FlyBase Gene Report: Dmel\Fdx2

General Information

Symbol

Dmel\Fdx2

Species

D. melanogaster

Name

Ferredoxin 2

Annotation Symbol

CG1319

Feature Type

protein_coding_gene

FlyBase ID

FBgn0035529

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Ferredoxin 2 (Fdx2) encodes a protein involved in ecdysteroid biosynthesis regulation. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

64B1-64B1

Sequence location

3L:4,292,482..4,293,432 [+]

Recombination map

3-12

RefSeq locus

NT_037436 REGION:4292482..4293432

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables 2 iron, 2 sulfur cluster binding

inferred from electronic annotation with InterPro:IPR001055, InterPro:IPR018298

(InterPro Project Members, 2004-)

enables electron transfer activity

inferred from sequence or structural similarity with UniProtKB:P10109

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN002511924

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in positive regulation of ecdysteroid biosynthetic process

inferred from mutant phenotype

(Palandri et al., 2015)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in electron transport chain

inferred from biological aspect of ancestor with PANTHER:PTN002511924

(GO Reference Genome Project, 2011-)

involved_in iron-sulfur cluster assembly

non-traceable author statement

(Marelja et al., 2018)

involved_in P450-containing electron transport chain

inferred from electronic annotation with InterPro:IPR001055, InterPro:IPR018298

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in mitochondrial matrix

non-traceable author statement

(Marelja et al., 2018)

located_in mitochondrial matrix

inferred from sequence or structural similarity with UniProtKB:P24483

(Gene Ontology Curators, 2002-)

is_active_in mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000603547

(GO Reference Genome Project, 2011-)

located_in mitochondrion

inferred from sequence or structural similarity with UniProtKB:P10109

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the adrenodoxin/putidaredoxin family. (Q8SZA8)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Ferredoxin 2 (Fdx2) encodes a protein involved in ecdysteroid biosynthesis regulation. [Date last reviewed: 2019-09-19]

Protein Function (UniProtKB)

Required for ecdysteroidogenesis in the prothoracic gland which is necessary for larval to pupal transition.

(UniProt, Q8SZA8)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Fdx2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8SZA8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Fdx2-RB

FBtr0300775

NM_139632

863

152

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Fdx2-PB

FBpp0289999

16.4

152

4.95

Q8SZA8

NP_647889

AAF47883

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Fdx2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG1319_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.48

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 9 -- 10

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic anal pad

(Fisher et al., 2012)

embryonic epipharynx

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic hypopharynx

(Fisher et al., 2012)

embryonic large intestine

(Fisher et al., 2012)

embryonic small intestine

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

gastric caecum

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Fdx2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Fdx2

View exonic expression by tissue for Dmel\Fdx2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035529

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38530/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035529

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035529

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035529

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1319

Flygut - An atlas of the Drosophila adult midgut

FBgn0035529

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Fdx2

Stages(s) 1-3
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Fdx2

Transgenic constructs containing regulatory region of Fdx2

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel8098

(Ryder, 2004.4.26)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during third instar larval stage, with Scer\GAL4^phtm.PU

Fdx2^KK102957

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Fdx2^GD7956

viable, with Scer\GAL4^Mef2.PR

Fdx2^GD7956

viable, with Scer\GAL4^pnr-MD237

Fdx2^GD7956

viable, with Scer\GAL4^tin.CΔ4

Fdx2^GD7956

Other Phenotypes

Allele

abnormal developmental rate, with Scer\GAL4^da.G32

Fdx2^KK102957

abnormal developmental rate, with Scer\GAL4^phtm.PU

Fdx2^KK102957

decreased body size | larval stage, with Scer\GAL4^da.G32

Fdx2^KK102957

decreased body size | larval stage, with Scer\GAL4^phtm.PU

Fdx2^KK102957

some die during larval stage, with Scer\GAL4^da.G32

Fdx2^KK102957

visible, with Scer\GAL4^pnr-MD237

Fdx2^GD7956

Phenotype manifest in

Allele

tracheal section | larval stage, with Scer\GAL4^da.G32

Fdx2^KK102957

tracheal section | larval stage, with Scer\GAL4^phtm.PU

Fdx2^KK102957

trichogen cell, with Scer\GAL4^pnr-MD237

Fdx2^GD7956

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\FDX1

12 of 14

Yes

Hsap\FDX2

5 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Fdx1

12 of 14

Yes

Rnor\Fdx2

5 of 14

Mus musculus (laboratory mouse) (2)

Mmus\Fdx1

12 of 14

Yes

Mmus\Fdx2

4 of 14

Xenopus tropicalis (Western clawed frog) (4)

Xtro\fdx1

10 of 13

Yes

Xtro\XB5934217

6 of 13

Yes

Xtro\fdx2

1 of 13

Xtro\lrch2

1 of 13

Danio rerio (Zebrafish) (3)

Drer\fdx1

13 of 14

Yes

Drer\fdx1b

11 of 14

Yes

Drer\fdx2

4 of 14

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\Y73F8A.27

5 of 14

Yes

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP003212

12 of 12

Yes

Agam\AgaP_AGAP006799

4 of 12

Arabidopsis thaliana (thale-cress) (11)

Atha\MFDX1

6 of 13

Yes

Atha\MFDX2

6 of 13

Yes

Atha\AT3G07480

4 of 13

Yes

Atha\AT4G32590

2 of 13

Yes

Atha\PnsB3

2 of 13

Yes

Atha\FD1

1 of 13

Yes

Atha\FD3

1 of 13

Yes

Atha\FD4

1 of 13

Yes

Atha\FdC1

1 of 13

Yes

Atha\FdC2

1 of 13

Yes

Atha\FED A

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\YAH1

5 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\etp1

3 of 12

Yes

Escherichia coli (enterobacterium) (3)

Ecol\fdx

5 of 11

Yes

Ecol\paaE

1 of 11

Ecol\ycbX

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Fdx2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

Fdx1

8 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

12 of 14

5 of 14

MITOCHONDRIAL MYOPATHY, EPISODIC, WITH OR WITHOUT OPTIC ATROPHY AND REVERSIBLE LEUKOENCEPHALOPATHY; MEOAL

mitochondrial myopathy

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64007

DroID - A comprehensive database of gene and protein interactions.

FBgn0035529

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-12

Cytogenetic map

64B1-64B1

Sequence location

3L:4,292,482..4,293,432 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

64B1-64B1

Limits computationally determined from genome sequence between P{PZ}l(3)rG166^rG166 and P{PZ}sinu⁰⁶⁵²⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-12

(FlyBase, 2016)

3-9.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

62373

y¹ v¹; P{TRiP.HMJ23768}attP40/CyO

67827

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05782}attP40

NIG-Fly

HMJ23768

y¹ v¹; P{TRiP.HMJ23768}attP40/CyO

VDRC

v106473

P{KK102957}VIE-260B

v39792

w¹¹¹⁸; P{GD7956}v39792

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (51)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0035529

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0035529

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG1319 BcDNA:RH09920

(FlyBase, 1996-)

Source for merge of: CG1319 BcDNA:RE08888

(FlyBase, 1996-)

Additional comments

Source for merge of CG1319 BcDNA:RH09920 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Source for merge of CG1319 BcDNA:RE08888 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Fdx2 CG1319

(Palandri et al., 2015)

Nomenclature comments

FlyBase curator comment: Used 'Fdx2' rather than 'Fdxh2' as the symbol as its unclear what the 'h' indicates, and the mammalian orthologs use 'FDX'.

(Palandri et al., 2015)

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

BcDNA:RE08888

BcDNA:RH09920

CG1319

(Palandri et al., 2015, Luck et al., 2014, Cammarato et al., 2011, Keleman et al., 2009.8.5, Perkins et al., 2009)

Fdx2

(Vásquez-Procopio et al., 2020, Ou et al., 2016)

Fdxh2

(Palandri et al., 2015)

fdx2

(Ma et al., 2024)

Name Synonyms

Ferredoxin 2

ferredoxin

(Palandri et al., 2015)

Secondary FlyBase IDs

FBgn0047176
FBgn0062960

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38530

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8SZA8

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8SZA8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8SZA8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035529

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38530/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035529

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035529

FlyMine - An integrated database for Drosophila genomics

FBgn0035529

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1319

MARRVEL_MODEL - MARRVEL (model organism gene)

38530

Linkouts

BioGRID - A database of protein and genetic interactions.

64007

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0035529

DroID - A comprehensive database of gene and protein interactions.

FBgn0035529

DRSC - Results frm RNAi screens

FBgn0035529

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG1319_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035529

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0035529

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1319

Flygut - An atlas of the Drosophila adult midgut

FBgn0035529

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0035529

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC016223

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

References (31)

The following external sites may use different assemblies or annotations than FlyBase.

0.48

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