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Gene Dmel\phol

General Information
Symbol	Dmel\phol	Species	D. melanogaster
Name	pleiohomeotic like	Annotation symbol	CG3445
Feature type	protein_coding_gene	FlyBase ID	FBgn0035997
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	67B7-67B8	Sequence location	3L:9,446,148..9,449,381 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene pleiohomeotic like is referred to in FlyBase by the symbol Dmel\phol (CG3445, FBgn0035997). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific DNA binding. There is experimental evidence that it is involved in the biological process: gene silencing. 5 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Zinc finger C2H2-type/integrase DNA-binding domain; Zinc finger, C2H2; Zinc finger, C2H2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1630080_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of phol. Gene sequence location is 3L:9446148..9449381.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 67B7-67B8   Limits computationally determined from genome sequence between P{EP}Hsp26EP3336&P{EP}Hsp26EP3315 and P{PZ}fry02240
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\phol for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) phol-RA FBtr0076475  NM_140060   3139   669
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein phol-PA   FBpp0076203   73.2   669   4.64     NP_648317   AAF50265
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger C2H2-type/integrase DNA-binding domain (IPR013087) Zinc finger, C2H2-like (IPR015880) Zinc finger, C2H2 (IPR007087)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA801524   AA950886   AE014296 AAF50265   AI064052   AI238881   AI456490   AI515855   AI516282   AQ025112   AW943062   AY119607 AAM50261   BF485854   BF497855   BG635490   BI212562   BI485150   BI591379   BI608130   BZ665776   CO277625   CO283350   CO314177   CO321804   CO329875   EC067370   EC199522   EC209186   EC214148   EC223276   EV594946   GH749552   GH752472   GH760961   GH801391   GH814973   GH863696   GH883920   GH898448
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VSZ3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Klymenko et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    183.8 Larval Midgut    49.8 Larval Hindgut    58.2 Larval Malpighian Tubules    97.4 Larval Fat Body    93.3 Larval Salivary Gland    151.1 Larval Trachea    228.225 Larval Carcass    66.775 Adult Head    66.8 Adult Eye    127.475 Adult Brain    146.5 Adult Thoracic-Abdominal Ganglion    98.7 Adult Crop    94.3 Adult Midgut    77.9 Adult Hindgut    64.4 Adult Malpighian Tubules    98.5 Adult Fat Body    60.9 Adult Salivary Gland    100.8 Adult Heart    93 Adult VirginFemale Spermatheca    96.3 Adult InseminatedFemale Spermatheca    91 Adult Ovary    334.3 Adult Testis    33.3 Adult Male Accessory Gland    135.2 Adult Carcass    64.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    183.8 Larval Midgut    49.8 Larval Hindgut    58.2 Larval Malpighian Tubules    97.4 Larval Fat Body    93.3 Larval Salivary Gland    151.1 Larval Trachea    228.225 Larval Carcass    66.775 Adult Head    66.8 Adult Eye    127.475 Adult Brain    146.5 Adult Thoracic-Abdominal Ganglion    98.7 Adult Crop    94.3 Adult Midgut    77.9 Adult Hindgut    64.4 Adult Malpighian Tubules    98.5 Adult Fat Body    60.9 Adult Salivary Gland    100.8 Adult Heart    93 Adult VirginFemale Spermatheca    96.3 Adult InseminatedFemale Spermatheca    91 Adult Ovary    334.3 Adult Testis    33.3 Adult Male Accessory Gland    135.2 Adult Carcass    64.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    183.8 Larval Midgut    49.8 Larval Hindgut    58.2 Larval Malpighian Tubules    97.4 Larval Fat Body    93.3 Larval Salivary Gland    151.1 Larval Trachea    228.225 Larval Carcass    66.775 Adult Head    66.8 Adult Eye    127.475 Adult Brain    146.5 Adult Thoracic-Abdominal Ganglion    98.7 Adult Crop    94.3 Adult Midgut    77.9 Adult Hindgut    64.4 Adult Malpighian Tubules    98.5 Adult Fat Body    60.9 Adult Salivary Gland    100.8 Adult Heart    93 Adult VirginFemale Spermatheca    96.3 Adult InseminatedFemale Spermatheca    91 Adult Ovary    334.3 Adult Testis    33.3 Adult Male Accessory Gland    135.2 Adult Carcass    64.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    183.8 Larval Midgut    49.8 Larval Hindgut    58.2 Larval Malpighian Tubules    97.4 Larval Fat Body    93.3 Larval Salivary Gland    151.1 Larval Trachea    228.225 Larval Carcass    66.775 Adult Head    66.8 Adult Eye    127.475 Adult Brain    146.5 Adult Thoracic-Abdominal Ganglion    98.7 Adult Crop    94.3 Adult Midgut    77.9 Adult Hindgut    64.4 Adult Malpighian Tubules    98.5 Adult Fat Body    60.9 Adult Salivary Gland    100.8 Adult Heart    93 Adult VirginFemale Spermatheca    96.3 Adult InseminatedFemale Spermatheca    91 Adult Ovary    334.3 Adult Testis    33.3 Adult Male Accessory Gland    135.2 Adult Carcass    64.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    183.8 Larval Midgut    49.8 Larval Hindgut    58.2 Larval Malpighian Tubules    97.4 Larval Fat Body    93.3 Larval Salivary Gland    151.1 Larval Trachea    228.225 Larval Carcass    66.775 Adult Head    66.8 Adult Eye    127.475 Adult Brain    146.5 Adult Thoracic-Abdominal Ganglion    98.7 Adult Crop    94.3 Adult Midgut    77.9 Adult Hindgut    64.4 Adult Malpighian Tubules    98.5 Adult Fat Body    60.9 Adult Salivary Gland    100.8 Adult Heart    93 Adult VirginFemale Spermatheca    96.3 Adult InseminatedFemale Spermatheca    91 Adult Ovary    334.3 Adult Testis    33.3 Adult Male Accessory Gland    135.2 Adult Carcass    64.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    183.8 Larval Midgut    49.8 Larval Hindgut    58.2 Larval Malpighian Tubules    97.4 Larval Fat Body    93.3 Larval Salivary Gland    151.1 Larval Trachea    228.225 Larval Carcass    66.775 Adult Head    66.8 Adult Eye    127.475 Adult Brain    146.5 Adult Thoracic-Abdominal Ganglion    98.7 Adult Crop    94.3 Adult Midgut    77.9 Adult Hindgut    64.4 Adult Malpighian Tubules    98.5 Adult Fat Body    60.9 Adult Salivary Gland    100.8 Adult Heart    93 Adult VirginFemale Spermatheca    96.3 Adult InseminatedFemale Spermatheca    91 Adult Ovary    334.3 Adult Testis    33.3 Adult Male Accessory Gland    135.2 Adult Carcass    64.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    183.8 Larval Midgut    49.8 Larval Hindgut    58.2 Larval Malpighian Tubules    97.4 Larval Fat Body    93.3 Larval Salivary Gland    151.1 Larval Trachea    228.225 Larval Carcass    66.775 Adult Head    66.8 Adult Eye    127.475 Adult Brain    146.5 Adult Thoracic-Abdominal Ganglion    98.7 Adult Crop    94.3 Adult Midgut    77.9 Adult Hindgut    64.4 Adult Malpighian Tubules    98.5 Adult Fat Body    60.9 Adult Salivary Gland    100.8 Adult Heart    93 Adult VirginFemale Spermatheca    96.3 Adult InseminatedFemale Spermatheca    91 Adult Ovary    334.3 Adult Testis    33.3 Adult Male Accessory Gland    135.2 Adult Carcass    64.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    183.8 Larval Midgut    49.8 Larval Hindgut    58.2 Larval Malpighian Tubules    97.4 Larval Fat Body    93.3 Larval Salivary Gland    151.1 Larval Trachea    228.225 Larval Carcass    66.775 Adult Head    66.8 Adult Eye    127.475 Adult Brain    146.5 Adult Thoracic-Abdominal Ganglion    98.7 Adult Crop    94.3 Adult Midgut    77.9 Adult Hindgut    64.4 Adult Malpighian Tubules    98.5 Adult Fat Body    60.9 Adult Salivary Gland    100.8 Adult Heart    93 Adult VirginFemale Spermatheca    96.3 Adult InseminatedFemale Spermatheca    91 Adult Ovary    334.3 Adult Testis    33.3 Adult Male Accessory Gland    135.2 Adult Carcass    64.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    66.8   NA  Eye    127.475   NA  Brain    146.5   183.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    98.7   NA  Crop    94.3   49.8  Midgut    77.9   58.2  Hindgut    64.4   97.4  Malpighian Tubules    98.5   93.3  Fat Body    60.9   151.1  Salivary Gland    100.8   NA  Heart    93   228.225  Trachea    NA   NA  VirginFemale Spermatheca    96.3   NA  InseminatedFemale Spermatheca    91   NA  Ovary    334.3   NA  Testis    33.3   NA  Male Accessory Gland    135.2   66.775  Carcass    64.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    25 central nervous system, pupae P8    8 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    3 digestive system, 4-day adult    6 digestive system, 20-day adult    5 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    12 carcass, 1-day adult    4 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    75 ovary, mated 4-day female    64 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    25 central nervous system, pupae P8    8 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    3 digestive system, 4-day adult    6 digestive system, 20-day adult    5 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    12 carcass, 1-day adult    4 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female  (75) ovary, mated 4-day female  (64) testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    25 central nervous system, pupae P8    8 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    3 digestive system, 4-day adult    6 digestive system, 20-day adult    5 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    12 carcass, 1-day adult    4 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    75 ovary, mated 4-day female    64 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    25 central nervous system, pupae P8    8 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    3 digestive system, 4-day adult    6 digestive system, 20-day adult    5 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    12 carcass, 1-day adult    4 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    75 ovary, mated 4-day female    64 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    25 central nervous system, pupae P8    8 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    3 digestive system, 4-day adult    6 digestive system, 20-day adult    5 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    12 carcass, 1-day adult    4 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    75 ovary, mated 4-day female    64 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    25 central nervous system, pupae P8    8 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    3 digestive system, 4-day adult    6 digestive system, 20-day adult    5 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    12 carcass, 1-day adult    4 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female  (75) ovary, mated 4-day female  (64) testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    25 central nervous system, pupae P8    8 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    3 digestive system, 4-day adult    6 digestive system, 20-day adult    5 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    12 carcass, 1-day adult    4 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    75 ovary, mated 4-day female    64 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    25 central nervous system, pupae P8    8 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    3 digestive system, 4-day adult    6 digestive system, 20-day adult    5 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    12 carcass, 1-day adult    4 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    75 ovary, mated 4-day female    64 testis, mated 4-day male    6 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    87 embryo 02-04hr    22 embryo 04-06hr    20 embryo 06-08hr    25 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    5 embryo 22-24hr    5 larva L1    8 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    22 adult female 05day    41 adult female 30day    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (87) embryo 02-04hr    22 embryo 04-06hr    20 embryo 06-08hr    25 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    5 embryo 22-24hr    5 larva L1    8 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    22 adult female 05day  (41) adult female 30day  (44) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    87 embryo 02-04hr    22 embryo 04-06hr    20 embryo 06-08hr    25 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    5 embryo 22-24hr    5 larva L1    8 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    22 adult female 05day    41 adult female 30day    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    87 embryo 02-04hr    22 embryo 04-06hr    20 embryo 06-08hr    25 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    5 embryo 22-24hr    5 larva L1    8 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    22 adult female 05day    41 adult female 30day    44 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    87 embryo 02-04hr    22 embryo 04-06hr    20 embryo 06-08hr    25 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    5 embryo 22-24hr    5 larva L1    8 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    22 adult female 05day    41 adult female 30day    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (87) embryo 02-04hr    22 embryo 04-06hr    20 embryo 06-08hr    25 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    5 embryo 22-24hr    5 larva L1    8 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    22 adult female 05day  (41) adult female 30day  (44) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    87 embryo 02-04hr    22 embryo 04-06hr    20 embryo 06-08hr    25 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    5 embryo 22-24hr    5 larva L1    8 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    22 adult female 05day    41 adult female 30day    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    87 embryo 02-04hr    22 embryo 04-06hr    20 embryo 06-08hr    25 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    5 embryo 22-24hr    5 larva L1    8 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    8 adult female 01day    22 adult female 05day    41 adult female 30day    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    18 Schneider line 2 Sg4    14 embryonic 1182-4H    13 embryonic GM2    20 embryonic Kc167    13 embryonic S1    14 embryonic S3    15 leg disc CME L1    15 wing disc CME-W2    13 wing disc ML-DmD8    14 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    11 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    23 ovary OSC    13 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    18 Schneider line 2 Sg4    14 embryonic 1182-4H    13 embryonic GM2    20 embryonic Kc167    13 embryonic S1    14 embryonic S3    15 leg disc CME L1    15 wing disc CME-W2    13 wing disc ML-DmD8    14 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    11 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    23 ovary OSC    13 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    18 Schneider line 2 Sg4    14 embryonic 1182-4H    13 embryonic GM2    20 embryonic Kc167    13 embryonic S1    14 embryonic S3    15 leg disc CME L1    15 wing disc CME-W2    13 wing disc ML-DmD8    14 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    11 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    23 ovary OSC    13 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    18 Schneider line 2 Sg4    14 embryonic 1182-4H    13 embryonic GM2    20 embryonic Kc167    13 embryonic S1    14 embryonic S3    15 leg disc CME L1    15 wing disc CME-W2    13 wing disc ML-DmD8    14 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    11 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    23 ovary OSC    13 ovary OSS    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    18 Schneider line 2 Sg4    14 embryonic 1182-4H    13 embryonic GM2    20 embryonic Kc167    13 embryonic S1    14 embryonic S3    15 leg disc CME L1    15 wing disc CME-W2    13 wing disc ML-DmD8    14 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    11 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    23 ovary OSC    13 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    18 Schneider line 2 Sg4    14 embryonic 1182-4H    13 embryonic GM2    20 embryonic Kc167    13 embryonic S1    14 embryonic S3    15 leg disc CME L1    15 wing disc CME-W2    13 wing disc ML-DmD8    14 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    11 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    23 ovary OSC    13 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    18 Schneider line 2 Sg4    14 embryonic 1182-4H    13 embryonic GM2    20 embryonic Kc167    13 embryonic S1    14 embryonic S3    15 leg disc CME L1    15 wing disc CME-W2    13 wing disc ML-DmD8    14 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    11 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    23 ovary OSC    13 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    18 Schneider line 2 Sg4    14 embryonic 1182-4H    13 embryonic GM2    20 embryonic Kc167    13 embryonic S1    14 embryonic S3    15 leg disc CME L1    15 wing disc CME-W2    13 wing disc ML-DmD8    14 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    11 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    23 ovary OSC    13 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    12 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    52 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    12 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult  (48) Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3  (52) Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (42) Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult  (34) Paraquat 10 mM 48 hrs, 4-day adult  (28) Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    12 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    52 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    12 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    52 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    12 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    52 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    12 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult  (48) Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3  (52) Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (42) Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult  (34) Paraquat 10 mM 48 hrs, 4-day adult  28 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    12 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    52 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    12 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    52 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD31045 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035997 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3445-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | germline clone | maternal effect | recessive phol81A phol106C lethal | embryonic stage | maternal effect | recessive phol81A phol106C viable phol81A phol106C viable, with Scer\GAL4[-]: pholEP559 viable, with Scer\GAL4pnr-MD237 pholGD10970 Sterility Allele female sterile | recessive phol81A phol106C fertile, with Scer\GAL4[-]: pholEP559 male fertile phol81A phol106C Phenotype manifest in Allele
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of phol Class Mutagen Stocks Known lesion pholKG10524 P-element activity 2 -- phol81A Delta2-3 1 Yes pholEP559 P-element activity 1 Yes phol106C Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of phol Class Mutagen Stocks Known lesion pholGD10970 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10970} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}pholEP559 NA P{GSV1}C403C403 NA miscellaneous insertions P{?EP}phol81A NA P{SUPor-P}pholKG10524 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding inferred from direct assay (Brown et al., 2003)
Biological Process
CV term Evidence References
gene silencing inferred from genetic interaction with pho (Brown et al., 2003)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Klymenko et al., 2006)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
DNA binding traceable author statement (Levine et al., 2004) zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions phol allele Gene References phol81A pho (Brown et al., 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64488 DroID - A comprehensive database of gene and protein interactions. • FBgn0035997 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39095 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VSZ3
Orthologs
OrthoDB Orthologs (41) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6BVSD6 Dipteran inclusive ortholog search EOG68KRKG Insect inclusive ortholog search EOG6NZSPT Arthropod inclusive ortholog search EOG6VT4CS Metazoa inclusive ortholog search EOG63581D
Orthologs in Drosophila Species (EOG6BVSD6)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\phol     Drosophila simulans   Dsim\GD12921 Y   Drosophila sechellia   Dsec\GM24870 Y   Drosophila erecta   Dere\GG14036 Y   Drosophila yakuba   Dyak\GE21239 Y   Drosophila ananassae   Dana\GF24664 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17456 Y   Drosophila persimilis   Dper\GL17968 Y   Drosophila willistoni   Dwil\GK20506 Y   Drosophila virilis   Dvir\GJ13188 Y   Drosophila mojavensis   Dmoj\GI13359 Y   Drosophila grimshawi   Dgri\GH16213 Y
Orthologs in non-Drosophila Dipterans (EOG68KRKG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ014689 Y
Orthologs in non-Dipteran Insects (EOG6NZSPT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49343 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG000131 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12483 Y Atta cephalotes Leafcutter ant  Acep\ACEP14480 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO24325 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO25340 Y Linepithema humile Argentine ant  Lhum\LH14355 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB23302 Y Solenopsis invicta Red fire ant  Sinv\SINV16765 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI007734 Y Bombyx mori Silkmoth  Bmor\BGIBMGA011179 Y Pediculus humanus Human body louse  Phum\PHUM284920 Y Tribolium castaneum Red flour beetle  Tcas\pho Y
Orthologs in non-Insect Arthropods (EOG6VT4CS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\YY1 Y Ixodes scapularis Deer tick  Isca\ISCW010258 Y
Orthologs in non-Arthropod Metazoa (EOG63581D)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_010295gn Y Danio rerio Zebrafish  Drer\yy1b Y Danio rerio Zebrafish  Drer\yy1a Y Xenopus tropicalis Western clawed frog  Xtro\yy1 Y Gallus gallus Domestic chicken  Ggal\YY1 Y Mus musculus House mouse  Mmus\Yy1 Y Mus musculus House mouse  Mmus\Zfp42 Y Mus musculus House mouse  Mmus\Yy2 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000033956 Y Rattus norvegicus Norway rat  Rnor\Yy1 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032977 Y Homo sapiens Human  Hsap\YY2 Y Homo sapiens Human  Hsap\ZFP42 Y
Human Orthologs (2)
Gene OMIM HGNC Hsap\YY2     Hsap\ZFP42
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD12921 Drosophila sechellia Dsec\GM24870 Drosophila erecta Dere\GG14036 Drosophila yakuba Dyak\GE21239 Drosophila ananassae Dana\GF24664 Drosophila pseudoobscura pseudoobscura Dpse\GA17456 Drosophila persimilis Dper\GL17968 Drosophila willistoni Dwil\GK20506 Drosophila virilis Dvir\GJ13188 Drosophila mojavensis Dmoj\GI13359 Drosophila grimshawi Dgri\GH16213
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	17180 w1118; P{EP}pholEP559 24164 y1 w*; phol81A/TM3, Ser1 y+ 15275 y1; P{SUPor-P}pholKG10524 ry506/TM3, Sb1 Ser1
Kyoto	111700 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{SUPor-P}pholKG10524 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb1
VDRC	v26196 w1118; P{GD10970}v26196
Genomic Clones ( 1 )
	BACR01E20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 33 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD31045
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT12158 AT19916 GH04307 GH15016 HL06538 LD36526 LD41543 LD42095 RE17668 RE68434 RH05144 RH12302 SD12915
Other clones	7585 43649 59519 108792 191117 Dmel_neb_015075_0688_2683 EK125640 EK162920 EK186426 EK235245 EK294056 RP003039733 RP003058612 RP003154179 RP003159508 RP003173540 RP003362213 RP003473314 RP003648227
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0035997 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39095
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: phol CG3445 (Kassis, 2003.2.10)
Source for database merge of
Additional comments
Other Comments
	DNA-protein interactions: genome-wide binding profile assayed for phol protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069). (Filion et al., 2010) pho and phol act redundantly to repress homeotic genes. (Brown et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140060 RefSeq (Proteins) • NP_648317 DDBJ / EMBL / GenBank • AA950886 AAF50265 AAM50261 AI516282 AQ025112 AW943062 AY119607 BZ665776 Entrez Gene - A searchable database of RefSeq genes. • 39095 UniProtKB/TrEMBL • Q9VSZ3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger C2H2-type/integrase DNA-binding domain (IPR013087) Zinc finger, C2H2-like (IPR015880) Zinc finger, C2H2 (IPR007087) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD31045
Linkouts
	BioGRID - A database of protein and genetic interactions • 64488 DroID - A comprehensive database of gene and protein interactions. • FBgn0035997 DRSC - Results from RNAi screens. • FBgn0035997 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0035997 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3445-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0035997 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39095 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39095 modMine - Data generated by the modENCODE project. • FBgn0035997 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VSZ3
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	CG3445 (Molnar et al., 2006) phol (Gan et al., 2010, Molnar et al., 2006, Savla et al., 2008, Fujioka et al., 2008, Kim et al., 2008, Morozova et al., 2009, Takács et al., 2012) PHOL (Schwartz and Pirrotta, 2007, Savla et al., 2008, Schuettengruber et al., 2009, Filion et al., 2010) Phol (Brown et al., 2005, Klymenko et al., 2006, Huen and Russell, 2010)
Name Synonym	pho-like (Lund and van Lohuizen, 2004, Lehmann, 2004) Pho-like (Klymenko et al., 2006, Muller and Kassis, 2006, Ringrose and Paro, 2007) PHO-LIKE (Savla et al., 2008) pleiohomeotic like (Kassis et al., 2003, ) Pleiohomeotic like (Brown et al., 2005) pleiohomeotic-like (Kim et al., 2008) Pleiohomeotic-like (Schuettengruber et al., 2009, Jacques et al., 2009, Huen and Russell, 2010)
Secondary FlyBase IDs
References ( 37 )
Generate a list of	All references relating to this gene ( 37 )
List References by type	Research paper  ( 15 ) Supplementary material  ( 4 ) Review  ( 6 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 2 ) FlyBase analysis  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 1 )
	Takács et al., 2012, Int. J. Biol. Sci. 8(7): 1055--1061 Protein interactions on telomeric retrotransposons in Drosophila. [FBrf0219367] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...