FB2022_03, released June 9, 2022

Gene: Dmel\olf413

Open Close
General Information
Symbol
Dmel\olf413
Species
D. melanogaster
Name
olf413
Annotation Symbol
CG12673
Feature Type
protein_coding_gene
FlyBase ID
FBgn0037153
Gene Model Status
Current
Stock Availability
12 publicly available
Enzyme Name (EC)
Dopamine beta-monooxygenase (1.14.17.1)
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
79C2-79D2
Sequence location
Recombination map
3-47
RefSeq locus
NT_037436 REGION:22136090..22247702
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables copper ion binding
inferred from biological aspect of ancestor with PANTHER:PTN001605435
(assigned by GO_Central )
(Gaudet et al., 2011)
enables dopamine beta-monooxygenase activity
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in dopamine catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in norepinephrine biosynthetic process
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in octopamine biosynthetic process
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in extracellular space
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in extracellular space
inferred from sequence or structural similarity with FLYBASE:to; FB:FBgn0039298
(Dauwalder et al., 2002)
is_active_in secretory granule membrane
inferred from biological aspect of ancestor with PANTHER:PTN000806665
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the copper type II ascorbate-dependent monooxygenase family. (Q6NP60)
Protein Signatures (InterPro)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
3,4-dihydroxyphenethylamine + 2 ascorbate + O(2) = noradrenaline + 2 monodehydroascorbate + H(2)O (1.14.17.1)
Summaries
Gene Group (FlyBase)
MONOOXYGENASES, REDUCED ASCORBATE AS ONE DONOR -
Monooxygenases, reduced ascorbate as one donor include dehydrogenases that catalyze an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from reduced ascorbate and one other donor, and one atom of oxygen is incorporated into one donor.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\olf413 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q6NP60)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.53

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
olf413-RB
FBtr0301492
NM_168948
3443
760
olf413-RC
FBtr0333219
NM_001275275
8353
760
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
olf413-PB
FBpp0290707
84.7
760
8.10
Q6NP60
NP_730711
AAF51810
olf413-PC
FBpp0305421
84.7
760
8.10
Q6NP60
NP_001262204
AGB94897
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

760 aa isoforms: olf413-PB, olf413-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\olf413 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:
 low
high 
Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.
Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:
 low
high 
as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
Expression Deduced from Reporters
Reporter: PBac{681.P.FSVS-1}olf413CPTI001636
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult brain cell body rind
(Knowles-Barley, 2011.8.24)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\olf413 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 21 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of olf413
Transgenic constructs containing regulatory region of olf413
Aberrations (Deficiencies and Duplications) ( 4 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\MOXD1
10 of 15
Yes
No
Hsap\DBH
2 of 15
No
No
Hsap\MOXD2P
2 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Moxd1
11 of 15
Yes
No
Mmus\Moxd2
5 of 15
No
Yes
Mmus\Dbh
1 of 15
No
No
Rattus norvegicus (Norway rat) (3)
Rnor\Moxd1
8 of 13
Yes
No
Rnor\Moxd2
6 of 13
No
Yes
Rnor\Dbh
2 of 13
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\moxd1
8 of 12
Yes
Yes
Xtro\dbh
2 of 12
No
No
Xtro\LOC105945848
1 of 12
No
Yes
Danio rerio (Zebrafish) (3)
Drer\moxd1
9 of 15
Yes
No
Drer\moxd1l
5 of 15
No
No
Drer\dbh
2 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\tbh-1
4 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (2)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG5235
6 of 10
Tbh
4 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MOXD1; monooxygenase DBH like 1
    10 of 15
    609000
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Interactions Browser
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3L
        Recombination map
        3-47
        Cytogenetic map
        79C2-79D2
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        79C2-79D2
        Limits computationally determined from genome sequence between P{lacW}l(3)j1B10j1B10 and P{lacW}l(3)j2C4j2C4
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        79C-79D
        (Tickoo, 2000.4.13)
        Experimentally Determined Recombination Data
        Location

        3-47

        (FlyBase, 2016)

        3-44.0

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (12)
        Genomic Clones (60)
        cDNA Clones (18)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: olf413 CG12673

            (FlyBase, 1996-)
            Source for database merge of

            Source for merge of: olf413 CG7495 CG14461

            (FlyBase Genome Annotators, 2004, FlyBase Genome Annotators, 2002-2003)
            Additional comments

            Source for identity of olf413 CG12673 was sequence comparison ( date:001001 ).

            (FlyBase Genome Annotators, 2004, FlyBase, 1996-)

            Annotations CG12673, CG7495 and CG14461 merged as CG12673 in release 3 of the genome annotation.

            (FlyBase Genome Annotators, 2002-2003)
            Other Comments

            New annotation (CR33185) in release 3 of the genome annotation.

            (FlyBase Genome Annotators, 2002-2003)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 56 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            modMine - A data warehouse for the modENCODE project
            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Synonyms and Secondary IDs (6)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            References (53)