FB2025_01 , released February 20, 2025
Gene: Dmel\CG7394
Open Close
General Information
Symbol
Dmel\CG7394
Species
D. melanogaster
Name
Annotation Symbol
CG7394
Feature Type
protein_coding_gene
FlyBase ID
FBgn0036173
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Summary
Probable component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity (By similarity). (UniProt, Q9VTJ8)
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
68C13-68C13
Sequence location
Recombination map
3-36
RefSeq locus
NT_037436 REGION:11561996..11564312
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables ATPase activator activity
inferred from biological aspect of ancestor with PANTHER:PTN000298641
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:Q07914
(Gene Ontology Curators, 2002-)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in protein import into mitochondrial matrix
inferred from sequence or structural similarity with UniProtKB:Q07914
(Gene Ontology Curators, 2002-)
inferred from biological aspect of ancestor with PANTHER:PTN000298641
(GO Reference Genome Project, 2011-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of PAM complex, Tim23 associated import motor
inferred from sequence or structural similarity with UniProtKB:Q07914
(Gene Ontology Curators, 2002-)
inferred from biological aspect of ancestor with PANTHER:PTN000298641
(GO Reference Genome Project, 2011-)
part_of TIM23 mitochondrial import inner membrane translocase complex
inferred from sequence or structural similarity with UniProtKB:Q07914
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the TIM14 family. (Q9VTJ8)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
HEAT SHOCK PROTEIN 40/DNAJ CO-CHAPERONES -
The Heat Shock Protein 40 (Hsp40) family of co-chaperones are essential factors in the Hsp70 chaperone cycle. The Hsp40 family is diverse in size and structure, but a number of them are about 40kDa and have been identified as products of heat shock genes. Their defining feature is the DnaJ or J-domain corresponding to the N-terminal 70 amino acids of the Escherichia coli co-chaperone DnaJ. (Adapted from FBrf0173155).
PRESEQUENCE TRANSLOCASE-ASSOCIATED MOTOR SUBUNITS -
The presequence translocase-associated motor (PAM) is a subcomplex of the TIM23 complex. It is required to drive the ATP hydrolysis-dependent translocation of proteins across the inner mitochondrial membrane for import into the matrix. (Adapted from PMID:32074073.)
Protein Function (UniProtKB)
Probable component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity (By similarity).
(UniProt, Q9VTJ8)
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\CG7394 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VTJ8)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
CG7394-RA
FBtr0076098
NM_140218
534
118
CG7394-RB
FBtr0290024
NM_001144461
890
128
CG7394-RC
FBtr0305648
NM_001259788
1315
118
CG7394-RD
FBtr0333215
NM_001274785
1431
118
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
CG7394-PA
FBpp0075829
12.9
118
10.63
Q9VTJ8
NP_648475
AAF50050
CG7394-PB
FBpp0288463
14.0
128
10.62
B7Z0G3
NP_001137933
ACL83288
CG7394-PC
FBpp0296928
12.9
118
10.63
Q9VTJ8
NP_001246717
AFH04388
CG7394-PD
FBpp0305417
12.9
118
10.63
Q9VTJ8
NP_001261714
AGB94408
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

118 aa isoforms: CG7394-PA, CG7394-PC, CG7394-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, Roe1, TIM44, blp/TIM16 and TIM14.

(UniProt, Q9VTJ8)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG7394 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

4.65

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG7394 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of CG7394
Transgenic constructs containing regulatory region of CG7394
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (10)
Hsap\DNAJC19
13 of 14
Yes
Yes
1  
Hsap\DNAJC15
10 of 14
No
Yes
Hsap\DNAJB5
1 of 14
No
No
1  
Hsap\DNAJC4
1 of 14
No
No
Hsap\DNAJC9
1 of 14
No
No
Hsap\DNAJC12
1 of 14
No
No
Hsap\DNAJC17
1 of 14
No
No
Hsap\DNAJC24
1 of 14
No
No
Hsap\DNAJC27
1 of 14
No
No
Hsap\DNAJC30
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (9)
Rnor\Dnajc15
12 of 14
Yes
Yes
Rnor\Dnajc19
9 of 14
No
Yes
Rnor\Dnaja4
1 of 14
No
No
Rnor\Dnajb5
1 of 14
No
No
Rnor\Dnajc4
1 of 14
No
No
Rnor\Dnajc9
1 of 14
No
No
Rnor\Dnajc17
1 of 14
No
No
Rnor\Dnajc27
1 of 14
No
No
Rnor\Dnajc30
1 of 14
No
No
Mus musculus (laboratory mouse) (12)
Mmus\Dnajc19
12 of 14
Yes
Yes
Mmus\Dnajc15
9 of 14
No
Yes
Mmus\Dnajc19-ps
2 of 14
No
Yes
Mmus\Dnajb5
1 of 14
No
No
Mmus\Dnajc4
1 of 14
No
No
Mmus\Dnajc7
1 of 14
No
No
Mmus\Dnajc9
1 of 14
No
No
Mmus\Dnajc12
1 of 14
No
No
Mmus\Dnajc17
1 of 14
No
No
Mmus\Dnajc24
1 of 14
No
No
Mmus\Dnajc27
1 of 14
No
No
Mmus\Dnajc30
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\dnajc19
11 of 13
Yes
Yes
Xtro\dnajc15
6 of 13
No
Yes
Xtro\LOC100495396
1 of 13
No
No
Danio rerio (Zebrafish) (9)
Drer\dnajc19
13 of 14
Yes
Yes
Drer\dnajc15
10 of 14
No
Yes
Drer\dnajc4
1 of 14
No
No
Drer\dnajc7
1 of 14
No
No
Drer\dnajc9
1 of 14
No
No
Drer\dnajc12
1 of 14
No
No
Drer\dnajc27
1 of 14
No
No
Drer\dnajc30b
1 of 14
No
No
Drer\pam16
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (5)
Cele\dnj-21
14 of 14
Yes
Yes
Cele\dnj-16
1 of 14
No
Yes
Cele\dnj-22
1 of 14
No
No
Cele\dnj-23
1 of 14
No
No
Cele\K07F5.16
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (6)
Agam\AgaP_AGAP003533
12 of 12
Yes
Yes
Agam\AgaP_AGAP000970
1 of 12
No
No
Agam\AgaP_AGAP002386
1 of 12
No
No
Agam\AgaP_AGAP003825
1 of 12
No
No
Agam\AgaP_AGAP004849
1 of 12
No
No
Agam\AgaP_AGAP007361
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (15)
Atha\AT2G35795
13 of 13
Yes
Yes
Atha\AT3G09700
13 of 13
Yes
Yes
Atha\AT5G03030
13 of 13
Yes
Yes
Atha\ARL2
1 of 13
No
No
Atha\AT1G21080
1 of 13
No
No
Atha\AT1G76700
1 of 13
No
No
Atha\AT1G77020
1 of 13
No
No
Atha\AT2G21510
1 of 13
No
No
Atha\AT3G12170
1 of 13
No
No
Atha\AT4G39150
1 of 13
No
No
Atha\AT5G23590
1 of 13
No
No
Atha\ATJ6
1 of 13
No
No
Atha\OWL1
1 of 13
No
No
Atha\TPR16
1 of 13
No
No
Atha\TXR1
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (5)
Scer\PAM18
9 of 13
Yes
Yes
Scer\MDJ2
8 of 13
No
Yes
Scer\CAJ1
1 of 13
No
No
Scer\DJP1
1 of 13
No
No
Scer\SIS1
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (6)
Spom\tim14
12 of 12
Yes
Yes
Spom\cwf23
1 of 12
No
No
Spom\SPAC4H3.01
1 of 12
No
No
Spom\SPAC1071.09c
1 of 12
No
No
Spom\SPBC3E7.11c
1 of 12
No
No
Spom\tim16
1 of 12
No
No
Escherichia coli (enterobacterium) (3)
Ecol\djlB
1 of 11
Yes
Yes
Ecol\djlC
1 of 11
Yes
Yes
Ecol\dnaJ
1 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:CG7394. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (8)
CG32727
4 of 13
CG1409
1 of 13
CG6693
1 of 13
CG14650
1 of 13
CG17187
1 of 13
CG32640
1 of 13
CG32641
1 of 13
jdp
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    DNAJC19; DnaJ heat shock protein family (Hsp40) member C19
    13 of 14
    608977
     
    DNAJC15; DnaJ heat shock protein family (Hsp40) member C15
    10 of 14
    615339
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Other Interaction Browsers
        Summary of Genetic Interactions
        esyN Network Diagram
        Other Interaction Browsers
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, Roe1, TIM44, blp/TIM16 and TIM14.
        (UniProt, Q9VTJ8 )
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3L
        Recombination map
        3-36
        Cytogenetic map
        68C13-68C13
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        68C13-68C13
        Limits computationally determined from genome sequence between P{PZ}l(3)0123901239&P{lacW}l(3)01239j9B4 and P{PZ}CycA02461&P{PZ}CycA03946
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        3-36

        (FlyBase, 2016)

        3-30.0

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (5)
        Genomic Clones (13)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (96)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments
              Relationship to Other Genes
              Source for database merge of

              Source for merge of: CG7394 BcDNA:RE52392

              (FlyBase, 1996-)
              Additional comments

              Source for merge of CG7394 BcDNA:RE52392 was a shared cDNA ( date:030728 ).

              (FlyBase, 1996-)
              Nomenclature History
              Source for database identify of
              Nomenclature comments
              Etymology
              Synonyms and Secondary IDs (4)
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              Study result (0)
              Result
              Result Type
              Title
              External Crossreferences and Linkouts ( 43 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
              FlyMine - An integrated database for Drosophila genomics
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              MARRVEL_MODEL - MARRVEL (model organism gene)
              Linkouts
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              References (45)