evi, srt, sprinter, Evi/Wls, evenness interrupted
novel conserved transmembrane protein that acts in the Wnt-sending cells to promote the secretion of Wnt proteins - mutation triggers the retrograde Golgi-to-ER transport of Wingless and induces ER stress
Gene model reviewed during 5.45
None of the polypeptides share 100% sequence identity.
Interacts with wg; in the Golgi. Interacts with Vps35, a component of the retromer complex; wls stability is regulated by Vps35.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\wls using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\wls in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
wls protein accumulates in the Golgi apparatus of wg-producing cells, and this localisation is essential for wg protein secretion. wls protein localisation and levels critically depend on the retromer complex.
dsRNA made from templates generated with primers against the 3' UTR and against the ORF of this gene.
Mutations in srt prevent the secretion of active wg ligand in signal sending cells; comparable levels of wg protein are present outside of srt mutant and wild-type cells, however, wg protein produced in srt mutant cells cannot be taken up into endocytic vesicles in receiving cells.