Gene Dmel\MED19

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General Information

Symbol

Dmel\MED19

Species

D. melanogaster

Name

Mediator complex subunit 19

Annotation symbol

CG5546

Feature type

protein_coding_gene

FlyBase ID

FBgn0036761

Gene Model Status

Current

Stock availability

6 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

75A4-75A4

Sequence location

3L:17,836,915..17,839,601 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Mediator complex subunit 19 is referred to in FlyBase by the symbol Dmel\MED19 (CG5546, FBgn0036761). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: RNA polymerase II transcription cofactor activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: regulation of transcription from RNA polymerase II promoter. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Mediator complex, subunit Med19, metazoa. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval Malpighian tubules, larval/adult salivary gland, larval trachea, adult ovary, adult testis, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1640637_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of MED19. Gene sequence location is 3L:17836915..17839601.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

75A4-75A4

Limits computationally determined from genome sequence between P{lacW}l(3)j11B2^j11B2 and P{PZ}W⁰⁵⁰¹⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\MED19 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

MED19-RA

FBtr0075160

NM_140759

1763

337

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

MED19-PA

FBpp0074926

35.4

337

10.64

NP_649016

AAF49295

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Mediator complex, subunit Med19, metazoa (IPR019403)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VVL6

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval Malpighian tubules, larval/adult salivary gland, larval trachea, adult ovary, adult testis, larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		228.175
Larval Midgut		81
Larval Hindgut		97.2
Larval Malpighian Tubules		105.7
Larval Fat Body		98.6
Larval Salivary Gland		141.8
Larval Trachea		112.45
Larval Carcass		173.275
Adult Head		70.9
Adult Eye		66.575
Adult Brain		164.2
Adult Thoracic-Abdominal Ganglion		144.1
Adult Crop		92.1
Adult Midgut		69.1
Adult Hindgut		79.4
Adult Malpighian Tubules		94.2
Adult Fat Body		60.3
Adult Salivary Gland		124.2
Adult Heart		57.325
Adult VirginFemale Spermatheca		63
Adult InseminatedFemale Spermatheca		56.9
Adult Ovary		373.7
Adult Testis		160.9
Adult Male Accessory Gland		79.5
Adult Carcass		73.3
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		228.175
Larval Midgut		81
Larval Hindgut		97.2
Larval Malpighian Tubules		105.7
Larval Fat Body		98.6
Larval Salivary Gland		141.8
Larval Trachea		112.45
Larval Carcass		173.275
Adult Head		70.9
Adult Eye		66.575
Adult Brain		164.2
Adult Thoracic-Abdominal Ganglion		144.1
Adult Crop		92.1
Adult Midgut		69.1
Adult Hindgut		79.4
Adult Malpighian Tubules		94.2
Adult Fat Body		60.3
Adult Salivary Gland		124.2
Adult Heart		57.325
Adult VirginFemale Spermatheca		63
Adult InseminatedFemale Spermatheca		56.9
Adult Ovary		373.7
Adult Testis		160.9
Adult Male Accessory Gland		79.5
Adult Carcass		73.3
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		228.175
Larval Midgut		81
Larval Hindgut		97.2
Larval Malpighian Tubules		105.7
Larval Fat Body		98.6
Larval Salivary Gland		141.8
Larval Trachea		112.45
Larval Carcass		173.275
Adult Head		70.9
Adult Eye		66.575
Adult Brain		164.2
Adult Thoracic-Abdominal Ganglion		144.1
Adult Crop		92.1
Adult Midgut		69.1
Adult Hindgut		79.4
Adult Malpighian Tubules		94.2
Adult Fat Body		60.3
Adult Salivary Gland		124.2
Adult Heart		57.325
Adult VirginFemale Spermatheca		63
Adult InseminatedFemale Spermatheca		56.9
Adult Ovary		373.7
Adult Testis		160.9
Adult Male Accessory Gland		79.5
Adult Carcass		73.3
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		228.175
Larval Midgut		81
Larval Hindgut		97.2
Larval Malpighian Tubules		105.7
Larval Fat Body		98.6
Larval Salivary Gland		141.8
Larval Trachea		112.45
Larval Carcass		173.275
Adult Head		70.9
Adult Eye		66.575
Adult Brain		164.2
Adult Thoracic-Abdominal Ganglion		144.1
Adult Crop		92.1
Adult Midgut		69.1
Adult Hindgut		79.4
Adult Malpighian Tubules		94.2
Adult Fat Body		60.3
Adult Salivary Gland		124.2
Adult Heart		57.325
Adult VirginFemale Spermatheca		63
Adult InseminatedFemale Spermatheca		56.9
Adult Ovary		373.7
Adult Testis		160.9
Adult Male Accessory Gland		79.5
Adult Carcass		73.3
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		228.175
Larval Midgut		81
Larval Hindgut		97.2
Larval Malpighian Tubules		105.7
Larval Fat Body		98.6
Larval Salivary Gland		141.8
Larval Trachea		112.45
Larval Carcass		173.275
Adult Head		70.9
Adult Eye		66.575
Adult Brain		164.2
Adult Thoracic-Abdominal Ganglion		144.1
Adult Crop		92.1
Adult Midgut		69.1
Adult Hindgut		79.4
Adult Malpighian Tubules		94.2
Adult Fat Body		60.3
Adult Salivary Gland		124.2
Adult Heart		57.325
Adult VirginFemale Spermatheca		63
Adult InseminatedFemale Spermatheca		56.9
Adult Ovary		373.7
Adult Testis		160.9
Adult Male Accessory Gland		79.5
Adult Carcass		73.3
Expression Level Scale		None Low Moderate High

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		228.175
Larval Midgut		81
Larval Hindgut		97.2
Larval Malpighian Tubules		105.7
Larval Fat Body		98.6
Larval Salivary Gland		141.8
Larval Trachea		112.45
Larval Carcass		173.275
Adult Head		70.9
Adult Eye		66.575
Adult Brain		164.2
Adult Thoracic-Abdominal Ganglion		144.1
Adult Crop		92.1
Adult Midgut		69.1
Adult Hindgut		79.4
Adult Malpighian Tubules		94.2
Adult Fat Body		60.3
Adult Salivary Gland		124.2
Adult Heart		57.325
Adult VirginFemale Spermatheca		63
Adult InseminatedFemale Spermatheca		56.9
Adult Ovary		373.7
Adult Testis		160.9
Adult Male Accessory Gland		79.5
Adult Carcass		73.3
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		228.175
Larval Midgut		81
Larval Hindgut		97.2
Larval Malpighian Tubules		105.7
Larval Fat Body		98.6
Larval Salivary Gland		141.8
Larval Trachea		112.45
Larval Carcass		173.275
Adult Head		70.9
Adult Eye		66.575
Adult Brain		164.2
Adult Thoracic-Abdominal Ganglion		144.1
Adult Crop		92.1
Adult Midgut		69.1
Adult Hindgut		79.4
Adult Malpighian Tubules		94.2
Adult Fat Body		60.3
Adult Salivary Gland		124.2
Adult Heart		57.325
Adult VirginFemale Spermatheca		63
Adult InseminatedFemale Spermatheca		56.9
Adult Ovary		373.7
Adult Testis		160.9
Adult Male Accessory Gland		79.5
Adult Carcass		73.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		228.175
Larval Midgut		81
Larval Hindgut		97.2
Larval Malpighian Tubules		105.7
Larval Fat Body		98.6
Larval Salivary Gland		141.8
Larval Trachea		112.45
Larval Carcass		173.275
Adult Head		70.9
Adult Eye		66.575
Adult Brain		164.2
Adult Thoracic-Abdominal Ganglion		144.1
Adult Crop		92.1
Adult Midgut		69.1
Adult Hindgut		79.4
Adult Malpighian Tubules		94.2
Adult Fat Body		60.3
Adult Salivary Gland		124.2
Adult Heart		57.325
Adult VirginFemale Spermatheca		63
Adult InseminatedFemale Spermatheca		56.9
Adult Ovary		373.7
Adult Testis		160.9
Adult Male Accessory Gland		79.5
Adult Carcass		73.3
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	70.9
NA	Eye	66.575
NA	Brain	164.2
228.175	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	144.1
NA	Crop	92.1
81	Midgut	69.1
97.2	Hindgut	79.4
105.7	Malpighian Tubules	94.2
98.6	Fat Body	60.3
141.8	Salivary Gland	124.2
NA	Heart	57.325
112.45	Trachea	NA
NA	VirginFemale Spermatheca	63
NA	InseminatedFemale Spermatheca	56.9
NA	Ovary	373.7
NA	Testis	160.9
NA	Male Accessory Gland	79.5
173.275	Carcass	73.3

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		55
central nervous system, pupae P8		21
head, virgin 1-day female		10
head, virgin 4-day female		11
head, virgin 20-day female		8
head, mated 1-day female		12
head, mated 4-day female		11
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		19
head, mated 20-day male		22
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		28
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		9
digestive system, 4-day adult		16
digestive system, 20-day adult		11
fat body, larvae L3 wandering		10
fat body, white prepupae		15
fat body, pupae P8		23
carcass, larvae L3 wandering		69
carcass, 1-day adult		10
carcass, 4-day adult		13
carcass, 20-day adult		12
ovary, virgin 4-day female		86
ovary, mated 4-day female		89
testis, mated 4-day male		39
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(60)
central nervous system, larvae L3		(55)
central nervous system, pupae P8		21
head, virgin 1-day female		10
head, virgin 4-day female		11
head, virgin 20-day female		8
head, mated 1-day female		12
head, mated 4-day female		11
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		19
head, mated 20-day male		22
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		(28)
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		9
digestive system, 4-day adult		16
digestive system, 20-day adult		11
fat body, larvae L3 wandering		10
fat body, white prepupae		15
fat body, pupae P8		23
carcass, larvae L3 wandering		(69)
carcass, 1-day adult		10
carcass, 4-day adult		13
carcass, 20-day adult		12
ovary, virgin 4-day female		(86)
ovary, mated 4-day female		(89)
testis, mated 4-day male		(39)
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		55
central nervous system, pupae P8		21
head, virgin 1-day female		10
head, virgin 4-day female		11
head, virgin 20-day female		8
head, mated 1-day female		12
head, mated 4-day female		11
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		19
head, mated 20-day male		22
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		28
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		9
digestive system, 4-day adult		16
digestive system, 20-day adult		11
fat body, larvae L3 wandering		10
fat body, white prepupae		15
fat body, pupae P8		23
carcass, larvae L3 wandering		69
carcass, 1-day adult		10
carcass, 4-day adult		13
carcass, 20-day adult		12
ovary, virgin 4-day female		86
ovary, mated 4-day female		89
testis, mated 4-day male		39
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		55
central nervous system, pupae P8		21
head, virgin 1-day female		10
head, virgin 4-day female		11
head, virgin 20-day female		8
head, mated 1-day female		12
head, mated 4-day female		11
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		19
head, mated 20-day male		22
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		28
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		9
digestive system, 4-day adult		16
digestive system, 20-day adult		11
fat body, larvae L3 wandering		10
fat body, white prepupae		15
fat body, pupae P8		23
carcass, larvae L3 wandering		69
carcass, 1-day adult		10
carcass, 4-day adult		13
carcass, 20-day adult		12
ovary, virgin 4-day female		86
ovary, mated 4-day female		89
testis, mated 4-day male		39
accessory gland, mated 4-day male		8
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		55
central nervous system, pupae P8		21
head, virgin 1-day female		10
head, virgin 4-day female		11
head, virgin 20-day female		8
head, mated 1-day female		12
head, mated 4-day female		11
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		19
head, mated 20-day male		22
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		28
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		9
digestive system, 4-day adult		16
digestive system, 20-day adult		11
fat body, larvae L3 wandering		10
fat body, white prepupae		15
fat body, pupae P8		23
carcass, larvae L3 wandering		69
carcass, 1-day adult		10
carcass, 4-day adult		13
carcass, 20-day adult		12
ovary, virgin 4-day female		86
ovary, mated 4-day female		89
testis, mated 4-day male		39
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(60)
central nervous system, larvae L3		(55)
central nervous system, pupae P8		21
head, virgin 1-day female		10
head, virgin 4-day female		11
head, virgin 20-day female		8
head, mated 1-day female		12
head, mated 4-day female		11
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		19
head, mated 20-day male		22
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		28
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		9
digestive system, 4-day adult		16
digestive system, 20-day adult		11
fat body, larvae L3 wandering		10
fat body, white prepupae		15
fat body, pupae P8		23
carcass, larvae L3 wandering		(69)
carcass, 1-day adult		10
carcass, 4-day adult		13
carcass, 20-day adult		12
ovary, virgin 4-day female		(86)
ovary, mated 4-day female		(89)
testis, mated 4-day male		(39)
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		55
central nervous system, pupae P8		21
head, virgin 1-day female		10
head, virgin 4-day female		11
head, virgin 20-day female		8
head, mated 1-day female		12
head, mated 4-day female		11
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		19
head, mated 20-day male		22
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		28
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		9
digestive system, 4-day adult		16
digestive system, 20-day adult		11
fat body, larvae L3 wandering		10
fat body, white prepupae		15
fat body, pupae P8		23
carcass, larvae L3 wandering		69
carcass, 1-day adult		10
carcass, 4-day adult		13
carcass, 20-day adult		12
ovary, virgin 4-day female		86
ovary, mated 4-day female		89
testis, mated 4-day male		39
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		55
central nervous system, pupae P8		21
head, virgin 1-day female		10
head, virgin 4-day female		11
head, virgin 20-day female		8
head, mated 1-day female		12
head, mated 4-day female		11
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		19
head, mated 20-day male		22
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		28
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		9
digestive system, 4-day adult		16
digestive system, 20-day adult		11
fat body, larvae L3 wandering		10
fat body, white prepupae		15
fat body, pupae P8		23
carcass, larvae L3 wandering		69
carcass, 1-day adult		10
carcass, 4-day adult		13
carcass, 20-day adult		12
ovary, virgin 4-day female		86
ovary, mated 4-day female		89
testis, mated 4-day male		39
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, during early pupal stages, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		89
embryo 02-04hr		39
embryo 04-06hr		68
embryo 06-08hr		73
embryo 08-10hr		59
embryo 10-12hr		58
embryo 12-14hr		34
embryo 14-16hr		24
embryo 16-18hr		21
embryo 18-20hr		21
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		16
larva L2		12
larva L3 12hr old		9
larva L3 puffstage 1-2		14
larva L3 puffstage 3-6		26
larva L3 puffstage 7-9		59
white prepupae new		76
white prepupae 12hr		34
white prepupae 24hr		39
pupae 2d postWPP		26
pupae 3d postWPP		15
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		17
adult male 30day		16
adult female 01day		27
adult female 05day		54
adult female 30day		56
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(89)
embryo 02-04hr		(39)
embryo 04-06hr		(68)
embryo 06-08hr		(73)
embryo 08-10hr		(59)
embryo 10-12hr		(58)
embryo 12-14hr		(34)
embryo 14-16hr		24
embryo 16-18hr		21
embryo 18-20hr		21
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		16
larva L2		12
larva L3 12hr old		9
larva L3 puffstage 1-2		14
larva L3 puffstage 3-6		26
larva L3 puffstage 7-9		(59)
white prepupae new		(76)
white prepupae 12hr		(34)
white prepupae 24hr		(39)
pupae 2d postWPP		26
pupae 3d postWPP		15
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		17
adult male 30day		16
adult female 01day		(27)
adult female 05day		(54)
adult female 30day		(56)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		89
embryo 02-04hr		39
embryo 04-06hr		68
embryo 06-08hr		73
embryo 08-10hr		59
embryo 10-12hr		58
embryo 12-14hr		34
embryo 14-16hr		24
embryo 16-18hr		21
embryo 18-20hr		21
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		16
larva L2		12
larva L3 12hr old		9
larva L3 puffstage 1-2		14
larva L3 puffstage 3-6		26
larva L3 puffstage 7-9		59
white prepupae new		76
white prepupae 12hr		34
white prepupae 24hr		39
pupae 2d postWPP		26
pupae 3d postWPP		15
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		17
adult male 30day		16
adult female 01day		27
adult female 05day		54
adult female 30day		56
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		89
embryo 02-04hr		39
embryo 04-06hr		68
embryo 06-08hr		73
embryo 08-10hr		59
embryo 10-12hr		58
embryo 12-14hr		34
embryo 14-16hr		24
embryo 16-18hr		21
embryo 18-20hr		21
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		16
larva L2		12
larva L3 12hr old		9
larva L3 puffstage 1-2		14
larva L3 puffstage 3-6		26
larva L3 puffstage 7-9		59
white prepupae new		76
white prepupae 12hr		34
white prepupae 24hr		39
pupae 2d postWPP		26
pupae 3d postWPP		15
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		17
adult male 30day		16
adult female 01day		27
adult female 05day		54
adult female 30day		56
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		89
embryo 02-04hr		39
embryo 04-06hr		68
embryo 06-08hr		73
embryo 08-10hr		59
embryo 10-12hr		58
embryo 12-14hr		34
embryo 14-16hr		24
embryo 16-18hr		21
embryo 18-20hr		21
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		16
larva L2		12
larva L3 12hr old		9
larva L3 puffstage 1-2		14
larva L3 puffstage 3-6		26
larva L3 puffstage 7-9		59
white prepupae new		76
white prepupae 12hr		34
white prepupae 24hr		39
pupae 2d postWPP		26
pupae 3d postWPP		15
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		17
adult male 30day		16
adult female 01day		27
adult female 05day		54
adult female 30day		56
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(89)
embryo 02-04hr		(39)
embryo 04-06hr		(68)
embryo 06-08hr		(73)
embryo 08-10hr		(59)
embryo 10-12hr		(58)
embryo 12-14hr		(34)
embryo 14-16hr		24
embryo 16-18hr		21
embryo 18-20hr		21
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		16
larva L2		12
larva L3 12hr old		9
larva L3 puffstage 1-2		14
larva L3 puffstage 3-6		26
larva L3 puffstage 7-9		(59)
white prepupae new		(76)
white prepupae 12hr		(34)
white prepupae 24hr		(39)
pupae 2d postWPP		26
pupae 3d postWPP		15
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		17
adult male 30day		16
adult female 01day		27
adult female 05day		(54)
adult female 30day		(56)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		89
embryo 02-04hr		39
embryo 04-06hr		68
embryo 06-08hr		73
embryo 08-10hr		59
embryo 10-12hr		58
embryo 12-14hr		34
embryo 14-16hr		24
embryo 16-18hr		21
embryo 18-20hr		21
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		16
larva L2		12
larva L3 12hr old		9
larva L3 puffstage 1-2		14
larva L3 puffstage 3-6		26
larva L3 puffstage 7-9		59
white prepupae new		76
white prepupae 12hr		34
white prepupae 24hr		39
pupae 2d postWPP		26
pupae 3d postWPP		15
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		17
adult male 30day		16
adult female 01day		27
adult female 05day		54
adult female 30day		56
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		89
embryo 02-04hr		39
embryo 04-06hr		68
embryo 06-08hr		73
embryo 08-10hr		59
embryo 10-12hr		58
embryo 12-14hr		34
embryo 14-16hr		24
embryo 16-18hr		21
embryo 18-20hr		21
embryo 20-22hr		15
embryo 22-24hr		16
larva L1		16
larva L2		12
larva L3 12hr old		9
larva L3 puffstage 1-2		14
larva L3 puffstage 3-6		26
larva L3 puffstage 7-9		59
white prepupae new		76
white prepupae 12hr		34
white prepupae 24hr		39
pupae 2d postWPP		26
pupae 3d postWPP		15
pupae 4d postWPP		18
adult male 01day		15
adult male 05day		17
adult male 30day		16
adult female 01day		27
adult female 05day		54
adult female 30day		56
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		49
embryonic 1182-4H		40
embryonic GM2		29
embryonic Kc167		36
embryonic S1		43
embryonic S3		23
leg disc CME L1		54
wing disc CME-W2		17
wing disc ML-DmD8		26
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		36
wing disc ML-DmD32		30
haltere disc ML-DmD17-c3		31
eye-antennal disc ML-DmD11		34
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		44
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		28
ovary fGS/OSS		38
ovary OSC		36
ovary OSS		24
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(42)
Schneider line 2 Sg4		(49)
embryonic 1182-4H		(40)
embryonic GM2		(29)
embryonic Kc167		(36)
embryonic S1		(43)
embryonic S3		23
leg disc CME L1		(54)
wing disc CME-W2		17
wing disc ML-DmD8		26
wing disc ML-DmD9		(37)
wing disc ML-DmD16-c3		(58)
wing disc ML-DmD21		(36)
wing disc ML-DmD32		(30)
haltere disc ML-DmD17-c3		(31)
eye-antennal disc ML-DmD11		(34)
antennal disc ML-DmD20-c5		(28)
mixed discs ML-DmD4-c1		(30)
CNS ML-DmBG1-c1		(44)
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		(28)
ovary fGS/OSS		(38)
ovary OSC		(36)
ovary OSS		24
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		49
embryonic 1182-4H		40
embryonic GM2		29
embryonic Kc167		36
embryonic S1		43
embryonic S3		23
leg disc CME L1		54
wing disc CME-W2		17
wing disc ML-DmD8		26
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		36
wing disc ML-DmD32		30
haltere disc ML-DmD17-c3		31
eye-antennal disc ML-DmD11		34
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		44
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		28
ovary fGS/OSS		38
ovary OSC		36
ovary OSS		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		49
embryonic 1182-4H		40
embryonic GM2		29
embryonic Kc167		36
embryonic S1		43
embryonic S3		23
leg disc CME L1		54
wing disc CME-W2		17
wing disc ML-DmD8		26
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		36
wing disc ML-DmD32		30
haltere disc ML-DmD17-c3		31
eye-antennal disc ML-DmD11		34
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		44
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		28
ovary fGS/OSS		38
ovary OSC		36
ovary OSS		24
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		49
embryonic 1182-4H		40
embryonic GM2		29
embryonic Kc167		36
embryonic S1		43
embryonic S3		23
leg disc CME L1		54
wing disc CME-W2		17
wing disc ML-DmD8		26
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		36
wing disc ML-DmD32		30
haltere disc ML-DmD17-c3		31
eye-antennal disc ML-DmD11		34
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		44
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		28
ovary fGS/OSS		38
ovary OSC		36
ovary OSS		24
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(42)
Schneider line 2 Sg4		(49)
embryonic 1182-4H		(40)
embryonic GM2		29
embryonic Kc167		(36)
embryonic S1		(43)
embryonic S3		23
leg disc CME L1		(54)
wing disc CME-W2		17
wing disc ML-DmD8		26
wing disc ML-DmD9		(37)
wing disc ML-DmD16-c3		(58)
wing disc ML-DmD21		(36)
wing disc ML-DmD32		30
haltere disc ML-DmD17-c3		31
eye-antennal disc ML-DmD11		(34)
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		(44)
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		28
ovary fGS/OSS		(38)
ovary OSC		(36)
ovary OSS		24
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		49
embryonic 1182-4H		40
embryonic GM2		29
embryonic Kc167		36
embryonic S1		43
embryonic S3		23
leg disc CME L1		54
wing disc CME-W2		17
wing disc ML-DmD8		26
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		36
wing disc ML-DmD32		30
haltere disc ML-DmD17-c3		31
eye-antennal disc ML-DmD11		34
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		44
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		28
ovary fGS/OSS		38
ovary OSC		36
ovary OSS		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		49
embryonic 1182-4H		40
embryonic GM2		29
embryonic Kc167		36
embryonic S1		43
embryonic S3		23
leg disc CME L1		54
wing disc CME-W2		17
wing disc ML-DmD8		26
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		36
wing disc ML-DmD32		30
haltere disc ML-DmD17-c3		31
eye-antennal disc ML-DmD11		34
antennal disc ML-DmD20-c5		28
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		44
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		28
ovary fGS/OSS		38
ovary OSC		36
ovary OSS		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		22
heat shock, 4-day adult		31
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		69
Copper 0.5 mM 12 hrs, larvae L3		14
Copper 15 mM 48 hrs, 4-day adult		43
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		53
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		65
Caffeine 25 mg/ml 48 hrs, 4-day adult		39
Paraquat 5 mM 48 hrs, 4-day adult		50
Paraquat 10 mM 48 hrs, 4-day adult		34
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(33)
cold shock, 4-day adult		22
heat shock, 4-day adult		(31)
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		(27)
Cadmium 100 mM 48 hrs, 4-day adult		(69)
Copper 0.5 mM 12 hrs, larvae L3		14
Copper 15 mM 48 hrs, 4-day adult		(43)
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		(53)
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(65)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(39)
Paraquat 5 mM 48 hrs, 4-day adult		(50)
Paraquat 10 mM 48 hrs, 4-day adult		(34)
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		22
heat shock, 4-day adult		31
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		69
Copper 0.5 mM 12 hrs, larvae L3		14
Copper 15 mM 48 hrs, 4-day adult		43
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		53
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		65
Caffeine 25 mg/ml 48 hrs, 4-day adult		39
Paraquat 5 mM 48 hrs, 4-day adult		50
Paraquat 10 mM 48 hrs, 4-day adult		34
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		22
heat shock, 4-day adult		31
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		69
Copper 0.5 mM 12 hrs, larvae L3		14
Copper 15 mM 48 hrs, 4-day adult		43
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		53
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		65
Caffeine 25 mg/ml 48 hrs, 4-day adult		39
Paraquat 5 mM 48 hrs, 4-day adult		50
Paraquat 10 mM 48 hrs, 4-day adult		34
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		22
heat shock, 4-day adult		31
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		69
Copper 0.5 mM 12 hrs, larvae L3		14
Copper 15 mM 48 hrs, 4-day adult		43
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		53
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		65
Caffeine 25 mg/ml 48 hrs, 4-day adult		39
Paraquat 5 mM 48 hrs, 4-day adult		50
Paraquat 10 mM 48 hrs, 4-day adult		34
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(33)
cold shock, 4-day adult		22
heat shock, 4-day adult		31
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		(69)
Copper 0.5 mM 12 hrs, larvae L3		14
Copper 15 mM 48 hrs, 4-day adult		(43)
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		(53)
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(65)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(39)
Paraquat 5 mM 48 hrs, 4-day adult		(50)
Paraquat 10 mM 48 hrs, 4-day adult		(34)
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		22
heat shock, 4-day adult		31
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		69
Copper 0.5 mM 12 hrs, larvae L3		14
Copper 15 mM 48 hrs, 4-day adult		43
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		53
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		65
Caffeine 25 mg/ml 48 hrs, 4-day adult		39
Paraquat 5 mM 48 hrs, 4-day adult		50
Paraquat 10 mM 48 hrs, 4-day adult		34
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		22
heat shock, 4-day adult		31
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		69
Copper 0.5 mM 12 hrs, larvae L3		14
Copper 15 mM 48 hrs, 4-day adult		43
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		53
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		65
Caffeine 25 mg/ml 48 hrs, 4-day adult		39
Paraquat 5 mM 48 hrs, 4-day adult		50
Paraquat 10 mM 48 hrs, 4-day adult		34
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0036761

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG5546-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | pupal stage, with Scer\GAL4^pnr-MD237

MED19^GD11688

viable

MED19^f07123

Sterility

Allele

fertile

MED19^f07123

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of MED19	Class	Mutagen	Stocks	Known lesion
MED19^f07123		piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Allele of MED19	Mutagen	Stocks	Known lesion
MED19^GD11688	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
MED19^HMS00588	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
MED19^JF02713	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
MED19^KK102825	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
MED19^dsRNA.cMa	in vitro construct - RNAi	0	--
MED19^NIG.5546R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}MED19^f07123

Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 3 terms )

Molecular Function

CV term

Evidence

References

RNA polymerase II transcription cofactor activity

inferred from electronic annotation with InterPro:IPR019403

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

regulation of transcription from RNA polymerase II promoter

inferred from electronic annotation with InterPro:IPR019403

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

mediator complex

inferred from electronic annotation with InterPro:IPR019403

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Bourbon et al., 2004)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

MED19 - CG10681

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

MED19 - CG12493

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

MED19 - fan

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

MED19 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

65272

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0036761

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

39987

Orthologs

OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6HMJWT

Dipteran inclusive ortholog search

EOG6ZW5MC

Insect inclusive ortholog search

EOG698SX8

Arthropod inclusive ortholog search

EOG65DV5V

Metazoa inclusive ortholog search

EOG6SR8RK

Orthologs in Drosophila Species (EOG6HMJWT)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18960

Drosophila persimilis

Dper\GL22742

Drosophila willistoni

Dwil\GK21178

Drosophila virilis

Dvir\GJ13943

Drosophila mojavensis

Dmoj\GI13607

Drosophila grimshawi

Dgri\GH14462

Orthologs in non-Drosophila Dipterans (EOG6ZW5MC)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\MED19

Anopheles gambiae

Malaria mosquito

Agam\AGAP011626

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ013981

Orthologs in non-Dipteran Insects (EOG698SX8)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB44990

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012236

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH15922

Atta cephalotes

Leafcutter ant

Acep\ACEP14621

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO20973

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL19978

Linepithema humile

Argentine ant

Lhum\LH17613

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB20225

Solenopsis invicta

Red fire ant

Sinv\SINV13043

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003764

Bombyx mori

Silkmoth

Bmor\BGIBMGA002887

Pediculus humanus

Human body louse

Phum\PHUM250710

Tribolium castaneum

Red flour beetle

Tcas\TC011979

Orthologs in non-Insect Arthropods (EOG65DV5V)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\TFDP2

Ixodes scapularis

Deer tick

Isca\ISCW016111

Orthologs in non-Arthropod Metazoa (EOG6SR8RK)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\mdt-19

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_027874gn

Danio rerio

Zebrafish

Drer\med19a

Danio rerio

Zebrafish

Drer\med19

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000032150

Gallus gallus

Domestic chicken

Ggal\MED19

Mus musculus

House mouse

Mmus\Med19

Rattus norvegicus

Norway rat

Rnor\Med19

Homo sapiens

Human

Hsap\ENSG00000156603

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\ENSG00000156603

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18960

Drosophila persimilis

Dper\GL22742

Drosophila willistoni

Dwil\GK21178

Drosophila virilis

Dvir\GJ13943

Drosophila mojavensis

Dmoj\GI13607

Drosophila grimshawi

Dgri\GH14462

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

Bloomington

19047

w¹¹¹⁸; PBac{WH}MED19^f07123

27559

y¹ v¹; P{TRiP.JF02713}attP2

33710

y¹ sc^* v¹; P{TRiP.HMS00588}attP2

v103926

v27457

w¹¹¹⁸; P{GD11688}v27457/TM3

Genomic Clones ( 1 )

BACR17C17

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 81 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD41395

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0036761

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

39987

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: MED19 CG5546

(Bourbon et al., 2004)

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_140759

RefSeq (Proteins)

•

NP_649016

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

39987

UniProtKB/Swiss-Prot

•

Q9VVL6

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Mediator complex, subunit Med19, metazoa (IPR019403)

Linkouts

BioGRID - A database of protein and genetic interactions

•

65272

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0036761

DRSC - Results from RNAi screens.

•

FBgn0036761

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0036761

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG5546-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0036761

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

39987

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

39987

modMine - Data generated by the modENCODE project.

•

FBgn0036761

Synonyms & Secondary IDs ( 3 )

Reported As

Symbol Synonym

CG5546

(Bourbon et al., 2004, Guglielmi et al., 2004, Baeg et al., 2007, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2010.12.1)

MED19

(Ma et al., 2006, Tokusumi et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Felix et al., 2012)

Name Synonym

Mediator complex subunit 19

(Bourbon et al., 2004)

Secondary FlyBase IDs

References ( 22 )

Generate a list of

All references relating to this gene ( 22 )

List References by type

Research paper ( 4 )
Supplementary material ( 4 )
Erratum ( 1 )
Personal communication to FlyBase ( 10 )
Letter ( 1 )
Stock list ( 1 )

Recent research papers ( 2 )

Felix et al., 2012, Genetics 191(3): 989--1002: Age-Specific Variation in Immune Response in Drosophila melanogaster Has a Genetic Basis. [FBrf0218944]
Tokusumi et al., 2012, PLoS ONE 7(7): e41604: Gene regulatory networks controlling hematopoietic progenitor niche cell production and differentiation in the Drosophila lymph gland. [FBrf0219204]

Show all research papers ...