Gene Dmel\CG12016

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General Information

Symbol

Dmel\CG12016

Species

D. melanogaster

Name

Annotation symbol

CG12016

Feature type

protein_coding_gene

FlyBase ID

FBgn0035436

Gene Model Status

Current

Stock availability

3 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

63D1-63D1

Sequence location

3L:3,319,555..3,322,489 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG12016 (FBgn0035436). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: ribosylnicotinamide kinase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: nicotinamide nucleotide biosynthetic process. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Nicotinamide riboside kinase. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed during early pupal stages. This gene is annotated by FlyBase as a dicistronic gene, meaning that some or all of its transcripts encode two or more polypeptide-coding open reading frames (ORFs) , with each ORF assigned to a different gene. The distribution of RNA-Seq coverage data amongst the different encoded genes cannot be determined. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult thoracico-abdominal ganglion, adult crop, adult heart, larval/adult fat body, adult spermathecae, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1636920_s_at aligns to a genomic region shared by exons of 2 FlyBase-annotated genes; hence it is impossible to interpret the expression pattern and levels of CG12016 expression signal. See the GBrowse view of CG12016 to determine the overlapping gene(s). Gene sequence location is 3L:3319555..3322489.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63D1-63D1

Limits computationally determined from genome sequence between P{PZ}l(3)06803⁰⁶⁸⁰³ and P{PZ}kst⁰¹³¹⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG12016 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.45

gene_with_dicistronic_mRNA ; SO:0000722

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG12016-RA

FBtr0073063

NM_139548

2326

323

CG12016-RB

FBtr0073064

NM_168022

1995

323

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG12016-PA

FBpp0072927

37.9

323

7.90

NP_647805

AAF47759

CG12016-PB

FBpp0072928

37.9

323

7.90

NP_728865

AAN11559

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

P-loop containing nucleoside triphosphate hydrolase (IPR027417)

Nicotinamide riboside kinase (IPR026681)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VZR0

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint & ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint & ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint & ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult thoracico-abdominal ganglion, adult crop, adult heart, larval/adult fat body, adult spermathecae, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		397.925
Larval Midgut		126.5
Larval Hindgut		234.6
Larval Malpighian Tubules		147.2
Larval Fat Body		1618.2
Larval Salivary Gland		245.3
Larval Trachea		196.825
Larval Carcass		120.7
Adult Head		718
Adult Eye		481.425
Adult Brain		475.3
Adult Thoracic-Abdominal Ganglion		537.4
Adult Crop		785.1
Adult Midgut		187.9
Adult Hindgut		379.7
Adult Malpighian Tubules		181.5
Adult Fat Body		1239.6
Adult Salivary Gland		399.4
Adult Heart		723.475
Adult VirginFemale Spermatheca		2123.1
Adult InseminatedFemale Spermatheca		1201.7
Adult Ovary		477.5
Adult Testis		72.9
Adult Male Accessory Gland		278.6
Adult Carcass		634.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		397.925
Larval Midgut		126.5
Larval Hindgut		234.6
Larval Malpighian Tubules		147.2
Larval Fat Body		(1618.2)
Larval Salivary Gland		245.3
Larval Trachea		196.825
Larval Carcass		120.7
Adult Head		(718)
Adult Eye		481.425
Adult Brain		475.3
Adult Thoracic-Abdominal Ganglion		537.4
Adult Crop		(785.1)
Adult Midgut		187.9
Adult Hindgut		379.7
Adult Malpighian Tubules		181.5
Adult Fat Body		(1239.6)
Adult Salivary Gland		399.4
Adult Heart		(723.475)
Adult VirginFemale Spermatheca		(2123.1)
Adult InseminatedFemale Spermatheca		(1201.7)
Adult Ovary		477.5
Adult Testis		72.9
Adult Male Accessory Gland		278.6
Adult Carcass		(634.5)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		397.925
Larval Midgut		126.5
Larval Hindgut		234.6
Larval Malpighian Tubules		147.2
Larval Fat Body		(1618.2)
Larval Salivary Gland		245.3
Larval Trachea		196.825
Larval Carcass		120.7
Adult Head		718
Adult Eye		481.425
Adult Brain		475.3
Adult Thoracic-Abdominal Ganglion		537.4
Adult Crop		785.1
Adult Midgut		187.9
Adult Hindgut		379.7
Adult Malpighian Tubules		181.5
Adult Fat Body		(1239.6)
Adult Salivary Gland		399.4
Adult Heart		723.475
Adult VirginFemale Spermatheca		(2123.1)
Adult InseminatedFemale Spermatheca		(1201.7)
Adult Ovary		477.5
Adult Testis		72.9
Adult Male Accessory Gland		278.6
Adult Carcass		634.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		397.925
Larval Midgut		126.5
Larval Hindgut		234.6
Larval Malpighian Tubules		147.2
Larval Fat Body		1618.2
Larval Salivary Gland		245.3
Larval Trachea		196.825
Larval Carcass		120.7
Adult Head		718
Adult Eye		481.425
Adult Brain		475.3
Adult Thoracic-Abdominal Ganglion		537.4
Adult Crop		785.1
Adult Midgut		187.9
Adult Hindgut		379.7
Adult Malpighian Tubules		181.5
Adult Fat Body		1239.6
Adult Salivary Gland		399.4
Adult Heart		723.475
Adult VirginFemale Spermatheca		2123.1
Adult InseminatedFemale Spermatheca		1201.7
Adult Ovary		477.5
Adult Testis		72.9
Adult Male Accessory Gland		278.6
Adult Carcass		634.5
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		397.925
Larval Midgut		126.5
Larval Hindgut		234.6
Larval Malpighian Tubules		147.2
Larval Fat Body		1618.2
Larval Salivary Gland		245.3
Larval Trachea		196.825
Larval Carcass		120.7
Adult Head		718
Adult Eye		481.425
Adult Brain		475.3
Adult Thoracic-Abdominal Ganglion		537.4
Adult Crop		785.1
Adult Midgut		187.9
Adult Hindgut		379.7
Adult Malpighian Tubules		181.5
Adult Fat Body		1239.6
Adult Salivary Gland		399.4
Adult Heart		723.475
Adult VirginFemale Spermatheca		2123.1
Adult InseminatedFemale Spermatheca		1201.7
Adult Ovary		477.5
Adult Testis		72.9
Adult Male Accessory Gland		278.6
Adult Carcass		634.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		397.925
Larval Midgut		126.5
Larval Hindgut		234.6
Larval Malpighian Tubules		147.2
Larval Fat Body		(1618.2)
Larval Salivary Gland		245.3
Larval Trachea		196.825
Larval Carcass		120.7
Adult Head		718
Adult Eye		481.425
Adult Brain		475.3
Adult Thoracic-Abdominal Ganglion		537.4
Adult Crop		785.1
Adult Midgut		187.9
Adult Hindgut		379.7
Adult Malpighian Tubules		181.5
Adult Fat Body		(1239.6)
Adult Salivary Gland		399.4
Adult Heart		723.475
Adult VirginFemale Spermatheca		(2123.1)
Adult InseminatedFemale Spermatheca		(1201.7)
Adult Ovary		477.5
Adult Testis		72.9
Adult Male Accessory Gland		278.6
Adult Carcass		634.5
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		397.925
Larval Midgut		126.5
Larval Hindgut		234.6
Larval Malpighian Tubules		147.2
Larval Fat Body		1618.2
Larval Salivary Gland		245.3
Larval Trachea		196.825
Larval Carcass		120.7
Adult Head		718
Adult Eye		481.425
Adult Brain		475.3
Adult Thoracic-Abdominal Ganglion		537.4
Adult Crop		785.1
Adult Midgut		187.9
Adult Hindgut		379.7
Adult Malpighian Tubules		181.5
Adult Fat Body		1239.6
Adult Salivary Gland		399.4
Adult Heart		723.475
Adult VirginFemale Spermatheca		(2123.1)
Adult InseminatedFemale Spermatheca		1201.7
Adult Ovary		477.5
Adult Testis		72.9
Adult Male Accessory Gland		278.6
Adult Carcass		634.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		397.925
Larval Midgut		126.5
Larval Hindgut		234.6
Larval Malpighian Tubules		147.2
Larval Fat Body		1618.2
Larval Salivary Gland		245.3
Larval Trachea		196.825
Larval Carcass		120.7
Adult Head		718
Adult Eye		481.425
Adult Brain		475.3
Adult Thoracic-Abdominal Ganglion		537.4
Adult Crop		785.1
Adult Midgut		187.9
Adult Hindgut		379.7
Adult Malpighian Tubules		181.5
Adult Fat Body		1239.6
Adult Salivary Gland		399.4
Adult Heart		723.475
Adult VirginFemale Spermatheca		2123.1
Adult InseminatedFemale Spermatheca		1201.7
Adult Ovary		477.5
Adult Testis		72.9
Adult Male Accessory Gland		278.6
Adult Carcass		634.5
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	718
NA	Eye	481.425
NA	Brain	475.3
397.925	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	537.4
NA	Crop	785.1
126.5	Midgut	187.9
234.6	Hindgut	379.7
147.2	Malpighian Tubules	181.5
1618.2	Fat Body	1239.6
245.3	Salivary Gland	399.4
NA	Heart	723.475
196.825	Trachea	NA
NA	VirginFemale Spermatheca	2123.1
NA	InseminatedFemale Spermatheca	1201.7
NA	Ovary	477.5
NA	Testis	72.9
NA	Male Accessory Gland	278.6
120.7	Carcass	634.5

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		19
central nervous system, pupae P8		10
head, virgin 1-day female		34
head, virgin 4-day female		67
head, virgin 20-day female		61
head, mated 1-day female		43
head, mated 4-day female		27
head, mated 20-day female		47
head, mated 1-day male		71
head, mated 4-day male		56
head, mated 20-day male		73
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		47
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		9
digestive system, 4-day adult		10
digestive system, 20-day adult		11
fat body, larvae L3 wandering		43
fat body, white prepupae		447
fat body, pupae P8		40
carcass, larvae L3 wandering		15
carcass, 1-day adult		29
carcass, 4-day adult		27
carcass, 20-day adult		46
ovary, virgin 4-day female		30
ovary, mated 4-day female		29
testis, mated 4-day male		33
accessory gland, mated 4-day male		10
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		19
central nervous system, pupae P8		10
head, virgin 1-day female		(34)
head, virgin 4-day female		(67)
head, virgin 20-day female		(61)
head, mated 1-day female		(43)
head, mated 4-day female		(27)
head, mated 20-day female		(47)
head, mated 1-day male		(71)
head, mated 4-day male		(56)
head, mated 20-day male		(73)
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		(47)
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		9
digestive system, 4-day adult		10
digestive system, 20-day adult		11
fat body, larvae L3 wandering		(43)
fat body, white prepupae		(447)
fat body, pupae P8		(40)
carcass, larvae L3 wandering		15
carcass, 1-day adult		(29)
carcass, 4-day adult		(27)
carcass, 20-day adult		(46)
ovary, virgin 4-day female		(30)
ovary, mated 4-day female		(29)
testis, mated 4-day male		(33)
accessory gland, mated 4-day male		10
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		19
central nervous system, pupae P8		10
head, virgin 1-day female		34
head, virgin 4-day female		67
head, virgin 20-day female		61
head, mated 1-day female		43
head, mated 4-day female		27
head, mated 20-day female		47
head, mated 1-day male		71
head, mated 4-day male		56
head, mated 20-day male		73
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		47
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		9
digestive system, 4-day adult		10
digestive system, 20-day adult		11
fat body, larvae L3 wandering		43
fat body, white prepupae		(447)
fat body, pupae P8		40
carcass, larvae L3 wandering		15
carcass, 1-day adult		29
carcass, 4-day adult		27
carcass, 20-day adult		46
ovary, virgin 4-day female		30
ovary, mated 4-day female		29
testis, mated 4-day male		33
accessory gland, mated 4-day male		10
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		19
central nervous system, pupae P8		10
head, virgin 1-day female		34
head, virgin 4-day female		67
head, virgin 20-day female		61
head, mated 1-day female		43
head, mated 4-day female		27
head, mated 20-day female		47
head, mated 1-day male		71
head, mated 4-day male		56
head, mated 20-day male		73
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		47
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		9
digestive system, 4-day adult		10
digestive system, 20-day adult		11
fat body, larvae L3 wandering		43
fat body, white prepupae		447
fat body, pupae P8		40
carcass, larvae L3 wandering		15
carcass, 1-day adult		29
carcass, 4-day adult		27
carcass, 20-day adult		46
ovary, virgin 4-day female		30
ovary, mated 4-day female		29
testis, mated 4-day male		33
accessory gland, mated 4-day male		10
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		19
central nervous system, pupae P8		10
head, virgin 1-day female		34
head, virgin 4-day female		67
head, virgin 20-day female		61
head, mated 1-day female		43
head, mated 4-day female		27
head, mated 20-day female		47
head, mated 1-day male		71
head, mated 4-day male		56
head, mated 20-day male		73
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		47
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		9
digestive system, 4-day adult		10
digestive system, 20-day adult		11
fat body, larvae L3 wandering		43
fat body, white prepupae		447
fat body, pupae P8		40
carcass, larvae L3 wandering		15
carcass, 1-day adult		29
carcass, 4-day adult		27
carcass, 20-day adult		46
ovary, virgin 4-day female		30
ovary, mated 4-day female		29
testis, mated 4-day male		33
accessory gland, mated 4-day male		10
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		19
central nervous system, pupae P8		10
head, virgin 1-day female		(34)
head, virgin 4-day female		(67)
head, virgin 20-day female		(61)
head, mated 1-day female		(43)
head, mated 4-day female		27
head, mated 20-day female		(47)
head, mated 1-day male		(71)
head, mated 4-day male		(56)
head, mated 20-day male		(73)
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		(47)
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		9
digestive system, 4-day adult		10
digestive system, 20-day adult		11
fat body, larvae L3 wandering		(43)
fat body, white prepupae		(447)
fat body, pupae P8		(40)
carcass, larvae L3 wandering		15
carcass, 1-day adult		29
carcass, 4-day adult		27
carcass, 20-day adult		(46)
ovary, virgin 4-day female		30
ovary, mated 4-day female		29
testis, mated 4-day male		(33)
accessory gland, mated 4-day male		10
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		19
central nervous system, pupae P8		10
head, virgin 1-day female		34
head, virgin 4-day female		67
head, virgin 20-day female		61
head, mated 1-day female		43
head, mated 4-day female		27
head, mated 20-day female		47
head, mated 1-day male		71
head, mated 4-day male		56
head, mated 20-day male		73
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		47
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		9
digestive system, 4-day adult		10
digestive system, 20-day adult		11
fat body, larvae L3 wandering		43
fat body, white prepupae		(447)
fat body, pupae P8		40
carcass, larvae L3 wandering		15
carcass, 1-day adult		29
carcass, 4-day adult		27
carcass, 20-day adult		46
ovary, virgin 4-day female		30
ovary, mated 4-day female		29
testis, mated 4-day male		33
accessory gland, mated 4-day male		10
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		19
central nervous system, pupae P8		10
head, virgin 1-day female		34
head, virgin 4-day female		67
head, virgin 20-day female		61
head, mated 1-day female		43
head, mated 4-day female		27
head, mated 20-day female		47
head, mated 1-day male		71
head, mated 4-day male		56
head, mated 20-day male		73
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		47
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		9
digestive system, 4-day adult		10
digestive system, 20-day adult		11
fat body, larvae L3 wandering		43
fat body, white prepupae		447
fat body, pupae P8		40
carcass, larvae L3 wandering		15
carcass, 1-day adult		29
carcass, 4-day adult		27
carcass, 20-day adult		46
ovary, virgin 4-day female		30
ovary, mated 4-day female		29
testis, mated 4-day male		33
accessory gland, mated 4-day male		10
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed during early pupal stages. This gene is annotated by FlyBase as a dicistronic gene, meaning that some or all of its transcripts encode two or more polypeptide-coding open reading frames (ORFs) , with each ORF assigned to a different gene. The distribution of RNA-Seq coverage data amongst the different encoded genes cannot be determined.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		29
embryo 02-04hr		10
embryo 04-06hr		16
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		11
embryo 12-14hr		9
embryo 14-16hr		8
embryo 16-18hr		9
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		17
larva L1		20
larva L2		14
larva L3 12hr old		15
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		30
white prepupae new		60
white prepupae 12hr		38
white prepupae 24hr		19
pupae 2d postWPP		14
pupae 3d postWPP		9
pupae 4d postWPP		11
adult male 01day		29
adult male 05day		24
adult male 30day		24
adult female 01day		21
adult female 05day		24
adult female 30day		22
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(29)
embryo 02-04hr		10
embryo 04-06hr		16
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		11
embryo 12-14hr		9
embryo 14-16hr		8
embryo 16-18hr		9
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		17
larva L1		20
larva L2		14
larva L3 12hr old		15
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		(30)
white prepupae new		(60)
white prepupae 12hr		(38)
white prepupae 24hr		19
pupae 2d postWPP		14
pupae 3d postWPP		9
pupae 4d postWPP		11
adult male 01day		(29)
adult male 05day		24
adult male 30day		24
adult female 01day		21
adult female 05day		24
adult female 30day		22
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		29
embryo 02-04hr		10
embryo 04-06hr		16
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		11
embryo 12-14hr		9
embryo 14-16hr		8
embryo 16-18hr		9
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		17
larva L1		20
larva L2		14
larva L3 12hr old		15
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		30
white prepupae new		60
white prepupae 12hr		38
white prepupae 24hr		19
pupae 2d postWPP		14
pupae 3d postWPP		9
pupae 4d postWPP		11
adult male 01day		29
adult male 05day		24
adult male 30day		24
adult female 01day		21
adult female 05day		24
adult female 30day		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		29
embryo 02-04hr		10
embryo 04-06hr		16
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		11
embryo 12-14hr		9
embryo 14-16hr		8
embryo 16-18hr		9
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		17
larva L1		20
larva L2		14
larva L3 12hr old		15
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		30
white prepupae new		60
white prepupae 12hr		38
white prepupae 24hr		19
pupae 2d postWPP		14
pupae 3d postWPP		9
pupae 4d postWPP		11
adult male 01day		29
adult male 05day		24
adult male 30day		24
adult female 01day		21
adult female 05day		24
adult female 30day		22
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		29
embryo 02-04hr		10
embryo 04-06hr		16
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		11
embryo 12-14hr		9
embryo 14-16hr		8
embryo 16-18hr		9
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		17
larva L1		20
larva L2		14
larva L3 12hr old		15
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		30
white prepupae new		60
white prepupae 12hr		38
white prepupae 24hr		19
pupae 2d postWPP		14
pupae 3d postWPP		9
pupae 4d postWPP		11
adult male 01day		29
adult male 05day		24
adult male 30day		24
adult female 01day		21
adult female 05day		24
adult female 30day		22
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		29
embryo 02-04hr		10
embryo 04-06hr		16
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		11
embryo 12-14hr		9
embryo 14-16hr		8
embryo 16-18hr		9
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		17
larva L1		20
larva L2		14
larva L3 12hr old		15
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		30
white prepupae new		(60)
white prepupae 12hr		(38)
white prepupae 24hr		19
pupae 2d postWPP		14
pupae 3d postWPP		9
pupae 4d postWPP		11
adult male 01day		29
adult male 05day		24
adult male 30day		24
adult female 01day		21
adult female 05day		24
adult female 30day		22
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		29
embryo 02-04hr		10
embryo 04-06hr		16
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		11
embryo 12-14hr		9
embryo 14-16hr		8
embryo 16-18hr		9
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		17
larva L1		20
larva L2		14
larva L3 12hr old		15
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		30
white prepupae new		60
white prepupae 12hr		38
white prepupae 24hr		19
pupae 2d postWPP		14
pupae 3d postWPP		9
pupae 4d postWPP		11
adult male 01day		29
adult male 05day		24
adult male 30day		24
adult female 01day		21
adult female 05day		24
adult female 30day		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		29
embryo 02-04hr		10
embryo 04-06hr		16
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		11
embryo 12-14hr		9
embryo 14-16hr		8
embryo 16-18hr		9
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		17
larva L1		20
larva L2		14
larva L3 12hr old		15
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		30
white prepupae new		60
white prepupae 12hr		38
white prepupae 24hr		19
pupae 2d postWPP		14
pupae 3d postWPP		9
pupae 4d postWPP		11
adult male 01day		29
adult male 05day		24
adult male 30day		24
adult female 01day		21
adult female 05day		24
adult female 30day		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		37
Schneider line 2 Sg4		14
embryonic 1182-4H		19
embryonic GM2		20
embryonic Kc167		40
embryonic S1		18
embryonic S3		12
leg disc CME L1		12
wing disc CME-W2		14
wing disc ML-DmD8		14
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		18
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		24
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		15
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		18
tumorous blood cells mbn2		23
ovary fGS/OSS		27
ovary OSC		27
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(37)
Schneider line 2 Sg4		14
embryonic 1182-4H		19
embryonic GM2		20
embryonic Kc167		(40)
embryonic S1		18
embryonic S3		12
leg disc CME L1		12
wing disc CME-W2		14
wing disc ML-DmD8		14
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		18
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		24
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		15
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		18
tumorous blood cells mbn2		23
ovary fGS/OSS		(27)
ovary OSC		(27)
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		37
Schneider line 2 Sg4		14
embryonic 1182-4H		19
embryonic GM2		20
embryonic Kc167		40
embryonic S1		18
embryonic S3		12
leg disc CME L1		12
wing disc CME-W2		14
wing disc ML-DmD8		14
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		18
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		24
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		15
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		18
tumorous blood cells mbn2		23
ovary fGS/OSS		27
ovary OSC		27
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		37
Schneider line 2 Sg4		14
embryonic 1182-4H		19
embryonic GM2		20
embryonic Kc167		40
embryonic S1		18
embryonic S3		12
leg disc CME L1		12
wing disc CME-W2		14
wing disc ML-DmD8		14
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		18
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		24
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		15
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		18
tumorous blood cells mbn2		23
ovary fGS/OSS		27
ovary OSC		27
ovary OSS		11
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		37
Schneider line 2 Sg4		14
embryonic 1182-4H		19
embryonic GM2		20
embryonic Kc167		40
embryonic S1		18
embryonic S3		12
leg disc CME L1		12
wing disc CME-W2		14
wing disc ML-DmD8		14
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		18
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		24
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		15
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		18
tumorous blood cells mbn2		23
ovary fGS/OSS		27
ovary OSC		27
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(37)
Schneider line 2 Sg4		14
embryonic 1182-4H		19
embryonic GM2		20
embryonic Kc167		(40)
embryonic S1		18
embryonic S3		12
leg disc CME L1		12
wing disc CME-W2		14
wing disc ML-DmD8		14
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		18
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		24
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		15
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		18
tumorous blood cells mbn2		23
ovary fGS/OSS		27
ovary OSC		27
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		37
Schneider line 2 Sg4		14
embryonic 1182-4H		19
embryonic GM2		20
embryonic Kc167		40
embryonic S1		18
embryonic S3		12
leg disc CME L1		12
wing disc CME-W2		14
wing disc ML-DmD8		14
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		18
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		24
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		15
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		18
tumorous blood cells mbn2		23
ovary fGS/OSS		27
ovary OSC		27
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		37
Schneider line 2 Sg4		14
embryonic 1182-4H		19
embryonic GM2		20
embryonic Kc167		40
embryonic S1		18
embryonic S3		12
leg disc CME L1		12
wing disc CME-W2		14
wing disc ML-DmD8		14
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		18
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		24
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		15
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		18
tumorous blood cells mbn2		23
ovary fGS/OSS		27
ovary OSC		27
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		64
cold shock, 4-day adult		63
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		24
Cadmium 50 mM 48 hrs, 4-day adult		18
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		17
Copper 15 mM 48 hrs, 4-day adult		31
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		38
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		16
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		41
Caffeine 25 mg/ml 48 hrs, 4-day adult		66
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(64)
cold shock, 4-day adult		(63)
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		24
Cadmium 50 mM 48 hrs, 4-day adult		18
Cadmium 100 mM 48 hrs, 4-day adult		(27)
Copper 0.5 mM 12 hrs, larvae L3		17
Copper 15 mM 48 hrs, 4-day adult		(31)
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		(38)
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		16
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(41)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(66)
Paraquat 5 mM 48 hrs, 4-day adult		(27)
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		64
cold shock, 4-day adult		63
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		24
Cadmium 50 mM 48 hrs, 4-day adult		18
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		17
Copper 15 mM 48 hrs, 4-day adult		31
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		38
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		16
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		41
Caffeine 25 mg/ml 48 hrs, 4-day adult		66
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		64
cold shock, 4-day adult		63
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		24
Cadmium 50 mM 48 hrs, 4-day adult		18
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		17
Copper 15 mM 48 hrs, 4-day adult		31
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		38
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		16
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		41
Caffeine 25 mg/ml 48 hrs, 4-day adult		66
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		64
cold shock, 4-day adult		63
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		24
Cadmium 50 mM 48 hrs, 4-day adult		18
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		17
Copper 15 mM 48 hrs, 4-day adult		31
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		38
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		16
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		41
Caffeine 25 mg/ml 48 hrs, 4-day adult		66
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(64)
cold shock, 4-day adult		(63)
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		24
Cadmium 50 mM 48 hrs, 4-day adult		18
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		17
Copper 15 mM 48 hrs, 4-day adult		31
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		(38)
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		16
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(41)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(66)
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		64
cold shock, 4-day adult		63
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		24
Cadmium 50 mM 48 hrs, 4-day adult		18
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		17
Copper 15 mM 48 hrs, 4-day adult		31
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		38
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		16
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		41
Caffeine 25 mg/ml 48 hrs, 4-day adult		66
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		64
cold shock, 4-day adult		63
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		24
Cadmium 50 mM 48 hrs, 4-day adult		18
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		17
Copper 15 mM 48 hrs, 4-day adult		31
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		38
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		16
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		41
Caffeine 25 mg/ml 48 hrs, 4-day adult		66
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		22
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

SD05789

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0035436

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG12016-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

CG12016^GD12641

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of CG12016	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG12016	Mutagen	Stocks	Known lesion
CG12016^GD12641	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
CG12016^KK101721	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG12016^NIG.12016R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3L)Exel6093

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD12641}

P{KK101721}

P{NIG.12016R}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}EY02553

Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 2 terms )

Molecular Function

CV term

Evidence

References

ribosylnicotinamide kinase activity

inferred from electronic annotation with InterPro:IPR026681

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

nicotinamide nucleotide biosynthetic process

inferred from electronic annotation with InterPro:IPR026681

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

gene_with_dicistronic_mRNA

(FlyBase Genome Annotators, 2008)

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG12016 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

63907

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0035436

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

38411

Orthologs

OrthoDB Orthologs (31) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG60K8K2

Dipteran inclusive ortholog search

EOG6Q58ZB

Insect inclusive ortholog search

EOG6J6QN6

Arthropod inclusive ortholog search

EOG6FBG94

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG60K8K2)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11339

Drosophila persimilis

Dper\GL16138

Drosophila willistoni

Dwil\GK20507

Drosophila virilis

Dvir\GJ12857

Drosophila mojavensis

Dmoj\GI16604

Drosophila grimshawi

Dgri\GH15952

Orthologs in non-Drosophila Dipterans (EOG6Q58ZB)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL005106

Anopheles gambiae

Malaria mosquito

Agam\AGAP006740

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ000458

Orthologs in non-Dipteran Insects (EOG6J6QN6)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB53410

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000227

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH18439

Atta cephalotes

Leafcutter ant

Acep\ACEP10544

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO19325

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL14002

Linepithema humile

Argentine ant

Lhum\LH22240

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB22800

Solenopsis invicta

Red fire ant

Sinv\SINV20185

Solenopsis invicta

Red fire ant

Sinv\SINV19641

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004655

Bombyx mori

Silkmoth

Bmor\BGIBMGA013811

Pediculus humanus

Human body louse

Phum\PHUM105920

Tribolium castaneum

Red flour beetle

Tcas\TC011546

Orthologs in non-Insect Arthropods (EOG6FBG94)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\PHYC

Ixodes scapularis

Deer tick

Isca\ISCW019415

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11339

Drosophila persimilis

Dper\GL16138

Drosophila willistoni

Dwil\GK20507

Drosophila virilis

Dvir\GJ12857

Drosophila mojavensis

Dmoj\GI16604

Drosophila grimshawi

Dgri\GH15952

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v103613

P{KK101721}VIE-260B

v22871

w¹¹¹⁸; P{GD12641}v22871

v22872

w¹¹¹⁸; P{GD12641}v22872/TM3

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 56 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

SD05789

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0035436

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

38411

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

One or more of the processed transcripts for these genes contain two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG12016 (FBgn0035436), CG42456 (FBgn0259933).

(FlyBase Genome Annotators, 2008)

Other Comments

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; increased expression in per⁰¹, tim⁰¹ and Clk^Jrk background.

(Claridge-Chang et al., 2001)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•