FlyBase Gene Report: Dmel\mad2

General Information

Symbol

Dmel\mad2

Species

D. melanogaster

Name

mad2

Annotation Symbol

CG17498

Feature Type

protein_coding_gene

FlyBase ID

FBgn0035640

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

mad2 (mad2) encodes a conserved component of the spindle checkpoint. During mitosis, it is recruited to unattached kinetochores, where it binds the products of Mad1 and fzy, promoting the assembly of the mitotic checkpoint complex. During interphase, the product of mad2 is intranuclear, bound to the product of Mad1, and associated primarily with the nuclear pore complex. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

64E11-64E12

Sequence location

3L:5,812,135..5,813,093 [-]

Recombination map

3-15

RefSeq locus

NT_037436 REGION:5812135..5813093

Sequence

Get Decorated FASTA

Genomic Maps

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:A1Z8P9

(assigned by UniProt )

(Lince-Faria et al., 2009)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in chromosome organization

inferred from mutant phenotype

(Wong et al., 2014)

involved_in male germ-line stem cell asymmetric division

inferred from genetic interaction with FLYBASE:Mtor; FB:FBgn0013756

(Liu et al., 2015)

involved_in mitotic spindle assembly checkpoint

inferred from mutant phenotype

(Orr et al., 2007)

inferred from direct assay

(Logarinho et al., 2004)

involved_in regulation of mitotic cell cycle spindle assembly checkpoint

inferred from mutant phenotype

(Buffin et al., 2007)

involved_in regulation of mitotic cell cycle

inferred from mutant phenotype

(Lince-Faria et al., 2009)

involved_in regulation of mitotic spindle assembly

inferred from mutant phenotype

(assigned by UniProt )

(Lince-Faria et al., 2009)

inferred from genetic interaction with FLYBASE:SAK; FB:FBgn0026371

(Basto et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in mitotic spindle assembly checkpoint

inferred from biological aspect of ancestor with PANTHER:PTN000217420

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (10 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Katsani et al., 2008, Buffin et al., 2005)

located_in kinetochore microtubule

inferred from direct assay

(Buffin et al., 2005)

located_in kinetochore

inferred from direct assay

(Lince-Faria et al., 2009, Katsani et al., 2008, Griffis et al., 2007, Buffin et al., 2005, Logarinho et al., 2004)

located_in mitotic spindle

inferred from direct assay

(Lince-Faria et al., 2009, Katsani et al., 2008)

located_in nuclear envelope

inferred from direct assay

(Buffin et al., 2005)

located_in nucleoplasm

inferred from direct assay

(Katsani et al., 2008)

located_in nucleus

inferred from direct assay

(Lince-Faria et al., 2009, Logarinho et al., 2004)

located_in spindle matrix

inferred from direct assay

(Lince-Faria et al., 2009)

located_in spindle pole

inferred from direct assay

(Logarinho et al., 2004)

located_in spindle

inferred from direct assay

(Griffis et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000217420

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in kinetochore

inferred from biological aspect of ancestor with PANTHER:PTN000217420

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000217420

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Summary (Interactive Fly)

mitotic checkpoint protein that blocks mitotic exit by sequestering Cdc20/Fizzy - required to delay progression through early stages of prometaphase so that cells have time to fully engage the spindle assembly checkpoint

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\mad2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.43

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

mad2-RA

FBtr0077113

NM_139734

818

207

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

mad2-PA

FBpp0076819

23.4

207

5.72

NP_647991

AAF50740

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VRQ2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mad2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mad2_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium