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Gene Dmel\Ncc69

General Information
Symbol	Dmel\Ncc69	Species	D. melanogaster
Name	sodium chloride cotransporter 69	Annotation symbol	CG4357
Feature type	protein_coding_gene	FlyBase ID	FBgn0036279
Gene Model Status	Current	Stock availability	8 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	69B3-69B3	Sequence location	3L:12,361,746..12,384,539 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene sodium chloride cotransporter 69 is referred to in FlyBase by the symbol Dmel\Ncc69 (CG4357, FBgn0036279). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sodium:potassium:chloride symporter activity. Based on sequence similarity, it is predicted to be involved in the biological process: amino acid transmembrane transport. 11 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Amino acid permease domain; Na/K/Cl co-transporter; Na/K/Cl co-transporter superfamily. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 and 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult eye. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1626461_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Ncc69. Gene sequence location is 3L:12361746..12384539.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN28763 HFA10413 HFA10412 Controlled Vocabulary Terms adult thorax faint female organism embryonic stage male adult abdomen adult stage adult midgut adult head
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 69B3-69B3   Limits computationally determined from genome sequence between P{PZ}l(3)0508805088 and P{PZ}l(3)0692406924
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Ncc69 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Ncc69-RA FBtr0075987  NM_140315   3931   1171 Ncc69-RB FBtr0075988  NM_206344   3994   1171
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Ncc69-PA   FBpp0075719   129.2   1171   6.82     NP_648572   AAF49927 Ncc69-PB   FBpp0075720   129.2   1171   6.82     NP_996066   AAS65014
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Na/K/Cl co-transporter (IPR002443) Amino acid permease domain (IPR004841) Na/K/Cl co-transporter superfamily (IPR004842)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA140937   AE014296 AAF49927   AE014296 AAS65014   AI108446   AI258782   AI260249   AI293519   AI402994   AI404076   AI513989   AI517579   AI517815   AI542698   AW941039   AY094735 AAM11088   BG632370   BG633288   BI236016   BI241466   BI356547   BI613297   BT001426   CK657219   CK659386   CO277447   CO278060   CO286613   CO297450   CO329103   EC087364   EC206993   EC221474   EC229961   EC243675
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VTW8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
RT-PCR Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | faint   (Filippov et al., 2003) adult stage   adult midgut | faint   (Filippov et al., 2003) adult head | faint   (Filippov et al., 2003) adult stage | male   adult thorax | faint & adult abdomen | faint   (Filippov et al., 2003) Comment:reference stages "body" adult stage | female   adult thorax | faint & adult abdomen | faint   (Filippov et al., 2003) Comment:reference stages "body"
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0036279    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0036279 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 and 18-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0036279 expression level Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    99 embryo 04-06hr    213 embryo 06-08hr    1644 embryo 08-10hr    2673 embryo 10-12hr    4345 embryo 12-14hr    3677 embryo 14-16hr    2419 embryo 16-18hr    3264 embryo 18-20hr    4715 embryo 20-22hr    2694 embryo 22-24hr    2878 larva L1    2544 larva L2    1932 larva L3 12hr old    1500 larva L3 puffstage 1-2    1801 larva L3 puffstage 3-6    2467 larva L3 puffstage 7-9    2983 white prepupae new    3239 white prepupae 12hr    2409 white prepupae 24hr    2040 pupae 2d postWPP    1983 pupae 3d postWPP    1454 pupae 4d postWPP    2844 adult male 01day    2657 adult male 05day    2720 adult male 30day    3070 adult female 01day    2156 adult female 05day    1546 adult female 30day    1372 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    99 embryo 04-06hr  (213) embryo 06-08hr  (1644) embryo 08-10hr  (2673) embryo 10-12hr  (4345) embryo 12-14hr  (3677) embryo 14-16hr  (2419) embryo 16-18hr  (3264) embryo 18-20hr  (4715) embryo 20-22hr  (2694) embryo 22-24hr  (2878) larva L1  (2544) larva L2  (1932) larva L3 12hr old  (1500) larva L3 puffstage 1-2  (1801) larva L3 puffstage 3-6  (2467) larva L3 puffstage 7-9  (2983) white prepupae new  (3239) white prepupae 12hr  (2409) white prepupae 24hr  (2040) pupae 2d postWPP  (1983) pupae 3d postWPP  (1454) pupae 4d postWPP  (2844) adult male 01day  (2657) adult male 05day  (2720) adult male 30day  (3070) adult female 01day  (2156) adult female 05day  (1546) adult female 30day  (1372) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    99 embryo 04-06hr    213 embryo 06-08hr  (1644) embryo 08-10hr  (2673) embryo 10-12hr  (4345) embryo 12-14hr  (3677) embryo 14-16hr  (2419) embryo 16-18hr  (3264) embryo 18-20hr  (4715) embryo 20-22hr  (2694) embryo 22-24hr  (2878) larva L1  (2544) larva L2  (1932) larva L3 12hr old  1500 larva L3 puffstage 1-2  (1801) larva L3 puffstage 3-6  (2467) larva L3 puffstage 7-9  (2983) white prepupae new  (3239) white prepupae 12hr  (2409) white prepupae 24hr  (2040) pupae 2d postWPP  (1983) pupae 3d postWPP  1454 pupae 4d postWPP  (2844) adult male 01day  (2657) adult male 05day  (2720) adult male 30day  (3070) adult female 01day  (2156) adult female 05day  (1546) adult female 30day    1372 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    99 embryo 04-06hr    213 embryo 06-08hr    1644 embryo 08-10hr    2673 embryo 10-12hr    4345 embryo 12-14hr    3677 embryo 14-16hr    2419 embryo 16-18hr    3264 embryo 18-20hr    4715 embryo 20-22hr    2694 embryo 22-24hr    2878 larva L1    2544 larva L2    1932 larva L3 12hr old    1500 larva L3 puffstage 1-2    1801 larva L3 puffstage 3-6    2467 larva L3 puffstage 7-9    2983 white prepupae new    3239 white prepupae 12hr    2409 white prepupae 24hr    2040 pupae 2d postWPP    1983 pupae 3d postWPP    1454 pupae 4d postWPP    2844 adult male 01day    2657 adult male 05day    2720 adult male 30day    3070 adult female 01day    2156 adult female 05day    1546 adult female 30day    1372 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    99 embryo 04-06hr    213 embryo 06-08hr    1644 embryo 08-10hr    2673 embryo 10-12hr    4345 embryo 12-14hr    3677 embryo 14-16hr    2419 embryo 16-18hr    3264 embryo 18-20hr    4715 embryo 20-22hr    2694 embryo 22-24hr    2878 larva L1    2544 larva L2    1932 larva L3 12hr old    1500 larva L3 puffstage 1-2    1801 larva L3 puffstage 3-6    2467 larva L3 puffstage 7-9    2983 white prepupae new    3239 white prepupae 12hr    2409 white prepupae 24hr    2040 pupae 2d postWPP    1983 pupae 3d postWPP    1454 pupae 4d postWPP    2844 adult male 01day    2657 adult male 05day    2720 adult male 30day    3070 adult female 01day    2156 adult female 05day    1546 adult female 30day    1372 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0036279 expression level Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    99 embryo 04-06hr    213 embryo 06-08hr    1644 embryo 08-10hr    2673 embryo 10-12hr    4345 embryo 12-14hr    3677 embryo 14-16hr    2419 embryo 16-18hr    3264 embryo 18-20hr    4715 embryo 20-22hr    2694 embryo 22-24hr    2878 larva L1    2544 larva L2    1932 larva L3 12hr old    1500 larva L3 puffstage 1-2    1801 larva L3 puffstage 3-6    2467 larva L3 puffstage 7-9    2983 white prepupae new    3239 white prepupae 12hr    2409 white prepupae 24hr    2040 pupae 2d postWPP    1983 pupae 3d postWPP    1454 pupae 4d postWPP    2844 adult male 01day    2657 adult male 05day    2720 adult male 30day    3070 adult female 01day    2156 adult female 05day    1546 adult female 30day    1372 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    99 embryo 04-06hr  (213) embryo 06-08hr  (1644) embryo 08-10hr  (2673) embryo 10-12hr  (4345) embryo 12-14hr  (3677) embryo 14-16hr  (2419) embryo 16-18hr  (3264) embryo 18-20hr  (4715) embryo 20-22hr  (2694) embryo 22-24hr  (2878) larva L1  (2544) larva L2  (1932) larva L3 12hr old  (1500) larva L3 puffstage 1-2  (1801) larva L3 puffstage 3-6  (2467) larva L3 puffstage 7-9  (2983) white prepupae new  (3239) white prepupae 12hr  (2409) white prepupae 24hr  (2040) pupae 2d postWPP  (1983) pupae 3d postWPP  (1454) pupae 4d postWPP  (2844) adult male 01day  (2657) adult male 05day  (2720) adult male 30day  (3070) adult female 01day  (2156) adult female 05day  (1546) adult female 30day  (1372) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    99 embryo 04-06hr    213 embryo 06-08hr  1644 embryo 08-10hr  (2673) embryo 10-12hr  (4345) embryo 12-14hr  (3677) embryo 14-16hr  2419 embryo 16-18hr  (3264) embryo 18-20hr  (4715) embryo 20-22hr  (2694) embryo 22-24hr  (2878) larva L1  (2544) larva L2  1932 larva L3 12hr old  1500 larva L3 puffstage 1-2  1801 larva L3 puffstage 3-6  2467 larva L3 puffstage 7-9  (2983) white prepupae new  (3239) white prepupae 12hr  2409 white prepupae 24hr  2040 pupae 2d postWPP  1983 pupae 3d postWPP  1454 pupae 4d postWPP  (2844) adult male 01day  (2657) adult male 05day  (2720) adult male 30day  (3070) adult female 01day  2156 adult female 05day  1546 adult female 30day    1372 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    99 embryo 04-06hr    213 embryo 06-08hr    1644 embryo 08-10hr    2673 embryo 10-12hr    4345 embryo 12-14hr    3677 embryo 14-16hr    2419 embryo 16-18hr    3264 embryo 18-20hr    4715 embryo 20-22hr    2694 embryo 22-24hr    2878 larva L1    2544 larva L2    1932 larva L3 12hr old    1500 larva L3 puffstage 1-2    1801 larva L3 puffstage 3-6    2467 larva L3 puffstage 7-9    2983 white prepupae new    3239 white prepupae 12hr    2409 white prepupae 24hr    2040 pupae 2d postWPP    1983 pupae 3d postWPP    1454 pupae 4d postWPP    2844 adult male 01day    2657 adult male 05day    2720 adult male 30day    3070 adult female 01day    2156 adult female 05day    1546 adult female 30day    1372 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    99 embryo 04-06hr    213 embryo 06-08hr    1644 embryo 08-10hr    2673 embryo 10-12hr    4345 embryo 12-14hr    3677 embryo 14-16hr    2419 embryo 16-18hr    3264 embryo 18-20hr    4715 embryo 20-22hr    2694 embryo 22-24hr    2878 larva L1    2544 larva L2    1932 larva L3 12hr old    1500 larva L3 puffstage 1-2    1801 larva L3 puffstage 3-6    2467 larva L3 puffstage 7-9    2983 white prepupae new    3239 white prepupae 12hr    2409 white prepupae 24hr    2040 pupae 2d postWPP    1983 pupae 3d postWPP    1454 pupae 4d postWPP    2844 adult male 01day    2657 adult male 05day    2720 adult male 30day    3070 adult female 01day    2156 adult female 05day    1546 adult female 30day    1372 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0036279    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0036279 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult eye. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0036279 expression level Tissue   Expression Level Larval Central Nervous System    102.475 Larval Midgut    87.3 Larval Hindgut    74.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    174.7 Larval Trachea    137.2 Larval Carcass    163.225 Adult Head    197.3 Adult Eye    734.25 Adult Brain    159.7 Adult Thoracic-Abdominal Ganglion    461.2 Adult Crop    207.2 Adult Midgut    61.8 Adult Hindgut    29.4 Adult Malpighian Tubules    7.9 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    40.85 Adult VirginFemale Spermatheca    169 Adult InseminatedFemale Spermatheca    136.3 Adult Ovary    78.3 Adult Testis    97.3 Adult Male Accessory Gland    260.4 Adult Carcass    127 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    102.475 Larval Midgut    87.3 Larval Hindgut    74.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    174.7 Larval Trachea    137.2 Larval Carcass    163.225 Adult Head    197.3 Adult Eye  (734.25) Adult Brain    159.7 Adult Thoracic-Abdominal Ganglion    461.2 Adult Crop    207.2 Adult Midgut    61.8 Adult Hindgut    29.4 Adult Malpighian Tubules    7.9 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    40.85 Adult VirginFemale Spermatheca    169 Adult InseminatedFemale Spermatheca    136.3 Adult Ovary    78.3 Adult Testis    97.3 Adult Male Accessory Gland    260.4 Adult Carcass    127 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    102.475 Larval Midgut    87.3 Larval Hindgut    74.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    174.7 Larval Trachea    137.2 Larval Carcass    163.225 Adult Head    197.3 Adult Eye    734.25 Adult Brain    159.7 Adult Thoracic-Abdominal Ganglion    461.2 Adult Crop    207.2 Adult Midgut    61.8 Adult Hindgut    29.4 Adult Malpighian Tubules    7.9 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    40.85 Adult VirginFemale Spermatheca    169 Adult InseminatedFemale Spermatheca    136.3 Adult Ovary    78.3 Adult Testis    97.3 Adult Male Accessory Gland    260.4 Adult Carcass    127 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    102.475 Larval Midgut    87.3 Larval Hindgut    74.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    174.7 Larval Trachea    137.2 Larval Carcass    163.225 Adult Head    197.3 Adult Eye    734.25 Adult Brain    159.7 Adult Thoracic-Abdominal Ganglion    461.2 Adult Crop    207.2 Adult Midgut    61.8 Adult Hindgut    29.4 Adult Malpighian Tubules    7.9 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    40.85 Adult VirginFemale Spermatheca    169 Adult InseminatedFemale Spermatheca    136.3 Adult Ovary    78.3 Adult Testis    97.3 Adult Male Accessory Gland    260.4 Adult Carcass    127 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0036279 expression level Tissue   Expression Level Larval Central Nervous System    102.475 Larval Midgut    87.3 Larval Hindgut    74.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    174.7 Larval Trachea    137.2 Larval Carcass    163.225 Adult Head    197.3 Adult Eye    734.25 Adult Brain    159.7 Adult Thoracic-Abdominal Ganglion    461.2 Adult Crop    207.2 Adult Midgut    61.8 Adult Hindgut    29.4 Adult Malpighian Tubules    7.9 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    40.85 Adult VirginFemale Spermatheca    169 Adult InseminatedFemale Spermatheca    136.3 Adult Ovary    78.3 Adult Testis    97.3 Adult Male Accessory Gland    260.4 Adult Carcass    127 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    102.475 Larval Midgut    87.3 Larval Hindgut    74.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    174.7 Larval Trachea    137.2 Larval Carcass    163.225 Adult Head    197.3 Adult Eye  734.25 Adult Brain    159.7 Adult Thoracic-Abdominal Ganglion    461.2 Adult Crop    207.2 Adult Midgut    61.8 Adult Hindgut    29.4 Adult Malpighian Tubules    7.9 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    40.85 Adult VirginFemale Spermatheca    169 Adult InseminatedFemale Spermatheca    136.3 Adult Ovary    78.3 Adult Testis    97.3 Adult Male Accessory Gland    260.4 Adult Carcass    127 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    102.475 Larval Midgut    87.3 Larval Hindgut    74.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    174.7 Larval Trachea    137.2 Larval Carcass    163.225 Adult Head    197.3 Adult Eye    734.25 Adult Brain    159.7 Adult Thoracic-Abdominal Ganglion    461.2 Adult Crop    207.2 Adult Midgut    61.8 Adult Hindgut    29.4 Adult Malpighian Tubules    7.9 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    40.85 Adult VirginFemale Spermatheca    169 Adult InseminatedFemale Spermatheca    136.3 Adult Ovary    78.3 Adult Testis    97.3 Adult Male Accessory Gland    260.4 Adult Carcass    127 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    102.475 Larval Midgut    87.3 Larval Hindgut    74.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    174.7 Larval Trachea    137.2 Larval Carcass    163.225 Adult Head    197.3 Adult Eye    734.25 Adult Brain    159.7 Adult Thoracic-Abdominal Ganglion    461.2 Adult Crop    207.2 Adult Midgut    61.8 Adult Hindgut    29.4 Adult Malpighian Tubules    7.9 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    40.85 Adult VirginFemale Spermatheca    169 Adult InseminatedFemale Spermatheca    136.3 Adult Ovary    78.3 Adult Testis    97.3 Adult Male Accessory Gland    260.4 Adult Carcass    127 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    197.3   NA  Eye    734.25   NA  Brain    159.7   102.475  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    461.2   NA  Crop    207.2   87.3  Midgut    61.8   74.4  Hindgut    29.4   no informative data  Malpighian Tubules    7.9   no informative data  Fat Body    no informative data   174.7  Salivary Gland    no informative data   NA  Heart    40.85   137.2  Trachea    NA   NA  VirginFemale Spermatheca    169   NA  InseminatedFemale Spermatheca    136.3   NA  Ovary    78.3   NA  Testis    97.3   NA  Male Accessory Gland    260.4   163.225  Carcass    127 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_09
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0036279 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4357-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Ncc69PL00618 Ncc69r1 Ncc69r2 viable, with Scer\GAL4pnr-MD237 Ncc69GD14863 Sterility Allele fertile Ncc69PL00618 Other Phenotypes Allele neuroanatomy defective | third instar larval stage Ncc69r1 Ncc69r2 neuroanatomy defective | third instar larval stage, with Scer\GAL4Gli-rL82.29 Ncc69GD14863 Phenotype manifest in Allele abdominal nerve | larval stage Ncc69r1 abdominal nerve | third instar larval stage Ncc69r2 abdominal nerve | third instar larval stage, with Scer\GAL4Gli-rL82.29 Ncc69GD14863
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of Ncc69 Class Mutagen Stocks Known lesion Ncc69DG04311 P-element activity 1 -- Ncc69MB05292 1 -- Ncc69MI00828 1 -- Ncc69PL00618 P-element activity 1 -- Ncc69r1 amorphic allele - genetic evidence P-element activity 0 Yes Ncc69r2 amorphic allele - genetic evidence P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Ncc69 Class Mutagen Stocks Known lesion Ncc69GD14863 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Ncc69JF03097 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ncc69KK108763 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ncc69GD2440 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Ncc69Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD2440} NA P{GD14863} NA P{KK108763} NA P{TRiP.JF03097} NA P{UAS-HA-Ncc69} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap binary system Mi{ET1}Ncc69MB05292 No PBac{GAL4D,EYFP}Ncc69PL00618 No miscellaneous insertions Mi{MIC}Ncc69MI00828 NA P{wHy}Ncc69DG04311 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function
CV term Evidence References
sodium:potassium:chloride symporter activity inferred from direct assay (Leiserson et al., 2011)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
amino acid transmembrane transporter activity inferred from sequence or structural similarity with Eaat1 (Romero-Calderon et al., 2007) sodium:chloride symporter activity inferred from sequence or structural similarity with UniProtKB:Q25479 (FlyBase, 1992-)
Biological Process
CV term Evidence References
amino acid transmembrane transport inferred from sequence or structural similarity with Eaat1 (Romero-Calderon et al., 2007) chloride transport inferred from electronic annotation with InterPro:IPR004842 (FlyBase Curators et al., 2004-) sodium ion transport inferred from electronic annotation with InterPro:IPR004842 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR004842 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Ncc69 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64762 DroID - A comprehensive database of gene and protein interactions. • FBgn0036279 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39410
Orthologs
Genome-wide drosophilid orthologs	Dana\GF24985 Dere\GG13837 Dgri\GH16072 Dmoj\GI12335 Dper\GL24962 Dpse\GA18131 Dsec\GM24663 Dsim\GD12726 Dvir\GJ12226 Dwil\GK12754 Dyak\GE20127
Curated drosophilid orthologs	Dpse\GA18131
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL006180-PA Aedes aegypti (mosquito) AGAP001557-PA Anopheles gambiae (mosquito) XP_001943581.1 Acyrthosiphon pisum (pea aphid) 78583 Branchiostoma floridae (lancelet) BGIBMGA012368-PA Bombyx mori (silk worm) ENSBTAP00000012701 Bos taurus (bovine) CN21494 Caenorhabditis brenneri (small nematode) CBP26993 Caenorhabditis briggsae (nematode) CE40530 Caenorhabditis elegans (worm) ENSCAFP00000000906 Canis familiaris (dog) ENSCINP00000017039 Ciona intestinalis (sea squirt) CPIJ019752 Culex pipens (mosquito) ENSCPOP00000013092 Cavia porcellus (guinea pig) RP33416 Caenorhabditis remanei (nematode) 156991 Capitella spl (polychaete worm) 324617 Daphnia pulex (daphnia) ENSDARP00000025500 Danio rerio (zebra fish) ENSECAP00000013091 Equus caballus (horse) ENSGACP00000024256 Gymnopilus aculeatus (mushroom) ENSGALP00000036922 Gallus gallus (chicken) 116093 Hypsipyla robusta (cedar shoot borer) ENSP00000262461 Homo sapiens (human) ISCW001819-PA Ixodes scapularis (deer tick) 177989 Lottia gigantea (owl limpet) ENSMODP00000003303 Monodelphis domestica (gray opossum) ENSMMUP00000014701 Macaca mulatta (rhesus monkey) ENSMUSP00000111023 Mus musculus (house mouse) 104399 Nematostella vectensis (starlet anemone) ENSOANP00000021692 Ornithorynchus anatinus (platypus) ENSORLP00000021358 Oryzias latipes (japanese killifish) PHUM000651-PA Pediculus humanus (human body louse) PP27939 Pristionchus pacificus (nematode) ENSPPYP00000017600 Pongo pygmaeus (orangutan) ENSPTRP00000029417 Pan troglodytes (chimpanzee) ENSRNOP00000021921 Rattus norvegicus (norway rat) XP_783014.2 Strongylocentrotus purpuratus (purple sea urchin) 22993 Trichoplax adhaerens (metazoa) XP_969047.2 Tribolium castaneum (red flour beetle) ENSTNIP00000019852 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000004450 Takifugu rubripes (pufferfish Takifugu) 87848.m00050 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000011203 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13033 NV50006 LH14510 LH21364 ISCW001819 FBgn0242357 FBgn0237450 FBgn0228952 FBgn0216029 FBgn0214763 FBgn0211641 FBgn0199476 FBgn0191105 FBgn0184453 FBgn0179526 FBgn0171469 FBgn0165574 JGI_V11_203293 JGI_V11_324595 JGI_V11_307471 JGI_V11_324602 JGI_V11_324617 JGI_V11_319556 FBgn0078139 FBgn0076332 FBgn0162549 FBgn0159753 FBgn0147217 FBgn0135092 FBgn0051547 FBgn0126461 FBgn0123543 FBgn0106100 FBgn0103289 FBgn0101977 FBgn0094723 CPIJ019752 CPIJ007505 BGIBMGA012368 BGIBMGA001472 ACYPI001649 GB18271 GB17120 GB15653 AGAP003275 AGAP001557 AAEL009888 AAEL006180 PB19922 PHUM399390 PHUM399960 PHUM417670 GLEAN_00573 GLEAN_15462
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	24611 w1118; Mi{ET1}Ncc69MB05292 28682 y1 v1; P{TRiP.JF03097}attP2 33088 y1 w*; Mi{MIC}Ncc69MI00828 20377 y1 w67c23; P{wHy}Ncc69DG04311 19568 w*; PBac{GAL4D,EYFP}Ncc69PL00618 P{FRT(whs)}2A P{neoFRT}82B
VDRC	v106499 P{KK108763}VIE-260B v30000 w1118; P{GD14863}v30000 v30001 w1118; P{GD14863}v30001
Genomic Clones ( 1 )
	BACR26C20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 28 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH15356 GH27027
Other clones	DPiM_GH15356
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH22346 GH28585 GH28878 LP02060 LP03971 LP06683 LP17117 LP23337 RE32002 RE38545 RE42724 RH42420 SD09140
Other clones	5353 16982 83304 CK01.60A.E6 CK01027 EK063351 EK124453 EK128631 EK217015 EK292033 GH07280 GH23666
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0036279 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39410
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Ncc69 CG4357 (Leiserson, 2005.3.2)
Source for database merge of	Source for merge of: CG4357 BEST:CK01027 (Rana, 2004.7.8)
Additional comments
Other Comments
	Identification: as a protein that physically interacts with the N-terminal region of the fray gene product. (Leiserson and Keshishian, 2005) Identification: cDNA screen for secreted and transmembrane proteins expressed during embryogenesis. (Kopczynski et al., 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140315 NM_206344 RefSeq (Proteins) • NP_648572 NP_996066 DDBJ / EMBL / GenBank • AA140937 AAF49927 AAM11088 AAS65014 AI513989 AW941039 AY094735 Entrez Gene - A searchable database of RefSeq genes. • 39410 UniProtKB/TrEMBL • Q9VTW8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Na/K/Cl co-transporter (IPR002443) Amino acid permease domain (IPR004841) Na/K/Cl co-transporter superfamily (IPR004842)
Linkouts
	BioGRID - A database of protein and genetic interactions • 64762 DroID - A comprehensive database of gene and protein interactions. • FBgn0036279 DRSC - Results from RNAi screens. • FBgn0036279 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0036279 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4357-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0036279 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39410 InParanoid A subset of ortholog calls from InParanoid. • AAEL006180-PA Aedes aegypti (mosquito) AGAP001557-PA Anopheles gambiae (mosquito) XP_001943581.1 Acyrthosiphon pisum (pea aphid) 78583 Branchiostoma floridae (lancelet) BGIBMGA012368-PA Bombyx mori (silk worm) ENSBTAP00000012701 Bos taurus (bovine) CN21494 Caenorhabditis brenneri (small nematode) CBP26993 Caenorhabditis briggsae (nematode) CE40530 Caenorhabditis elegans (worm) ENSCAFP00000000906 Canis familiaris (dog) ENSCINP00000017039 Ciona intestinalis (sea squirt) CPIJ019752 Culex pipens (mosquito) ENSCPOP00000013092 Cavia porcellus (guinea pig) RP33416 Caenorhabditis remanei (nematode) 156991 Capitella spl (polychaete worm) 324617 Daphnia pulex (daphnia) ENSDARP00000025500 Danio rerio (zebra fish) ENSECAP00000013091 Equus caballus (horse) ENSGACP00000024256 Gymnopilus aculeatus (mushroom) ENSGALP00000036922 Gallus gallus (chicken) 116093 Hypsipyla robusta (cedar shoot borer) ENSP00000262461 Homo sapiens (human) ISCW001819-PA Ixodes scapularis (deer tick) 177989 Lottia gigantea (owl limpet) ENSMODP00000003303 Monodelphis domestica (gray opossum) ENSMMUP00000014701 Macaca mulatta (rhesus monkey) ENSMUSP00000111023 Mus musculus (house mouse) 104399 Nematostella vectensis (starlet anemone) ENSOANP00000021692 Ornithorynchus anatinus (platypus) ENSORLP00000021358 Oryzias latipes (japanese killifish) PHUM000651-PA Pediculus humanus (human body louse) PP27939 Pristionchus pacificus (nematode) ENSPPYP00000017600 Pongo pygmaeus (orangutan) ENSPTRP00000029417 Pan troglodytes (chimpanzee) ENSRNOP00000021921 Rattus norvegicus (norway rat) XP_783014.2 Strongylocentrotus purpuratus (purple sea urchin) 22993 Trichoplax adhaerens (metazoa) XP_969047.2 Tribolium castaneum (red flour beetle) ENSTNIP00000019852 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000004450 Takifugu rubripes (pufferfish Takifugu) 87848.m00050 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000011203 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39410 modMine - Data generated by the modENCODE project. • FBgn0036279 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13033 NV50006 LH14510 LH21364 ISCW001819 FBgn0242357 FBgn0237450 FBgn0228952 FBgn0216029 FBgn0214763 FBgn0211641 FBgn0199476 FBgn0191105 FBgn0184453 FBgn0179526 FBgn0171469 FBgn0165574 JGI_V11_203293 JGI_V11_324595 JGI_V11_307471 JGI_V11_324602 JGI_V11_324617 JGI_V11_319556 FBgn0078139 FBgn0076332 FBgn0162549 FBgn0159753 FBgn0147217 FBgn0135092 FBgn0051547 FBgn0126461 FBgn0123543 FBgn0106100 FBgn0103289 FBgn0101977 FBgn0094723 CPIJ019752 CPIJ007505 BGIBMGA012368 BGIBMGA001472 ACYPI001649 GB18271 GB17120 GB15653 AGAP003275 AGAP001557 AAEL009888 AAEL006180 PB19922 PHUM399390 PHUM399960 PHUM417670 GLEAN_00573 GLEAN_15462
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	BEST:CK01027   CG4357 (Inlow and Restifo, 2004, Rana, 2004.7.8, Romero-Calderon et al., 2007, Wang et al., 2004, Filippov et al., 2003, Keleman et al., 2009.8.5, Perkins et al., 2009) CG43567 (Filippov et al., 2002) CK01027 (Kopczynski et al., 1998) NCC69 (Leiserson and Keshishian, 2005) Ncc69 (Hekmat-Scafe et al., 2010, Leiserson et al., 2011)
Name Synonym	sodium chloride cotransporter 69 (Leiserson, 2005.3.2)
Secondary FlyBase IDs
	FBgn0025706
References ( 29 )
Generate a list of	All references relating to this gene ( 29 )
List References by type	Research paper  ( 7 ) Supplementary material  ( 3 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 2 ) FlyBase analysis  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 2 )
	Leiserson et al., 2011, Glia 59(2): 320--332 Drosophila glia use a conserved cotransporter mechanism to regulate extracellular volume. [FBrf0212638] Hekmat-Scafe et al., 2010, Genetics 184(1): 171--183 Seizure sensitivity is ameliorated by targeted expression of k+-cl- cotransporter function in the mushroom body of the Drosophila brain. [FBrf0209683] Show all research papers ...