Gene Dmel\Gale

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General Information

Symbol

Dmel\Gale

Species

D. melanogaster

Name

UDP-galactose 4'-epimerase

Annotation symbol

CG12030

Feature type

protein_coding_gene

FlyBase ID

FBgn0035147

Gene Model Status

Current

Stock availability

9 publicly available

Also Known As

dGALE

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

61C8-61C8

Sequence location

3L:648,555..651,311 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene UDP-galactose 4'-epimerase is referred to in FlyBase by the symbol Dmel\Gale (CG12030, FBgn0035147). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: UDP-glucose 4-epimerase activity. There is experimental evidence that it is involved in the biological process: galactose metabolic process. 9 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: NAD(P)-binding domain; NAD-dependent epimerase/dehydratase; Nucleotide sugar epimerase; UDP-glucose 4-epimerase; UDP-glucose 4-epimerase C-terminal domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 12-24 hour embryonic stages, during late pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval/adult midgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, adult spermathecae, adult male accessory gland, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult hindgut, larval fat body, adult ovary, adult testis, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1638409_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG12030. Gene sequence location is 3L:648555..651311.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

61C8-61C8

Limits computationally determined from genome sequence between P{PZ}l(3)05967⁰⁵⁹⁶⁷ and P{PZ}l(3)02640⁰²⁶⁴⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Gale for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

DGCclone may be problematic (AT27946): chimeric?

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Gale-RA

FBtr0072556

NM_138200

1757

350

Gale-RB

FBtr0306917

NM_001259608

1538

350

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Gale-PA

FBpp0072455

38.7

350

7.52

NP_612044

AAF47398

Gale-PB

FBpp0297771

38.7

350

7.52

NP_001246537

AFH04208

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

NAD-dependent epimerase/dehydratase (IPR001509)

UDP-glucose 4-epimerase GalE (IPR005886)

Nucleotide sugar epimerase (IPR008089)

UDP-glucose 4-epimerase C-terminal domain (IPR025308)

NAD(P)-binding domain (IPR016040)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9W0P5

UniProtKB/TrEMBL

Q8MZ78

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 10

amnioserosa

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 11 -- 12

amnioserosa

(Fisher et al., 2012)

plasmatocyte primordium

(Fisher et al., 2012)

Comment:reported as plasmatocytes anlage

posterior midgut primordium

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 13 -- 16

apoptotic amnioserosa

(Fisher et al., 2012)

embryonic proventriculus

(Fisher et al., 2012)

embryonic/larval lymph gland

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval pericardial cell

(Fisher et al., 2012)

embryonic/larval salivary gland

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval/adult midgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, adult spermathecae, adult male accessory gland, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult hindgut, larval fat body, adult ovary, adult testis, larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		100.65
Larval Midgut		722.7
Larval Hindgut		257.4
Larval Malpighian Tubules		833.7
Larval Fat Body		320.3
Larval Salivary Gland		6325.6
Larval Trachea		58.65
Larval Carcass		131.775
Adult Head		423.9
Adult Eye		181.925
Adult Brain		168.9
Adult Thoracic-Abdominal Ganglion		185.1
Adult Crop		285
Adult Midgut		908.8
Adult Hindgut		318.2
Adult Malpighian Tubules		2402.3
Adult Fat Body		1635.9
Adult Salivary Gland		3995.1
Adult Heart		797.575
Adult VirginFemale Spermatheca		1544.9
Adult InseminatedFemale Spermatheca		1000.8
Adult Ovary		170
Adult Testis		107.3
Adult Male Accessory Gland		828.7
Adult Carcass		1183.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		100.65
Larval Midgut		(722.7)
Larval Hindgut		257.4
Larval Malpighian Tubules		(833.7)
Larval Fat Body		320.3
Larval Salivary Gland		(6325.6)
Larval Trachea		58.65
Larval Carcass		131.775
Adult Head		423.9
Adult Eye		181.925
Adult Brain		168.9
Adult Thoracic-Abdominal Ganglion		185.1
Adult Crop		285
Adult Midgut		(908.8)
Adult Hindgut		318.2
Adult Malpighian Tubules		(2402.3)
Adult Fat Body		(1635.9)
Adult Salivary Gland		(3995.1)
Adult Heart		(797.575)
Adult VirginFemale Spermatheca		(1544.9)
Adult InseminatedFemale Spermatheca		(1000.8)
Adult Ovary		170
Adult Testis		107.3
Adult Male Accessory Gland		(828.7)
Adult Carcass		(1183.2)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		100.65
Larval Midgut		722.7
Larval Hindgut		257.4
Larval Malpighian Tubules		833.7
Larval Fat Body		320.3
Larval Salivary Gland		(6325.6)
Larval Trachea		58.65
Larval Carcass		131.775
Adult Head		423.9
Adult Eye		181.925
Adult Brain		168.9
Adult Thoracic-Abdominal Ganglion		185.1
Adult Crop		285
Adult Midgut		908.8
Adult Hindgut		318.2
Adult Malpighian Tubules		(2402.3)
Adult Fat Body		(1635.9)
Adult Salivary Gland		(3995.1)
Adult Heart		797.575
Adult VirginFemale Spermatheca		(1544.9)
Adult InseminatedFemale Spermatheca		1000.8
Adult Ovary		170
Adult Testis		107.3
Adult Male Accessory Gland		828.7
Adult Carcass		(1183.2)
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		100.65
Larval Midgut		722.7
Larval Hindgut		257.4
Larval Malpighian Tubules		833.7
Larval Fat Body		320.3
Larval Salivary Gland		6325.6
Larval Trachea		58.65
Larval Carcass		131.775
Adult Head		423.9
Adult Eye		181.925
Adult Brain		168.9
Adult Thoracic-Abdominal Ganglion		185.1
Adult Crop		285
Adult Midgut		908.8
Adult Hindgut		318.2
Adult Malpighian Tubules		2402.3
Adult Fat Body		1635.9
Adult Salivary Gland		3995.1
Adult Heart		797.575
Adult VirginFemale Spermatheca		1544.9
Adult InseminatedFemale Spermatheca		1000.8
Adult Ovary		170
Adult Testis		107.3
Adult Male Accessory Gland		828.7
Adult Carcass		1183.2
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		100.65
Larval Midgut		722.7
Larval Hindgut		257.4
Larval Malpighian Tubules		833.7
Larval Fat Body		320.3
Larval Salivary Gland		6325.6
Larval Trachea		58.65
Larval Carcass		131.775
Adult Head		423.9
Adult Eye		181.925
Adult Brain		168.9
Adult Thoracic-Abdominal Ganglion		185.1
Adult Crop		285
Adult Midgut		908.8
Adult Hindgut		318.2
Adult Malpighian Tubules		2402.3
Adult Fat Body		1635.9
Adult Salivary Gland		3995.1
Adult Heart		797.575
Adult VirginFemale Spermatheca		1544.9
Adult InseminatedFemale Spermatheca		1000.8
Adult Ovary		170
Adult Testis		107.3
Adult Male Accessory Gland		828.7
Adult Carcass		1183.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		100.65
Larval Midgut		722.7
Larval Hindgut		257.4
Larval Malpighian Tubules		(833.7)
Larval Fat Body		320.3
Larval Salivary Gland		(6325.6)
Larval Trachea		58.65
Larval Carcass		131.775
Adult Head		423.9
Adult Eye		181.925
Adult Brain		168.9
Adult Thoracic-Abdominal Ganglion		185.1
Adult Crop		285
Adult Midgut		(908.8)
Adult Hindgut		318.2
Adult Malpighian Tubules		(2402.3)
Adult Fat Body		(1635.9)
Adult Salivary Gland		(3995.1)
Adult Heart		797.575
Adult VirginFemale Spermatheca		(1544.9)
Adult InseminatedFemale Spermatheca		(1000.8)
Adult Ovary		170
Adult Testis		107.3
Adult Male Accessory Gland		(828.7)
Adult Carcass		(1183.2)
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		100.65
Larval Midgut		722.7
Larval Hindgut		257.4
Larval Malpighian Tubules		833.7
Larval Fat Body		320.3
Larval Salivary Gland		(6325.6)
Larval Trachea		58.65
Larval Carcass		131.775
Adult Head		423.9
Adult Eye		181.925
Adult Brain		168.9
Adult Thoracic-Abdominal Ganglion		185.1
Adult Crop		285
Adult Midgut		908.8
Adult Hindgut		318.2
Adult Malpighian Tubules		(2402.3)
Adult Fat Body		1635.9
Adult Salivary Gland		(3995.1)
Adult Heart		797.575
Adult VirginFemale Spermatheca		1544.9
Adult InseminatedFemale Spermatheca		1000.8
Adult Ovary		170
Adult Testis		107.3
Adult Male Accessory Gland		828.7
Adult Carcass		1183.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		100.65
Larval Midgut		722.7
Larval Hindgut		257.4
Larval Malpighian Tubules		833.7
Larval Fat Body		320.3
Larval Salivary Gland		6325.6
Larval Trachea		58.65
Larval Carcass		131.775
Adult Head		423.9
Adult Eye		181.925
Adult Brain		168.9
Adult Thoracic-Abdominal Ganglion		185.1
Adult Crop		285
Adult Midgut		908.8
Adult Hindgut		318.2
Adult Malpighian Tubules		2402.3
Adult Fat Body		1635.9
Adult Salivary Gland		3995.1
Adult Heart		797.575
Adult VirginFemale Spermatheca		1544.9
Adult InseminatedFemale Spermatheca		1000.8
Adult Ovary		170
Adult Testis		107.3
Adult Male Accessory Gland		828.7
Adult Carcass		1183.2
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	423.9
NA	Eye	181.925
NA	Brain	168.9
100.65	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	185.1
NA	Crop	285
722.7	Midgut	908.8
257.4	Hindgut	318.2
833.7	Malpighian Tubules	2402.3
320.3	Fat Body	1635.9
6325.6	Salivary Gland	3995.1
NA	Heart	797.575
58.65	Trachea	NA
NA	VirginFemale Spermatheca	1544.9
NA	InseminatedFemale Spermatheca	1000.8
NA	Ovary	170
NA	Testis	107.3
NA	Male Accessory Gland	828.7
131.775	Carcass	1183.2

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		25
central nervous system, larvae L3		10
central nervous system, pupae P8		6
head, virgin 1-day female		28
head, virgin 4-day female		19
head, virgin 20-day female		42
head, mated 1-day female		14
head, mated 4-day female		19
head, mated 20-day female		29
head, mated 1-day male		27
head, mated 4-day male		28
head, mated 20-day male		38
salivary gland, larvae L3 wandering		79
salivary gland, white prepupae		37
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		31
digestive system, 4-day adult		22
digestive system, 20-day adult		32
fat body, larvae L3 wandering		13
fat body, white prepupae		5
fat body, pupae P8		16
carcass, larvae L3 wandering		9
carcass, 1-day adult		24
carcass, 4-day adult		24
carcass, 20-day adult		37
ovary, virgin 4-day female		10
ovary, mated 4-day female		9
testis, mated 4-day male		16
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		25
central nervous system, larvae L3		10
central nervous system, pupae P8		6
head, virgin 1-day female		(28)
head, virgin 4-day female		19
head, virgin 20-day female		(42)
head, mated 1-day female		14
head, mated 4-day female		19
head, mated 20-day female		(29)
head, mated 1-day male		(27)
head, mated 4-day male		(28)
head, mated 20-day male		(38)
salivary gland, larvae L3 wandering		(79)
salivary gland, white prepupae		(37)
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		(31)
digestive system, 4-day adult		22
digestive system, 20-day adult		(32)
fat body, larvae L3 wandering		13
fat body, white prepupae		5
fat body, pupae P8		16
carcass, larvae L3 wandering		9
carcass, 1-day adult		24
carcass, 4-day adult		24
carcass, 20-day adult		(37)
ovary, virgin 4-day female		10
ovary, mated 4-day female		9
testis, mated 4-day male		16
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		25
central nervous system, larvae L3		10
central nervous system, pupae P8		6
head, virgin 1-day female		28
head, virgin 4-day female		19
head, virgin 20-day female		42
head, mated 1-day female		14
head, mated 4-day female		19
head, mated 20-day female		29
head, mated 1-day male		27
head, mated 4-day male		28
head, mated 20-day male		38
salivary gland, larvae L3 wandering		79
salivary gland, white prepupae		37
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		31
digestive system, 4-day adult		22
digestive system, 20-day adult		32
fat body, larvae L3 wandering		13
fat body, white prepupae		5
fat body, pupae P8		16
carcass, larvae L3 wandering		9
carcass, 1-day adult		24
carcass, 4-day adult		24
carcass, 20-day adult		37
ovary, virgin 4-day female		10
ovary, mated 4-day female		9
testis, mated 4-day male		16
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		25
central nervous system, larvae L3		10
central nervous system, pupae P8		6
head, virgin 1-day female		28
head, virgin 4-day female		19
head, virgin 20-day female		42
head, mated 1-day female		14
head, mated 4-day female		19
head, mated 20-day female		29
head, mated 1-day male		27
head, mated 4-day male		28
head, mated 20-day male		38
salivary gland, larvae L3 wandering		79
salivary gland, white prepupae		37
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		31
digestive system, 4-day adult		22
digestive system, 20-day adult		32
fat body, larvae L3 wandering		13
fat body, white prepupae		5
fat body, pupae P8		16
carcass, larvae L3 wandering		9
carcass, 1-day adult		24
carcass, 4-day adult		24
carcass, 20-day adult		37
ovary, virgin 4-day female		10
ovary, mated 4-day female		9
testis, mated 4-day male		16
accessory gland, mated 4-day male		26
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		25
central nervous system, larvae L3		10
central nervous system, pupae P8		6
head, virgin 1-day female		28
head, virgin 4-day female		19
head, virgin 20-day female		42
head, mated 1-day female		14
head, mated 4-day female		19
head, mated 20-day female		29
head, mated 1-day male		27
head, mated 4-day male		28
head, mated 20-day male		38
salivary gland, larvae L3 wandering		79
salivary gland, white prepupae		37
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		31
digestive system, 4-day adult		22
digestive system, 20-day adult		32
fat body, larvae L3 wandering		13
fat body, white prepupae		5
fat body, pupae P8		16
carcass, larvae L3 wandering		9
carcass, 1-day adult		24
carcass, 4-day adult		24
carcass, 20-day adult		37
ovary, virgin 4-day female		10
ovary, mated 4-day female		9
testis, mated 4-day male		16
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		25
central nervous system, larvae L3		10
central nervous system, pupae P8		6
head, virgin 1-day female		28
head, virgin 4-day female		19
head, virgin 20-day female		(42)
head, mated 1-day female		14
head, mated 4-day female		19
head, mated 20-day female		29
head, mated 1-day male		27
head, mated 4-day male		28
head, mated 20-day male		(38)
salivary gland, larvae L3 wandering		(79)
salivary gland, white prepupae		(37)
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		31
digestive system, 4-day adult		22
digestive system, 20-day adult		32
fat body, larvae L3 wandering		13
fat body, white prepupae		5
fat body, pupae P8		16
carcass, larvae L3 wandering		9
carcass, 1-day adult		24
carcass, 4-day adult		24
carcass, 20-day adult		(37)
ovary, virgin 4-day female		10
ovary, mated 4-day female		9
testis, mated 4-day male		16
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		25
central nervous system, larvae L3		10
central nervous system, pupae P8		6
head, virgin 1-day female		28
head, virgin 4-day female		19
head, virgin 20-day female		42
head, mated 1-day female		14
head, mated 4-day female		19
head, mated 20-day female		29
head, mated 1-day male		27
head, mated 4-day male		28
head, mated 20-day male		38
salivary gland, larvae L3 wandering		79
salivary gland, white prepupae		37
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		31
digestive system, 4-day adult		22
digestive system, 20-day adult		32
fat body, larvae L3 wandering		13
fat body, white prepupae		5
fat body, pupae P8		16
carcass, larvae L3 wandering		9
carcass, 1-day adult		24
carcass, 4-day adult		24
carcass, 20-day adult		37
ovary, virgin 4-day female		10
ovary, mated 4-day female		9
testis, mated 4-day male		16
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		25
central nervous system, larvae L3		10
central nervous system, pupae P8		6
head, virgin 1-day female		28
head, virgin 4-day female		19
head, virgin 20-day female		42
head, mated 1-day female		14
head, mated 4-day female		19
head, mated 20-day female		29
head, mated 1-day male		27
head, mated 4-day male		28
head, mated 20-day male		38
salivary gland, larvae L3 wandering		79
salivary gland, white prepupae		37
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		31
digestive system, 4-day adult		22
digestive system, 20-day adult		32
fat body, larvae L3 wandering		13
fat body, white prepupae		5
fat body, pupae P8		16
carcass, larvae L3 wandering		9
carcass, 1-day adult		24
carcass, 4-day adult		24
carcass, 20-day adult		37
ovary, virgin 4-day female		10
ovary, mated 4-day female		9
testis, mated 4-day male		16
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 12-24 hour embryonic stages, during late pupal stages, in adult male stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		17
embryo 04-06hr		34
embryo 06-08hr		95
embryo 08-10hr		94
embryo 10-12hr		120
embryo 12-14hr		92
embryo 14-16hr		123
embryo 16-18hr		151
embryo 18-20hr		244
embryo 20-22hr		188
embryo 22-24hr		189
larva L1		92
larva L2		106
larva L3 12hr old		108
larva L3 puffstage 1-2		114
larva L3 puffstage 3-6		77
larva L3 puffstage 7-9		45
white prepupae new		33
white prepupae 12hr		72
white prepupae 24hr		36
pupae 2d postWPP		27
pupae 3d postWPP		27
pupae 4d postWPP		190
adult male 01day		177
adult male 05day		99
adult male 30day		99
adult female 01day		127
adult female 05day		82
adult female 30day		85
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		17
embryo 04-06hr		(34)
embryo 06-08hr		(95)
embryo 08-10hr		(94)
embryo 10-12hr		(120)
embryo 12-14hr		(92)
embryo 14-16hr		(123)
embryo 16-18hr		(151)
embryo 18-20hr		(244)
embryo 20-22hr		(188)
embryo 22-24hr		(189)
larva L1		(92)
larva L2		(106)
larva L3 12hr old		(108)
larva L3 puffstage 1-2		(114)
larva L3 puffstage 3-6		(77)
larva L3 puffstage 7-9		(45)
white prepupae new		(33)
white prepupae 12hr		(72)
white prepupae 24hr		(36)
pupae 2d postWPP		(27)
pupae 3d postWPP		(27)
pupae 4d postWPP		(190)
adult male 01day		(177)
adult male 05day		(99)
adult male 30day		(99)
adult female 01day		(127)
adult female 05day		(82)
adult female 30day		(85)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		17
embryo 04-06hr		34
embryo 06-08hr		95
embryo 08-10hr		94
embryo 10-12hr		(120)
embryo 12-14hr		92
embryo 14-16hr		(123)
embryo 16-18hr		(151)
embryo 18-20hr		(244)
embryo 20-22hr		(188)
embryo 22-24hr		(189)
larva L1		92
larva L2		106
larva L3 12hr old		(108)
larva L3 puffstage 1-2		(114)
larva L3 puffstage 3-6		77
larva L3 puffstage 7-9		45
white prepupae new		33
white prepupae 12hr		72
white prepupae 24hr		36
pupae 2d postWPP		27
pupae 3d postWPP		27
pupae 4d postWPP		(190)
adult male 01day		(177)
adult male 05day		99
adult male 30day		99
adult female 01day		(127)
adult female 05day		82
adult female 30day		85
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		17
embryo 04-06hr		34
embryo 06-08hr		95
embryo 08-10hr		94
embryo 10-12hr		120
embryo 12-14hr		92
embryo 14-16hr		123
embryo 16-18hr		151
embryo 18-20hr		244
embryo 20-22hr		188
embryo 22-24hr		189
larva L1		92
larva L2		106
larva L3 12hr old		108
larva L3 puffstage 1-2		114
larva L3 puffstage 3-6		77
larva L3 puffstage 7-9		45
white prepupae new		33
white prepupae 12hr		72
white prepupae 24hr		36
pupae 2d postWPP		27
pupae 3d postWPP		27
pupae 4d postWPP		190
adult male 01day		177
adult male 05day		99
adult male 30day		99
adult female 01day		127
adult female 05day		82
adult female 30day		85
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		17
embryo 04-06hr		34
embryo 06-08hr		95
embryo 08-10hr		94
embryo 10-12hr		120
embryo 12-14hr		92
embryo 14-16hr		123
embryo 16-18hr		151
embryo 18-20hr		244
embryo 20-22hr		188
embryo 22-24hr		189
larva L1		92
larva L2		106
larva L3 12hr old		108
larva L3 puffstage 1-2		114
larva L3 puffstage 3-6		77
larva L3 puffstage 7-9		45
white prepupae new		33
white prepupae 12hr		72
white prepupae 24hr		36
pupae 2d postWPP		27
pupae 3d postWPP		27
pupae 4d postWPP		190
adult male 01day		177
adult male 05day		99
adult male 30day		99
adult female 01day		127
adult female 05day		82
adult female 30day		85
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		17
embryo 04-06hr		(34)
embryo 06-08hr		(95)
embryo 08-10hr		(94)
embryo 10-12hr		(120)
embryo 12-14hr		(92)
embryo 14-16hr		(123)
embryo 16-18hr		(151)
embryo 18-20hr		(244)
embryo 20-22hr		(188)
embryo 22-24hr		(189)
larva L1		(92)
larva L2		(106)
larva L3 12hr old		(108)
larva L3 puffstage 1-2		(114)
larva L3 puffstage 3-6		(77)
larva L3 puffstage 7-9		(45)
white prepupae new		(33)
white prepupae 12hr		(72)
white prepupae 24hr		(36)
pupae 2d postWPP		27
pupae 3d postWPP		27
pupae 4d postWPP		(190)
adult male 01day		(177)
adult male 05day		(99)
adult male 30day		(99)
adult female 01day		(127)
adult female 05day		(82)
adult female 30day		(85)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		17
embryo 04-06hr		34
embryo 06-08hr		95
embryo 08-10hr		94
embryo 10-12hr		120
embryo 12-14hr		92
embryo 14-16hr		123
embryo 16-18hr		(151)
embryo 18-20hr		(244)
embryo 20-22hr		(188)
embryo 22-24hr		(189)
larva L1		92
larva L2		106
larva L3 12hr old		108
larva L3 puffstage 1-2		114
larva L3 puffstage 3-6		77
larva L3 puffstage 7-9		45
white prepupae new		33
white prepupae 12hr		72
white prepupae 24hr		36
pupae 2d postWPP		27
pupae 3d postWPP		27
pupae 4d postWPP		(190)
adult male 01day		(177)
adult male 05day		99
adult male 30day		99
adult female 01day		127
adult female 05day		82
adult female 30day		85
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		17
embryo 04-06hr		34
embryo 06-08hr		95
embryo 08-10hr		94
embryo 10-12hr		120
embryo 12-14hr		92
embryo 14-16hr		123
embryo 16-18hr		151
embryo 18-20hr		244
embryo 20-22hr		188
embryo 22-24hr		189
larva L1		92
larva L2		106
larva L3 12hr old		108
larva L3 puffstage 1-2		114
larva L3 puffstage 3-6		77
larva L3 puffstage 7-9		45
white prepupae new		33
white prepupae 12hr		72
white prepupae 24hr		36
pupae 2d postWPP		27
pupae 3d postWPP		27
pupae 4d postWPP		190
adult male 01day		177
adult male 05day		99
adult male 30day		99
adult female 01day		127
adult female 05day		82
adult female 30day		85
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		371
Schneider line 2 Sg4		117
embryonic 1182-4H		231
embryonic GM2		218
embryonic Kc167		84
embryonic S1		59
embryonic S3		102
leg disc CME L1		89
wing disc CME-W2		41
wing disc ML-DmD8		137
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		33
wing disc ML-DmD21		44
wing disc ML-DmD32		64
haltere disc ML-DmD17-c3		2
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		31
mixed discs ML-DmD4-c1		80
CNS ML-DmBG1-c1		112
CNS ML-DmBG2-c2		8
tumorous blood cells mbn2		139
ovary fGS/OSS		4
ovary OSC		5
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(371)
Schneider line 2 Sg4		(117)
embryonic 1182-4H		(231)
embryonic GM2		(218)
embryonic Kc167		(84)
embryonic S1		(59)
embryonic S3		(102)
leg disc CME L1		(89)
wing disc CME-W2		(41)
wing disc ML-DmD8		(137)
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		(33)
wing disc ML-DmD21		(44)
wing disc ML-DmD32		(64)
haltere disc ML-DmD17-c3		2
eye-antennal disc ML-DmD11		(31)
antennal disc ML-DmD20-c5		(31)
mixed discs ML-DmD4-c1		(80)
CNS ML-DmBG1-c1		(112)
CNS ML-DmBG2-c2		8
tumorous blood cells mbn2		(139)
ovary fGS/OSS		4
ovary OSC		5
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(371)
Schneider line 2 Sg4		(117)
embryonic 1182-4H		(231)
embryonic GM2		(218)
embryonic Kc167		84
embryonic S1		59
embryonic S3		102
leg disc CME L1		89
wing disc CME-W2		41
wing disc ML-DmD8		(137)
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		33
wing disc ML-DmD21		44
wing disc ML-DmD32		64
haltere disc ML-DmD17-c3		2
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		31
mixed discs ML-DmD4-c1		80
CNS ML-DmBG1-c1		(112)
CNS ML-DmBG2-c2		8
tumorous blood cells mbn2		(139)
ovary fGS/OSS		4
ovary OSC		5
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		371
Schneider line 2 Sg4		117
embryonic 1182-4H		231
embryonic GM2		218
embryonic Kc167		84
embryonic S1		59
embryonic S3		102
leg disc CME L1		89
wing disc CME-W2		41
wing disc ML-DmD8		137
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		33
wing disc ML-DmD21		44
wing disc ML-DmD32		64
haltere disc ML-DmD17-c3		2
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		31
mixed discs ML-DmD4-c1		80
CNS ML-DmBG1-c1		112
CNS ML-DmBG2-c2		8
tumorous blood cells mbn2		139
ovary fGS/OSS		4
ovary OSC		5
ovary OSS		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		371
Schneider line 2 Sg4		117
embryonic 1182-4H		231
embryonic GM2		218
embryonic Kc167		84
embryonic S1		59
embryonic S3		102
leg disc CME L1		89
wing disc CME-W2		41
wing disc ML-DmD8		137
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		33
wing disc ML-DmD21		44
wing disc ML-DmD32		64
haltere disc ML-DmD17-c3		2
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		31
mixed discs ML-DmD4-c1		80
CNS ML-DmBG1-c1		112
CNS ML-DmBG2-c2		8
tumorous blood cells mbn2		139
ovary fGS/OSS		4
ovary OSC		5
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(371)
Schneider line 2 Sg4		(117)
embryonic 1182-4H		(231)
embryonic GM2		(218)
embryonic Kc167		(84)
embryonic S1		(59)
embryonic S3		(102)
leg disc CME L1		(89)
wing disc CME-W2		(41)
wing disc ML-DmD8		(137)
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		(33)
wing disc ML-DmD21		(44)
wing disc ML-DmD32		(64)
haltere disc ML-DmD17-c3		2
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		31
mixed discs ML-DmD4-c1		(80)
CNS ML-DmBG1-c1		(112)
CNS ML-DmBG2-c2		8
tumorous blood cells mbn2		(139)
ovary fGS/OSS		4
ovary OSC		5
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(371)
Schneider line 2 Sg4		117
embryonic 1182-4H		(231)
embryonic GM2		(218)
embryonic Kc167		84
embryonic S1		59
embryonic S3		102
leg disc CME L1		89
wing disc CME-W2		41
wing disc ML-DmD8		137
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		33
wing disc ML-DmD21		44
wing disc ML-DmD32		64
haltere disc ML-DmD17-c3		2
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		31
mixed discs ML-DmD4-c1		80
CNS ML-DmBG1-c1		112
CNS ML-DmBG2-c2		8
tumorous blood cells mbn2		139
ovary fGS/OSS		4
ovary OSC		5
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		371
Schneider line 2 Sg4		117
embryonic 1182-4H		231
embryonic GM2		218
embryonic Kc167		84
embryonic S1		59
embryonic S3		102
leg disc CME L1		89
wing disc CME-W2		41
wing disc ML-DmD8		137
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		33
wing disc ML-DmD21		44
wing disc ML-DmD32		64
haltere disc ML-DmD17-c3		2
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		31
mixed discs ML-DmD4-c1		80
CNS ML-DmBG1-c1		112
CNS ML-DmBG2-c2		8
tumorous blood cells mbn2		139
ovary fGS/OSS		4
ovary OSC		5
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		12
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		77
Cadmium 50 mM 12 hrs, larvae L3		37
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		37
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		36
Ethanol 5% 3 hrs, larvae L3		53
Ethanol 10% 3 hrs, larvae L3		61
Caffeine 1.5 mg/ml 4 hrs, larvae L3		49
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		11
Paraquat 5 mM 48 hrs, 4-day adult		23
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		60
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		12
heat shock, 4-day adult		(44)
Cadmium 50 mM 6 hrs, larvae L3		(77)
Cadmium 50 mM 12 hrs, larvae L3		(37)
Cadmium 50 mM 48 hrs, 4-day adult		(41)
Cadmium 100 mM 48 hrs, 4-day adult		(27)
Copper 0.5 mM 12 hrs, larvae L3		(37)
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		(36)
Ethanol 5% 3 hrs, larvae L3		(53)
Ethanol 10% 3 hrs, larvae L3		(61)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(49)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		11
Paraquat 5 mM 48 hrs, 4-day adult		23
Paraquat 10 mM 48 hrs, 4-day adult		(28)
Rotenone 2 μg 12 hrs, larvae L3		(60)
Rotenone 8 μg 12 hrs, larvae L3		(53)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		12
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		77
Cadmium 50 mM 12 hrs, larvae L3		37
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		37
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		36
Ethanol 5% 3 hrs, larvae L3		53
Ethanol 10% 3 hrs, larvae L3		61
Caffeine 1.5 mg/ml 4 hrs, larvae L3		49
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		11
Paraquat 5 mM 48 hrs, 4-day adult		23
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		60
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		12
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		77
Cadmium 50 mM 12 hrs, larvae L3		37
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		37
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		36
Ethanol 5% 3 hrs, larvae L3		53
Ethanol 10% 3 hrs, larvae L3		61
Caffeine 1.5 mg/ml 4 hrs, larvae L3		49
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		11
Paraquat 5 mM 48 hrs, 4-day adult		23
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		60
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		12
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		77
Cadmium 50 mM 12 hrs, larvae L3		37
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		37
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		36
Ethanol 5% 3 hrs, larvae L3		53
Ethanol 10% 3 hrs, larvae L3		61
Caffeine 1.5 mg/ml 4 hrs, larvae L3		49
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		11
Paraquat 5 mM 48 hrs, 4-day adult		23
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		60
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		12
heat shock, 4-day adult		(44)
Cadmium 50 mM 6 hrs, larvae L3		(77)
Cadmium 50 mM 12 hrs, larvae L3		(37)
Cadmium 50 mM 48 hrs, 4-day adult		(41)
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		(37)
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		(36)
Ethanol 5% 3 hrs, larvae L3		(53)
Ethanol 10% 3 hrs, larvae L3		(61)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(49)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		11
Paraquat 5 mM 48 hrs, 4-day adult		23
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		(60)
Rotenone 8 μg 12 hrs, larvae L3		(53)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		12
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		77
Cadmium 50 mM 12 hrs, larvae L3		37
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		37
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		36
Ethanol 5% 3 hrs, larvae L3		53
Ethanol 10% 3 hrs, larvae L3		61
Caffeine 1.5 mg/ml 4 hrs, larvae L3		49
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		11
Paraquat 5 mM 48 hrs, 4-day adult		23
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		60
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		12
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		77
Cadmium 50 mM 12 hrs, larvae L3		37
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		37
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		36
Ethanol 5% 3 hrs, larvae L3		53
Ethanol 10% 3 hrs, larvae L3		61
Caffeine 1.5 mg/ml 4 hrs, larvae L3		49
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		11
Paraquat 5 mM 48 hrs, 4-day adult		23
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		60
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

LD27852

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0035147

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG12030-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Gale

Stages(s) 11-12

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PI

Gale^NIG.12030R

lethal, with Scer\GAL4^drm.7.1

Gale^NIG.12030R

lethal (with Df(3L)ED4196)

lethal (with Df(3L)emc-E12)

lethal (with Gale^f00624)

lethal (with Gale^h)

lethal (with Gale^Δy)

lethal | germline clone | late embryonic stage (with Gale^f00624)

Gale^Δy

lethal | germline clone | late embryonic stage (with Gale^Δy)

Gale^f00624

lethal | heat sensitive, with Scer\GAL4^Act5C.PI, Scer\GAL80^ts.αTub84B

Gale^NIG.12030R

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Gale^GD7464

lethal | recessive

lethal | second instar larval stage (with Gale^f00624)

Gale^Δy

lethal | second instar larval stage (with Gale^Δy)

Gale^f00624

lethal | temperature conditional, with Scer\GAL4^Act5C.PI, Scer\GAL80^ts.αTub84B

Gale^NIG.12030R

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

Gale^GD7464

viable

Sterility

Allele

sterile | temperature conditional, with Scer\GAL4^Act5C.PI, Scer\GAL80^ts.αTub84B

Gale^NIG.12030R

Other Phenotypes

Allele

body color defective, with Scer\GAL4^pnr-MD237

Gale^GD7464

chemical sensitive

Gale^h

short lived | chemical conditional, with Scer\GAL4^Act5C.PI

Gale^NIG.12030R

short lived | temperature conditional, with Scer\GAL4^Act5C.PI, Scer\GAL80^ts.αTub84B

Gale^NIG.12030R

small body | larval stage (with Gale^f00624)

Gale^Δy

small body | larval stage (with Gale^Δy)

Gale^f00624

Phenotype manifest in

Allele

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Gale^GD7464

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of Gale	Class	Mutagen	Stocks	Known lesion
Gale^EY22205		P-element activity	1	--
Gale^f00624	hypomorphic allele - molecular evidence	piggyBac transposase	1	--
Gale^h	hypomorphic allele - genetic evidence	Delta2-3	1	Yes
Gale^Δy	hypomorphic allele - molecular evidence	Delta2-3	1	Yes
Gale^d	wild-type allele	Delta2-3	0	Yes

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Gale	Mutagen	Stocks	Known lesion
Gale^GD7464	in vitro construct - RNAi in vitro construct - regulatory fusion	4	Yes
Gale^KK107540	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Gale^NIG.12030R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Gale^Scer\UAS.cRa	in vitro construct - regulatory fusion	0	Yes