FlyBase Gene Report: Dmel\Tsp74F

General Information

Symbol

Dmel\Tsp74F

Species

D. melanogaster

Name

Tetraspanin 74F

Annotation Symbol

CG5492

Feature Type

protein_coding_gene

FlyBase ID

FBgn0036769

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Tetraspanin 74F (Tsp74F) encodes encodes a tetraspanin, which spans the membrane four times. When the products of both Tsp74F and Tsp66E are removed, this results in defects in actin polarization, endocytosis, and distribution of the integrin subunit encoded by if during oogenesis. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

75A8-75A8

Sequence location

3L:17,891,820..17,895,862 [-]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:17891820..17895862

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (1 term)

Molecular Function (0 terms)

There is no information supporting a Molecular Function term for this gene. (Based on a review of available data on: 2011-06-30 )

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in integral component of membrane

inferred from sequence model

(Fradkin et al., 2002)

inferred from electronic annotation with InterPro:IPR000301, InterPro:IPR008952, InterPro:IPR018499

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Gene Group (FlyBase)

TETRASPANINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

TETRASPANINS -: Tetraspanins (TSP) have four transmembrane domains and two extracellular loops containing specific short motifs, including highly conserved cysteine residues. TSPs are thought to function as scaffolds that bring multiple proteins - including other TSPs, membrane-associated proteins, extracellular and cytosolic proteins - into single membrane region. These regions are called tetraspanin-enriched microdomains. (Adapted from FBrf0227776 and FBrf0152316).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Tsp74F for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.46

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Tsp74F-RA

FBtr0075154

NM_168754

1414

236

Tsp74F-RB

FBtr0075155

NM_079408

1519

236

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Tsp74F-PA

FBpp0074920

26.5

236

7.04

NP_730317

AAN11686

Tsp74F-PB

FBpp0074921

26.5

236

7.04

NP_524132

AAF49286

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

236 aa isoforms: Tsp74F-PA, Tsp74F-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q7JZI2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tsp74F using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Tsp74F_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

wandering third instar larval stage

wing disc

(Han et al., 2012)

adult stage | female & oogenesis

egg chamber

(Han et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Tsp74F in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Tsp74F

View exonic expression by tissue for Dmel\Tsp74F

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036769

DRscDB

39995/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0036769

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5492-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5492

Flygut - An atlas of the Drosophila adult midgut

FBgn0036769

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Tsp74F

Stages(s) 1-3
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Tsp74F

Transgenic constructs containing regulatory region of Tsp74F

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | maternal effect

Tsp74F¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Tsp74F^GD4619

viable, with Scer\GAL4^Mef2.PR

Tsp74F^GD4619

viable, with Scer\GAL4^pnr-MD237

Tsp74F^GD4619

viable, with Scer\GAL4^tin.CΔ4

Tsp74F^GD4619

Other Phenotypes

Allele

some die during embryonic stage | maternal effect

Tsp74F¹

Phenotype manifest in

Allele

adult pericardial cell, with Scer\GAL4^Ugt36A1.PK

Tsp74F^HMS01966

egg | maternal effect

Tsp74F¹

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CD151

7 of 15

Yes

Hsap\TSPAN11

6 of 15

Yes

Hsap\CD63

1 of 15

Hsap\TSPAN1

1 of 15

Yes

Hsap\TSPAN3

1 of 15

Yes

Hsap\TSPAN12

1 of 15

Hsap\TSPAN16

1 of 15

Yes

Hsap\TSPAN18

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Cd151

7 of 15

Yes

Mmus\Tspan11

5 of 15

Yes

Mmus\Cd63

1 of 15

Mmus\Tspan1

1 of 15

Yes

Mmus\Tspan3

1 of 15

Yes

Mmus\Tspan12

1 of 15

Mmus\Tspan18

1 of 15

Rattus norvegicus (Norway rat) (7)

Rnor\LOC100911730

5 of 13

Yes

Rnor\Tspan11

5 of 13

Yes

Rnor\Cd151

3 of 13

Yes

Rnor\Tspan1

1 of 13

Yes

Rnor\Tspan3

1 of 13

Yes

Rnor\Tspan12

1 of 13

Yes

Rnor\Tspan18

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (8)

Xtro\cd151

6 of 12

Yes

Xtro\LOC101732684

2 of 12

Yes

Xtro\cd81

1 of 12

Xtro\LOC100487852

1 of 12

Yes

Xtro\LOC101734690

1 of 12

Xtro\tspan1

1 of 12

Yes

Xtro\tspan16

1 of 12

Yes

Xtro\tspan18

1 of 12

Danio rerio (Zebrafish) (13)

Drer\cd151l

7 of 15

Yes

Drer\cd151

5 of 15

Yes

Drer\tspan11

5 of 15

Yes

Drer\cd63

1 of 15

Drer\tspan3a

1 of 15

Yes

Drer\tspan3b

1 of 15

Yes

Drer\tspan12

1 of 15

Drer\tspan18a

1 of 15

Drer\tspan18b

1 of 15

Yes

Drer\tspan34

1 of 15

Yes

Drer\tspan35

1 of 15

Yes

Drer\tspan36

1 of 15

Yes

Drer\tspan37

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\tsp-15

3 of 15

Yes

Cele\tsp-16

2 of 15

Yes

Cele\tsp-20

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (4)

Atha\AT2G20230

1 of 9

Yes

Atha\AT2G20740

1 of 9

Yes

Atha\AT4G28770

1 of 9

Yes

Atha\TOM2A

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190E3M )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18924

Drosophila persimilis

Dper\GL22737

Drosophila willistoni

Dwil\GK20576

Drosophila virilis

Dvir\GJ14111

Drosophila mojavensis

Dmoj\GI13972

Drosophila grimshawi

Dgri\GH14575

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150AX1 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17021169

Glossina morsitans

Tsetse fly

Gmor\GMOY007608

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_13202

Mayetiola destructor

Hessian fly

Mdes\Mdes008522

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL005137

Anopheles darlingi

American malaria mosquito

Adar\ADAC001743

Anopheles gambiae

Malaria mosquito

Agam\AGAP011342

Anopheles gambiae

Malaria mosquito

Agam\AGAP011343

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ006782

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0FG8 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001039

Danaus plexippus

Monarch butterfly

Dple\DPOGS200740-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL008964

Apis florea

Little honeybee

Aflo\3009471028

Apis mellifera

Western honey bee

Amel\Tspan13

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP19622

Bombus terrestris

Buff-tailed bumblebee

Bter\15215043

Linepithema humile

Argentine ant

Lhum\LH14988

Linepithema humile

Argentine ant

Lhum\LH11334

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000ea8

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG020631

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07531

Pediculus humanus

Human body louse

Phum\PHUM339210

Rhodnius prolixus

Kissing bug

Rpro\RPRC009253

Cimex lectularius

Bed bug

Clec\CLEC012312

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008432

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04695

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0CBX )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Black-legged tick

Isca\ISCW019831

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58941.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58654.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur06g00890

Tetranychus urticae

Two-spotted spider mite

Turt\tetur13g00270

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_232717

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0JM9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ttrspn_16

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ttrspn_12

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ttrspn_15

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_007578gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ttrspn_4

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ttrspn_7

Ciona intestinalis

Vase tunicate

Cint\LOC100184001

Ciona intestinalis

Vase tunicate

Cint\LOC100177116

Ciona intestinalis

Vase tunicate

Cint\LOC100185551

Ciona intestinalis

Vase tunicate

Cint\LOC100185498

Ciona intestinalis

Vase tunicate

Cint\LOC100179718

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (33)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

lbm

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CD151; CD151 molecule (Raph blood group)

7 of 15

602243

TSPAN11; tetraspanin 11

6 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Tsp66E|Tsp74F

(Han et al., 2012)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

65280

DroID - A comprehensive database of gene and protein interactions.

FBgn0036769

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-45

Cytogenetic map

75A8-75A8

Sequence location

3L:17,891,820..17,895,862 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

75A8-75A8

Limits computationally determined from genome sequence between P{lacW}l(3)j11B2^j11B2 and P{PZ}W⁰⁵⁰¹⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-41.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Bloomington

62573

w¹¹¹⁸; PBac{IT.GAL4}Tsp74F^0006-G4

58579

y¹ w^*; Mi{MIC}Tsp74F^MI13017

15729

y¹ w^67c23; P{EPgy2}Tsp74F^EY04256

39046

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01966}attP40

VDRC

v330520

P{VSH330520}attP40

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (84)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE29825

Other clones

DPiM_RE29825

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0036769

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0036769

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39995

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Tsp74F CG5492

(Todres et al., 2000)

Source for database merge of

Additional comments

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 32 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39995

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q7JZI2

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036769

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0036769

DRscDB

39995/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0036769

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Tsp74F_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5492

Flygut - An atlas of the Drosophila adult midgut

FBgn0036769

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39995

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007531

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5492

MARRVEL_MODEL

39995

modMine - A data warehouse for the modENCODE project

FBgn0036769

Linkouts

BioGRID - A database of protein and genetic interactions.

65280

DroID - A comprehensive database of gene and protein interactions.

FBgn0036769

DRSC - Results frm RNAi screens

FBgn0036769

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5492-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0036769

FlyMine - An integrated database for Drosophila genomics

FBgn0036769

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG5492

(Meadows, 2018.4.4, Ni et al., 2010.12.1, Fradkin et al., 2002, Seigneuret et al., 2001, Todres et al., 2000)

Dm.Tsp74F

(Todres et al., 2000)

Tsp74F

(Grobler et al., 2018, Prudêncio et al., 2018, Fu et al., 2017, Clandinin and Owens, 2016-, Jeibmann et al., 2015, Han et al., 2012)

tsp74F

(González-Méndez et al., 2020)

Name Synonyms

Tetraspanin 74F

(Peiris et al., 2018, Podolsky et al., 2018, Todres et al., 2000)

Tetraspanin74F

(Jeibmann et al., 2015)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (37)

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