FB2020_01, released Feb 12, 2020

Gene: Dmel\Lon

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General Information
Symbol
Dmel\Lon
Species
D. melanogaster
Name
Lon protease
Annotation Symbol
CG8798
Feature Type
protein_coding_gene
FlyBase ID
FBgn0036892
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Snapshot
Lon protease (Lon) encodes a conserved ATP-stimulated serine protease. It is encoded in the nucleus and targeted to the mitochondrial matrix, where it contributes to mitochondrial protein turnover. [Date last reviewed: 2018-10-25]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
76B9-76B9
Sequence location
3L:19,607,857..19,612,199 [-]
Recombination map

3-45

RefSeq locus
NT_037436 REGION:19607857..19612199
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the peptidase S16 family. (Q7KUT2)
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
ATP binding; ATP-dependent peptidase activity; serine-type endopeptidase activity; single-stranded DNA binding
Summaries
Protein Function (UniProtKB)
ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner. Regulates mitochondrial DNA (mtDNA) copy number and transcription by stabilizing the mitochondrial TFAM:mtDNA ratio via selective degradation of TFAM.
(UniProt, Q7KUT2)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Lon or the JBrowse view of Dmel\Lon for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Lon-RA
FBtr0074967
NM_140876
3707
1006
Lon-RC
FBtr0273410
NM_168806
3761
1024
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Lon-PA
FBpp0074735
112.9
1006
7.32
NP_649133
AAF49134
Lon-PC
FBpp0271918
115.0
1024
7.66
NP_730435
AAN11654
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Homohexamer or homoheptamer. Organized in a ring with a central cavity.

(UniProt, Q7KUT2)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Lon using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (11 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
ATP binding
inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR004815, InterPro:IPR027065
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
ATP-dependent peptidase activity
inferred from biological aspect of ancestor with PANTHER:PTN002464028
(assigned by GO_Central )
(Gaudet et al., 2011)
serine-type endopeptidase activity
inferred from sequence or structural similarity with UniProtKB:P36776
(Sardiello et al., 2003.6.11)
single-stranded DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000005479
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
mitochondrion organization
inferred from mutant phenotype
(Matsushima et al., 2010)
positive regulation of protein catabolic process
inferred from mutant phenotype
(Thomas et al., 2014)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
chaperone-mediated protein complex assembly
inferred from biological aspect of ancestor with PANTHER:PTN000005479
(assigned by GO_Central )
(Gaudet et al., 2011)
mitochondrion organization
inferred from biological aspect of ancestor with PANTHER:PTN000005479
(assigned by GO_Central )
(Gaudet et al., 2011)
protein quality control for misfolded or incompletely synthesized proteins
inferred from biological aspect of ancestor with PANTHER:PTN002464028
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
mitochondrion
inferred from direct assay
(Matsushima et al., 2010)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
mitochondrial matrix
inferred from biological aspect of ancestor with PANTHER:PTN000005479
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with UniProtKB:P36776
(Sardiello et al., 2003.6.11)
peroxisome
inferred from sequence or structural similarity with HGNC:20598
(Faust et al., 2012)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
mitochondrion
inferred from direct assay
(Matsushima et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Lon in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Lon
Transgenic constructs containing regulatory region of Lon
Deletions and Duplications ( 4 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\LONP1
15 of 15
Yes
Yes
 
Hsap\LONP2
3 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Lonp1
14 of 15
Yes
Yes
Mmus\Lonp2
3 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
Rnor\Lonp1
11 of 13
Yes
Yes
Rnor\Lonp2
3 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\lonp1
8 of 12
Yes
Yes
Xtro\lonp2
1 of 12
No
Yes
Danio rerio (Zebrafish) (3)
Drer\lonp1
14 of 15
Yes
Yes
Drer\lonp2
2 of 15
No
Yes
Drer\b4galnt3a
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\lonp-1
13 of 15
Yes
Yes
Cele\lonp-2
2 of 15
No
Yes
Cele\C35B1.4
1 of 15
No
Yes
Cele\T23G4.2
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (5)
Atha\LON1
9 of 9
Yes
Yes
Atha\LON3
8 of 9
No
Yes
Atha\LON4
7 of 9
No
Yes
Atha\LON2
2 of 9
No
Yes
Atha\AT3G05775
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\PIM1
15 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\lon1
12 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919023C )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\Lon
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00010983
Drosophila simulans
Dsim\GD12266
Drosophila sechellia
Dsec\GM17358
Drosophila erecta
Dere\GG13389
Drosophila yakuba
Dyak\GE22482
Drosophila yakuba
Dyak\GE22769
Drosophila yakuba
Dyak\GE28813
Drosophila ananassae
Dana\GF10722
Drosophila pseudoobscura pseudoobscura
Dpse\GA26898
Drosophila pseudoobscura pseudoobscura
Dpse\GA21329
Drosophila persimilis
Dper\GL13934
Drosophila persimilis
Dper\GL20881
Drosophila willistoni
Dwil\GK20242
Drosophila virilis
Dvir\GJ11872
Drosophila mojavensis
Dmoj\GI13511
Drosophila grimshawi
Dgri\GH14623
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915014S )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17014402
Glossina morsitans
Tsetse fly
Gmor\GMOY005933
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_08334
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL006474
Anopheles darlingi
American malaria mosquito
Adar\ADAC000345
Anopheles gambiae
Malaria mosquito
Agam\AGAP010451
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ008800
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00UK )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA007194
Danaus plexippus
Monarch butterfly
Dple\DPOGS215092-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL010543
Apis florea
Little honeybee
Aflo\2453769636
Apis mellifera
Western honey bee
Amel\LOC409459
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP24724
Bombus terrestris
Buff-tailed bumblebee
Bter\15214442
Linepithema humile
Argentine ant
Lhum\LH18830
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00133c
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG007388
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08492
Tribolium castaneum
Red flour beetle
Tcas\TC000570
Pediculus humanus
Human body louse
Phum\PHUM244850
Rhodnius prolixus
Kissing bug
Rpro\RPRC011672
Cimex lectularius
Bed bug
Clec\CLEC008158
Cimex lectularius
Bed bug
Clec\CLEC006122
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI003409
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_12656
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_16353
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01IA )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR012030
Ixodes scapularis
Black-legged tick
Isca\ISCW000683
Stegodyphus mimosarum
African social velvet spider
Smim\KFM59256.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur24g01180
Tetranychus urticae
Two-spotted spider mite
Turt\tetur07g07460
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_306039
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_259078
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01TN )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Lon
Ciona intestinalis
Vase tunicate
Cint\LOC100178941
Gallus gallus
Domestic chicken
Ggal\LONP1
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
LonGD14030
model of  CODAS syndrome
CEA
(Pareek et al., 2018)
Potential Models Based on Orthology ( 1 )
Human Ortholog
Disease
Evidence
References
LONP1; lon peptidase 1, mitochondrial
model of  CODAS syndrome
IEA
(FlyBase, 2019-)
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
LonGD14030
model of  CODAS syndrome
is ameliorated by ClpPUAS.Tag:FLAG,Tag:HA
(Pareek et al., 2018)
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
LONP1; lon peptidase 1, mitochondrial
15 of 15
605490
 
LONP2; lon peptidase 2, peroxisomal
3 of 15
617774
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser
      View in Interactions Browser
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Lon - Akt1
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Lon - Nrd1
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Yoon et al., 2017)
      Lon - ex
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Lon
      suppressible
      ClpP
      (Pareek et al., 2018)
      Lon
      enhanceable
      ClpP
      (Pareek et al., 2018)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Nsf2
      suppressible
      Lon
      (Laviolette et al., 2005)
      Pink1
      enhanceable
      Lon
      (Thomas et al., 2014)
      Pink1
      suppressible
      Lon
      (Thomas et al., 2014)
      External Data
      Subunit Structure (UniProtKB)
      Homohexamer or homoheptamer. Organized in a ring with a central cavity.
      (UniProt, Q7KUT2 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map

      3-45

      Cytogenetic map
      76B9-76B9
      Sequence location
      3L:19,607,857..19,612,199 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      76B9-76B9
      Limits computationally determined from genome sequence between P{EP}MESR6EP3142 and P{lacW}l(3)L3809L3809
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      3-45

      (FlyBase, 2016)

      3-43.1

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (10)
      Genomic Clones (15)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (22)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Matsushima et al., 2010)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: Lon CG8798

        (Matsushima et al., 2010)
        Source for database merge of

        Source for merge of: CG8798 anon-WO0118547.350

        (FlyBase, 1996-)
        Additional comments

        Source for merge of CG8798 anon-WO0118547.350 was sequence comparison ( date:051113 ).

        (FlyBase, 1996-)
        Other Comments

        Area matching Drosophila EST AA539054.

        (Davies, 2001.3.15)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 46 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (4)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (61)