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Gene Dmel\Atg18

General Information
Symbol	Dmel\Atg18	Species	D. melanogaster
Name	Autophagy-specific gene 18	Annotation symbol	CG7986
Feature type	protein_coding_gene	FlyBase ID	FBgn0035850
Gene Model Status	Current	Stock availability	7 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	66B11-66B11	Sequence location	3L:8,180,095..8,184,065 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Autophagy-specific gene 18 is referred to in FlyBase by the symbol Dmel\Atg18 (CG7986, FBgn0035850). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: larval midgut histolysis; macroautophagy; regulation of defense response to virus; regulation of autophagy; autophagy. 9 alleles are reported. The phenotypes of these alleles are annotated with: NMJ bouton; embryonic/larval salivary gland; neuromuscular junction. It has 6 annotated transcripts and 6 annotated polypeptides. Protein features are: WD40 repeat; WD40-repeat-containing domain; WD40/YVTN repeat-like-containing domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval Malpighian tubules, larval fat body, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1639602_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Atg18. Gene sequence location is 3L:8180095..8184065.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms phosphatidylinositol-3,5-bisphosphate binding cytosol phosphatidylinositol-3-phosphate binding pre-autophagosomal structure membrane cytoplasm autophagic vacuole assembly autophagic vacuole membrane
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 66B11-66B11   Limits computationally determined from genome sequence between P{lacW}Nmtj1C7 and P{lacW}l(3)L0139L0139
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Atg18 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.45 Low-frequency RNA-seq exon junction(s) not annotated. Annotated transcripts do not represent all supported alternative splices within 5' UTR. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Atg18-RA FBtr0076691  NM_139927   2097   377 Atg18-RB FBtr0076692  NM_168259   2382   377 Atg18-RC FBtr0076693  NM_206301   2373   377 Atg18-RD FBtr0321319  NM_001274635   1980   435 Atg18-RE FBtr0321320  NM_001274636   2267   447 Atg18-RF FBtr0321321  NM_001274637   2291   372
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Atg18-PA   FBpp0076414   41.7   377   7.06     NP_648184   AAF50471 Atg18-PB   FBpp0076415   41.7   377   7.06     NP_729341   AAF50472 Atg18-PC   FBpp0076416   41.7   377   7.06     NP_996023   AAS65056 Atg18-PD   FBpp0302862   46.5   435   6.73     NP_001261564   AGB94259 Atg18-PE   FBpp0302863   47.9   447   6.73     NP_001261565   AGB94260 Atg18-PF   FBpp0302864   41.0   372   6.88     NP_001261566   AGB94261
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • WD40-repeat-containing domain (IPR017986) WD40 repeat (IPR001680) WD40/YVTN repeat-like-containing domain (IPR015943)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201999   AA392911   AA438837   AA536360   AA538889   AA802930   AA951793   AE014296 AAF50471   AE014296 AAF50472   AE014296 AAS65056   AE014296 AGB94259   AE014296 AGB94260   AE014296 AGB94261   AI113865   AI257528   AI257739   AI259787   AI293671   AI456857   AI513203   AI513597   AI515267   AI519194   AW943438   AY051899 AAK93323   BH809427   BI162678   BI162679   BI170129   BI172210   BI215401   BI357576   BI370520   BI635903   BQ103696   CK445266   CK659822   CO177250   CO177764   CO266125   CO266821   CO266875   CO275056   CO281172   CO295876   CO296315   CO298218   CO303009   CO304379   CO307309   CO313518   CO322610   CO330652   CO333147   EC058186   EC061491   EC065277   EC073217   EC082780   EC082880   EC084566   EC090366   EC202730   EC214893   EC222309   EC236172   EC236342   EC246417   EC251217   EC257837
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VSF0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   central brain anlage in statu nascendi   (Fisher et al., 2012) dorsal ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi ventral ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi antennal anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal ectoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval Malpighian tubules, larval fat body, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    218.575 Larval Midgut    438.1 Larval Hindgut    277.6 Larval Malpighian Tubules    540.9 Larval Fat Body    744.3 Larval Salivary Gland    302 Larval Trachea    377.55 Larval Carcass    320.4 Adult Head    227.9 Adult Eye    200.375 Adult Brain    236.3 Adult Thoracic-Abdominal Ganglion    185.5 Adult Crop    327.2 Adult Midgut    334.9 Adult Hindgut    261.1 Adult Malpighian Tubules    467 Adult Fat Body    388.7 Adult Salivary Gland    310.7 Adult Heart    406.15 Adult VirginFemale Spermatheca    880.7 Adult InseminatedFemale Spermatheca    700.2 Adult Ovary    308.5 Adult Testis    71.5 Adult Male Accessory Gland    335.4 Adult Carcass    342.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    218.575 Larval Midgut    438.1 Larval Hindgut    277.6 Larval Malpighian Tubules  (540.9) Larval Fat Body  (744.3) Larval Salivary Gland    302 Larval Trachea    377.55 Larval Carcass    320.4 Adult Head    227.9 Adult Eye    200.375 Adult Brain    236.3 Adult Thoracic-Abdominal Ganglion    185.5 Adult Crop    327.2 Adult Midgut    334.9 Adult Hindgut    261.1 Adult Malpighian Tubules    467 Adult Fat Body    388.7 Adult Salivary Gland    310.7 Adult Heart    406.15 Adult VirginFemale Spermatheca  (880.7) Adult InseminatedFemale Spermatheca  (700.2) Adult Ovary    308.5 Adult Testis    71.5 Adult Male Accessory Gland    335.4 Adult Carcass    342.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    218.575 Larval Midgut    438.1 Larval Hindgut    277.6 Larval Malpighian Tubules    540.9 Larval Fat Body    744.3 Larval Salivary Gland    302 Larval Trachea    377.55 Larval Carcass    320.4 Adult Head    227.9 Adult Eye    200.375 Adult Brain    236.3 Adult Thoracic-Abdominal Ganglion    185.5 Adult Crop    327.2 Adult Midgut    334.9 Adult Hindgut    261.1 Adult Malpighian Tubules    467 Adult Fat Body    388.7 Adult Salivary Gland    310.7 Adult Heart    406.15 Adult VirginFemale Spermatheca    880.7 Adult InseminatedFemale Spermatheca    700.2 Adult Ovary    308.5 Adult Testis    71.5 Adult Male Accessory Gland    335.4 Adult Carcass    342.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    218.575 Larval Midgut    438.1 Larval Hindgut    277.6 Larval Malpighian Tubules    540.9 Larval Fat Body    744.3 Larval Salivary Gland    302 Larval Trachea    377.55 Larval Carcass    320.4 Adult Head    227.9 Adult Eye    200.375 Adult Brain    236.3 Adult Thoracic-Abdominal Ganglion    185.5 Adult Crop    327.2 Adult Midgut    334.9 Adult Hindgut    261.1 Adult Malpighian Tubules    467 Adult Fat Body    388.7 Adult Salivary Gland    310.7 Adult Heart    406.15 Adult VirginFemale Spermatheca    880.7 Adult InseminatedFemale Spermatheca    700.2 Adult Ovary    308.5 Adult Testis    71.5 Adult Male Accessory Gland    335.4 Adult Carcass    342.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    218.575 Larval Midgut    438.1 Larval Hindgut    277.6 Larval Malpighian Tubules    540.9 Larval Fat Body    744.3 Larval Salivary Gland    302 Larval Trachea    377.55 Larval Carcass    320.4 Adult Head    227.9 Adult Eye    200.375 Adult Brain    236.3 Adult Thoracic-Abdominal Ganglion    185.5 Adult Crop    327.2 Adult Midgut    334.9 Adult Hindgut    261.1 Adult Malpighian Tubules    467 Adult Fat Body    388.7 Adult Salivary Gland    310.7 Adult Heart    406.15 Adult VirginFemale Spermatheca    880.7 Adult InseminatedFemale Spermatheca    700.2 Adult Ovary    308.5 Adult Testis    71.5 Adult Male Accessory Gland    335.4 Adult Carcass    342.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    218.575 Larval Midgut    438.1 Larval Hindgut    277.6 Larval Malpighian Tubules  540.9 Larval Fat Body  744.3 Larval Salivary Gland    302 Larval Trachea    377.55 Larval Carcass    320.4 Adult Head    227.9 Adult Eye    200.375 Adult Brain    236.3 Adult Thoracic-Abdominal Ganglion    185.5 Adult Crop    327.2 Adult Midgut    334.9 Adult Hindgut    261.1 Adult Malpighian Tubules    467 Adult Fat Body    388.7 Adult Salivary Gland    310.7 Adult Heart    406.15 Adult VirginFemale Spermatheca  (880.7) Adult InseminatedFemale Spermatheca  700.2 Adult Ovary    308.5 Adult Testis    71.5 Adult Male Accessory Gland    335.4 Adult Carcass    342.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    218.575 Larval Midgut    438.1 Larval Hindgut    277.6 Larval Malpighian Tubules    540.9 Larval Fat Body    744.3 Larval Salivary Gland    302 Larval Trachea    377.55 Larval Carcass    320.4 Adult Head    227.9 Adult Eye    200.375 Adult Brain    236.3 Adult Thoracic-Abdominal Ganglion    185.5 Adult Crop    327.2 Adult Midgut    334.9 Adult Hindgut    261.1 Adult Malpighian Tubules    467 Adult Fat Body    388.7 Adult Salivary Gland    310.7 Adult Heart    406.15 Adult VirginFemale Spermatheca    880.7 Adult InseminatedFemale Spermatheca    700.2 Adult Ovary    308.5 Adult Testis    71.5 Adult Male Accessory Gland    335.4 Adult Carcass    342.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    218.575 Larval Midgut    438.1 Larval Hindgut    277.6 Larval Malpighian Tubules    540.9 Larval Fat Body    744.3 Larval Salivary Gland    302 Larval Trachea    377.55 Larval Carcass    320.4 Adult Head    227.9 Adult Eye    200.375 Adult Brain    236.3 Adult Thoracic-Abdominal Ganglion    185.5 Adult Crop    327.2 Adult Midgut    334.9 Adult Hindgut    261.1 Adult Malpighian Tubules    467 Adult Fat Body    388.7 Adult Salivary Gland    310.7 Adult Heart    406.15 Adult VirginFemale Spermatheca    880.7 Adult InseminatedFemale Spermatheca    700.2 Adult Ovary    308.5 Adult Testis    71.5 Adult Male Accessory Gland    335.4 Adult Carcass    342.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    227.9   NA  Eye    200.375   NA  Brain    236.3   218.575  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    185.5   NA  Crop    327.2   438.1  Midgut    334.9   277.6  Hindgut    261.1   540.9  Malpighian Tubules    467   744.3  Fat Body    388.7   302  Salivary Gland    310.7   NA  Heart    406.15   377.55  Trachea    NA   NA  VirginFemale Spermatheca    880.7   NA  InseminatedFemale Spermatheca    700.2   NA  Ovary    308.5   NA  Testis    71.5   NA  Male Accessory Gland    335.4   320.4  Carcass    342.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    15 central nervous system, pupae P8    13 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    10 head, mated 20-day female    14 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    12 fat body, larvae L3 wandering    11 fat body, white prepupae    20 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    19 ovary, mated 4-day female    15 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    15 central nervous system, pupae P8    13 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    10 head, mated 20-day female    14 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae  (32) digestive system, larvae L3 wandering  (29) digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    12 fat body, larvae L3 wandering    11 fat body, white prepupae    20 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    19 ovary, mated 4-day female    15 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    15 central nervous system, pupae P8    13 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    10 head, mated 20-day female    14 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    12 fat body, larvae L3 wandering    11 fat body, white prepupae    20 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    19 ovary, mated 4-day female    15 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    15 central nervous system, pupae P8    13 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    10 head, mated 20-day female    14 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    12 fat body, larvae L3 wandering    11 fat body, white prepupae    20 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    19 ovary, mated 4-day female    15 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    15 central nervous system, pupae P8    13 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    10 head, mated 20-day female    14 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    12 fat body, larvae L3 wandering    11 fat body, white prepupae    20 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    19 ovary, mated 4-day female    15 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    15 central nervous system, pupae P8    13 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    10 head, mated 20-day female    14 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae  32 digestive system, larvae L3 wandering  29 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    12 fat body, larvae L3 wandering    11 fat body, white prepupae    20 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    19 ovary, mated 4-day female    15 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    15 central nervous system, pupae P8    13 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    10 head, mated 20-day female    14 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    12 fat body, larvae L3 wandering    11 fat body, white prepupae    20 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    19 ovary, mated 4-day female    15 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    15 central nervous system, pupae P8    13 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    10 head, mated 20-day female    14 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    12 fat body, larvae L3 wandering    11 fat body, white prepupae    20 fat body, pupae P8    4 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    19 ovary, mated 4-day female    15 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during late larval stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    95 embryo 04-06hr    60 embryo 06-08hr    33 embryo 08-10hr    31 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    38 embryo 16-18hr    50 embryo 18-20hr    46 embryo 20-22hr    44 embryo 22-24hr    41 larva L1    22 larva L2    16 larva L3 12hr old    17 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    51 white prepupae new    59 white prepupae 12hr    52 white prepupae 24hr    36 pupae 2d postWPP    26 pupae 3d postWPP    19 pupae 4d postWPP    21 adult male 01day    23 adult male 05day    23 adult male 30day    22 adult female 01day    32 adult female 05day    28 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (36) embryo 02-04hr  (95) embryo 04-06hr  (60) embryo 06-08hr  (33) embryo 08-10hr  (31) embryo 10-12hr  (28) embryo 12-14hr    23 embryo 14-16hr  (38) embryo 16-18hr  (50) embryo 18-20hr  (46) embryo 20-22hr  (44) embryo 22-24hr  (41) larva L1    22 larva L2    16 larva L3 12hr old    17 larva L3 puffstage 1-2  26 larva L3 puffstage 3-6  (40) larva L3 puffstage 7-9  (51) white prepupae new  (59) white prepupae 12hr  (52) white prepupae 24hr  (36) pupae 2d postWPP  26 pupae 3d postWPP    19 pupae 4d postWPP    21 adult male 01day    23 adult male 05day    23 adult male 30day    22 adult female 01day  (32) adult female 05day  (28) adult female 30day  (27) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    95 embryo 04-06hr    60 embryo 06-08hr    33 embryo 08-10hr    31 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    38 embryo 16-18hr    50 embryo 18-20hr    46 embryo 20-22hr    44 embryo 22-24hr    41 larva L1    22 larva L2    16 larva L3 12hr old    17 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    51 white prepupae new    59 white prepupae 12hr    52 white prepupae 24hr    36 pupae 2d postWPP    26 pupae 3d postWPP    19 pupae 4d postWPP    21 adult male 01day    23 adult male 05day    23 adult male 30day    22 adult female 01day    32 adult female 05day    28 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    95 embryo 04-06hr    60 embryo 06-08hr    33 embryo 08-10hr    31 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    38 embryo 16-18hr    50 embryo 18-20hr    46 embryo 20-22hr    44 embryo 22-24hr    41 larva L1    22 larva L2    16 larva L3 12hr old    17 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    51 white prepupae new    59 white prepupae 12hr    52 white prepupae 24hr    36 pupae 2d postWPP    26 pupae 3d postWPP    19 pupae 4d postWPP    21 adult male 01day    23 adult male 05day    23 adult male 30day    22 adult female 01day    32 adult female 05day    28 adult female 30day    27 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    95 embryo 04-06hr    60 embryo 06-08hr    33 embryo 08-10hr    31 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    38 embryo 16-18hr    50 embryo 18-20hr    46 embryo 20-22hr    44 embryo 22-24hr    41 larva L1    22 larva L2    16 larva L3 12hr old    17 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    51 white prepupae new    59 white prepupae 12hr    52 white prepupae 24hr    36 pupae 2d postWPP    26 pupae 3d postWPP    19 pupae 4d postWPP    21 adult male 01day    23 adult male 05day    23 adult male 30day    22 adult female 01day    32 adult female 05day    28 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (36) embryo 02-04hr  (95) embryo 04-06hr  (60) embryo 06-08hr  (33) embryo 08-10hr  31 embryo 10-12hr  28 embryo 12-14hr    23 embryo 14-16hr  (38) embryo 16-18hr  (50) embryo 18-20hr  (46) embryo 20-22hr  (44) embryo 22-24hr  (41) larva L1    22 larva L2    16 larva L3 12hr old    17 larva L3 puffstage 1-2  26 larva L3 puffstage 3-6  (40) larva L3 puffstage 7-9  (51) white prepupae new  (59) white prepupae 12hr  (52) white prepupae 24hr  (36) pupae 2d postWPP  26 pupae 3d postWPP    19 pupae 4d postWPP    21 adult male 01day    23 adult male 05day    23 adult male 30day    22 adult female 01day  32 adult female 05day  28 adult female 30day  27 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    95 embryo 04-06hr    60 embryo 06-08hr    33 embryo 08-10hr    31 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    38 embryo 16-18hr    50 embryo 18-20hr    46 embryo 20-22hr    44 embryo 22-24hr    41 larva L1    22 larva L2    16 larva L3 12hr old    17 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    51 white prepupae new    59 white prepupae 12hr    52 white prepupae 24hr    36 pupae 2d postWPP    26 pupae 3d postWPP    19 pupae 4d postWPP    21 adult male 01day    23 adult male 05day    23 adult male 30day    22 adult female 01day    32 adult female 05day    28 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    95 embryo 04-06hr    60 embryo 06-08hr    33 embryo 08-10hr    31 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    38 embryo 16-18hr    50 embryo 18-20hr    46 embryo 20-22hr    44 embryo 22-24hr    41 larva L1    22 larva L2    16 larva L3 12hr old    17 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    51 white prepupae new    59 white prepupae 12hr    52 white prepupae 24hr    36 pupae 2d postWPP    26 pupae 3d postWPP    19 pupae 4d postWPP    21 adult male 01day    23 adult male 05day    23 adult male 30day    22 adult female 01day    32 adult female 05day    28 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    20 embryonic 1182-4H    28 embryonic GM2    23 embryonic Kc167    27 embryonic S1    30 embryonic S3    21 leg disc CME L1    14 wing disc CME-W2    21 wing disc ML-DmD8    21 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    18 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    29 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    21 ovary fGS/OSS    27 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    20 embryonic 1182-4H  (28) embryonic GM2    23 embryonic Kc167  (27) embryonic S1  (30) embryonic S3    21 leg disc CME L1    14 wing disc CME-W2    21 wing disc ML-DmD8    21 wing disc ML-DmD9  (28) wing disc ML-DmD16-c3    12 wing disc ML-DmD21    18 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3  (29) eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    21 ovary fGS/OSS  (27) ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    20 embryonic 1182-4H    28 embryonic GM2    23 embryonic Kc167    27 embryonic S1    30 embryonic S3    21 leg disc CME L1    14 wing disc CME-W2    21 wing disc ML-DmD8    21 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    18 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    29 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    21 ovary fGS/OSS    27 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    20 embryonic 1182-4H    28 embryonic GM2    23 embryonic Kc167    27 embryonic S1    30 embryonic S3    21 leg disc CME L1    14 wing disc CME-W2    21 wing disc ML-DmD8    21 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    18 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    29 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    21 ovary fGS/OSS    27 ovary OSC    19 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    20 embryonic 1182-4H    28 embryonic GM2    23 embryonic Kc167    27 embryonic S1    30 embryonic S3    21 leg disc CME L1    14 wing disc CME-W2    21 wing disc ML-DmD8    21 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    18 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    29 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    21 ovary fGS/OSS    27 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    20 embryonic 1182-4H  28 embryonic GM2    23 embryonic Kc167  27 embryonic S1  30 embryonic S3    21 leg disc CME L1    14 wing disc CME-W2    21 wing disc ML-DmD8    21 wing disc ML-DmD9  28 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    18 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3  29 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    21 ovary fGS/OSS  27 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    20 embryonic 1182-4H    28 embryonic GM2    23 embryonic Kc167    27 embryonic S1    30 embryonic S3    21 leg disc CME L1    14 wing disc CME-W2    21 wing disc ML-DmD8    21 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    18 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    29 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    21 ovary fGS/OSS    27 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    20 embryonic 1182-4H    28 embryonic GM2    23 embryonic Kc167    27 embryonic S1    30 embryonic S3    21 leg disc CME L1    14 wing disc CME-W2    21 wing disc ML-DmD8    21 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    18 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    29 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    21 ovary fGS/OSS    27 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD38705 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035850 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7986-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Atg18 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | larval stage | recessive Atg18KG03090 viable, with Scer\GAL4pnr-MD237 Atg18GD12342 Other Phenotypes Allele decreased cell growth Atg18KG03090 Atg18Δ585 neuroanatomy defective Atg18KG03090 Atg18Δ585 stress response defective | larval stage | recessive Atg18KG03090 Phenotype manifest in Allele embryonic/larval fat body | somatic clone | third instar larval stage, with Scer\GAL4Act5C.PP Atg18JF02898 embryonic/larval fat body | third instar larval stage Atg18KG03090 embryonic/larval midgut | P-stage Atg18GD12342 embryonic/larval midgut | P-stage (with Df(3L)Exel6112) Atg18KG03090 embryonic/larval salivary gland (with Df(3L)66C-G28) Atg18KG03090 neuromuscular junction Atg18Δ585 Atg18KG03090 NMJ bouton Atg18Δ585 Atg18KG03090
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of Atg18 Class Mutagen Stocks Known lesion Atg185-SZ-3505 1 -- Atg18KG03090 P-element activity 1 -- Atg18G3744 P-element activity 0 -- Atg18Δ585 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Atg18 Class Mutagen Stocks Known lesion Atg18GD12342 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Atg18HMS01193 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Atg18JF02898 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Atg18KK100064 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Atg18tub.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Exel6112 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12342} NA P{KK100064} NA P{TRiP.HMS01193} NA P{TRiP.JF02898} NA characterization construct P{tub-EGFP-Atg18} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{RS5}Atg185-SZ-3505 NA P{SUPor-P}Atg18KG03090 NA insertion of mobile activating element P{EP}Atg18G3744 NA
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
autophagy inferred from mutant phenotype (Scott et al., 2004) larval midgut histolysis inferred from mutant phenotype (Denton et al., 2009) macroautophagy inferred from mutant phenotype (Scott et al., 2004) regulation of autophagy inferred from mutant phenotype (Pircs et al., 2012) regulation of defense response to virus inferred from mutant phenotype (Shelly et al., 2009)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
phosphatidylinositol-3,5-bisphosphate binding inferred from biological aspect of ancestor with PANTHER:PTN000132136 (assigned by RefGenome) (Gaudet et al., 2010) phosphatidylinositol-3-phosphate binding inferred from biological aspect of ancestor with PANTHER:PTN000132136 (assigned by RefGenome) (Gaudet et al., 2010)
Biological Process
CV term Evidence References
autophagic vacuole assembly inferred from biological aspect of ancestor with PANTHER:PTN000132136 (assigned by RefGenome) (Gaudet et al., 2010)
Cellular Component
CV term Evidence References
autophagic vacuole membrane inferred from biological aspect of ancestor with PANTHER:PTN000132137 (assigned by RefGenome) (Gaudet et al., 2010) cytoplasm inferred from biological aspect of ancestor with PANTHER:PTN000132136 (assigned by RefGenome) (Gaudet et al., 2010) cytosol inferred from biological aspect of ancestor with PANTHER:PTN000132137 (assigned by RefGenome) (Gaudet et al., 2010) pre-autophagosomal structure membrane inferred from biological aspect of ancestor with PANTHER:PTN000132137 (assigned by RefGenome) (Gaudet et al., 2010)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Atg18 - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - CG10641 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - CG14786 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - CG43154 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - Elongin-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - Hsp22 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - Hsp23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - Hsp27 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - Hsp68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - Hsp70Aa anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - Pgam5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - Rpb8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - SmD2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - mRpL39 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - mRpL43 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - mRpL44 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - mRpS28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - mRpS33 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - p38b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg18 - ref(2)P anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Atg18 allele Gene References unspecified bchs (Simonsen et al., 2007)
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0035850 DroID - A comprehensive database of gene and protein interactions. • FBgn0035850 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38913 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VSF0
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6H1BFP Dipteran inclusive ortholog search EOG669R3B Insect inclusive ortholog search EOG65HQTB Arthropod inclusive ortholog search EOG6DR7V8 Metazoa inclusive ortholog search EOG6P32VN
Orthologs in Drosophila Species (EOG6H1BFP)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Atg18     Drosophila simulans   Dsim\GD14059 Y   Drosophila sechellia   Dsec\GM25025 Y   Drosophila erecta   Dere\GG14476 Y   Drosophila yakuba   Dyak\GE21663 Y   Drosophila ananassae   Dana\GF25099 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20742 Y   Drosophila persimilis   Dper\GL22534 Y   Drosophila willistoni   Dwil\GK11999 Y   Drosophila virilis   Dvir\GJ12513 Y   Drosophila mojavensis   Dmoj\GI16769 Y   Drosophila grimshawi   Dgri\GH15493 Y
Orthologs in non-Drosophila Dipterans (EOG669R3B)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013995   Anopheles gambiae Malaria mosquito  Agam\AGAP005910
Orthologs in non-Dipteran Insects (EOG65HQTB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49949 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG008528 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15724 Y Atta cephalotes Leafcutter ant  Acep\ACEP22413 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO15536 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17671 Y Linepithema humile Argentine ant  Lhum\LH22825 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB23519 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI006216 Y Pediculus humanus Human body louse  Phum\PHUM084890 Y Tribolium castaneum Red flour beetle  Tcas\TC006217 Y
Orthologs in non-Insect Arthropods (EOG6DR7V8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\CCDC37 Y Ixodes scapularis Deer tick  Isca\ISCW018244 Y
Orthologs in non-Arthropod Metazoa (EOG6P32VN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\atg-18 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006318gn Y Danio rerio Zebrafish  Drer\ENSDARG00000037357 Y Danio rerio Zebrafish  Drer\wipi1 Y Xenopus tropicalis Western clawed frog  Xtro\wipi1 Y Gallus gallus Domestic chicken  Ggal\WIPI1 Y Gallus gallus Domestic chicken  Ggal\WIPI2 Y Mus musculus House mouse  Mmus\Wipi2 Y Mus musculus House mouse  Mmus\Wipi1 Y Rattus norvegicus Norway rat  Rnor\Wipi1 Y Rattus norvegicus Norway rat  Rnor\Wipi2 Y Homo sapiens Human  Hsap\WIPI2 Y Homo sapiens Human  Hsap\WIPI1 Y
Human Orthologs (2)
Gene OMIM HGNC Hsap\WIPI2     Hsap\WIPI1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD14059 Drosophila sechellia Dsec\GM25025 Drosophila erecta Dere\GG14476 Drosophila yakuba Dyak\GE21663 Drosophila ananassae Dana\GF25099 Drosophila pseudoobscura pseudoobscura Dpse\GA20742 Drosophila persimilis Dper\GL22534 Drosophila willistoni Dwil\GK11999 Drosophila virilis Dvir\GJ12513 Drosophila mojavensis Dmoj\GI16769 Drosophila grimshawi Dgri\GH15493
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	28061 y1 v1; P{TRiP.JF02898}attP2 34714 y1 sc* v1; P{TRiP.HMS01193}attP2 13945 y1; P{SUPor-P}Atg18KG03090 ry506/TM3, Sb1 Ser1
Kyoto	126105 w1118; P{RS5}Atg185-SZ-3505
VDRC	v105366 P{KK100064}VIE-260B v22643 w1118; P{GD12342}v22643 v22646 w1118; P{GD12342}v22646/TM3
Genomic Clones ( 2 )
	BACR06E04 BACR09I19 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 60 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD38705
Other clones	DPiM_LD38705
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH10353 GM06583 LD04617 LD12054 LD13202 LD16938 LD18534 LD32514 LD37009 LD45423 LD45781 LD47019 LP03279 LP05928 LP06196 LP06857 LP14280 LP17877 RE11488 RE14505 RE21789 RE44096 RE57168 SD17439
Other clones	34573 37060 38931 42100 61924 79858 82380 107552 148612 192956 214309.82 281005 CK01.65A.D7 CK01.130C.H10 CK01.143B.E3 EK053016 EK054232 EK116318 EK145009 EK147701 EK147825 EK156124 EK172543 EK185345 EK188506 EK211926 EK213349 EK233156 EK244531 EK254328 EK295825 EK305910 RE01931 UUGC1107
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0035850 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38913
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Atg18 CG7986 (Scott, 2005.3.2) Source for identity of: Atg18 CG7986 (Scott et al., 2004)
Source for database merge of
Additional comments
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. (Pilot et al., 2006)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_139927 NM_168259 NM_206301 NM_001274635 NM_001274636 NM_001274637 RefSeq (Proteins) • NP_648184 NP_729341 NP_996023 NP_001261564 NP_001261565 NP_001261566 DDBJ / EMBL / GenBank • AAF50471 AAF50472 AAK93323 AAS65056 AGB94259 AGB94260 AGB94261 AI519194 AW943438 AY051899 BH809427 Entrez Gene - A searchable database of RefSeq genes. • 38913 UniProtKB/TrEMBL • Q9VSF0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • WD40-repeat-containing domain (IPR017986) WD40 repeat (IPR001680) WD40/YVTN repeat-like-containing domain (IPR015943) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD38705
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0035850 DroID - A comprehensive database of gene and protein interactions. • FBgn0035850 DRSC - Results from RNAi screens. • FBgn0035850 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035850 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7986-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0035850 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38913 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38913 modMine - Data generated by the modENCODE project. • FBgn0035850 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VSF0
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	ATG18 (Scott, 2005.3.2) Atg18 (Zeitouni et al., 2007, Jungreis, 2011.11.18, Tian et al., 2011, Rusten et al., 2006, Shelly et al., 2009, Liu and Lehmann, 2008, Denton et al., 2009, Curtis et al., 2007, Köhler et al., 2009, Simonsen et al., 2008, Érdi et al., 2012, Kim et al., 2010) atg18 (Berry and Baehrecke, 2007, Simonsen et al., 2007, Milton et al., 2011, Wang et al., 2008, Shen and Ganetzky, 2009) Atg18a (Pircs et al., 2012) CG7986 (Pilot et al., 2006, Quinones-Coello, 2007, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2010.12.1, Érdi et al., 2012)
Name Synonym	Autophagy-specific gene 18 (Scott et al., 2004, Scott, 2005.3.2, Scott et al., 2004)
Secondary FlyBase IDs
References ( 42 )
Generate a list of	All references relating to this gene ( 42 )
List References by type	Research paper  ( 21 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 10 ) FlyBase analysis  ( 2 ) Website  ( 2 ) Curated genome annotation  ( 1 )
Recent research papers ( 6 )
	Velentzas et al., 2013, Cell Biol. Toxicol. 29(1): 13--37 Detrimental effects of proteasome inhibition activity in Drosophila melanogaster: implication of ER stress, autophagy, and apoptosis. [FBrf0220377] Denton et al., 2012, Cell Death Differ. 19(8): 1299--1307 Relationship between growth arrest and autophagy in midgut programmed cell death in Drosophila. [FBrf0218812] Pircs et al., 2012, PLoS ONE 7(8): e44214 Advantages and Limitations of Different p62-Based Assays for Estimating Autophagic Activity in Drosophila. [FBrf0219364] Érdi et al., 2012, Autophagy 8(7): 1124--1135 Loss of the starvation-induced gene Rack1 leads to glycogen deficiency and impaired autophagic responses in Drosophila. [FBrf0219844] Milton et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(42): 17521--17526 Oxidative stress induces overgrowth of the Drosophila neuromuscular junction. [FBrf0216440] Tian et al., 2011, Nat. Neurosci. 14(10): 1267--1275 Drosophila Rae1 controls the abundance of the ubiquitin ligase Highwire in post-mitotic neurons. [FBrf0216253] Show all research papers ...