Malpighian Tubules    107.4 Larval Fat Body    103.5 Larval Salivary Gland    87.5 Larval Trachea    184.55 Larval Carcass    81.975 Adult Head    89.6 Adult Eye    216.425 Adult Brain    119.6 Adult Thoracic-Abdominal Ganglion    160.5 Adult Crop    70.8 Adult Midgut    69.6 Adult Hindgut    86.6 Adult Malpighian Tubules    136.4 Adult Fat Body    68.3 Adult Salivary Gland    70.8 Adult Heart    146.6 Adult VirginFemale Spermatheca    83.3 Adult InseminatedFemale Spermatheca    84.3 Adult Ovary    172.3 Adult Testis    212.5 Adult Male Accessory Gland    60 Adult Carcass    89.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    132.825 Larval Midgut    104.2 Larval Hindgut    116.7 Larval Malpighian Tubules    107.4 Larval Fat Body    103.5 Larval Salivary Gland    87.5 Larval Trachea    184.55 Larval Carcass    81.975 Adult Head    89.6 Adult Eye    216.425 Adult Brain    119.6 Adult Thoracic-Abdominal Ganglion    160.5 Adult Crop    70.8 Adult Midgut    69.6 Adult Hindgut    86.6 Adult Malpighian Tubules    136.4 Adult Fat Body    68.3 Adult Salivary Gland    70.8 Adult Heart    146.6 Adult VirginFemale Spermatheca    83.3 Adult InseminatedFemale Spermatheca    84.3 Adult Ovary    172.3 Adult Testis    212.5 Adult Male Accessory Gland    60 Adult Carcass    89.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    89.6   NA  Eye    216.425   NA  Brain    119.6   132.825  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    160.5   NA  Crop    70.8   104.2  Midgut    69.6   116.7  Hindgut    86.6   107.4  Malpighian Tubules    136.4   103.5  Fat Body    68.3   87.5  Salivary Gland    70.8   NA  Heart    146.6   184.55  Trachea    NA   NA  VirginFemale Spermatheca    83.3   NA  InseminatedFemale Spermatheca    84.3   NA  Ovary    172.3   NA  Testis    212.5   NA  Male Accessory Gland    60   81.975  Carcass    89.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    34 central nervous system, pupae P8    52 head, virgin 1-day female    25 head, virgin 4-day female    22 head, virgin 20-day female    18 head, mated 1-day female    22 head, mated 4-day female    19 head, mated 20-day female    17 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    35 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    14 fat body, white prepupae    21 fat body, pupae P8    33 carcass, larvae L3 wandering    22 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    12 ovary, virgin 4-day female    37 ovary, mated 4-day female    42 testis, mated 4-day male    51 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (41) central nervous system, larvae L3  (34) central nervous system, pupae P8  (52) head, virgin 1-day female    25 head, virgin 4-day female    22 head, virgin 20-day female    18 head, mated 1-day female    22 head, mated 4-day female    19 head, mated 20-day female    17 head, mated 1-day male  (38) head, mated 4-day male  (38) head, mated 20-day male  (35) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    14 fat body, white prepupae    21 fat body, pupae P8  (33) carcass, larvae L3 wandering    22 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    12 ovary, virgin 4-day female  (37) ovary, mated 4-day female  (42) testis, mated 4-day male  (51) accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    34 central nervous system, pupae P8    52 head, virgin 1-day female    25 head, virgin 4-day female    22 head, virgin 20-day female    18 head, mated 1-day female    22 head, mated 4-day female    19 head, mated 20-day female    17 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    35 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    14 fat body, white prepupae    21 fat body, pupae P8    33 carcass, larvae L3 wandering    22 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    12 ovary, virgin 4-day female    37 ovary, mated 4-day female    42 testis, mated 4-day male    51 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    34 central nervous system, pupae P8    52 head, virgin 1-day female    25 head, virgin 4-day female    22 head, virgin 20-day female    18 head, mated 1-day female    22 head, mated 4-day female    19 head, mated 20-day female    17 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    35 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    14 fat body, white prepupae    21 fat body, pupae P8    33 carcass, larvae L3 wandering    22 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    12 ovary, virgin 4-day female    37 ovary, mated 4-day female    42 testis, mated 4-day male    51 accessory gland, mated 4-day male    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    34 central nervous system, pupae P8    52 head, virgin 1-day female    25 head, virgin 4-day female    22 head, virgin 20-day female    18 head, mated 1-day female    22 head, mated 4-day female    19 head, mated 20-day female    17 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    35 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    14 fat body, white prepupae    21 fat body, pupae P8    33 carcass, larvae L3 wandering    22 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    12 ovary, virgin 4-day female    37 ovary, mated 4-day female    42 testis, mated 4-day male    51 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (41) central nervous system, larvae L3  (34) central nervous system, pupae P8  (52) head, virgin 1-day female    25 head, virgin 4-day female    22 head, virgin 20-day female    18 head, mated 1-day female    22 head, mated 4-day female    19 head, mated 20-day female    17 head, mated 1-day male  (38) head, mated 4-day male  (38) head, mated 20-day male  (35) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    14 fat body, white prepupae    21 fat body, pupae P8  (33) carcass, larvae L3 wandering    22 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    12 ovary, virgin 4-day female  (37) ovary, mated 4-day female  (42) testis, mated 4-day male  (51) accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    34 central nervous system, pupae P8    52 head, virgin 1-day female    25 head, virgin 4-day female    22 head, virgin 20-day female    18 head, mated 1-day female    22 head, mated 4-day female    19 head, mated 20-day female    17 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    35 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    14 fat body, white prepupae    21 fat body, pupae P8    33 carcass, larvae L3 wandering    22 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    12 ovary, virgin 4-day female    37 ovary, mated 4-day female    42 testis, mated 4-day male    51 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    34 central nervous system, pupae P8    52 head, virgin 1-day female    25 head, virgin 4-day female    22 head, virgin 20-day female    18 head, mated 1-day female    22 head, mated 4-day female    19 head, mated 20-day female    17 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    35 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    13 digestive system, 20-day adult    14 fat body, larvae L3 wandering    14 fat body, white prepupae    21 fat body, pupae P8    33 carcass, larvae L3 wandering    22 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    12 ovary, virgin 4-day female    37 ovary, mated 4-day female    42 testis, mated 4-day male    51 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    34 embryo 02-04hr    16 embryo 04-06hr    29 embryo 06-08hr    34 embryo 08-10hr    29 embryo 10-12hr    25 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    24 embryo 18-20hr    26 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    21 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    18 white prepupae new    19 white prepupae 12hr    21 white prepupae 24hr    30 pupae 2d postWPP    28 pupae 3d postWPP    23 pupae 4d postWPP    18 adult male 01day    24 adult male 05day    28 adult male 30day    25 adult female 01day    18 adult female 05day    24 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (34) embryo 02-04hr    16 embryo 04-06hr  (29) embryo 06-08hr  (34) embryo 08-10hr  (29) embryo 10-12hr    25 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    24 embryo 18-20hr  26 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    21 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    18 white prepupae new    19 white prepupae 12hr    21 white prepupae 24hr  (30) pupae 2d postWPP  (28) pupae 3d postWPP    23 pupae 4d postWPP    18 adult male 01day    24 adult male 05day  (28) adult male 30day    25 adult female 01day    18 adult female 05day    24 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    34 embryo 02-04hr    16 embryo 04-06hr    29 embryo 06-08hr    34 embryo 08-10hr    29 embryo 10-12hr    25 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    24 embryo 18-20hr    26 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    21 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    18 white prepupae new    19 white prepupae 12hr    21 white prepupae 24hr    30 pupae 2d postWPP    28 pupae 3d postWPP    23 pupae 4d postWPP    18 adult male 01day    24 adult male 05day    28 adult male 30day    25 adult female 01day    18 adult female 05day    24 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    34 embryo 02-04hr    16 embryo 04-06hr    29 embryo 06-08hr    34 embryo 08-10hr    29 embryo 10-12hr    25 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    24 embryo 18-20hr    26 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    21 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    18 white prepupae new    19 white prepupae 12hr    21 white prepupae 24hr    30 pupae 2d postWPP    28 pupae 3d postWPP    23 pupae 4d postWPP    18 adult male 01day    24 adult male 05day    28 adult male 30day    25 adult female 01day    18 adult female 05day    24 adult female 30day    25 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    34 embryo 02-04hr    16 embryo 04-06hr    29 embryo 06-08hr    34 embryo 08-10hr    29 embryo 10-12hr    25 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    24 embryo 18-20hr    26 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    21 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    18 white prepupae new    19 white prepupae 12hr    21 white prepupae 24hr    30 pupae 2d postWPP    28 pupae 3d postWPP    23 pupae 4d postWPP    18 adult male 01day    24 adult male 05day    28 adult male 30day    25 adult female 01day    18 adult female 05day    24 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (34) embryo 02-04hr    16 embryo 04-06hr  29 embryo 06-08hr  (34) embryo 08-10hr  29 embryo 10-12hr    25 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    24 embryo 18-20hr  26 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    21 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    18 white prepupae new    19 white prepupae 12hr    21 white prepupae 24hr  30 pupae 2d postWPP  28 pupae 3d postWPP    23 pupae 4d postWPP    18 adult male 01day    24 adult male 05day  28 adult male 30day    25 adult female 01day    18 adult female 05day    24 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    34 embryo 02-04hr    16 embryo 04-06hr    29 embryo 06-08hr    34 embryo 08-10hr    29 embryo 10-12hr    25 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    24 embryo 18-20hr    26 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    21 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    18 white prepupae new    19 white prepupae 12hr    21 white prepupae 24hr    30 pupae 2d postWPP    28 pupae 3d postWPP    23 pupae 4d postWPP    18 adult male 01day    24 adult male 05day    28 adult male 30day    25 adult female 01day    18 adult female 05day    24 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    34 embryo 02-04hr    16 embryo 04-06hr    29 embryo 06-08hr    34 embryo 08-10hr    29 embryo 10-12hr    25 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    24 embryo 18-20hr    26 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    21 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    18 white prepupae new    19 white prepupae 12hr    21 white prepupae 24hr    30 pupae 2d postWPP    28 pupae 3d postWPP    23 pupae 4d postWPP    18 adult male 01day    24 adult male 05day    28 adult male 30day    25 adult female 01day    18 adult female 05day    24 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    18 embryonic GM2    41 embryonic Kc167    17 embryonic S1    29 embryonic S3    38 leg disc CME L1    49 wing disc CME-W2    36 wing disc ML-DmD8    36 wing disc ML-DmD9    63 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    46 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    52 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    49 CNS ML-DmBG1-c1    70 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    22 ovary fGS/OSS    32 ovary OSC    42 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (34) Schneider line 2 Sg4  (43) embryonic 1182-4H    18 embryonic GM2  (41) embryonic Kc167    17 embryonic S1  (29) embryonic S3  (38) leg disc CME L1  (49) wing disc CME-W2  (36) wing disc ML-DmD8  (36) wing disc ML-DmD9  (63) wing disc ML-DmD16-c3  (59) wing disc ML-DmD21  (46) wing disc ML-DmD32  (52) haltere disc ML-DmD17-c3  (52) eye-antennal disc ML-DmD11  (48) antennal disc ML-DmD20-c5  (47) mixed discs ML-DmD4-c1  (49) CNS ML-DmBG1-c1  (70) CNS ML-DmBG2-c2  (46) tumorous blood cells mbn2    22 ovary fGS/OSS  (32) ovary OSC  (42) ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    18 embryonic GM2    41 embryonic Kc167    17 embryonic S1    29 embryonic S3    38 leg disc CME L1    49 wing disc CME-W2    36 wing disc ML-DmD8    36 wing disc ML-DmD9    63 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    46 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    52 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    49 CNS ML-DmBG1-c1    70 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    22 ovary fGS/OSS    32 ovary OSC    42 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    18 embryonic GM2    41 embryonic Kc167    17 embryonic S1    29 embryonic S3    38 leg disc CME L1    49 wing disc CME-W2    36 wing disc ML-DmD8    36 wing disc ML-DmD9    63 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    46 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    52 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    49 CNS ML-DmBG1-c1    70 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    22 ovary fGS/OSS    32 ovary OSC    42 ovary OSS    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    18 embryonic GM2    41 embryonic Kc167    17 embryonic S1    29 embryonic S3    38 leg disc CME L1    49 wing disc CME-W2    36 wing disc ML-DmD8    36 wing disc ML-DmD9    63 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    46 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    52 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    49 CNS ML-DmBG1-c1    70 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    22 ovary fGS/OSS    32 ovary OSC    42 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (34) Schneider line 2 Sg4  (43) embryonic 1182-4H    18 embryonic GM2  (41) embryonic Kc167    17 embryonic S1  29 embryonic S3  (38) leg disc CME L1  (49) wing disc CME-W2  (36) wing disc ML-DmD8  (36) wing disc ML-DmD9  (63) wing disc ML-DmD16-c3  (59) wing disc ML-DmD21  (46) wing disc ML-DmD32  (52) haltere disc ML-DmD17-c3  (52) eye-antennal disc ML-DmD11  (48) antennal disc ML-DmD20-c5  (47) mixed discs ML-DmD4-c1  (49) CNS ML-DmBG1-c1  (70) CNS ML-DmBG2-c2  (46) tumorous blood cells mbn2    22 ovary fGS/OSS  32 ovary OSC  (42) ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    18 embryonic GM2    41 embryonic Kc167    17 embryonic S1    29 embryonic S3    38 leg disc CME L1    49 wing disc CME-W2    36 wing disc ML-DmD8    36 wing disc ML-DmD9    63 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    46 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    52 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    49 CNS ML-DmBG1-c1    70 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    22 ovary fGS/OSS    32 ovary OSC    42 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    18 embryonic GM2    41 embryonic Kc167    17 embryonic S1    29 embryonic S3    38 leg disc CME L1    49 wing disc CME-W2    36 wing disc ML-DmD8    36 wing disc ML-DmD9    63 wing disc ML-DmD16-c3    59 wing disc ML-DmD21    46 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    52 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    49 CNS ML-DmBG1-c1    70 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    22 ovary fGS/OSS    32 ovary OSC    42 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    18 heat shock, 4-day adult    17 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    18 heat shock, 4-day adult    17 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult  (35) Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3  (31) Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (35) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  (29) Paraquat 10 mM 48 hrs, 4-day adult  (29) Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    18 heat shock, 4-day adult    17 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    18 heat shock, 4-day adult    17 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    18 heat shock, 4-day adult    17 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    18 heat shock, 4-day adult    17 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult  (35) Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3  31 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (35) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  29 Paraquat 10 mM 48 hrs, 4-day adult  29 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    18 heat shock, 4-day adult    17 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    21 cold shock, 4-day adult    18 heat shock, 4-day adult    17 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035060 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG16932-RC
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Eps-15e75 Eps-15unspecified lethal | pharate adult stage Eps-15Δ29 lethal | pharate adult stage (with Eps-15e75) Eps-15Δ29 lethal | pharate adult stage (with Eps-15Δ29) Eps-15e75 lethal | pupal stage Eps-15EP2513 lethal | pupal stage (with Df(2R)Dll-MP) Eps-15EP2513 lethal | pupal stage (with Eps-152) Eps-15EP2513 lethal | pupal stage (with Eps-15EP2513) Eps-152 lethal | recessive, with Scer\GAL4[-]: Eps-15EP2513 lethal | third instar larval stage (with Df(2R)Dll-MP) Eps-15e75 partially lethal - majority die Eps-15EP2513 viable, with Scer\GAL4pnr-MD237 Eps-15GD8784 Sterility Allele fertile Eps-15EP2513 Other Phenotypes Allele neuroanatomy defective, with Scer\GAL4da.G32 Eps-15VDRC.cUa neuroanatomy defective | larval stage (with Df(2R)Dll-MP) Eps-15Δ29 neuroanatomy defective | larval stage (with Eps-15e75) Eps-15Δ29 neuroanatomy defective | larval stage (with Eps-15Δ29) Eps-15e75 neurophysiology defective, with Scer\GAL4[-]: Eps-15EP2513 neurophysiology defective | larval stage (with Df(2R)Dll-MP) Eps-15e75 neurophysiology defective | larval stage (with Eps-15e75) Eps-15Δ29 neurophysiology defective | larval stage (with Eps-15Δ29) Eps-15e75 Phenotype manifest in Allele bouton (with Df(2R)Dll-MP) Eps-15Δ29 bouton (with Eps-15e75) Eps-15Δ29 bouton (with Eps-15Δ29) Eps-15e75 bouton | supernumerary (with Df(2R)Dll-MP) Eps-15Δ29 bouton | supernumerary (with Eps-15e75) Eps-15Δ29 bouton | supernumerary (with Eps-15Δ29) Eps-15e75 embryonic/larval neuromuscular junction Eps-15EP2513 embryonic/larval neuromuscular junction (with Df(2R)Dll-MP) Eps-15e75 Eps-15Δ29 embryonic/larval neuromuscular junction (with Eps-15e75) Eps-15Δ29 embryonic/larval neuromuscular junction (with Eps-15Δ29) Eps-15e75 eo sensory structure | supernumerary, with Scer\GAL4sca-537.4 Eps-15Scer\UAS.cTa eye disc, with Scer\GAL4da.G32 Eps-15VDRC.cUa glial cell, with Scer\GAL4da.G32 Eps-15VDRC.cUa macrochaeta, with Scer\GAL4sca-537.4 Eps-15EP2513 Eps-152 macrochaeta (with Df(2R)Dll-MP), with Scer\GAL4sca-537.4 Eps-15EP2513 macrochaeta (with Eps-152), with Scer\GAL4sca-537.4 Eps-15EP2513 macrochaeta (with Eps-15EP2513), with Scer\GAL4sca-537.4 Eps-152 macrochaeta | supernumerary, with Scer\GAL4sca-537.4 Eps-15Scer\UAS.cTa socket, with Scer\GAL4sca-537.4 Eps-15EP2513 Eps-152 socket (with Df(2R)Dll-MP), with Scer\GAL4sca-537.4 Eps-15EP2513 socket (with Eps-152), with Scer\GAL4sca-537.4 Eps-15EP2513 socket (with Eps-15EP2513), with Scer\GAL4sca-537.4 Eps-152 socket | supernumerary, with Scer\GAL4sca-537.4 Eps-15Scer\UAS.cTa
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of Eps-15 Class Mutagen Stocks Known lesion Eps-15EP2513 P-element activity 2 Yes Eps-15d00130 P-element activity 1 -- Eps-15d00445 P-element activity 1 -- Eps-15e75 Delta2-3 1 Yes Eps-15EP2554 P-element activity 1 -- Eps-15Δ29 FLPase 1 Yes Eps-152 Delta2-3 0 Yes Eps-15d00928 P-element activity 0 -- Eps-15unspecified 0 --
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Eps-15 Class Mutagen Stocks Known lesion Eps-15GD8784 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Eps-15HMS00893 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Eps-15HMS00947 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Eps-15JF03257 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Eps-15KK101943 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Eps-15Scer\UAS.cKa in vitro construct - regulatory fusion 1 Yes Eps-15ΔDPF.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 1 Yes Eps-15+t10.8 in vitro construct - genomic fragment 0 Yes Eps-15+t11.9 in vitro construct - genomic fragment 0 Yes Eps-15+tCH322-150F15 in vitro construct - genomic fragment 0 Yes Eps-15EHmut.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Eps-15Scer\UAS.cTa in vitro construct - regulatory fusion 0 Yes Eps-15VDRC.cUa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC602 (Christensen et al., 2008.9.3) Df(2R)BSC604 (Christensen et al., 2008.9.3) Df(2R)BSC605 (Christensen et al., 2008.9.3) Df(2R)BSC606 (Christensen et al., 2008.9.3) Df(2R)BSC856 (Christensen et al., 2009.7.22) Df(2R)Dll-MP (Koh et al., 2007)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD8784} NA P{KK101943} NA P{TRiP.HMS00893} NA P{TRiP.HMS00947} NA P{TRiP.JF03257} NA P{UAS-Eps-15.EHmut} NA P{UAS-Eps-15.K} NA P{UAS-Eps-15.T} NA P{UAS-Eps-15.ΔDPF} NA characterization construct P{Eps-15+t11.9} NA P{VDRC.Eps-15.U} NA PBac{CH322-150F15} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Eps-15EP2513 NA P{EP}Eps-15EP2554 NA P{GSV1}EP-284EP-284 NA P{XP}Eps-15d00130 NA P{XP}Eps-15d00445 NA P{XP}Eps-15d00928 NA P+PBac{XP3.WH3}Eps-15Δ29 NA miscellaneous insertions P{?EP}Eps-15e75 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
negative regulation of Notch signaling pathway inferred from mutant phenotype (Tang et al., 2005) regulation of synaptic growth at neuromuscular junction inferred from mutant phenotype (Koh et al., 2007) synaptic vesicle endocytosis inferred from mutant phenotype (Koh et al., 2007) vesicle-mediated transport inferred from mutant phenotype (Wairkar et al., 2003)
Cellular Component
CV term Evidence References
colocalizes_with synaptic vesicle inferred from direct assay (Koh et al., 2007)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
calcium ion binding inferred from electronic annotation with InterPro:IPR002048, InterPro:IPR011992 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
neurotransmitter secretion non-traceable author statement (Stimson and Ramaswami, 1999) receptor-mediated endocytosis non-traceable author statement (Stewart, 2002) synaptic vesicle endocytosis traceable author statement (Stimson and Ramaswami, 1999)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Eps-15 - CG11771 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Eps-15 - CG17323 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Eps-15 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63531 DroID - A comprehensive database of gene and protein interactions. • FBgn0035060 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37961
Orthologs
OrthoDB Orthologs (52) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG64MZBD Dipteran inclusive ortholog search EOG61C747 Insect inclusive ortholog search EOG67D8B2 Arthropod inclusive ortholog search EOG68GTJB Metazoa inclusive ortholog search EOG67DP81
Orthologs in Drosophila Species (EOG64MZBD)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Eps-15     Drosophila simulans   Dsim\GD24921 Y   Drosophila sechellia   Dsec\GM11795 Y   Drosophila erecta   Dere\GG19894 Y   Drosophila yakuba   Dyak\GE11419 Y   Drosophila ananassae   Dana\GF14485 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA14224 Y   Drosophila persimilis   Dper\GL17701 Y   Drosophila willistoni   Dwil\GK21653 Y   Drosophila virilis   Dvir\GJ21083 Y   Drosophila mojavensis   Dmoj\GI19514 Y   Drosophila grimshawi   Dgri\GH21541 Y   Drosophila grimshawi   Dgri\GH13466
Orthologs in non-Drosophila Dipterans (EOG61C747)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007950   Anopheles gambiae Malaria mosquito  Agam\AGAP009189
Orthologs in non-Dipteran Insects (EOG67D8B2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB44307   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG036388   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG019743   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH23783   Atta cephalotes Leafcutter ant  Acep\ACEP17141   Atta cephalotes Leafcutter ant  Acep\ACEP17145   Camponotus floridanus Florida carpenter ant  Cflo\CFLO20450   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23489   Linepithema humile Argentine ant  Lhum\LH13250   Linepithema humile Argentine ant  Lhum\LH13248   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24132   Solenopsis invicta Red fire ant  Sinv\SINV13079   Solenopsis invicta Red fire ant  Sinv\SINV13076   Acyrthosiphon pisum Pea aphid  Apim\ACYPI084991   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004565   Bombyx mori Silkmoth  Bmor\BGIBMGA012628   Pediculus humanus Human body louse  Phum\PHUM591970   Tribolium castaneum Red flour beetle  Tcas\TC015931
Orthologs in non-Insect Arthropods (EOG68GTJB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW014300   Ixodes scapularis Deer tick  Isca\ISCW001324
Orthologs in non-Arthropod Metazoa (EOG67DP81)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ehs-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001927gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008123gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024295gn   Danio rerio Zebrafish  Drer\ENSDARG00000067989   Danio rerio Zebrafish  Drer\si:ch73-22b20.2   Danio rerio Zebrafish  Drer\ENSDARG00000042670   Xenopus tropicalis Western clawed frog  Xtro\eps15   Xenopus tropicalis Western clawed frog  Xtro\eps15l1   Gallus gallus Domestic chicken  Ggal\EPS15L1   Gallus gallus Domestic chicken  Ggal\EPS15   Mus musculus House mouse  Mmus\Eps15l1   Mus musculus House mouse  Mmus\Eps15   Rattus norvegicus Norway rat  Rnor\Eps15   Rattus norvegicus Norway rat  Rnor\LOC361120   Homo sapiens Human  Hsap\ENSG00000127527   Homo sapiens Human  Hsap\EPS15
Human Orthologs (2)
Gene OMIM HGNC Hsap\ENSG00000127527     Hsap\EPS15
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24921 Drosophila sechellia Dsec\GM11795 Drosophila erecta Dere\GG19894 Drosophila yakuba Dyak\GE11419 Drosophila ananassae Dana\GF14485 Drosophila pseudoobscura pseudoobscura Dpse\GA14224 Drosophila persimilis Dper\GL17701 Drosophila willistoni Dwil\GK21653 Drosophila virilis Dvir\GJ21083 Drosophila mojavensis Dmoj\GI19514 Drosophila grimshawi Dgri\GH21541
Stocks & Reagents
Stocks Listed in FlyBase ( 15 )
Bloomington	29578 y1 v1; P{TRiP.JF03257}attP2 17068 w1118; P{EP}Eps-15EP2554/CyO 17252 w1118; P{EP}Eps-15EP2513/CyO 24888 w*; P+PBac{XP3.WH3}Eps-15Δ29/SM6a 39671 w*; +/CyO; P{UAS-Eps-15.K}3/TM6B, Tb+ 39672 w*; +/CyO; P{UAS-Eps-15.ΔDPF}3/TM6B, Tb+ 33942 y1 sc* v1; P{TRiP.HMS00893}attP2
Harvard	- P{XP}Eps-15d00130 - P{XP}Eps-15d00445
VDRC	v106678 P{KK101943}VIE-260B v19164 w1118; P{GD8784}v19164 v19165 w1118; P{GD8784}v19165
	More stocks available...
Genomic Clones ( 1 )
	BACR27P17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 100 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD09478
Other clones	MIP26607
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT05553 AT06210 AT11487 AT13948 AT14028 AT15820 AT26807 AT27859 AT28220 GH11630 GH18247 GH19445 GH21878 GH24024 GH26252 LD20337 LP04863 RE25351 RE29880 RE32385 RE62614 RH18688 RH22135 RH26507 RH27527 RH27552 RH30531 RH32365 RH32384 RH40745 RH42101 RH43168 RH47326 RH52740 RH57174 RH59044 RH71896 SD04376 SD04941 SD05429 SD07402 SD08192 SD14651 SD15179 SD16696 SD17625 SD19765 SD19766
Other clones	29669 38579 39966 97115 147678 191240 214385.62 214407.70 277078 524306 525447 CK01.4D.B4 CK01.22B.B1 CK01.23B.A1 CK01.73C.G12 CK01.131D.F10 Dmel_neb_004230_0570_2631 EK043828 EK058058 EK102555 EK151656 EK173824 EK219652 EK244208 EK291924 EK293849 EK303942 EN05135 EN06528 EP01649 FGC008D10 GH18384 GM07026 IP02232 IP03907 RP004107307 RP004129559 RP004149129 RP004183049 RP004217862 RP004226361 RP004227609 RP004240691 RP004244285 RP004249736 RP004264834 RP004450141 RP004503009 RP004521972 SD07894
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0035060 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37961
Antibody Information
	monoclonal (Halder et al., 2011)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Eps-15 CG16932 (Verstreken and Bellen, 2000.9.4)
Source for database merge of	Source for merge of: Eps-15 anon-WO0118547.85 (FlyBase, 1996-)
Additional comments	Source for merge of Eps-15 anon-WO0118547.85 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Area matching Drosophila EST AI260872 (inverted). This EST has sequence similarity to mouse signalling factor U29156. (Davies, 2001.3.15)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_138121 NM_166686 NM_166687 NM_001259573 RefSeq (Proteins) • NP_611965 NP_726481 NP_726482 NP_001246502 DDBJ / EMBL / GenBank • AAF47267 AAM52772 AAM70792 AAM70793 AFH08255 AI260872 AI542956 AJ421624 AQ073649 AW944599 AX093961 AY122260 CAD13484 CZ471395 CZ471605 CZ471899 Entrez Gene - A searchable database of RefSeq genes. • 37961 UniProtKB/TrEMBL • Q8MMD2 Q8MMD3 Q8WQ61 Q9W111
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • EF-Hand 1, calcium-binding site (IPR018247) EPS15 homology (EH) (IPR000261) EF-hand domain (IPR002048) EF-hand-like domain (IPR011992)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63531 DroID - A comprehensive database of gene and protein interactions. • FBgn0035060 DRSC - Results from RNAi screens. • FBgn0035060 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035060 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG16932-RC FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0035060 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37961 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37961 modMine - Data generated by the modENCODE project. • FBgn0035060
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	anon-WO0118547.85   CG16932 (Battersby et al., 2003, Metta et al., 2006, Molnar et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009, Tang et al., 2005, Ni et al., 2010.12.1, Majumdar et al., 2006) EH1 (Tang et al., 2005) eps15 (Venken, 2006, Wairkar et al., 2003, Majumdar et al., 2003, Koh et al., 2007, Vijayakrishnan et al., 2010, Korolchuk et al., 2007, Venken et al., 2009, Kasprowicz et al., 2008) Eps15 (Crosetto, 2005, Wairkar et al., 2003, Miller, 2002, Gonzalez-Gaitan, 2003, Stewart, 2002, Verstreken and Bellen, 2006, Venken et al., 2006, Xie et al., 2012, Yuva-Aydemir et al., 2011, Molnar et al., 2006, Giagtzoglou et al., 2009, Peng et al., 2009, Li et al., 2010, Tang et al., 2005, Wang and Pai, 2011, Franco et al., 2011, Majumdar et al., 2006) eps-15 (Majumdar et al., 2003) EPS-15 (Lloyd et al., 2000, Hamanaka and Meinertzhagen, 2010) Eps-15 (Christensen et al., 2008.9.3, Christensen et al., 2008.9.3, Christensen et al., 2008.9.3, Christensen et al., 2008.9.3, Koh, 2008.12.18, Christensen et al., 2009.7.22, Venken et al., 2009) EPS15 (Tjota et al., 2011) i31 (Battersby et al., 2003)
Name Synonym	Epidermal growth factor receptor pathway substrate clone 15   Eps-15   Eps15 homologue 1 (Tang et al., 2005)
Secondary FlyBase IDs
	FBgn0061582
References ( 61 )
Generate a list of	All references relating to this gene ( 61 )
List References by type	Research paper  ( 23 ) Supplementary material  ( 7 ) Review  ( 6 ) Personal communication to FlyBase  ( 16 ) Abstract  ( 4 ) FlyBase analysis  ( 2 ) Patent  ( 1 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 7 )
	Krauchunas et al., 2012, Dev. Biol. 370(1): 125--134 Protein phosphorylation changes reveal new candidates in the regulation of egg activation and early embryogenesis in D. melanogaster. [FBrf0219342] Xie et al., 2012, Dev. Biol. 363(2): 399--412 Drosophila Epsin's role in Notch ligand cells requires three Epsin protein functions: The lipid binding function of the ENTH domain, a single Ubiquitin interaction motif, and a subset of the C-terminal protein binding modules. [FBrf0217494] Franco et al., 2011, Mol. Cell. Proteomics 10(5): M110.002188 A Novel Strategy to Isolate Ubiquitin Conjugates Reveals Wide Role for Ubiquitination during Neural Development. [FBrf0213919] Halder et al., 2011, PLoS ONE 6(12): e29352 Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain. [FBrf0217036] Tjota et al., 2011, J. Cell Sci. 124(17): 2914--2926 Annexin B9 binds to {beta}H-spectrin and is required for multivesicular body function in Drosophila. [FBrf0215049] Wang and Pai, 2011, PLoS ONE 6(2): e17097 D-Cbl Binding to Drk Leads to Dose-Dependent Down-Regulation of EGFR Signaling and Increases Receptor-Ligand Endocytosis. [FBrf0213073] Yuva-Aydemir et al., 2011, J. Neurosci. 31(19): 7005--7015 Spinster Controls Dpp Signaling during Glial Migration in the Drosophila Eye. [FBrf0213705] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...