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Gene Dmel\Use1

General Information
Symbol	Dmel\Use1	Species	D. melanogaster
Name	Use1	Annotation symbol	CG14181
Feature type	protein_coding_gene	FlyBase ID	FBgn0035965
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	67A1-67A1	Sequence location	3L:9,128,809..9,130,281 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Use1 is referred to in FlyBase by the symbol Dmel\Use1 (CG14181, FBgn0035965). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: Golgi organization; protein secretion; neurogenesis. 5 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Vesicle transport protein, Use1. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult salivary gland, adult ovary, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1624014_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Use1. Gene sequence location is 3L:9128809..9130281.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms day 42 of adulthood day 41 of adulthood day 30 of adulthood day 49 of adulthood day 17 of adulthood day 45 of adulthood day 8 of adulthood day 26 of adulthood day 21 of adulthood day 13 of adulthood day 25 of adulthood day 20 of adulthood day 22 of adulthood day 39 of adulthood day 7 of adulthood day 36 of adulthood day 11 of adulthood day 37 of adulthood day 27 of adulthood day 18 of adulthood day 19 of adulthood day 28 of adulthood day 9 of adulthood day 29 of adulthood adult heart day 40 of adulthood day 16 of adulthood day 24 of adulthood day 15 of adulthood day 33 of adulthood day 43 of adulthood day 47 of adulthood day 38 of adulthood day 32 of adulthood day 48 of adulthood day 23 of adulthood day 35 of adulthood day 12 of adulthood day 10 of adulthood day 31 of adulthood day 34 of adulthood day 44 of adulthood day 14 of adulthood day 46 of adulthood
FB2012_01	Sequence features BKN23046 HFA10180
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 67A1-67A1   Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Use1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.40
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Use1-RA FBtr0076537  NM_140032   980   250
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Use1-PA   FBpp0076264   28.3   250   10.02     NP_648289   AAF50314
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Vesicle transport protein, Use1 (IPR019150)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF50314   BF496897   BT011417   CO177932   CO344749   CZ467166   CZ467815   EC069748   EC252625
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VSU7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0035965    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0035965 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0035965 expression level Developmental Stage   Expression Level embryo 00-02hr    1823 embryo 02-04hr    869 embryo 04-06hr    985 embryo 06-08hr    955 embryo 08-10hr    859 embryo 10-12hr    622 embryo 12-14hr    725 embryo 14-16hr    277 embryo 16-18hr    380 embryo 18-20hr    365 embryo 20-22hr    406 embryo 22-24hr    469 larva L1    299 larva L2    691 larva L3 12hr old    517 larva L3 puffstage 1-2    566 larva L3 puffstage 3-6    723 larva L3 puffstage 7-9    932 white prepupae new    847 white prepupae 12hr    748 white prepupae 24hr    762 pupae 2d postWPP    675 pupae 3d postWPP    492 pupae 4d postWPP    225 adult male 01day    527 adult male 05day    610 adult male 30day    402 adult female 01day    366 adult female 05day    1526 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1823) embryo 02-04hr  (869) embryo 04-06hr  (985) embryo 06-08hr  (955) embryo 08-10hr  (859) embryo 10-12hr  (622) embryo 12-14hr  (725) embryo 14-16hr  (277) embryo 16-18hr  (380) embryo 18-20hr  (365) embryo 20-22hr  (406) embryo 22-24hr  (469) larva L1  (299) larva L2  (691) larva L3 12hr old  (517) larva L3 puffstage 1-2  (566) larva L3 puffstage 3-6  (723) larva L3 puffstage 7-9  (932) white prepupae new  (847) white prepupae 12hr  (748) white prepupae 24hr  (762) pupae 2d postWPP  (675) pupae 3d postWPP  (492) pupae 4d postWPP  (225) adult male 01day  (527) adult male 05day  (610) adult male 30day  (402) adult female 01day  (366) adult female 05day  (1526) adult female 30day  (1547) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (1823) embryo 02-04hr    869 embryo 04-06hr    985 embryo 06-08hr    955 embryo 08-10hr    859 embryo 10-12hr    622 embryo 12-14hr    725 embryo 14-16hr    277 embryo 16-18hr    380 embryo 18-20hr    365 embryo 20-22hr    406 embryo 22-24hr    469 larva L1    299 larva L2    691 larva L3 12hr old    517 larva L3 puffstage 1-2    566 larva L3 puffstage 3-6    723 larva L3 puffstage 7-9    932 white prepupae new    847 white prepupae 12hr    748 white prepupae 24hr    762 pupae 2d postWPP    675 pupae 3d postWPP    492 pupae 4d postWPP    225 adult male 01day    527 adult male 05day    610 adult male 30day    402 adult female 01day    366 adult female 05day  (1526) adult female 30day  (1547) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1823 embryo 02-04hr    869 embryo 04-06hr    985 embryo 06-08hr    955 embryo 08-10hr    859 embryo 10-12hr    622 embryo 12-14hr    725 embryo 14-16hr    277 embryo 16-18hr    380 embryo 18-20hr    365 embryo 20-22hr    406 embryo 22-24hr    469 larva L1    299 larva L2    691 larva L3 12hr old    517 larva L3 puffstage 1-2    566 larva L3 puffstage 3-6    723 larva L3 puffstage 7-9    932 white prepupae new    847 white prepupae 12hr    748 white prepupae 24hr    762 pupae 2d postWPP    675 pupae 3d postWPP    492 pupae 4d postWPP    225 adult male 01day    527 adult male 05day    610 adult male 30day    402 adult female 01day    366 adult female 05day    1526 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1823 embryo 02-04hr    869 embryo 04-06hr    985 embryo 06-08hr    955 embryo 08-10hr    859 embryo 10-12hr    622 embryo 12-14hr    725 embryo 14-16hr    277 embryo 16-18hr    380 embryo 18-20hr    365 embryo 20-22hr    406 embryo 22-24hr    469 larva L1    299 larva L2    691 larva L3 12hr old    517 larva L3 puffstage 1-2    566 larva L3 puffstage 3-6    723 larva L3 puffstage 7-9    932 white prepupae new    847 white prepupae 12hr    748 white prepupae 24hr    762 pupae 2d postWPP    675 pupae 3d postWPP    492 pupae 4d postWPP    225 adult male 01day    527 adult male 05day    610 adult male 30day    402 adult female 01day    366 adult female 05day    1526 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0035965 expression level Developmental Stage   Expression Level embryo 00-02hr    1823 embryo 02-04hr    869 embryo 04-06hr    985 embryo 06-08hr    955 embryo 08-10hr    859 embryo 10-12hr    622 embryo 12-14hr    725 embryo 14-16hr    277 embryo 16-18hr    380 embryo 18-20hr    365 embryo 20-22hr    406 embryo 22-24hr    469 larva L1    299 larva L2    691 larva L3 12hr old    517 larva L3 puffstage 1-2    566 larva L3 puffstage 3-6    723 larva L3 puffstage 7-9    932 white prepupae new    847 white prepupae 12hr    748 white prepupae 24hr    762 pupae 2d postWPP    675 pupae 3d postWPP    492 pupae 4d postWPP    225 adult male 01day    527 adult male 05day    610 adult male 30day    402 adult female 01day    366 adult female 05day    1526 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1823) embryo 02-04hr  (869) embryo 04-06hr  (985) embryo 06-08hr  (955) embryo 08-10hr  (859) embryo 10-12hr  (622) embryo 12-14hr  (725) embryo 14-16hr  (277) embryo 16-18hr  (380) embryo 18-20hr  (365) embryo 20-22hr  (406) embryo 22-24hr  (469) larva L1  (299) larva L2  (691) larva L3 12hr old  (517) larva L3 puffstage 1-2  (566) larva L3 puffstage 3-6  (723) larva L3 puffstage 7-9  (932) white prepupae new  (847) white prepupae 12hr  (748) white prepupae 24hr  (762) pupae 2d postWPP  (675) pupae 3d postWPP  (492) pupae 4d postWPP  (225) adult male 01day  (527) adult male 05day  (610) adult male 30day  (402) adult female 01day  (366) adult female 05day  (1526) adult female 30day  (1547) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  1823 embryo 02-04hr    869 embryo 04-06hr    985 embryo 06-08hr    955 embryo 08-10hr    859 embryo 10-12hr    622 embryo 12-14hr    725 embryo 14-16hr    277 embryo 16-18hr    380 embryo 18-20hr    365 embryo 20-22hr    406 embryo 22-24hr    469 larva L1    299 larva L2    691 larva L3 12hr old    517 larva L3 puffstage 1-2    566 larva L3 puffstage 3-6    723 larva L3 puffstage 7-9    932 white prepupae new    847 white prepupae 12hr    748 white prepupae 24hr    762 pupae 2d postWPP    675 pupae 3d postWPP    492 pupae 4d postWPP    225 adult male 01day    527 adult male 05day    610 adult male 30day    402 adult female 01day    366 adult female 05day  1526 adult female 30day  1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1823 embryo 02-04hr    869 embryo 04-06hr    985 embryo 06-08hr    955 embryo 08-10hr    859 embryo 10-12hr    622 embryo 12-14hr    725 embryo 14-16hr    277 embryo 16-18hr    380 embryo 18-20hr    365 embryo 20-22hr    406 embryo 22-24hr    469 larva L1    299 larva L2    691 larva L3 12hr old    517 larva L3 puffstage 1-2    566 larva L3 puffstage 3-6    723 larva L3 puffstage 7-9    932 white prepupae new    847 white prepupae 12hr    748 white prepupae 24hr    762 pupae 2d postWPP    675 pupae 3d postWPP    492 pupae 4d postWPP    225 adult male 01day    527 adult male 05day    610 adult male 30day    402 adult female 01day    366 adult female 05day    1526 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1823 embryo 02-04hr    869 embryo 04-06hr    985 embryo 06-08hr    955 embryo 08-10hr    859 embryo 10-12hr    622 embryo 12-14hr    725 embryo 14-16hr    277 embryo 16-18hr    380 embryo 18-20hr    365 embryo 20-22hr    406 embryo 22-24hr    469 larva L1    299 larva L2    691 larva L3 12hr old    517 larva L3 puffstage 1-2    566 larva L3 puffstage 3-6    723 larva L3 puffstage 7-9    932 white prepupae new    847 white prepupae 12hr    748 white prepupae 24hr    762 pupae 2d postWPP    675 pupae 3d postWPP    492 pupae 4d postWPP    225 adult male 01day    527 adult male 05day    610 adult male 30day    402 adult female 01day    366 adult female 05day    1526 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0035965    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0035965 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult salivary gland, adult ovary, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0035965 expression level Tissue   Expression Level Larval Central Nervous System    70.6 Larval Midgut    54.1 Larval Hindgut    60.6 Larval Malpighian Tubules    69.9 Larval Fat Body    63.4 Larval Salivary Gland    167.3 Larval Trachea    61.925 Larval Carcass    48.475 Adult Head    46.7 Adult Eye    62.575 Adult Brain    49.6 Adult Thoracic-Abdominal Ganglion    41.6 Adult Crop    71.9 Adult Midgut    50.3 Adult Hindgut    50.6 Adult Malpighian Tubules    41.2 Adult Fat Body no informative data Adult Salivary Gland    170.5 Adult Heart    35.95 Adult VirginFemale Spermatheca    29.8 Adult InseminatedFemale Spermatheca    43.9 Adult Ovary    112 Adult Testis    60.7 Adult Male Accessory Gland    159.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    70.6 Larval Midgut    54.1 Larval Hindgut    60.6 Larval Malpighian Tubules    69.9 Larval Fat Body    63.4 Larval Salivary Gland    167.3 Larval Trachea    61.925 Larval Carcass    48.475 Adult Head    46.7 Adult Eye    62.575 Adult Brain    49.6 Adult Thoracic-Abdominal Ganglion    41.6 Adult Crop    71.9 Adult Midgut    50.3 Adult Hindgut    50.6 Adult Malpighian Tubules    41.2 Adult Fat Body no informative data Adult Salivary Gland    170.5 Adult Heart    35.95 Adult VirginFemale Spermatheca    29.8 Adult InseminatedFemale Spermatheca    43.9 Adult Ovary    112 Adult Testis    60.7 Adult Male Accessory Gland    159.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    70.6 Larval Midgut    54.1 Larval Hindgut    60.6 Larval Malpighian Tubules    69.9 Larval Fat Body    63.4 Larval Salivary Gland    167.3 Larval Trachea    61.925 Larval Carcass    48.475 Adult Head    46.7 Adult Eye    62.575 Adult Brain    49.6 Adult Thoracic-Abdominal Ganglion    41.6 Adult Crop    71.9 Adult Midgut    50.3 Adult Hindgut    50.6 Adult Malpighian Tubules    41.2 Adult Fat Body no informative data Adult Salivary Gland    170.5 Adult Heart    35.95 Adult VirginFemale Spermatheca    29.8 Adult InseminatedFemale Spermatheca    43.9 Adult Ovary    112 Adult Testis    60.7 Adult Male Accessory Gland    159.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    70.6 Larval Midgut    54.1 Larval Hindgut    60.6 Larval Malpighian Tubules    69.9 Larval Fat Body    63.4 Larval Salivary Gland    167.3 Larval Trachea    61.925 Larval Carcass    48.475 Adult Head    46.7 Adult Eye    62.575 Adult Brain    49.6 Adult Thoracic-Abdominal Ganglion    41.6 Adult Crop    71.9 Adult Midgut    50.3 Adult Hindgut    50.6 Adult Malpighian Tubules    41.2 Adult Fat Body no informative data Adult Salivary Gland    170.5 Adult Heart    35.95 Adult VirginFemale Spermatheca    29.8 Adult InseminatedFemale Spermatheca    43.9 Adult Ovary    112 Adult Testis    60.7 Adult Male Accessory Gland    159.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0035965 expression level Tissue   Expression Level Larval Central Nervous System    70.6 Larval Midgut    54.1 Larval Hindgut    60.6 Larval Malpighian Tubules    69.9 Larval Fat Body    63.4 Larval Salivary Gland    167.3 Larval Trachea    61.925 Larval Carcass    48.475 Adult Head    46.7 Adult Eye    62.575 Adult Brain    49.6 Adult Thoracic-Abdominal Ganglion    41.6 Adult Crop    71.9 Adult Midgut    50.3 Adult Hindgut    50.6 Adult Malpighian Tubules    41.2 Adult Fat Body no informative data Adult Salivary Gland    170.5 Adult Heart    35.95 Adult VirginFemale Spermatheca    29.8 Adult InseminatedFemale Spermatheca    43.9 Adult Ovary    112 Adult Testis    60.7 Adult Male Accessory Gland    159.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    70.6 Larval Midgut    54.1 Larval Hindgut    60.6 Larval Malpighian Tubules    69.9 Larval Fat Body    63.4 Larval Salivary Gland    167.3 Larval Trachea    61.925 Larval Carcass    48.475 Adult Head    46.7 Adult Eye    62.575 Adult Brain    49.6 Adult Thoracic-Abdominal Ganglion    41.6 Adult Crop    71.9 Adult Midgut    50.3 Adult Hindgut    50.6 Adult Malpighian Tubules    41.2 Adult Fat Body no informative data Adult Salivary Gland    170.5 Adult Heart    35.95 Adult VirginFemale Spermatheca    29.8 Adult InseminatedFemale Spermatheca    43.9 Adult Ovary    112 Adult Testis    60.7 Adult Male Accessory Gland    159.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    70.6 Larval Midgut    54.1 Larval Hindgut    60.6 Larval Malpighian Tubules    69.9 Larval Fat Body    63.4 Larval Salivary Gland    167.3 Larval Trachea    61.925 Larval Carcass    48.475 Adult Head    46.7 Adult Eye    62.575 Adult Brain    49.6 Adult Thoracic-Abdominal Ganglion    41.6 Adult Crop    71.9 Adult Midgut    50.3 Adult Hindgut    50.6 Adult Malpighian Tubules    41.2 Adult Fat Body no informative data Adult Salivary Gland    170.5 Adult Heart    35.95 Adult VirginFemale Spermatheca    29.8 Adult InseminatedFemale Spermatheca    43.9 Adult Ovary    112 Adult Testis    60.7 Adult Male Accessory Gland    159.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    70.6 Larval Midgut    54.1 Larval Hindgut    60.6 Larval Malpighian Tubules    69.9 Larval Fat Body    63.4 Larval Salivary Gland    167.3 Larval Trachea    61.925 Larval Carcass    48.475 Adult Head    46.7 Adult Eye    62.575 Adult Brain    49.6 Adult Thoracic-Abdominal Ganglion    41.6 Adult Crop    71.9 Adult Midgut    50.3 Adult Hindgut    50.6 Adult Malpighian Tubules    41.2 Adult Fat Body no informative data Adult Salivary Gland    170.5 Adult Heart    35.95 Adult VirginFemale Spermatheca    29.8 Adult InseminatedFemale Spermatheca    43.9 Adult Ovary    112 Adult Testis    60.7 Adult Male Accessory Gland    159.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    46.7   NA  Eye    62.575   NA  Brain    49.6   70.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    41.6   NA  Crop    71.9   54.1  Midgut    50.3   60.6  Hindgut    50.6   69.9  Malpighian Tubules    41.2   63.4  Fat Body    no informative data   167.3  Salivary Gland    170.5   NA  Heart    35.95   61.925  Trachea    NA   NA  VirginFemale Spermatheca    29.8   NA  InseminatedFemale Spermatheca    43.9   NA  Ovary    112   NA  Testis    60.7   NA  Male Accessory Gland    159.3   48.475  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_23
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035965 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14181-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 Use1GD2382 lethal, with Scer\GAL4pnr-MD237 Use1GD2382 lethal | pupal stage, with Scer\GAL4pnr-MD237 Use1GD2382 Other Phenotypes Allele neuroanatomy defective | larval stage, with Scer\GAL41407 Use1GD2382 Phenotype manifest in Allele ganglion mother cell | larval stage, with Scer\GAL41407 Use1GD2382
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of Use1 Class Mutagen Stocks Known lesion Use1c02266 piggyBac transposase 1 -- Use1c01432 piggyBac transposase 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Use1 Class Mutagen Stocks Known lesion Use1GD2382 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Use1KK102950 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Use1T:SV5\V5 in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD2382} NA P{KK102950} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}Use1c01432 NA PBac{PB}Use1c02266 NA insertion of mobile activating element P{EPgy2}EY01228 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
Golgi organization inferred from mutant phenotype (Bard et al., 2006) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) protein secretion inferred from mutant phenotype (Bard et al., 2006)
Cellular Component
CV term Evidence References
endoplasmic reticulum inferred from direct assay (Bard et al., 2006)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Use1 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0035965 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39051
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23584 Dere\GG15036 Dgri\GH15917 Dmoj\GI16569 Dper\GL15075 Dpse\GA12809 Dsec\GM24890 Dsim\GD12941 Dvir\GJ12821 Dwil\GK24344 Dyak\GE21260
Curated drosophilid orthologs	Dpse\GA12809
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL007532-PA Aedes aegypti (mosquito) AGAP005893-PA Anopheles gambiae (mosquito) XP_001946677.1 Acyrthosiphon pisum (pea aphid) 125999 Branchiostoma floridae (lancelet) XP_001894875.1 Brugia malayi (roundworm nematode) BGIBMGA001916-PA Bombyx mori (silk worm) ENSBTAP00000014785 Bos taurus (bovine) CN19535 Caenorhabditis brenneri (small nematode) CE34394 Caenorhabditis elegans (worm) ENSCAFP00000022832 Canis familiaris (dog) ENSCINP00000015282 Ciona intestinalis (sea squirt) JA14973 Caenorhabditis japonica (nematode) CPIJ000097 Culex pipens (mosquito) ENSCPOP00000018452 Cavia porcellus (guinea pig) RP05920 Caenorhabditis remanei (nematode) ENSCSAVP00000017014 Ciona savignyi (sedentary tunicate) 205502 Capitella spl (polychaete worm) 102626 Daphnia pulex (daphnia) ENSDARP00000024706 Danio rerio (zebra fish) ENSECAP00000016138 Equus caballus (horse) ENSGACP00000013865 Gymnopilus aculeatus (mushroom) ENSGALP00000034378 Gallus gallus (chicken) 185094 Hypsipyla robusta (cedar shoot borer) ENSP00000263897 Homo sapiens (human) ISCW021367-PA Ixodes scapularis (deer tick) 102889 Lottia gigantea (owl limpet) ENSMODP00000018805 Monodelphis domestica (gray opossum) ENSMMUP00000015224 Macaca mulatta (rhesus monkey) ENSMUSP00000105681 Mus musculus (house mouse) 116334 Nematostella vectensis (starlet anemone) ENSORLP00000024286 Oryzias latipes (japanese killifish) PHUM008461-PA Pediculus humanus (human body louse) PP18047 Pristionchus pacificus (nematode) ENSPPYP00000010872 Pongo pygmaeus (orangutan) ENSPTRP00000018192 Pan troglodytes (chimpanzee) ENSRNOP00000022650 Rattus norvegicus (norway rat) SPAC17G6.07c Schizosaccharomyces pombe (fission yeast) XP_787597.1 Strongylocentrotus purpuratus (purple sea urchin) 54646 Trichoplax adhaerens (metazoa) XP_973973.1 Tribolium castaneum (red flour beetle) ENSTNIP00000018588 Tetraodon nigroviridis (green spotted pufferfish) ENSXETP00000029601 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • LH18477 ISCW021367 FBgn0238529 FBgn0226304 FBgn0200056 FBgn0184665 FBgn0179752 JGI_V11_102626 FBgn0072854 FBgn0152679 FBgn0139317 FBgn0123388 FBgn0107290 FBgn0100578 CPIJ000097 BGIBMGA001916 ACYPI009171 GB16719 AGAP005893 AAEL007532 PHUM308750 GLEAN_09646
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Harvard	- PBac{PB}Use1c02266
VDRC	v100019 P{KK102950}VIE-260B v42549 w1118; P{GD2382}v42549 v42548 w1118; P{GD2382}v42548/CyO
Genomic Clones ( 2 )
	BACR01E12 BACR03E24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 5 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT10903
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	214225.66 CK01.68B.G7 EK054728 EP19830
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0035965 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39051
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Use1 CG14181 (Bard et al., 2006)
Source for database merge of
Additional comments
Other Comments
	dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion. (Bard et al., 2006) dsRNA directed against Use1 inhibits protein secretion in S2 cells and results in fusion of Golgi membranes with the endoplasmic reticulum in more than 50% of the treated cells. (Bard et al., 2006)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140032 RefSeq (Proteins) • NP_648289 DDBJ / EMBL / GenBank • AAF50314 BF496897 CZ467166 CZ467815 Entrez Gene - A searchable database of RefSeq genes. • 39051 UniProtKB/TrEMBL • Q9VSU7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Vesicle transport protein, Use1 (IPR019150)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0035965 DRSC - Results from RNAi screens. • FBgn0035965 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035965 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14181-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0035965 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39051 InParanoid A subset of ortholog calls from InParanoid. • AAEL007532-PA Aedes aegypti (mosquito) AGAP005893-PA Anopheles gambiae (mosquito) XP_001946677.1 Acyrthosiphon pisum (pea aphid) 125999 Branchiostoma floridae (lancelet) XP_001894875.1 Brugia malayi (roundworm nematode) BGIBMGA001916-PA Bombyx mori (silk worm) ENSBTAP00000014785 Bos taurus (bovine) CN19535 Caenorhabditis brenneri (small nematode) CE34394 Caenorhabditis elegans (worm) ENSCAFP00000022832 Canis familiaris (dog) ENSCINP00000015282 Ciona intestinalis (sea squirt) JA14973 Caenorhabditis japonica (nematode) CPIJ000097 Culex pipens (mosquito) ENSCPOP00000018452 Cavia porcellus (guinea pig) RP05920 Caenorhabditis remanei (nematode) ENSCSAVP00000017014 Ciona savignyi (sedentary tunicate) 205502 Capitella spl (polychaete worm) 102626 Daphnia pulex (daphnia) ENSDARP00000024706 Danio rerio (zebra fish) ENSECAP00000016138 Equus caballus (horse) ENSGACP00000013865 Gymnopilus aculeatus (mushroom) ENSGALP00000034378 Gallus gallus (chicken) 185094 Hypsipyla robusta (cedar shoot borer) ENSP00000263897 Homo sapiens (human) ISCW021367-PA Ixodes scapularis (deer tick) 102889 Lottia gigantea (owl limpet) ENSMODP00000018805 Monodelphis domestica (gray opossum) ENSMMUP00000015224 Macaca mulatta (rhesus monkey) ENSMUSP00000105681 Mus musculus (house mouse) 116334 Nematostella vectensis (starlet anemone) ENSORLP00000024286 Oryzias latipes (japanese killifish) PHUM008461-PA Pediculus humanus (human body louse) PP18047 Pristionchus pacificus (nematode) ENSPPYP00000010872 Pongo pygmaeus (orangutan) ENSPTRP00000018192 Pan troglodytes (chimpanzee) ENSRNOP00000022650 Rattus norvegicus (norway rat) SPAC17G6.07c Schizosaccharomyces pombe (fission yeast) XP_787597.1 Strongylocentrotus purpuratus (purple sea urchin) 54646 Trichoplax adhaerens (metazoa) XP_973973.1 Tribolium castaneum (red flour beetle) ENSTNIP00000018588 Tetraodon nigroviridis (green spotted pufferfish) ENSXETP00000029601 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39051 modMine - Data generated by the modENCODE project. • FBgn0035965 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • LH18477 ISCW021367 FBgn0238529 FBgn0226304 FBgn0200056 FBgn0184665 FBgn0179752 JGI_V11_102626 FBgn0072854 FBgn0152679 FBgn0139317 FBgn0123388 FBgn0107290 FBgn0100578 CPIJ000097 BGIBMGA001916 ACYPI009171 GB16719 AGAP005893 AAEL007532 PHUM308750 GLEAN_09646
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG14181 (Bard et al., 2006, Munier et al., 2004, Keleman et al., 2009.8.5, Cammarato et al., 2011) FBgn 35965 (Lehmann et al., 2010) Use1
Name Synonym	Use1 (Cammarato et al., 2011)
Secondary FlyBase IDs
References ( 12 )
Research paper	Lehmann et al., 2010, BMC Evol. Biol. 10: 156 Some novel intron positions in conserved Drosophila genes are caused by intron sliding or tandem duplication. [FBrf0211127] Bard et al., 2006, Nature 439(7076): 604--607 Functional genomics reveals genes involved in protein secretion and Golgi organization. [FBrf0191232] Munier et al., 2004, Insect Biochem. Molec. Biol. 34(10): 1025--1035 graal: a Drosophila gene coding for several mosaic serine proteases. [FBrf0180344]
Supplementary material	Cammarato et al., 2011, PLoS ONE 6(4): Table S1. Proteins & Peptides. [FBrf0214682] Neumüller et al., 2011, Cell Stem Cell 8(5): Supplemental Table S1. [FBrf0214431] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]