FlyBase Gene Report: Dmel\Zasp66

General Information

Symbol

Dmel\Zasp66

Species

D. melanogaster

Name

Z band alternatively spliced PDZ-motif protein 66

Annotation Symbol

CG6416

Feature Type

protein_coding_gene

FlyBase ID

FBgn0035917

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Z band alternatively spliced PDZ-motif protein 66 (Zasp66) encodes a scaffold protein that binds the product of Actn and localizes to Z-discs in muscle cells. It plays a role in muscle development, especially in myofibril assembly. [Date last reviewed: 2018-10-18] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

66D9-66D9

Sequence location

3L:8,624,371..8,638,813 [+]

Recombination map

3-26

RefSeq locus

NT_037436 REGION:8624371..8638813

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables muscle alpha-actinin binding

inferred from physical interaction with FLYBASE:Actn; FB:FBgn0000667

(Katzemich et al., 2013)

enables protein phosphatase 1 binding

inferred from physical interaction with FLYBASE:flw; FB:FBgn0000711

(Bennett et al., 2006)

inferred from physical interaction with FLYBASE:Pp1-13C; FB:FBgn0003132

(Bennett et al., 2006)

inferred from physical interaction with FLYBASE:Pp1α-96A; FB:FBgn0003134

(Bennett et al., 2006)

inferred from physical interaction with FLYBASE:Pp1-87B; FB:FBgn0004103

(Bennett et al., 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables actin binding

inferred from biological aspect of ancestor with PANTHER:PTN001198141

(GO Reference Genome Project, 2011-)

enables muscle alpha-actinin binding

inferred from biological aspect of ancestor with PANTHER:PTN001198141

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in mesoderm development

inferred from expression pattern

(Furlong et al., 2001)

involved_in myofibril assembly

inferred from mutant phenotype

(Katzemich et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in actin cytoskeleton organization

inferred from biological aspect of ancestor with PANTHER:PTN001198141

(GO Reference Genome Project, 2011-)

involved_in muscle structure development

inferred from biological aspect of ancestor with PANTHER:PTN001198141

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in Z disc

inferred from direct assay

(Katzemich et al., 2013, Hudson et al., 2008)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in adherens junction

inferred from biological aspect of ancestor with PANTHER:PTN001198141

(GO Reference Genome Project, 2011-)

part_of filamentous actin

inferred from biological aspect of ancestor with PANTHER:PTN001198141

(GO Reference Genome Project, 2011-)

is_active_in stress fiber

inferred from biological aspect of ancestor with PANTHER:PTN001198141

(GO Reference Genome Project, 2011-)

is_active_in Z disc

inferred from biological aspect of ancestor with PANTHER:PTN001198150

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Zasp66 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8IQB4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Alternative translation stop created by use of multiphasic reading frames within coding region.

Variable use of small exons; supported combination results in frameshift in downstream exons.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Zasp66-RA

FBtr0089562

NM_168286

1212

239

Zasp66-RB

FBtr0089568

NM_139989

1482

298

Zasp66-RE

FBtr0089561

NM_168283

1725

430

Zasp66-RF

FBtr0089563

NM_168284

1714

402

Zasp66-RG

FBtr0089564

NM_176305

786

165

Zasp66-RH

FBtr0089565

NM_176304

1120

135

Zasp66-RI

FBtr0089566

NM_176303

1280

215

Zasp66-RJ

FBtr0089567

NM_176306

1352

239

Zasp66-RK

FBtr0301154

NM_001169911

1642

378

Zasp66-RM

FBtr0301156

NM_001169913

1554

322

Zasp66-RN

FBtr0301157

NM_001169914

1793

406

Zasp66-RO

FBtr0301158

NM_001169915

1493

326

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Zasp66-PA

FBpp0088530

27.2

239

9.48

FBpp0089007

33.0

298

8.07

FBpp0088529

47.6

430

9.79

FBpp0088531

44.5

402

8.95

FBpp0088532

19.4

165

9.19

FBpp0088533

15.7

135

9.42

FBpp0088534

24.5

215

9.21

FBpp0088535

27.2

239

9.48

FBpp0290377

41.8

378

8.04

FBpp0290379

35.7

322

9.14

FBpp0290380

44.9

406

9.65

FBpp0290381

36.1

326

9.89

E1JI74

NP_001163386

ACZ94657

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

239 aa isoforms: Zasp66-PA, Zasp66-PJ

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Zasp66 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.56

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

indirect flight muscle cell

• Z disc

(González-Morales et al., 2019)

mesothoracic tergotrochanter muscle cell

• Z disc

(González-Morales et al., 2019)

adult midgut muscle cell

• Z disc

(González-Morales et al., 2019)

muscle cell of leg muscle of leg

• Z disc

(González-Morales et al., 2019)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Zasp66 localizes to Z discs of indirect flight muscle but also to Z discs of the other muscles analyzed (jump muscle, leg muscle, and midgut muscle).

(González-Morales et al., 2019)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in Z disc

inferred from direct assay

(Katzemich et al., 2013, Hudson et al., 2008)

Expression Deduced from Reporters

Reporter: P{PTT-GA}Zasp66^ZCL0663

Stage

Tissue/Position (including subcellular localization)

Reference

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Zasp66 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Zasp66

View exonic expression by tissue for Dmel\Zasp66

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035917

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38988/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035917

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035917

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035917

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6416

Flygut - An atlas of the Drosophila adult midgut

FBgn0035917

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Zasp66

Transgenic constructs containing regulatory region of Zasp66

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 1 )

Dp(3;2)GV-CH321-17N24

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Zasp66^CC00858

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

Zasp66^KK112973

partially lethal, with Scer\GAL4^Mef2.PR

Zasp66^KK112973

partially lethal - majority die

Zasp66^CRISPR

viable

Zasp66^ZCL0663

Sterility

Allele

fertile

Zasp66^ZCL0663

Other Phenotypes

Allele

flightless, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

Zasp66^KK112973

majority die during P-stage

Zasp66^CRISPR

majority die during pupal stage, with Scer\GAL4^Mef2.PR

Zasp66^KK112973

Phenotype manifest in

Allele

indirect flight muscle cell

Zasp66^CRISPR

indirect flight muscle cell, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

Zasp66^KK112973

indirect flight muscle cell, with Scer\GAL4^Act88F.PB

Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

M band, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

Zasp66^KK112973

myofibril, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

Zasp66^KK112973

Z disc, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

Zasp66^KK112973

Z disc, with Scer\GAL4^Mef2.PR

Zasp66^KK112973

Z disc | adult stage

Zasp66^CRISPR

Z disc | adult stage, with Scer\GAL4^Act88F.PB

Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (32)

Hsap\LDB3

2 of 14

Yes

Hsap\PDLIM1

2 of 14

Yes

Hsap\PDLIM2

2 of 14

Yes

Hsap\PDLIM3

2 of 14

Yes

Hsap\PDLIM4

2 of 14

Yes

Hsap\PDLIM5

2 of 14

Yes

Hsap\PDLIM7

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (30)

Rnor\Ldb3

2 of 14

Yes

Rnor\Pdlim1

2 of 14

Yes

Rnor\Pdlim2

2 of 14

Yes

Rnor\Pdlim3

2 of 14

Yes

Rnor\Pdlim4

2 of 14

Yes

Rnor\Pdlim5

2 of 14

Yes

Rnor\Pdlim7

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Tamalin

1 of 14

Rnor\Ush1c

1 of 14

Rnor\Whrn

1 of 14

Mus musculus (laboratory mouse) (32)

Mmus\Ldb3

2 of 14

Yes

Mmus\Pdlim1

2 of 14

Yes

Mmus\Pdlim2

2 of 14

Yes

Mmus\Pdlim3

2 of 14

Yes

Mmus\Pdlim4

2 of 14

Yes

Mmus\Pdlim5

2 of 14

Yes

Mmus\Pdlim7

2 of 14

Yes

Mmus\Cytip

1 of 14

Mmus\Dlg5

1 of 14

Mmus\Gm20498

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Tamalin

1 of 14

Mmus\Ush1c

1 of 14

Mmus\Whrn

1 of 14

Xenopus tropicalis (Western clawed frog) (14)

Xtro\pdlim1

2 of 13

Yes

Xtro\pdlim5

2 of 13

Yes

Xtro\pdlim7

2 of 13

Yes

Xtro\adcyap1r1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\pdlim4

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (29)

Drer\ldb3a

2 of 14

Yes

Drer\pdlim1

2 of 14

Yes

Drer\pdlim2

2 of 14

Yes

Drer\pdlim3a

2 of 14

Yes

Drer\pdlim3b

2 of 14

Yes

Drer\pdlim4

2 of 14

Yes

Drer\pdlim5a

2 of 14

Yes

Drer\pdlim5b

2 of 14

Yes

Drer\pdlim7

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\tamalin

1 of 14

Drer\ush1c

1 of 14

Drer\whrna

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (17)

Cele\alp-1

1 of 14

Yes

Cele\C46H11.6

1 of 14

Yes

Cele\C50F7.3

1 of 14

Yes

Cele\C52A11.3

1 of 14

Yes

Cele\F16G10.5

1 of 14

Yes

Cele\F20D6.1

1 of 14

Yes

Cele\F28E10.4

1 of 14

Yes

Cele\F40F9.3

1 of 14

Yes

Cele\gras-1

1 of 14

Yes

Cele\mics-1

1 of 14

Yes

Cele\mpz-4

1 of 14

Yes

Cele\smz-1

1 of 14

Yes

Cele\smz-2

1 of 14

Yes

Cele\T19B10.5

1 of 14

Yes

Cele\txt-1

1 of 14

Yes

Cele\ZK849.1

1 of 14

Yes

Cele\ZK1321.4

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (23)

Agam\AgaP_AGAP006260

9 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP012008

1 of 12

Agam\AgaP_AGAP012354

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Zasp66. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (26)

CG42319

2 of 13

Zasp67

2 of 13

1 of 13

baz

1 of 13

bbg

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

ameliorates myofibrillar myopathy 4

modeled by Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Zasp66 - Actn

anti tag coimmunoprecipitation, western blot

(Katzemich et al., 2013)

Zasp66 - Pp1-13C

two hybrid

(Bennett et al., 2006)

Zasp66 - Pp1-87B

two hybrid

(Bennett et al., 2006)

Zasp66 - Pp1α-96A

two hybrid

(Bennett et al., 2006)

Zasp66 - Zasp52

two hybrid, anti tag coimmunoprecipitation, western blot, bimolecular fluorescence complementation, fluorescence microscopy

(González-Morales et al., 2019, Katzemich et al., 2013)

Zasp66 - flw

two hybrid

(Bennett et al., 2006)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Zasp66

enhanceable

Zasp52

(Katzemich et al., 2013)

Zasp66

enhanceable

Zasp67

(González-Morales et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Zasp52

suppressible

Zasp66

(González-Morales et al., 2019)

Zasp52

enhanceable

Zasp66

(Katzemich et al., 2013)

Zasp67

enhanceable

Zasp66

(González-Morales et al., 2019)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64396

DroID - A comprehensive database of gene and protein interactions.

FBgn0035917

MIST (protein-protein) - An integrated Molecular Interaction Database

38988

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

E1JI74

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-26

Cytogenetic map

66D9-66D9

Sequence location

3L:8,624,371..8,638,813 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66D9-66D9

Limits computationally determined from genome sequence between P{lacW}foi^j8E8 and P{PZ}SrpRβ^rK561&P{lacW}l(3)j5B6^j5B6

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-26

(FlyBase, 2016)

3-20.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

6824

y¹ w¹¹¹⁸; P{PTT-GA}Zasp66^ZCL0663

93472

w^*; TI{CRIMIC.TG4.0}Zasp66^{CR60134-TG4.0}/TM3, Sb¹

64050

w¹¹¹⁸; PBac{IT.GAL4}Cdc6^0908-G4 Zasp66^0908-G4/TM6B, Tb¹

Kyoto

110740

y^* w^* P{PTT-GA}Zasp66^ZCL0663

104039

y^* w^*; P{GawB}Zasp66^NP1586 / TM6, P{UAS-lacZ.UW23-1}UW23-1

6416R-2

v330136

v102980

v342800

P{hsFLP}1, y1 w1118; P{HD_CFD01959}attP40/CyO-GFP

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (202)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0035917

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0035917

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG6416 anon-WO0153538.30

(FlyBase, 1996-)

Additional comments

Source for merge of CG6416 anon-WO0153538.30 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Zasp66 CG6416

(Hudson et al., 2008)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG6416

(Meadows, 2017.1.11, Çiçek et al., 2016, Rosa-Ferreira et al., 2015, Ashwal-Fluss et al., 2014, Wang et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Cammarato et al., 2011, Langer et al., 2010, Schnorrer et al., 2010, Keleman et al., 2009.8.5, Lebo et al., 2009, Hudson et al., 2008, Buszczak et al., 2007, Quinones-Coello, 2007, Quinones-Coello et al., 2007, Bennett et al., 2006, Montana and Littleton, 2006)

ZASP66

(Irion, 2012)

Zaps66

(Bryantsev and Cripps, 2012)

anon-WO0153538.30

Name Synonyms

CG6416

Z band alternatively spliced PDZ-motif protein 66

Secondary FlyBase IDs

FBgn0045924

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 86 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38988

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8IQB4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8IQB4

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035917

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38988/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035917

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035917

FlyMine - An integrated database for Drosophila genomics

FBgn0035917

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6416

MARRVEL_MODEL - MARRVEL (model organism gene)

38988

Linkouts

BioGRID - A database of protein and genetic interactions.

64396

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0035917

DroID - A comprehensive database of gene and protein interactions.

FBgn0035917

DRSC - Results frm RNAi screens

FBgn0035917

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035917

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0035917

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6416

Flygut - An atlas of the Drosophila adult midgut

FBgn0035917

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014041

MIST (protein-protein) - An integrated Molecular Interaction Database

38988

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

E1JI74

References (68)

The following external sites may use different assemblies or annotations than FlyBase.

-0.56

Report Sections