Gene Dmel\CG12582

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General Information

Symbol

Dmel\CG12582

Species

D. melanogaster

Name

Annotation symbol

CG12582

Feature type

protein_coding_gene

FlyBase ID

FBgn0037215

Gene Model Status

Current

Stock availability

3 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

81F6-81F6

Sequence location

3R:22,928..30,294 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG12582 (FBgn0037215). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: beta-mannosidase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: carbohydrate metabolic process. 4 alleles are reported. No phenotypic data is available. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Galactose-binding domain-like; Glycoside hydrolase, catalytic domain; Glycoside hydrolase, family 2/20, immunoglobulin-like beta-sandwich domain; Glycoside hydrolase, superfamily. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult midgut, adult Malpighian tubules, adult salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1624198_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of CG12582. Gene sequence location is 3R:22928..30294.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

81F6-81F6

Limits computationally determined from genome sequence between P{PZ}l(3)02733⁰²⁷³³ and P{EP}EP974

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG12582 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.39

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG12582-RA

FBtr0078964

NM_141179

3035

908

CG12582-RD

FBtr0306022

NM_001259970

2922

908

CG12582-RF

FBtr0336702

NM_164315

3100

778

CG12582-RG

FBtr0336703

NM_001275312

3073

778

CG12582-RH

FBtr0336704

NM_001275313

3029

906

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG12582-PA

FBpp0078603

103.5

908

6.02

NP_649436

AAF52172

CG12582-PD

FBpp0297164

103.5

908

6.02

NP_001246899

AFH06218

CG12582-PF

FBpp0307683

89.1

778

5.95

NP_730783

AAF52171

CG12582-PG

FBpp0307684

89.1

778

5.95

NP_001262241

AGB95624

CG12582-PH

FBpp0307685

103.2

906

6.09

NP_001262242

AGB95625

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Galactose-binding domain-like (IPR008979)

Glycoside hydrolase, catalytic domain (IPR013781)

Glycoside hydrolase, superfamily (IPR017853)

Glycoside hydrolase, family 2/20, immunoglobulin-like beta-sandwich domain (IPR013812)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval salivary gland body

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult midgut, adult Malpighian tubules, adult salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		109.35
Larval Midgut		412.4
Larval Hindgut		166.6
Larval Malpighian Tubules		440.9
Larval Fat Body		267.5
Larval Salivary Gland		128.1
Larval Trachea		196.975
Larval Carcass		132.95
Adult Head		231.3
Adult Eye		227.775
Adult Brain		109.8
Adult Thoracic-Abdominal Ganglion		122.4
Adult Crop		124.4
Adult Midgut		771.1
Adult Hindgut		214.2
Adult Malpighian Tubules		537.9
Adult Fat Body		268.8
Adult Salivary Gland		570.1
Adult Heart		251.85
Adult VirginFemale Spermatheca		419.8
Adult InseminatedFemale Spermatheca		356
Adult Ovary		136.6
Adult Testis		30
Adult Male Accessory Gland		243.7
Adult Carcass		219.2
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		109.35
Larval Midgut		412.4
Larval Hindgut		166.6
Larval Malpighian Tubules		440.9
Larval Fat Body		267.5
Larval Salivary Gland		128.1
Larval Trachea		196.975
Larval Carcass		132.95
Adult Head		231.3
Adult Eye		227.775
Adult Brain		109.8
Adult Thoracic-Abdominal Ganglion		122.4
Adult Crop		124.4
Adult Midgut		(771.1)
Adult Hindgut		214.2
Adult Malpighian Tubules		537.9
Adult Fat Body		268.8
Adult Salivary Gland		(570.1)
Adult Heart		251.85
Adult VirginFemale Spermatheca		419.8
Adult InseminatedFemale Spermatheca		356
Adult Ovary		136.6
Adult Testis		30
Adult Male Accessory Gland		243.7
Adult Carcass		219.2
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		109.35
Larval Midgut		412.4
Larval Hindgut		166.6
Larval Malpighian Tubules		440.9
Larval Fat Body		267.5
Larval Salivary Gland		128.1
Larval Trachea		196.975
Larval Carcass		132.95
Adult Head		231.3
Adult Eye		227.775
Adult Brain		109.8
Adult Thoracic-Abdominal Ganglion		122.4
Adult Crop		124.4
Adult Midgut		771.1
Adult Hindgut		214.2
Adult Malpighian Tubules		537.9
Adult Fat Body		268.8
Adult Salivary Gland		570.1
Adult Heart		251.85
Adult VirginFemale Spermatheca		419.8
Adult InseminatedFemale Spermatheca		356
Adult Ovary		136.6
Adult Testis		30
Adult Male Accessory Gland		243.7
Adult Carcass		219.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		109.35
Larval Midgut		412.4
Larval Hindgut		166.6
Larval Malpighian Tubules		440.9
Larval Fat Body		267.5
Larval Salivary Gland		128.1
Larval Trachea		196.975
Larval Carcass		132.95
Adult Head		231.3
Adult Eye		227.775
Adult Brain		109.8
Adult Thoracic-Abdominal Ganglion		122.4
Adult Crop		124.4
Adult Midgut		771.1
Adult Hindgut		214.2
Adult Malpighian Tubules		537.9
Adult Fat Body		268.8
Adult Salivary Gland		570.1
Adult Heart		251.85
Adult VirginFemale Spermatheca		419.8
Adult InseminatedFemale Spermatheca		356
Adult Ovary		136.6
Adult Testis		30
Adult Male Accessory Gland		243.7
Adult Carcass		219.2
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		109.35
Larval Midgut		412.4
Larval Hindgut		166.6
Larval Malpighian Tubules		440.9
Larval Fat Body		267.5
Larval Salivary Gland		128.1
Larval Trachea		196.975
Larval Carcass		132.95
Adult Head		231.3
Adult Eye		227.775
Adult Brain		109.8
Adult Thoracic-Abdominal Ganglion		122.4
Adult Crop		124.4
Adult Midgut		771.1
Adult Hindgut		214.2
Adult Malpighian Tubules		537.9
Adult Fat Body		268.8
Adult Salivary Gland		570.1
Adult Heart		251.85
Adult VirginFemale Spermatheca		419.8
Adult InseminatedFemale Spermatheca		356
Adult Ovary		136.6
Adult Testis		30
Adult Male Accessory Gland		243.7
Adult Carcass		219.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		109.35
Larval Midgut		412.4
Larval Hindgut		166.6
Larval Malpighian Tubules		440.9
Larval Fat Body		267.5
Larval Salivary Gland		128.1
Larval Trachea		196.975
Larval Carcass		132.95
Adult Head		231.3
Adult Eye		227.775
Adult Brain		109.8
Adult Thoracic-Abdominal Ganglion		122.4
Adult Crop		124.4
Adult Midgut		771.1
Adult Hindgut		214.2
Adult Malpighian Tubules		537.9
Adult Fat Body		268.8
Adult Salivary Gland		570.1
Adult Heart		251.85
Adult VirginFemale Spermatheca		419.8
Adult InseminatedFemale Spermatheca		356
Adult Ovary		136.6
Adult Testis		30
Adult Male Accessory Gland		243.7
Adult Carcass		219.2
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		109.35
Larval Midgut		412.4
Larval Hindgut		166.6
Larval Malpighian Tubules		440.9
Larval Fat Body		267.5
Larval Salivary Gland		128.1
Larval Trachea		196.975
Larval Carcass		132.95
Adult Head		231.3
Adult Eye		227.775
Adult Brain		109.8
Adult Thoracic-Abdominal Ganglion		122.4
Adult Crop		124.4
Adult Midgut		771.1
Adult Hindgut		214.2
Adult Malpighian Tubules		537.9
Adult Fat Body		268.8
Adult Salivary Gland		570.1
Adult Heart		251.85
Adult VirginFemale Spermatheca		419.8
Adult InseminatedFemale Spermatheca		356
Adult Ovary		136.6
Adult Testis		30
Adult Male Accessory Gland		243.7
Adult Carcass		219.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		109.35
Larval Midgut		412.4
Larval Hindgut		166.6
Larval Malpighian Tubules		440.9
Larval Fat Body		267.5
Larval Salivary Gland		128.1
Larval Trachea		196.975
Larval Carcass		132.95
Adult Head		231.3
Adult Eye		227.775
Adult Brain		109.8
Adult Thoracic-Abdominal Ganglion		122.4
Adult Crop		124.4
Adult Midgut		771.1
Adult Hindgut		214.2
Adult Malpighian Tubules		537.9
Adult Fat Body		268.8
Adult Salivary Gland		570.1
Adult Heart		251.85
Adult VirginFemale Spermatheca		419.8
Adult InseminatedFemale Spermatheca		356
Adult Ovary		136.6
Adult Testis		30
Adult Male Accessory Gland		243.7
Adult Carcass		219.2
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	231.3
NA	Eye	227.775
NA	Brain	109.8
109.35	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	122.4
NA	Crop	124.4
412.4	Midgut	771.1
166.6	Hindgut	214.2
440.9	Malpighian Tubules	537.9
267.5	Fat Body	268.8
128.1	Salivary Gland	570.1
NA	Heart	251.85
196.975	Trachea	NA
NA	VirginFemale Spermatheca	419.8
NA	InseminatedFemale Spermatheca	356
NA	Ovary	136.6
NA	Testis	30
NA	Male Accessory Gland	243.7
132.95	Carcass	219.2

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		8
central nervous system, pupae P8		7
head, virgin 1-day female		11
head, virgin 4-day female		12
head, virgin 20-day female		25
head, mated 1-day female		10
head, mated 4-day female		13
head, mated 20-day female		18
head, mated 1-day male		13
head, mated 4-day male		14
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		30
digestive system, 1-day adult		37
digestive system, 4-day adult		29
digestive system, 20-day adult		35
fat body, larvae L3 wandering		10
fat body, white prepupae		10
fat body, pupae P8		19
carcass, larvae L3 wandering		14
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		14
ovary, virgin 4-day female		22
ovary, mated 4-day female		17
testis, mated 4-day male		3
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		8
central nervous system, pupae P8		7
head, virgin 1-day female		11
head, virgin 4-day female		12
head, virgin 20-day female		25
head, mated 1-day female		10
head, mated 4-day female		13
head, mated 20-day female		18
head, mated 1-day male		13
head, mated 4-day male		14
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		(30)
digestive system, 1-day adult		(37)
digestive system, 4-day adult		(29)
digestive system, 20-day adult		(35)
fat body, larvae L3 wandering		10
fat body, white prepupae		10
fat body, pupae P8		19
carcass, larvae L3 wandering		14
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		14
ovary, virgin 4-day female		22
ovary, mated 4-day female		17
testis, mated 4-day male		3
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		8
central nervous system, pupae P8		7
head, virgin 1-day female		11
head, virgin 4-day female		12
head, virgin 20-day female		25
head, mated 1-day female		10
head, mated 4-day female		13
head, mated 20-day female		18
head, mated 1-day male		13
head, mated 4-day male		14
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		30
digestive system, 1-day adult		37
digestive system, 4-day adult		29
digestive system, 20-day adult		35
fat body, larvae L3 wandering		10
fat body, white prepupae		10
fat body, pupae P8		19
carcass, larvae L3 wandering		14
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		14
ovary, virgin 4-day female		22
ovary, mated 4-day female		17
testis, mated 4-day male		3
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		8
central nervous system, pupae P8		7
head, virgin 1-day female		11
head, virgin 4-day female		12
head, virgin 20-day female		25
head, mated 1-day female		10
head, mated 4-day female		13
head, mated 20-day female		18
head, mated 1-day male		13
head, mated 4-day male		14
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		30
digestive system, 1-day adult		37
digestive system, 4-day adult		29
digestive system, 20-day adult		35
fat body, larvae L3 wandering		10
fat body, white prepupae		10
fat body, pupae P8		19
carcass, larvae L3 wandering		14
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		14
ovary, virgin 4-day female		22
ovary, mated 4-day female		17
testis, mated 4-day male		3
accessory gland, mated 4-day male		7
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		8
central nervous system, pupae P8		7
head, virgin 1-day female		11
head, virgin 4-day female		12
head, virgin 20-day female		25
head, mated 1-day female		10
head, mated 4-day female		13
head, mated 20-day female		18
head, mated 1-day male		13
head, mated 4-day male		14
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		30
digestive system, 1-day adult		37
digestive system, 4-day adult		29
digestive system, 20-day adult		35
fat body, larvae L3 wandering		10
fat body, white prepupae		10
fat body, pupae P8		19
carcass, larvae L3 wandering		14
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		14
ovary, virgin 4-day female		22
ovary, mated 4-day female		17
testis, mated 4-day male		3
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		8
central nervous system, pupae P8		7
head, virgin 1-day female		11
head, virgin 4-day female		12
head, virgin 20-day female		25
head, mated 1-day female		10
head, mated 4-day female		13
head, mated 20-day female		18
head, mated 1-day male		13
head, mated 4-day male		14
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		30
digestive system, 1-day adult		(37)
digestive system, 4-day adult		29
digestive system, 20-day adult		(35)
fat body, larvae L3 wandering		10
fat body, white prepupae		10
fat body, pupae P8		19
carcass, larvae L3 wandering		14
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		14
ovary, virgin 4-day female		22
ovary, mated 4-day female		17
testis, mated 4-day male		3
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		8
central nervous system, pupae P8		7
head, virgin 1-day female		11
head, virgin 4-day female		12
head, virgin 20-day female		25
head, mated 1-day female		10
head, mated 4-day female		13
head, mated 20-day female		18
head, mated 1-day male		13
head, mated 4-day male		14
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		30
digestive system, 1-day adult		37
digestive system, 4-day adult		29
digestive system, 20-day adult		35
fat body, larvae L3 wandering		10
fat body, white prepupae		10
fat body, pupae P8		19
carcass, larvae L3 wandering		14
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		14
ovary, virgin 4-day female		22
ovary, mated 4-day female		17
testis, mated 4-day male		3
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		18
central nervous system, larvae L3		8
central nervous system, pupae P8		7
head, virgin 1-day female		11
head, virgin 4-day female		12
head, virgin 20-day female		25
head, mated 1-day female		10
head, mated 4-day female		13
head, mated 20-day female		18
head, mated 1-day male		13
head, mated 4-day male		14
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		30
digestive system, 1-day adult		37
digestive system, 4-day adult		29
digestive system, 20-day adult		35
fat body, larvae L3 wandering		10
fat body, white prepupae		10
fat body, pupae P8		19
carcass, larvae L3 wandering		14
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		14
ovary, virgin 4-day female		22
ovary, mated 4-day female		17
testis, mated 4-day male		3
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		27
embryo 02-04hr		11
embryo 04-06hr		8
embryo 06-08hr		8
embryo 08-10hr		8
embryo 10-12hr		12
embryo 12-14hr		17
embryo 14-16hr		14
embryo 16-18hr		14
embryo 18-20hr		12
embryo 20-22hr		14
embryo 22-24hr		13
larva L1		18
larva L2		17
larva L3 12hr old		16
larva L3 puffstage 1-2		28
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		34
white prepupae new		31
white prepupae 12hr		31
white prepupae 24hr		42
pupae 2d postWPP		33
pupae 3d postWPP		13
pupae 4d postWPP		13
adult male 01day		23
adult male 05day		27
adult male 30day		26
adult female 01day		26
adult female 05day		34
adult female 30day		33
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(27)
embryo 02-04hr		11
embryo 04-06hr		8
embryo 06-08hr		8
embryo 08-10hr		8
embryo 10-12hr		12
embryo 12-14hr		17
embryo 14-16hr		14
embryo 16-18hr		14
embryo 18-20hr		12
embryo 20-22hr		14
embryo 22-24hr		13
larva L1		18
larva L2		17
larva L3 12hr old		16
larva L3 puffstage 1-2		(28)
larva L3 puffstage 3-6		(27)
larva L3 puffstage 7-9		(34)
white prepupae new		(31)
white prepupae 12hr		(31)
white prepupae 24hr		(42)
pupae 2d postWPP		(33)
pupae 3d postWPP		13
pupae 4d postWPP		13
adult male 01day		23
adult male 05day		(27)
adult male 30day		26
adult female 01day		26
adult female 05day		(34)
adult female 30day		(33)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		27
embryo 02-04hr		11
embryo 04-06hr		8
embryo 06-08hr		8
embryo 08-10hr		8
embryo 10-12hr		12
embryo 12-14hr		17
embryo 14-16hr		14
embryo 16-18hr		14
embryo 18-20hr		12
embryo 20-22hr		14
embryo 22-24hr		13
larva L1		18
larva L2		17
larva L3 12hr old		16
larva L3 puffstage 1-2		28
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		34
white prepupae new		31
white prepupae 12hr		31
white prepupae 24hr		42
pupae 2d postWPP		33
pupae 3d postWPP		13
pupae 4d postWPP		13
adult male 01day		23
adult male 05day		27
adult male 30day		26
adult female 01day		26
adult female 05day		34
adult female 30day		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		27
embryo 02-04hr		11
embryo 04-06hr		8
embryo 06-08hr		8
embryo 08-10hr		8
embryo 10-12hr		12
embryo 12-14hr		17
embryo 14-16hr		14
embryo 16-18hr		14
embryo 18-20hr		12
embryo 20-22hr		14
embryo 22-24hr		13
larva L1		18
larva L2		17
larva L3 12hr old		16
larva L3 puffstage 1-2		28
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		34
white prepupae new		31
white prepupae 12hr		31
white prepupae 24hr		42
pupae 2d postWPP		33
pupae 3d postWPP		13
pupae 4d postWPP		13
adult male 01day		23
adult male 05day		27
adult male 30day		26
adult female 01day		26
adult female 05day		34
adult female 30day		33
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		27
embryo 02-04hr		11
embryo 04-06hr		8
embryo 06-08hr		8
embryo 08-10hr		8
embryo 10-12hr		12
embryo 12-14hr		17
embryo 14-16hr		14
embryo 16-18hr		14
embryo 18-20hr		12
embryo 20-22hr		14
embryo 22-24hr		13
larva L1		18
larva L2		17
larva L3 12hr old		16
larva L3 puffstage 1-2		28
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		34
white prepupae new		31
white prepupae 12hr		31
white prepupae 24hr		42
pupae 2d postWPP		33
pupae 3d postWPP		13
pupae 4d postWPP		13
adult male 01day		23
adult male 05day		27
adult male 30day		26
adult female 01day		26
adult female 05day		34
adult female 30day		33
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		27
embryo 02-04hr		11
embryo 04-06hr		8
embryo 06-08hr		8
embryo 08-10hr		8
embryo 10-12hr		12
embryo 12-14hr		17
embryo 14-16hr		14
embryo 16-18hr		14
embryo 18-20hr		12
embryo 20-22hr		14
embryo 22-24hr		13
larva L1		18
larva L2		17
larva L3 12hr old		16
larva L3 puffstage 1-2		28
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		(34)
white prepupae new		31
white prepupae 12hr		31
white prepupae 24hr		(42)
pupae 2d postWPP		(33)
pupae 3d postWPP		13
pupae 4d postWPP		13
adult male 01day		23
adult male 05day		27
adult male 30day		26
adult female 01day		26
adult female 05day		(34)
adult female 30day		(33)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		27
embryo 02-04hr		11
embryo 04-06hr		8
embryo 06-08hr		8
embryo 08-10hr		8
embryo 10-12hr		12
embryo 12-14hr		17
embryo 14-16hr		14
embryo 16-18hr		14
embryo 18-20hr		12
embryo 20-22hr		14
embryo 22-24hr		13
larva L1		18
larva L2		17
larva L3 12hr old		16
larva L3 puffstage 1-2		28
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		34
white prepupae new		31
white prepupae 12hr		31
white prepupae 24hr		42
pupae 2d postWPP		33
pupae 3d postWPP		13
pupae 4d postWPP		13
adult male 01day		23
adult male 05day		27
adult male 30day		26
adult female 01day		26
adult female 05day		34
adult female 30day		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		27
embryo 02-04hr		11
embryo 04-06hr		8
embryo 06-08hr		8
embryo 08-10hr		8
embryo 10-12hr		12
embryo 12-14hr		17
embryo 14-16hr		14
embryo 16-18hr		14
embryo 18-20hr		12
embryo 20-22hr		14
embryo 22-24hr		13
larva L1		18
larva L2		17
larva L3 12hr old		16
larva L3 puffstage 1-2		28
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		34
white prepupae new		31
white prepupae 12hr		31
white prepupae 24hr		42
pupae 2d postWPP		33
pupae 3d postWPP		13
pupae 4d postWPP		13
adult male 01day		23
adult male 05day		27
adult male 30day		26
adult female 01day		26
adult female 05day		34
adult female 30day		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		0
embryonic 1182-4H		48
embryonic GM2		46
embryonic Kc167		33
embryonic S1		53
embryonic S3		25
leg disc CME L1		0
wing disc CME-W2		2
wing disc ML-DmD8		45
wing disc ML-DmD9		47
wing disc ML-DmD16-c3		16
wing disc ML-DmD21		1
wing disc ML-DmD32		49
haltere disc ML-DmD17-c3		13
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		2
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		39
CNS ML-DmBG2-c2		44
tumorous blood cells mbn2		75
ovary fGS/OSS		6
ovary OSC		26
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(78)
Schneider line 2 Sg4		0
embryonic 1182-4H		(48)
embryonic GM2		(46)
embryonic Kc167		(33)
embryonic S1		(53)
embryonic S3		25
leg disc CME L1		0
wing disc CME-W2		2
wing disc ML-DmD8		(45)
wing disc ML-DmD9		(47)
wing disc ML-DmD16-c3		16
wing disc ML-DmD21		1
wing disc ML-DmD32		(49)
haltere disc ML-DmD17-c3		13
eye-antennal disc ML-DmD11		(54)
antennal disc ML-DmD20-c5		2
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		(39)
CNS ML-DmBG2-c2		(44)
tumorous blood cells mbn2		(75)
ovary fGS/OSS		6
ovary OSC		26
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		0
embryonic 1182-4H		48
embryonic GM2		46
embryonic Kc167		33
embryonic S1		53
embryonic S3		25
leg disc CME L1		0
wing disc CME-W2		2
wing disc ML-DmD8		45
wing disc ML-DmD9		47
wing disc ML-DmD16-c3		16
wing disc ML-DmD21		1
wing disc ML-DmD32		49
haltere disc ML-DmD17-c3		13
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		2
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		39
CNS ML-DmBG2-c2		44
tumorous blood cells mbn2		75
ovary fGS/OSS		6
ovary OSC		26
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		0
embryonic 1182-4H		48
embryonic GM2		46
embryonic Kc167		33
embryonic S1		53
embryonic S3		25
leg disc CME L1		0
wing disc CME-W2		2
wing disc ML-DmD8		45
wing disc ML-DmD9		47
wing disc ML-DmD16-c3		16
wing disc ML-DmD21		1
wing disc ML-DmD32		49
haltere disc ML-DmD17-c3		13
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		2
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		39
CNS ML-DmBG2-c2		44
tumorous blood cells mbn2		75
ovary fGS/OSS		6
ovary OSC		26
ovary OSS		12
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		0
embryonic 1182-4H		48
embryonic GM2		46
embryonic Kc167		33
embryonic S1		53
embryonic S3		25
leg disc CME L1		0
wing disc CME-W2		2
wing disc ML-DmD8		45
wing disc ML-DmD9		47
wing disc ML-DmD16-c3		16
wing disc ML-DmD21		1
wing disc ML-DmD32		49
haltere disc ML-DmD17-c3		13
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		2
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		39
CNS ML-DmBG2-c2		44
tumorous blood cells mbn2		75
ovary fGS/OSS		6
ovary OSC		26
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(78)
Schneider line 2 Sg4		0
embryonic 1182-4H		(48)
embryonic GM2		(46)
embryonic Kc167		(33)
embryonic S1		(53)
embryonic S3		25
leg disc CME L1		0
wing disc CME-W2		2
wing disc ML-DmD8		(45)
wing disc ML-DmD9		(47)
wing disc ML-DmD16-c3		16
wing disc ML-DmD21		1
wing disc ML-DmD32		(49)
haltere disc ML-DmD17-c3		13
eye-antennal disc ML-DmD11		(54)
antennal disc ML-DmD20-c5		2
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		(39)
CNS ML-DmBG2-c2		(44)
tumorous blood cells mbn2		(75)
ovary fGS/OSS		6
ovary OSC		26
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		0
embryonic 1182-4H		48
embryonic GM2		46
embryonic Kc167		33
embryonic S1		53
embryonic S3		25
leg disc CME L1		0
wing disc CME-W2		2
wing disc ML-DmD8		45
wing disc ML-DmD9		47
wing disc ML-DmD16-c3		16
wing disc ML-DmD21		1
wing disc ML-DmD32		49
haltere disc ML-DmD17-c3		13
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		2
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		39
CNS ML-DmBG2-c2		44
tumorous blood cells mbn2		75
ovary fGS/OSS		6
ovary OSC		26
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		0
embryonic 1182-4H		48
embryonic GM2		46
embryonic Kc167		33
embryonic S1		53
embryonic S3		25
leg disc CME L1		0
wing disc CME-W2		2
wing disc ML-DmD8		45
wing disc ML-DmD9		47
wing disc ML-DmD16-c3		16
wing disc ML-DmD21		1
wing disc ML-DmD32		49
haltere disc ML-DmD17-c3		13
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		2
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		39
CNS ML-DmBG2-c2		44
tumorous blood cells mbn2		75
ovary fGS/OSS		6
ovary OSC		26
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		15
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		8
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		14
Caffeine 1.5 mg/ml 4 hrs, larvae L3		18
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		16
Caffeine 25 mg/ml 48 hrs, 4-day adult		7
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		21
Rotenone 2 μg 12 hrs, larvae L3		21
Rotenone 8 μg 12 hrs, larvae L3		16
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		15
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		8
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		14
Caffeine 1.5 mg/ml 4 hrs, larvae L3		18
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		16
Caffeine 25 mg/ml 48 hrs, 4-day adult		7
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		21
Rotenone 2 μg 12 hrs, larvae L3		21
Rotenone 8 μg 12 hrs, larvae L3		16
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		15
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		8
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		14
Caffeine 1.5 mg/ml 4 hrs, larvae L3		18
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		16
Caffeine 25 mg/ml 48 hrs, 4-day adult		7
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		21
Rotenone 2 μg 12 hrs, larvae L3		21
Rotenone 8 μg 12 hrs, larvae L3		16
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		15
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		8
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		14
Caffeine 1.5 mg/ml 4 hrs, larvae L3		18
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		16
Caffeine 25 mg/ml 48 hrs, 4-day adult		7
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		21
Rotenone 2 μg 12 hrs, larvae L3		21
Rotenone 8 μg 12 hrs, larvae L3		16
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		15
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		8
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		14
Caffeine 1.5 mg/ml 4 hrs, larvae L3		18
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		16
Caffeine 25 mg/ml 48 hrs, 4-day adult		7
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		21
Rotenone 2 μg 12 hrs, larvae L3		21
Rotenone 8 μg 12 hrs, larvae L3		16
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		15
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		8
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		14
Caffeine 1.5 mg/ml 4 hrs, larvae L3		18
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		16
Caffeine 25 mg/ml 48 hrs, 4-day adult		7
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		21
Rotenone 2 μg 12 hrs, larvae L3		21
Rotenone 8 μg 12 hrs, larvae L3		16
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		15
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		8
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		14
Caffeine 1.5 mg/ml 4 hrs, larvae L3		18
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		16
Caffeine 25 mg/ml 48 hrs, 4-day adult		7
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		21
Rotenone 2 μg 12 hrs, larvae L3		21
Rotenone 8 μg 12 hrs, larvae L3		16
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		19
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		15
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		8
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		14
Caffeine 1.5 mg/ml 4 hrs, larvae L3		18
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		16
Caffeine 25 mg/ml 48 hrs, 4-day adult		7
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		21
Rotenone 2 μg 12 hrs, larvae L3		21
Rotenone 8 μg 12 hrs, larvae L3		16
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

GH09594

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0037215

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG12582-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for CG12582

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

CG12582^GD4924

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of CG12582	Class	Mutagen	Stocks	Known lesion
CG12582^c02954		piggyBac transposase	1	--
CG12582^MI03739			1	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG12582	Class	Mutagen	Stocks	Known lesion
CG12582^GD4924		in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG12582^KK101964		in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4924}

P{KK101964}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

Mi{MIC}CG12582^MI03739

PBac{PB}CG12582^c02954

Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 2 terms )

Molecular Function

CV term

Evidence

References

beta-mannosidase activity

inferred from sequence or structural similarity with UniProtKB:Q95327

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

carbohydrate metabolic process

inferred from electronic annotation with InterPro:IPR013781, InterPro:IPR013812

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

CG12582 - CG11911

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

CG12582 - CG31199

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

CG12582 - Jon65Ai

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

CG12582 - P58IPK

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG12582 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

65747

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0037215

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

40524

Orthologs

OrthoDB Orthologs (42) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6JT0QT

Dipteran inclusive ortholog search

EOG6HDT2J

Insect inclusive ortholog search

EOG612K18

Arthropod inclusive ortholog search

EOG66HDRH

Metazoa inclusive ortholog search

EOG690M2N

Orthologs in Drosophila Species (EOG6JT0QT)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11702

Drosophila persimilis

Dper\GL12304

Drosophila willistoni

Dwil\GK12069

Drosophila virilis

Dvir\GJ14154

Drosophila mojavensis

Dmoj\GI22853

Drosophila grimshawi

Dgri\GH18706

Orthologs in non-Drosophila Dipterans (EOG6HDT2J)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013867

Anopheles gambiae

Malaria mosquito

Agam\AGAP000143

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ012655

Orthologs in non-Dipteran Insects (EOG612K18)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB42077

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011021

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH27234

Atta cephalotes

Leafcutter ant

Acep\ACEP10594

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO16733

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL19381

Linepithema humile

Argentine ant

Lhum\LH20716

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB22987

Solenopsis invicta

Red fire ant

Sinv\SINV20158

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001760

Bombyx mori

Silkmoth

Bmor\BGIBMGA004998

Pediculus humanus

Human body louse

Phum\PHUM193470

Tribolium castaneum

Red flour beetle

Tcas\TC009883

Tribolium castaneum

Red flour beetle

Tcas\TC010171

Orthologs in non-Insect Arthropods (EOG66HDRH)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\PB1-F2

Daphnia pulex

Water flea

Dpux\Sox4

Ixodes scapularis

Deer tick

Isca\ISCW005511

Orthologs in non-Arthropod Metazoa (EOG690M2N)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\C33G3.4

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_011215gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008458gn

Danio rerio

Zebrafish

Drer\CU929290.1

Danio rerio

Zebrafish

Drer\ENSDARG00000008238

Xenopus tropicalis

Western clawed frog

Xtro\manba

Gallus gallus

Domestic chicken

Ggal\MANBA

Mus musculus

House mouse

Mmus\ENSMUSG00000028164

Rattus norvegicus

Norway rat

Rnor\Manba

Homo sapiens

Human

Hsap\MANBA

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\MANBA

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11702

Drosophila persimilis

Dper\GL12304

Drosophila willistoni

Dwil\GK12069

Drosophila virilis

Dvir\GJ14154

Drosophila grimshawi

Dgri\GH18706

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Bloomington

37081

y¹ w^*; Mi{MIC}CG12582^MI03739

Harvard

PBac{PB}CG12582^c02954

VDRC

v15028

w¹¹¹⁸; P{GD4924}v15028

Genomic Clones ( 1 )

BACR15O02

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 63 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

FI03673

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0037215

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

40524

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG12582 CG12583

(FlyBase, 1996-)

Additional comments

Source for merge of CG12582 CG12583 was a shared cDNA (date:010720).

(FlyBase, 1996-)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

40524

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Galactose-binding domain-like (IPR008979)

Glycoside hydrolase, catalytic domain (IPR013781)

Glycoside hydrolase, superfamily (IPR017853)

Glycoside hydrolase, family 2/20, immunoglobulin-like beta-sandwich domain (IPR013812)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

GH09594

Linkouts

BioGRID - A database of protein and genetic interactions

•

65747

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0037215

DRSC - Results from RNAi screens.

•

FBgn0037215

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0037215

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG12582-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0037215

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

40524

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

40524

modMine - Data generated by the modENCODE project.

•

FBgn0037215

Synonyms & Secondary IDs ( 2 )

Reported As

Symbol Synonym

CG12582

(Keleman et al., 2009.8.5)

CG12583

Name Synonym

Secondary FlyBase IDs

FBan0012582
FBgn0037216

References ( 20 )

Research paper

Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784: GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834]
Inlow and Restifo, 2004, Genetics 166(2): 835--881: Molecular and comparative genetics of mental retardation. [FBrf0174714]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
Guruharsha et al., 2011, Cell 147(3):: Table S3. [FBrf0218395]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Bellen, 2011.10.25, set956.xml: set956.xml [FBrf0216550]
Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project: DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Drosophila Heterochromatin Genome Project, 2006, Release 5.1 of the annotated Drosophila melanogaster heterochromatin.: Release 5.1 of the annotated Drosophila melanogaster heterochromatin. [FBrf0188763]
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file): Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Gene Disruption Project members, 2001-, (Computer file): (Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation.: FlyBase curation. [FBrf0105495]

Website

Fisher et al., 2012, BDGP insitu homepage: BDGP insitu homepage [FBrf0219073]

Curated genome annotation

Evangelista, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126663]