FB2025_05 , released December 11, 2025
Gene: Dmel\Fitm
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General Information
Symbol
Dmel\Fitm
Species
D. melanogaster
Name
Fat storage inducing transmembrane protein
Annotation Symbol
CG10671
Feature Type
protein_coding_gene
FlyBase ID
FBgn0035586
Gene Model Status
Current
Stock Availability
6 publicly available
Gene Summary
Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate (By similarity). Preferentially hydrolyzes unsaturated long-chain acyl-CoA substrates in the endoplasmic reticulum (ER) lumen (By similarity). This catalytic activity is required for maintaining ER structure and for lipid droplets (LDs) biogenesis, which are lipid storage organelles involved in maintaining lipid and energy homeostasis (By similarity) (PubMed:28067622). May directly bind to diacylglycerol (DAGs) and triacylglycerol, which is also important for LD biogenesis (By similarity). May support directional budding of nacent LDs from the ER into the cytosol by reducing DAG levels at sites of LD formation (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization (By similarity) (PubMed:28067622). Required for correct morphology of nociceptive multi-dendritic sensory neurons (PubMed:28067622). Required for normal mechanical amplification in hearing (PubMed:28067622). (UniProt, Q9VRJ2)
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
64C7-64C7
Sequence location
Recombination map
3-13
RefSeq locus
NT_037436 REGION:5144082..5146063
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables coenzyme A diphosphatase activity
inferred from sequence or structural similarity with UniProtKB:Q8N6M3
(Gene Ontology Curators, 2002-)
inferred from electronic annotation with InterPro:IPR019388
(InterPro Project Members, 2004-)
Biological Process (10 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in adult locomotory behavior
inferred from mutant phenotype
(Zazo Seco et al., 2017)
involved_in lipid droplet organization
inferred from mutant phenotype
(Zazo Seco et al., 2017)
involved_in regulation of dendrite development
inferred from mutant phenotype
(Zazo Seco et al., 2017)
involved_in sensory perception of sound
inferred from mutant phenotype
(Zazo Seco et al., 2017)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
involved_in fatty-acyl-CoA catabolic process
inferred from sequence or structural similarity with UniProtKB:Q8N6M3
(Gene Ontology Curators, 2002-)
involved_in lipid droplet formation
inferred from sequence or structural similarity with UniProtKB:Q8N6M3
(Gene Ontology Curators, 2002-)
involved_in lipid droplet organization
inferred from biological aspect of ancestor with PANTHER:PTN000565881
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR046401
(InterPro Project Members, 2004-)
involved_in lipid homeostasis
inferred from sequence or structural similarity with UniProtKB:Q8N6M3
(Gene Ontology Curators, 2002-)
involved_in lipid storage
inferred from biological aspect of ancestor with PANTHER:PTN000565881
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR019388, InterPro:IPR046401
(InterPro Project Members, 2004-)
involved_in phospholipid biosynthetic process
inferred from biological aspect of ancestor with PANTHER:PTN000565881
(GO Reference Genome Project, 2011-)
involved_in triglyceride storage
inferred from sequence or structural similarity with MGI:MGI:2444508
(Kadereit et al., 2008)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in endoplasmic reticulum membrane
inferred from biological aspect of ancestor with PANTHER:PTN000565881
(GO Reference Genome Project, 2011-)
located_in endoplasmic reticulum membrane
inferred from sequence or structural similarity with MGI:MGI:2444508
(Kadereit et al., 2008)
inferred from electronic annotation with InterPro:IPR019388
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the FIT family. FIT2 subfamily. (Q9VRJ2)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
COENZYME A DIPHOSPHATASES -
Coenzyme A diphosphatases catalyze the hydrolysis of coenzyme A to generate adenosine 3',5'-bisphosphate and (R)-4'-phosphopantetheine.
Protein Function (UniProtKB)
Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate (By similarity). Preferentially hydrolyzes unsaturated long-chain acyl-CoA substrates in the endoplasmic reticulum (ER) lumen (By similarity). This catalytic activity is required for maintaining ER structure and for lipid droplets (LDs) biogenesis, which are lipid storage organelles involved in maintaining lipid and energy homeostasis (By similarity) (PubMed:28067622). May directly bind to diacylglycerol (DAGs) and triacylglycerol, which is also important for LD biogenesis (By similarity). May support directional budding of nacent LDs from the ER into the cytosol by reducing DAG levels at sites of LD formation (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization (By similarity) (PubMed:28067622). Required for correct morphology of nociceptive multi-dendritic sensory neurons (PubMed:28067622). Required for normal mechanical amplification in hearing (PubMed:28067622).
(UniProt, Q9VRJ2)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Fitm for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VRJ2)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Fitm-RA
FBtr0077178
NM_139687
1849
423
Fitm-RB
FBtr0077177
NM_168110
1923
480
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Fitm-PA
FBpp0076881
47.9
423
10.63
Q9VRJ2
NP_647944
AAF50803
Fitm-PB
FBpp0076880
54.6
480
10.54
NP_729053
AAN12239
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Fitm using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.00

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Fitm in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Fitm
Transgenic constructs containing regulatory region of Fitm
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3L)Exel6103
(Ryder, 2004.4.26)
Inferred from location ( 8 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\FITM2
13 of 14
Yes
Yes
1  
Hsap\FITM1
6 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Fitm2
13 of 14
Yes
Yes
Rnor\Fitm1
6 of 14
No
Yes
Mus musculus (laboratory mouse) (2)
Mmus\Fitm2
11 of 14
Yes
Yes
Mmus\Fitm1
8 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
Xtro\fitm2
10 of 13
Yes
Yes
Xtro\fitm1
2 of 13
No
No
Xtro\gdap2
1 of 13
No
No
Danio rerio (Zebrafish) (3)
Drer\fitm2
13 of 14
Yes
Yes
Drer\fitm1l
4 of 14
No
Yes
Drer\LOC792443
4 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\fitm-2
13 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP006131
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\SCS3
6 of 13
Yes
Yes
Scer\YFT2
5 of 13
No
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPBC543.08
9 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Fitm. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Potential Models Based on Orthology ( 1 )
Human Ortholog
Disease
Evidence
References
FITM2; fat storage inducing transmembrane protein 2
model of  Siddiqi syndrome
IEA
(FlyBase, 2019-)
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
FITM2; fat storage inducing transmembrane protein 2
13 of 14
612029
 
FITM1; fat storage inducing transmembrane protein 1
6 of 14
612028
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Fitm - stumps
      two hybrid
      (Battersby et al., 2003)
      Summary of Genetic Interactions
      Interaction Browsers
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
      External Data
      Linkouts
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map
      3-13
      Cytogenetic map
      64C7-64C7
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      64C7-64C7
      Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      3-13

      (FlyBase, 2016)

      3-12.2

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (6)
      Genomic Clones (8)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (125)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments
            Relationship to Other Genes
            Source for database merge of
            Additional comments
            Nomenclature History
            Source for database identify of

            Source for identity of: Fitm CG10671

            (Zazo Seco et al., 2017)
            Nomenclature comments
            Etymology

            Named 'Fitm' after the human orthologs.

            (Zazo Seco et al., 2017)
            Synonyms and Secondary IDs (5)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 36 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
            References (35)