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Gene Dmel\mad2

General Information
Symbol	Dmel\mad2	Species	D. melanogaster
Name	mad2	Annotation symbol	CG17498
Feature type	protein_coding_gene	FlyBase ID	FBgn0035640
Gene Model Status	Current	Stock availability	9 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	64E11-64E12	Sequence location	3L:5,805,235..5,806,193 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene mad2 is referred to in FlyBase by the symbol Dmel\mad2 (CG17498, FBgn0035640). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: mitotic spindle assembly checkpoint; regulation of mitotic prometaphase; mitotic cell cycle checkpoint; regulation of spindle assembly involved in mitosis; cell cycle; regulation of spindle checkpoint. 14 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA-binding HORMA; Mitotic spindle checkpoint protein Mad2. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1640938_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of mad2. Gene sequence location is 3L:5805235..5806193.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms regulation of spindle assembly involved in mitosis
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 64E11-64E12   Limits computationally determined from genome sequence between P{PZ}Bre101640 and P{PZ}vn10567
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mad2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.45 Low-frequency RNA-seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mad2-RA FBtr0077113  NM_139734   818   207
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mad2-PA   FBpp0076819   23.4   207   5.72     NP_647991   AAF50740
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • DNA-binding HORMA (IPR003511) Mitotic spindle checkpoint protein Mad2 (IPR027097)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF50740   AY070619 AAL48090   BI578221   BI579041   BP556104   CO267385   CO305650   CO305949   CO311258   CZ471089   CZ471256   EC063685   EC067713   EC087665   EC088831   EC203494   EC210515   EC215587   EC225288   EC249748
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VRQ2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium head mesoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Buffin et al., 2005, Katsani et al., 2008) kinetochore inferred from direct assay (Buffin et al., 2005, Griffis et al., 2007, Katsani et al., 2008) kinetochore microtubule inferred from direct assay (Buffin et al., 2005) microtubule associated complex inferred from direct assay (Hughes et al., 2008) colocalizes_with mitotic spindle inferred from direct assay (Katsani et al., 2008) nuclear envelope inferred from direct assay (Buffin et al., 2005) colocalizes_with nuclear pore inferred from direct assay (Katsani et al., 2008) nucleoplasm inferred from direct assay (Katsani et al., 2008) spindle inferred from direct assay (Griffis et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    391.9 Larval Midgut    24.5 Larval Hindgut    30.9 Larval Malpighian Tubules    18.7 Larval Fat Body    52.5 Larval Salivary Gland    16.9 Larval Trachea    100.05 Larval Carcass    43.45 Adult Head    13.5 Adult Eye    10.875 Adult Brain    8.8 Adult Thoracic-Abdominal Ganglion    24.6 Adult Crop    18.5 Adult Midgut    18.1 Adult Hindgut    15.2 Adult Malpighian Tubules    13.7 Adult Fat Body    11.2 Adult Salivary Gland    10.8 Adult Heart    14.65 Adult VirginFemale Spermatheca    12.9 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    672.2 Adult Testis    251.2 Adult Male Accessory Gland    11.7 Adult Carcass    17.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    391.9 Larval Midgut    24.5 Larval Hindgut    30.9 Larval Malpighian Tubules    18.7 Larval Fat Body    52.5 Larval Salivary Gland    16.9 Larval Trachea    100.05 Larval Carcass    43.45 Adult Head    13.5 Adult Eye    10.875 Adult Brain    8.8 Adult Thoracic-Abdominal Ganglion    24.6 Adult Crop    18.5 Adult Midgut    18.1 Adult Hindgut    15.2 Adult Malpighian Tubules    13.7 Adult Fat Body    11.2 Adult Salivary Gland    10.8 Adult Heart    14.65 Adult VirginFemale Spermatheca    12.9 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary  (672.2) Adult Testis    251.2 Adult Male Accessory Gland    11.7 Adult Carcass    17.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    391.9 Larval Midgut    24.5 Larval Hindgut    30.9 Larval Malpighian Tubules    18.7 Larval Fat Body    52.5 Larval Salivary Gland    16.9 Larval Trachea    100.05 Larval Carcass    43.45 Adult Head    13.5 Adult Eye    10.875 Adult Brain    8.8 Adult Thoracic-Abdominal Ganglion    24.6 Adult Crop    18.5 Adult Midgut    18.1 Adult Hindgut    15.2 Adult Malpighian Tubules    13.7 Adult Fat Body    11.2 Adult Salivary Gland    10.8 Adult Heart    14.65 Adult VirginFemale Spermatheca    12.9 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    672.2 Adult Testis    251.2 Adult Male Accessory Gland    11.7 Adult Carcass    17.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    391.9 Larval Midgut    24.5 Larval Hindgut    30.9 Larval Malpighian Tubules    18.7 Larval Fat Body    52.5 Larval Salivary Gland    16.9 Larval Trachea    100.05 Larval Carcass    43.45 Adult Head    13.5 Adult Eye    10.875 Adult Brain    8.8 Adult Thoracic-Abdominal Ganglion    24.6 Adult Crop    18.5 Adult Midgut    18.1 Adult Hindgut    15.2 Adult Malpighian Tubules    13.7 Adult Fat Body    11.2 Adult Salivary Gland    10.8 Adult Heart    14.65 Adult VirginFemale Spermatheca    12.9 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    672.2 Adult Testis    251.2 Adult Male Accessory Gland    11.7 Adult Carcass    17.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    391.9 Larval Midgut    24.5 Larval Hindgut    30.9 Larval Malpighian Tubules    18.7 Larval Fat Body    52.5 Larval Salivary Gland    16.9 Larval Trachea    100.05 Larval Carcass    43.45 Adult Head    13.5 Adult Eye    10.875 Adult Brain    8.8 Adult Thoracic-Abdominal Ganglion    24.6 Adult Crop    18.5 Adult Midgut    18.1 Adult Hindgut    15.2 Adult Malpighian Tubules    13.7 Adult Fat Body    11.2 Adult Salivary Gland    10.8 Adult Heart    14.65 Adult VirginFemale Spermatheca    12.9 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    672.2 Adult Testis    251.2 Adult Male Accessory Gland    11.7 Adult Carcass    17.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    391.9 Larval Midgut    24.5 Larval Hindgut    30.9 Larval Malpighian Tubules    18.7 Larval Fat Body    52.5 Larval Salivary Gland    16.9 Larval Trachea    100.05 Larval Carcass    43.45 Adult Head    13.5 Adult Eye    10.875 Adult Brain    8.8 Adult Thoracic-Abdominal Ganglion    24.6 Adult Crop    18.5 Adult Midgut    18.1 Adult Hindgut    15.2 Adult Malpighian Tubules    13.7 Adult Fat Body    11.2 Adult Salivary Gland    10.8 Adult Heart    14.65 Adult VirginFemale Spermatheca    12.9 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary  672.2 Adult Testis    251.2 Adult Male Accessory Gland    11.7 Adult Carcass    17.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    391.9 Larval Midgut    24.5 Larval Hindgut    30.9 Larval Malpighian Tubules    18.7 Larval Fat Body    52.5 Larval Salivary Gland    16.9 Larval Trachea    100.05 Larval Carcass    43.45 Adult Head    13.5 Adult Eye    10.875 Adult Brain    8.8 Adult Thoracic-Abdominal Ganglion    24.6 Adult Crop    18.5 Adult Midgut    18.1 Adult Hindgut    15.2 Adult Malpighian Tubules    13.7 Adult Fat Body    11.2 Adult Salivary Gland    10.8 Adult Heart    14.65 Adult VirginFemale Spermatheca    12.9 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    672.2 Adult Testis    251.2 Adult Male Accessory Gland    11.7 Adult Carcass    17.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    391.9 Larval Midgut    24.5 Larval Hindgut    30.9 Larval Malpighian Tubules    18.7 Larval Fat Body    52.5 Larval Salivary Gland    16.9 Larval Trachea    100.05 Larval Carcass    43.45 Adult Head    13.5 Adult Eye    10.875 Adult Brain    8.8 Adult Thoracic-Abdominal Ganglion    24.6 Adult Crop    18.5 Adult Midgut    18.1 Adult Hindgut    15.2 Adult Malpighian Tubules    13.7 Adult Fat Body    11.2 Adult Salivary Gland    10.8 Adult Heart    14.65 Adult VirginFemale Spermatheca    12.9 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    672.2 Adult Testis    251.2 Adult Male Accessory Gland    11.7 Adult Carcass    17.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    no informative data   NA  Brain    8.8   391.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    24.6   NA  Crop    no informative data   24.5  Midgut    no informative data   30.9  Hindgut    no informative data   18.7  Malpighian Tubules    no informative data   52.5  Fat Body    no informative data   16.9  Salivary Gland    no informative data   NA  Heart    no informative data   100.05  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    672.2   NA  Testis    251.2   NA  Male Accessory Gland    no informative data   43.45  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3    33 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    7 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    12 fat body, pupae P8    24 carcass, larvae L3 wandering    17 carcass, 1-day adult    2 carcass, 4-day adult    8 carcass, 20-day adult    5 ovary, virgin 4-day female    125 ovary, mated 4-day female    131 testis, mated 4-day male    36 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (58) central nervous system, larvae L3  (33) central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    7 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    12 fat body, pupae P8    24 carcass, larvae L3 wandering    17 carcass, 1-day adult    2 carcass, 4-day adult    8 carcass, 20-day adult    5 ovary, virgin 4-day female  (125) ovary, mated 4-day female  (131) testis, mated 4-day male  (36) accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3    33 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    7 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    12 fat body, pupae P8    24 carcass, larvae L3 wandering    17 carcass, 1-day adult    2 carcass, 4-day adult    8 carcass, 20-day adult    5 ovary, virgin 4-day female  (125) ovary, mated 4-day female  (131) testis, mated 4-day male    36 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3    33 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    7 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    12 fat body, pupae P8    24 carcass, larvae L3 wandering    17 carcass, 1-day adult    2 carcass, 4-day adult    8 carcass, 20-day adult    5 ovary, virgin 4-day female    125 ovary, mated 4-day female    131 testis, mated 4-day male    36 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3    33 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    7 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    12 fat body, pupae P8    24 carcass, larvae L3 wandering    17 carcass, 1-day adult    2 carcass, 4-day adult    8 carcass, 20-day adult    5 ovary, virgin 4-day female    125 ovary, mated 4-day female    131 testis, mated 4-day male    36 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (58) central nervous system, larvae L3  (33) central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    7 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    12 fat body, pupae P8    24 carcass, larvae L3 wandering    17 carcass, 1-day adult    2 carcass, 4-day adult    8 carcass, 20-day adult    5 ovary, virgin 4-day female  (125) ovary, mated 4-day female  (131) testis, mated 4-day male  (36) accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3    33 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    7 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    12 fat body, pupae P8    24 carcass, larvae L3 wandering    17 carcass, 1-day adult    2 carcass, 4-day adult    8 carcass, 20-day adult    5 ovary, virgin 4-day female  125 ovary, mated 4-day female  131 testis, mated 4-day male    36 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3    33 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    7 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    12 fat body, pupae P8    24 carcass, larvae L3 wandering    17 carcass, 1-day adult    2 carcass, 4-day adult    8 carcass, 20-day adult    5 ovary, virgin 4-day female    125 ovary, mated 4-day female    131 testis, mated 4-day male    36 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    152 embryo 02-04hr    144 embryo 04-06hr    136 embryo 06-08hr    69 embryo 08-10hr    63 embryo 10-12hr    32 embryo 12-14hr    30 embryo 14-16hr    23 embryo 16-18hr    17 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    17 larva L1    2 larva L2    3 larva L3 12hr old    4 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    37 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    23 adult male 30day    22 adult female 01day    28 adult female 05day    75 adult female 30day    75 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (152) embryo 02-04hr  (144) embryo 04-06hr  (136) embryo 06-08hr  (69) embryo 08-10hr  (63) embryo 10-12hr  (32) embryo 12-14hr  (30) embryo 14-16hr    23 embryo 16-18hr    17 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    17 larva L1    2 larva L2    3 larva L3 12hr old    4 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr  (37) white prepupae 24hr  (29) pupae 2d postWPP    18 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    23 adult male 30day    22 adult female 01day  (28) adult female 05day  (75) adult female 30day  (75) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (152) embryo 02-04hr  (144) embryo 04-06hr  (136) embryo 06-08hr    69 embryo 08-10hr    63 embryo 10-12hr    32 embryo 12-14hr    30 embryo 14-16hr    23 embryo 16-18hr    17 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    17 larva L1    2 larva L2    3 larva L3 12hr old    4 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    37 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    23 adult male 30day    22 adult female 01day    28 adult female 05day    75 adult female 30day    75 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    152 embryo 02-04hr    144 embryo 04-06hr    136 embryo 06-08hr    69 embryo 08-10hr    63 embryo 10-12hr    32 embryo 12-14hr    30 embryo 14-16hr    23 embryo 16-18hr    17 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    17 larva L1    2 larva L2    3 larva L3 12hr old    4 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    37 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    23 adult male 30day    22 adult female 01day    28 adult female 05day    75 adult female 30day    75 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    152 embryo 02-04hr    144 embryo 04-06hr    136 embryo 06-08hr    69 embryo 08-10hr    63 embryo 10-12hr    32 embryo 12-14hr    30 embryo 14-16hr    23 embryo 16-18hr    17 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    17 larva L1    2 larva L2    3 larva L3 12hr old    4 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    37 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    23 adult male 30day    22 adult female 01day    28 adult female 05day    75 adult female 30day    75 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (152) embryo 02-04hr  (144) embryo 04-06hr  (136) embryo 06-08hr  (69) embryo 08-10hr  (63) embryo 10-12hr  32 embryo 12-14hr  30 embryo 14-16hr    23 embryo 16-18hr    17 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    17 larva L1    2 larva L2    3 larva L3 12hr old    4 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr  (37) white prepupae 24hr  29 pupae 2d postWPP    18 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    23 adult male 30day    22 adult female 01day  28 adult female 05day  (75) adult female 30day  (75) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (152) embryo 02-04hr  (144) embryo 04-06hr  136 embryo 06-08hr    69 embryo 08-10hr    63 embryo 10-12hr    32 embryo 12-14hr    30 embryo 14-16hr    23 embryo 16-18hr    17 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    17 larva L1    2 larva L2    3 larva L3 12hr old    4 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    37 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    23 adult male 30day    22 adult female 01day    28 adult female 05day    75 adult female 30day    75 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    152 embryo 02-04hr    144 embryo 04-06hr    136 embryo 06-08hr    69 embryo 08-10hr    63 embryo 10-12hr    32 embryo 12-14hr    30 embryo 14-16hr    23 embryo 16-18hr    17 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    17 larva L1    2 larva L2    3 larva L3 12hr old    4 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    37 white prepupae 24hr    29 pupae 2d postWPP    18 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    23 adult male 30day    22 adult female 01day    28 adult female 05day    75 adult female 30day    75 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    108 Schneider line 2 Sg4    28 embryonic 1182-4H    56 embryonic GM2    32 embryonic Kc167    32 embryonic S1    50 embryonic S3    43 leg disc CME L1    59 wing disc CME-W2    29 wing disc ML-DmD8    31 wing disc ML-DmD9    78 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    39 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    66 eye-antennal disc ML-DmD11    37 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    48 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    67 ovary fGS/OSS    81 ovary OSC    83 ovary OSS    54 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (108) Schneider line 2 Sg4  (28) embryonic 1182-4H  (56) embryonic GM2  (32) embryonic Kc167  (32) embryonic S1  (50) embryonic S3  (43) leg disc CME L1  (59) wing disc CME-W2  (29) wing disc ML-DmD8  (31) wing disc ML-DmD9  (78) wing disc ML-DmD16-c3  (52) wing disc ML-DmD21  (39) wing disc ML-DmD32  (33) haltere disc ML-DmD17-c3  (66) eye-antennal disc ML-DmD11  (37) antennal disc ML-DmD20-c5  (59) mixed discs ML-DmD4-c1  (38) CNS ML-DmBG1-c1  (48) CNS ML-DmBG2-c2  (29) tumorous blood cells mbn2  (67) ovary fGS/OSS  (81) ovary OSC  (83) ovary OSS  (54) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (108) Schneider line 2 Sg4    28 embryonic 1182-4H    56 embryonic GM2    32 embryonic Kc167    32 embryonic S1    50 embryonic S3    43 leg disc CME L1    59 wing disc CME-W2    29 wing disc ML-DmD8    31 wing disc ML-DmD9    78 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    39 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    66 eye-antennal disc ML-DmD11    37 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    48 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    67 ovary fGS/OSS    81 ovary OSC    83 ovary OSS    54 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    108 Schneider line 2 Sg4    28 embryonic 1182-4H    56 embryonic GM2    32 embryonic Kc167    32 embryonic S1    50 embryonic S3    43 leg disc CME L1    59 wing disc CME-W2    29 wing disc ML-DmD8    31 wing disc ML-DmD9    78 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    39 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    66 eye-antennal disc ML-DmD11    37 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    48 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    67 ovary fGS/OSS    81 ovary OSC    83 ovary OSS    54 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    108 Schneider line 2 Sg4    28 embryonic 1182-4H    56 embryonic GM2    32 embryonic Kc167    32 embryonic S1    50 embryonic S3    43 leg disc CME L1    59 wing disc CME-W2    29 wing disc ML-DmD8    31 wing disc ML-DmD9    78 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    39 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    66 eye-antennal disc ML-DmD11    37 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    48 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    67 ovary fGS/OSS    81 ovary OSC    83 ovary OSS    54 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (108) Schneider line 2 Sg4  28 embryonic 1182-4H  (56) embryonic GM2  32 embryonic Kc167  32 embryonic S1  (50) embryonic S3  (43) leg disc CME L1  (59) wing disc CME-W2  29 wing disc ML-DmD8  31 wing disc ML-DmD9  (78) wing disc ML-DmD16-c3  (52) wing disc ML-DmD21  (39) wing disc ML-DmD32  (33) haltere disc ML-DmD17-c3  (66) eye-antennal disc ML-DmD11  (37) antennal disc ML-DmD20-c5  (59) mixed discs ML-DmD4-c1  (38) CNS ML-DmBG1-c1  (48) CNS ML-DmBG2-c2  29 tumorous blood cells mbn2  (67) ovary fGS/OSS  (81) ovary OSC  (83) ovary OSS  (54) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  108 Schneider line 2 Sg4    28 embryonic 1182-4H    56 embryonic GM2    32 embryonic Kc167    32 embryonic S1    50 embryonic S3    43 leg disc CME L1    59 wing disc CME-W2    29 wing disc ML-DmD8    31 wing disc ML-DmD9    78 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    39 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    66 eye-antennal disc ML-DmD11    37 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    48 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    67 ovary fGS/OSS    81 ovary OSC    83 ovary OSS    54 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    108 Schneider line 2 Sg4    28 embryonic 1182-4H    56 embryonic GM2    32 embryonic Kc167    32 embryonic S1    50 embryonic S3    43 leg disc CME L1    59 wing disc CME-W2    29 wing disc ML-DmD8    31 wing disc ML-DmD9    78 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    39 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    66 eye-antennal disc ML-DmD11    37 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    48 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    67 ovary fGS/OSS    81 ovary OSC    83 ovary OSS    54 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    32 cold shock, 4-day adult    21 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    78 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    84 Caffeine 25 mg/ml 48 hrs, 4-day adult    49 Paraquat 5 mM 48 hrs, 4-day adult    68 Paraquat 10 mM 48 hrs, 4-day adult    42 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (32) cold shock, 4-day adult    21 heat shock, 4-day adult  (29) Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult  (39) Cadmium 100 mM 48 hrs, 4-day adult  (78) Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult  (63) Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3  (73) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (84) Caffeine 25 mg/ml 48 hrs, 4-day adult  (49) Paraquat 5 mM 48 hrs, 4-day adult  (68) Paraquat 10 mM 48 hrs, 4-day adult  (42) Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    32 cold shock, 4-day adult    21 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    78 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    84 Caffeine 25 mg/ml 48 hrs, 4-day adult    49 Paraquat 5 mM 48 hrs, 4-day adult    68 Paraquat 10 mM 48 hrs, 4-day adult    42 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    32 cold shock, 4-day adult    21 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    78 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    84 Caffeine 25 mg/ml 48 hrs, 4-day adult    49 Paraquat 5 mM 48 hrs, 4-day adult    68 Paraquat 10 mM 48 hrs, 4-day adult    42 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    32 cold shock, 4-day adult    21 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    78 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    84 Caffeine 25 mg/ml 48 hrs, 4-day adult    49 Paraquat 5 mM 48 hrs, 4-day adult    68 Paraquat 10 mM 48 hrs, 4-day adult    42 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  32 cold shock, 4-day adult    21 heat shock, 4-day adult  29 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult  (39) Cadmium 100 mM 48 hrs, 4-day adult  (78) Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult  (63) Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3  (73) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (84) Caffeine 25 mg/ml 48 hrs, 4-day adult  (49) Paraquat 5 mM 48 hrs, 4-day adult  (68) Paraquat 10 mM 48 hrs, 4-day adult  (42) Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    32 cold shock, 4-day adult    21 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    78 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    84 Caffeine 25 mg/ml 48 hrs, 4-day adult    49 Paraquat 5 mM 48 hrs, 4-day adult    68 Paraquat 10 mM 48 hrs, 4-day adult    42 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    32 cold shock, 4-day adult    21 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    78 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    84 Caffeine 25 mg/ml 48 hrs, 4-day adult    49 Paraquat 5 mM 48 hrs, 4-day adult    68 Paraquat 10 mM 48 hrs, 4-day adult    42 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts	BDGP expression data • RE72064 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035640 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17498-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for mad2 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal mad2GD9396 viable mad2G6595 mad2Δ viable, with Scer\GAL4pnr-MD237 mad2GD9396 Sterility Allele fertile mad2G6595 mad2Δ Other Phenotypes Allele cell cycle defective mad2G6595 mad2Δ developmental rate defective mad2G6595 mitotic cell cycle defective mad2G6595 mad2unspecified mad2Δ Phenotype manifest in Allele embryonic/larval brain | larval stage mad2GD9396
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of mad2 Class Mutagen Stocks Known lesion mad2c06928 piggyBac transposase 1 -- mad2c07120 piggyBac transposase 1 -- mad2EY21687 P-element activity 1 -- mad2G6595 0 Yes mad2unspecified 0 -- mad2Δ P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mad2 Class Mutagen Stocks Known lesion mad2GD16427 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes mad2GD9396 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes mad2KK108038 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mad2T:Avic\GFP-S65T in vitro construct - coding region fusion 1 Yes mad2NIG.17498R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes mad2Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes mad2T:Disc\RFP-mRFP in vitro construct - coding region fusion 0 -- mad2Ubi-p63E.T:Avic\GFP-S65T in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Exel6107 (Ryder, 2004.4.26)
Partially disrupted in	Df(3)07084 (Buffin et al., 2007)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9396} NA P{GD16427} NA P{KK108038} NA P{NIG.17498R} NA P{UAS-mad2.EGFP} NA characterization construct P{mad2-RFP} NA P{Ubi-GFP-Mad2} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}mad2c06928 NA PBac{PB}mad2c07120 NA insertion of mobile activating element P{EP}mad2G6595 NA P{EPgy2}mad2EY21687 NA
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 15 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cell cycle inferred from mutant phenotype (Kiger et al., 2003) mitotic cell cycle checkpoint inferred from mutant phenotype (Buffin et al., 2004) mitotic spindle assembly checkpoint inferred from direct assay (Logarinho et al., 2004) inferred from mutant phenotype (Orr et al., 2007) regulation of mitotic prometaphase inferred from mutant phenotype (Buffin et al., 2007) regulation of spindle assembly involved in mitosis inferred from genetic interaction with SAK (Basto et al., 2008) regulation of spindle checkpoint inferred from mutant phenotype (Buffin et al., 2007)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Buffin et al., 2005, Katsani et al., 2008) kinetochore inferred from direct assay (Buffin et al., 2005, Griffis et al., 2007, Katsani et al., 2008) kinetochore microtubule inferred from direct assay (Buffin et al., 2005) microtubule associated complex inferred from direct assay (Hughes et al., 2008) colocalizes_with mitotic spindle inferred from direct assay (Katsani et al., 2008) nuclear envelope inferred from direct assay (Buffin et al., 2005) colocalizes_with nuclear pore inferred from direct assay (Katsani et al., 2008) nucleoplasm inferred from direct assay (Katsani et al., 2008) spindle inferred from direct assay (Griffis et al., 2007)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mitotic spindle assembly checkpoint inferred from sequence or structural similarity with Saccharomyces MAD2 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with Saccharomyces MAD2 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
mad2 - Mad1 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) mad2 - ald experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mad2 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64116 DroID - A comprehensive database of gene and protein interactions. • FBgn0035640 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38656
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6V43V2 Dipteran inclusive ortholog search EOG6575GR Insect inclusive ortholog search EOG6SJ4B3 Arthropod inclusive ortholog search EOG695X80 Metazoa inclusive ortholog search EOG6CVV2J
Orthologs in Drosophila Species (EOG6V43V2)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\mad2     Drosophila simulans   Dsim\GD15958 Y   Drosophila sechellia   Dsec\GM13893 Y   Drosophila erecta   Dere\GG14107 Y   Drosophila yakuba   Dyak\GE20532 Y   Drosophila ananassae   Dana\GF10441 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA14530 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA26631     Drosophila persimilis   Dper\GL21408 Y   Drosophila persimilis   Dper\GL24493     Drosophila willistoni   Dwil\GK17532 Y   Drosophila virilis   Dvir\GJ13309 Y   Drosophila mojavensis   Dmoj\GI12039 Y   Drosophila grimshawi   Dgri\GH17035 Y
Orthologs in non-Drosophila Dipterans (EOG6575GR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008316   Anopheles gambiae Malaria mosquito  Agam\AGAP000751   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008480
Orthologs in non-Dipteran Insects (EOG6SJ4B3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Ame.16355   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG013159   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH22649   Atta cephalotes Leafcutter ant  Acep\ACEP12452   Camponotus floridanus Florida carpenter ant  Cflo\CFLO16524   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14126   Linepithema humile Argentine ant  Lhum\LH13699   Pogonomyrmex barbatus Red harvester ant  Pbar\PB16786   Solenopsis invicta Red fire ant  Sinv\SINV24983   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004152   Bombyx mori Silkmoth  Bmor\BGIBMGA012734   Pediculus humanus Human body louse  Phum\PHUM392720   Tribolium castaneum Red flour beetle  Tcas\mad2
Orthologs in non-Insect Arthropods (EOG695X80)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Tbc1d30   Ixodes scapularis Deer tick  Isca\ISCW001444
Orthologs in non-Arthropod Metazoa (EOG6CVV2J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\mdf-2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016042gn   Danio rerio Zebrafish  Drer\mad2l1   Xenopus tropicalis Western clawed frog  Xtro\mad2l1   Gallus gallus Domestic chicken  Ggal\MAD2L1   Mus musculus House mouse  Mmus\Mad2l1   Rattus norvegicus Norway rat  Rnor\Mad2l1   Homo sapiens Human  Hsap\MAD2L1
Human Orthologs (1)
Gene OMIM HGNC Hsap\MAD2L1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD15958 Drosophila sechellia Dsec\GM13893 Drosophila erecta Dere\GG14107 Drosophila yakuba Dyak\GE20532 Drosophila ananassae Dana\GF10441 Drosophila pseudoobscura pseudoobscura Dpse\GA14530 Drosophila persimilis Dper\GL21408 Drosophila willistoni Dwil\GK17532 Drosophila virilis Dvir\GJ13309 Drosophila mojavensis Dmoj\GI12039 Drosophila grimshawi Dgri\GH17035
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	35820 w*; Nup107E8; P{mad2.GFP}9a, P{mRFP-Nup107.K}7.1 22495 y1 w67c23; P{EPgy2}mad2EY21687/TM3, Sb1 Ser1
Harvard	- PBac{PB}mad2c06928 - PBac{PB}mad2c07120
VDRC	v106003 P{KK108038}VIE-260B v47918 w1118; P{GD16427}v47918 v47919 w1118; P{GD16427}v47919 v25262 w1118; P{GD9396}v25262/CyO v25264 w1118; P{GD9396}v25264/CyO
Genomic Clones ( 2 )
	BACR11A21 BACR29P05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 16 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE72064
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE73013
Other clones	37517 72576 101450 102312 146242 147048 159850 166179 273781 EK150010 EK223932 EK249159 EK249853 FGM301D09
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0035640 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38656
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: mad2 CG17498 (Buffin et al., 2004)
Source for database merge of
Additional comments
Other Comments
	dsRNA has been synthesised against this gene and transfected into S2 cells. (Griffis et al., 2007) dsRNA against this gene has been used to treat SL2 cells to study the effect of depletion of mad2 on the spindle checkpoint. (Muller et al., 2006) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cell morphology is aberrant, indicative of a failure in cell cycle/mitosis progression. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_139734 RefSeq (Proteins) • NP_647991 DDBJ / EMBL / GenBank • AAF50740 AAL48090 AY070619 BI578221 CZ471089 CZ471256 Entrez Gene - A searchable database of RefSeq genes. • 38656 UniProtKB/TrEMBL • Q9VRQ2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • DNA-binding HORMA (IPR003511) Mitotic spindle checkpoint protein Mad2 (IPR027097) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE72064
Linkouts
	BioGRID - A database of protein and genetic interactions • 64116 DroID - A comprehensive database of gene and protein interactions. • FBgn0035640 DRSC - Results from RNAi screens. • FBgn0035640 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035640 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17498-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0035640 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38656 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/mad2.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38656 modMine - Data generated by the modENCODE project. • FBgn0035640
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	CG17498 (Buffin, 2005, Buffin et al., 2005, Kiger et al., 2003, Egger et al., 2002, Dimova et al., 2003, Asha et al., 2003, Anholt and Mackay, 2001, Buffin et al., 2007, Keleman et al., 2009.8.5, Hughes et al., 2008, Loop et al., 2004, Gesellchen et al., 2005) mad2 (Muller et al., 2006, Karess, 2005, Hueber et al., 2007, Georlette et al., 2007, Basto et al., 2008, Buffin et al., 2007, McCleland et al., 2009, Rahmani et al., 2009, Schittenhelm et al., 2009, Royou et al., 2010, Li et al., 2010, Ebrahimi et al., 2010, Neumüller et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Althoff et al., 2012) Mad2 (Logarinho et al., 2004, Li et al., 2007, Griffis et al., 2007, Orr and Sunkel, 2011, Hickson and O'Farrell, 2008, Orr et al., 2007, Wen et al., 2008, Kwon et al., 2008, Pandey et al., 2007, Williams et al., 2007, Katsani et al., 2008, Lince-Faria et al., 2009, Mische et al., 2008, Andrejka et al., 2011, Ding et al., 2009, Matos et al., 2009, Oliveira et al., 2010, Przewloka et al., 2011, Korenjak et al., 2012, Batiha and Swan, 2012, Delgehyr et al., 2012, Emre et al., 2011) MAD2 (Garcia et al., 2007)
Name Synonym	mad2
Secondary FlyBase IDs
References ( 73 )
Generate a list of	All references relating to this gene ( 73 )
List References by type	Research paper  ( 46 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 4 ) FlyBase analysis  ( 1 ) Conference report  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 11 )
	Althoff et al., 2012, Mol. Biol. Cell 23(12): 2275--2291 Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1. [FBrf0218626] Batiha and Swan, 2012, Genome 55(1): 63--67 Evidence that the spindle assembly checkpoint does not regulate APC(Fzy) activity in Drosophila female meiosis. [FBrf0217126] Delgehyr et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(15): 5729--5734 Drosophila Mgr, a Prefoldin subunit cooperating with von Hippel Lindau to regulate tubulin stability. [FBrf0218047] Herriott et al., 2012, Cell Cycle 11(24): 4650--4660 Kinetochore localized Mad2 and Cdc20 is itself insufficient for triggering the mitotic checkpoint when Mps1 is low in Drosophila melanogaster neuroblasts. [FBrf0220451] Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387 RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643] Shaukat et al., 2012, PLoS ONE 7(10): e47447 A screen for selective killing of cells with chromosomal instability induced by a spindle checkpoint defect. [FBrf0219770] Andrejka et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(42): 17438--17443 Animal-specific C-terminal domain links myeloblastosis oncoprotein (Myb) to an ancient repressor complex. [FBrf0216444] Emre et al., 2011, J. Cell Sci. 124(10): 1664--1671 A mitotic role for Mad1 beyond the spindle checkpoint. [FBrf0213557] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Orr and Sunkel, 2011, Chromosoma 120(1): 83--96 Drosophila CENP-C is essential for centromere identity. [FBrf0212897] Przewloka et al., 2011, Curr. Biol. 21(5): 399--405 CENP-C Is a Structural Platform for Kinetochore Assembly. [FBrf0213151] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...