FlyBase Gene Report: Dmel\CG2199

C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Protein Function (UniProtKB)

May be involved in transcriptional regulation.

(UniProt, Q8IRH5)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\CG2199 or the JBrowse view of Dmel\CG2199 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG2199-RA

FBtr0072722

NM_138260

2846

724

CG2199-RB

FBtr0072721

NM_167873

2576

733

CG2199-RC

FBtr0331865

NM_001274323

2640

724

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CG2199-PA

FBpp0072606

80.9

724

8.82

NP_612104

AAF47485

CG2199-PB

FBpp0072605

82.0

733

8.77

NP_728599

AAN11474

CG2199-PC

FBpp0304249

80.9

724

8.82

NP_001261252

AGB93947

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

724 aa isoforms: CG2199-PA, CG2199-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG2199 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG2199_1

Linkouts

Gene Ontology (7 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from sequence or structural similarity with UniProtKB:P32338

(FlyBase Curators, 2008--)

RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from sequence or structural similarity with UniProtKB:P32338

(FlyBase Curators, 2008--)

RNA polymerase II regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN001227969

(assigned by GO_Central )

(Gaudet et al., 2011)

sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN001227969

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

negative regulation of transcription by RNA polymerase II

inferred from sequence or structural similarity with UniProtKB:P32338

(FlyBase Curators, 2008--)

positive regulation of transcription by RNA polymerase II

inferred from sequence or structural similarity with UniProtKB:P32338

(FlyBase Curators, 2008--)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

nucleus

inferred from sequence or structural similarity with UniProtKB:P32338

(FlyBase Curators, 2008--)

inferred from biological aspect of ancestor with PANTHER:PTN001227969

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

gastric caecum

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG2199 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG2199

View exonic expression by tissue for Dmel\CG2199

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035213

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0035213

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2199-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2199

Flygut - An atlas of the Drosophila adult midgut

FBgn0035213

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CG2199

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG2199

Transgenic constructs containing regulatory region of CG2199

Deletions and Duplications ( 1 )

Disrupted in

Df(3L)Ptp61F^4-10

(Tchankouo-Nguetcheu et al., 2014)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG2199^GD9748

viable, with Scer\GAL4^Mef2.PR

CG2199^GD9748

viable, with Scer\GAL4^pnr-MD237

CG2199^GD9748

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

CG2199^GD9748

visible | male limited, with Scer\GAL4^Bx-MS1096

CG2199^{UAS.ORF.GW.Tag:HA}

Phenotype manifest in

Allele

trichogen cell, with Scer\GAL4^pnr-MD237

CG2199^GD9748

wing | male limited, with Scer\GAL4^Bx-MS1096

CG2199^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (21)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\TP63

1 of 15

Yes

Hsap\ZFP92

1 of 15

Yes

Hsap\ZNF48

1 of 15

Yes

Hsap\ZNF84

1 of 15

Yes

Hsap\ZNF91

1 of 15

Yes

Hsap\ZNF99

1 of 15

Yes

Hsap\ZNF107

1 of 15

Yes

Hsap\ZNF160

1 of 15

Yes

Hsap\ZNF181

1 of 15

Yes

Hsap\ZNF184

1 of 15

Yes

Hsap\ZNF208

1 of 15

Yes

Hsap\ZNF302

1 of 15

Yes

Hsap\ZNF347

1 of 15

Yes

Hsap\ZNF497

1 of 15

Yes

Hsap\ZNF665

1 of 15

Yes

Hsap\ZNF688

1 of 15

Yes

Hsap\ZNF689

1 of 15

Yes

Hsap\ZNF729

1 of 15

Yes

Hsap\ZNF747

1 of 15

Yes

Hsap\ZNF764

1 of 15

Yes

Hsap\ZNF785

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\E430018J23Rik

1 of 15

Yes

Mmus\Zfp92

1 of 15

Yes

Mmus\Zfp160

1 of 15

Yes

Mmus\Zfp553

1 of 15

Yes

Mmus\Zfp688

1 of 15

Yes

Mmus\Zfp689

1 of 15

Yes

Mmus\Zfp764

1 of 15

Yes

Rattus norvegicus (Norway rat) (6)

Rnor\LOC100365363

1 of 13

Yes

Rnor\LOC102553866

1 of 13

Yes

Rnor\LOC102553962

1 of 13

Yes

Rnor\LOC102554302

1 of 13

Yes

Rnor\Zfp322a

1 of 13

Yes

Rnor\Znf48

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (0)

No records found.

Danio rerio (Zebrafish) (1)

Drer\im:7147486

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\ces-1

1 of 15

Yes

Cele\ham-2

1 of 15

Yes

Cele\lsy-27

1 of 15

Yes

Cele\ztf-15

1 of 15

Yes

Cele\ztf-25

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091903ZL )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15296

Drosophila persimilis

Dper\GL16070

Drosophila willistoni

Dwil\GK15002

Drosophila willistoni

Dwil\GK12499

Drosophila virilis

Dvir\GJ13203

Drosophila mojavensis

Dmoj\GI13373

Drosophila grimshawi

Dgri\GH16227

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W020L )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI085693

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI24400

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01Y3 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78152.1

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (45)

Gene Symbol

Score

Source

Alignment

az2

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

mld

1 of 10

Neu2

1 of 10

Opbp

1 of 10

Plzf

1 of 10

pzg

1 of 10

Sry-β

1 of 10

wor

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CG2199 - CG3548

experimental knowledge based

(Guruharsha et al., 2011)

CG2199 - Cpsf6

experimental knowledge based

(Guruharsha et al., 2011)

CG2199 - Gnf1

experimental knowledge based

(Guruharsha et al., 2011)

CG2199 - MED11

experimental knowledge based

(Guruharsha et al., 2011)

CG2199 - MED14

experimental knowledge based

(Guruharsha et al., 2011)

CG2199 - MED17

experimental knowledge based

(Guruharsha et al., 2011)

CG2199 - Ote

experimental knowledge based

(Guruharsha et al., 2011)

CG2199 - Pi3K21B

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CG2199 - TFAM

experimental knowledge based

(Guruharsha et al., 2011)

CG2199 - Trl

two hybrid, anti bait coimmunoprecipitation, Identification by mass spectrometry

(Lomaev et al., 2017)

CG2199 - bocks

experimental knowledge based

(Guruharsha et al., 2011)

CG2199 - drm

two hybrid array

(Shokri et al., 2019)

CG2199 - gcm

two hybrid

(Jacques et al., 2009)

CG2199 - kel

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

CG2199 - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63701

DroID - A comprehensive database of gene and protein interactions.

FBgn0035213

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38159

MIST (protein-protein) - An integrated Molecular Interaction Database

CG2199

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-0

Cytogenetic map

61F7-61F7

Sequence location

3L:1,339,460..1,342,403 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

61F7-61F7

Limits computationally determined from genome sequence between P{PZ}l(3)02640⁰²⁶⁴⁰ and P{EP}rho^EP3704&P{PZ}l(3)06226⁰⁶²²⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-0

(FlyBase, 2016)

3-1.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Bloomington

27082

y¹ v¹; P{TRiP.JF02428}attP2

85021

w¹¹¹⁸; PBac{PB}CG2199^c02129

63661

y¹ v¹; P{TRiP.HMJ30228}attP40

67660

y¹ w^*; P{CG2199-GFP.FPTB}attP40

80030

y¹ sc^* v¹ sev²¹; P{TOE.GS02989}attP40

FlyORF

F004543

M{UAS-CG2199.ORF-CC}ZH-21F

F000633

M{UAS-CG2199.ORF.3xHA.GW}ZH-86Fb

VDRC

v20839

w¹¹¹⁸; P{GD9748}v20839

v318138

PBac{fTRG00508.sfGFP-TVPTBF}VK00033

v341424

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00489}attP40/CyO-GFP

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (73)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

FI17108

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0035213

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0035213

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38159

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

FlyBase curator comment: the insertion in the "f06926" Exelixis line (PBac{WH}Ptp61F^f06926) was originally assigned to the CG2199 gene in FBrf0179797, resulting in the "CG2199^f06926" (FBal0161936) allele. However, FBrf0184340 shows that the insertion is actually within Ptp61F.

(Bloomington Drosophila Stock Center, 2004.11.6)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 37 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38159

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8IRH5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PBH8

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035213

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0035213

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG2199_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2199

Flygut - An atlas of the Drosophila adult midgut

FBgn0035213

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38159

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG2199

modMine - A data warehouse for the modENCODE project

FBgn0035213

Linkouts

BioGRID - A database of protein and genetic interactions.

63701

DroID - A comprehensive database of gene and protein interactions.

FBgn0035213

DRSC - Results frm RNAi screens

FBgn0035213

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0035213

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2199-RB

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0035213

FlyMine - An integrated database for Drosophila genomics

FBgn0035213

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38159

MIST (protein-protein) - An integrated Molecular Interaction Database

CG2199

Synonyms and Secondary IDs (2)

Reported As

Name Synonyms

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (53)

Report Sections

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