FB2020_06, released Dec 22, 2020

Gene: Dmel\Ncc69

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General Information
Symbol
Dmel\Ncc69
Species
D. melanogaster
Name
sodium chloride cotransporter 69
Annotation Symbol
CG4357
Feature Type
protein_coding_gene
FlyBase ID
FBgn0036279
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Snapshot
sodium chloride cotransporter 69 (Ncc69) encodes a member of the SLC12 family of Na[+]/K[+]/2Cl[-] cotransporters (NKCC). [Date last reviewed: 2019-03-14]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
69B3-69B3
Sequence location
3L:12,368,646..12,391,444 [-]
Recombination map

3-38

RefSeq locus
NT_037436 REGION:12368646..12391444
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (15 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables sodium:potassium:chloride symporter activity
inferred from direct assay
(Leiserson et al., 2011, Sun et al., 2010)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables amino acid transmembrane transporter activity
inferred from sequence or structural similarity with FLYBASE:Eaat1; FB:FBgn0026439
(Romero-Calderon et al., 2007)
enables potassium:chloride symporter activity
inferred from biological aspect of ancestor with PANTHER:PTN000214719
(assigned by GO_Central )
(Gaudet et al., 2011)
enables sodium:potassium:chloride symporter activity
inferred from biological aspect of ancestor with PANTHER:PTN000930537
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (10 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in chloride transport
inferred from direct assay
(Sun et al., 2010)
involved_in potassium ion transport
inferred from mutant phenotype
(Wu et al., 2014)
inferred from direct assay
(Sun et al., 2010)
Terms Based on Predictions or Assertions (8 terms)
CV Term
Evidence
References
involved_in amino acid transmembrane transport
inferred from sequence or structural similarity with FLYBASE:Eaat1; FB:FBgn0026439
(Romero-Calderon et al., 2007)
involved_in cell volume homeostasis
inferred from biological aspect of ancestor with PANTHER:PTN000214719
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in chloride ion homeostasis
inferred from biological aspect of ancestor with PANTHER:PTN000214719
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in chloride transmembrane transport
inferred from biological aspect of ancestor with PANTHER:PTN000214719
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in potassium ion homeostasis
inferred from biological aspect of ancestor with PANTHER:PTN000214719
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in potassium ion import across plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000214719
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in sodium ion homeostasis
inferred from biological aspect of ancestor with PANTHER:PTN000930537
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in sodium ion transmembrane transport
inferred from biological aspect of ancestor with PANTHER:PTN000930537
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in plasma membrane
inferred from direct assay
(Sun et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in integral component of membrane
inferred from electronic annotation with InterPro:IPR004842
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
SLC12 FAMILY OF CATION-COUPLED CHLORIDE TRANSPORTERS -
Solute carrier family 12 (SLC12) members are sodium and/or potassium-coupled chloride transporters. (Adapted from FBrf0209627).
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\Ncc69 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Gene model reviewed during 6.02

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Sequence Ontology: Class of Gene
gene_with_edited_transcript
(Graveley et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Ncc69-RA
FBtr0075987
NM_140315
3936
1171
Ncc69-RC
FBtr0333838
NM_001274827
4822
1191
Ncc69-RD
FBtr0333839
NM_001274828
3996
1191
Ncc69-RE
FBtr0333840
NM_001274829
4044
1207
Ncc69-RF
FBtr0333841
NM_001274830
4051
1191
Ncc69-RG
FBtr0333842
NM_206344
4762
1171
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Ncc69-PA
FBpp0075719
129.2
1171
6.82
NP_648572
AAF49927
Ncc69-PC
FBpp0305972
131.6
1191
6.99
NP_001261756
AGB94449
Ncc69-PD
FBpp0305973
131.6
1191
6.99
NP_001261757
AGB94450
Ncc69-PE
FBpp0305974
133.5
1207
7.77
NP_001261758
AGB94451
Ncc69-PF
FBpp0305975
131.6
1191
6.99
NP_001261759
AGB94452
Ncc69-PG
FBpp0305976
129.2
1171
6.82
NP_996066
AAS65014
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1171 aa isoforms: Ncc69-PA, Ncc69-PG
1191 aa isoforms: Ncc69-PC, Ncc69-PD, Ncc69-PF
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ncc69 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 6
organism
(Sun et al., 2010)
embryonic stage 9 -- 13
embryonic/larval midgut primordium
(Sun et al., 2010)
presumptive embryonic/larval central nervous system
(Sun et al., 2010)
embryonic stage 14 -- 17
embryonic/larval foregut
(Sun et al., 2010)
embryonic stage 16
embryonic/larval CNS subperineurial glial cell
(Leiserson et al., 2011)
dorsal median cell
(Leiserson et al., 2011)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Romero-Calderon et al., 2007)
adult thorax | faint

Comment: Thorax and abdomen assayed together.

(Romero-Calderon et al., 2007)
adult abdomen | faint

Comment: Thorax and abdomen assayed together.

(Romero-Calderon et al., 2007)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
organism | faint
(Filippov et al., 2003)
adult stage
adult midgut | faint
(Filippov et al., 2003)
adult head | faint
(Filippov et al., 2003)
adult stage | male
adult thorax | faint & adult abdomen | faint

Comment: reference stages "body"

(Filippov et al., 2003)
adult stage | female
adult thorax | faint & adult abdomen | faint

Comment: reference stages "body"

(Filippov et al., 2003)
Additional Descriptive Data

Ncc69 is expressed in subperineurial glial cells. The only other prominent expression of Ncc69 is in the dorsal median cells of the ventral ganglion.

(Leiserson et al., 2011)

During embyronic stages 9-13, expression is seen in the developing gut and nervous system. In later stages, expression is predominantly in the foregut and anterior portion of the embryo.

(Sun et al., 2010)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult lamina satellite glial cell
(Stenesen et al., 2019)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

Ncc69 protein is expressed in glial cells. In the adult eye, Ncc69 protein expression is enriched in satellite glial cells in the distal lamina.

(Stenesen et al., 2019)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in plasma membrane
inferred from direct assay
(Sun et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ncc69 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 7 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Ncc69
Transgenic constructs containing regulatory region of Ncc69
Deletions and Duplications ( 1 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (22)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\SLC12A2
15 of 15
Yes
Yes
1  
Hsap\SLC12A1
13 of 15
No
Yes
Hsap\SLC12A3
12 of 15
No
Yes
Hsap\SLC12A4
2 of 15
No
No
1  
Hsap\SLC12A5
2 of 15
No
No
2  
Hsap\SLC12A6
2 of 15
No
No
Hsap\SLC12A7
2 of 15
No
No
Hsap\SLC7A1
1 of 15
No
No
Hsap\SLC7A2
1 of 15
No
No
Hsap\SLC7A3
1 of 15
No
No
Hsap\SLC7A4
1 of 15
No
No
Hsap\SLC7A5
1 of 15
No
No
Hsap\SLC7A6
1 of 15
No
No
Hsap\SLC7A7
1 of 15
No
No
Hsap\SLC7A8
1 of 15
No
No
Hsap\SLC7A9
1 of 15
No
No
Hsap\SLC7A10
1 of 15
No
No
Hsap\SLC7A11
1 of 15
No
No
Hsap\SLC7A13
1 of 15
No
No
Hsap\SLC7A14
1 of 15
No
No
Hsap\SLC12A8
1 of 15
No
No
Hsap\SLC12A9
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (25)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Slc12a2
15 of 15
Yes
Yes
Mmus\Slc12a1
12 of 15
No
Yes
Mmus\Slc12a3
12 of 15
No
Yes
Mmus\Slc12a4
2 of 15
No
No
Mmus\Slc12a5
2 of 15
No
No
Mmus\Slc12a6
2 of 15
No
No
Mmus\Slc12a7
2 of 15
No
No
Mmus\AU018091
1 of 15
No
No
Mmus\Slc7a1
1 of 15
No
No
Mmus\Slc7a2
1 of 15
No
No
Mmus\Slc7a3
1 of 15
No
No
Mmus\Slc7a4
1 of 15
No
No
Mmus\Slc7a5
1 of 15
No
No
Mmus\Slc7a6
1 of 15
No
No
Mmus\Slc7a7
1 of 15
No
No
Mmus\Slc7a8
1 of 15
No
No
Mmus\Slc7a9
1 of 15
No
No
Mmus\Slc7a10
1 of 15
No
No
Mmus\Slc7a11
1 of 15
No
No
Mmus\Slc7a12
1 of 15
No
No
Mmus\Slc7a13
1 of 15
No
No
Mmus\Slc7a14
1 of 15
No
No
Mmus\Slc7a15
1 of 15
No
No
Mmus\Slc12a8
1 of 15
No
No
Mmus\Slc12a9
1 of 15
No
No
Rattus norvegicus (Norway rat) (25)
Rnor\Slc12a1
12 of 13
Yes
Yes
Rnor\Slc12a2
10 of 13
No
Yes
Rnor\Slc12a3
8 of 13
No
Yes
Rnor\Slc12a4
2 of 13
No
No
Rnor\Slc12a6
2 of 13
No
No
Rnor\Slc12a7
2 of 13
No
No
Rnor\LOC361914
1 of 13
No
No
Rnor\LOC688389
1 of 13
No
No
Rnor\LOC102553442
1 of 13
No
Yes
Rnor\Slc7a1
1 of 13
No
No
Rnor\Slc7a3
1 of 13
No
No
Rnor\Slc7a4
1 of 13
No
No
Rnor\Slc7a5
1 of 13
No
No
Rnor\Slc7a6
1 of 13
No
No
Rnor\Slc7a7
1 of 13
No
No
Rnor\Slc7a8
1 of 13
No
No
Rnor\Slc7a9
1 of 13
No
No
Rnor\Slc7a10
1 of 13
No
No
Rnor\Slc7a11
1 of 13
No
No
Rnor\Slc7a12
1 of 13
No
No
Rnor\Slc7a13
1 of 13
No
No
Rnor\Slc7a14
1 of 13
No
No
Rnor\Slc7a15
1 of 13
No
No
Rnor\Slc12a5
1 of 13
No
No
Rnor\Slc12a8
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (23)
Xtro\slc12a1
7 of 12
Yes
Yes
Xtro\slc12a3
6 of 12
No
Yes
Xtro\slc12a2
3 of 12
No
Yes
Xtro\slc12a3.2
2 of 12
No
Yes
Xtro\slc12a4
2 of 12
No
No
Xtro\slc12a6
2 of 12
No
No
Xtro\LOC100492274
1 of 12
No
No
Xtro\slc7a1
1 of 12
No
No
Xtro\slc7a2.1
1 of 12
No
No
Xtro\slc7a2.2
1 of 12
No
No
Xtro\slc7a3
1 of 12
No
No
Xtro\slc7a4
1 of 12
No
No
Xtro\slc7a5
1 of 12
No
No
Xtro\slc7a6
1 of 12
No
No
Xtro\slc7a7
1 of 12
No
No
Xtro\slc7a8
1 of 12
No
No
Xtro\slc7a9
1 of 12
No
No
Xtro\slc7a10
1 of 12
No
No
Xtro\slc7a11
1 of 12
No
No
Xtro\slc7a13
1 of 12
No
No
Xtro\slc7a14
1 of 12
No
No
Xtro\slc7a15
1 of 12
No
No
Xtro\slc12a9
1 of 12
No
No
Danio rerio (Zebrafish) (34)
Drer\slc12a3
11 of 15
Yes
Yes
Drer\slc12a10.2
9 of 15
No
Yes
Drer\slc12a10.3
8 of 15
No
Yes
Drer\slc12a2
7 of 15
No
Yes
Drer\slc12a10.1
7 of 15
No
Yes
Drer\slc12a1
6 of 15
No
Yes
Drer\LOC100537771
2 of 15
No
Yes
Drer\si:cabz01090193.1
2 of 15
No
No
Drer\si:ch211-59d13.2
2 of 15
No
No
Drer\slc12a4
2 of 15
No
No
Drer\slc12a5b
2 of 15
No
No
Drer\slc12a7a
2 of 15
No
No
Drer\slc12a7b
2 of 15
No
No
Drer\LOC100329477
1 of 15
No
Yes
Drer\si:cabz01102082.1
1 of 15
No
Yes
Drer\si:ch73-352p4.8
1 of 15
No
No
Drer\slc7a1a
1 of 15
No
No
Drer\slc7a3a
1 of 15
No
No
Drer\slc7a3b
1 of 15
No
No
Drer\slc7a4
1 of 15
No
No
Drer\slc7a5
1 of 15
No
No
Drer\slc7a6
1 of 15
No
No
Drer\slc7a7
1 of 15
No
No
Drer\slc7a8a
1 of 15
No
No
Drer\slc7a8b
1 of 15
No
No
Drer\slc7a10a
1 of 15
No
No
Drer\slc7a10b
1 of 15
No
No
Drer\slc7a11
1 of 15
No
No
Drer\slc7a14a
1 of 15
No
No
Drer\slc7a14b
1 of 15
No
No
Drer\slc12a8
1 of 15
No
No
Drer\slc12a9
1 of 15
No
No
Drer\zgc:153039
1 of 15
No
No
Drer\zmp:0000001267
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (20)
Cele\nkcc-1
13 of 15
Yes
Yes
Cele\B0303.11
4 of 15
No
Yes
Cele\aat-1
1 of 15
No
No
Cele\aat-2
1 of 15
No
No
Cele\aat-3
1 of 15
No
No
Cele\aat-4
1 of 15
No
No
Cele\aat-5
1 of 15
No
No
Cele\aat-6
1 of 15
No
No
Cele\aat-7
1 of 15
No
No
Cele\aat-8
1 of 15
No
No
Cele\aat-9
1 of 15
No
No
Cele\B0454.6
1 of 15
No
No
Cele\C50D2.2
1 of 15
No
No
Cele\F10E7.9
1 of 15
No
No
Cele\F23F1.6
1 of 15
No
No
Cele\kcc-1
1 of 15
No
No
Cele\kcc-2
1 of 15
No
No
Cele\kcc-3
1 of 15
No
No
Cele\T04B8.5
1 of 15
No
No
Cele\Y53F4B.12
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (1)
Atha\CCC1
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (25)
Scer\AGP1
1 of 15
Yes
No
Scer\AGP2
1 of 15
Yes
No
Scer\AGP3
1 of 15
Yes
No
Scer\ALP1
1 of 15
Yes
No
Scer\BAP2
1 of 15
Yes
No
Scer\BAP3
1 of 15
Yes
No
Scer\BIO5
1 of 15
Yes
Yes
Scer\CAN1
1 of 15
Yes
No
Scer\DIP5
1 of 15
Yes
No
Scer\GAP1
1 of 15
Yes
No
Scer\GNP1
1 of 15
Yes
No
Scer\HIP1
1 of 15
Yes
No
Scer\HNM1
1 of 15
Yes
Yes
Scer\LYP1
1 of 15
Yes
No
Scer\MMP1
1 of 15
Yes
No
Scer\MUP1
1 of 15
Yes
No
Scer\MUP3
1 of 15
Yes
No
Scer\PUT4
1 of 15
Yes
No
Scer\SAM3
1 of 15
Yes
No
Scer\SSY1
1 of 15
Yes
No
Scer\TAT1
1 of 15
Yes
No
Scer\TAT2
1 of 15
Yes
No
Scer\TPO5
1 of 15
Yes
Yes
Scer\UGA4
1 of 15
Yes
No
Scer\VHC1
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091901JN )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00004121
Drosophila simulans
Dsim\GD12726
Drosophila sechellia
Dsec\GM24663
Drosophila erecta
Dere\GG13837
Drosophila yakuba
Dyak\GE20127
Drosophila ananassae
Dana\GF24985
Drosophila pseudoobscura pseudoobscura
Dpse\GA18131
Drosophila persimilis
Dper\GL24962
Drosophila willistoni
Dwil\GK12754
Drosophila virilis
Dvir\GJ12226
Drosophila mojavensis
Dmoj\GI12335
Drosophila grimshawi
Dgri\GH16072
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500VT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17024898
Glossina morsitans
Tsetse fly
Gmor\GMOY000118
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_14179
Mayetiola destructor
Hessian fly
Mdes\Mdes000307
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL006180
Anopheles darlingi
American malaria mosquito
Adar\ADAC006897
Anopheles gambiae
Malaria mosquito
Agam\30E5.1
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ019752
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01W3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA012368
Bombyx mori
Silkmoth
Bmor\BGIBMGA001473
Bombyx mori
Silkmoth
Bmor\BGIBMGA001472
Danaus plexippus
Monarch butterfly
Dple\DPOGS208044-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS215409-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL010896
Heliconius melpomene
Postman butterfly
Hmel\HMEL017422
Apis florea
Little honeybee
Aflo\4204802836
Apis florea
Little honeybee
Aflo\3962581588
Apis mellifera
Western honey bee
Amel\GB40038
Apis mellifera
Western honey bee
Amel\GB40598
Apis mellifera
Western honey bee
Amel\LOC409715
Apis mellifera
Western honey bee
Amel\LOC552368
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP20032
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP23533
Bombus terrestris
Buff-tailed bumblebee
Bter\15210042
Bombus terrestris
Buff-tailed bumblebee
Bter\15206001
Linepithema humile
Argentine ant
Lhum\LH14510
Linepithema humile
Argentine ant
Lhum\LH21364
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001877
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000721
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000167
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000449
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_11532
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00861
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10956
Tribolium castaneum
Red flour beetle
Tcas\TC030994
Tribolium castaneum
Red flour beetle
Tcas\GLEAN_15462
Pediculus humanus
Human body louse
Phum\PHUM399960
Pediculus humanus
Human body louse
Phum\PHUM399390
Pediculus humanus
Human body louse
Phum\PHUM417670
Rhodnius prolixus
Kissing bug
Rpro\RPRC010778
Rhodnius prolixus
Kissing bug
Rpro\RPRC010561
Rhodnius prolixus
Kissing bug
Rpro\RPRC005132
Rhodnius prolixus
Kissing bug
Rpro\RPRC008828
Cimex lectularius
Bed bug
Clec\CLEC006027
Cimex lectularius
Bed bug
Clec\CLEC011864
Cimex lectularius
Bed bug
Clec\CLEC011102
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI007138
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI069360
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI007154
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001649
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_09032
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_09035
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_09037
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X028Y )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR003421
Strigamia maritima
European centipede
Smar\SMAR000262
Strigamia maritima
European centipede
Smar\SMAR012357
Ixodes scapularis
Black-legged tick
Isca\ISCW001819
Stegodyphus mimosarum
African social velvet spider
Smim\KFM60478.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM59193.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur06g04620
Tetranychus urticae
Two-spotted spider mite
Turt\tetur25g01540
Tetranychus urticae
Two-spotted spider mite
Turt\tetur11g01900
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_203293
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_324603
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_307471
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_324595
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_324617
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_319556
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_252082
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_18728
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_319627
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_324602
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_319643
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04G2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_003287gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Nkcc1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Slc12a2
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000023743
Gallus gallus
Domestic chicken
Ggal\SLC12A2
Gallus gallus
Domestic chicken
Ggal\SLC12A3
Gallus gallus
Domestic chicken
Ggal\SLC12A1
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (15)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
NKCC
6 of 10
CG10413
3 of 10
CG12773
3 of 10
kcc
3 of 10
CG1607
1 of 10
CG5535
1 of 10
CG7255
1 of 10
CG12531
1 of 10
CG13248
1 of 10
gb
1 of 10
JhI-21
1 of 10
mnd
1 of 10
sbm
1 of 10
slif
1 of 10
tadr
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Allele
Disease
Evidence
References
Ncc69GD14863
model of  epilepsy
CEA
(Rusan et al., 2014)
Ncc69JF03097
model of  epilepsy
CEA
(Rusan et al., 2014)
Potential Models Based on Orthology ( 2 )
Modifiers Based on Experimental Evidence ( 2 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
SLC12A2; solute carrier family 12 member 2
15 of 15
600840
     
    SLC12A1; solute carrier family 12 member 1
    13 of 15
    600839
    SLC12A3; solute carrier family 12 member 3
    12 of 15
    600968
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Ncc69 - fray
    enzymatic study, autoradiography, two hybrid
    (Sun et al., 2018, Leiserson et al., 2011)
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Eaat1
    suppressible
    Ncc69
    (Parinejad et al., 2016)
    kcc
    suppressible
    Ncc69
    (Hekmat-Scafe et al., 2010)
    Wnk
    suppressible
    Ncc69
    (Wu et al., 2014)
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map

    3-38

    Cytogenetic map
    69B3-69B3
    Sequence location
    3L:12,368,646..12,391,444 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    69B3-69B3
    Limits computationally determined from genome sequence between P{PZ}l(3)0508805088 and P{PZ}l(3)0692406924
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    3-38

    (FlyBase, 2016)

    3-31.3

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (7)
    Genomic Clones (32)
    cDNA Clones (25)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Sun et al., 2010)
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: Ncc69 CG4357

    (Leiserson, 2005.3.2)
    Source for database merge of

    Source for merge of: CG4357 BEST:CK01027

    (Rana, 2004.7.8)
    Additional comments
    Other Comments

    Identification: as a protein that physically interacts with the N-terminal region of the fray gene product.

    (Leiserson and Keshishian, 2005)

    Identification: cDNA screen for secreted and transmembrane proteins expressed during embryogenesis.

    (Kopczynski et al., 1998)
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 50 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (10)
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (67)