FlyBase Gene Report: Dmel\RpL28

CYTOPLASMIC LARGE RIBOSOMAL PROTEINS -: Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytoplasm. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the large subunit is to bind tRNA during the synthesis of polypeptides. (Adapted from FBrf0205398).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\RpL28 or the JBrowse view of Dmel\RpL28 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RpL28-RA

FBtr0073097

NM_139534

547

144

RpL28-RB

FBtr0073095

NM_168004

887

144

RpL28-RD

FBtr0073098

NM_168005

656

144

RpL28-RE

FBtr0302300

NM_001169865

650

144

RpL28-RF

FBtr0302301

NM_001169866

624

144

RpL28-RG

FBtr0306567

NM_168006

851

144

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

RpL28-PA

FBpp0072959

16.0

144

11.74

NP_647791

AAN11546

RpL28-PB

FBpp0072957

16.0

144

11.74

NP_728839

AAF47742

RpL28-PD

FBpp0072960

16.0

144

11.74

NP_728840

AAN11547

RpL28-PE

FBpp0291506

16.0

144

11.74

NP_001163336

ACZ94608

RpL28-PF

FBpp0291507

16.0

144

11.74

NP_001163337

ACZ94609

RpL28-PG

FBpp0297522

16.0

144

11.74

NP_728841

AAF47743

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

144 aa isoforms: RpL28-PA, RpL28-PB, RpL28-PD, RpL28-PE, RpL28-PF, RpL28-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpL28 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Gene Ontology (4 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

structural constituent of ribosome

inferred from electronic annotation with InterPro:IPR002672

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

traceable author statement

(Marygold et al., 2007)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

cytoplasmic translation

traceable author statement

(Marygold et al., 2007)

translation

inferred from electronic annotation with InterPro:IPR002672

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

cytosolic large ribosomal subunit

inferred from biological aspect of ancestor with PANTHER:PTN000057380

(assigned by GO_Central )

(Gaudet et al., 2011)

traceable author statement

(Marygold et al., 2007)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

(Arbeitman et al., 2004)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

(Arbeitman et al., 2004)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RpL28 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RpL28

View exonic expression by tissue for Dmel\RpL28

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0035422

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG12740-RB;CG12740-RC

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12740

Flygut - An atlas of the Drosophila adult midgut

FBgn0035422

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RpL28

Transgenic constructs containing regulatory region of RpL28

Deletions and Duplications ( 2 )

Disrupted in

Df(3L)HR370

(Marygold et al., 2007)

Df(3L)M21

(Marygold et al., 2007)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

RpL28^GD7589

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpL28^GD17335

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

RpL28^GD7589

lethal - all die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpL28^GD17335

partially lethal, with Scer\GAL4^tin.CΔ4

RpL28^GD17335

partially lethal - majority die, with Scer\GAL4^pnr-MD237

RpL28^GD7589

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpL28^GD7589

viable, with Scer\GAL4^tin.CΔ4

RpL28^GD7589

Other Phenotypes

Allele

neuroanatomy defective | larval stage

RpL28^GD7589

some die during pupal stage, with Scer\GAL4^pnr-MD237

RpL28^GD7589

visible, with Scer\GAL4^pnr-MD237

RpL28^GD7589

Phenotype manifest in

Allele

ganglion mother cell | larval stage

RpL28^GD7589

larval neuroblast | larval stage

RpL28^GD7589

mesothoracic tergum, with Scer\GAL4^pnr-MD237

RpL28^GD7589

trichogen cell, with Scer\GAL4^pnr-MD237

RpL28^GD7589

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RPL28

13 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rpl28

13 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\Rpl28

13 of 13

Yes

Rnor\LOC100360619

4 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\rpl28

9 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\rpl28

10 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\rpl-28

9 of 15

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\AT2G19730

8 of 9

Yes

Atha\AT4G29410

8 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\rpl44

12 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190IXS )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\RpL28

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11782

Drosophila persimilis

Dper\GL21281

Drosophila willistoni

Dwil\GK16691

Drosophila virilis

Dvir\GJ15527

Drosophila mojavensis

Dmoj\GI12761

Drosophila grimshawi

Dgri\GH15706

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150FLK )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17012240

Glossina morsitans

Tsetse fly

Gmor\GMOY001520

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10746

Mayetiola destructor

Hessian fly

Mdes\Mdes001687

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008353

Anopheles darlingi

American malaria mosquito

Adar\ADAC005334

Anopheles gambiae

Malaria mosquito

Agam\Rpl28

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008421

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0JIS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\RpL28

Danaus plexippus

Monarch butterfly

Dple\DPOGS205446-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL014719

Apis florea

Little honeybee

Aflo\2774598836

Apis mellifera

Western honey bee

Amel\GB52512

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP19560

Bombus terrestris

Buff-tailed bumblebee

Bter\15202061

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002c1e

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000176

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07432

Pediculus humanus

Human body louse

Phum\PHUM501030

Rhodnius prolixus

Kissing bug

Rpro\RPRC012353

Rhodnius prolixus

Kissing bug

Rpro\RPRC012354

Cimex lectularius

Bed bug

Clec\CLEC000404

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006342

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_03301

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0JNN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR004886

Ixodes scapularis

Black-legged tick

Isca\ISCW002598

Stegodyphus mimosarum

African social velvet spider

Smim\KFM60036.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur01g09390

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_230702

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0W22 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rpl28

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RPL28; ribosomal protein L28

13 of 15

603638

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RpL28 - Dsor1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

RpL28 - Lkb1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpL28 - Myc

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpL28 - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpL28 - Pi3K92E

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpL28 - Rack1

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

RpL28 - RpL10

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL10Ab

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL13A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL14

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL18A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL19

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL22

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL23A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL30

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL31

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL32

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL34a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL35

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - RpL35A

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - RpL36

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL36A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL37A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL37a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL38

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL39

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - RpL4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL40

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - RpL41

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - RpL5

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL7A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpL9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpLP0

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpLP1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpLP2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS10a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS14a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS14b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS15Aa

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS16

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS19a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS20

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS25

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS28b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS30

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - RpS3A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS5a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - RpS9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpL28 - Thor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpL28 - eEF2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpL28 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpL28 - sta

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpL28 - vig2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RNA-protein

Physical Interaction

Assay

References

RpL28 - 18SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - 28SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - 2SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - 5.8SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpL28 - 5SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63893

DroID - A comprehensive database of gene and protein interactions.

FBgn0035422

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38397

MIST (protein-protein) - An integrated Molecular Interaction Database

RpL28

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-6

Cytogenetic map

63B14-63B14

Sequence location

3L:3,220,911..3,222,960 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63B14-63B14

Limits computationally determined from genome sequence between P{PZ}l(3)06803⁰⁶⁸⁰³ and P{PZ}kst⁰¹³¹⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-6

(FlyBase, 2016)

3-6.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Bloomington

36076

y¹ sc^* v¹ sev²¹; P{TRiP.GL00495}attP2

VDRC

v104021

P{KK107863}VIE-260B

v49124

w¹¹¹⁸; P{GD17335}v49124

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (460)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

RE52852

Other clones

MIP15344

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0035422

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0035422

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38397

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: RpL28 anon-WO0118547.321

(FlyBase, 1996-)

Additional comments

"RpL28" likely corresponds to "M(3)63B".

(Marygold et al., 2007)

Source for merge of RpL28 anon-WO0118547.321 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Haploinsufficient locus.

(Cook et al., 2012)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Likely Minute gene.

(Marygold et al., 2007)

Deletions removing RpL28 but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.

(Marygold et al., 2007)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Area matching Drosophila EST AA8033640. This EST forms a 859bp contig with ESTs AA803683 and AA803676 and has sequence similarity to Human Ribosomal L28 protein.

(Davies, 2001.3.15)

Identified with: GH04183 (BDGP-DGC) <up>FlyBase curator comment: EST subsequently found to be chimeric</up>.

(BDGP Project Members, 2000-)

EST GH04183 is chimeric; the 5' portion matches part of AE003468 on the opposite strand to CG17180, the 3' portion corresponds to part of RpL28.

(FlyBase, 1996-)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 59 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38397

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VZS5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

D1Z3A1

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0035422

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12740

Flygut - An atlas of the Drosophila adult midgut

FBgn0035422

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38397

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG12740

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

modMine - A data warehouse for the modENCODE project

FBgn0035422

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Linkouts

BioGRID - A database of protein and genetic interactions.

63893

DroID - A comprehensive database of gene and protein interactions.

FBgn0035422

DRSC - Results frm RNAi screens

FBgn0035422

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0035422

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG12740-RB;CG12740-RC

FlyMine - An integrated database for Drosophila genomics

FBgn0035422

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38397

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

MIST (protein-protein) - An integrated Molecular Interaction Database

RpL28

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

BcDNA:GH04183

CG12740

(Kolkhof et al., 2017, Schertel et al., 2013, Cammarato et al., 2011, Ni et al., 2011.7.19, Müller et al., 2010, Keleman et al., 2009.8.5, Goshima et al., 2007, Marygold et al., 2007, Stuart et al., 2007, Huang et al., 2005, Accardo et al., 2004, Arbeitman et al., 2004, Boutros et al., 2004, King et al., 2002.9.23)

GH04183p

(Jaspers et al., 2012)

L28

(Brogna et al., 2002)

RpL28

(Zolotarev et al., 2017, Lefebvre et al., 2016, Angrisani et al., 2015, Handler et al., 2013, Schertel et al., 2013, Vilmos et al., 2013, Cook et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Neumüller et al., 2011, Müller et al., 2010, Nie et al., 2009, Goshima et al., 2007, Marygold et al., 2007)

anon-WO0118547.321

chr3L:3205394..3205484

(Will et al., 2013)

Name Synonyms

60 S ribosomal protein L28

(Pflieger et al., 2008)

Ribosomal protein L28

(Cammarato et al., 2011, Ashburner and Marygold, 2004.7.2)

putativeribosomal L28e protein

(Accardo et al., 2004)

Secondary FlyBase IDs

FBgn0062095
FBgn0063278

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (61)

Report Sections

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