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Gene Dmel\CG8786

General Information
Symbol	Dmel\CG8786	Species	D. melanogaster
Name		Annotation symbol	CG8786
Feature type	protein_coding_gene	FlyBase ID	FBgn0036897
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	76B11-76B11	Sequence location	3L:19,630,868..19,635,916 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG8786 (FBgn0036897). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: poly-ADP-D-ribose binding; ubiquitin-protein ligase activity. There is experimental evidence that it is involved in the biological process: protein destabilization. 4 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: WWE domain; WWE domain, subgroup; Zinc finger, RING-type; Zinc finger, RING-type, conserved site; Zinc finger, RING/FYVE/PHD-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval Malpighian tubules, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1639950_at completely aligns to an exonic region of only a subset of 2 of the 4 FlyBase-annotated transcript isoforms of CG8786. Gene sequence location is 3L:19630868..19635916.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 76B11-76B11   Limits computationally determined from genome sequence between P{lacW}l(3)L3809L3809 and P{lacW}l(3)L1243L1243&P{lacW}Mi-2j3D4
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG8786 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 Low-frequency RNA-seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG8786-RB FBtr0074962  NM_140881   1963   376 CG8786-RC FBtr0290116  NM_001144506   1667   448 CG8786-RF FBtr0306354  NM_001144508   1376   365 CG8786-RG FBtr0331862  NM_001144507   3301   448
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG8786-PB   FBpp0074730   39.5   376   4.43     NP_649138   AAF49128 CG8786-PC   FBpp0288555   47.8   448   4.25     NP_001137978   ACL83333 CG8786-PF   FBpp0297447   38.9   365   4.16     NP_001137980   ACL83335 CG8786-PG   FBpp0304246   47.8   448   4.25     NP_001137979   ACL83334
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • WWE domain, subgroup (IPR018123) Zinc finger, RING-type (IPR001841) WWE domain (IPR004170) Zinc finger, RING-type, conserved site (IPR017907) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA694780   AE014296 AAF49128   AE014296 ACL83333   AE014296 ACL83334   AE014296 ACL83335   AI259587   AI514611   AI546212   AI947247   AW941139   AY070592 AAL48063   AY121630 AAM51957   BI242884   BI486027   BI612447   BI613626   BI617671   BI626166   BP540792   BP557286   BT046135   BT081990   BT122073   CL526073   CO272040   CO294195   CO305321   CO315056   CO326247   CO330982   CO342588   EC083231   EC088424   EC194788   EC202946   EC229098   EC239519   EC253595   EC255588   GH757469   GH777359   GH780283   GH792467   GH809608   GH813078   GH816282   GH818743   GH819973   GH820071   GH820912   GH827679   GH829217   GH830256   GH837693   GH856044   GH857779   GH860616   GH861079   GH862274   GH864968   GH866050   GH872272   GH875093   GH875687   GH877452   GH879411   GH894910   GH903727   GH904627   GH907623   GH907888   GH925721   GH929601   GH936687
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VW25 B7Z078 B7Z083 Q8MRE3 Q8SZQ1 B7Z077
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval Malpighian tubules, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    171.35 Larval Midgut    60 Larval Hindgut    79.5 Larval Malpighian Tubules    142.3 Larval Fat Body    71.8 Larval Salivary Gland    155.8 Larval Trachea    184.65 Larval Carcass    106.625 Adult Head    99.6 Adult Eye    155.5 Adult Brain    202.7 Adult Thoracic-Abdominal Ganglion    191 Adult Crop    82.4 Adult Midgut    58.8 Adult Hindgut    66.8 Adult Malpighian Tubules    85.3 Adult Fat Body    52 Adult Salivary Gland    141.4 Adult Heart    62.8 Adult VirginFemale Spermatheca    88.3 Adult InseminatedFemale Spermatheca    113.6 Adult Ovary    310.6 Adult Testis    11.2 Adult Male Accessory Gland    114 Adult Carcass    43.3 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    171.35 Larval Midgut    60 Larval Hindgut    79.5 Larval Malpighian Tubules    142.3 Larval Fat Body    71.8 Larval Salivary Gland    155.8 Larval Trachea    184.65 Larval Carcass    106.625 Adult Head    99.6 Adult Eye    155.5 Adult Brain    202.7 Adult Thoracic-Abdominal Ganglion    191 Adult Crop    82.4 Adult Midgut    58.8 Adult Hindgut    66.8 Adult Malpighian Tubules    85.3 Adult Fat Body    52 Adult Salivary Gland    141.4 Adult Heart    62.8 Adult VirginFemale Spermatheca    88.3 Adult InseminatedFemale Spermatheca    113.6 Adult Ovary    310.6 Adult Testis    11.2 Adult Male Accessory Gland    114 Adult Carcass    43.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    171.35 Larval Midgut    60 Larval Hindgut    79.5 Larval Malpighian Tubules    142.3 Larval Fat Body    71.8 Larval Salivary Gland    155.8 Larval Trachea    184.65 Larval Carcass    106.625 Adult Head    99.6 Adult Eye    155.5 Adult Brain    202.7 Adult Thoracic-Abdominal Ganglion    191 Adult Crop    82.4 Adult Midgut    58.8 Adult Hindgut    66.8 Adult Malpighian Tubules    85.3 Adult Fat Body    52 Adult Salivary Gland    141.4 Adult Heart    62.8 Adult VirginFemale Spermatheca    88.3 Adult InseminatedFemale Spermatheca    113.6 Adult Ovary    310.6 Adult Testis    11.2 Adult Male Accessory Gland    114 Adult Carcass    43.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    171.35 Larval Midgut    60 Larval Hindgut    79.5 Larval Malpighian Tubules    142.3 Larval Fat Body    71.8 Larval Salivary Gland    155.8 Larval Trachea    184.65 Larval Carcass    106.625 Adult Head    99.6 Adult Eye    155.5 Adult Brain    202.7 Adult Thoracic-Abdominal Ganglion    191 Adult Crop    82.4 Adult Midgut    58.8 Adult Hindgut    66.8 Adult Malpighian Tubules    85.3 Adult Fat Body    52 Adult Salivary Gland    141.4 Adult Heart    62.8 Adult VirginFemale Spermatheca    88.3 Adult InseminatedFemale Spermatheca    113.6 Adult Ovary    310.6 Adult Testis    11.2 Adult Male Accessory Gland    114 Adult Carcass    43.3 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    171.35 Larval Midgut    60 Larval Hindgut    79.5 Larval Malpighian Tubules    142.3 Larval Fat Body    71.8 Larval Salivary Gland    155.8 Larval Trachea    184.65 Larval Carcass    106.625 Adult Head    99.6 Adult Eye    155.5 Adult Brain    202.7 Adult Thoracic-Abdominal Ganglion    191 Adult Crop    82.4 Adult Midgut    58.8 Adult Hindgut    66.8 Adult Malpighian Tubules    85.3 Adult Fat Body    52 Adult Salivary Gland    141.4 Adult Heart    62.8 Adult VirginFemale Spermatheca    88.3 Adult InseminatedFemale Spermatheca    113.6 Adult Ovary    310.6 Adult Testis    11.2 Adult Male Accessory Gland    114 Adult Carcass    43.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    171.35 Larval Midgut    60 Larval Hindgut    79.5 Larval Malpighian Tubules    142.3 Larval Fat Body    71.8 Larval Salivary Gland    155.8 Larval Trachea    184.65 Larval Carcass    106.625 Adult Head    99.6 Adult Eye    155.5 Adult Brain    202.7 Adult Thoracic-Abdominal Ganglion    191 Adult Crop    82.4 Adult Midgut    58.8 Adult Hindgut    66.8 Adult Malpighian Tubules    85.3 Adult Fat Body    52 Adult Salivary Gland    141.4 Adult Heart    62.8 Adult VirginFemale Spermatheca    88.3 Adult InseminatedFemale Spermatheca    113.6 Adult Ovary    310.6 Adult Testis    11.2 Adult Male Accessory Gland    114 Adult Carcass    43.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    171.35 Larval Midgut    60 Larval Hindgut    79.5 Larval Malpighian Tubules    142.3 Larval Fat Body    71.8 Larval Salivary Gland    155.8 Larval Trachea    184.65 Larval Carcass    106.625 Adult Head    99.6 Adult Eye    155.5 Adult Brain    202.7 Adult Thoracic-Abdominal Ganglion    191 Adult Crop    82.4 Adult Midgut    58.8 Adult Hindgut    66.8 Adult Malpighian Tubules    85.3 Adult Fat Body    52 Adult Salivary Gland    141.4 Adult Heart    62.8 Adult VirginFemale Spermatheca    88.3 Adult InseminatedFemale Spermatheca    113.6 Adult Ovary    310.6 Adult Testis    11.2 Adult Male Accessory Gland    114 Adult Carcass    43.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    171.35 Larval Midgut    60 Larval Hindgut    79.5 Larval Malpighian Tubules    142.3 Larval Fat Body    71.8 Larval Salivary Gland    155.8 Larval Trachea    184.65 Larval Carcass    106.625 Adult Head    99.6 Adult Eye    155.5 Adult Brain    202.7 Adult Thoracic-Abdominal Ganglion    191 Adult Crop    82.4 Adult Midgut    58.8 Adult Hindgut    66.8 Adult Malpighian Tubules    85.3 Adult Fat Body    52 Adult Salivary Gland    141.4 Adult Heart    62.8 Adult VirginFemale Spermatheca    88.3 Adult InseminatedFemale Spermatheca    113.6 Adult Ovary    310.6 Adult Testis    11.2 Adult Male Accessory Gland    114 Adult Carcass    43.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    99.6   NA  Eye    155.5   NA  Brain    202.7   171.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    191   NA  Crop    82.4   60  Midgut    58.8   79.5  Hindgut    66.8   142.3  Malpighian Tubules    85.3   71.8  Fat Body    52   155.8  Salivary Gland    141.4   NA  Heart    62.8   184.65  Trachea    NA   NA  VirginFemale Spermatheca    88.3   NA  InseminatedFemale Spermatheca    113.6   NA  Ovary    310.6   NA  Testis    11.2   NA  Male Accessory Gland    114   106.625  Carcass    43.3 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    8 central nervous system, pupae P8    6 head, virgin 1-day female    5 head, virgin 4-day female    3 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    5 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    16 ovary, mated 4-day female    12 testis, mated 4-day male    33 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  26 central nervous system, larvae L3    8 central nervous system, pupae P8    6 head, virgin 1-day female    5 head, virgin 4-day female    3 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    5 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    16 ovary, mated 4-day female    12 testis, mated 4-day male  (33) accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    8 central nervous system, pupae P8    6 head, virgin 1-day female    5 head, virgin 4-day female    3 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    5 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    16 ovary, mated 4-day female    12 testis, mated 4-day male    33 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    8 central nervous system, pupae P8    6 head, virgin 1-day female    5 head, virgin 4-day female    3 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    5 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    16 ovary, mated 4-day female    12 testis, mated 4-day male    33 accessory gland, mated 4-day male    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    8 central nervous system, pupae P8    6 head, virgin 1-day female    5 head, virgin 4-day female    3 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    5 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    16 ovary, mated 4-day female    12 testis, mated 4-day male    33 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  26 central nervous system, larvae L3    8 central nervous system, pupae P8    6 head, virgin 1-day female    5 head, virgin 4-day female    3 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    5 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    16 ovary, mated 4-day female    12 testis, mated 4-day male  (33) accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    8 central nervous system, pupae P8    6 head, virgin 1-day female    5 head, virgin 4-day female    3 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    5 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    16 ovary, mated 4-day female    12 testis, mated 4-day male    33 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    8 central nervous system, pupae P8    6 head, virgin 1-day female    5 head, virgin 4-day female    3 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    5 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    5 carcass, larvae L3 wandering    6 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    16 ovary, mated 4-day female    12 testis, mated 4-day male    33 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    7 embryo 04-06hr    11 embryo 06-08hr    11 embryo 08-10hr    9 embryo 10-12hr    5 embryo 12-14hr    5 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    3 larva L2    2 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    11 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    10 adult male 30day    9 adult female 01day    5 adult female 05day    10 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    7 embryo 04-06hr    11 embryo 06-08hr    11 embryo 08-10hr    9 embryo 10-12hr    5 embryo 12-14hr    5 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    3 larva L2    2 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    11 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    10 adult male 30day    9 adult female 01day    5 adult female 05day    10 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    7 embryo 04-06hr    11 embryo 06-08hr    11 embryo 08-10hr    9 embryo 10-12hr    5 embryo 12-14hr    5 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    3 larva L2    2 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    11 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    10 adult male 30day    9 adult female 01day    5 adult female 05day    10 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    7 embryo 04-06hr    11 embryo 06-08hr    11 embryo 08-10hr    9 embryo 10-12hr    5 embryo 12-14hr    5 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    3 larva L2    2 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    11 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    10 adult male 30day    9 adult female 01day    5 adult female 05day    10 adult female 30day    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    7 embryo 04-06hr    11 embryo 06-08hr    11 embryo 08-10hr    9 embryo 10-12hr    5 embryo 12-14hr    5 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    3 larva L2    2 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    11 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    10 adult male 30day    9 adult female 01day    5 adult female 05day    10 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    7 embryo 04-06hr    11 embryo 06-08hr    11 embryo 08-10hr    9 embryo 10-12hr    5 embryo 12-14hr    5 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    3 larva L2    2 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    11 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    10 adult male 30day    9 adult female 01day    5 adult female 05day    10 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    7 embryo 04-06hr    11 embryo 06-08hr    11 embryo 08-10hr    9 embryo 10-12hr    5 embryo 12-14hr    5 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    3 larva L2    2 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    11 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    10 adult male 30day    9 adult female 01day    5 adult female 05day    10 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    7 embryo 04-06hr    11 embryo 06-08hr    11 embryo 08-10hr    9 embryo 10-12hr    5 embryo 12-14hr    5 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    3 larva L2    2 larva L3 12hr old    3 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    11 white prepupae 24hr    11 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    10 adult male 30day    9 adult female 01day    5 adult female 05day    10 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    12 embryonic Kc167    8 embryonic S1    6 embryonic S3    6 leg disc CME L1    10 wing disc CME-W2    11 wing disc ML-DmD8    8 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    5 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    10 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    12 embryonic Kc167    8 embryonic S1    6 embryonic S3    6 leg disc CME L1    10 wing disc CME-W2    11 wing disc ML-DmD8    8 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    5 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    10 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    12 embryonic Kc167    8 embryonic S1    6 embryonic S3    6 leg disc CME L1    10 wing disc CME-W2    11 wing disc ML-DmD8    8 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    5 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    10 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    12 embryonic Kc167    8 embryonic S1    6 embryonic S3    6 leg disc CME L1    10 wing disc CME-W2    11 wing disc ML-DmD8    8 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    5 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    10 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    12 embryonic Kc167    8 embryonic S1    6 embryonic S3    6 leg disc CME L1    10 wing disc CME-W2    11 wing disc ML-DmD8    8 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    5 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    10 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    12 embryonic Kc167    8 embryonic S1    6 embryonic S3    6 leg disc CME L1    10 wing disc CME-W2    11 wing disc ML-DmD8    8 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    5 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    10 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    12 embryonic Kc167    8 embryonic S1    6 embryonic S3    6 leg disc CME L1    10 wing disc CME-W2    11 wing disc ML-DmD8    8 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    5 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    10 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    9 embryonic 1182-4H    4 embryonic GM2    12 embryonic Kc167    8 embryonic S1    6 embryonic S3    6 leg disc CME L1    10 wing disc CME-W2    11 wing disc ML-DmD8    8 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    5 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    10 ovary fGS/OSS    10 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0036897 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8786-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 CG8786GD14028 viable CG8786EY09040 Sterility Allele fertile CG8786EY09040 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 CG8786GD14028 visible, with Scer\GAL4pnr-MD237 CG8786GD14028 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 CG8786GD14028 trichogen cell, with Scer\GAL4pnr-MD237 CG8786GD14028
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG8786 Class Mutagen Stocks Known lesion CG8786EY09040 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG8786 Class Mutagen Stocks Known lesion CG8786GD14028 in vitro construct - RNAi in vitro construct - regulatory fusion 3 Yes CG8786HMS02130 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG8786NIG.8786R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Exel6135 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14028} NA P{NIG.8786R} NA P{TRiP.HMS02130} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}CG8786EY09040 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
protein destabilization inferred from mutant phenotype (Zhang et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
poly-ADP-D-ribose binding inferred from sequence or structural similarity with human RNF146 (Zhang et al., 2011) ubiquitin-protein ligase activity inferred from sequence or structural similarity with human RNF146 (Zhang et al., 2011) zinc ion binding inferred from electronic annotation with InterPro:IPR001841, InterPro:IPR018123 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
protein ubiquitination involved in ubiquitin-dependent protein catabolic process inferred from sequence or structural similarity with human RNF146 (Zhang et al., 2011)
Cellular Component
CV term Evidence References
cytosol inferred from sequence or structural similarity with human RNF146 (Curators, 2008--)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG8786 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 65414 DroID - A comprehensive database of gene and protein interactions. • FBgn0036897 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40144
Orthologs
OrthoDB Orthologs (2) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6N8RRX Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6N8RRX)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG8786     Drosophila yakuba   Dyak\GE22766
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD12263 Drosophila sechellia Dsec\GM17033 Drosophila erecta Dere\GG13386 Drosophila yakuba Dyak\GE22479 Drosophila ananassae Dana\GF10719 Drosophila pseudoobscura pseudoobscura Dpse\GA28223 Drosophila persimilis Dper\GL20877 Drosophila willistoni Dwil\GK25803 Drosophila virilis Dvir\GJ11868 Drosophila mojavensis Dmoj\GI13507 Drosophila grimshawi Dgri\GH14620
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	17526 y1 w67c23; P{EPgy2}CG8786EY09040 40882 y1 sc* v1; P{TRiP.HMS02130}attP2
VDRC	v36028 w1118; P{GD14028}v36028 v36029 w1118; P{GD14028}v36029 v43533 w1118; P{GD14028}v43533
Genomic Clones ( 1 )
	BACR23N10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 66 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM01527 RE69393
Other clones	FI04505 MIP02988 MIP19690
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD46142 LD47727 LP03037 RE40365 RH41319 RH42827 RH48075 RH66715
Other clones	7796 118262 119327 193741 214281.38 214481.47 bs36g08 CK01.12A.D6 CK01.21A.A6 EK105711 EK206307 EK238858 EK248222 EK296826 EP03517 EP15153 FGA007H12 FGM308D10 RP003001833 RP003072009 RP003075163 RP003076803 RP003094043 RP003105561 RP003123840 RP003124362 RP003134750 RP003152244 RP003154387 RP003155038 RP003177365 RP003200707 RP003203034 RP003210224 RP003213731 RP003224919 RP003230665 RP003242442 RP003266992 RP003269151 RP003291260 RP003319552 RP003324473 RP003335802 RP003345436 RP003363353 RP003372252 RP003375863 RP003445095 RP003493126 RP003520688 RP003566291 RP003635576
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0036897 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40144
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG8786 BcDNA:GM01527 (FlyBase, 1996-)
Additional comments	Source for merge of CG8786 BcDNA:GM01527 was a shared cDNA (date:030728). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140881 NM_001144506 NM_001144507 NM_001144508 RefSeq (Proteins) • NP_649138 NP_001137978 NP_001137979 NP_001137980 DDBJ / EMBL / GenBank • AA694780 AAF49128 AAL48063 AAM51957 ACL83333 ACL83334 ACL83335 AI546212 AI947247 AW941139 AY070592 AY121630 BI486027 CL526073 Entrez Gene - A searchable database of RefSeq genes. • 40144 UniProtKB/TrEMBL • B7Z077 B7Z078 B7Z083 Q8MRE3 Q8SZQ1 Q9VW25
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • WWE domain, subgroup (IPR018123) Zinc finger, RING-type (IPR001841) WWE domain (IPR004170) Zinc finger, RING-type, conserved site (IPR017907) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Linkouts
	BioGRID - A database of protein and genetic interactions • 65414 DroID - A comprehensive database of gene and protein interactions. • FBgn0036897 DRSC - Results from RNAi screens. • FBgn0036897 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0036897 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8786-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0036897 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40144 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40144 modMine - Data generated by the modENCODE project. • FBgn0036897
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	BcDNA:GM01527   CG8786 (Cooper et al., 2010, Zhang et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Ni et al., 2010.12.1) DRNF146 (Zhang et al., 2011) RNF146 (Zhang et al., 2011)
Name Synonym
Secondary FlyBase IDs
	FBgn0063208
References ( 19 )
Research paper	Zhang et al., 2011, Nat. Cell Biol. 13(5): 623--629 RNF146 is a poly(ADP-ribose)-directed E3 ligase that regulates axin degradation and Wnt signalling. [FBrf0218772] Cooper et al., 2010, Genetics 185(3): 811--822 Molecular Genetic Analysis of Chd3 and Polytene Chromosome Region 76B-D in Drosophila melanogaster. [FBrf0211329] Foley and O'Farrell, 2004, PLoS Biol. 2(8): e203 Functional dissection of an innate immune response by a genome-wide RNAi screen. [FBrf0179207]
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Review	Aravind, 2001, Trends Biochem. Sci. 26(5): 273--275 The WWE domain: a common interaction module in protein ubiquitination and ADP ribosylation. [FBrf0182522]
Personal communication to FlyBase	Ni et al., 2010.12.1, A genome-scale shRNA resource for transgenic RNAi in Drosophila. A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0212437] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Oliver, 2001.8.16, Genome Res.: File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348). [FBrf0137492] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
FlyBase analysis	FlyBase Curators, 2010-, Gene Ontology (GO) annotation review. Gene Ontology (GO) annotation review. [FBrf0216433] Curators, 2008--, Assigning Gene Ontology (GO) terms by sequence similarity in FlyBase. Assigning Gene Ontology (GO) terms by sequence similarity in FlyBase. [FBrf0202953] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]
Stock list	Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY. RNAi stocks available from NIG-FLY. [FBrf0218457]