dTrpA1, dANKTM1, DmTRPA1, Transient receptor potential A1, TrpA
heat-activated TRP family cation channel that is essential for thermotaxis - controls thermotaxis at innocuous temperatures, as well as thermal and chemical nociception in response to noxious heat and chemical exposure
Initial two exons previously annotated as final exons of one alternative transcript of CG5747 mfr. Species conservation data supporst the current gene structures.
Gene model reviewed during 5.45
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
Gene model reviewed during 6.32
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\TrpA1 using the Feature Mapper tool.
TrpA1 immunoreactivity was observed across most of the anterior midgut and in two lateral domains across middle midgut boundaries facing the anterior or posterior midgut. TrpA1-expressing cells were closely associated with immunoreactivity of the enteroendocrine cell nuclear marker, pros, but not with those of intestinal stem cell and enteroblast markers.
TrpA1 protein expression was observed in the mouthparts in neurons innervating sensilla numbers 8 and 9 of the labral sense organ.
GBrowse - Visual display of RNA-Seq signalsView Dmel\TrpA1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: TrpA1 Anktm1
TrpA1 functions in temperature control of circadian rhythm in pacemaker neurons.
The isoform referred to as TRPA1(B) in FBrf0222493 is called "TRPA1-A" in FBrf0217493. The isoform referred to as TRPA1(A) in FBrf0222493 is similar to that called "TRPA1-D" in FBrf0217493, except that "TRPA1(A)" has an additional N-terminal amino acids.
The TRPA1(B) isoform encodes a non-selective cation channel that can be activated by heat, voltage and chemicals.
The "TRPA1(B)" isoform is activated by noxious chemicals when expressed in Xenopus oocytes and expression of this isoform can restore an avoidance response to noxious chemicals in TrpA1 mutant flies. However, the expression of this isoform inside the head should minimise its exposure to environmental irritants, and thus in wild-type flies its primary function may be as a warmth sensor.
The "TRPA1(A)" isoform is much less thermosensitive than TRPA1(B) when expressed in Xenopus oocytes and does not confer thermal sensitivity on neurons when expressed in them. Expression of this isoform can restore an avoidance response to noxious chemicals in TrpA1 mutant flies.
S2R+ cells expressing the "TRP1-A" isoform show heat-induced Ca[2+] responses.
S2R+ cells expressing the "TRPA1-B" isoform lack Ca[2+] responses to temperature in the 20-42[o]C range, but show Ca[2+] responses to an irritant chemical.
The "TRPA1-C" isoform rescues thermal nociception phenotypes when expressed in TrpA1 mutant nociceptors. S2R+ cells expressing this isoform do not show a meaningful response to temperatures in the range of 15-42[o]C, which suggests that "TRPA1-C" is not acting directly as a noxious heat sensor. S2R+ cells expressing this isoform do show Ca[2+] responses to an irritant chemical.
S2R+ cells expressing the "TRPA1-D" isoform show Ca[2+] responses significantly above the baseline beginning at a temperature of 34[o]C.
TrpA1 is required cell autonomously for light transduction in class IV dendritic arborization neurons.
Larvae injected with dsRNA against TrpA1 show abnormal thermotactic behaviour, while their chemotactic response to n-octyl acetate and response to being touched with a hot (55oC) probe is normal.
The threshold for response of the "TRPA1(B)" isoform is 24-29[o]C.