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Gene Dmel\pfk

General Information
Symbol	Dmel\pfk	Species	D. melanogaster
Name	piefke	Annotation symbol	CG15812
Feature type	protein_coding_gene	FlyBase ID	FBgn0035405
Gene Model Status	Current	Stock availability	7 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	63B7-63B8	Sequence location	3L:3,159,991..3,162,019 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene piefke is referred to in FlyBase by the symbol Dmel\pfk (CG15812, FBgn0035405). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: DNA binding. There is experimental evidence that it is involved in the biological process: neurogenesis. 8 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: BTB/POZ; BTB/POZ fold; BTB/POZ-like; DNA binding HTH domain, Psq-type; Homeodomain-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1622998_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of pfk. Gene sequence location is 3L:3159991..3162019.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 63B7-63B8   Limits computationally determined from genome sequence between P{PZ}l(3)0680306803 and P{PZ}kst01318
Experimentally Determined Cytological Location
Cytogenetic map Notes References 63B-63B     (Schwendemann et al., 2001)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\pfk for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) pfk-RA FBtr0073027  NM_079173   1958   518 pfk-RB FBtr0073028  NM_167991   1913   512
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein pfk-PA   FBpp0072891   57.8   518   4.97     NP_523897   AAF47724 pfk-PB   FBpp0072892   57.0   512   4.87     NP_728817   AAN11534
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • BTB/POZ fold (IPR011333) BTB/POZ-like (IPR000210) DNA binding HTH domain, Psq-type (IPR007889) BTB/POZ (IPR013069) Homeodomain-like (IPR009057)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390797   AA392009   AA392431   AA439678   AA439827   AA540755   AA951224   AA978835   AA979674   AE014296 AAF47724   AE014296 AAN11534   AI108407   AI295517   AI519119   AI532678   AI947068   AY113532 AAM29537   BI240619   BI373538   BI636680   BT023904   BT044338   CK604883   CO156707   CO278639   CO329155   EC233211   EC244087   EC261246   EL874485   EL874559   EL874630   EV592099
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q86BP7 Q9VZU3 Q8MYX0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | intense & ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   central brain anlage   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 9 -- 10   central brain primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) gonad   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Schwendemann et al., 2001) polytene chromosome inferred from direct assay (Schwendemann et al., 2001)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    158.525 Larval Midgut    44.4 Larval Hindgut    53.4 Larval Malpighian Tubules    39.4 Larval Fat Body    29.4 Larval Salivary Gland    90.2 Larval Trachea    67.775 Larval Carcass    54.075 Adult Head    62.8 Adult Eye    44.475 Adult Brain    102.2 Adult Thoracic-Abdominal Ganglion    76.6 Adult Crop    60.5 Adult Midgut    53.9 Adult Hindgut    50.8 Adult Malpighian Tubules    50.6 Adult Fat Body    43.8 Adult Salivary Gland    49.3 Adult Heart    33.375 Adult VirginFemale Spermatheca    54.8 Adult InseminatedFemale Spermatheca    63.4 Adult Ovary    300.8 Adult Testis    71.1 Adult Male Accessory Gland    65.3 Adult Carcass    49.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    158.525 Larval Midgut    44.4 Larval Hindgut    53.4 Larval Malpighian Tubules    39.4 Larval Fat Body    29.4 Larval Salivary Gland    90.2 Larval Trachea    67.775 Larval Carcass    54.075 Adult Head    62.8 Adult Eye    44.475 Adult Brain    102.2 Adult Thoracic-Abdominal Ganglion    76.6 Adult Crop    60.5 Adult Midgut    53.9 Adult Hindgut    50.8 Adult Malpighian Tubules    50.6 Adult Fat Body    43.8 Adult Salivary Gland    49.3 Adult Heart    33.375 Adult VirginFemale Spermatheca    54.8 Adult InseminatedFemale Spermatheca    63.4 Adult Ovary    300.8 Adult Testis    71.1 Adult Male Accessory Gland    65.3 Adult Carcass    49.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    158.525 Larval Midgut    44.4 Larval Hindgut    53.4 Larval Malpighian Tubules    39.4 Larval Fat Body    29.4 Larval Salivary Gland    90.2 Larval Trachea    67.775 Larval Carcass    54.075 Adult Head    62.8 Adult Eye    44.475 Adult Brain    102.2 Adult Thoracic-Abdominal Ganglion    76.6 Adult Crop    60.5 Adult Midgut    53.9 Adult Hindgut    50.8 Adult Malpighian Tubules    50.6 Adult Fat Body    43.8 Adult Salivary Gland    49.3 Adult Heart    33.375 Adult VirginFemale Spermatheca    54.8 Adult InseminatedFemale Spermatheca    63.4 Adult Ovary    300.8 Adult Testis    71.1 Adult Male Accessory Gland    65.3 Adult Carcass    49.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    158.525 Larval Midgut    44.4 Larval Hindgut    53.4 Larval Malpighian Tubules    39.4 Larval Fat Body    29.4 Larval Salivary Gland    90.2 Larval Trachea    67.775 Larval Carcass    54.075 Adult Head    62.8 Adult Eye    44.475 Adult Brain    102.2 Adult Thoracic-Abdominal Ganglion    76.6 Adult Crop    60.5 Adult Midgut    53.9 Adult Hindgut    50.8 Adult Malpighian Tubules    50.6 Adult Fat Body    43.8 Adult Salivary Gland    49.3 Adult Heart    33.375 Adult VirginFemale Spermatheca    54.8 Adult InseminatedFemale Spermatheca    63.4 Adult Ovary    300.8 Adult Testis    71.1 Adult Male Accessory Gland    65.3 Adult Carcass    49.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    158.525 Larval Midgut    44.4 Larval Hindgut    53.4 Larval Malpighian Tubules    39.4 Larval Fat Body    29.4 Larval Salivary Gland    90.2 Larval Trachea    67.775 Larval Carcass    54.075 Adult Head    62.8 Adult Eye    44.475 Adult Brain    102.2 Adult Thoracic-Abdominal Ganglion    76.6 Adult Crop    60.5 Adult Midgut    53.9 Adult Hindgut    50.8 Adult Malpighian Tubules    50.6 Adult Fat Body    43.8 Adult Salivary Gland    49.3 Adult Heart    33.375 Adult VirginFemale Spermatheca    54.8 Adult InseminatedFemale Spermatheca    63.4 Adult Ovary    300.8 Adult Testis    71.1 Adult Male Accessory Gland    65.3 Adult Carcass    49.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    158.525 Larval Midgut    44.4 Larval Hindgut    53.4 Larval Malpighian Tubules    39.4 Larval Fat Body    29.4 Larval Salivary Gland    90.2 Larval Trachea    67.775 Larval Carcass    54.075 Adult Head    62.8 Adult Eye    44.475 Adult Brain    102.2 Adult Thoracic-Abdominal Ganglion    76.6 Adult Crop    60.5 Adult Midgut    53.9 Adult Hindgut    50.8 Adult Malpighian Tubules    50.6 Adult Fat Body    43.8 Adult Salivary Gland    49.3 Adult Heart    33.375 Adult VirginFemale Spermatheca    54.8 Adult InseminatedFemale Spermatheca    63.4 Adult Ovary    300.8 Adult Testis    71.1 Adult Male Accessory Gland    65.3 Adult Carcass    49.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    158.525 Larval Midgut    44.4 Larval Hindgut    53.4 Larval Malpighian Tubules    39.4 Larval Fat Body    29.4 Larval Salivary Gland    90.2 Larval Trachea    67.775 Larval Carcass    54.075 Adult Head    62.8 Adult Eye    44.475 Adult Brain    102.2 Adult Thoracic-Abdominal Ganglion    76.6 Adult Crop    60.5 Adult Midgut    53.9 Adult Hindgut    50.8 Adult Malpighian Tubules    50.6 Adult Fat Body    43.8 Adult Salivary Gland    49.3 Adult Heart    33.375 Adult VirginFemale Spermatheca    54.8 Adult InseminatedFemale Spermatheca    63.4 Adult Ovary    300.8 Adult Testis    71.1 Adult Male Accessory Gland    65.3 Adult Carcass    49.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    158.525 Larval Midgut    44.4 Larval Hindgut    53.4 Larval Malpighian Tubules    39.4 Larval Fat Body    29.4 Larval Salivary Gland    90.2 Larval Trachea    67.775 Larval Carcass    54.075 Adult Head    62.8 Adult Eye    44.475 Adult Brain    102.2 Adult Thoracic-Abdominal Ganglion    76.6 Adult Crop    60.5 Adult Midgut    53.9 Adult Hindgut    50.8 Adult Malpighian Tubules    50.6 Adult Fat Body    43.8 Adult Salivary Gland    49.3 Adult Heart    33.375 Adult VirginFemale Spermatheca    54.8 Adult InseminatedFemale Spermatheca    63.4 Adult Ovary    300.8 Adult Testis    71.1 Adult Male Accessory Gland    65.3 Adult Carcass    49.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    62.8   NA  Eye    44.475   NA  Brain    102.2   158.525  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    76.6   NA  Crop    60.5   44.4  Midgut    53.9   53.4  Hindgut    50.8   39.4  Malpighian Tubules    50.6   29.4  Fat Body    43.8   90.2  Salivary Gland    49.3   NA  Heart    33.375   67.775  Trachea    NA   NA  VirginFemale Spermatheca    54.8   NA  InseminatedFemale Spermatheca    63.4   NA  Ovary    300.8   NA  Testis    71.1   NA  Male Accessory Gland    65.3   54.075  Carcass    49.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    12 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    28 ovary, mated 4-day female    27 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    12 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female  (28) ovary, mated 4-day female  (27) testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    12 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    28 ovary, mated 4-day female    27 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    12 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    28 ovary, mated 4-day female    27 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    12 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    28 ovary, mated 4-day female    27 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    12 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female  28 ovary, mated 4-day female  27 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    12 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    28 ovary, mated 4-day female    27 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    12 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    28 ovary, mated 4-day female    27 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    60 embryo 02-04hr    49 embryo 04-06hr    36 embryo 06-08hr    48 embryo 08-10hr    45 embryo 10-12hr    43 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    20 embryo 18-20hr    15 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (60) embryo 02-04hr  (49) embryo 04-06hr  (36) embryo 06-08hr  (48) embryo 08-10hr  (45) embryo 10-12hr  (43) embryo 12-14hr  26 embryo 14-16hr    25 embryo 16-18hr    20 embryo 18-20hr    15 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    16 adult female 05day  (27) adult female 30day  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    60 embryo 02-04hr    49 embryo 04-06hr    36 embryo 06-08hr    48 embryo 08-10hr    45 embryo 10-12hr    43 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    20 embryo 18-20hr    15 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    60 embryo 02-04hr    49 embryo 04-06hr    36 embryo 06-08hr    48 embryo 08-10hr    45 embryo 10-12hr    43 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    20 embryo 18-20hr    15 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    60 embryo 02-04hr    49 embryo 04-06hr    36 embryo 06-08hr    48 embryo 08-10hr    45 embryo 10-12hr    43 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    20 embryo 18-20hr    15 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (60) embryo 02-04hr  (49) embryo 04-06hr  (36) embryo 06-08hr  (48) embryo 08-10hr  (45) embryo 10-12hr  (43) embryo 12-14hr  26 embryo 14-16hr    25 embryo 16-18hr    20 embryo 18-20hr    15 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    16 adult female 05day  27 adult female 30day  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    60 embryo 02-04hr    49 embryo 04-06hr    36 embryo 06-08hr    48 embryo 08-10hr    45 embryo 10-12hr    43 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    20 embryo 18-20hr    15 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    60 embryo 02-04hr    49 embryo 04-06hr    36 embryo 06-08hr    48 embryo 08-10hr    45 embryo 10-12hr    43 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    20 embryo 18-20hr    15 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    16 embryonic 1182-4H    11 embryonic GM2    11 embryonic Kc167    17 embryonic S1    14 embryonic S3    8 leg disc CME L1    14 wing disc CME-W2    8 wing disc ML-DmD8    12 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    18 wing disc ML-DmD32    7 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    9 ovary fGS/OSS    18 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (33) Schneider line 2 Sg4    16 embryonic 1182-4H    11 embryonic GM2    11 embryonic Kc167    17 embryonic S1    14 embryonic S3    8 leg disc CME L1    14 wing disc CME-W2    8 wing disc ML-DmD8    12 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    18 wing disc ML-DmD32    7 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    9 ovary fGS/OSS    18 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    16 embryonic 1182-4H    11 embryonic GM2    11 embryonic Kc167    17 embryonic S1    14 embryonic S3    8 leg disc CME L1    14 wing disc CME-W2    8 wing disc ML-DmD8    12 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    18 wing disc ML-DmD32    7 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    9 ovary fGS/OSS    18 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    16 embryonic 1182-4H    11 embryonic GM2    11 embryonic Kc167    17 embryonic S1    14 embryonic S3    8 leg disc CME L1    14 wing disc CME-W2    8 wing disc ML-DmD8    12 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    18 wing disc ML-DmD32    7 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    9 ovary fGS/OSS    18 ovary OSC    13 ovary OSS    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    16 embryonic 1182-4H    11 embryonic GM2    11 embryonic Kc167    17 embryonic S1    14 embryonic S3    8 leg disc CME L1    14 wing disc CME-W2    8 wing disc ML-DmD8    12 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    18 wing disc ML-DmD32    7 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    9 ovary fGS/OSS    18 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (33) Schneider line 2 Sg4    16 embryonic 1182-4H    11 embryonic GM2    11 embryonic Kc167    17 embryonic S1    14 embryonic S3    8 leg disc CME L1    14 wing disc CME-W2    8 wing disc ML-DmD8    12 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    18 wing disc ML-DmD32    7 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    9 ovary fGS/OSS    18 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    16 embryonic 1182-4H    11 embryonic GM2    11 embryonic Kc167    17 embryonic S1    14 embryonic S3    8 leg disc CME L1    14 wing disc CME-W2    8 wing disc ML-DmD8    12 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    18 wing disc ML-DmD32    7 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    9 ovary fGS/OSS    18 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    16 embryonic 1182-4H    11 embryonic GM2    11 embryonic Kc167    17 embryonic S1    14 embryonic S3    8 leg disc CME L1    14 wing disc CME-W2    8 wing disc ML-DmD8    12 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    18 wing disc ML-DmD32    7 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    9 ovary fGS/OSS    18 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3  26 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3  26 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • FI07618 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035405 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG15812-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal pfkGD5047 viable, with Scer\GAL4pnr-MD237 pfkGD5047 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 pfkGD5047 neuroanatomy defective | larval stage pfkGD5047 visible, with Scer\GAL4pnr-MD237 pfkGD5047 Phenotype manifest in Allele ganglion mother cell | larval stage pfkGD5047 mesothoracic tergum, with Scer\GAL4pnr-MD237 pfkGD5047 trichogen cell, with Scer\GAL4pnr-MD237 pfkGD5047
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of pfk Class Mutagen Stocks Known lesion pfkG18480 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of pfk Class Mutagen Stocks Known lesion pfkGD5047 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes pfkGLV21023 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pfkJF01391 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pfkJF02204 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pfkKK111556 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pfkNIG.15812R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes pfkt17.7 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5047} NA P{KK111556} NA P{NIG.15812R} NA P{TRiP.GLV21023} NA P{TRiP.JF01391} NA P{TRiP.JF02204} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}pfkG18480 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Schwendemann et al., 2001) polytene chromosome inferred from direct assay (Schwendemann et al., 2001)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR007889, InterPro:IPR011526 (FlyBase Curators et al., 2004-) non-traceable author statement (Lehmann, 2002.7.1)
Biological Process
CV term Evidence References
chromatin remodeling non-traceable author statement (Schwendemann et al., 2001)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions pfk allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63877 DroID - A comprehensive database of gene and protein interactions. • FBgn0035405 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38379
Orthologs
OrthoDB Orthologs (17) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6S4Q23 Dipteran inclusive ortholog search EOG6BG94N Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6S4Q23)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\pfk     Drosophila simulans   Dsim\GD13730 Y   Drosophila sechellia   Dsec\GM14537 Y   Drosophila erecta   Dere\GG14908 Y   Drosophila yakuba   Dyak\GE20362 Y   Drosophila ananassae   Dana\GF24271 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA13965 Y   Drosophila persimilis   Dper\GL20787     Drosophila persimilis   Dper\GL20786     Drosophila willistoni   Dwil\GK17761 Y   Drosophila virilis   Dvir\GJ13141 Y   Drosophila mojavensis   Dmoj\GI12997 Y   Drosophila grimshawi   Dgri\GH15392 Y
Orthologs in non-Drosophila Dipterans (EOG6BG94N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010842   Anopheles gambiae Malaria mosquito  Agam\AGAP006458   Anopheles gambiae Malaria mosquito  Agam\AGAP006457   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013500
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD13730 Drosophila sechellia Dsec\GM14537 Drosophila erecta Dere\GG14908 Drosophila yakuba Dyak\GE20362 Drosophila ananassae Dana\GF24271 Drosophila pseudoobscura pseudoobscura Dpse\GA13965 Drosophila willistoni Dwil\GK17761 Drosophila virilis Dvir\GJ13141 Drosophila mojavensis Dmoj\GI12997 Drosophila grimshawi Dgri\GH15392
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	31607 y1 v1; P{TRiP.JF01391}attP2 31914 y1 v1; P{TRiP.JF02204}attP2 27444 y1 w*; P{EP}pfkG18480 35658 y1 sc* v1; P{TRiP.GLV21023}attP2
VDRC	v107517 P{KK111556}VIE-260B v13361 w1118; P{GD5047}v13361 v14020 w1118; P{GD5047}v14020
Genomic Clones ( 1 )
	BACR48E19 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 30 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP14421 RE60854
Other clones	FI07618
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH07233 LD08856 LD10943 LD12813 LD14310 LD14503 LD20557 LD22127 LD31577 LD34387 LD38539 LP09176 RE37503 SD04266 SD18458 UT01515
Other clones	18398 18724 528158 bs34f09 Dmel_neb_009693_0599_0576 EK130660 EK292130 EN08647 IP14521 IP14621 IP14721
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0035405 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38379
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: pfk CG15812 (Schwendemann, 2001.3.5)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079173 NM_167991 RefSeq (Proteins) • NP_523897 NP_728817 DDBJ / EMBL / GenBank • AA439827 AAF47724 AAM29537 AAN11534 AI947068 AY113532 BI373538 Entrez Gene - A searchable database of RefSeq genes. • 38379 UniProtKB/TrEMBL • Q8MYX0 Q9VZU3 Q86BP7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • BTB/POZ fold (IPR011333) BTB/POZ-like (IPR000210) DNA binding HTH domain, Psq-type (IPR007889) BTB/POZ (IPR013069) Homeodomain-like (IPR009057) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • FI07618
Linkouts
	BioGRID - A database of protein and genetic interactions • 63877 DroID - A comprehensive database of gene and protein interactions. • FBgn0035405 DRSC - Results from RNAi screens. • FBgn0035405 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035405 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG15812-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0035405 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38379 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38379 modMine - Data generated by the modENCODE project. • FBgn0035405
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG15812 (Chamoun et al., 2001, Lee and Treisman, 2001, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Ni et al., 2011.7.19) pfk (Japanese National Institute of Genetics, 2012.5.21)
Name Synonym	piefke (Schwendemann et al., 2001)
Secondary FlyBase IDs
References ( 27 )
Generate a list of	All references relating to this gene ( 27 )
List References by type	Research paper  ( 5 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...