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Gene Dmel\Mis12

General Information
Symbol	Dmel\Mis12	Species	D. melanogaster
Name	Mis12	Annotation symbol	CG18156
Feature type	protein_coding_gene	FlyBase ID	FBgn0035725
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	dmMis12
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	65D5-65D5	Sequence location	3L:6,961,135..6,962,148 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Mis12 is referred to in FlyBase by the symbol Dmel\Mis12 (CG18156, FBgn0035725). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: mitotic metaphase plate congression; mitotic anaphase; chromosome segregation; mitotic spindle organization; neurogenesis. 14 alleles are reported. The phenotypes of these alleles are annotated with: metaphase; condensed nuclear chromosome; spindle; mesothoracic tergum; nucleus. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1633850_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Mis12. Gene sequence location is 3L:6961135..6962148.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 65D5-65D5   Limits computationally determined from genome sequence between P{lacW}l(3)L4060L4060 and P{PZ}corn04202
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Mis12 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Mis12-RA FBtr0076936  NM_139809   839   181
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Mis12-PA   FBpp0076645   21.1   181   5.98     NP_648066   AAF50629
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF50629   AY071159 AAL48781   BI213906   CO183604   CO193631   CZ486342   CZ486343
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VS01
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
epitope tag Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 5   cell   • kinetochore (Schittenhelm et al., 2007) Comment:Localization observed prometaphase until late anaphase of embryonic cycle 14   cell   • kinetochore (Schittenhelm et al., 2007) Comment:Localization observed in mitotic cells; assayed in cultured S2R+ cells
Additional Descriptive Data
	Subcellular localization to kinetochores of embryonic mitotic cells assayed using the transgenic allele Mis12T:Avic\GFP-EGFP, and of cultured S2R+ cells using the transgenic allele Mis12Scer\UAS.T:Avic\GFP-EGFP. (Schittenhelm et al., 2007)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
chromosome, centromeric region inferred from direct assay (Venkei et al., 2011) kinetochore inferred from direct assay (Przewloka et al., 2007, Schittenhelm et al., 2007, Venkei et al., 2011) colocalizes_with kinetochore inferred from direct assay (Goshima et al., 2007) Ndc80 complex inferred from direct assay (Schittenhelm et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    44.8 Larval Midgut    15.2 Larval Hindgut    16.9 Larval Malpighian Tubules    27.3 Larval Fat Body    33.8 Larval Salivary Gland    21.3 Larval Trachea    29.7 Larval Carcass    18.975 Adult Head    36.4 Adult Eye    33.8 Adult Brain    101.4 Adult Thoracic-Abdominal Ganglion    132 Adult Crop    22.5 Adult Midgut    14.2 Adult Hindgut    16.3 Adult Malpighian Tubules    11.4 Adult Fat Body    12.4 Adult Salivary Gland    14.5 Adult Heart    15.25 Adult VirginFemale Spermatheca    23 Adult InseminatedFemale Spermatheca    23.4 Adult Ovary    211.7 Adult Testis    79.5 Adult Male Accessory Gland    18.6 Adult Carcass    20.1 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    44.8 Larval Midgut    15.2 Larval Hindgut    16.9 Larval Malpighian Tubules    27.3 Larval Fat Body    33.8 Larval Salivary Gland    21.3 Larval Trachea    29.7 Larval Carcass    18.975 Adult Head    36.4 Adult Eye    33.8 Adult Brain    101.4 Adult Thoracic-Abdominal Ganglion    132 Adult Crop    22.5 Adult Midgut    14.2 Adult Hindgut    16.3 Adult Malpighian Tubules    11.4 Adult Fat Body    12.4 Adult Salivary Gland    14.5 Adult Heart    15.25 Adult VirginFemale Spermatheca    23 Adult InseminatedFemale Spermatheca    23.4 Adult Ovary    211.7 Adult Testis    79.5 Adult Male Accessory Gland    18.6 Adult Carcass    20.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    44.8 Larval Midgut    15.2 Larval Hindgut    16.9 Larval Malpighian Tubules    27.3 Larval Fat Body    33.8 Larval Salivary Gland    21.3 Larval Trachea    29.7 Larval Carcass    18.975 Adult Head    36.4 Adult Eye    33.8 Adult Brain    101.4 Adult Thoracic-Abdominal Ganglion    132 Adult Crop    22.5 Adult Midgut    14.2 Adult Hindgut    16.3 Adult Malpighian Tubules    11.4 Adult Fat Body    12.4 Adult Salivary Gland    14.5 Adult Heart    15.25 Adult VirginFemale Spermatheca    23 Adult InseminatedFemale Spermatheca    23.4 Adult Ovary    211.7 Adult Testis    79.5 Adult Male Accessory Gland    18.6 Adult Carcass    20.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    44.8 Larval Midgut    15.2 Larval Hindgut    16.9 Larval Malpighian Tubules    27.3 Larval Fat Body    33.8 Larval Salivary Gland    21.3 Larval Trachea    29.7 Larval Carcass    18.975 Adult Head    36.4 Adult Eye    33.8 Adult Brain    101.4 Adult Thoracic-Abdominal Ganglion    132 Adult Crop    22.5 Adult Midgut    14.2 Adult Hindgut    16.3 Adult Malpighian Tubules    11.4 Adult Fat Body    12.4 Adult Salivary Gland    14.5 Adult Heart    15.25 Adult VirginFemale Spermatheca    23 Adult InseminatedFemale Spermatheca    23.4 Adult Ovary    211.7 Adult Testis    79.5 Adult Male Accessory Gland    18.6 Adult Carcass    20.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    44.8 Larval Midgut    15.2 Larval Hindgut    16.9 Larval Malpighian Tubules    27.3 Larval Fat Body    33.8 Larval Salivary Gland    21.3 Larval Trachea    29.7 Larval Carcass    18.975 Adult Head    36.4 Adult Eye    33.8 Adult Brain    101.4 Adult Thoracic-Abdominal Ganglion    132 Adult Crop    22.5 Adult Midgut    14.2 Adult Hindgut    16.3 Adult Malpighian Tubules    11.4 Adult Fat Body    12.4 Adult Salivary Gland    14.5 Adult Heart    15.25 Adult VirginFemale Spermatheca    23 Adult InseminatedFemale Spermatheca    23.4 Adult Ovary    211.7 Adult Testis    79.5 Adult Male Accessory Gland    18.6 Adult Carcass    20.1 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    44.8 Larval Midgut    15.2 Larval Hindgut    16.9 Larval Malpighian Tubules    27.3 Larval Fat Body    33.8 Larval Salivary Gland    21.3 Larval Trachea    29.7 Larval Carcass    18.975 Adult Head    36.4 Adult Eye    33.8 Adult Brain    101.4 Adult Thoracic-Abdominal Ganglion    132 Adult Crop    22.5 Adult Midgut    14.2 Adult Hindgut    16.3 Adult Malpighian Tubules    11.4 Adult Fat Body    12.4 Adult Salivary Gland    14.5 Adult Heart    15.25 Adult VirginFemale Spermatheca    23 Adult InseminatedFemale Spermatheca    23.4 Adult Ovary    211.7 Adult Testis    79.5 Adult Male Accessory Gland    18.6 Adult Carcass    20.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    44.8 Larval Midgut    15.2 Larval Hindgut    16.9 Larval Malpighian Tubules    27.3 Larval Fat Body    33.8 Larval Salivary Gland    21.3 Larval Trachea    29.7 Larval Carcass    18.975 Adult Head    36.4 Adult Eye    33.8 Adult Brain    101.4 Adult Thoracic-Abdominal Ganglion    132 Adult Crop    22.5 Adult Midgut    14.2 Adult Hindgut    16.3 Adult Malpighian Tubules    11.4 Adult Fat Body    12.4 Adult Salivary Gland    14.5 Adult Heart    15.25 Adult VirginFemale Spermatheca    23 Adult InseminatedFemale Spermatheca    23.4 Adult Ovary    211.7 Adult Testis    79.5 Adult Male Accessory Gland    18.6 Adult Carcass    20.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    44.8 Larval Midgut    15.2 Larval Hindgut    16.9 Larval Malpighian Tubules    27.3 Larval Fat Body    33.8 Larval Salivary Gland    21.3 Larval Trachea    29.7 Larval Carcass    18.975 Adult Head    36.4 Adult Eye    33.8 Adult Brain    101.4 Adult Thoracic-Abdominal Ganglion    132 Adult Crop    22.5 Adult Midgut    14.2 Adult Hindgut    16.3 Adult Malpighian Tubules    11.4 Adult Fat Body    12.4 Adult Salivary Gland    14.5 Adult Heart    15.25 Adult VirginFemale Spermatheca    23 Adult InseminatedFemale Spermatheca    23.4 Adult Ovary    211.7 Adult Testis    79.5 Adult Male Accessory Gland    18.6 Adult Carcass    20.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    36.4   NA  Eye    33.8   NA  Brain    101.4   44.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    132   NA  Crop    no informative data   no informative data  Midgut    no informative data   no informative data  Hindgut    no informative data   27.3  Malpighian Tubules    no informative data   no informative data  Fat Body    no informative data   21.3  Salivary Gland    no informative data   NA  Heart    no informative data   29.7  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    23.4   NA  Ovary    211.7   NA  Testis    79.5   NA  Male Accessory Gland    no informative data   no informative data  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    8 central nervous system, pupae P8    14 head, virgin 1-day female    5 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    14 head, mated 20-day male    18 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    0 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    17 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    8 central nervous system, pupae P8    14 head, virgin 1-day female    5 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    14 head, mated 20-day male    18 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    0 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    17 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    8 central nervous system, pupae P8    14 head, virgin 1-day female    5 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    14 head, mated 20-day male    18 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    0 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    17 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    8 central nervous system, pupae P8    14 head, virgin 1-day female    5 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    14 head, mated 20-day male    18 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    0 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    17 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    8 central nervous system, pupae P8    14 head, virgin 1-day female    5 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    14 head, mated 20-day male    18 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    0 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    17 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    8 central nervous system, pupae P8    14 head, virgin 1-day female    5 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    14 head, mated 20-day male    18 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    0 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    17 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    8 central nervous system, pupae P8    14 head, virgin 1-day female    5 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    14 head, mated 20-day male    18 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    0 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    17 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    8 central nervous system, pupae P8    14 head, virgin 1-day female    5 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    14 head, mated 20-day male    18 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    0 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    17 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    8 embryo 04-06hr    18 embryo 06-08hr    15 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    4 adult male 05day    5 adult male 30day    4 adult female 01day    5 adult female 05day    12 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    8 embryo 04-06hr    18 embryo 06-08hr    15 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    4 adult male 05day    5 adult male 30day    4 adult female 01day    5 adult female 05day    12 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    8 embryo 04-06hr    18 embryo 06-08hr    15 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    4 adult male 05day    5 adult male 30day    4 adult female 01day    5 adult female 05day    12 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    8 embryo 04-06hr    18 embryo 06-08hr    15 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    4 adult male 05day    5 adult male 30day    4 adult female 01day    5 adult female 05day    12 adult female 30day    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    8 embryo 04-06hr    18 embryo 06-08hr    15 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    4 adult male 05day    5 adult male 30day    4 adult female 01day    5 adult female 05day    12 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    8 embryo 04-06hr    18 embryo 06-08hr    15 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    4 adult male 05day    5 adult male 30day    4 adult female 01day    5 adult female 05day    12 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    8 embryo 04-06hr    18 embryo 06-08hr    15 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    4 adult male 05day    5 adult male 30day    4 adult female 01day    5 adult female 05day    12 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    8 embryo 04-06hr    18 embryo 06-08hr    15 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    3 pupae 4d postWPP    2 adult male 01day    4 adult male 05day    5 adult male 30day    4 adult female 01day    5 adult female 05day    12 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    19 embryonic 1182-4H    11 embryonic GM2    6 embryonic Kc167    19 embryonic S1    16 embryonic S3    17 leg disc CME L1    12 wing disc CME-W2    12 wing disc ML-DmD8    8 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    31 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    35 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    35 ovary fGS/OSS    23 ovary OSC    8 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (47) Schneider line 2 Sg4    19 embryonic 1182-4H    11 embryonic GM2    6 embryonic Kc167    19 embryonic S1    16 embryonic S3    17 leg disc CME L1    12 wing disc CME-W2    12 wing disc ML-DmD8    8 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21  (31) wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1  (35) CNS ML-DmBG2-c2    17 tumorous blood cells mbn2  (35) ovary fGS/OSS    23 ovary OSC    8 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    19 embryonic 1182-4H    11 embryonic GM2    6 embryonic Kc167    19 embryonic S1    16 embryonic S3    17 leg disc CME L1    12 wing disc CME-W2    12 wing disc ML-DmD8    8 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    31 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    35 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    35 ovary fGS/OSS    23 ovary OSC    8 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    19 embryonic 1182-4H    11 embryonic GM2    6 embryonic Kc167    19 embryonic S1    16 embryonic S3    17 leg disc CME L1    12 wing disc CME-W2    12 wing disc ML-DmD8    8 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    31 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    35 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    35 ovary fGS/OSS    23 ovary OSC    8 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    19 embryonic 1182-4H    11 embryonic GM2    6 embryonic Kc167    19 embryonic S1    16 embryonic S3    17 leg disc CME L1    12 wing disc CME-W2    12 wing disc ML-DmD8    8 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    31 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    35 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    35 ovary fGS/OSS    23 ovary OSC    8 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (47) Schneider line 2 Sg4    19 embryonic 1182-4H    11 embryonic GM2    6 embryonic Kc167    19 embryonic S1    16 embryonic S3    17 leg disc CME L1    12 wing disc CME-W2    12 wing disc ML-DmD8    8 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21  31 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1  (35) CNS ML-DmBG2-c2    17 tumorous blood cells mbn2  (35) ovary fGS/OSS    23 ovary OSC    8 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    19 embryonic 1182-4H    11 embryonic GM2    6 embryonic Kc167    19 embryonic S1    16 embryonic S3    17 leg disc CME L1    12 wing disc CME-W2    12 wing disc ML-DmD8    8 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    31 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    35 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    35 ovary fGS/OSS    23 ovary OSC    8 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    19 embryonic 1182-4H    11 embryonic GM2    6 embryonic Kc167    19 embryonic S1    16 embryonic S3    17 leg disc CME L1    12 wing disc CME-W2    12 wing disc ML-DmD8    8 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    31 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    35 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    35 ovary fGS/OSS    23 ovary OSC    8 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    13 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • RE19545 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035725 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18156-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Mis12GD8621 lethal (with Df(3L)BSC27) Mis12f03756 lethal | embryonic stage (with Df(3L)BSC224) Mis12f03756 lethal | embryonic stage | recessive Mis12f03756 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Mis12GD8621 Other Phenotypes Allele mitotic cell cycle defective Mis12dsRNA.cPa mitotic cell cycle defective | embryonic stage Mis12f03756 neuroanatomy defective | larval stage Mis12GD8621 visible, with Scer\GAL4pnr-MD237 Mis12GD8621 Phenotype manifest in Allele condensed nuclear chromosome Mis12dsRNA.cPa embryonic/larval neuroblast | larval stage Mis12GD8621 ganglion mother cell | larval stage Mis12GD8621 mesothoracic tergum, with Scer\GAL4pnr-MD237 Mis12GD8621 metaphase Mis12dsRNA.cPa nucleus Mis12dsRNA.cPa spindle Mis12dsRNA.cPa
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Mis12 Class Mutagen Stocks Known lesion Mis12f03756 piggyBac transposase 2 Yes
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Mis12 Class Mutagen Stocks Known lesion Mis12GD8621 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Mis12GL00399 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mis12HMS01550 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mis12HMS01753 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mis12KK113187 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mis12Act5C.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Mis12Act5C.T:Saur\A in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Mis12Act5C.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Mis12dsRNA.cPa in vitro construct - RNAi 0 Yes Mis12Scer\UAS.P\T.C.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Mis12Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Mis12T:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes Mis12T:Disc\RFP-mCherry in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(3L)vvl7247-Mis12 (Sotillos et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD8621} NA P{KK113187} NA P{TRiP.GL00399} NA P{TRiP.HMS01550} NA P{TRiP.HMS01753} NA P{UASp-Mis12-EGFP} NA characterization construct P{gMis12-EGFP} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}Mis12f03756 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
chromosome segregation inferred from mutant phenotype (Przewloka et al., 2007) mitotic anaphase inferred from mutant phenotype (Venkei et al., 2011) mitotic metaphase plate congression inferred from mutant phenotype (Przewloka et al., 2007) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
chromosome, centromeric region inferred from direct assay (Venkei et al., 2011) kinetochore inferred from direct assay (Przewloka et al., 2007, Schittenhelm et al., 2007, Venkei et al., 2011) colocalizes_with kinetochore inferred from direct assay (Goshima et al., 2007) Ndc80 complex inferred from direct assay (Schittenhelm et al., 2007)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Mis12 - Arc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mis12 - CG1532 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mis12 - CG7049 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mis12 - Kmn1 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Mis12 - Nnf1a anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Mis12 - Nnf1b experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Mis12 - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mis12 - RpS11 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mis12 - Tm1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Mis12 allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0035725 DroID - A comprehensive database of gene and protein interactions. • FBgn0035725
Orthologs
OrthoDB Orthologs (12) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6NGH76 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6NGH76)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Mis12     Drosophila simulans   Dsim\GD13101 Y   Drosophila sechellia   Dsec\GM13802 Y   Drosophila erecta   Dere\GG15013 Y   Drosophila yakuba   Dyak\GE20456 Y   Drosophila ananassae   Dana\GF14880 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA25888 Y   Drosophila persimilis   Dper\GL19253 Y   Drosophila willistoni   Dwil\GK24429 Y   Drosophila virilis   Dvir\GJ19698 Y   Drosophila mojavensis   Dmoj\GI18024 Y   Drosophila grimshawi   Dgri\GH10506 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD13101 Drosophila sechellia Dsec\GM13802 Drosophila erecta Dere\GG15013 Drosophila yakuba Dyak\GE20456 Drosophila ananassae Dana\GF14880 Drosophila pseudoobscura pseudoobscura Dpse\GA25888 Drosophila persimilis Dper\GL19253 Drosophila willistoni Dwil\GK24429 Drosophila virilis Dvir\GJ19698 Drosophila mojavensis Dmoj\GI18024 Drosophila grimshawi Dgri\GH10506
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	35471 y1 sc* v1; P{TRiP.GL00399}attP2 38535 y1 sc* v1; P{TRiP.HMS01753}attP2 36662 y1 sc* v1; P{TRiP.HMS01550}attP40 18687 w1118; PBac{WH}Mis12f03756/TM6B, Tb1
Harvard	- PBac{WH}Mis12f03756
VDRC	v105052 P{KK113187}VIE-260B v19097 w1118; P{GD8621}v19097 v19098 w1118; P{GD8621}v19098
Genomic Clones ( 1 )
	BACR14L10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 4 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE19545
Other clones	DPiM_RE19545
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	EC26022 EC36521
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0035725 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38762
Antibody Information
	polyclonal (Venkei et al., 2011)
Other Information
Discoverer
Etymology
Identification
	On the basis of sequence similarity to the human MIS12 gene (OMIM:609178). (Przewloka et al., 2007)
Relationship to Other Genes
Source for database identity of	Source for identity of: Mis12 CG18156 (Schittenhelm et al., 2007, Przewloka et al., 2007, Goshima et al., 2007)
Source for database merge of	Source for merge of: CG18156 BcDNA:RE19545 (FlyBase, 1996-)
Additional comments	FlyBase curator comment: Correspondence with author Lehner revealed that 'Mis12' = 'CG18156'. (Lehner et al., 2007) Source for merge of CG18156 BcDNA:RE19545 was a shared cDNA (date:020730). (FlyBase, 1996-)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle and a spindle that is aberrantly long when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_139809 RefSeq (Proteins) • NP_648066 DDBJ / EMBL / GenBank • AAF50629 AAL48781 AY071159 BI213906 CZ486342 CZ486343 Entrez Gene - A searchable database of RefSeq genes. • 38762 UniProtKB/TrEMBL • Q9VS01
Other Crossreferences
	BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE19545
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0035725 DroID - A comprehensive database of gene and protein interactions. • FBgn0035725 DRSC - Results from RNAi screens. • FBgn0035725 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035725 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18156-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0035725 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38762 modMine - Data generated by the modENCODE project. • FBgn0035725
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	BcDNA:RE19545   CG18156 (Goshima et al., 2007, Schittenhelm et al., 2007, Przewloka et al., 2007, Goshima et al., 2007, Keleman et al., 2009.8.5, Ni et al., 2011.7.19, Ni et al., 2010.12.1) dmMis12 (Tavares et al., 2007, Przewloka et al., 2007) Mis12 (Lehner et al., 2007, Griffis et al., 2007, Schittenhelm et al., 2009, Schittenhelm et al., 2007, Goshima et al., 2007, Goshima et al., 2008, Schittenhelm et al., 2010, Venkei et al., 2011, Przewloka et al., 2011, Neumüller et al., 2011) Mis-12 (Williams et al., 2007)
Name Synonym	CG18156   Mis12
Secondary FlyBase IDs
	FBgn0063053
References ( 35 )
Generate a list of	All references relating to this gene ( 35 )
List References by type	Research paper  ( 15 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) Website  ( 1 )
Recent research papers ( 4 )
	Venkei et al., 2012, Open Biol. 2(2): 110032 Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore. [FBrf0218508] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Przewloka et al., 2011, Curr. Biol. 21(5): 399--405 CENP-C Is a Structural Platform for Kinetochore Assembly. [FBrf0213151] Venkei et al., 2011, Genetics 187(1): 131--140 Drosophila mis12 complex acts as a single functional unit essential for anaphase chromosome movement and a robust spindle assembly checkpoint. [FBrf0212773] Show all research papers ...