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Gene: Dmel\olf413

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General Information
Symbol
Dmel\olf413
Species
D. melanogaster
Name
olf413
Annotation Symbol
CG12673
Feature Type
protein_coding_gene
FlyBase ID
FBgn0037153
Gene Model Status
Current
Stock Availability
23 publicly available
Enzyme Name (EC)
Dopamine beta-monooxygenase (1.14.17.1)
Gene Snapshot
Insufficient genetic data for FlyBase to solicit a summary. [Date last reviewed: 2016-06-30]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
79C2-79D2
Sequence location
3L:22,136,090..22,247,702 [+]
Recombination map
3-47
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI Genome Data Viewer
UCSC Genome Browser
Ensembl Genome Browser
PopFly Genome Browser
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the copper type II ascorbate-dependent monooxygenase family. (Q6NP60)
Protein Domains/Motifs
InterPro
Copper type II, ascorbate-dependent monooxygenase, N-terminal; DOMON domain; PHM/PNGase F domain superfamily; Copper type II, ascorbate-dependent monooxygenase-like, C-terminal; Copper type II ascorbate-dependent monooxygenase, C-terminal; Tyramine beta-hydroxylase/Dopamine beta-hydroxylase; Copper type II, ascorbate-dependent monooxygenase, N-terminal domain superfamily
Molecular Function (see GO section for details)
Experimental Evidence
-
Predictions / Assertions
copper ion binding; dopamine beta-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
3,4-dihydroxyphenethylamine + ascorbate + O(2) = noradrenaline + dehydroascorbate + H(2)O (1.14.17.1)
Summaries
Gene Group Membership
PAIRED DONOR OXIDOREDUCTASES, INCORPORATION OF MOLECULAR OXYGEN, ASCORBATE AS DONOR -
Paired donor oxidoreductases, incorporation of molecular oxygen, ascorbate as donor include, dehydrogenases that catalyze an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from reduced ascorbate and one other donor, and one atom of oxygen is incorporated into one donor.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\olf413 or the JBrowse view of Dmel\olf413 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.53
Gene model reviewed during 5.46
Low-frequency RNA-Seq exon junction(s) not annotated.
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 5.56
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
olf413-RB
FBtr0301492
NM_168948
3443
760
olf413-RC
FBtr0333219
NM_001275275
8353
760
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
olf413-PB
FBpp0290707
84.7
760
8.10
NP_730711
AAF51810
olf413-PC
FBpp0305421
84.7
760
8.10
NP_001262204
AGB94897
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

760 aa isoforms: olf413-PB, olf413-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\olf413 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (11 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
copper ion binding
inferred from biological aspect of ancestor with PANTHER:PTN001605435
(assigned by GO_Central )
(Gaudet et al., 2011)
dopamine beta-monooxygenase activity
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen
inferred from biological aspect of ancestor with PANTHER:PTN001605435
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
dopamine catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
norepinephrine biosynthetic process
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
octopamine biosynthetic process
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
oxidation-reduction process
inferred from electronic annotation with InterPro:IPR000323, InterPro:IPR000945, InterPro:IPR014784
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN001605435
(assigned by GO_Central )
(Gaudet et al., 2011)
extracellular space
inferred from sequence or structural similarity with FLYBASE:to; FB:FBgn0039298
(Dauwalder et al., 2002)
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
secretory granule membrane
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
• membrane
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
Expression Deduced from Reporters
Reporter: PBac{681.P.FSVS-1}olf413CPTI001636
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult brain cell body rind
(Knowles-Barley, 2011.8.24)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\olf413 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 21 )
Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of olf413
Allele of olf413
Mutagen
Associated Transgenic Construct
Stocks
olf413JF02439
1
olf413KK103978
1
olf413GD3619
0
Transgenic constructs containing regulatory region of olf413
Deletions and Duplications ( 4 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\MOXD1
12 of 15
Yes
Yes
Hsap\DBH
3 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Moxd1
12 of 15
Yes
Yes
Mmus\Moxd2
5 of 15
No
Yes
Mmus\Dbh
2 of 15
No
No
Rattus norvegicus (Norway rat) (3)
Rnor\Moxd1
8 of 13
Yes
No
Rnor\Moxd2
6 of 13
No
Yes
Rnor\Dbh
2 of 13
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\moxd1
7 of 12
Yes
Yes
Xtro\dbh
2 of 12
No
No
Xtro\LOC105945848
1 of 12
No
Yes
Danio rerio (Zebrafish) (3)
Drer\moxd1
10 of 15
Yes
No
Drer\moxd1l
5 of 15
No
No
Drer\dbh
3 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\tbh-1
5 of 15
Yes
No
Cele\F41D9.2
1 of 15
No
Yes
Cele\Y54E10A.17
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No orthologs reported.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No orthologs reported.
Schizosaccharomyces pombe (Fission yeast) (0)
No orthologs reported.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091903LQ )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\olf413
Drosophila simulans
Dsim\GD15022
Drosophila sechellia
Dsec\GM22434
Drosophila erecta
Dere\GG16246
Drosophila yakuba
Dyak\GE22605
Drosophila ananassae
Dana\GF10948
Drosophila pseudoobscura pseudoobscura
Dpse\GA11749
Drosophila persimilis
Dper\GL25426
Drosophila willistoni
Dwil\GK17089
Drosophila virilis
Dvir\GJ13583
Drosophila mojavensis
Dmoj\GI13780
Drosophila grimshawi
Dgri\GH14670
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915029D )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17013515
Musca domestica
House fly
Mdoa\17013221
Musca domestica
House fly
Mdoa\17007358
Glossina morsitans
Tsetse fly
Gmor\GMOY002405
Glossina morsitans
Tsetse fly
Gmor\GMOY003133
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_10974
Mayetiola destructor
Hessian fly
Mdes\Mdes009131
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011332
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL008483
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL013836
Anopheles darlingi
American malaria mosquito
Adar\ADAC006294
Anopheles darlingi
American malaria mosquito
Adar\ADAC006292
Anopheles gambiae
Malaria mosquito
Agam\AGAP011609
Anopheles gambiae
Malaria mosquito
Agam\AGAP011613
Anopheles gambiae
Malaria mosquito
Agam\AGAP011618
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ014202
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ004736
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ006814
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01TL )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Apis florea
Little honeybee
Aflo\2147717315
Apis mellifera
Western honey bee
Amel\GB40694
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP20131
Bombus terrestris
Buff-tailed bumblebee
Bter\15205967
Linepithema humile
Argentine ant
Lhum\LH14013
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000a05
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG012863
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_02676
Tribolium castaneum
Red flour beetle
Tcas\TC014330
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_15706
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01RB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Stegodyphus mimosarum
African social velvet spider
Smim\KFM72379.1
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_62540
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02PG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000023424
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    Complementation?
    Transgene?
    MOXD1; monooxygenase DBH like 1
    12 of 15
    609000
      DBH; dopamine beta-hydroxylase
      3 of 15
      609312
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Interactions Browser
      Summary of Genetic Interactions
      esyN Network Diagram
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      Pathways
      Gene Group - Pathway Membership (FlyBase)
      External Data
      Linkouts
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map
      3-47
      Cytogenetic map
      79C2-79D2
      Sequence location
      3L:22,136,090..22,247,702 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      79C2-79D2
      Limits computationally determined from genome sequence between P{lacW}l(3)j1B10j1B10 and P{lacW}l(3)j2C4j2C4
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      79C-79D
      (Tickoo, 2000.4.13)
      Experimentally Determined Recombination Data
      Location
      3-47
      (FlyBase, 2016)
      3-44.0
      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (23)
      Genomic Clones (60)
      cDNA Clones (18)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for identity of: olf413 CG12673
          (FlyBase, 1996-)
          Source for database merge of
          Source for merge of: olf413 CG7495 CG14461
          (FlyBase Genome Annotators, 2004, FlyBase Genome Annotators, 2002-2003)
          Additional comments
          Source for identity of olf413 CG12673 was sequence comparison ( date:001001 ).
          (FlyBase Genome Annotators, 2004, FlyBase, 1996-)
          Annotations CG12673, CG7495 and CG14461 merged as CG12673 in release 3 of the genome annotation.
          (FlyBase Genome Annotators, 2002-2003)
          Other Comments
          New annotation (CR33185) in release 3 of the genome annotation.
          (FlyBase Genome Annotators, 2002-2003)
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 48 )
          Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          InterPro - A database of protein families, domains and functional sites
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc - Genes from a BioCyc PGDB for Dmel
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          FlyMine - An integrated database for Drosophila genomics
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          SignaLink - A signaling pathway resource with multi-layered regulatory networks.
          Synonyms and Secondary IDs (6)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (47)