FlyBase Gene Report: Dmel\Ncc69

General Information

Symbol

Dmel\Ncc69

Species

D. melanogaster

Name

sodium chloride cotransporter 69

Annotation Symbol

CG4357

Feature Type

protein_coding_gene

FlyBase ID

FBgn0036279

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Summary

sodium chloride cotransporter 69 (Ncc69) encodes a member of the SLC12 family of Na[+]/K[+]/2Cl[-] cotransporters (NKCC). [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

69B3-69B3

Sequence location

3L:12,368,646..12,391,444 [-]

Recombination map

3-38

RefSeq locus

NT_037436 REGION:12368646..12391444

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables sodium:potassium:chloride symporter activity

inferred from direct assay

(Leiserson et al., 2011, Sun et al., 2010)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables amino acid transmembrane transporter activity

inferred from sequence or structural similarity with FLYBASE:Eaat1; FB:FBgn0026439

(Romero-Calderon et al., 2007)

enables potassium:chloride symporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000214719

(assigned by GO_Central )

(Gaudet et al., 2011)

enables sodium:potassium:chloride symporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000930537

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (10 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in chloride transport

inferred from direct assay

(Sun et al., 2010)

involved_in potassium ion transport

inferred from direct assay

(Sun et al., 2010)

inferred from mutant phenotype

(Wu et al., 2014)

Terms Based on Predictions or Assertions (8 terms)

CV Term

Evidence

References

involved_in amino acid transmembrane transport

inferred from sequence or structural similarity with FLYBASE:Eaat1; FB:FBgn0026439

(Romero-Calderon et al., 2007)

involved_in cell volume homeostasis

inferred from biological aspect of ancestor with PANTHER:PTN000214719

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in chloride ion homeostasis

inferred from biological aspect of ancestor with PANTHER:PTN000214719

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in chloride transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000214719

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in potassium ion homeostasis

inferred from biological aspect of ancestor with PANTHER:PTN000214719

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in potassium ion import across plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000214719

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in sodium ion homeostasis

inferred from biological aspect of ancestor with PANTHER:PTN000930537

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in sodium ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000930537

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Sun et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in integral component of membrane

inferred from electronic annotation with InterPro:IPR004842

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Gene Group (FlyBase)

SLC12 FAMILY OF CATION-COUPLED CHLORIDE TRANSPORTERS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

sodium chloride cotransporter 69 (Ncc69) encodes a member of the SLC12 family of Na[+]/K[+]/2Cl[-] cotransporters (NKCC). [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

SLC12 FAMILY OF CATION-COUPLED CHLORIDE TRANSPORTERS -: Solute carrier family 12 (SLC12) members are sodium and/or potassium-coupled chloride transporters. (Adapted from FBrf0209627).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ncc69 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry M9PI37)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Gene model reviewed during 6.02

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ncc69-RA

FBtr0075987

NM_140315

3936

1171

Ncc69-RC

FBtr0333838

NM_001274827

4822

1191

Ncc69-RD

FBtr0333839

NM_001274828

3996

1191

Ncc69-RE

FBtr0333840

NM_001274829

4044

1207

Ncc69-RF

FBtr0333841

NM_001274830

4051

1191

Ncc69-RG

FBtr0333842

NM_206344

4762

1171

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ncc69-PA

FBpp0075719

129.2

1171

6.82

NP_648572

AAF49927

Ncc69-PC

FBpp0305972

131.6

1191

6.99

NP_001261756

AGB94449

Ncc69-PD

FBpp0305973

131.6

1191

6.99

NP_001261757

AGB94450

Ncc69-PE

FBpp0305974

133.5

1207

7.77

NP_001261758

AGB94451

Ncc69-PF

FBpp0305975

131.6

1191

6.99

NP_001261759

AGB94452

Ncc69-PG

FBpp0305976

129.2

1171

6.82

NP_996066

AAS65014

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1171 aa isoforms: Ncc69-PA, Ncc69-PG

1191 aa isoforms: Ncc69-PC, Ncc69-PD, Ncc69-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ncc69 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 6

organism

(Sun et al., 2010)

embryonic stage 9 -- 13

embryonic/larval midgut primordium

(Sun et al., 2010)

presumptive embryonic/larval central nervous system

(Sun et al., 2010)

embryonic stage 14 -- 17

embryonic/larval foregut

(Sun et al., 2010)

embryonic stage 16

embryonic/larval CNS subperineurial glial cell

(Leiserson et al., 2011)

dorsal median cell

(Leiserson et al., 2011)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

(Romero-Calderon et al., 2007)

adult thorax | faint

Comment: Thorax and abdomen assayed together.

(Romero-Calderon et al., 2007)

adult abdomen | faint

Comment: Thorax and abdomen assayed together.

(Romero-Calderon et al., 2007)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | faint

(Filippov et al., 2003)

adult stage

adult midgut | faint

(Filippov et al., 2003)

adult head | faint

(Filippov et al., 2003)

adult stage | male

adult thorax | faint & adult abdomen | faint

Comment: reference stages "body"

(Filippov et al., 2003)

adult stage | female

adult thorax | faint & adult abdomen | faint

Comment: reference stages "body"

(Filippov et al., 2003)

Additional Descriptive Data

Ncc69 is expressed in subperineurial glial cells. The only other prominent expression of Ncc69 is in the dorsal median cells of the ventral ganglion.

(Leiserson et al., 2011)

During embyronic stages 9-13, expression is seen in the developing gut and nervous system. In later stages, expression is predominantly in the foregut and anterior portion of the embryo.

(Sun et al., 2010)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult lamina satellite glial cell

(Stenesen et al., 2019)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Ncc69 protein is expressed in glial cells. In the adult eye, Ncc69 protein expression is enriched in satellite glial cells in the distal lamina.

(Stenesen et al., 2019)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Sun et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ncc69 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ncc69

View exonic expression by tissue for Dmel\Ncc69

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39410/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036279

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036279

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036279

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4357

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ncc69

Transgenic constructs containing regulatory region of Ncc69

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)eyg^C1

(Sun et al., 2010)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ncc69^GD14863

viable, with Scer\GAL4^Mef2.PR

Ncc69^GD14863

viable, with Scer\GAL4^pnr-MD237

Ncc69^GD14863

viable, with Scer\GAL4^tin.CΔ4

Ncc69^GD14863

viable (with Df(3L)eyg^C1)

Ncc69^DG04311

Sterility

Allele

fertile

Ncc69^PL00618

Other Phenotypes

Allele

abnormal locomotor behavior | first instar larval stage, with Scer\GAL4^repo.PU

Ncc69^UAS.Tag:HA

abnormal neuroanatomy, with Scer\GAL4^repo.PU

Ncc69^GD14863

abnormal neuroanatomy | first instar larval stage, with Scer\GAL4^repo.PU

Ncc69^UAS.Tag:HA

abnormal neuroanatomy | third instar larval stage

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Gli-rL82.29

Ncc69^GD14863

bang sensitive, with Scer\GAL4^repo.PU

paralytic, with Scer\GAL4^repo.PU

Phenotype manifest in

Allele

abdominal nerve | larval stage

Ncc69^r1

abdominal nerve | third instar larval stage

Ncc69^r2

abdominal nerve | third instar larval stage, with Scer\GAL4^Gli-rL82.29

Ncc69^GD14863

abdominal nerve | third instar larval stage, with Scer\GAL4^repo.PU

Ncc69^GD14863

astrocyte-like glial cell | first instar larval stage, with Scer\GAL4^repo.PU

Ncc69^UAS.Tag:HA

neuropil | first instar larval stage, with Scer\GAL4^repo.PU

Ncc69^UAS.Tag:HA

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (22)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SLC12A2

15 of 15

Yes

Hsap\SLC12A1

13 of 15

Yes

Hsap\SLC12A3

12 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (25)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Slc12a2

15 of 15

Yes

Mmus\Slc12a1

12 of 15

Yes

Mmus\Slc12a3

12 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (25)

Rnor\Slc12a1

12 of 13

Yes

Rnor\Slc12a2

10 of 13

Yes

Rnor\Slc12a3

8 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (23)

Xtro\slc12a1

7 of 12

Yes

Xtro\slc12a3

6 of 12

Yes

Xtro\slc12a2

3 of 12

Yes

Xtro\slc12a3.2

2 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (34)

Drer\slc12a3

11 of 15

Yes

Drer\slc12a10.2

9 of 15

Yes

Drer\slc12a10.3

8 of 15

Yes

Drer\slc12a2

7 of 15

Yes

Drer\slc12a10.1

7 of 15

Yes

Drer\slc12a1

6 of 15

Yes

Drer\LOC100537771

2 of 15

Yes

Drer\si:cabz01090193.1

2 of 15

Drer\si:ch211-59d13.2

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Yes

Drer\si:cabz01102082.1

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (20)

Cele\nkcc-1

13 of 15

Yes

Cele\B0303.11

4 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (1)

Atha\CCC1

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (25)

Scer\AGP1

1 of 15

Yes

Scer\AGP2

1 of 15

Yes

Scer\AGP3

1 of 15

Yes

Scer\ALP1

1 of 15

Yes

Scer\BAP2

1 of 15

Yes

Scer\BAP3

1 of 15

Yes

Scer\BIO5

1 of 15

Yes

Scer\CAN1

1 of 15

Yes

Scer\DIP5

1 of 15

Yes

Scer\GAP1

1 of 15

Yes

Scer\GNP1

1 of 15

Yes

Scer\HIP1

1 of 15

Yes

Scer\HNM1

1 of 15

Yes

Scer\LYP1

1 of 15

Yes

Scer\MMP1

1 of 15

Yes

Scer\MUP1

1 of 15

Yes

Scer\MUP3

1 of 15

Yes

Scer\PUT4

1 of 15

Yes

Scer\SAM3

1 of 15

Yes

Scer\SSY1

1 of 15

Yes

Scer\TAT1

1 of 15

Yes

Scer\TAT2

1 of 15

Yes

Scer\TPO5

1 of 15

Yes

Scer\UGA4

1 of 15

Yes

Scer\VHC1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (15)

Gene Symbol

Score

Source

Alignment

6 of 10

3 of 10

3 of 10

kcc

3 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

mnd

1 of 10

sbm

1 of 10

slif

1 of 10

tadr

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

seizure-sensitive (postulated), SLC12A1-3-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Ncc69^GD14863

model of epilepsy

CEA

(Rusan et al., 2014)

Ncc69^JF03097

model of epilepsy

CEA

(Rusan et al., 2014)

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

SLC12A2; solute carrier family 12 member 2

model of autosomal dominant nonsyndromic deafness 78

IEA

(FlyBase, 2019-)

SLC12A1; solute carrier family 12 member 1

model of Bartter disease type 1

IEA

(FlyBase, 2019-)

SLC12A3; solute carrier family 12 member 3

model of Gitelman syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Ncc69^UAS.Tag:HA

ameliorates episodic ataxia type 6

modeled by Hsap\SLC1A3^PR.UAS.Venus

(Parinejad et al., 2016)

modeled by Eaat1^PR.UAS.Venus

(Parinejad et al., 2016)

Ncc69^JF03097

exacerbates NGLY1-deficiency

modeled by Pngl^HMS02424

(Talsness et al., 2020)

modeled by Pngl^HMJ21590

(Talsness et al., 2020)

ameliorates glioblastoma

modeled by Pi3K92E^CAAX.UAS, btl::Egfr^λ.UAS

(Chi et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC12A2; solute carrier family 12 member 2

15 of 15

600840

autosomal dominant nonsyndromic deafness 78

SLC12A1; solute carrier family 12 member 1

13 of 15

600839

BARTTER SYNDROME, TYPE 1, ANTENATAL; BARTS1

Bartter disease type 1

SLC12A3; solute carrier family 12 member 3

12 of 15

600968

GITELMAN SYNDROME; GTLMNS

Gitelman syndrome

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ncc69 - fray

two hybrid, enzymatic study, autoradiography

(Sun et al., 2018, Leiserson et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Eaat1

suppressible

Ncc69

(Parinejad et al., 2016)

kcc

suppressible

Ncc69

(Hekmat-Scafe et al., 2010)

Wnk

suppressible

Ncc69

(Wu et al., 2014)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64762

DroID - A comprehensive database of gene and protein interactions.

FBgn0036279

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0036279

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Cation-coupled Chloride cotransporters

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-38

Cytogenetic map

69B3-69B3

Sequence location

3L:12,368,646..12,391,444 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

69B3-69B3

Limits computationally determined from genome sequence between P{PZ}l(3)05088⁰⁵⁰⁸⁸ and P{PZ}l(3)06924⁰⁶⁹²⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-38

(FlyBase, 2016)

3-31.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

24611

w¹¹¹⁸; Mi{ET1}Ncc69^MB05292

28682

y¹ v¹; P{TRiP.JF03097}attP2

59591

y¹ w^*; Mi{MIC}Ncc69^MI15026

19568

w^*; PBac{GAL4D,EYFP}Ncc69^PL00618 P{FRT(w^hs)}2A P{neoFRT}82B

VDRC

v106499

P{KK108763}VIE-260B

v30000

w¹¹¹⁸; P{GD14863}v30000

Genomic Clones (32)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (25)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_GH15356

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0036279

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0036279

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39410

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Sun et al., 2010)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Ncc69 CG4357

(Leiserson, 2005.3.2)

Source for database merge of

Source for merge of: CG4357 BEST:CK01027

(Rana, 2004.7.8)

Additional comments

Other Comments

Identification: as a protein that physically interacts with the N-terminal region of the fray gene product.

(Leiserson and Keshishian, 2005)

Identification: cDNA screen for secreted and transmembrane proteins expressed during embryogenesis.

(Kopczynski et al., 1998)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 54 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39410

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

M9PI37

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

M9PI37

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0036279

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39410/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036279

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036279

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036279

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4357

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0036279

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39410

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4357

MARRVEL_MODEL - MARRVEL (model organism gene)

39410

modMine - A data warehouse for the modENCODE project

FBgn0036279

Linkouts

BioGRID - A database of protein and genetic interactions.

64762

DroID - A comprehensive database of gene and protein interactions.

FBgn0036279

DRSC - Results frm RNAi screens

FBgn0036279

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0036279

FlyMine - An integrated database for Drosophila genomics

FBgn0036279

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Cation-coupled Chloride cotransporters

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

BEST:CK01027

CG43567

(Filippov et al., 2002)

CG4357

(Sun et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Romero-Calderon et al., 2007, Inlow and Restifo, 2004, Rana, 2004.7.8, Wang et al., 2004, Filippov et al., 2003)

CK01027

(Kopczynski et al., 1998)

NCC69

(Leiserson and Keshishian, 2005)

NKCC

(Sun et al., 2018)

Ncc69

(Scepanovic et al., 2021, Rodan, 2019, Stenesen et al., 2019, Yildirim et al., 2019, Rajpurohit et al., 2018, Ng et al., 2016, Parinejad et al., 2016, Ashwal-Fluss et al., 2014, Chen et al., 2014, Limmer et al., 2014, Wu et al., 2014, Rodan et al., 2012, Wilczynski et al., 2012, Featherstone, 2011, Leiserson and Keshishian, 2011, Leiserson et al., 2011, Hekmat-Scafe et al., 2010, Sun et al., 2010)

mdcds_25302

(Chen and Oliver, 2014.8.15)

ncc69

(Hope et al., 2020)

Name Synonyms

sodium chloride cotransporter 69

(Mukherjee et al., 2020, Leiserson, 2005.3.2)

Secondary FlyBase IDs