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Gene Dmel\CG10754

General Information
Symbol	Dmel\CG10754	Species	D. melanogaster
Name		Annotation symbol	CG10754
Feature type	protein_coding_gene	FlyBase ID	FBgn0036314
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	69E4-69E4	Sequence location	3L:12,806,128..12,807,119 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG10754 (FBgn0036314). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: zinc ion binding; nucleic acid binding. There is experimental evidence that it is involved in the biological process: mitotic spindle organization; neurogenesis. 3 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: Cactin C-terminal domain; Zinc finger, C2H2-like; Zinc finger, C2H2-type matrin; Zinc finger, U1-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1624603_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG10754. Gene sequence location is 3L:12806128..12807119.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 69E4-69E4   Limits computationally determined from genome sequence between P{PZ}l(3)0692406924 and P{lacW}RpS12s2783
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG10754 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG10754-RA FBtr0075944  NM_140346   992   264
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG10754-PA   FBpp0075676   30.3   264   10.21     NP_648603   AAF49876
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2-type matrin (IPR000690) Zinc finger, U1-type (IPR003604) Zinc finger, C2H2-like (IPR015880) Cactin C-terminal domain (IPR019134)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF49876   AI515570   AI546314   AW943902   AY118970 AAM50830   BG638335   BI579159   BI612705   BI632216   BI638296   BI639518   BX039355   BX041519   BX042970   BX045721   BX045860   BX049815   BX052279   BX052478   BX053118   BX064752   BX068895   CO300286   CO343880   EC229257   EC246033   EC256235   FE863468   FE863742   FE863795   GH744617   GH746944   GH764500   GH768484   GH779210   GH779377
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VU15
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
catalytic step 2 spliceosome inferred from direct assay (Herold et al., 2009) precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    348.575 Larval Midgut    85.3 Larval Hindgut    157.2 Larval Malpighian Tubules    141.5 Larval Fat Body    131.3 Larval Salivary Gland    205.3 Larval Trachea    164.325 Larval Carcass    158.3 Adult Head    93.7 Adult Eye    103.85 Adult Brain    122.3 Adult Thoracic-Abdominal Ganglion    116.3 Adult Crop    140.7 Adult Midgut    112 Adult Hindgut    140 Adult Malpighian Tubules    102.2 Adult Fat Body    95 Adult Salivary Gland    162.7 Adult Heart    83 Adult VirginFemale Spermatheca    74.3 Adult InseminatedFemale Spermatheca    87.3 Adult Ovary    202.9 Adult Testis    84.2 Adult Male Accessory Gland    215.5 Adult Carcass    88.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    348.575 Larval Midgut    85.3 Larval Hindgut    157.2 Larval Malpighian Tubules    141.5 Larval Fat Body    131.3 Larval Salivary Gland    205.3 Larval Trachea    164.325 Larval Carcass    158.3 Adult Head    93.7 Adult Eye    103.85 Adult Brain    122.3 Adult Thoracic-Abdominal Ganglion    116.3 Adult Crop    140.7 Adult Midgut    112 Adult Hindgut    140 Adult Malpighian Tubules    102.2 Adult Fat Body    95 Adult Salivary Gland    162.7 Adult Heart    83 Adult VirginFemale Spermatheca    74.3 Adult InseminatedFemale Spermatheca    87.3 Adult Ovary    202.9 Adult Testis    84.2 Adult Male Accessory Gland    215.5 Adult Carcass    88.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    348.575 Larval Midgut    85.3 Larval Hindgut    157.2 Larval Malpighian Tubules    141.5 Larval Fat Body    131.3 Larval Salivary Gland    205.3 Larval Trachea    164.325 Larval Carcass    158.3 Adult Head    93.7 Adult Eye    103.85 Adult Brain    122.3 Adult Thoracic-Abdominal Ganglion    116.3 Adult Crop    140.7 Adult Midgut    112 Adult Hindgut    140 Adult Malpighian Tubules    102.2 Adult Fat Body    95 Adult Salivary Gland    162.7 Adult Heart    83 Adult VirginFemale Spermatheca    74.3 Adult InseminatedFemale Spermatheca    87.3 Adult Ovary    202.9 Adult Testis    84.2 Adult Male Accessory Gland    215.5 Adult Carcass    88.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    348.575 Larval Midgut    85.3 Larval Hindgut    157.2 Larval Malpighian Tubules    141.5 Larval Fat Body    131.3 Larval Salivary Gland    205.3 Larval Trachea    164.325 Larval Carcass    158.3 Adult Head    93.7 Adult Eye    103.85 Adult Brain    122.3 Adult Thoracic-Abdominal Ganglion    116.3 Adult Crop    140.7 Adult Midgut    112 Adult Hindgut    140 Adult Malpighian Tubules    102.2 Adult Fat Body    95 Adult Salivary Gland    162.7 Adult Heart    83 Adult VirginFemale Spermatheca    74.3 Adult InseminatedFemale Spermatheca    87.3 Adult Ovary    202.9 Adult Testis    84.2 Adult Male Accessory Gland    215.5 Adult Carcass    88.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    348.575 Larval Midgut    85.3 Larval Hindgut    157.2 Larval Malpighian Tubules    141.5 Larval Fat Body    131.3 Larval Salivary Gland    205.3 Larval Trachea    164.325 Larval Carcass    158.3 Adult Head    93.7 Adult Eye    103.85 Adult Brain    122.3 Adult Thoracic-Abdominal Ganglion    116.3 Adult Crop    140.7 Adult Midgut    112 Adult Hindgut    140 Adult Malpighian Tubules    102.2 Adult Fat Body    95 Adult Salivary Gland    162.7 Adult Heart    83 Adult VirginFemale Spermatheca    74.3 Adult InseminatedFemale Spermatheca    87.3 Adult Ovary    202.9 Adult Testis    84.2 Adult Male Accessory Gland    215.5 Adult Carcass    88.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    348.575 Larval Midgut    85.3 Larval Hindgut    157.2 Larval Malpighian Tubules    141.5 Larval Fat Body    131.3 Larval Salivary Gland    205.3 Larval Trachea    164.325 Larval Carcass    158.3 Adult Head    93.7 Adult Eye    103.85 Adult Brain    122.3 Adult Thoracic-Abdominal Ganglion    116.3 Adult Crop    140.7 Adult Midgut    112 Adult Hindgut    140 Adult Malpighian Tubules    102.2 Adult Fat Body    95 Adult Salivary Gland    162.7 Adult Heart    83 Adult VirginFemale Spermatheca    74.3 Adult InseminatedFemale Spermatheca    87.3 Adult Ovary    202.9 Adult Testis    84.2 Adult Male Accessory Gland    215.5 Adult Carcass    88.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    348.575 Larval Midgut    85.3 Larval Hindgut    157.2 Larval Malpighian Tubules    141.5 Larval Fat Body    131.3 Larval Salivary Gland    205.3 Larval Trachea    164.325 Larval Carcass    158.3 Adult Head    93.7 Adult Eye    103.85 Adult Brain    122.3 Adult Thoracic-Abdominal Ganglion    116.3 Adult Crop    140.7 Adult Midgut    112 Adult Hindgut    140 Adult Malpighian Tubules    102.2 Adult Fat Body    95 Adult Salivary Gland    162.7 Adult Heart    83 Adult VirginFemale Spermatheca    74.3 Adult InseminatedFemale Spermatheca    87.3 Adult Ovary    202.9 Adult Testis    84.2 Adult Male Accessory Gland    215.5 Adult Carcass    88.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    348.575 Larval Midgut    85.3 Larval Hindgut    157.2 Larval Malpighian Tubules    141.5 Larval Fat Body    131.3 Larval Salivary Gland    205.3 Larval Trachea    164.325 Larval Carcass    158.3 Adult Head    93.7 Adult Eye    103.85 Adult Brain    122.3 Adult Thoracic-Abdominal Ganglion    116.3 Adult Crop    140.7 Adult Midgut    112 Adult Hindgut    140 Adult Malpighian Tubules    102.2 Adult Fat Body    95 Adult Salivary Gland    162.7 Adult Heart    83 Adult VirginFemale Spermatheca    74.3 Adult InseminatedFemale Spermatheca    87.3 Adult Ovary    202.9 Adult Testis    84.2 Adult Male Accessory Gland    215.5 Adult Carcass    88.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    93.7   NA  Eye    103.85   NA  Brain    122.3   348.575  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    116.3   NA  Crop    140.7   85.3  Midgut    112   157.2  Hindgut    140   141.5  Malpighian Tubules    102.2   131.3  Fat Body    95   205.3  Salivary Gland    162.7   NA  Heart    83   164.325  Trachea    NA   NA  VirginFemale Spermatheca    74.3   NA  InseminatedFemale Spermatheca    87.3   NA  Ovary    202.9   NA  Testis    84.2   NA  Male Accessory Gland    215.5   158.3  Carcass    88.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    39 central nervous system, pupae P8    15 head, virgin 1-day female    7 head, virgin 4-day female    9 head, virgin 20-day female    10 head, mated 1-day female    8 head, mated 4-day female    6 head, mated 20-day female    11 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    11 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    9 digestive system, 20-day adult    11 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    19 carcass, larvae L3 wandering    28 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    11 ovary, virgin 4-day female    31 ovary, mated 4-day female    22 testis, mated 4-day male    26 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (51) central nervous system, larvae L3  (39) central nervous system, pupae P8    15 head, virgin 1-day female    7 head, virgin 4-day female    9 head, virgin 20-day female    10 head, mated 1-day female    8 head, mated 4-day female    6 head, mated 20-day female    11 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    11 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    9 digestive system, 20-day adult    11 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    19 carcass, larvae L3 wandering  (28) carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    11 ovary, virgin 4-day female  (31) ovary, mated 4-day female    22 testis, mated 4-day male  26 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    39 central nervous system, pupae P8    15 head, virgin 1-day female    7 head, virgin 4-day female    9 head, virgin 20-day female    10 head, mated 1-day female    8 head, mated 4-day female    6 head, mated 20-day female    11 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    11 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    9 digestive system, 20-day adult    11 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    19 carcass, larvae L3 wandering    28 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    11 ovary, virgin 4-day female    31 ovary, mated 4-day female    22 testis, mated 4-day male    26 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    39 central nervous system, pupae P8    15 head, virgin 1-day female    7 head, virgin 4-day female    9 head, virgin 20-day female    10 head, mated 1-day female    8 head, mated 4-day female    6 head, mated 20-day female    11 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    11 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    9 digestive system, 20-day adult    11 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    19 carcass, larvae L3 wandering    28 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    11 ovary, virgin 4-day female    31 ovary, mated 4-day female    22 testis, mated 4-day male    26 accessory gland, mated 4-day male    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    39 central nervous system, pupae P8    15 head, virgin 1-day female    7 head, virgin 4-day female    9 head, virgin 20-day female    10 head, mated 1-day female    8 head, mated 4-day female    6 head, mated 20-day female    11 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    11 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    9 digestive system, 20-day adult    11 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    19 carcass, larvae L3 wandering    28 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    11 ovary, virgin 4-day female    31 ovary, mated 4-day female    22 testis, mated 4-day male    26 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (51) central nervous system, larvae L3  (39) central nervous system, pupae P8    15 head, virgin 1-day female    7 head, virgin 4-day female    9 head, virgin 20-day female    10 head, mated 1-day female    8 head, mated 4-day female    6 head, mated 20-day female    11 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    11 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    9 digestive system, 20-day adult    11 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    19 carcass, larvae L3 wandering  28 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    11 ovary, virgin 4-day female  31 ovary, mated 4-day female    22 testis, mated 4-day male  26 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    39 central nervous system, pupae P8    15 head, virgin 1-day female    7 head, virgin 4-day female    9 head, virgin 20-day female    10 head, mated 1-day female    8 head, mated 4-day female    6 head, mated 20-day female    11 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    11 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    9 digestive system, 20-day adult    11 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    19 carcass, larvae L3 wandering    28 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    11 ovary, virgin 4-day female    31 ovary, mated 4-day female    22 testis, mated 4-day male    26 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    39 central nervous system, pupae P8    15 head, virgin 1-day female    7 head, virgin 4-day female    9 head, virgin 20-day female    10 head, mated 1-day female    8 head, mated 4-day female    6 head, mated 20-day female    11 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    11 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    9 digestive system, 20-day adult    11 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    19 carcass, larvae L3 wandering    28 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    11 ovary, virgin 4-day female    31 ovary, mated 4-day female    22 testis, mated 4-day male    26 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    55 embryo 02-04hr    97 embryo 04-06hr    109 embryo 06-08hr    95 embryo 08-10hr    73 embryo 10-12hr    61 embryo 12-14hr    45 embryo 14-16hr    42 embryo 16-18hr    32 embryo 18-20hr    28 embryo 20-22hr    28 embryo 22-24hr    26 larva L1    27 larva L2    29 larva L3 12hr old    20 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    31 white prepupae 12hr    29 white prepupae 24hr    38 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    12 adult male 01day    13 adult male 05day    16 adult male 30day    14 adult female 01day    24 adult female 05day    35 adult female 30day    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (55) embryo 02-04hr  (97) embryo 04-06hr  (109) embryo 06-08hr  (95) embryo 08-10hr  (73) embryo 10-12hr  (61) embryo 12-14hr  (45) embryo 14-16hr  (42) embryo 16-18hr  (32) embryo 18-20hr  (28) embryo 20-22hr  (28) embryo 22-24hr  26 larva L1  (27) larva L2  (29) larva L3 12hr old    20 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9  (28) white prepupae new  (31) white prepupae 12hr  (29) white prepupae 24hr  (38) pupae 2d postWPP  26 pupae 3d postWPP    16 pupae 4d postWPP    12 adult male 01day    13 adult male 05day    16 adult male 30day    14 adult female 01day    24 adult female 05day  (35) adult female 30day  (35) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    55 embryo 02-04hr    97 embryo 04-06hr  (109) embryo 06-08hr    95 embryo 08-10hr    73 embryo 10-12hr    61 embryo 12-14hr    45 embryo 14-16hr    42 embryo 16-18hr    32 embryo 18-20hr    28 embryo 20-22hr    28 embryo 22-24hr    26 larva L1    27 larva L2    29 larva L3 12hr old    20 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    31 white prepupae 12hr    29 white prepupae 24hr    38 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    12 adult male 01day    13 adult male 05day    16 adult male 30day    14 adult female 01day    24 adult female 05day    35 adult female 30day    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    55 embryo 02-04hr    97 embryo 04-06hr    109 embryo 06-08hr    95 embryo 08-10hr    73 embryo 10-12hr    61 embryo 12-14hr    45 embryo 14-16hr    42 embryo 16-18hr    32 embryo 18-20hr    28 embryo 20-22hr    28 embryo 22-24hr    26 larva L1    27 larva L2    29 larva L3 12hr old    20 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    31 white prepupae 12hr    29 white prepupae 24hr    38 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    12 adult male 01day    13 adult male 05day    16 adult male 30day    14 adult female 01day    24 adult female 05day    35 adult female 30day    35 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    55 embryo 02-04hr    97 embryo 04-06hr    109 embryo 06-08hr    95 embryo 08-10hr    73 embryo 10-12hr    61 embryo 12-14hr    45 embryo 14-16hr    42 embryo 16-18hr    32 embryo 18-20hr    28 embryo 20-22hr    28 embryo 22-24hr    26 larva L1    27 larva L2    29 larva L3 12hr old    20 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    31 white prepupae 12hr    29 white prepupae 24hr    38 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    12 adult male 01day    13 adult male 05day    16 adult male 30day    14 adult female 01day    24 adult female 05day    35 adult female 30day    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (55) embryo 02-04hr  (97) embryo 04-06hr  (109) embryo 06-08hr  (95) embryo 08-10hr  (73) embryo 10-12hr  (61) embryo 12-14hr  (45) embryo 14-16hr  (42) embryo 16-18hr  32 embryo 18-20hr  28 embryo 20-22hr  28 embryo 22-24hr  26 larva L1  27 larva L2  29 larva L3 12hr old    20 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9  28 white prepupae new  31 white prepupae 12hr  29 white prepupae 24hr  (38) pupae 2d postWPP  26 pupae 3d postWPP    16 pupae 4d postWPP    12 adult male 01day    13 adult male 05day    16 adult male 30day    14 adult female 01day    24 adult female 05day  (35) adult female 30day  (35) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    55 embryo 02-04hr    97 embryo 04-06hr  109 embryo 06-08hr    95 embryo 08-10hr    73 embryo 10-12hr    61 embryo 12-14hr    45 embryo 14-16hr    42 embryo 16-18hr    32 embryo 18-20hr    28 embryo 20-22hr    28 embryo 22-24hr    26 larva L1    27 larva L2    29 larva L3 12hr old    20 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    31 white prepupae 12hr    29 white prepupae 24hr    38 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    12 adult male 01day    13 adult male 05day    16 adult male 30day    14 adult female 01day    24 adult female 05day    35 adult female 30day    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    55 embryo 02-04hr    97 embryo 04-06hr    109 embryo 06-08hr    95 embryo 08-10hr    73 embryo 10-12hr    61 embryo 12-14hr    45 embryo 14-16hr    42 embryo 16-18hr    32 embryo 18-20hr    28 embryo 20-22hr    28 embryo 22-24hr    26 larva L1    27 larva L2    29 larva L3 12hr old    20 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    31 white prepupae 12hr    29 white prepupae 24hr    38 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    12 adult male 01day    13 adult male 05day    16 adult male 30day    14 adult female 01day    24 adult female 05day    35 adult female 30day    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    37 embryonic 1182-4H    27 embryonic GM2    18 embryonic Kc167    18 embryonic S1    58 embryonic S3    33 leg disc CME L1    61 wing disc CME-W2    29 wing disc ML-DmD8    32 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    38 wing disc ML-DmD21    60 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    83 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    45 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    25 ovary fGS/OSS    50 ovary OSC    36 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (33) Schneider line 2 Sg4  (37) embryonic 1182-4H  (27) embryonic GM2    18 embryonic Kc167    18 embryonic S1  (58) embryonic S3  (33) leg disc CME L1  (61) wing disc CME-W2  (29) wing disc ML-DmD8  (32) wing disc ML-DmD9  (65) wing disc ML-DmD16-c3  (38) wing disc ML-DmD21  (60) wing disc ML-DmD32  (28) haltere disc ML-DmD17-c3  (83) eye-antennal disc ML-DmD11  (34) antennal disc ML-DmD20-c5  (45) mixed discs ML-DmD4-c1  (34) CNS ML-DmBG1-c1  (45) CNS ML-DmBG2-c2  (31) tumorous blood cells mbn2    25 ovary fGS/OSS  (50) ovary OSC  (36) ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    37 embryonic 1182-4H    27 embryonic GM2    18 embryonic Kc167    18 embryonic S1    58 embryonic S3    33 leg disc CME L1    61 wing disc CME-W2    29 wing disc ML-DmD8    32 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    38 wing disc ML-DmD21    60 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    83 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    45 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    25 ovary fGS/OSS    50 ovary OSC    36 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    37 embryonic 1182-4H    27 embryonic GM2    18 embryonic Kc167    18 embryonic S1    58 embryonic S3    33 leg disc CME L1    61 wing disc CME-W2    29 wing disc ML-DmD8    32 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    38 wing disc ML-DmD21    60 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    83 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    45 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    25 ovary fGS/OSS    50 ovary OSC    36 ovary OSS    18 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    37 embryonic 1182-4H    27 embryonic GM2    18 embryonic Kc167    18 embryonic S1    58 embryonic S3    33 leg disc CME L1    61 wing disc CME-W2    29 wing disc ML-DmD8    32 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    38 wing disc ML-DmD21    60 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    83 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    45 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    25 ovary fGS/OSS    50 ovary OSC    36 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (33) Schneider line 2 Sg4  (37) embryonic 1182-4H  27 embryonic GM2    18 embryonic Kc167    18 embryonic S1  (58) embryonic S3  (33) leg disc CME L1  (61) wing disc CME-W2  29 wing disc ML-DmD8  32 wing disc ML-DmD9  (65) wing disc ML-DmD16-c3  (38) wing disc ML-DmD21  (60) wing disc ML-DmD32  28 haltere disc ML-DmD17-c3  (83) eye-antennal disc ML-DmD11  (34) antennal disc ML-DmD20-c5  (45) mixed discs ML-DmD4-c1  (34) CNS ML-DmBG1-c1  (45) CNS ML-DmBG2-c2  31 tumorous blood cells mbn2    25 ovary fGS/OSS  (50) ovary OSC  (36) ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    37 embryonic 1182-4H    27 embryonic GM2    18 embryonic Kc167    18 embryonic S1    58 embryonic S3    33 leg disc CME L1    61 wing disc CME-W2    29 wing disc ML-DmD8    32 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    38 wing disc ML-DmD21    60 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    83 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    45 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    25 ovary fGS/OSS    50 ovary OSC    36 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    37 embryonic 1182-4H    27 embryonic GM2    18 embryonic Kc167    18 embryonic S1    58 embryonic S3    33 leg disc CME L1    61 wing disc CME-W2    29 wing disc ML-DmD8    32 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    38 wing disc ML-DmD21    60 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    83 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    45 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    45 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    25 ovary fGS/OSS    50 ovary OSC    36 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    11 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    11 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3  (27) Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    11 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    11 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    11 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    11 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3  27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    11 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    11 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD47455 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0036314 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10754-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for CG10754 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal CG10754GD7087 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 CG10754GD7087 Other Phenotypes Allele neuroanatomy defective | larval stage CG10754GD7087 visible, with Scer\GAL4pnr-MD237 CG10754GD7087 Phenotype manifest in Allele embryonic/larval neuroblast | larval stage CG10754GD7087 ganglion mother cell | larval stage CG10754GD7087 mesothoracic tergum, with Scer\GAL4pnr-MD237 CG10754GD7087
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG10754 Class Mutagen Stocks Known lesion CG10754f03161 piggyBac transposase 1 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG10754 Class Mutagen Stocks Known lesion CG10754GD7087 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CG10754NIG.10754R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7087} NA P{NIG.10754R} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}CG10754f03161 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
catalytic step 2 spliceosome inferred from direct assay (Herold et al., 2009) precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
nucleic acid binding inferred from electronic annotation with InterPro:IPR000690, InterPro:IPR003604 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR000690, InterPro:IPR003604, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
mRNA splicing, via spliceosome inferred by curator from GO:0071011|GO:0071013 (Herold et al., 2009) inferred from sequence or structural similarity with human SF3A2 (FlyBase, 1992-) inferred from sequence or structural similarity with NCBI:NP_038679 AND inferred from sequence or structural similarity with Saccharomyces PRP11 (Mount and Salz, 2000)
Cellular Component
CV term Evidence References
spliceosomal complex inferred from sequence or structural similarity with human SF3A2 (FlyBase, 1992-) U2 snRNP inferred from sequence or structural similarity with NCBI:NP_038679 AND inferred from sequence or structural similarity with Saccharomyces PRP11 (Mount and Salz, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
CG10754 - CG16941 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) CG10754 - Df31 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG10754 - PH4αEFB anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG10754 - noi anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG10754 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64803 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0036314 DroID - A comprehensive database of gene and protein interactions. • FBgn0036314 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39456
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6V17M4 Dipteran inclusive ortholog search EOG6TB4MC Insect inclusive ortholog search EOG6GHXK8 Arthropod inclusive ortholog search EOG66WWRJ Metazoa inclusive ortholog search EOG62VN9P
Orthologs in Drosophila Species (EOG6V17M4)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG10754     Drosophila simulans   Dsim\GD12694 Y   Drosophila sechellia   Dsec\GM24628 Y   Drosophila erecta   Dere\GG13802 Y   Drosophila yakuba   Dyak\GE20095 Y   Drosophila ananassae   Dana\GF10865 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10545 Y   Drosophila persimilis   Dper\GL22896 Y   Drosophila willistoni   Dwil\GK16828 Y   Drosophila virilis   Dvir\GJ13983 Y   Drosophila mojavensis   Dmoj\GI13650 Y   Drosophila grimshawi   Dgri\GH14494 Y
Orthologs in non-Drosophila Dipterans (EOG6TB4MC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL000394   Anopheles gambiae Malaria mosquito  Agam\AGAP011035   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003364
Orthologs in non-Dipteran Insects (EOG6GHXK8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC552334   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012248   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10211   Atta cephalotes Leafcutter ant  Acep\ACEP20778   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22473   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23263   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23040   Linepithema humile Argentine ant  Lhum\LH23425   Pogonomyrmex barbatus Red harvester ant  Pbar\PB25729   Solenopsis invicta Red fire ant  Sinv\SINV12486   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000572   Bombyx mori Silkmoth  Bmor\BGIBMGA000898   Pediculus humanus Human body louse  Phum\PHUM544530   Tribolium castaneum Red flour beetle  Tcas\GLEAN_07585
Orthologs in non-Insect Arthropods (EOG66WWRJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\rbcL   Ixodes scapularis Deer tick  Isca\ISCW017352
Orthologs in non-Arthropod Metazoa (EOG62VN9P)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\repo-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011993gn   Danio rerio Zebrafish  Drer\sf3a2   Xenopus tropicalis Western clawed frog  Xtro\sf3a2   Mus musculus House mouse  Mmus\Sf3a2   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000021639   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000038467   Rattus norvegicus Norway rat  Rnor\Sf3a2   Homo sapiens Human  Hsap\SF3A2
Human Orthologs (1)
Gene OMIM HGNC Hsap\SF3A2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD12694 Drosophila sechellia Dsec\GM24628 Drosophila erecta Dere\GG13802 Drosophila yakuba Dyak\GE20095 Drosophila ananassae Dana\GF10865 Drosophila pseudoobscura pseudoobscura Dpse\GA10545 Drosophila persimilis Dper\GL22896 Drosophila willistoni Dwil\GK16828 Drosophila virilis Dvir\GJ13983 Drosophila mojavensis Dmoj\GI13650 Drosophila grimshawi Dgri\GH14494
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Harvard	- PBac{WH}CG10754f03161
VDRC	v31346 w1118; P{GD7087}v31346 v31347 w1118; P{GD7087}v31347/CyO
Genomic Clones ( 2 )
	BACR20B01 BACR20I01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 23 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD47455
Other clones	DPiM_LD47455
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD47860 RE73145 RH41649 SD20549 SD22113 SD25882
Other clones	57207 214531.33 CK01.23A.B8 EK177835 EP18262 LD22758 RM07673 RM07873 RM08173 RP001020425 RP001033464 RP001035370 RP001036755 RP001047342 RP001051705
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0036314 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39456
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in a long metaphase spindle with misaligned chromosomes when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140346 RefSeq (Proteins) • NP_648603 DDBJ / EMBL / GenBank • AAF49876 AAM50830 AI515570 AW943902 AY118970 Entrez Gene - A searchable database of RefSeq genes. • 39456 UniProtKB/TrEMBL • Q9VU15
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2-type matrin (IPR000690) Zinc finger, U1-type (IPR003604) Zinc finger, C2H2-like (IPR015880) Cactin C-terminal domain (IPR019134) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD47455
Linkouts
	BioGRID - A database of protein and genetic interactions • 64803 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0036314 DroID - A comprehensive database of gene and protein interactions. • FBgn0036314 DRSC - Results from RNAi screens. • FBgn0036314 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0036314 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10754-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0036314 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39456 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39456 modMine - Data generated by the modENCODE project. • FBgn0036314
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	CG10754 (Goshima et al., 2007, Somma et al., 2008, Edwards and Mackay, 2009, Herold et al., 2009, Neumüller et al., 2011, Japanese National Institute of Genetics, 2012.5.21)
Name Synonym
Secondary FlyBase IDs
References ( 24 )
Generate a list of	All references relating to this gene ( 24 )
List References by type	Research paper  ( 6 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 5 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 1 )
	Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...