FlyBase Gene Report: Dmel\Usp32

General Information

Symbol

Dmel\Usp32

Species

D. melanogaster

Name

Ubiquitin specific protease 32

Annotation Symbol

CG8334

Feature Type

protein_coding_gene

FlyBase ID

FBgn0036913

Gene Model Status

Current

Stock Availability

6 publicly available

Enzyme Name (EC)

Ubiquitinyl hydrolase 1 (3.4.19.12)

Gene Summary

Deubiquitinating enzyme that acts as an inhibitor of mitophagy probably by counteracting the action of park. Possibly functions by hydrolyzing ubiquitin attached by park on target proteins, thereby reducing park's ability to drive mitophagy. (UniProt, M9PD06)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

76D3-76D3

Sequence location

3L:19,871,139..19,881,783 [+]

Recombination map

3-46

RefSeq locus

NT_037436 REGION:19871139..19881783

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables calcium ion binding

inferred from electronic annotation with InterPro:IPR002048

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables thiol-dependent deubiquitinase

inferred from biological aspect of ancestor with PANTHER:PTN001163216

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in protein deubiquitination

inferred from sequence or structural similarity with HGNC:12629

(FlyBase Curators, 2008--)

involved_in ubiquitin-dependent protein catabolic process

inferred from electronic annotation with InterPro:IPR018200

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN001163216

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

USP DEUBIQUITINASES

Protein Family (UniProt)

Belongs to the peptidase C19 family. USP20/USP33 subfamily. (M9PD06)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

Predictions / Assertions

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) (3.4.19.12)

Summaries

Gene Group (FlyBase)

USP DEUBIQUITINASES -: The Ubiquitin Specific Proteases (USP) is the largest sub-family of deubiquitinases. They are cysteine proteases and catalyze the removal of ubiquitin from ubiquitin chains and ubiquitinated proteins. USP deubiquitinases belong to MEROPS family C19. (Adapted from PMID:19626045).

Protein Function (UniProtKB)

(UniProt, M9PD06)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Usp32 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 6.32

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Usp32-RF

FBtr0479916

NM_001275145

6311

1779

Usp32-RG

FBtr0479917

NM_001275146

6254

1760

Usp32-RH

FBtr0479918

NM_001275147

6245

1757

Usp32-RI

FBtr0479919

NM_001275148

6302

1776

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Usp32-PF

FBpp0428248

199.0

1779

6.52

AGB94767

Usp32-PG

FBpp0428249

196.8

1760

6.42

AGB94768

Usp32-PH

FBpp0428250

196.4

1757

6.48

AGB94769

Usp32-PI

FBpp0428251

198.7

1776

6.58

AGB94770

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

M9PD06

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Usp32 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG8334_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Usp32 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Usp32

View exonic expression by tissue for Dmel\Usp32

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40169/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036913

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036913

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036913

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8334

Flygut - An atlas of the Drosophila adult midgut

FBgn0036913

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Usp32

Transgenic constructs containing regulatory region of Usp32

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Usp32^GD5871

viable, with Scer\GAL4^Mef2.PR

Usp32^GD5871

viable, with Scer\GAL4^pnr-MD237

Usp32^GD5871

viable, with Scer\GAL4^tin.CΔ4

Usp32^GD5871

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (23)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\USP32

11 of 15

Yes

Hsap\USP6

8 of 15

Yes

Hsap\USP4

2 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (17)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Usp32

11 of 15

Yes

Mmus\Usp4

2 of 15

Yes

Mmus\Usp11

2 of 15

Mmus\Usp15

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (11)

Rnor\Usp32

9 of 13

Yes

Rnor\Ggnbp1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (9)

Xtro\usp32

7 of 12

Yes

Xtro\usp15

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (13)

Drer\usp32

12 of 15

Yes

Drer\usp4

2 of 15

Yes

Drer\usp11

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\cyk-3

11 of 15

Yes

Cele\F07A11.4

2 of 15

Yes

Cele\H34C03.2

2 of 15

Yes

Cele\usp-33

2 of 15

Cele\usp-3

1 of 15

Cele\usp-50

1 of 15

Arabidopsis thaliana (thale-cress) (5)

Atha\UBP5

3 of 9

Yes

Atha\UBP8

3 of 9

Yes

Atha\UBP9

2 of 9

Yes

Atha\UBP10

2 of 9

Yes

Atha\UBP11

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\UBP12

5 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\ubp1

4 of 12

Yes

Spom\ubp12

4 of 12

Yes

Spom\ubp4

2 of 12

Spom\ubp8

1 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900EQ )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20997

Drosophila persimilis

Dper\GL22665

Drosophila persimilis

Dper\GL22664

Drosophila willistoni

Dwil\GK19705

Drosophila virilis

Dvir\GJ11329

Drosophila mojavensis

Dmoj\GI11648

Drosophila grimshawi

Dgri\GH14664

Drosophila grimshawi

Dgri\GH17697

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500KA )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17018031

Glossina morsitans

Tsetse fly

Gmor\GMOY011234

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09308

Mayetiola destructor

Hessian fly

Mdes\Mdes007434

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL010184

Anopheles darlingi

American malaria mosquito

Adar\ADAC009231

Anopheles gambiae

Malaria mosquito

Agam\AGAP001209

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ010349

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W019M )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA013217

Bombyx mori

Silkmoth

Bmor\BGIBMGA013218

Danaus plexippus

Monarch butterfly

Dple\DPOGS212771-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL014157

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_11715

Tribolium castaneum

Red flour beetle

Tcas\TC033410

Pediculus humanus

Human body louse

Phum\PHUM322070

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI56628

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_09926

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X017O )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Black-legged tick

Isca\ISCW007961

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78271.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_225412

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01VD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Usp32_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Usp32_2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Usp32

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000002354

Gallus gallus

Domestic chicken

Ggal\USP32

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

not

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Usp32^GD5871

ameliorates Parkinson's disease

modeled by park^GD5543

(Cornelissen et al., 2014)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

USP32; ubiquitin specific peptidase 32

11 of 15

607740

USP6; ubiquitin specific peptidase 6

8 of 15

604334

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

park

suppressible

Usp32

(Cornelissen et al., 2014)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

65437

DroID - A comprehensive database of gene and protein interactions.

FBgn0036913

MIST (protein-protein) - An integrated Molecular Interaction Database

Usp32

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-46

Cytogenetic map

76D3-76D3

Sequence location

3L:19,871,139..19,881,783 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

76D3-76D3

Limits computationally determined from genome sequence between P{lacW}l(3)L3809^L3809 and P{lacW}l(3)L1243^L1243&P{lacW}Mi-2^j3D4

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-46

(FlyBase, 2016)

3-43.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Bloomington

29212

w¹¹¹⁸; Mi{ET1}Usp32^MB11462

61871

y¹ v¹; P{TRiP.HMJ23360}attP40

82076

y¹ sc^* v¹ sev²¹; P{TKO.GS04553}attP40

VDRC

v18981

w¹¹¹⁸; P{GD5871}v18981/TM3

v18982

w¹¹¹⁸; P{GD5871}v18982/TM3

v341836

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00991}attP40/CyO-GFP

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (14)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0036913

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0036913

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40169

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Usp32 CG8334

(Engel et al., 2014)

Source for database merge of

Additional comments

Other Comments

Origin and Etymology

Discoverer

Etymology

Named 'Usp32' based on its similarity to the human protein.

(Engel et al., 2014)

Identification

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40169

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

BI141930

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

M9PD06

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0036913

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40169/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036913

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG8334_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036913

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036913

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8334

Flygut - An atlas of the Drosophila adult midgut

FBgn0036913

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40169

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC033410

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8334

MARRVEL_MODEL - MARRVEL (model organism gene)

40169

modMine - A data warehouse for the modENCODE project

FBgn0036913

Linkouts

BioGRID - A database of protein and genetic interactions.

65437

DroID - A comprehensive database of gene and protein interactions.

FBgn0036913

DRSC - Results frm RNAi screens

FBgn0036913

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0036913

FlyMine - An integrated database for Drosophila genomics

FBgn0036913

MIST (protein-protein) - An integrated Molecular Interaction Database

Usp32

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG8334

(Badmos et al., 2021, Koltun et al., 2017, Lee et al., 2016, Von Stockum et al., 2016, Zhou et al., 2015, Cornelissen et al., 2014, Engel et al., 2014, Tsou et al., 2012, Zhang et al., 2012, Cooper et al., 2010, Perkins et al., 2009)

Q9VW49

(Clague et al., 2013)

USP15

(Cornelissen et al., 2014)

Usp32

(FlyBase Genome Annotators, 2019-, Transgenic RNAi Project members, 2017-, Engel et al., 2014)

dUSP15

(Cornelissen et al., 2018)

usp32

(Ristic et al., 2016)

Name Synonyms

Ubiquitin specific protease 32

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (38)

Report Sections

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