Gene Dmel\CG10516

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General Information

Symbol

Dmel\CG10516

Species

D. melanogaster

Name

Annotation symbol

CG10516

Feature type

protein_coding_gene

FlyBase ID

FBgn0036549

Gene Model Status

Current

Stock availability

1 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

72C1-72C1

Sequence location

3L:15,961,304..15,963,002 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG10516 (FBgn0036549). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. The biological processes in which it is involved are not known. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: B30.2/SPRY domain; Concanavalin A-like lectin/glucanases superfamily; SPla/RYanodine receptor SPRY. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult Malpighian tubules, adult fat body, virgin spermatheca. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval Malpighian tubules, adult heart, inseminated spermatheca, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1623666_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG10516. Gene sequence location is 3L:15961304..15963002.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

72C1-72C1

Limits computationally determined from genome sequence between P{EP}Tfb2^EP572 and P{lacW}th^j5C8&P{PZ}Mbs⁰³⁸⁰²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG10516 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG10516-RA

FBtr0075512

NM_140562

1329

342

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG10516-PA

FBpp0075267

38.6

342

6.37

NP_648819

AAF49563

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

B30.2/SPRY domain (IPR001870)

SPla/RYanodine receptor SPRY (IPR003877)

Concanavalin A-like lectin/glucanases superfamily (IPR008985)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VUW0

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult Malpighian tubules, adult fat body, virgin spermatheca. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval Malpighian tubules, adult heart, inseminated spermatheca, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		65.4
Larval Midgut		34.8
Larval Hindgut		34
Larval Malpighian Tubules		321.8
Larval Fat Body		56.2
Larval Salivary Gland		57.5
Larval Trachea		63.8
Larval Carcass		179.925
Adult Head		116.7
Adult Eye		74.875
Adult Brain		49.6
Adult Thoracic-Abdominal Ganglion		39
Adult Crop		48.2
Adult Midgut		41.9
Adult Hindgut		56.1
Adult Malpighian Tubules		2069
Adult Fat Body		508.9
Adult Salivary Gland		56.3
Adult Heart		271.1
Adult VirginFemale Spermatheca		670.2
Adult InseminatedFemale Spermatheca		475.2
Adult Ovary		57.6
Adult Testis		83.1
Adult Male Accessory Gland		55.5
Adult Carcass		267.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		65.4
Larval Midgut		34.8
Larval Hindgut		34
Larval Malpighian Tubules		321.8
Larval Fat Body		56.2
Larval Salivary Gland		57.5
Larval Trachea		63.8
Larval Carcass		179.925
Adult Head		116.7
Adult Eye		74.875
Adult Brain		49.6
Adult Thoracic-Abdominal Ganglion		39
Adult Crop		48.2
Adult Midgut		41.9
Adult Hindgut		56.1
Adult Malpighian Tubules		(2069)
Adult Fat Body		508.9
Adult Salivary Gland		56.3
Adult Heart		271.1
Adult VirginFemale Spermatheca		(670.2)
Adult InseminatedFemale Spermatheca		475.2
Adult Ovary		57.6
Adult Testis		83.1
Adult Male Accessory Gland		55.5
Adult Carcass		267.8
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		65.4
Larval Midgut		34.8
Larval Hindgut		34
Larval Malpighian Tubules		321.8
Larval Fat Body		56.2
Larval Salivary Gland		57.5
Larval Trachea		63.8
Larval Carcass		179.925
Adult Head		116.7
Adult Eye		74.875
Adult Brain		49.6
Adult Thoracic-Abdominal Ganglion		39
Adult Crop		48.2
Adult Midgut		41.9
Adult Hindgut		56.1
Adult Malpighian Tubules		(2069)
Adult Fat Body		508.9
Adult Salivary Gland		56.3
Adult Heart		271.1
Adult VirginFemale Spermatheca		670.2
Adult InseminatedFemale Spermatheca		475.2
Adult Ovary		57.6
Adult Testis		83.1
Adult Male Accessory Gland		55.5
Adult Carcass		267.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		65.4
Larval Midgut		34.8
Larval Hindgut		34
Larval Malpighian Tubules		321.8
Larval Fat Body		56.2
Larval Salivary Gland		57.5
Larval Trachea		63.8
Larval Carcass		179.925
Adult Head		116.7
Adult Eye		74.875
Adult Brain		49.6
Adult Thoracic-Abdominal Ganglion		39
Adult Crop		48.2
Adult Midgut		41.9
Adult Hindgut		56.1
Adult Malpighian Tubules		2069
Adult Fat Body		508.9
Adult Salivary Gland		56.3
Adult Heart		271.1
Adult VirginFemale Spermatheca		670.2
Adult InseminatedFemale Spermatheca		475.2
Adult Ovary		57.6
Adult Testis		83.1
Adult Male Accessory Gland		55.5
Adult Carcass		267.8
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		65.4
Larval Midgut		34.8
Larval Hindgut		34
Larval Malpighian Tubules		321.8
Larval Fat Body		56.2
Larval Salivary Gland		57.5
Larval Trachea		63.8
Larval Carcass		179.925
Adult Head		116.7
Adult Eye		74.875
Adult Brain		49.6
Adult Thoracic-Abdominal Ganglion		39
Adult Crop		48.2
Adult Midgut		41.9
Adult Hindgut		56.1
Adult Malpighian Tubules		2069
Adult Fat Body		508.9
Adult Salivary Gland		56.3
Adult Heart		271.1
Adult VirginFemale Spermatheca		670.2
Adult InseminatedFemale Spermatheca		475.2
Adult Ovary		57.6
Adult Testis		83.1
Adult Male Accessory Gland		55.5
Adult Carcass		267.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		65.4
Larval Midgut		34.8
Larval Hindgut		34
Larval Malpighian Tubules		321.8
Larval Fat Body		56.2
Larval Salivary Gland		57.5
Larval Trachea		63.8
Larval Carcass		179.925
Adult Head		116.7
Adult Eye		74.875
Adult Brain		49.6
Adult Thoracic-Abdominal Ganglion		39
Adult Crop		48.2
Adult Midgut		41.9
Adult Hindgut		56.1
Adult Malpighian Tubules		(2069)
Adult Fat Body		508.9
Adult Salivary Gland		56.3
Adult Heart		271.1
Adult VirginFemale Spermatheca		670.2
Adult InseminatedFemale Spermatheca		475.2
Adult Ovary		57.6
Adult Testis		83.1
Adult Male Accessory Gland		55.5
Adult Carcass		267.8
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		65.4
Larval Midgut		34.8
Larval Hindgut		34
Larval Malpighian Tubules		321.8
Larval Fat Body		56.2
Larval Salivary Gland		57.5
Larval Trachea		63.8
Larval Carcass		179.925
Adult Head		116.7
Adult Eye		74.875
Adult Brain		49.6
Adult Thoracic-Abdominal Ganglion		39
Adult Crop		48.2
Adult Midgut		41.9
Adult Hindgut		56.1
Adult Malpighian Tubules		(2069)
Adult Fat Body		508.9
Adult Salivary Gland		56.3
Adult Heart		271.1
Adult VirginFemale Spermatheca		670.2
Adult InseminatedFemale Spermatheca		475.2
Adult Ovary		57.6
Adult Testis		83.1
Adult Male Accessory Gland		55.5
Adult Carcass		267.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		65.4
Larval Midgut		34.8
Larval Hindgut		34
Larval Malpighian Tubules		321.8
Larval Fat Body		56.2
Larval Salivary Gland		57.5
Larval Trachea		63.8
Larval Carcass		179.925
Adult Head		116.7
Adult Eye		74.875
Adult Brain		49.6
Adult Thoracic-Abdominal Ganglion		39
Adult Crop		48.2
Adult Midgut		41.9
Adult Hindgut		56.1
Adult Malpighian Tubules		2069
Adult Fat Body		508.9
Adult Salivary Gland		56.3
Adult Heart		271.1
Adult VirginFemale Spermatheca		670.2
Adult InseminatedFemale Spermatheca		475.2
Adult Ovary		57.6
Adult Testis		83.1
Adult Male Accessory Gland		55.5
Adult Carcass		267.8
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	116.7
NA	Eye	74.875
NA	Brain	49.6
65.4	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	39
NA	Crop	48.2
34.8	Midgut	41.9
34	Hindgut	56.1
321.8	Malpighian Tubules	2069
56.2	Fat Body	508.9
57.5	Salivary Gland	56.3
NA	Heart	271.1
63.8	Trachea	NA
NA	VirginFemale Spermatheca	670.2
NA	InseminatedFemale Spermatheca	475.2
NA	Ovary	57.6
NA	Testis	83.1
NA	Male Accessory Gland	55.5
179.925	Carcass	267.8

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		4
central nervous system, pupae P8		5
head, virgin 1-day female		22
head, virgin 4-day female		6
head, virgin 20-day female		10
head, mated 1-day female		9
head, mated 4-day female		22
head, mated 20-day female		7
head, mated 1-day male		9
head, mated 4-day male		23
head, mated 20-day male		12
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		18
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		19
digestive system, 4-day adult		14
digestive system, 20-day adult		13
fat body, larvae L3 wandering		1
fat body, white prepupae		14
fat body, pupae P8		21
carcass, larvae L3 wandering		36
carcass, 1-day adult		22
carcass, 4-day adult		18
carcass, 20-day adult		15
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		17
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		4
central nervous system, pupae P8		5
head, virgin 1-day female		22
head, virgin 4-day female		6
head, virgin 20-day female		10
head, mated 1-day female		9
head, mated 4-day female		22
head, mated 20-day female		7
head, mated 1-day male		9
head, mated 4-day male		23
head, mated 20-day male		12
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		18
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		19
digestive system, 4-day adult		14
digestive system, 20-day adult		13
fat body, larvae L3 wandering		1
fat body, white prepupae		14
fat body, pupae P8		21
carcass, larvae L3 wandering		(36)
carcass, 1-day adult		22
carcass, 4-day adult		18
carcass, 20-day adult		15
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		17
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		4
central nervous system, pupae P8		5
head, virgin 1-day female		22
head, virgin 4-day female		6
head, virgin 20-day female		10
head, mated 1-day female		9
head, mated 4-day female		22
head, mated 20-day female		7
head, mated 1-day male		9
head, mated 4-day male		23
head, mated 20-day male		12
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		18
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		19
digestive system, 4-day adult		14
digestive system, 20-day adult		13
fat body, larvae L3 wandering		1
fat body, white prepupae		14
fat body, pupae P8		21
carcass, larvae L3 wandering		36
carcass, 1-day adult		22
carcass, 4-day adult		18
carcass, 20-day adult		15
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		17
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		4
central nervous system, pupae P8		5
head, virgin 1-day female		22
head, virgin 4-day female		6
head, virgin 20-day female		10
head, mated 1-day female		9
head, mated 4-day female		22
head, mated 20-day female		7
head, mated 1-day male		9
head, mated 4-day male		23
head, mated 20-day male		12
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		18
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		19
digestive system, 4-day adult		14
digestive system, 20-day adult		13
fat body, larvae L3 wandering		1
fat body, white prepupae		14
fat body, pupae P8		21
carcass, larvae L3 wandering		36
carcass, 1-day adult		22
carcass, 4-day adult		18
carcass, 20-day adult		15
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		17
accessory gland, mated 4-day male		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		4
central nervous system, pupae P8		5
head, virgin 1-day female		22
head, virgin 4-day female		6
head, virgin 20-day female		10
head, mated 1-day female		9
head, mated 4-day female		22
head, mated 20-day female		7
head, mated 1-day male		9
head, mated 4-day male		23
head, mated 20-day male		12
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		18
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		19
digestive system, 4-day adult		14
digestive system, 20-day adult		13
fat body, larvae L3 wandering		1
fat body, white prepupae		14
fat body, pupae P8		21
carcass, larvae L3 wandering		36
carcass, 1-day adult		22
carcass, 4-day adult		18
carcass, 20-day adult		15
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		17
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		4
central nervous system, pupae P8		5
head, virgin 1-day female		22
head, virgin 4-day female		6
head, virgin 20-day female		10
head, mated 1-day female		9
head, mated 4-day female		22
head, mated 20-day female		7
head, mated 1-day male		9
head, mated 4-day male		23
head, mated 20-day male		12
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		18
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		19
digestive system, 4-day adult		14
digestive system, 20-day adult		13
fat body, larvae L3 wandering		1
fat body, white prepupae		14
fat body, pupae P8		21
carcass, larvae L3 wandering		(36)
carcass, 1-day adult		22
carcass, 4-day adult		18
carcass, 20-day adult		15
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		17
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		4
central nervous system, pupae P8		5
head, virgin 1-day female		22
head, virgin 4-day female		6
head, virgin 20-day female		10
head, mated 1-day female		9
head, mated 4-day female		22
head, mated 20-day female		7
head, mated 1-day male		9
head, mated 4-day male		23
head, mated 20-day male		12
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		18
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		19
digestive system, 4-day adult		14
digestive system, 20-day adult		13
fat body, larvae L3 wandering		1
fat body, white prepupae		14
fat body, pupae P8		21
carcass, larvae L3 wandering		36
carcass, 1-day adult		22
carcass, 4-day adult		18
carcass, 20-day adult		15
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		17
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		8
central nervous system, larvae L3		4
central nervous system, pupae P8		5
head, virgin 1-day female		22
head, virgin 4-day female		6
head, virgin 20-day female		10
head, mated 1-day female		9
head, mated 4-day female		22
head, mated 20-day female		7
head, mated 1-day male		9
head, mated 4-day male		23
head, mated 20-day male		12
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		18
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		19
digestive system, 4-day adult		14
digestive system, 20-day adult		13
fat body, larvae L3 wandering		1
fat body, white prepupae		14
fat body, pupae P8		21
carcass, larvae L3 wandering		36
carcass, 1-day adult		22
carcass, 4-day adult		18
carcass, 20-day adult		15
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		17
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during early pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		12
embryo 02-04hr		25
embryo 04-06hr		36
embryo 06-08hr		55
embryo 08-10hr		53
embryo 10-12hr		83
embryo 12-14hr		60
embryo 14-16hr		42
embryo 16-18hr		24
embryo 18-20hr		10
embryo 20-22hr		8
embryo 22-24hr		10
larva L1		8
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		36
white prepupae new		54
white prepupae 12hr		130
white prepupae 24hr		136
pupae 2d postWPP		71
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		29
adult male 05day		20
adult male 30day		16
adult female 01day		17
adult female 05day		12
adult female 30day		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		12
embryo 02-04hr		25
embryo 04-06hr		(36)
embryo 06-08hr		(55)
embryo 08-10hr		(53)
embryo 10-12hr		(83)
embryo 12-14hr		(60)
embryo 14-16hr		(42)
embryo 16-18hr		24
embryo 18-20hr		10
embryo 20-22hr		8
embryo 22-24hr		10
larva L1		8
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		(36)
white prepupae new		(54)
white prepupae 12hr		(130)
white prepupae 24hr		(136)
pupae 2d postWPP		(71)
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		(29)
adult male 05day		20
adult male 30day		16
adult female 01day		17
adult female 05day		12
adult female 30day		14
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		12
embryo 02-04hr		25
embryo 04-06hr		36
embryo 06-08hr		55
embryo 08-10hr		53
embryo 10-12hr		83
embryo 12-14hr		60
embryo 14-16hr		42
embryo 16-18hr		24
embryo 18-20hr		10
embryo 20-22hr		8
embryo 22-24hr		10
larva L1		8
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		36
white prepupae new		54
white prepupae 12hr		(130)
white prepupae 24hr		(136)
pupae 2d postWPP		71
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		29
adult male 05day		20
adult male 30day		16
adult female 01day		17
adult female 05day		12
adult female 30day		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		12
embryo 02-04hr		25
embryo 04-06hr		36
embryo 06-08hr		55
embryo 08-10hr		53
embryo 10-12hr		83
embryo 12-14hr		60
embryo 14-16hr		42
embryo 16-18hr		24
embryo 18-20hr		10
embryo 20-22hr		8
embryo 22-24hr		10
larva L1		8
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		36
white prepupae new		54
white prepupae 12hr		130
white prepupae 24hr		136
pupae 2d postWPP		71
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		29
adult male 05day		20
adult male 30day		16
adult female 01day		17
adult female 05day		12
adult female 30day		14
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		12
embryo 02-04hr		25
embryo 04-06hr		36
embryo 06-08hr		55
embryo 08-10hr		53
embryo 10-12hr		83
embryo 12-14hr		60
embryo 14-16hr		42
embryo 16-18hr		24
embryo 18-20hr		10
embryo 20-22hr		8
embryo 22-24hr		10
larva L1		8
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		36
white prepupae new		54
white prepupae 12hr		130
white prepupae 24hr		136
pupae 2d postWPP		71
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		29
adult male 05day		20
adult male 30day		16
adult female 01day		17
adult female 05day		12
adult female 30day		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		12
embryo 02-04hr		25
embryo 04-06hr		(36)
embryo 06-08hr		(55)
embryo 08-10hr		(53)
embryo 10-12hr		(83)
embryo 12-14hr		(60)
embryo 14-16hr		(42)
embryo 16-18hr		24
embryo 18-20hr		10
embryo 20-22hr		8
embryo 22-24hr		10
larva L1		8
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		(36)
white prepupae new		(54)
white prepupae 12hr		(130)
white prepupae 24hr		(136)
pupae 2d postWPP		(71)
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		29
adult male 05day		20
adult male 30day		16
adult female 01day		17
adult female 05day		12
adult female 30day		14
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		12
embryo 02-04hr		25
embryo 04-06hr		36
embryo 06-08hr		55
embryo 08-10hr		53
embryo 10-12hr		83
embryo 12-14hr		60
embryo 14-16hr		42
embryo 16-18hr		24
embryo 18-20hr		10
embryo 20-22hr		8
embryo 22-24hr		10
larva L1		8
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		36
white prepupae new		54
white prepupae 12hr		130
white prepupae 24hr		136
pupae 2d postWPP		71
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		29
adult male 05day		20
adult male 30day		16
adult female 01day		17
adult female 05day		12
adult female 30day		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		12
embryo 02-04hr		25
embryo 04-06hr		36
embryo 06-08hr		55
embryo 08-10hr		53
embryo 10-12hr		83
embryo 12-14hr		60
embryo 14-16hr		42
embryo 16-18hr		24
embryo 18-20hr		10
embryo 20-22hr		8
embryo 22-24hr		10
larva L1		8
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		36
white prepupae new		54
white prepupae 12hr		130
white prepupae 24hr		136
pupae 2d postWPP		71
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		29
adult male 05day		20
adult male 30day		16
adult female 01day		17
adult female 05day		12
adult female 30day		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		4
Schneider line 2 Sg4		8
embryonic 1182-4H		2
embryonic GM2		4
embryonic Kc167		11
embryonic S1		1
embryonic S3		4
leg disc CME L1		5
wing disc CME-W2		3
wing disc ML-DmD8		3
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		4
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		4
eye-antennal disc ML-DmD11		4
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		2
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		3
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		2
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		4
Schneider line 2 Sg4		8
embryonic 1182-4H		2
embryonic GM2		4
embryonic Kc167		11
embryonic S1		1
embryonic S3		4
leg disc CME L1		5
wing disc CME-W2		3
wing disc ML-DmD8		3
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		4
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		4
eye-antennal disc ML-DmD11		4
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		2
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		3
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		4
Schneider line 2 Sg4		8
embryonic 1182-4H		2
embryonic GM2		4
embryonic Kc167		11
embryonic S1		1
embryonic S3		4
leg disc CME L1		5
wing disc CME-W2		3
wing disc ML-DmD8		3
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		4
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		4
eye-antennal disc ML-DmD11		4
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		2
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		3
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		4
Schneider line 2 Sg4		8
embryonic 1182-4H		2
embryonic GM2		4
embryonic Kc167		11
embryonic S1		1
embryonic S3		4
leg disc CME L1		5
wing disc CME-W2		3
wing disc ML-DmD8		3
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		4
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		4
eye-antennal disc ML-DmD11		4
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		2
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		3
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		4
Schneider line 2 Sg4		8
embryonic 1182-4H		2
embryonic GM2		4
embryonic Kc167		11
embryonic S1		1
embryonic S3		4
leg disc CME L1		5
wing disc CME-W2		3
wing disc ML-DmD8		3
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		4
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		4
eye-antennal disc ML-DmD11		4
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		2
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		3
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		2
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		4
Schneider line 2 Sg4		8
embryonic 1182-4H		2
embryonic GM2		4
embryonic Kc167		11
embryonic S1		1
embryonic S3		4
leg disc CME L1		5
wing disc CME-W2		3
wing disc ML-DmD8		3
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		4
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		4
eye-antennal disc ML-DmD11		4
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		2
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		3
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		4
Schneider line 2 Sg4		8
embryonic 1182-4H		2
embryonic GM2		4
embryonic Kc167		11
embryonic S1		1
embryonic S3		4
leg disc CME L1		5
wing disc CME-W2		3
wing disc ML-DmD8		3
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		4
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		4
eye-antennal disc ML-DmD11		4
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		2
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		3
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		4
Schneider line 2 Sg4		8
embryonic 1182-4H		2
embryonic GM2		4
embryonic Kc167		11
embryonic S1		1
embryonic S3		4
leg disc CME L1		5
wing disc CME-W2		3
wing disc ML-DmD8		3
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		4
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		4
eye-antennal disc ML-DmD11		4
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		2
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		3
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		4
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		3
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		5
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		4
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		4
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		3
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		5
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		4
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		4
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		3
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		5
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		4
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		4
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		3
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		5
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		4
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		4
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		3
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		5
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		4
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		4
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		3
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		5
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		4
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		4
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		3
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		5
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		4
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		4
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		4
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		3
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		5
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		4
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_25

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0036549

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG10516-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

CG10516^EY13059

Sterility

Allele

fertile

CG10516^EY13059

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of CG10516	Class	Mutagen	Stocks	Known lesion
CG10516^EY13059		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG10516	Class	Mutagen	Stocks	Known lesion
CG10516^GD6122		in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD6122}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}CG10516^EY13059

Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )

There is no information supporting a biological process, cellular component or molecular function term for this gene.

(Based on a review of available data on: 2010-10-21)

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 0 terms )

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG10516 allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0036549

Orthologs

OrthoDB Orthologs (42) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6GMVH0

Dipteran inclusive ortholog search

EOG6PCB1S

Insect inclusive ortholog search

EOG6BK40Q

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

EOG6WHNDR

Orthologs in Drosophila Species (EOG6GMVH0)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA28209

Drosophila persimilis

Dper\GL12742

Drosophila persimilis

Dper\GL12741

Drosophila willistoni

Dwil\GK24452

Drosophila virilis

Dvir\GJ13571

Drosophila mojavensis

Dmoj\GI11875

Drosophila grimshawi

Dgri\GH14773

Orthologs in non-Drosophila Dipterans (EOG6PCB1S)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002607

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018604

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018607

Orthologs in non-Dipteran Insects (EOG6BK40Q)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB44654

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG018567

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH18419

Atta cephalotes

Leafcutter ant

Acep\ACEP10525

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO22536

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL13962

Linepithema humile

Argentine ant

Lhum\LH17868

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB22823

Solenopsis invicta

Red fire ant

Sinv\SINV20684

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001478

Bombyx mori

Silkmoth

Bmor\BGIBMGA008564

Pediculus humanus

Human body louse

Phum\PHUM011770

Tribolium castaneum

Red flour beetle

Tcas\TC006623

Orthologs in non-Insect Arthropods (None identified)

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (EOG6WHNDR)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_001318gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_001319gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_023964gn

Danio rerio

Zebrafish

Drer\LOC559863

Danio rerio

Zebrafish

Drer\CU929145.4

Xenopus tropicalis

Western clawed frog

Xtro\spsb3

Gallus gallus

Domestic chicken

Ggal\SPSB3

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000043298

Rattus norvegicus

Norway rat

Rnor\Spsb3

Homo sapiens

Human

Hsap\SPSB3

Homo sapiens

Human

Hsap\ENSG00000261661

Human Orthologs (2)

Gene

OMIM

HGNC

Hsap\SPSB3

Hsap\ENSG00000261661

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA28209

Drosophila willistoni

Dwil\GK24452

Drosophila virilis

Dvir\GJ13571

Drosophila mojavensis

Dmoj\GI11875

Drosophila grimshawi

Dgri\GH14773

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

Bloomington

21405

y¹ w^67c23; P{EPgy2}CG10516^EY13059

Genomic Clones ( 1 )

BACR18K24

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 51 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

AT20211

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0036549

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

39738

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_140562

RefSeq (Proteins)

•

NP_648819

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

39738

UniProtKB/TrEMBL

•

Q9VUW0

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

B30.2/SPRY domain (IPR001870)

SPla/RYanodine receptor SPRY (IPR003877)

Concanavalin A-like lectin/glucanases superfamily (IPR008985)

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0036549

DRSC - Results from RNAi screens.

•

FBgn0036549

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0036549

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG10516-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0036549

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

39738

modMine - Data generated by the modENCODE project.

•

FBgn0036549

Synonyms & Secondary IDs ( 1 )

Reported As

Symbol Synonym

CG10516

(Cooper and Kennison, 2011)

Name Synonym

Secondary FlyBase IDs

References ( 10 )

Research paper

Cooper and Kennison, 2011, PLoS ONE 6(8): e23509: Molecular Genetic Analyses of Polytene Chromosome Region 72A-D in Drosophila melanogaster Reveal a Gene Desert in 72D. [FBrf0214748]

Supplementary material

The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Dietzl et al., 2007, Nature 448(7150):: Supplementary Table 1. [FBrf0202579]

Personal communication to FlyBase

Gene Disruption Project members, 2001-, (Computer file): (Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

FlyBase analysis

FlyBase Curators, 2010-, Gene Ontology (GO) annotation review.: Gene Ontology (GO) annotation review. [FBrf0216433]
FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase.: Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398]
FlyBase, 1992-, FlyBase curation.: FlyBase curation. [FBrf0105495]

Curated genome annotation

FamiliarityBreedsContempt, 1999.11, [Automatic annotations performed at the Celera Jamboreee.]: [Automatic annotations performed at the Celera Jamboreee.] [FBrf0126705]