FlyBase Gene Report: Dmel\Cpr72Ec

CPR CUTICLE PROTEIN FAMILY -: The largest cuticle protein family, the CPR family, contain a 35 amino acid R&R consensus (named after Rebers and Riddiford, PMID:2462055), which in an extended form has been shown to bind chitin (PMID:15475300). (Adapted from FBrf0242484.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Cpr72Ec or the JBrowse view of Dmel\Cpr72Ec for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Cpr72Ec-RA

FBtr0075424

NM_140627

1447

429

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Cpr72Ec-PA

FBpp0075182

50.6

429

5.15

NP_648884

AAF49476

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VV46

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cpr72Ec using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cpr72Ec_1

Linkouts

Gene Ontology (7 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

structural constituent of eye lens

inferred from direct assay

(Stahl et al., 2017)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

structural constituent of chitin-based larval cuticle

inferred from sequence model

(Karouzou et al., 2007)

structural constituent of cuticle

inferred from electronic annotation with InterPro:IPR000618

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

chitin-based cuticle development

inferred from sequence model

(Karouzou et al., 2007)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

chitin-based extracellular matrix

inferred from sequence model

(Karouzou et al., 2007)

extracellular matrix

inferred from biological aspect of ancestor with PANTHER:PTN000955699

(assigned by GO_Central )

(Gaudet et al., 2011)

extracellular space

inferred from sequence model

(Liu et al., 2006)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

pharate adult stage P8

interommatidial cell

(Stahl et al., 2017)

pigment cell

(Stahl et al., 2017)

cone cell

(Stahl et al., 2017)

photoreceptor cell

(Stahl et al., 2017)

Additional Descriptive Data

Cpr72Ec is expressed in all cell types of the eye, with particularly high expression in higher order interommatidial pigment cells, and weaker expression in cone cells, primary pigment cells and photoreceptors. It's expression is eye-specific.

(Stahl et al., 2017)

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Cpr72Ec in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cpr72Ec

View exonic expression by tissue for Dmel\Cpr72Ec

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036619

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0036619

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG4784-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4784

Flygut - An atlas of the Drosophila adult midgut

FBgn0036619

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cpr72Ec

Transgenic constructs containing regulatory region of Cpr72Ec

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Cpr72Ec^GD14888

viable, with Scer\GAL4^pnr-MD237

Cpr72Ec^GD14888

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (0)

No records found.

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (0)

No records found.

Rattus norvegicus (Norway rat) (0)

No records found.

Xenopus tropicalis (Western clawed frog) (0)

No records found.

Danio rerio (Zebrafish) (0)

No records found.

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190F2S )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Cpr72Ec

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00010712

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18429

Drosophila persimilis

Dper\GL18001

Drosophila willistoni

Dwil\GK20818

Drosophila virilis

Dvir\GJ12623

Drosophila mojavensis

Dmoj\GI16874

Drosophila grimshawi

Dgri\GH15596

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150AGN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17022300

Glossina morsitans

Tsetse fly

Gmor\GMOY010403

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09530

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0BVN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_08400

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0B98 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Black-legged tick

Isca\ISCW019781

Ixodes scapularis

Black-legged tick

Isca\ISCW016310

Stegodyphus mimosarum

African social velvet spider

Smim\KFM73244.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM73249.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur32g02090

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_246873

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_303722

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (43)

Gene Symbol

Score

Source

Alignment

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0036619

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

39816

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-44

Cytogenetic map

72E2-72E2

Sequence location

3L:16,365,894..16,367,340 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

72E2-72E2

Limits computationally determined from genome sequence between P{lacW}l(3)s3123^s3123 and P{lacW}l(3)j10E8^j10E8&P{PZ}l(3)10532¹⁰⁵³²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-44

(FlyBase, 2016)

3-38.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Bloomington

43625

y¹ w^*; Mi{MIC}Cpr72Ec^MI07254 Mi{MIC}MI07254

VDRC

v29452

w¹¹¹⁸ P{GD14888}v29452

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (15)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

GH03623

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0036619

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0036619

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39816

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Cpr72Ec CG4784

(Willis, 2007.1.3)

Source for database merge of

Additional comments

Other Comments

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.

(Claridge-Chang et al., 2001)

Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.

(McDonald and Rosbash, 2001)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 26 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39816

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VV46

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036619

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0036619

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cpr72Ec_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4784

Flygut - An atlas of the Drosophila adult midgut

FBgn0036619

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39816

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4784

modMine - A data warehouse for the modENCODE project

FBgn0036619

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0036619

DRSC - Results frm RNAi screens

FBgn0036619

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0036619

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG4784-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0036619

FlyMine - An integrated database for Drosophila genomics

FBgn0036619

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

39816

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG4784

(Çiçek et al., 2016, Palu and Thummel, 2016, Rosa-Ferreira et al., 2015, Jensen et al., 2013, Rodriguez et al., 2013, Cao et al., 2011, Robinson et al., 2010, Han et al., 2006, Liu et al., 2006, Wijnen et al., 2006, Yang et al., 2005)

CT15377

(Ceriani et al., 2002)

Cpr72Ec

(Stahl et al., 2017, Hosamani et al., 2016, Kang et al., 2016, Ma et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Napoletano et al., 2011, Cornman, 2009, Karouzou et al., 2007, Karouzou et al., 2007, Willis, 2007.1.3)

Name Synonyms

CG4784

(Yang et al., 2005)

Cuticular protein 72Ec

(Kim et al., 2015)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (47)

Report Sections

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