FB2025_05 , released December 11, 2025
Gene: Dmel\Vps8
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General Information
Symbol
Dmel\Vps8
Species
D. melanogaster
Name
Vacuolar protein sorting 8
Annotation Symbol
CG10144
Feature Type
protein_coding_gene
FlyBase ID
FBgn0035704
Gene Model Status
Current
Stock Availability
3 publicly available
Gene Summary
Part of the class C core vacuole/endosome tethering (CORVET) complex involved in endo-lysosomal vesicle trafficking and lysosome biogenesis by facilitating docking and fusion of endosomal vesicles (PubMed:27253064, PubMed:31194677). The CORVET complex acts upstream of the homotypic fusion and vacuole protein sorting (HOPS) tethering complex but is not involved in autophagic flux (PubMed:27253064, PubMed:31194677). The CORVET complex may cooperate with the early endosomal tether Rbsn-5 to mediate endosomal fusion (PubMed:27253064). As part of the CORVET complex recruited to endosomes by activated GTP-bound Rab5 (PubMed:27253064). Specifically required for endocytic trafficking in a subset of cells, such as hemocytes and nephrocytes, which are highly active in endocytosis (PubMed:27253064). (UniProt, Q9VRX2)
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
65B3-65B3
Sequence location
Recombination map
3-17
RefSeq locus
NT_037436 REGION:6491230..6495297
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with UniProtKB:A4V4S5,UniProtKB:M9PF72,UniProtKB:Q24314,UniProtKB:Q9VHG1
(Lőrincz et al., 2016)
inferred from physical interaction with UniProtKB:Q24314
(Lőrincz et al., 2016)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables GTPase binding
inferred from sequence or structural similarity with SGD:S000000002
(Solinger and Spang, 2013)
enables protein-membrane adaptor activity
inferred from biological aspect of ancestor with PANTHER:PTN001759852
(GO Reference Genome Project, 2011-)
enables zinc ion binding
inferred from sequence model
(Ying et al., 2011)
Biological Process (7 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in endocytosis
inferred from mutant phenotype
(Lőrincz et al., 2016)
involved_in endosomal vesicle fusion
inferred from direct assay
(Lőrincz et al., 2016)
inferred from genetic interaction with UniProtKB:O76742
(Lőrincz et al., 2016)
involved_in endosome organization
inferred from direct assay
(Lőrincz et al., 2016)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in endosomal transport
inferred from sequence or structural similarity with SGD:S000000002
(Solinger and Spang, 2013)
involved_in endosomal vesicle fusion
inferred from biological aspect of ancestor with PANTHER:PTN001759852
(GO Reference Genome Project, 2011-)
involved_in intracellular protein transport
inferred from electronic annotation with InterPro:IPR000547
(InterPro Project Members, 2004-)
involved_in protein targeting to vacuole
inferred from electronic annotation with InterPro:IPR045111
(InterPro Project Members, 2004-)
involved_in vesicle-mediated transport
inferred from electronic annotation with InterPro:IPR000547
(InterPro Project Members, 2004-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of CORVET complex
inferred from direct assay
(Lőrincz et al., 2016)
located_in early endosome
inferred from direct assay
(Lőrincz et al., 2016)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of CORVET complex
inferred from sequence or structural similarity with SGD:S000000002
(Solinger and Spang, 2013)
inferred from biological aspect of ancestor with PANTHER:PTN002289786
(GO Reference Genome Project, 2011-)
is_active_in late endosome
inferred from biological aspect of ancestor with PANTHER:PTN001759852
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the VPS8 family. (Q9VRX2)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
VPS-C COMPLEXES: HOPS AND CORVET -
The heterohexameric VPS-C complexes, HOPS and CORVET have been extensively characterised in yeast. They interact with SNAREs and Rabs to promote vesicle tethering. The CORVET complex is involved in early endosome fusion, interacting with Rab5-GTP. The HOPS complex interacts with Rab7-GTP on late endosomes (multivesicular bodies) to promote fusion with the lysosomal compartment (and vacuoles) and also autophagosome-lysosome fusion. The VPS-C complexes share common subunits and CORVET to HOPS conversion intermediates (i-CORVET and i-HOPS) have been proposed to accompany early to late endosome maturation. (Adapted from PMID:19575650 and FBrf0223135).
Protein Function (UniProtKB)
Part of the class C core vacuole/endosome tethering (CORVET) complex involved in endo-lysosomal vesicle trafficking and lysosome biogenesis by facilitating docking and fusion of endosomal vesicles (PubMed:27253064, PubMed:31194677). The CORVET complex acts upstream of the homotypic fusion and vacuole protein sorting (HOPS) tethering complex but is not involved in autophagic flux (PubMed:27253064, PubMed:31194677). The CORVET complex may cooperate with the early endosomal tether Rbsn-5 to mediate endosomal fusion (PubMed:27253064). As part of the CORVET complex recruited to endosomes by activated GTP-bound Rab5 (PubMed:27253064). Specifically required for endocytic trafficking in a subset of cells, such as hemocytes and nephrocytes, which are highly active in endocytosis (PubMed:27253064).
(UniProt, Q9VRX2)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Vps8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VRX2)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Vps8-RA
FBtr0076986
NM_139791
3885
1229
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Vps8-PA
FBpp0076695
137.9
1229
6.40
Q9VRX2
NP_648048
AAF50660
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the class C core vacuole/endosome tethering (CORVET) complex composed of at least Vps8, dor/Vps18, car/Vps33A and Vps16A; unlike in other species, Vps11 is not part of the Drosophila complex (PubMed:27253064). Due to the reduced number of components the Drosophila CORVET complex is often referred to as the miniCORVET complex (PubMed:27253064). Has a higher affinity than the homotypic fusion and vacuole protein sorting (HOPS) tethering complex-specific component lt/Vps41 for Vps16A, car/Vps33A and dor/Vps18, the core components shared by both tethering complexes (PubMed:31194677).

(UniProt, Q9VRX2)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Vps8 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.13

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of CORVET complex
inferred from direct assay
(Lőrincz et al., 2016)
located_in early endosome
inferred from direct assay
(Lőrincz et al., 2016)
Expression Deduced from Reporters
Reporter: P{Vps8-HA}
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage -- pupal stage
embryonic/larval garland cell
(Lőrincz et al., 2016)
pericardial cell
(Lőrincz et al., 2016)
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Vps8 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Vps8
Transgenic constructs containing regulatory region of Vps8
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 6 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal eye color, with Scer\GAL4GMR.PF, Scer\GAL4ey.PU
increased cell number | third instar larval stage (with Df(3L)ED211)
increased cell size, with Scer\GAL4pros.PU
increased cell size (with Df(3L)ED211)
increased occurrence of cell division | adult stage, with Scer\GAL4NP0001
majority die during pharate adult stage
majority die during pharate adult stage (with Df(3L)ED211)
melanotic mass phenotype | larval stage (with Df(3L)ED211)
visible (with Df(3L)ED211)
Phenotype manifest in
Allele
adult gut, with Scer\GAL4NP0001
autolysosome | larval stage | somatic clone | calorie restriction conditional, with Scer\GAL4Act.PU
autophagosome | larval stage | somatic clone | calorie restriction conditional, with Scer\GAL4Act.PU
embryonic/larval crystal cell | larval stage | increased number (with Df(3L)ED211)
embryonic/larval fat body | somatic clone | calorie restriction conditional, with Scer\GAL4Act.PU
embryonic/larval garland cell | larval stage
embryonic/larval garland cell | larval stage (with Df(3L)ED211)
embryonic/larval hemocyte | larval stage (with Df(3L)ED211)
embryonic/larval hemocyte | larval stage | increased number (with Df(3L)ED211)
embryonic/larval salivary gland | larval stage, with Scer\GAL4fkh.PH
endolysosome | larval stage (with Df(3L)ED211)
eye, with Scer\GAL4GMR.PF, Scer\GAL4ey.PU
garland cell, with Scer\GAL4pros.PU
garland cell (with Df(3L)ED211)
lamellocyte | larval stage | increased number (with Df(3L)ED211)
larval sessile hemocyte | larval stage (with Df(3L)ED211)
late endosome, with Scer\GAL4pros.PU
late endosome (with Df(3L)ED211)
late endosome | larval stage
late endosome | larval stage (with Df(3L)ED211)
lysosome | larval stage, with Scer\GAL4fkh.PH
lysosome | larval stage | somatic clone | calorie restriction conditional, with Scer\GAL4Act.PU
vacuole | larval stage (with Df(3L)ED211)
wing (with Df(3L)ED211)
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\VPS8
13 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Vps8
12 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Vps8
12 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\vps8
11 of 13
Yes
Yes
Xtro\mypop
1 of 13
No
Yes
Danio rerio (Zebrafish) (1)
Drer\vps8
14 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\vps-8
13 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP007578
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\SETH5
13 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\VPS8
11 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\vps8
7 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Vps8. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (2)
lt
1 of 13
Rbbp5
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    VPS8; VPS8 subunit of CORVET complex
    13 of 14
    618366
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Vps8
        enhanceable
        lt
        (Lőrincz et al., 2016)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Component of the class C core vacuole/endosome tethering (CORVET) complex composed of at least Vps8, dor/Vps18, car/Vps33A and Vps16A; unlike in other species, Vps11 is not part of the Drosophila complex (PubMed:27253064). Due to the reduced number of components the Drosophila CORVET complex is often referred to as the miniCORVET complex (PubMed:27253064). Has a higher affinity than the homotypic fusion and vacuole protein sorting (HOPS) tethering complex-specific component lt/Vps41 for Vps16A, car/Vps33A and dor/Vps18, the core components shared by both tethering complexes (PubMed:31194677).
        (UniProt, Q9VRX2 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3L
        Recombination map
        3-17
        Cytogenetic map
        65B3-65B3
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        65B3-65B3
        Limits computationally determined from genome sequence between P{PZ}l(3)0209402094 and P{lacW}l(3)L4060L4060
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        3-17

        (FlyBase, 2016)

        3-14.8

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (3)
        Genomic Clones (19)
        cDNA Clones (44)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments

              Area matching Drosophila EST AI064638 (inverted).

              (Davies, 2001.3.15)
              Relationship to Other Genes
              Source for database merge of

              Source for merge of: CG10144 anon-WO0118547.365

              (FlyBase, 1996-)
              Additional comments

              Source for merge of CG10144 anon-WO0118547.365 was sequence comparison ( date:051113 ).

              (FlyBase, 1996-)
              Nomenclature History
              Source for database identify of

              Source for identity of: Vps8 CG10144

              (Li and Blissard, 2015)
              Nomenclature comments
              Etymology
              Synonyms and Secondary IDs (5)
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              Study result (0)
              Result
              Result Type
              Title
              External Crossreferences and Linkouts ( 37 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              Other crossreferences
              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
              FlyMine - An integrated database for Drosophila genomics
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              MARRVEL_MODEL - MARRVEL (model organism gene)
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
              MIST (genetic) - An integrated Molecular Interaction Database
              MIST (protein-protein) - An integrated Molecular Interaction Database
              References (45)