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General Information
Symbol
Dmel\TrpA1
Species
D. melanogaster
Name
Transient receptor potential cation channel A1
Annotation Symbol
CG5751
Feature Type
FlyBase ID
FBgn0035934
Gene Model Status
Stock Availability
Gene Snapshot
Transient receptor potential cation channel A1 (TrpA1) encodes a cation channel activated by warming and by reactive chemicals. Its roles include the control of thermotaxis at innocuous temperatures, as well as thermal and chemical nociception in response to noxious heat and chemical exposure. [Date last reviewed: 2019-03-14]
Also Known As

dTrpA1, dANKTM1, DmTRPA1, Transient receptor potential A1, TrpA

Key Links
Genomic Location
Cytogenetic map
Sequence location
3L:8,894,404..8,905,200 [-]
Recombination map

3-27

RefSeq locus
NT_037436 REGION:8894404..8905200
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the transient receptor (TC 1.A.4) family. (Q7Z020)
Summaries
Gene Group (FlyBase)
TRANSIENT RECEPTOR POTENTIAL CHANNELS -
The Transient Receptor Potential (TRP) channel family are cation channels conserved from invertebrates to humans. TRP channels respond to diverse stimuli and are involved in a number of environmental sensory pathways. (Adapted from FBrf0220930 and FBrf0225477).
Protein Function (UniProtKB)
Essential for thermotaxis by sensing environmental temperature. Receptor-activated non-selective cation channel involved in detection of sensations such as temperature. Involved in heat nociception by being activated by warm temperature of about 24-29 degrees Celsius.
(UniProt, Q7Z020)
Summary (Interactive Fly)

heat-activated TRP family cation channel that is essential for thermotaxis - controls thermotaxis at innocuous temperatures, as well as thermal and chemical nociception in response to noxious heat and chemical exposure

Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
5

Please see the GBrowse view of Dmel\TrpA1 or the JBrowse view of Dmel\TrpA1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Initial two exons previously annotated as final exons of one alternative transcript of CG5747 mfr. Species conservation data supporst the current gene structures.

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Gene model reviewed during 6.32

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0331823
4573
1232
FBtr0331824
4570
1231
FBtr0331825
4259
1197
FBtr0331826
4256
1196
FBtr0331827
4462
1195
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0304207
138.8
1232
7.67
FBpp0304208
139.1
1231
7.92
FBpp0304209
135.2
1197
7.84
FBpp0304210
135.4
1196
8.08
FBpp0304211
134.7
1195
7.68
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Homotetramer.

(UniProt, Q7Z020)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\TrpA1 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (25 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (0 terms)
Biological Process (18 terms)
Terms Based on Experimental Evidence (17 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity
inferred from electronic annotation with InterPro:IPR005821
(assigned by InterPro )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred by curator from GO:0005261
(assigned by UniProt )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

TrpA1 immunoreactivity was observed across most of the anterior midgut and in two lateral domains across middle midgut boundaries facing the anterior or posterior midgut. TrpA1-expressing cells were closely associated with immunoreactivity of the enteroendocrine cell nuclear marker, pros, but not with those of intestinal stem cell and enteroblast markers.

TrpA1 protein expression was observed in the mouthparts in neurons innervating sensilla numbers 8 and 9 of the labral sense organ.

TrpA1 is expressed in two uncharacterised groups of cells in the adult brain: the lateral cells (LC) and ventral cells (VC).

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
Reporter: P{TrpA1-GAL4.C-D}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{TrpA1-GAL4.R}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{TrpA1-GAL4.SH}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{TrpA1-QF.P}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: PBac{TrpA1-GAL4.P}
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: TI{GAL4}TrpA1-CD
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\TrpA1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 13 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 41 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of TrpA1
Transgenic constructs containing regulatory region of TrpA1
Deletions and Duplications ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (10)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
1 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (11)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (6)
10 of 13
Yes
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (4)
8 of 12
Yes
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (9)
10 of 15
Yes
Yes
6 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
11 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (1)
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
1 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
1 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900TV )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915003Y )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00VX )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00UG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01BG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (10)
3 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Homotetramer.
    (UniProt, Q7Z020 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map

    3-27

    Cytogenetic map
    Sequence location
    3L:8,894,404..8,905,200 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    66E3-66E3
    Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (31)
    Genomic Clones (25)
    cDNA Clones (0)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
            Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              Antibody Information
              Laboratory Generated Antibodies
               
              Commercially Available Antibodies
               
              Other Information
              Relationship to Other Genes
              Source for database identify of

              Source for identity of: TrpA1 Anktm1

              Source for database merge of
              Additional comments
              Other Comments

              TrpA1 functions in temperature control of circadian rhythm in pacemaker neurons.

              The isoform referred to as TRPA1(B) in FBrf0222493 is called "TRPA1-A" in FBrf0217493. The isoform referred to as TRPA1(A) in FBrf0222493 is similar to that called "TRPA1-D" in FBrf0217493, except that "TRPA1(A)" has an additional N-terminal amino acids.

              The TRPA1(B) isoform encodes a non-selective cation channel that can be activated by heat, voltage and chemicals.

              The "TRPA1(B)" isoform is activated by noxious chemicals when expressed in Xenopus oocytes and expression of this isoform can restore an avoidance response to noxious chemicals in TrpA1 mutant flies. However, the expression of this isoform inside the head should minimise its exposure to environmental irritants, and thus in wild-type flies its primary function may be as a warmth sensor.

              The "TRPA1(A)" isoform is much less thermosensitive than TRPA1(B) when expressed in Xenopus oocytes and does not confer thermal sensitivity on neurons when expressed in them. Expression of this isoform can restore an avoidance response to noxious chemicals in TrpA1 mutant flies.

              S2R+ cells expressing the "TRP1-A" isoform show heat-induced Ca[2+] responses.

              S2R+ cells expressing the "TRPA1-B" isoform lack Ca[2+] responses to temperature in the 20-42[o]C range, but show Ca[2+] responses to an irritant chemical.

              The "TRPA1-C" isoform rescues thermal nociception phenotypes when expressed in TrpA1 mutant nociceptors. S2R+ cells expressing this isoform do not show a meaningful response to temperatures in the range of 15-42[o]C, which suggests that "TRPA1-C" is not acting directly as a noxious heat sensor. S2R+ cells expressing this isoform do show Ca[2+] responses to an irritant chemical.

              S2R+ cells expressing the "TRPA1-D" isoform show Ca[2+] responses significantly above the baseline beginning at a temperature of 34[o]C.

              TrpA1 is required for thermal nociception in both larvae and adult flies.

              TrpA1 is essential for class IV dendritic arborization neuron light responses.

              TrpA1 is required cell autonomously for light transduction in class IV dendritic arborization neurons.

              TrpA1 functions as a molecular sensor of warmth.

              TrpA1 is required for normal thermotactic behaviour.

              Larvae injected with dsRNA against TrpA1 show abnormal thermotactic behaviour, while their chemotactic response to n-octyl acetate and response to being touched with a hot (55oC) probe is normal.

              The threshold for response of the "TRPA1(B)" isoform is 24-29[o]C.

              Origin and Etymology
              Discoverer
              Etymology
              Identification
              External Crossreferences and Linkouts ( 40 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              Flygut - An atlas of the Drosophila adult midgut
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              KEGG Genes - Molecular building blocks of life in the genomic space.
              modMine - A data warehouse for the modENCODE project
              SignaLink - A signaling pathway resource with multi-layered regulatory networks.
              Linkouts
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              FLIGHT - Cell culture data for RNAi and other high-throughput technologies
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              FlyMine - An integrated database for Drosophila genomics
              Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
              MIST (genetic) - An integrated Molecular Interaction Database
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              Synonyms and Secondary IDs (28)
              Reported As
              Symbol Synonym
              Anktm1
              TrpA1
              (FlyBase Genome Annotators, 2019-, Ni et al., 2019, Xu et al., 2019, Zhang et al., 2019, Aboudhiaf et al., 2018, Jang et al., 2018, Kim et al., 2018, Ly et al., 2018, Scheunemann et al., 2018, Shimell et al., 2018, Yadlapalli et al., 2018, Zhao et al., 2018, Allada et al., 2017, Artiushin and Sehgal, 2017, Aw et al., 2017, Chen et al., 2017, Machado et al., 2017, Sitaraman et al., 2017, Tang et al., 2017, Clandinin and Owens, 2016-, Clark et al., 2016, Du et al., 2016, Gene Disruption Project members, 2016-, Sokabe et al., 2016, Soukup et al., 2016, Burn et al., 2015, Gene Disruption Project members, 2015-, Green et al., 2015, Head et al., 2015, Seidner et al., 2015, Bjordal et al., 2014, Boto et al., 2014, Doll and Broadie, 2014, Honda et al., 2014, Lin et al., 2014, Liu et al., 2014, Menuz et al., 2014, Rezával et al., 2014, Sun et al., 2014, Calcagno et al., 2013, Devineni et al., 2013, Flood et al., 2013, Flood et al., 2013, Gillespie and Hodge, 2013, Ni et al., 2013, Short and Lazzaro, 2013, Sivachenko et al., 2013, Vonhoff et al., 2013, Yamanaka et al., 2013, Zhang et al., 2013, Zhu, 2013, Bolukbasi et al., 2012, Kang et al., 2012, Kaun et al., 2012, Keleman et al., 2012, Lu et al., 2012, Majumdar et al., 2012, Plaçais et al., 2012, Rezaval et al., 2012, Talsma et al., 2012, Thistle et al., 2012, Tsubouchi et al., 2012, Yoshihara and Ito, 2012, Donlea et al., 2011, Kadowaki, 2011.10.7, Kadowaki, 2011.10.7, Neely et al., 2011, Shang et al., 2011, von Philipsborn et al., 2011, Alekseyenko et al., 2010, Kang et al., 2010, Kong et al., 2010, Xiang et al., 2010, Garrity, 2008.12.9, Kwon et al., 2008)
              Name Synonyms
              Transient receptor potential A 1
              Transient receptor potential cation channel
              Transient receptor potential cation channel A1
              Transient receptor potential cation channel A1 ortholog
              transient receptor potential A1
              transient-receptor-potential A1
              Secondary FlyBase IDs
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                References (385)