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Gene Dmel\TSG101

General Information
Symbol	Dmel\TSG101	Species	D. melanogaster
Name	tumor suppressor protein 101	Annotation symbol	CG9712
Feature type	protein_coding_gene	FlyBase ID	FBgn0036666
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	ept, dTsg101, vps23
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	73D1-73D1	Sequence location	3L:16,835,394..16,837,685 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene tumor suppressor protein 101 is referred to in FlyBase by the symbol Dmel\TSG101 (CG9712, FBgn0036666). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: negative regulation of growth; trachea morphogenesis; terminal branching, open tracheal system; negative regulation of growth of symbiont in host; regulation of JAK-STAT cascade; negative regulation of imaginal disc growth; maintenance of apical/basal cell polarity. 12 alleles are reported. The phenotypes of these alleles are annotated with: yolk granule; oocyte; embryonic/larval tracheal system; terminal tracheal cell; vesicle. It has one annotated transcript and one annotated polypeptide. Protein features are: Steadiness box; Ubiquitin E2 variant, N-terminal; Ubiquitin-conjugating enzyme/RWD-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed during late larval stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval/adult Malpighian tubules, adult heart, larval fat body, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult brain, adult crop, larval/adult midgut, larval/adult hindgut, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1640247_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of TSG101. Gene sequence location is 3L:16835394..16837685.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 73D1-73D1   Limits computationally determined from genome sequence between P{PZ}Baldspot02281 and P{PZ}blot01658
Experimentally Determined Cytological Location
Cytogenetic map Notes References 73D1-73D1   Location based on deficiency mapping with Df(3L)Exel9002. Limits computationally determined from genome sequence between P{PZ}Baldspot02281 and P{PZ}blot01658   (Ghabrial et al., 2011)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\TSG101 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) TSG101-RA FBtr0075329  NM_079396   1757   408
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein TSG101-PA   FBpp0075088   45.2   408   6.32     NP_524120   AAF49406
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ubiquitin E2 variant, N-terminal (IPR008883) Ubiquitin-conjugating enzyme/RWD-like (IPR016135) Steadiness box (IPR017916)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA951728   AE014296 AAF49406   AF315343 AAG29564   AI110074   AI297906   AI387669   AI403122   AI515482   AI944688   AY122227 AAM52739   BF486224   BF488098   BF502840   BG636513   BI229055   BI240326   BI368647   BI566952   BI571503   BI579556   BI585542   BI588356   BI588753   BI593066   BI613318   BI637764   BP544110   CA807819   CO153278   CO153913   CO178344   CO192058   CO263955   CO276955   CO338647   CZ483456   CZ483457   DQ017452 AAY82230   DQ017453 AAY82231   EC063838   EC083746   EC085243   EC195115   EC195808   EC216514   EC234253   EC240903   EC265442   EC267336   GH748136   GH782419   GH787311   GH788599   GH807346   GH812159   GH824827   GH829677   GH836828   GH843025   GH860815   GH861137   GH866607   GH885198   GH891273   GH901995   GH910839   GH916309   GH917339   GH937651   GH940516
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VVA7 Q8MQZ0 Q3YNA6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   garland cell primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval garland cell   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval/adult Malpighian tubules, adult heart, larval fat body, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult brain, adult crop, larval/adult midgut, larval/adult hindgut, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    503.35 Larval Midgut    341.3 Larval Hindgut    435.4 Larval Malpighian Tubules    935.2 Larval Fat Body    545.4 Larval Salivary Gland    339.7 Larval Trachea    471.05 Larval Carcass    278.375 Adult Head    340.6 Adult Eye    634.275 Adult Brain    480.5 Adult Thoracic-Abdominal Ganglion    559.4 Adult Crop    372.5 Adult Midgut    309.4 Adult Hindgut    324.8 Adult Malpighian Tubules    769.3 Adult Fat Body    234 Adult Salivary Gland    273 Adult Heart    550.7 Adult VirginFemale Spermatheca    220.6 Adult InseminatedFemale Spermatheca    199.1 Adult Ovary    274.5 Adult Testis    1428.7 Adult Male Accessory Gland    186.3 Adult Carcass    321 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  503.35 Larval Midgut    341.3 Larval Hindgut    435.4 Larval Malpighian Tubules  (935.2) Larval Fat Body  (545.4) Larval Salivary Gland    339.7 Larval Trachea    471.05 Larval Carcass    278.375 Adult Head    340.6 Adult Eye  (634.275) Adult Brain    480.5 Adult Thoracic-Abdominal Ganglion  (559.4) Adult Crop    372.5 Adult Midgut    309.4 Adult Hindgut    324.8 Adult Malpighian Tubules  (769.3) Adult Fat Body    234 Adult Salivary Gland    273 Adult Heart  (550.7) Adult VirginFemale Spermatheca    220.6 Adult InseminatedFemale Spermatheca    199.1 Adult Ovary    274.5 Adult Testis  (1428.7) Adult Male Accessory Gland    186.3 Adult Carcass    321 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    503.35 Larval Midgut    341.3 Larval Hindgut    435.4 Larval Malpighian Tubules    935.2 Larval Fat Body    545.4 Larval Salivary Gland    339.7 Larval Trachea    471.05 Larval Carcass    278.375 Adult Head    340.6 Adult Eye    634.275 Adult Brain    480.5 Adult Thoracic-Abdominal Ganglion    559.4 Adult Crop    372.5 Adult Midgut    309.4 Adult Hindgut    324.8 Adult Malpighian Tubules    769.3 Adult Fat Body    234 Adult Salivary Gland    273 Adult Heart    550.7 Adult VirginFemale Spermatheca    220.6 Adult InseminatedFemale Spermatheca    199.1 Adult Ovary    274.5 Adult Testis  (1428.7) Adult Male Accessory Gland    186.3 Adult Carcass    321 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    503.35 Larval Midgut    341.3 Larval Hindgut    435.4 Larval Malpighian Tubules    935.2 Larval Fat Body    545.4 Larval Salivary Gland    339.7 Larval Trachea    471.05 Larval Carcass    278.375 Adult Head    340.6 Adult Eye    634.275 Adult Brain    480.5 Adult Thoracic-Abdominal Ganglion    559.4 Adult Crop    372.5 Adult Midgut    309.4 Adult Hindgut    324.8 Adult Malpighian Tubules    769.3 Adult Fat Body    234 Adult Salivary Gland    273 Adult Heart    550.7 Adult VirginFemale Spermatheca    220.6 Adult InseminatedFemale Spermatheca    199.1 Adult Ovary    274.5 Adult Testis    1428.7 Adult Male Accessory Gland    186.3 Adult Carcass    321 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    503.35 Larval Midgut    341.3 Larval Hindgut    435.4 Larval Malpighian Tubules    935.2 Larval Fat Body    545.4 Larval Salivary Gland    339.7 Larval Trachea    471.05 Larval Carcass    278.375 Adult Head    340.6 Adult Eye    634.275 Adult Brain    480.5 Adult Thoracic-Abdominal Ganglion    559.4 Adult Crop    372.5 Adult Midgut    309.4 Adult Hindgut    324.8 Adult Malpighian Tubules    769.3 Adult Fat Body    234 Adult Salivary Gland    273 Adult Heart    550.7 Adult VirginFemale Spermatheca    220.6 Adult InseminatedFemale Spermatheca    199.1 Adult Ovary    274.5 Adult Testis    1428.7 Adult Male Accessory Gland    186.3 Adult Carcass    321 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  503.35 Larval Midgut    341.3 Larval Hindgut    435.4 Larval Malpighian Tubules  (935.2) Larval Fat Body  545.4 Larval Salivary Gland    339.7 Larval Trachea    471.05 Larval Carcass    278.375 Adult Head    340.6 Adult Eye  634.275 Adult Brain    480.5 Adult Thoracic-Abdominal Ganglion  559.4 Adult Crop    372.5 Adult Midgut    309.4 Adult Hindgut    324.8 Adult Malpighian Tubules  769.3 Adult Fat Body    234 Adult Salivary Gland    273 Adult Heart  550.7 Adult VirginFemale Spermatheca    220.6 Adult InseminatedFemale Spermatheca    199.1 Adult Ovary    274.5 Adult Testis  (1428.7) Adult Male Accessory Gland    186.3 Adult Carcass    321 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    503.35 Larval Midgut    341.3 Larval Hindgut    435.4 Larval Malpighian Tubules    935.2 Larval Fat Body    545.4 Larval Salivary Gland    339.7 Larval Trachea    471.05 Larval Carcass    278.375 Adult Head    340.6 Adult Eye    634.275 Adult Brain    480.5 Adult Thoracic-Abdominal Ganglion    559.4 Adult Crop    372.5 Adult Midgut    309.4 Adult Hindgut    324.8 Adult Malpighian Tubules    769.3 Adult Fat Body    234 Adult Salivary Gland    273 Adult Heart    550.7 Adult VirginFemale Spermatheca    220.6 Adult InseminatedFemale Spermatheca    199.1 Adult Ovary    274.5 Adult Testis  1428.7 Adult Male Accessory Gland    186.3 Adult Carcass    321 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    503.35 Larval Midgut    341.3 Larval Hindgut    435.4 Larval Malpighian Tubules    935.2 Larval Fat Body    545.4 Larval Salivary Gland    339.7 Larval Trachea    471.05 Larval Carcass    278.375 Adult Head    340.6 Adult Eye    634.275 Adult Brain    480.5 Adult Thoracic-Abdominal Ganglion    559.4 Adult Crop    372.5 Adult Midgut    309.4 Adult Hindgut    324.8 Adult Malpighian Tubules    769.3 Adult Fat Body    234 Adult Salivary Gland    273 Adult Heart    550.7 Adult VirginFemale Spermatheca    220.6 Adult InseminatedFemale Spermatheca    199.1 Adult Ovary    274.5 Adult Testis    1428.7 Adult Male Accessory Gland    186.3 Adult Carcass    321 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    340.6   NA  Eye    634.275   NA  Brain    480.5   503.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    559.4   NA  Crop    372.5   341.3  Midgut    309.4   435.4  Hindgut    324.8   935.2  Malpighian Tubules    769.3   545.4  Fat Body    234   339.7  Salivary Gland    273   NA  Heart    550.7   471.05  Trachea    NA   NA  VirginFemale Spermatheca    220.6   NA  InseminatedFemale Spermatheca    199.1   NA  Ovary    274.5   NA  Testis    1428.7   NA  Male Accessory Gland    186.3   278.375  Carcass    321 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    129 central nervous system, larvae L3    54 central nervous system, pupae P8    39 head, virgin 1-day female    34 head, virgin 4-day female    35 head, virgin 20-day female    46 head, mated 1-day female    29 head, mated 4-day female    36 head, mated 20-day female    37 head, mated 1-day male    47 head, mated 4-day male    52 head, mated 20-day male    77 salivary gland, larvae L3 wandering    19 salivary gland, white prepupae    54 digestive system, larvae L3 wandering    104 digestive system, 1-day adult    61 digestive system, 4-day adult    47 digestive system, 20-day adult    61 fat body, larvae L3 wandering    22 fat body, white prepupae    61 fat body, pupae P8    50 carcass, larvae L3 wandering    62 carcass, 1-day adult    34 carcass, 4-day adult    32 carcass, 20-day adult    43 ovary, virgin 4-day female    50 ovary, mated 4-day female    40 testis, mated 4-day male    289 accessory gland, mated 4-day male    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (129) central nervous system, larvae L3  (54) central nervous system, pupae P8  (39) head, virgin 1-day female  (34) head, virgin 4-day female  (35) head, virgin 20-day female  (46) head, mated 1-day female  (29) head, mated 4-day female  (36) head, mated 20-day female  (37) head, mated 1-day male  (47) head, mated 4-day male  (52) head, mated 20-day male  (77) salivary gland, larvae L3 wandering    19 salivary gland, white prepupae  (54) digestive system, larvae L3 wandering  (104) digestive system, 1-day adult  (61) digestive system, 4-day adult  (47) digestive system, 20-day adult  (61) fat body, larvae L3 wandering    22 fat body, white prepupae  (61) fat body, pupae P8  (50) carcass, larvae L3 wandering  (62) carcass, 1-day adult  (34) carcass, 4-day adult  (32) carcass, 20-day adult  (43) ovary, virgin 4-day female  (50) ovary, mated 4-day female  (40) testis, mated 4-day male  (289) accessory gland, mated 4-day male  (39) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (129) central nervous system, larvae L3    54 central nervous system, pupae P8    39 head, virgin 1-day female    34 head, virgin 4-day female    35 head, virgin 20-day female    46 head, mated 1-day female    29 head, mated 4-day female    36 head, mated 20-day female    37 head, mated 1-day male    47 head, mated 4-day male    52 head, mated 20-day male    77 salivary gland, larvae L3 wandering    19 salivary gland, white prepupae    54 digestive system, larvae L3 wandering  104 digestive system, 1-day adult    61 digestive system, 4-day adult    47 digestive system, 20-day adult    61 fat body, larvae L3 wandering    22 fat body, white prepupae    61 fat body, pupae P8    50 carcass, larvae L3 wandering    62 carcass, 1-day adult    34 carcass, 4-day adult    32 carcass, 20-day adult    43 ovary, virgin 4-day female    50 ovary, mated 4-day female    40 testis, mated 4-day male  (289) accessory gland, mated 4-day male    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    129 central nervous system, larvae L3    54 central nervous system, pupae P8    39 head, virgin 1-day female    34 head, virgin 4-day female    35 head, virgin 20-day female    46 head, mated 1-day female    29 head, mated 4-day female    36 head, mated 20-day female    37 head, mated 1-day male    47 head, mated 4-day male    52 head, mated 20-day male    77 salivary gland, larvae L3 wandering    19 salivary gland, white prepupae    54 digestive system, larvae L3 wandering    104 digestive system, 1-day adult    61 digestive system, 4-day adult    47 digestive system, 20-day adult    61 fat body, larvae L3 wandering    22 fat body, white prepupae    61 fat body, pupae P8    50 carcass, larvae L3 wandering    62 carcass, 1-day adult    34 carcass, 4-day adult    32 carcass, 20-day adult    43 ovary, virgin 4-day female    50 ovary, mated 4-day female    40 testis, mated 4-day male    289 accessory gland, mated 4-day male    39 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    129 central nervous system, larvae L3    54 central nervous system, pupae P8    39 head, virgin 1-day female    34 head, virgin 4-day female    35 head, virgin 20-day female    46 head, mated 1-day female    29 head, mated 4-day female    36 head, mated 20-day female    37 head, mated 1-day male    47 head, mated 4-day male    52 head, mated 20-day male    77 salivary gland, larvae L3 wandering    19 salivary gland, white prepupae    54 digestive system, larvae L3 wandering    104 digestive system, 1-day adult    61 digestive system, 4-day adult    47 digestive system, 20-day adult    61 fat body, larvae L3 wandering    22 fat body, white prepupae    61 fat body, pupae P8    50 carcass, larvae L3 wandering    62 carcass, 1-day adult    34 carcass, 4-day adult    32 carcass, 20-day adult    43 ovary, virgin 4-day female    50 ovary, mated 4-day female    40 testis, mated 4-day male    289 accessory gland, mated 4-day male    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (129) central nervous system, larvae L3  (54) central nervous system, pupae P8  (39) head, virgin 1-day female  (34) head, virgin 4-day female  (35) head, virgin 20-day female  (46) head, mated 1-day female  29 head, mated 4-day female  (36) head, mated 20-day female  (37) head, mated 1-day male  (47) head, mated 4-day male  (52) head, mated 20-day male  (77) salivary gland, larvae L3 wandering    19 salivary gland, white prepupae  (54) digestive system, larvae L3 wandering  (104) digestive system, 1-day adult  (61) digestive system, 4-day adult  (47) digestive system, 20-day adult  (61) fat body, larvae L3 wandering    22 fat body, white prepupae  (61) fat body, pupae P8  (50) carcass, larvae L3 wandering  (62) carcass, 1-day adult  (34) carcass, 4-day adult  32 carcass, 20-day adult  (43) ovary, virgin 4-day female  (50) ovary, mated 4-day female  (40) testis, mated 4-day male  (289) accessory gland, mated 4-day male  (39) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  129 central nervous system, larvae L3    54 central nervous system, pupae P8    39 head, virgin 1-day female    34 head, virgin 4-day female    35 head, virgin 20-day female    46 head, mated 1-day female    29 head, mated 4-day female    36 head, mated 20-day female    37 head, mated 1-day male    47 head, mated 4-day male    52 head, mated 20-day male    77 salivary gland, larvae L3 wandering    19 salivary gland, white prepupae    54 digestive system, larvae L3 wandering  104 digestive system, 1-day adult    61 digestive system, 4-day adult    47 digestive system, 20-day adult    61 fat body, larvae L3 wandering    22 fat body, white prepupae    61 fat body, pupae P8    50 carcass, larvae L3 wandering    62 carcass, 1-day adult    34 carcass, 4-day adult    32 carcass, 20-day adult    43 ovary, virgin 4-day female    50 ovary, mated 4-day female    40 testis, mated 4-day male  (289) accessory gland, mated 4-day male    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    129 central nervous system, larvae L3    54 central nervous system, pupae P8    39 head, virgin 1-day female    34 head, virgin 4-day female    35 head, virgin 20-day female    46 head, mated 1-day female    29 head, mated 4-day female    36 head, mated 20-day female    37 head, mated 1-day male    47 head, mated 4-day male    52 head, mated 20-day male    77 salivary gland, larvae L3 wandering    19 salivary gland, white prepupae    54 digestive system, larvae L3 wandering    104 digestive system, 1-day adult    61 digestive system, 4-day adult    47 digestive system, 20-day adult    61 fat body, larvae L3 wandering    22 fat body, white prepupae    61 fat body, pupae P8    50 carcass, larvae L3 wandering    62 carcass, 1-day adult    34 carcass, 4-day adult    32 carcass, 20-day adult    43 ovary, virgin 4-day female    50 ovary, mated 4-day female    40 testis, mated 4-day male    289 accessory gland, mated 4-day male    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed during late larval stages, at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    44 embryo 04-06hr    36 embryo 06-08hr    35 embryo 08-10hr    34 embryo 10-12hr    31 embryo 12-14hr    32 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    23 embryo 20-22hr    18 embryo 22-24hr    20 larva L1    19 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    60 white prepupae new    60 white prepupae 12hr    58 white prepupae 24hr    68 pupae 2d postWPP    75 pupae 3d postWPP    66 pupae 4d postWPP    50 adult male 01day    78 adult male 05day    99 adult male 30day    95 adult female 01day    25 adult female 05day    32 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (39) embryo 02-04hr  (44) embryo 04-06hr  (36) embryo 06-08hr  (35) embryo 08-10hr  (34) embryo 10-12hr  (31) embryo 12-14hr  (32) embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    23 embryo 20-22hr    18 embryo 22-24hr    20 larva L1    19 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2  (30) larva L3 puffstage 3-6  (41) larva L3 puffstage 7-9  (60) white prepupae new  (60) white prepupae 12hr  (58) white prepupae 24hr  (68) pupae 2d postWPP  (75) pupae 3d postWPP  (66) pupae 4d postWPP  (50) adult male 01day  (78) adult male 05day  (99) adult male 30day  (95) adult female 01day    25 adult female 05day  (32) adult female 30day  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    44 embryo 04-06hr    36 embryo 06-08hr    35 embryo 08-10hr    34 embryo 10-12hr    31 embryo 12-14hr    32 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    23 embryo 20-22hr    18 embryo 22-24hr    20 larva L1    19 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    60 white prepupae new    60 white prepupae 12hr    58 white prepupae 24hr    68 pupae 2d postWPP    75 pupae 3d postWPP    66 pupae 4d postWPP    50 adult male 01day    78 adult male 05day    99 adult male 30day    95 adult female 01day    25 adult female 05day    32 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    44 embryo 04-06hr    36 embryo 06-08hr    35 embryo 08-10hr    34 embryo 10-12hr    31 embryo 12-14hr    32 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    23 embryo 20-22hr    18 embryo 22-24hr    20 larva L1    19 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    60 white prepupae new    60 white prepupae 12hr    58 white prepupae 24hr    68 pupae 2d postWPP    75 pupae 3d postWPP    66 pupae 4d postWPP    50 adult male 01day    78 adult male 05day    99 adult male 30day    95 adult female 01day    25 adult female 05day    32 adult female 30day    33 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    44 embryo 04-06hr    36 embryo 06-08hr    35 embryo 08-10hr    34 embryo 10-12hr    31 embryo 12-14hr    32 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    23 embryo 20-22hr    18 embryo 22-24hr    20 larva L1    19 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    60 white prepupae new    60 white prepupae 12hr    58 white prepupae 24hr    68 pupae 2d postWPP    75 pupae 3d postWPP    66 pupae 4d postWPP    50 adult male 01day    78 adult male 05day    99 adult male 30day    95 adult female 01day    25 adult female 05day    32 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (39) embryo 02-04hr  (44) embryo 04-06hr  (36) embryo 06-08hr  (35) embryo 08-10hr  (34) embryo 10-12hr  31 embryo 12-14hr  32 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    23 embryo 20-22hr    18 embryo 22-24hr    20 larva L1    19 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2  30 larva L3 puffstage 3-6  (41) larva L3 puffstage 7-9  (60) white prepupae new  (60) white prepupae 12hr  (58) white prepupae 24hr  (68) pupae 2d postWPP  (75) pupae 3d postWPP  (66) pupae 4d postWPP  (50) adult male 01day  (78) adult male 05day  (99) adult male 30day  (95) adult female 01day    25 adult female 05day  32 adult female 30day  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    44 embryo 04-06hr    36 embryo 06-08hr    35 embryo 08-10hr    34 embryo 10-12hr    31 embryo 12-14hr    32 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    23 embryo 20-22hr    18 embryo 22-24hr    20 larva L1    19 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    60 white prepupae new    60 white prepupae 12hr    58 white prepupae 24hr    68 pupae 2d postWPP    75 pupae 3d postWPP    66 pupae 4d postWPP    50 adult male 01day    78 adult male 05day    99 adult male 30day    95 adult female 01day    25 adult female 05day    32 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    44 embryo 04-06hr    36 embryo 06-08hr    35 embryo 08-10hr    34 embryo 10-12hr    31 embryo 12-14hr    32 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    23 embryo 20-22hr    18 embryo 22-24hr    20 larva L1    19 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    60 white prepupae new    60 white prepupae 12hr    58 white prepupae 24hr    68 pupae 2d postWPP    75 pupae 3d postWPP    66 pupae 4d postWPP    50 adult male 01day    78 adult male 05day    99 adult male 30day    95 adult female 01day    25 adult female 05day    32 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    199 Schneider line 2 Sg4    130 embryonic 1182-4H    152 embryonic GM2    114 embryonic Kc167    87 embryonic S1    207 embryonic S3    85 leg disc CME L1    148 wing disc CME-W2    112 wing disc ML-DmD8    105 wing disc ML-DmD9    189 wing disc ML-DmD16-c3    92 wing disc ML-DmD21    108 wing disc ML-DmD32    120 haltere disc ML-DmD17-c3    127 eye-antennal disc ML-DmD11    123 antennal disc ML-DmD20-c5    120 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    94 CNS ML-DmBG2-c2    132 tumorous blood cells mbn2    102 ovary fGS/OSS    53 ovary OSC    59 ovary OSS    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (199) Schneider line 2 Sg4  (130) embryonic 1182-4H  (152) embryonic GM2  (114) embryonic Kc167  (87) embryonic S1  (207) embryonic S3  (85) leg disc CME L1  (148) wing disc CME-W2  (112) wing disc ML-DmD8  (105) wing disc ML-DmD9  (189) wing disc ML-DmD16-c3  (92) wing disc ML-DmD21  (108) wing disc ML-DmD32  (120) haltere disc ML-DmD17-c3  (127) eye-antennal disc ML-DmD11  (123) antennal disc ML-DmD20-c5  (120) mixed discs ML-DmD4-c1  (110) CNS ML-DmBG1-c1  (94) CNS ML-DmBG2-c2  (132) tumorous blood cells mbn2  (102) ovary fGS/OSS  (53) ovary OSC  (59) ovary OSS  (41) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (199) Schneider line 2 Sg4  (130) embryonic 1182-4H  (152) embryonic GM2  (114) embryonic Kc167    87 embryonic S1  (207) embryonic S3    85 leg disc CME L1  (148) wing disc CME-W2  (112) wing disc ML-DmD8  105 wing disc ML-DmD9  (189) wing disc ML-DmD16-c3    92 wing disc ML-DmD21  (108) wing disc ML-DmD32  (120) haltere disc ML-DmD17-c3  (127) eye-antennal disc ML-DmD11  (123) antennal disc ML-DmD20-c5  (120) mixed discs ML-DmD4-c1  (110) CNS ML-DmBG1-c1    94 CNS ML-DmBG2-c2  (132) tumorous blood cells mbn2  102 ovary fGS/OSS    53 ovary OSC    59 ovary OSS    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    199 Schneider line 2 Sg4    130 embryonic 1182-4H    152 embryonic GM2    114 embryonic Kc167    87 embryonic S1    207 embryonic S3    85 leg disc CME L1    148 wing disc CME-W2    112 wing disc ML-DmD8    105 wing disc ML-DmD9    189 wing disc ML-DmD16-c3    92 wing disc ML-DmD21    108 wing disc ML-DmD32    120 haltere disc ML-DmD17-c3    127 eye-antennal disc ML-DmD11    123 antennal disc ML-DmD20-c5    120 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    94 CNS ML-DmBG2-c2    132 tumorous blood cells mbn2    102 ovary fGS/OSS    53 ovary OSC    59 ovary OSS    41 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    199 Schneider line 2 Sg4    130 embryonic 1182-4H    152 embryonic GM2    114 embryonic Kc167    87 embryonic S1    207 embryonic S3    85 leg disc CME L1    148 wing disc CME-W2    112 wing disc ML-DmD8    105 wing disc ML-DmD9    189 wing disc ML-DmD16-c3    92 wing disc ML-DmD21    108 wing disc ML-DmD32    120 haltere disc ML-DmD17-c3    127 eye-antennal disc ML-DmD11    123 antennal disc ML-DmD20-c5    120 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    94 CNS ML-DmBG2-c2    132 tumorous blood cells mbn2    102 ovary fGS/OSS    53 ovary OSC    59 ovary OSS    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (199) Schneider line 2 Sg4  (130) embryonic 1182-4H  (152) embryonic GM2  (114) embryonic Kc167  (87) embryonic S1  (207) embryonic S3  (85) leg disc CME L1  (148) wing disc CME-W2  (112) wing disc ML-DmD8  (105) wing disc ML-DmD9  (189) wing disc ML-DmD16-c3  (92) wing disc ML-DmD21  (108) wing disc ML-DmD32  (120) haltere disc ML-DmD17-c3  (127) eye-antennal disc ML-DmD11  (123) antennal disc ML-DmD20-c5  (120) mixed discs ML-DmD4-c1  (110) CNS ML-DmBG1-c1  (94) CNS ML-DmBG2-c2  (132) tumorous blood cells mbn2  (102) ovary fGS/OSS  (53) ovary OSC  (59) ovary OSS  (41) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (199) Schneider line 2 Sg4  130 embryonic 1182-4H  (152) embryonic GM2  114 embryonic Kc167    87 embryonic S1  (207) embryonic S3    85 leg disc CME L1  (148) wing disc CME-W2  112 wing disc ML-DmD8  105 wing disc ML-DmD9  (189) wing disc ML-DmD16-c3    92 wing disc ML-DmD21  108 wing disc ML-DmD32  120 haltere disc ML-DmD17-c3  127 eye-antennal disc ML-DmD11  123 antennal disc ML-DmD20-c5  120 mixed discs ML-DmD4-c1  110 CNS ML-DmBG1-c1    94 CNS ML-DmBG2-c2  132 tumorous blood cells mbn2  102 ovary fGS/OSS    53 ovary OSC    59 ovary OSS    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    199 Schneider line 2 Sg4    130 embryonic 1182-4H    152 embryonic GM2    114 embryonic Kc167    87 embryonic S1    207 embryonic S3    85 leg disc CME L1    148 wing disc CME-W2    112 wing disc ML-DmD8    105 wing disc ML-DmD9    189 wing disc ML-DmD16-c3    92 wing disc ML-DmD21    108 wing disc ML-DmD32    120 haltere disc ML-DmD17-c3    127 eye-antennal disc ML-DmD11    123 antennal disc ML-DmD20-c5    120 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    94 CNS ML-DmBG2-c2    132 tumorous blood cells mbn2    102 ovary fGS/OSS    53 ovary OSC    59 ovary OSS    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    27 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    43 Cadmium 50 mM 12 hrs, larvae L3    74 Cadmium 50 mM 48 hrs, 4-day adult    49 Cadmium 100 mM 48 hrs, 4-day adult    74 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    59 Ethanol 2.5% 3 hrs, larvae L3    72 Ethanol 5% 3 hrs, larvae L3    55 Ethanol 10% 3 hrs, larvae L3    49 Caffeine 1.5 mg/ml 4 hrs, larvae L3    47 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    65 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    79 Paraquat 10 mM 48 hrs, 4-day adult    97 Rotenone 2 μg 12 hrs, larvae L3    48 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (30) cold shock, 4-day adult  (27) heat shock, 4-day adult  (47) Cadmium 50 mM 6 hrs, larvae L3  (43) Cadmium 50 mM 12 hrs, larvae L3  (74) Cadmium 50 mM 48 hrs, 4-day adult  (49) Cadmium 100 mM 48 hrs, 4-day adult  (74) Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult  (44) Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult  (59) Ethanol 2.5% 3 hrs, larvae L3  (72) Ethanol 5% 3 hrs, larvae L3  (55) Ethanol 10% 3 hrs, larvae L3  (49) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (47) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (65) Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult  (79) Paraquat 10 mM 48 hrs, 4-day adult  (97) Rotenone 2 μg 12 hrs, larvae L3  (48) Rotenone 8 μg 12 hrs, larvae L3  (40) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    27 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    43 Cadmium 50 mM 12 hrs, larvae L3    74 Cadmium 50 mM 48 hrs, 4-day adult    49 Cadmium 100 mM 48 hrs, 4-day adult    74 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    59 Ethanol 2.5% 3 hrs, larvae L3    72 Ethanol 5% 3 hrs, larvae L3    55 Ethanol 10% 3 hrs, larvae L3    49 Caffeine 1.5 mg/ml 4 hrs, larvae L3    47 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    65 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    79 Paraquat 10 mM 48 hrs, 4-day adult    97 Rotenone 2 μg 12 hrs, larvae L3    48 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    27 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    43 Cadmium 50 mM 12 hrs, larvae L3    74 Cadmium 50 mM 48 hrs, 4-day adult    49 Cadmium 100 mM 48 hrs, 4-day adult    74 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    59 Ethanol 2.5% 3 hrs, larvae L3    72 Ethanol 5% 3 hrs, larvae L3    55 Ethanol 10% 3 hrs, larvae L3    49 Caffeine 1.5 mg/ml 4 hrs, larvae L3    47 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    65 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    79 Paraquat 10 mM 48 hrs, 4-day adult    97 Rotenone 2 μg 12 hrs, larvae L3    48 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    27 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    43 Cadmium 50 mM 12 hrs, larvae L3    74 Cadmium 50 mM 48 hrs, 4-day adult    49 Cadmium 100 mM 48 hrs, 4-day adult    74 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    59 Ethanol 2.5% 3 hrs, larvae L3    72 Ethanol 5% 3 hrs, larvae L3    55 Ethanol 10% 3 hrs, larvae L3    49 Caffeine 1.5 mg/ml 4 hrs, larvae L3    47 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    65 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    79 Paraquat 10 mM 48 hrs, 4-day adult    97 Rotenone 2 μg 12 hrs, larvae L3    48 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  30 cold shock, 4-day adult  27 heat shock, 4-day adult  (47) Cadmium 50 mM 6 hrs, larvae L3  (43) Cadmium 50 mM 12 hrs, larvae L3  (74) Cadmium 50 mM 48 hrs, 4-day adult  (49) Cadmium 100 mM 48 hrs, 4-day adult  (74) Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult  (44) Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult  (59) Ethanol 2.5% 3 hrs, larvae L3  (72) Ethanol 5% 3 hrs, larvae L3  (55) Ethanol 10% 3 hrs, larvae L3  (49) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (47) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (65) Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult  (79) Paraquat 10 mM 48 hrs, 4-day adult  (97) Rotenone 2 μg 12 hrs, larvae L3  (48) Rotenone 8 μg 12 hrs, larvae L3  (40) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    27 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    43 Cadmium 50 mM 12 hrs, larvae L3    74 Cadmium 50 mM 48 hrs, 4-day adult    49 Cadmium 100 mM 48 hrs, 4-day adult    74 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    59 Ethanol 2.5% 3 hrs, larvae L3    72 Ethanol 5% 3 hrs, larvae L3    55 Ethanol 10% 3 hrs, larvae L3    49 Caffeine 1.5 mg/ml 4 hrs, larvae L3    47 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    65 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    79 Paraquat 10 mM 48 hrs, 4-day adult    97 Rotenone 2 μg 12 hrs, larvae L3    48 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    27 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    43 Cadmium 50 mM 12 hrs, larvae L3    74 Cadmium 50 mM 48 hrs, 4-day adult    49 Cadmium 100 mM 48 hrs, 4-day adult    74 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    59 Ethanol 2.5% 3 hrs, larvae L3    72 Ethanol 5% 3 hrs, larvae L3    55 Ethanol 10% 3 hrs, larvae L3    49 Caffeine 1.5 mg/ml 4 hrs, larvae L3    47 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    65 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    79 Paraquat 10 mM 48 hrs, 4-day adult    97 Rotenone 2 μg 12 hrs, larvae L3    48 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_21 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • RE26756 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0036666 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9712-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for TSG101 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | first instar larval stage | recessive TSG1012 lethal | pupal stage, with Scer\GAL4pnr-MD237 TSG101GD14295 lethal | pupal stage | somatic clone TSG1012 lethal | somatic clone TSG1012 partially lethal - majority die | embryonic stage | recessive TSG1012 partially lethal - majority die | somatic clone | wandering third instar larval stage TSG1012 Other Phenotypes Allele hyperplasia | adult stage | cell non-autonomous | somatic clone TSG1012 hyperplasia | cell non-autonomous | somatic clone | wandering third instar larval stage TSG1012 large body | larval stage | somatic clone TSG1012 large body | somatic clone | wandering third instar larval stage TSG1012 Phenotype manifest in Allele embryonic/larval tracheal system TSG101942 TSG1011139 eye | somatic clone TSG1012 TSG101942 TSG1011139 eye-antennal disc | cell non-autonomous | somatic clone TSG1012 eye disc | somatic clone TSG1012 eye photoreceptor cell | cell non-autonomous | somatic clone TSG1012 eye photoreceptor cell | somatic clone TSG1012 head | somatic clone TSG1012 ommatidium | cell non-autonomous | somatic clone TSG1012 oocyte TSG1012 rhabdomere & eye photoreceptor cell | somatic clone TSG1012 terminal tracheal cell TSG101942 TSG1011139 vesicle TSG1012 yolk granule TSG1012
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of TSG101 Class Mutagen Stocks Known lesion TSG101e00381 1 -- TSG101f00976 piggyBac transposase 1 -- TSG1011139 ethyl methanesulfonate 0 Yes TSG1012 ethyl methanesulfonate 0 Yes TSG101942 ethyl methanesulfonate 0 Yes TSG101P26 hypomorphic allele - genetic evidence P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of TSG101 Class Mutagen Stocks Known lesion TSG101GD14295 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes TSG101GLV21075 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes TSG101HMS01768 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes TSG101hs.PM in vitro construct - regulatory fusion 0 Yes TSG101MtnA.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes TSG101NIG.9712R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Exel9002 (Ryder, 2004.4.26, Moberg et al., 2005, Ghabrial et al., 2011) Df(3L)Exel9003 (Ryder, 2004.4.26) Df(3L)Exel9004 (Ryder, 2004.4.26, Moberg et al., 2005)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14295} NA P{NIG.9712R} NA P{TRiP.GLV21075} NA P{TRiP.HMS01768} NA heat-shock construct P{hs-TSG101.M} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}TSG101e00381 NA PBac{WH}TSG101f00976 NA insertion of mobile activating element P{EPgy2}TSG101P26 NA P{GSV1}EP-666EP-666 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Vps28 (Sevrioukov et al., 2005)
Biological Process
CV term Evidence References
maintenance of apical/basal cell polarity inferred from mutant phenotype (Moberg et al., 2005) negative regulation of growth inferred from mutant phenotype (Moberg et al., 2005) negative regulation of growth of symbiont in host inferred from mutant phenotype (Philips et al., 2008) negative regulation of imaginal disc growth inferred from mutant phenotype (Moberg et al., 2005) regulation of JAK-STAT cascade inferred from mutant phenotype (Moberg et al., 2005) terminal branching, open tracheal system inferred from mutant phenotype (Ghabrial et al., 2011) trachea morphogenesis inferred from mutant phenotype (Ghabrial et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cellular protein modification process inferred from electronic annotation with InterPro:IPR008883 (FlyBase Curators et al., 2004-) protein transport inferred from electronic annotation with InterPro:IPR008883 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
TSG101 - CG1105 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) TSG101 - CG1115 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) TSG101 - CG14556 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) TSG101 - CG15891 experimental knowledge based (Guruharsha et al., 2011) TSG101 - CG2183 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) TSG101 - CG7192 experimental knowledge based (Guruharsha et al., 2011) TSG101 - Vps28 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) TSG101 - mib2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) TSG101 - mod(r) anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) TSG101 - stv anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions TSG101 allele Gene References unspecified Stat92E (Moberg et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 65180 DroID - A comprehensive database of gene and protein interactions. • FBgn0036666 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39881
Orthologs
OrthoDB Orthologs (50) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6BCF6W Dipteran inclusive ortholog search EOG61JZMT Insect inclusive ortholog search EOG60CGCH Arthropod inclusive ortholog search EOG6612KH Metazoa inclusive ortholog search EOG6J1D2R
Orthologs in Drosophila Species (EOG6BCF6W)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\TSG101     Drosophila simulans   Dsim\GD14667 Y   Drosophila sechellia   Dsec\GM25660 Y   Drosophila erecta   Dere\GG13581 Y   Drosophila yakuba   Dyak\GE19877 Y   Drosophila ananassae   Dana\GF23934 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA28575 Y   Drosophila persimilis   Dper\GL20850 Y   Drosophila willistoni   Dwil\GK16611 Y   Drosophila virilis   Dvir\GJ12339 Y   Drosophila mojavensis   Dmoj\GI12442 Y   Drosophila grimshawi   Dgri\GH16594 Y
Orthologs in non-Drosophila Dipterans (EOG61JZMT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012515   Anopheles gambiae Malaria mosquito  Agam\AGAP005934   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ020305   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006913   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002586   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015378
Orthologs in non-Dipteran Insects (EOG60CGCH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\TSG101   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG017411   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17538   Atta cephalotes Leafcutter ant  Acep\ACEP15818   Camponotus floridanus Florida carpenter ant  Cflo\CFLO12783   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21937   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21936   Linepithema humile Argentine ant  Lhum\LH17679   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18313   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000238   Bombyx mori Silkmoth  Bmor\BGIBMGA004136   Pediculus humanus Human body louse  Phum\PHUM537110   Tribolium castaneum Red flour beetle  Tcas\TC002425
Orthologs in non-Insect Arthropods (EOG6612KH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_65881   Ixodes scapularis Deer tick  Isca\ISCW014292
Orthologs in non-Arthropod Metazoa (EOG6J1D2R)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\tsg-101   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020950gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011963gn   Danio rerio Zebrafish  Drer\ENSDARG00000011897   Danio rerio Zebrafish  Drer\uevld   Danio rerio Zebrafish  Drer\tsg101   Danio rerio Zebrafish  Drer\ENSDARG00000086195   Xenopus tropicalis Western clawed frog  Xtro\tsg101   Xenopus tropicalis Western clawed frog  Xtro\uevld   Gallus gallus Domestic chicken  Ggal\UEVLD   Gallus gallus Domestic chicken  Ggal\TSG101   Mus musculus House mouse  Mmus\Uevld   Mus musculus House mouse  Mmus\Tsg101   Rattus norvegicus Norway rat  Rnor\LOC688000   Rattus norvegicus Norway rat  Rnor\Tsg101   Homo sapiens Human  Hsap\UEVLD   Homo sapiens Human  Hsap\TSG101
Human Orthologs (2)
Gene OMIM HGNC Hsap\UEVLD     Hsap\TSG101
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD14667 Drosophila sechellia Dsec\GM25660 Drosophila erecta Dere\GG13581 Drosophila yakuba Dyak\GE19877 Drosophila ananassae Dana\GF23934 Drosophila pseudoobscura pseudoobscura Dpse\GA28575 Drosophila persimilis Dper\GL20850 Drosophila willistoni Dwil\GK16611 Drosophila virilis Dvir\GJ12339 Drosophila mojavensis Dmoj\GI12442 Drosophila grimshawi Dgri\GH16594
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	35710 y1 sc* v1; P{TRiP.GLV21075}attP2 38306 y1 sc* v1; P{TRiP.HMS01768}attP40
Harvard	- PBac{RB}TSG101e00381 - PBac{WH}TSG101f00976
VDRC	v23944 w1118; P{GD14295}v23944/CyO
Genomic Clones ( 1 )
	BACR48E21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 62 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE26756
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT18675 AT20456 AT23186 GH09529 GH18254 GH22492 LD32419 LD47333 LP12350 RE37147 RE54653 RE73608 RH06637 RH10957 RH25148 RH29534 RH30168 RH36765 RH42447 SD14244 SD19834
Other clones	28853 51231 74524 273588 522238 522944 bs04c03 CK01.31C.G6 CK01.34D.C9 CK01.71A.E6 EC32741 EK056451 EK122501 EK136958 EN01633 EN03064 EN17303 ESG019d.E21_B01 FGC024B05 RP003017170 RP003043966 RP003118195 RP003150982 RP003181810 RP003222042 RP003241341 RP003269884 RP003291977 RP003315949 RP003316751 RP003342167 RP003434258 RP003451982 RP003464422 RP003491965 RP003559693 RP003574668 RP003581029 RP003656499 RP003665438
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0036666 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39881
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: TSG101 CG9712 (Laurencon, 2000.10.20)
Source for database merge of	Source for merge of: TSG101 ept (Moberg, 2005.6.19)
Additional comments
Other Comments
	Identification: In an ey-FLP system, based on its non-cell autonomous overgrowth phenotype. TSG101 is as member of a novel class of 'non-autonomous growth suppressor' genes. (Moberg et al., 2005) TSG101 is involved in ubiquitin-dependent regulation of proteins. (Moberg et al., 2005) TSG101 mutant cells in the eye-antennal disc survive poorly. The eyes and heads of flies that contain TSG101 mutant cells are dramatically overgrown, yet are composed of mostly wild type cells. TSG101 mutant cells hyperactivate N signaling and overproduce the secreted growth factor os. TSG101 mutant cells provoke local tissue hyperplasia and ectopic S phase entry in surrounding wild type cells; the resulting overgrowth is dependent upon signaling through the os-responsive JAK/STAT pathway. (Moberg et al., 2005)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079396 RefSeq (Proteins) • NP_524120 DDBJ / EMBL / GenBank • AAF49406 AAG29564 AAM52739 AAY82230 AAY82231 AF315343 AI110074 AI944688 AY122227 BI229055 CZ483456 CZ483457 DQ017452 DQ017453 Entrez Gene - A searchable database of RefSeq genes. • 39881 UniProtKB/TrEMBL • Q3YNA6 Q8MQZ0 Q9VVA7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ubiquitin E2 variant, N-terminal (IPR008883) Ubiquitin-conjugating enzyme/RWD-like (IPR016135) Steadiness box (IPR017916) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE26756
Linkouts
	BioGRID - A database of protein and genetic interactions • 65180 DroID - A comprehensive database of gene and protein interactions. • FBgn0036666 DRSC - Results from RNAi screens. • FBgn0036666 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0036666 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9712-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0036666 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39881 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/tsg101erupted1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39881 modMine - Data generated by the modENCODE project. • FBgn0036666
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	burs (Ghabrial et al., 2011) CG9712 (Stuart et al., 2007, Molnar et al., 2006, Ni et al., 2011.7.19, Vidal et al., 2010, Ni et al., 2010.12.1) DmTSG101 (Laurencon, 2000.10.20) dTsg101 (Sevrioukov et al., 2005, Philips et al., 2008) dTSg101 (Sevrioukov et al., 2005) dVps23 (Akbar et al., 2007) ept (Moberg et al., 2005, Gilbert et al., 2007, Giebel and Wodarz, 2006, Irion and St Johnston, 2007, Irion and St Johnston, 2007, Moberg et al., 2005, Gilbert et al., 2009, Gilbert et al., 2009, Saj et al., 2010, Grusche et al., 2011) ESCRT-I (Vaccari et al., 2008) Tsg101 (Gilbert and Moberg, 2006, Spradling, 2006, Irion and St Johnston, 2007, Miura et al., 2008, Philips et al., 2008) TSG101 (Stuart et al., 2007, Molnar et al., 2006, Halbsgut et al., 2011, Gilbert et al., 2009, Morozova et al., 2006, Brown and Feder, 2005, Vaccari et al., 2008, Japanese National Institute of Genetics, 2012.5.21) tsg101 (Menut et al., 2007, Vaccari et al., 2008, Gilbert et al., 2009, Vaccari et al., 2009, Vidal et al., 2010) vps23 (Ahmad et al., 2009, Uytterhoeven et al., 2011)
Name Synonym	erupted (Moberg et al., 2005, Gilbert et al., 2007, Giebel and Wodarz, 2006, Irion and St Johnston, 2007, Moberg et al., 2005, Haberman et al., 2008, Halbsgut et al., 2011, Gilbert et al., 2009, Gilbert et al., 2009, Grusche et al., 2011) Erupted (Childress et al., 2006, Childress et al., 2007, Vaccari et al., 2009) tumor suppressor protein 101 (Laurencon, 2000.10.20) Tumor Susceptibility Gene-101 (Gilbert et al., 2009)
Secondary FlyBase IDs
	FBgn0082594
References ( 60 )
Generate a list of	All references relating to this gene ( 60 )
List References by type	Research paper  ( 27 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 6 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Conference report  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 7 )
	Fisher et al., 2012, BMC Genomics 13: 506 Advances in genome-wide RNAi cellular screens: a case study using the Drosophila JAK/STAT pathway. [FBrf0220361] Ghabrial et al., 2011, PLoS Genet. 7(7): e1002087 A systematic screen for tube morphogenesis and branching genes in the Drosophila tracheal system. [FBrf0214368] Grusche et al., 2011, Dev. Biol. 350(2): 255--266 The Salvador/Warts/Hippo pathway controls regenerative tissue growth in Drosophila melanogaster. [FBrf0212888] Halbsgut et al., 2011, Mol. Biol. Cell 22(22): 4373--4379 Apical-basal polarity in Drosophila neuroblasts is independent of vesicular trafficking. [FBrf0216808] Hori et al., 2011, J. Cell Biol. 195(6): 1005--1015 Synergy between the ESCRT-III complex and Deltex defines a ligand-independent Notch signal. [FBrf0216916] Robinson and Moberg, 2011, Cell Cycle 10(23): 4110--4118 Drosophila endocytic neoplastic tumor suppressor genes regulate Sav/Wts/Hpo signaling and the c-Jun N-terminal kinase pathway. [FBrf0217243] Uytterhoeven et al., 2011, Cell 145(1): 117--132 Loss of skywalker reveals synaptic endosomes as sorting stations for synaptic vesicle proteins. [FBrf0213384] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...