FB2020_03, released Jun 16, 2020

Gene: Dmel\SCOT

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General Information
Symbol
Dmel\SCOT
Species
D. melanogaster
Name
Succinyl-CoA:3-ketoacid CoA transferase
Annotation Symbol
CG1140
Feature Type
protein_coding_gene
FlyBase ID
FBgn0035298
Gene Model Status
Current
Stock Availability
8 publicly available
Enzyme Name (EC)
3-oxoacid CoA-transferase (2.8.3.5)
Gene Snapshot
Contributions welcome
Other Summaries
Also Known As

3-oxoacid CoA-transferase

Key Links
Genomic Location
Cytogenetic map
62B11-62B12
Sequence location
3L:1,963,143..1,966,805 [+]
Recombination map

3-1.0

RefSeq locus
NT_037436 REGION:1963143..1966805
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (3 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
3-oxoacid CoA-transferase activity
inferred from direct assay
(Zhang et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
3-oxoacid CoA-transferase activity
inferred from sequence or structural similarity with UniProtKB:P55809
(Sardiello et al., 2003.6.11)
inferred from sequence or structural similarity with UniProtKB:Q29551
(Kasravi et al., 1999)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
ketone body catabolic process
inferred from sequence or structural similarity with UniProtKB:P55809
(Sardiello et al., 2003.6.11)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
mitochondrial matrix
inferred from sequence or structural similarity with UniProtKB:P55809
(Sardiello et al., 2003.6.11)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the 3-oxoacid CoA-transferase family. (Q9W058)
Protein Signatures (InterPro)
Catalytic Activity (EC)
Experimental Evidence
Succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA (2.8.3.5)
Predictions / Assertions
Succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA (2.8.3.5)
Summaries
Gene Group (FlyBase)
OTHER SULFUR-CONTAINING GROUP TRANSFERASES -
The Other sulfur-containing group transferases is a collection of transferases that do not fit into any of the other major transferases transferring sulfur-containing groups.
Protein Function (UniProtKB)
Key enzyme for ketone body catabolism (PubMed:24100554). Transfers the CoA moiety from succinate to acetoacetate (PubMed:24100554). Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity).
(UniProt, Q9W058)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\SCOT or the JBrowse view of Dmel\SCOT for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
SCOT-RA
FBtr0072844
NM_139426
1768
516
SCOT-RB
FBtr0072843
NM_167928
1966
502
SCOT-RC
FBtr0333431
NM_001274372
2006
450
Additional Transcript Data and Comments
Reported size (kB)

1.8 (longest cDNA)

(Kasravi et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
SCOT-PA
FBpp0072723
54.9
516
8.61
NP_647683
AAF47600
SCOT-PB
FBpp0072722
53.4
502
6.93
NP_728699
AAN11508
SCOT-PC
FBpp0305622
48.0
450
8.15
NP_001261301
AGB93996
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Homodimer.

(UniProt, Q9W058)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\SCOT using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\SCOT in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of SCOT
Transgenic constructs containing regulatory region of SCOT
Deletions and Duplications ( 0 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\OXCT1
15 of 15
Yes
Yes
Hsap\OXCT2
13 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Oxct1
15 of 15
Yes
Yes
Mmus\Oxct2b
12 of 15
No
Yes
Mmus\Oxct2a
10 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
Rnor\Oxct1
11 of 13
Yes
Yes
Rnor\Oxct2a
7 of 13
No
Yes
Rnor\LOC100361036
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\oxct1
3 of 12
Yes
Yes
Danio rerio (Zebrafish) (3)
Drer\oxct1a
12 of 15
Yes
Yes
Drer\oxct1b
10 of 15
No
Yes
Drer\LOC797311
2 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\C05C10.3
14 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091905XI )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00004427
Drosophila simulans
Dsim\GD13661
Drosophila sechellia
Dsec\GM14463
Drosophila erecta
Dere\GG14840
Drosophila yakuba
Dyak\GE21204
Drosophila ananassae
Dana\GF24850
Drosophila pseudoobscura pseudoobscura
Dpse\GA10977
Drosophila persimilis
Dper\GL22911
Drosophila willistoni
Dwil\GK12531
Drosophila virilis
Dvir\GJ11929
Drosophila mojavensis
Dmoj\GI13155
Drosophila grimshawi
Dgri\GH14994
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504JF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17012456
Glossina morsitans
Tsetse fly
Gmor\GMOY002902
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_05910
Mayetiola destructor
Hessian fly
Mdes\Mdes000933
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011137
Anopheles darlingi
American malaria mosquito
Adar\ADAC003963
Anopheles gambiae
Malaria mosquito
Agam\Succinyl_COA_transferase
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ011934
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01SV )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA000398
Danaus plexippus
Monarch butterfly
Dple\DPOGS200923-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL002519
Apis florea
Little honeybee
Aflo\2651923348
Apis mellifera
Western honey bee
Amel\LOC409157
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22357
Bombus terrestris
Buff-tailed bumblebee
Bter\15208057
Linepithema humile
Argentine ant
Lhum\LH11148
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002784
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000331
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_01630
Tribolium castaneum
Red flour beetle
Tcas\TC011712
Pediculus humanus
Human body louse
Phum\PHUM307460
Rhodnius prolixus
Kissing bug
Rpro\RPRC007961
Cimex lectularius
Bed bug
Clec\CLEC008423
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001153
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_09992
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01QO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR015399
Ixodes scapularis
Black-legged tick
Isca\ISCW002707
Ixodes scapularis
Black-legged tick
Isca\ISCW015960
Stegodyphus mimosarum
African social velvet spider
Smim\KFM70736.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM59368.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur10g05300
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_300886
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_220335
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0D3X )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_026678gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Gipc1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Oxct1_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Oxct1
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000009648
Ciona intestinalis
Vase tunicate
Cint\LOC100180566
Gallus gallus
Domestic chicken
Ggal\GIPC2
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000028029
Gallus gallus
Domestic chicken
Ggal\OXCT1
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    OXCT1; 3-oxoacid CoA-transferase 1
    15 of 15
    601424
      OXCT2; 3-oxoacid CoA-transferase 2
      13 of 15
      610289
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Interactions
          Summary of Physical Interactions
          esyN Network Diagram
          Interactions Browser
          Summary of Genetic Interactions
          esyN Network Diagram
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          External Data
          Subunit Structure (UniProtKB)
          Homodimer.
          (UniProt, Q9W058 )
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Pathways
          Signaling Pathways (FlyBase)
          Metabolic Pathways
          External Data
          Linkouts
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          3L
          Recombination map

          3-1.0

          Cytogenetic map
          62B11-62B12
          Sequence location
          3L:1,963,143..1,966,805 [+]
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          62B11-62B12
          Limits computationally determined from genome sequence between P{PZ}dlt04276 and P{PZ}slsrL182
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          Experimentally Determined Recombination Data
          Location

          3-1.0

          (FlyBase, 2016)

          3-3.4

          (Comeron et al., 2012)
          Left of (cM)
          Right of (cM)
          Notes
          Stocks and Reagents
          Stocks (8)
          Genomic Clones (15)
           

          Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

          cDNA Clones (46)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
          Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: SCOT CG1140

          (Zhang et al., 2013)
          Source for database merge of

          Source for merge of: CG1140 3-oxoacid-CoA-transferase

          (FlyBase, 1996-)

          Source for merge of: CG1140 BcDNA:RH54340

          (FlyBase, 1996-)
          Additional comments

          Source for merge of CG1140 BcDNA:RH54340 was a shared cDNA ( date:030728 ).

          (FlyBase, 1996-)
          Other Comments

          Identification: transcription unit identified during molecular analysis of the mu2 genomic region.

          (Kasravi et al., 1999)
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 54 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DPiM - Drosophila Protein interaction map
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Synonyms and Secondary IDs (11)
          Reported As
          Symbol Synonym
          BcDNA:RH54340
          CG1140
          (Aradska et al., 2015, Cammarato et al., 2011, Ni et al., 2010.12.1, Teleman et al., 2008, Rehmsmeier et al., 2004)
          CT1116
          (Ceriani et al., 2002)
          DmSCOT
          (Zhang et al., 2013)
          SCOT
          (Transgenic RNAi Project members, 2017-)
          cDNA B
          (Kasravi et al., 1999)
          Name Synonyms
          3-oxoacid CoA-transferase
          (Teleman et al., 2008, Kasravi et al., 1999)
          3-oxoacid-CoA transferase
          (Celotto et al., 2011)
          3-oxoacid-CoA-transferase
          Succinyl- CoA:3-ketoacid CoA transferase
          (Zhang et al., 2013)
          citric acid synthetase
          (Kasravi et al., 1999)
          Secondary FlyBase IDs
          • FBgn0027933
          • FBgn0064797
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (44)