FlyBase Gene Report: Dmel\Tsp66A

General Information

Symbol

Dmel\Tsp66A

Species

D. melanogaster

Name

Tetraspanin 66A

Annotation Symbol

CG16991

Feature Type

protein_coding_gene

FlyBase ID

FBgn0035786

Gene Model Status

Current

Stock Availability

2 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

66A2-66A2

Sequence location

3L:7,465,509..7,468,808 [+]

Recombination map

3-21

RefSeq locus

NT_037436 REGION:7465509..7468808

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR008952, InterPro:IPR018499

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity

(Todres et al., 2000)

inferred from sequence model

(Fradkin et al., 2002)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001060286

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

TETRASPANINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

TETRASPANINS -: Tetraspanins (TSP) have four transmembrane domains and two extracellular loops containing specific short motifs, including highly conserved cysteine residues. TSPs are thought to function as scaffolds that bring multiple proteins - including other TSPs, membrane-associated proteins, extracellular and cytosolic proteins - into single membrane region. These regions are called tetraspanin-enriched microdomains. (Adapted from FBrf0227776 and FBrf0152316).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Tsp66A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry M9PEE6)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Tsp66A-RC

FBtr0299721

NM_168216

1308

236

Tsp66A-RD

FBtr0302557

NM_001202134

2531

236

Tsp66A-RE

FBtr0332798

NM_001274589

1314

238

Tsp66A-RF

FBtr0332799

NM_001274590

1302

234

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Tsp66A-PC

FBpp0288999

27.6

236

8.14

FBpp0291713

27.6

236

8.14

FBpp0305021

27.8

238

8.14

FBpp0305022

27.4

234

8.19

M9PEV5

NP_001261519

AGB94214

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

236 aa isoforms: Tsp66A-PC, Tsp66A-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tsp66A using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.24

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Tsp66A in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Tsp66A

View exonic expression by tissue for Dmel\Tsp66A

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035786

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38836/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035786

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035786

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035786

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG16991

Flygut - An atlas of the Drosophila adult midgut

FBgn0035786

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Tsp66A

Transgenic constructs containing regulatory region of Tsp66A

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel8104

(Ryder, 2004.4.26)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (31)

Hsap\CD53

2 of 14

Yes

Hsap\CD63

2 of 14

Yes

Hsap\CD81

2 of 14

Yes

Hsap\CD151

2 of 14

Yes

Hsap\TSPAN2

2 of 14

Yes

Hsap\TSPAN3

2 of 14

Yes

Hsap\TSPAN4

2 of 14

Yes

Hsap\TSPAN5

2 of 14

Yes

Hsap\TSPAN6

2 of 14

Yes

Hsap\TSPAN7

2 of 14

Yes

Hsap\TSPAN9

2 of 14

Yes

Hsap\TSPAN11

2 of 14

Yes

Hsap\TSPAN15

2 of 14

Yes

Hsap\TSPAN17

2 of 14

Yes

Hsap\TSPAN19

2 of 14

Yes

Hsap\TSPAN33

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (26)

Rnor\Cd37

2 of 14

Yes

Rnor\Cd53

2 of 14

Yes

Rnor\Cd63

2 of 14

Yes

Rnor\Cd81

2 of 14

Yes

Rnor\Tspan6

2 of 14

Yes

Rnor\Tspan15

2 of 14

Yes

Rnor\Tspan33

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mus musculus (laboratory mouse) (29)

Mmus\Cd37

2 of 14

Yes

Mmus\Cd53

2 of 14

Yes

Mmus\Cd63

2 of 14

Yes

Mmus\Cd81

2 of 14

Yes

Mmus\Tspan6

2 of 14

Yes

Mmus\Tspan7

2 of 14

Yes

Mmus\Tspan11

2 of 14

Yes

Mmus\Tspan15

2 of 14

Yes

Mmus\Tspan33

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\cd53

1 of 13

Yes

Xtro\cd81

1 of 13

Yes

Xtro\pds5a

1 of 13

Yes

Xtro\slc25a12

1 of 13

Yes

Xtro\stam

1 of 13

Yes

Xtro\tspan3

1 of 13

Yes

Xtro\tspan5

1 of 13

Yes

Xtro\tspan7

1 of 13

Yes

Xtro\tspan9

1 of 13

Yes

Xtro\tspan14

1 of 13

Yes

Xtro\zmym4

1 of 13

Yes

Danio rerio (Zebrafish) (45)

Drer\cd63

2 of 14

Yes

Drer\cd151

2 of 14

Yes

Drer\cd151l

2 of 14

Yes

Drer\tspan4b

2 of 14

Yes

Drer\tspan7b

2 of 14

Yes

Drer\tspan7

2 of 14

Yes

Drer\tspan9a

2 of 14

Yes

Drer\tspan15

2 of 14

Yes

Drer\tspan36

2 of 14

Yes

Drer\upk1a

2 of 14

Yes

Drer\zgc:113223

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (17)

Cele\tsp-6

2 of 14

Yes

Cele\tsp-7

2 of 14

Yes

Cele\tsp-8

2 of 14

Yes

Cele\tsp-11

2 of 14

Yes

Cele\tsp-13

2 of 14

Yes

Cele\tsp-21

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (16)

Agam\AgaP_AGAP000823

2 of 12

Yes

Agam\AgaP_AGAP002257

2 of 12

Yes

Agam\AgaP_AGAP004861

2 of 12

Yes

Agam\AgaP_AGAP005233

2 of 12

Yes

Agam\AgaP_AGAP007209

2 of 12

Yes

Agam\AgaP_AGAP007210

2 of 12

Yes

Agam\AgaP_AGAP008527

2 of 12

Yes

Agam\AgaP_AGAP008886

2 of 12

Yes

Agam\AgaP_AGAP010598

2 of 12

Yes

Agam\AgaP_AGAP011342

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Tsp66A. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (35)

CG30160

3 of 13

lbm

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Tsp66A - Hsp23

experimental knowledge based

(Guruharsha et al., 2011)

Tsp66A - Hsp26

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0035786

MIST (protein-protein) - An integrated Molecular Interaction Database

38836

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_polycistronic_transcript

(FlyBase Genome Annotators, 2010)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-21

Cytogenetic map

66A2-66A2

Sequence location

3L:7,465,509..7,468,808 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66A2-66A2

Limits computationally determined from genome sequence between P{PZ}l(3)07217⁰⁸²²³&P{EP}Rac2^EP3118 and P{lacW}Nmt^j1C7

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-21

(FlyBase, 2016)

3-17.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

35931

y¹ w^*; Mi{MIC}CG42660^MI00661 Tsp66A^MI00661 CG42661^MI00661

FlyORF

F003705

M{UAS-Tsp66A.ORF.3xHA.GW}ZH-86Fb

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP08473

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0035786

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LP21452

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0035786

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

One or more of the processed transcripts for these genes contain two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by Tsp66A (CG16991, FBgn0035786), CG42660 (FBgn0261536), CG42661 (FBgn0261537).

(FlyBase Genome Annotators, 2010)

Nomenclature History

Source for database identify of

Source for identity of: Tsp66A CG16991

(Todres et al., 2000)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG16991

(Metaxakis et al., 2005, Fradkin et al., 2002, Todres et al., 2000)

Dm.Tsp66A

(Todres et al., 2000)

Tsp66A

(Xu et al., 2019, FlyBase Genome Annotators, 2010, Fradkin et al., 2002)

Name Synonyms

Tetraspanin 66A

(Todres et al., 2000)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38836

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

M9PEE6

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

M9PEE6

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035786

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38836/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035786

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035786

FlyMine - An integrated database for Drosophila genomics

FBgn0035786

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG16991

MARRVEL_MODEL - MARRVEL (model organism gene)

38836

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0035786

DroID - A comprehensive database of gene and protein interactions.

FBgn0035786

DRSC - Results frm RNAi screens

FBgn0035786

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035786

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0035786

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG16991

Flygut - An atlas of the Drosophila adult midgut

FBgn0035786

MIST (protein-protein) - An integrated Molecular Interaction Database

38836

References (22)

The following external sites may use different assemblies or annotations than FlyBase.

2.24

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