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Gene Dmel\wac

General Information
Symbol	Dmel\wac	Species	D. melanogaster
Name	wee Augmin	Annotation symbol	CG13879
Feature type	protein_coding_gene	FlyBase ID	FBgn0035120
Gene Model Status	Current	Stock availability	2 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	61B3-61B3	Sequence location	3L:258,176..259,097 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene wee Augmin is referred to in FlyBase by the symbol Dmel\wac (CG13879, FBgn0035120). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; microtubule binding. There is experimental evidence that it is involved in the biological process: female meiosis; mitotic spindle organization; cytoplasmic microtubule organization; spindle assembly; cell division. 9 alleles are reported. The phenotypes of these alleles are annotated with: eye; macrochaeta; adult cuticle; spindle. It has 3 annotated transcripts and 3 annotated polypeptides. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval central nervous system, larval trachea, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1626918_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of wac. Gene sequence location is 3L:258176..259097.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 61B3-61B3   Limits computationally determined from genome sequence between P{PZ}trh10512 and P{EP}EP3104EP3104
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\wac for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) wac-RB FBtr0289972  NM_138176   634   163 wac-RC FBtr0300711  NM_001169835   633   150 wac-RD FBtr0332106  NM_001274265   808   163
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein wac-PB   FBpp0288410   19.1   163   4.92     NP_612020   AAF47365 wac-PC   FBpp0289935   17.5   150   5.06     NP_001163306   ACZ94578 wac-PD   FBpp0304416   19.1   163   4.92     NP_001261194   AGB93889
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF47365   AE014296 ACZ94578   AE014296 AGB93889   BT032956   BT032962   BT032970   CO322676   EC200598   EW712752
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9W0S8 E1JHV2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
colocalizes_with HAUS complex inferred from direct assay (Guruharsha et al., 2011) microtubule associated complex inferred from direct assay (Hughes et al., 2008) protein complex inferred from direct assay (Meireles et al., 2009) inferred from genetic interaction with dgt2 (Meireles et al., 2009) spindle inferred from direct assay (Meireles et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval central nervous system, larval trachea, adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    343.7 Larval Midgut    32.1 Larval Hindgut    56.8 Larval Malpighian Tubules    35.8 Larval Fat Body    57.7 Larval Salivary Gland    62 Larval Trachea    166.2 Larval Carcass    73.05 Adult Head    75.8 Adult Eye    330.525 Adult Brain    65.4 Adult Thoracic-Abdominal Ganglion    60 Adult Crop    37.5 Adult Midgut    25.9 Adult Hindgut    36.1 Adult Malpighian Tubules    9.5 Adult Fat Body    39 Adult Salivary Gland    37.9 Adult Heart    54.625 Adult VirginFemale Spermatheca    13 Adult InseminatedFemale Spermatheca    11.3 Adult Ovary    265.5 Adult Testis    185.1 Adult Male Accessory Gland    26 Adult Carcass    32.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    343.7 Larval Midgut    32.1 Larval Hindgut    56.8 Larval Malpighian Tubules    35.8 Larval Fat Body    57.7 Larval Salivary Gland    62 Larval Trachea    166.2 Larval Carcass    73.05 Adult Head    75.8 Adult Eye    330.525 Adult Brain    65.4 Adult Thoracic-Abdominal Ganglion    60 Adult Crop    37.5 Adult Midgut    25.9 Adult Hindgut    36.1 Adult Malpighian Tubules    9.5 Adult Fat Body    39 Adult Salivary Gland    37.9 Adult Heart    54.625 Adult VirginFemale Spermatheca    13 Adult InseminatedFemale Spermatheca    11.3 Adult Ovary    265.5 Adult Testis    185.1 Adult Male Accessory Gland    26 Adult Carcass    32.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    343.7 Larval Midgut    32.1 Larval Hindgut    56.8 Larval Malpighian Tubules    35.8 Larval Fat Body    57.7 Larval Salivary Gland    62 Larval Trachea    166.2 Larval Carcass    73.05 Adult Head    75.8 Adult Eye    330.525 Adult Brain    65.4 Adult Thoracic-Abdominal Ganglion    60 Adult Crop    37.5 Adult Midgut    25.9 Adult Hindgut    36.1 Adult Malpighian Tubules    9.5 Adult Fat Body    39 Adult Salivary Gland    37.9 Adult Heart    54.625 Adult VirginFemale Spermatheca    13 Adult InseminatedFemale Spermatheca    11.3 Adult Ovary    265.5 Adult Testis    185.1 Adult Male Accessory Gland    26 Adult Carcass    32.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    343.7 Larval Midgut    32.1 Larval Hindgut    56.8 Larval Malpighian Tubules    35.8 Larval Fat Body    57.7 Larval Salivary Gland    62 Larval Trachea    166.2 Larval Carcass    73.05 Adult Head    75.8 Adult Eye    330.525 Adult Brain    65.4 Adult Thoracic-Abdominal Ganglion    60 Adult Crop    37.5 Adult Midgut    25.9 Adult Hindgut    36.1 Adult Malpighian Tubules    9.5 Adult Fat Body    39 Adult Salivary Gland    37.9 Adult Heart    54.625 Adult VirginFemale Spermatheca    13 Adult InseminatedFemale Spermatheca    11.3 Adult Ovary    265.5 Adult Testis    185.1 Adult Male Accessory Gland    26 Adult Carcass    32.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    343.7 Larval Midgut    32.1 Larval Hindgut    56.8 Larval Malpighian Tubules    35.8 Larval Fat Body    57.7 Larval Salivary Gland    62 Larval Trachea    166.2 Larval Carcass    73.05 Adult Head    75.8 Adult Eye    330.525 Adult Brain    65.4 Adult Thoracic-Abdominal Ganglion    60 Adult Crop    37.5 Adult Midgut    25.9 Adult Hindgut    36.1 Adult Malpighian Tubules    9.5 Adult Fat Body    39 Adult Salivary Gland    37.9 Adult Heart    54.625 Adult VirginFemale Spermatheca    13 Adult InseminatedFemale Spermatheca    11.3 Adult Ovary    265.5 Adult Testis    185.1 Adult Male Accessory Gland    26 Adult Carcass    32.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    343.7 Larval Midgut    32.1 Larval Hindgut    56.8 Larval Malpighian Tubules    35.8 Larval Fat Body    57.7 Larval Salivary Gland    62 Larval Trachea    166.2 Larval Carcass    73.05 Adult Head    75.8 Adult Eye    330.525 Adult Brain    65.4 Adult Thoracic-Abdominal Ganglion    60 Adult Crop    37.5 Adult Midgut    25.9 Adult Hindgut    36.1 Adult Malpighian Tubules    9.5 Adult Fat Body    39 Adult Salivary Gland    37.9 Adult Heart    54.625 Adult VirginFemale Spermatheca    13 Adult InseminatedFemale Spermatheca    11.3 Adult Ovary    265.5 Adult Testis    185.1 Adult Male Accessory Gland    26 Adult Carcass    32.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    343.7 Larval Midgut    32.1 Larval Hindgut    56.8 Larval Malpighian Tubules    35.8 Larval Fat Body    57.7 Larval Salivary Gland    62 Larval Trachea    166.2 Larval Carcass    73.05 Adult Head    75.8 Adult Eye    330.525 Adult Brain    65.4 Adult Thoracic-Abdominal Ganglion    60 Adult Crop    37.5 Adult Midgut    25.9 Adult Hindgut    36.1 Adult Malpighian Tubules    9.5 Adult Fat Body    39 Adult Salivary Gland    37.9 Adult Heart    54.625 Adult VirginFemale Spermatheca    13 Adult InseminatedFemale Spermatheca    11.3 Adult Ovary    265.5 Adult Testis    185.1 Adult Male Accessory Gland    26 Adult Carcass    32.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    343.7 Larval Midgut    32.1 Larval Hindgut    56.8 Larval Malpighian Tubules    35.8 Larval Fat Body    57.7 Larval Salivary Gland    62 Larval Trachea    166.2 Larval Carcass    73.05 Adult Head    75.8 Adult Eye    330.525 Adult Brain    65.4 Adult Thoracic-Abdominal Ganglion    60 Adult Crop    37.5 Adult Midgut    25.9 Adult Hindgut    36.1 Adult Malpighian Tubules    9.5 Adult Fat Body    39 Adult Salivary Gland    37.9 Adult Heart    54.625 Adult VirginFemale Spermatheca    13 Adult InseminatedFemale Spermatheca    11.3 Adult Ovary    265.5 Adult Testis    185.1 Adult Male Accessory Gland    26 Adult Carcass    32.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    75.8   NA  Eye    330.525   NA  Brain    65.4   343.7  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    60   NA  Crop    37.5   32.1  Midgut    25.9   56.8  Hindgut    36.1   35.8  Malpighian Tubules    9.5   57.7  Fat Body    39   62  Salivary Gland    37.9   NA  Heart    54.625   166.2  Trachea    NA   NA  VirginFemale Spermatheca    13   NA  InseminatedFemale Spermatheca    11.3   NA  Ovary    265.5   NA  Testis    185.1   NA  Male Accessory Gland    26   73.05  Carcass    32.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    15 central nervous system, pupae P8    4 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    5 head, mated 1-day female    9 head, mated 4-day female    6 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    11 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    25 ovary, mated 4-day female    16 testis, mated 4-day male    20 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    15 central nervous system, pupae P8    4 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    5 head, mated 1-day female    9 head, mated 4-day female    6 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    11 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    25 ovary, mated 4-day female    16 testis, mated 4-day male    20 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    15 central nervous system, pupae P8    4 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    5 head, mated 1-day female    9 head, mated 4-day female    6 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    11 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    25 ovary, mated 4-day female    16 testis, mated 4-day male    20 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    15 central nervous system, pupae P8    4 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    5 head, mated 1-day female    9 head, mated 4-day female    6 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    11 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    25 ovary, mated 4-day female    16 testis, mated 4-day male    20 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    15 central nervous system, pupae P8    4 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    5 head, mated 1-day female    9 head, mated 4-day female    6 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    11 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    25 ovary, mated 4-day female    16 testis, mated 4-day male    20 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    15 central nervous system, pupae P8    4 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    5 head, mated 1-day female    9 head, mated 4-day female    6 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    11 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    25 ovary, mated 4-day female    16 testis, mated 4-day male    20 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    15 central nervous system, pupae P8    4 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    5 head, mated 1-day female    9 head, mated 4-day female    6 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    11 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    25 ovary, mated 4-day female    16 testis, mated 4-day male    20 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    15 central nervous system, pupae P8    4 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    5 head, mated 1-day female    9 head, mated 4-day female    6 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    11 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    25 ovary, mated 4-day female    16 testis, mated 4-day male    20 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    37 embryo 04-06hr    50 embryo 06-08hr    43 embryo 08-10hr    34 embryo 10-12hr    29 embryo 12-14hr    15 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    24 pupae 2d postWPP    10 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    11 adult male 30day    11 adult female 01day    14 adult female 05day    32 adult female 30day    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (50) embryo 02-04hr  (37) embryo 04-06hr  (50) embryo 06-08hr  (43) embryo 08-10hr  (34) embryo 10-12hr  (29) embryo 12-14hr    15 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    24 pupae 2d postWPP    10 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    11 adult male 30day    11 adult female 01day    14 adult female 05day  (32) adult female 30day  (31) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    37 embryo 04-06hr    50 embryo 06-08hr    43 embryo 08-10hr    34 embryo 10-12hr    29 embryo 12-14hr    15 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    24 pupae 2d postWPP    10 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    11 adult male 30day    11 adult female 01day    14 adult female 05day    32 adult female 30day    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    37 embryo 04-06hr    50 embryo 06-08hr    43 embryo 08-10hr    34 embryo 10-12hr    29 embryo 12-14hr    15 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    24 pupae 2d postWPP    10 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    11 adult male 30day    11 adult female 01day    14 adult female 05day    32 adult female 30day    31 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    37 embryo 04-06hr    50 embryo 06-08hr    43 embryo 08-10hr    34 embryo 10-12hr    29 embryo 12-14hr    15 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    24 pupae 2d postWPP    10 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    11 adult male 30day    11 adult female 01day    14 adult female 05day    32 adult female 30day    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (50) embryo 02-04hr  (37) embryo 04-06hr  (50) embryo 06-08hr  (43) embryo 08-10hr  (34) embryo 10-12hr  29 embryo 12-14hr    15 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    24 pupae 2d postWPP    10 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    11 adult male 30day    11 adult female 01day    14 adult female 05day  32 adult female 30day  31 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    37 embryo 04-06hr    50 embryo 06-08hr    43 embryo 08-10hr    34 embryo 10-12hr    29 embryo 12-14hr    15 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    24 pupae 2d postWPP    10 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    11 adult male 30day    11 adult female 01day    14 adult female 05day    32 adult female 30day    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    37 embryo 04-06hr    50 embryo 06-08hr    43 embryo 08-10hr    34 embryo 10-12hr    29 embryo 12-14hr    15 embryo 14-16hr    5 embryo 16-18hr    4 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    24 pupae 2d postWPP    10 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    10 adult male 05day    11 adult male 30day    11 adult female 01day    14 adult female 05day    32 adult female 30day    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    48 embryonic 1182-4H    30 embryonic GM2    19 embryonic Kc167    26 embryonic S1    38 embryonic S3    45 leg disc CME L1    33 wing disc CME-W2    45 wing disc ML-DmD8    35 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    54 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    67 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    12 ovary OSC    28 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (70) Schneider line 2 Sg4  (48) embryonic 1182-4H  (30) embryonic GM2    19 embryonic Kc167  26 embryonic S1  (38) embryonic S3  (45) leg disc CME L1  (33) wing disc CME-W2  (45) wing disc ML-DmD8  (35) wing disc ML-DmD9    11 wing disc ML-DmD16-c3    25 wing disc ML-DmD21  (54) wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11  (27) antennal disc ML-DmD20-c5  (67) mixed discs ML-DmD4-c1  (35) CNS ML-DmBG1-c1  (37) CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    12 ovary OSC  (28) ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    48 embryonic 1182-4H    30 embryonic GM2    19 embryonic Kc167    26 embryonic S1    38 embryonic S3    45 leg disc CME L1    33 wing disc CME-W2    45 wing disc ML-DmD8    35 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    54 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    67 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    12 ovary OSC    28 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    48 embryonic 1182-4H    30 embryonic GM2    19 embryonic Kc167    26 embryonic S1    38 embryonic S3    45 leg disc CME L1    33 wing disc CME-W2    45 wing disc ML-DmD8    35 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    54 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    67 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    12 ovary OSC    28 ovary OSS    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    48 embryonic 1182-4H    30 embryonic GM2    19 embryonic Kc167    26 embryonic S1    38 embryonic S3    45 leg disc CME L1    33 wing disc CME-W2    45 wing disc ML-DmD8    35 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    54 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    67 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    12 ovary OSC    28 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (70) Schneider line 2 Sg4  (48) embryonic 1182-4H  30 embryonic GM2    19 embryonic Kc167  26 embryonic S1  (38) embryonic S3  (45) leg disc CME L1  (33) wing disc CME-W2  (45) wing disc ML-DmD8  (35) wing disc ML-DmD9    11 wing disc ML-DmD16-c3    25 wing disc ML-DmD21  (54) wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11  27 antennal disc ML-DmD20-c5  (67) mixed discs ML-DmD4-c1  (35) CNS ML-DmBG1-c1  (37) CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    12 ovary OSC  28 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    48 embryonic 1182-4H    30 embryonic GM2    19 embryonic Kc167    26 embryonic S1    38 embryonic S3    45 leg disc CME L1    33 wing disc CME-W2    45 wing disc ML-DmD8    35 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    54 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    67 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    12 ovary OSC    28 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    70 Schneider line 2 Sg4    48 embryonic 1182-4H    30 embryonic GM2    19 embryonic Kc167    26 embryonic S1    38 embryonic S3    45 leg disc CME L1    33 wing disc CME-W2    45 wing disc ML-DmD8    35 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    54 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    67 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    12 ovary OSC    28 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    13 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    13 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    13 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    13 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    13 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    13 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    13 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    13 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • IP21274 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035120 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG13879-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable wacΔ12 Sterility Allele female sterile wacΔ12 Other Phenotypes Allele mitotic cell cycle defective wacΔ12 Phenotype manifest in Allele adult cuticle wacΔ12 chromosome wacΔ12 eye wacΔ12 macrochaeta wacΔ12 spindle wacΔ12
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of wac Class Mutagen Stocks Known lesion wace01983 1 -- wacEY05778 0 Yes wacunspecified 0 -- wacΔ12 P-element activity 0 Yes wacΔ44 P-element activity 0 Yes wacΔ53 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of wac Class Mutagen Stocks Known lesion wacKK112545 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes wacGD12835 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes wacNIG.13879R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)BSC125 (Meireles et al., 2009) Df(3L)Exel6084 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK112545} NA P{NIG.13879R} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}EP3185 NA P{EPgy2}EY05778 NA P{EPgy2}wacEY05778 NA miscellaneous insertions PBac{RB}wace01983 NA
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 11 terms )
Molecular Function
CV term Evidence References
microtubule binding inferred from direct assay (Meireles et al., 2009) protein binding inferred from physical interaction with dgt2 (Meireles et al., 2009)
Biological Process
CV term Evidence References
cell division inferred from mutant phenotype (Meireles et al., 2009) cytoplasmic microtubule organization inferred from mutant phenotype (Hughes et al., 2008) female meiosis inferred from mutant phenotype (Meireles et al., 2009) mitotic spindle organization inferred from mutant phenotype (Hughes et al., 2008) spindle assembly inferred from mutant phenotype (Meireles et al., 2009)
Cellular Component
CV term Evidence References
colocalizes_with HAUS complex inferred from direct assay (Guruharsha et al., 2011) microtubule associated complex inferred from direct assay (Hughes et al., 2008) protein complex inferred from direct assay (Meireles et al., 2009) inferred from genetic interaction with dgt2 (Meireles et al., 2009) spindle inferred from direct assay (Meireles et al., 2009)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
wac - dgt2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based, pull down, autoradiography, anti bait coimmunoprecipitation, western blot (Mintseris et al., 2009.11.23, Guruharsha et al., 2011, Meireles et al., 2009) wac - dgt3 experimental knowledge based (Guruharsha et al., 2011) wac - dgt4 experimental knowledge based (Guruharsha et al., 2011) wac - dgt5 experimental knowledge based (Guruharsha et al., 2011) wac - dgt6 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) wac - msd1 experimental knowledge based (Guruharsha et al., 2011) wac - msd5 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions wac allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63602 DroID - A comprehensive database of gene and protein interactions. • FBgn0035120 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38044
Orthologs
OrthoDB Orthologs (11) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG67WP87 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG67WP87)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\wac     Drosophila simulans   Dsim\GD11004 Y   Drosophila sechellia   Dsec\GM14323 Y   Drosophila erecta   Dere\GG14708 Y   Drosophila yakuba   Dyak\GE21071 Y   Drosophila ananassae   Dana\GF25041 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA12594 Y   Drosophila persimilis   Dper\GL16121 Y   Drosophila virilis   Dvir\GJ16091 Y   Drosophila mojavensis   Dmoj\GI12792 Y   Drosophila grimshawi   Dgri\GH15735
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (9) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11004 Drosophila sechellia Dsec\GM14323 Drosophila erecta Dere\GG14708 Drosophila yakuba Dyak\GE21071 Drosophila ananassae Dana\GF25041 Drosophila pseudoobscura pseudoobscura Dpse\GA12594 Drosophila persimilis Dper\GL16121 Drosophila virilis Dvir\GJ16091 Drosophila mojavensis Dmoj\GI12792
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Harvard	- PBac{RB}wace01983
VDRC	v104962 P{KK112545}VIE-260B
Genomic Clones ( 2 )
	BACR11J11 BACR13F06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 5 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones	IP21074 IP21174 IP21274
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	62839 EK188625
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0035120 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38044
Antibody Information
	polyclonal (Meireles et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: wac CG13879 (Meireles et al., 2009)
Source for database merge of
Additional comments
Other Comments
	wac is not required for acentrosomal spindle microtubule assembly but for the establishment of the forces allowing proper chromosome alignment and segregation in female meiosis. (Meireles et al., 2009) Loss of wac has a significant but limited impact on somatic mitosis and male meiosis based on the apparent lack of cell division failure in imaginal tissues and the largely correct segregation of the chromosomes in larval CNSs and testes. (Meireles et al., 2009)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_138176 NM_001169835 NM_001274265 RefSeq (Proteins) • NP_612020 NP_001163306 NP_001261194 DDBJ / EMBL / GenBank • AAF47365 ACZ94578 AGB93889 Entrez Gene - A searchable database of RefSeq genes. • 38044 UniProtKB/TrEMBL • E1JHV2 Q9W0S8
Other Crossreferences
	BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • IP21274
Linkouts
	BioGRID - A database of protein and genetic interactions • 63602 DroID - A comprehensive database of gene and protein interactions. • FBgn0035120 DRSC - Results from RNAi screens. • FBgn0035120 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035120 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG13879-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0035120 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38044 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38044 modMine - Data generated by the modENCODE project. • FBgn0035120
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG13879 (Meireles et al., 2009, Englund et al., 2006, Hughes et al., 2008, Keleman et al., 2009.8.5, Japanese National Institute of Genetics, 2012.5.21) Wac (Meireles et al., 2009, Wainman et al., 2009) wac (Meireles et al., 2009, Guruharsha et al., 2011, Reschen et al., 2012) was (Meireles et al., 2009)
Name Synonym	CG13879   wee Augmin (Meireles et al., 2009)
Secondary FlyBase IDs
References ( 21 )
Generate a list of	All references relating to this gene ( 21 )
List References by type	Research paper  ( 7 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 5 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) Website  ( 1 )
Recent research papers ( 2 )
	Reschen et al., 2012, Biol. Open 1(5): 422--429 Dgp71WD is required for the assembly of the acentrosomal Meiosis I spindle, and is not a general targeting factor for the γ-TuRC. [FBrf0220171] Guruharsha et al., 2011, Cell 147(3): 690--703 A Protein Complex Network of Drosophila melanogaster. [FBrf0216491] Show all research papers ...