Gene Dmel\CG12173

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General Information

Symbol

Dmel\CG12173

Species

D. melanogaster

Name

Annotation symbol

CG12173

Feature type

protein_coding_gene

FlyBase ID

FBgn0037305

Gene Model Status

Current

Stock availability

3 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

82F7-82F7

Sequence location

3R:1,081,166..1,082,403 [-]

Genomic Maps

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Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG12173 (FBgn0037305). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: acireductone synthase activity; magnesium ion binding; phosphoglycolate phosphatase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: L-methionine salvage from methylthioadenosine. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Enolase-phosphatase E1; HAD-like domain; HAD-superfamily hydrolase, subfamily IA, variant 1; Haloacid dehalogenase/epoxide hydrolase. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 and 18-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1630073_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of CG12173. Gene sequence location is 3R:1081166..1082403.

User Contributed Data

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Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

82F7-82F7

Limits computationally determined from genome sequence between P{PZ}l(3)02733⁰²⁷³³ and P{EP}EP974

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG12173 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG12173-RA

FBtr0078821

NM_141266

867

256

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG12173-PA

FBpp0078463

28.3

256

5.96

NP_649523

AAF52053

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Haloacid dehalogenase/epoxide hydrolase (IPR005833)

HAD-superfamily hydrolase, subfamily IA, variant 1 (IPR006439)

Enolase-phosphatase E1 (IPR023943)

Enolase-phosphatase E1, eukaryotes (IPR027511)

HAD-like domain (IPR023214)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VN95

UniProtKB/TrEMBL

E1JJ50

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		340.425
Larval Midgut		182.7
Larval Hindgut		243.2
Larval Malpighian Tubules		414.2
Larval Fat Body		184.9
Larval Salivary Gland		156.2
Larval Trachea		345.375
Larval Carcass		160.025
Adult Head		187.1
Adult Eye		211.425
Adult Brain		217.4
Adult Thoracic-Abdominal Ganglion		189.9
Adult Crop		180.3
Adult Midgut		238.4
Adult Hindgut		338.3
Adult Malpighian Tubules		551.7
Adult Fat Body		185.1
Adult Salivary Gland		132.2
Adult Heart		199.375
Adult VirginFemale Spermatheca		198.2
Adult InseminatedFemale Spermatheca		186.7
Adult Ovary		350.9
Adult Testis		106.8
Adult Male Accessory Gland		176
Adult Carcass		144.3
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		340.425
Larval Midgut		182.7
Larval Hindgut		243.2
Larval Malpighian Tubules		414.2
Larval Fat Body		184.9
Larval Salivary Gland		156.2
Larval Trachea		345.375
Larval Carcass		160.025
Adult Head		187.1
Adult Eye		211.425
Adult Brain		217.4
Adult Thoracic-Abdominal Ganglion		189.9
Adult Crop		180.3
Adult Midgut		238.4
Adult Hindgut		338.3
Adult Malpighian Tubules		(551.7)
Adult Fat Body		185.1
Adult Salivary Gland		132.2
Adult Heart		199.375
Adult VirginFemale Spermatheca		198.2
Adult InseminatedFemale Spermatheca		186.7
Adult Ovary		350.9
Adult Testis		106.8
Adult Male Accessory Gland		176
Adult Carcass		144.3
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		340.425
Larval Midgut		182.7
Larval Hindgut		243.2
Larval Malpighian Tubules		414.2
Larval Fat Body		184.9
Larval Salivary Gland		156.2
Larval Trachea		345.375
Larval Carcass		160.025
Adult Head		187.1
Adult Eye		211.425
Adult Brain		217.4
Adult Thoracic-Abdominal Ganglion		189.9
Adult Crop		180.3
Adult Midgut		238.4
Adult Hindgut		338.3
Adult Malpighian Tubules		551.7
Adult Fat Body		185.1
Adult Salivary Gland		132.2
Adult Heart		199.375
Adult VirginFemale Spermatheca		198.2
Adult InseminatedFemale Spermatheca		186.7
Adult Ovary		350.9
Adult Testis		106.8
Adult Male Accessory Gland		176
Adult Carcass		144.3
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		340.425
Larval Midgut		182.7
Larval Hindgut		243.2
Larval Malpighian Tubules		414.2
Larval Fat Body		184.9
Larval Salivary Gland		156.2
Larval Trachea		345.375
Larval Carcass		160.025
Adult Head		187.1
Adult Eye		211.425
Adult Brain		217.4
Adult Thoracic-Abdominal Ganglion		189.9
Adult Crop		180.3
Adult Midgut		238.4
Adult Hindgut		338.3
Adult Malpighian Tubules		551.7
Adult Fat Body		185.1
Adult Salivary Gland		132.2
Adult Heart		199.375
Adult VirginFemale Spermatheca		198.2
Adult InseminatedFemale Spermatheca		186.7
Adult Ovary		350.9
Adult Testis		106.8
Adult Male Accessory Gland		176
Adult Carcass		144.3
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		340.425
Larval Midgut		182.7
Larval Hindgut		243.2
Larval Malpighian Tubules		414.2
Larval Fat Body		184.9
Larval Salivary Gland		156.2
Larval Trachea		345.375
Larval Carcass		160.025
Adult Head		187.1
Adult Eye		211.425
Adult Brain		217.4
Adult Thoracic-Abdominal Ganglion		189.9
Adult Crop		180.3
Adult Midgut		238.4
Adult Hindgut		338.3
Adult Malpighian Tubules		551.7
Adult Fat Body		185.1
Adult Salivary Gland		132.2
Adult Heart		199.375
Adult VirginFemale Spermatheca		198.2
Adult InseminatedFemale Spermatheca		186.7
Adult Ovary		350.9
Adult Testis		106.8
Adult Male Accessory Gland		176
Adult Carcass		144.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		340.425
Larval Midgut		182.7
Larval Hindgut		243.2
Larval Malpighian Tubules		414.2
Larval Fat Body		184.9
Larval Salivary Gland		156.2
Larval Trachea		345.375
Larval Carcass		160.025
Adult Head		187.1
Adult Eye		211.425
Adult Brain		217.4
Adult Thoracic-Abdominal Ganglion		189.9
Adult Crop		180.3
Adult Midgut		238.4
Adult Hindgut		338.3
Adult Malpighian Tubules		551.7
Adult Fat Body		185.1
Adult Salivary Gland		132.2
Adult Heart		199.375
Adult VirginFemale Spermatheca		198.2
Adult InseminatedFemale Spermatheca		186.7
Adult Ovary		350.9
Adult Testis		106.8
Adult Male Accessory Gland		176
Adult Carcass		144.3
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		340.425
Larval Midgut		182.7
Larval Hindgut		243.2
Larval Malpighian Tubules		414.2
Larval Fat Body		184.9
Larval Salivary Gland		156.2
Larval Trachea		345.375
Larval Carcass		160.025
Adult Head		187.1
Adult Eye		211.425
Adult Brain		217.4
Adult Thoracic-Abdominal Ganglion		189.9
Adult Crop		180.3
Adult Midgut		238.4
Adult Hindgut		338.3
Adult Malpighian Tubules		551.7
Adult Fat Body		185.1
Adult Salivary Gland		132.2
Adult Heart		199.375
Adult VirginFemale Spermatheca		198.2
Adult InseminatedFemale Spermatheca		186.7
Adult Ovary		350.9
Adult Testis		106.8
Adult Male Accessory Gland		176
Adult Carcass		144.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		340.425
Larval Midgut		182.7
Larval Hindgut		243.2
Larval Malpighian Tubules		414.2
Larval Fat Body		184.9
Larval Salivary Gland		156.2
Larval Trachea		345.375
Larval Carcass		160.025
Adult Head		187.1
Adult Eye		211.425
Adult Brain		217.4
Adult Thoracic-Abdominal Ganglion		189.9
Adult Crop		180.3
Adult Midgut		238.4
Adult Hindgut		338.3
Adult Malpighian Tubules		551.7
Adult Fat Body		185.1
Adult Salivary Gland		132.2
Adult Heart		199.375
Adult VirginFemale Spermatheca		198.2
Adult InseminatedFemale Spermatheca		186.7
Adult Ovary		350.9
Adult Testis		106.8
Adult Male Accessory Gland		176
Adult Carcass		144.3
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	187.1
NA	Eye	211.425
NA	Brain	217.4
340.425	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	189.9
NA	Crop	180.3
182.7	Midgut	238.4
243.2	Hindgut	338.3
414.2	Malpighian Tubules	551.7
184.9	Fat Body	185.1
156.2	Salivary Gland	132.2
NA	Heart	199.375
345.375	Trachea	NA
NA	VirginFemale Spermatheca	198.2
NA	InseminatedFemale Spermatheca	186.7
NA	Ovary	350.9
NA	Testis	106.8
NA	Male Accessory Gland	176
160.025	Carcass	144.3

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		3
head, virgin 4-day female		3
head, virgin 20-day female		6
head, mated 1-day female		3
head, mated 4-day female		5
head, mated 20-day female		5
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		5
fat body, pupae P8		0
carcass, larvae L3 wandering		9
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		5
ovary, virgin 4-day female		15
ovary, mated 4-day female		9
testis, mated 4-day male		15
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		3
head, virgin 4-day female		3
head, virgin 20-day female		6
head, mated 1-day female		3
head, mated 4-day female		5
head, mated 20-day female		5
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		5
fat body, pupae P8		0
carcass, larvae L3 wandering		9
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		5
ovary, virgin 4-day female		15
ovary, mated 4-day female		9
testis, mated 4-day male		15
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		3
head, virgin 4-day female		3
head, virgin 20-day female		6
head, mated 1-day female		3
head, mated 4-day female		5
head, mated 20-day female		5
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		5
fat body, pupae P8		0
carcass, larvae L3 wandering		9
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		5
ovary, virgin 4-day female		15
ovary, mated 4-day female		9
testis, mated 4-day male		15
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		3
head, virgin 4-day female		3
head, virgin 20-day female		6
head, mated 1-day female		3
head, mated 4-day female		5
head, mated 20-day female		5
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		5
fat body, pupae P8		0
carcass, larvae L3 wandering		9
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		5
ovary, virgin 4-day female		15
ovary, mated 4-day female		9
testis, mated 4-day male		15
accessory gland, mated 4-day male		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		3
head, virgin 4-day female		3
head, virgin 20-day female		6
head, mated 1-day female		3
head, mated 4-day female		5
head, mated 20-day female		5
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		5
fat body, pupae P8		0
carcass, larvae L3 wandering		9
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		5
ovary, virgin 4-day female		15
ovary, mated 4-day female		9
testis, mated 4-day male		15
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		3
head, virgin 4-day female		3
head, virgin 20-day female		6
head, mated 1-day female		3
head, mated 4-day female		5
head, mated 20-day female		5
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		5
fat body, pupae P8		0
carcass, larvae L3 wandering		9
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		5
ovary, virgin 4-day female		15
ovary, mated 4-day female		9
testis, mated 4-day male		15
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		3
head, virgin 4-day female		3
head, virgin 20-day female		6
head, mated 1-day female		3
head, mated 4-day female		5
head, mated 20-day female		5
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		5
fat body, pupae P8		0
carcass, larvae L3 wandering		9
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		5
ovary, virgin 4-day female		15
ovary, mated 4-day female		9
testis, mated 4-day male		15
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		10
central nervous system, pupae P8		7
head, virgin 1-day female		3
head, virgin 4-day female		3
head, virgin 20-day female		6
head, mated 1-day female		3
head, mated 4-day female		5
head, mated 20-day female		5
head, mated 1-day male		4
head, mated 4-day male		6
head, mated 20-day male		6
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		5
fat body, pupae P8		0
carcass, larvae L3 wandering		9
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		5
ovary, virgin 4-day female		15
ovary, mated 4-day female		9
testis, mated 4-day male		15
accessory gland, mated 4-day male		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 and 18-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		31
embryo 02-04hr		5
embryo 04-06hr		5
embryo 06-08hr		6
embryo 08-10hr		5
embryo 10-12hr		9
embryo 12-14hr		9
embryo 14-16hr		9
embryo 16-18hr		11
embryo 18-20hr		12
embryo 20-22hr		16
embryo 22-24hr		19
larva L1		29
larva L2		31
larva L3 12hr old		25
larva L3 puffstage 1-2		26
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		22
white prepupae new		23
white prepupae 12hr		24
white prepupae 24hr		23
pupae 2d postWPP		19
pupae 3d postWPP		16
pupae 4d postWPP		13
adult male 01day		18
adult male 05day		18
adult male 30day		16
adult female 01day		25
adult female 05day		40
adult female 30day		38
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(31)
embryo 02-04hr		5
embryo 04-06hr		5
embryo 06-08hr		6
embryo 08-10hr		5
embryo 10-12hr		9
embryo 12-14hr		9
embryo 14-16hr		9
embryo 16-18hr		11
embryo 18-20hr		12
embryo 20-22hr		16
embryo 22-24hr		19
larva L1		(29)
larva L2		(31)
larva L3 12hr old		25
larva L3 puffstage 1-2		26
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		22
white prepupae new		23
white prepupae 12hr		24
white prepupae 24hr		23
pupae 2d postWPP		19
pupae 3d postWPP		16
pupae 4d postWPP		13
adult male 01day		18
adult male 05day		18
adult male 30day		16
adult female 01day		25
adult female 05day		(40)
adult female 30day		(38)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		31
embryo 02-04hr		5
embryo 04-06hr		5
embryo 06-08hr		6
embryo 08-10hr		5
embryo 10-12hr		9
embryo 12-14hr		9
embryo 14-16hr		9
embryo 16-18hr		11
embryo 18-20hr		12
embryo 20-22hr		16
embryo 22-24hr		19
larva L1		29
larva L2		31
larva L3 12hr old		25
larva L3 puffstage 1-2		26
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		22
white prepupae new		23
white prepupae 12hr		24
white prepupae 24hr		23
pupae 2d postWPP		19
pupae 3d postWPP		16
pupae 4d postWPP		13
adult male 01day		18
adult male 05day		18
adult male 30day		16
adult female 01day		25
adult female 05day		40
adult female 30day		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		31
embryo 02-04hr		5
embryo 04-06hr		5
embryo 06-08hr		6
embryo 08-10hr		5
embryo 10-12hr		9
embryo 12-14hr		9
embryo 14-16hr		9
embryo 16-18hr		11
embryo 18-20hr		12
embryo 20-22hr		16
embryo 22-24hr		19
larva L1		29
larva L2		31
larva L3 12hr old		25
larva L3 puffstage 1-2		26
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		22
white prepupae new		23
white prepupae 12hr		24
white prepupae 24hr		23
pupae 2d postWPP		19
pupae 3d postWPP		16
pupae 4d postWPP		13
adult male 01day		18
adult male 05day		18
adult male 30day		16
adult female 01day		25
adult female 05day		40
adult female 30day		38
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		31
embryo 02-04hr		5
embryo 04-06hr		5
embryo 06-08hr		6
embryo 08-10hr		5
embryo 10-12hr		9
embryo 12-14hr		9
embryo 14-16hr		9
embryo 16-18hr		11
embryo 18-20hr		12
embryo 20-22hr		16
embryo 22-24hr		19
larva L1		29
larva L2		31
larva L3 12hr old		25
larva L3 puffstage 1-2		26
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		22
white prepupae new		23
white prepupae 12hr		24
white prepupae 24hr		23
pupae 2d postWPP		19
pupae 3d postWPP		16
pupae 4d postWPP		13
adult male 01day		18
adult male 05day		18
adult male 30day		16
adult female 01day		25
adult female 05day		40
adult female 30day		38
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		31
embryo 02-04hr		5
embryo 04-06hr		5
embryo 06-08hr		6
embryo 08-10hr		5
embryo 10-12hr		9
embryo 12-14hr		9
embryo 14-16hr		9
embryo 16-18hr		11
embryo 18-20hr		12
embryo 20-22hr		16
embryo 22-24hr		19
larva L1		29
larva L2		31
larva L3 12hr old		25
larva L3 puffstage 1-2		26
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		22
white prepupae new		23
white prepupae 12hr		24
white prepupae 24hr		23
pupae 2d postWPP		19
pupae 3d postWPP		16
pupae 4d postWPP		13
adult male 01day		18
adult male 05day		18
adult male 30day		16
adult female 01day		25
adult female 05day		(40)
adult female 30day		(38)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		31
embryo 02-04hr		5
embryo 04-06hr		5
embryo 06-08hr		6
embryo 08-10hr		5
embryo 10-12hr		9
embryo 12-14hr		9
embryo 14-16hr		9
embryo 16-18hr		11
embryo 18-20hr		12
embryo 20-22hr		16
embryo 22-24hr		19
larva L1		29
larva L2		31
larva L3 12hr old		25
larva L3 puffstage 1-2		26
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		22
white prepupae new		23
white prepupae 12hr		24
white prepupae 24hr		23
pupae 2d postWPP		19
pupae 3d postWPP		16
pupae 4d postWPP		13
adult male 01day		18
adult male 05day		18
adult male 30day		16
adult female 01day		25
adult female 05day		40
adult female 30day		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		31
embryo 02-04hr		5
embryo 04-06hr		5
embryo 06-08hr		6
embryo 08-10hr		5
embryo 10-12hr		9
embryo 12-14hr		9
embryo 14-16hr		9
embryo 16-18hr		11
embryo 18-20hr		12
embryo 20-22hr		16
embryo 22-24hr		19
larva L1		29
larva L2		31
larva L3 12hr old		25
larva L3 puffstage 1-2		26
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		22
white prepupae new		23
white prepupae 12hr		24
white prepupae 24hr		23
pupae 2d postWPP		19
pupae 3d postWPP		16
pupae 4d postWPP		13
adult male 01day		18
adult male 05day		18
adult male 30day		16
adult female 01day		25
adult female 05day		40
adult female 30day		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		38
embryonic 1182-4H		16
embryonic GM2		25
embryonic Kc167		24
embryonic S1		26
embryonic S3		27
leg disc CME L1		19
wing disc CME-W2		38
wing disc ML-DmD8		39
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		23
wing disc ML-DmD32		25
haltere disc ML-DmD17-c3		46
eye-antennal disc ML-DmD11		22
antennal disc ML-DmD20-c5		36
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		25
CNS ML-DmBG2-c2		34
tumorous blood cells mbn2		23
ovary fGS/OSS		8
ovary OSC		16
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(42)
Schneider line 2 Sg4		(38)
embryonic 1182-4H		16
embryonic GM2		25
embryonic Kc167		24
embryonic S1		26
embryonic S3		(27)
leg disc CME L1		19
wing disc CME-W2		(38)
wing disc ML-DmD8		(39)
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		23
wing disc ML-DmD32		25
haltere disc ML-DmD17-c3		(46)
eye-antennal disc ML-DmD11		22
antennal disc ML-DmD20-c5		(36)
mixed discs ML-DmD4-c1		(30)
CNS ML-DmBG1-c1		25
CNS ML-DmBG2-c2		(34)
tumorous blood cells mbn2		23
ovary fGS/OSS		8
ovary OSC		16
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		38
embryonic 1182-4H		16
embryonic GM2		25
embryonic Kc167		24
embryonic S1		26
embryonic S3		27
leg disc CME L1		19
wing disc CME-W2		38
wing disc ML-DmD8		39
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		23
wing disc ML-DmD32		25
haltere disc ML-DmD17-c3		46
eye-antennal disc ML-DmD11		22
antennal disc ML-DmD20-c5		36
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		25
CNS ML-DmBG2-c2		34
tumorous blood cells mbn2		23
ovary fGS/OSS		8
ovary OSC		16
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		38
embryonic 1182-4H		16
embryonic GM2		25
embryonic Kc167		24
embryonic S1		26
embryonic S3		27
leg disc CME L1		19
wing disc CME-W2		38
wing disc ML-DmD8		39
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		23
wing disc ML-DmD32		25
haltere disc ML-DmD17-c3		46
eye-antennal disc ML-DmD11		22
antennal disc ML-DmD20-c5		36
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		25
CNS ML-DmBG2-c2		34
tumorous blood cells mbn2		23
ovary fGS/OSS		8
ovary OSC		16
ovary OSS		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		38
embryonic 1182-4H		16
embryonic GM2		25
embryonic Kc167		24
embryonic S1		26
embryonic S3		27
leg disc CME L1		19
wing disc CME-W2		38
wing disc ML-DmD8		39
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		23
wing disc ML-DmD32		25
haltere disc ML-DmD17-c3		46
eye-antennal disc ML-DmD11		22
antennal disc ML-DmD20-c5		36
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		25
CNS ML-DmBG2-c2		34
tumorous blood cells mbn2		23
ovary fGS/OSS		8
ovary OSC		16
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(42)
Schneider line 2 Sg4		(38)
embryonic 1182-4H		16
embryonic GM2		25
embryonic Kc167		24
embryonic S1		26
embryonic S3		27
leg disc CME L1		19
wing disc CME-W2		(38)
wing disc ML-DmD8		(39)
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		23
wing disc ML-DmD32		25
haltere disc ML-DmD17-c3		(46)
eye-antennal disc ML-DmD11		22
antennal disc ML-DmD20-c5		(36)
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		25
CNS ML-DmBG2-c2		(34)
tumorous blood cells mbn2		23
ovary fGS/OSS		8
ovary OSC		16
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		38
embryonic 1182-4H		16
embryonic GM2		25
embryonic Kc167		24
embryonic S1		26
embryonic S3		27
leg disc CME L1		19
wing disc CME-W2		38
wing disc ML-DmD8		39
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		23
wing disc ML-DmD32		25
haltere disc ML-DmD17-c3		46
eye-antennal disc ML-DmD11		22
antennal disc ML-DmD20-c5		36
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		25
CNS ML-DmBG2-c2		34
tumorous blood cells mbn2		23
ovary fGS/OSS		8
ovary OSC		16
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		42
Schneider line 2 Sg4		38
embryonic 1182-4H		16
embryonic GM2		25
embryonic Kc167		24
embryonic S1		26
embryonic S3		27
leg disc CME L1		19
wing disc CME-W2		38
wing disc ML-DmD8		39
wing disc ML-DmD9		19
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		23
wing disc ML-DmD32		25
haltere disc ML-DmD17-c3		46
eye-antennal disc ML-DmD11		22
antennal disc ML-DmD20-c5		36
mixed discs ML-DmD4-c1		30
CNS ML-DmBG1-c1		25
CNS ML-DmBG2-c2		34
tumorous blood cells mbn2		23
ovary fGS/OSS		8
ovary OSC		16
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		3
cold shock, 4-day adult		1
heat shock, 4-day adult		9
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		13
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		9
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		6
Ethanol 2.5% 3 hrs, larvae L3		12
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		15
Paraquat 10 mM 48 hrs, 4-day adult		12
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		3
cold shock, 4-day adult		1
heat shock, 4-day adult		9
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		13
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		9
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		6
Ethanol 2.5% 3 hrs, larvae L3		12
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		15
Paraquat 10 mM 48 hrs, 4-day adult		12
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		3
cold shock, 4-day adult		1
heat shock, 4-day adult		9
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		13
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		9
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		6
Ethanol 2.5% 3 hrs, larvae L3		12
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		15
Paraquat 10 mM 48 hrs, 4-day adult		12
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		3
cold shock, 4-day adult		1
heat shock, 4-day adult		9
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		13
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		9
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		6
Ethanol 2.5% 3 hrs, larvae L3		12
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		15
Paraquat 10 mM 48 hrs, 4-day adult		12
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		3
cold shock, 4-day adult		1
heat shock, 4-day adult		9
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		13
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		9
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		6
Ethanol 2.5% 3 hrs, larvae L3		12
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		15
Paraquat 10 mM 48 hrs, 4-day adult		12
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		3
cold shock, 4-day adult		1
heat shock, 4-day adult		9
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		13
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		9
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		6
Ethanol 2.5% 3 hrs, larvae L3		12
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		15
Paraquat 10 mM 48 hrs, 4-day adult		12
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		3
cold shock, 4-day adult		1
heat shock, 4-day adult		9
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		13
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		9
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		6
Ethanol 2.5% 3 hrs, larvae L3		12
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		15
Paraquat 10 mM 48 hrs, 4-day adult		12
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		3
cold shock, 4-day adult		1
heat shock, 4-day adult		9
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		13
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		9
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		6
Ethanol 2.5% 3 hrs, larvae L3		12
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		10
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		15
Paraquat 10 mM 48 hrs, 4-day adult		12
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0037305

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG12173-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

CG12173^GD7515

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of CG12173	Class	Mutagen	Stocks	Known lesion
CG12173^KG00232			1	--
CG12173^EP614		P-element activity	0	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG12173	Class	Mutagen	Stocks	Known lesion
CG12173^GD7515		in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7515}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}CG12173^EP614

miscellaneous insertions

P{SUPor-P}CG12173^KG00232

Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 4 terms )

Molecular Function

CV term

Evidence

References

acireductone synthase activity

inferred from electronic annotation with InterPro:IPR010041

(FlyBase Curators et al., 2004-)

magnesium ion binding

inferred from electronic annotation with InterPro:IPR023943

(FlyBase Curators et al., 2004-)

phosphoglycolate phosphatase activity

inferred from electronic annotation with InterPro:IPR006439

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

L-methionine salvage from methylthioadenosine

inferred from electronic annotation with InterPro:IPR010041

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG12173 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

65843

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0037305

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

40630

Orthologs

OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6GQQRF

Dipteran inclusive ortholog search

EOG6GHZZB

Insect inclusive ortholog search

EOG6DV4HK

Arthropod inclusive ortholog search

EOG6MSBDK

Metazoa inclusive ortholog search

EOG6RRK9S

Orthologs in Drosophila Species (EOG6GQQRF)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11453

Drosophila persimilis

Dper\GL22195

Drosophila willistoni

Dwil\GK14286

Drosophila virilis

Dvir\GJ11016

Drosophila mojavensis

Dmoj\GI10215

Drosophila grimshawi

Dgri\GH22720

Orthologs in non-Drosophila Dipterans (EOG6GHZZB)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000109

Anopheles gambiae

Malaria mosquito

Agam\AGAP003331

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ009638

Orthologs in non-Dipteran Insects (EOG6DV4HK)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB52285

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011261

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH12096

Atta cephalotes

Leafcutter ant

Acep\ACEP10090

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO20604

Harpegnathos saltator

Jerdons jumping ant

Hsal\mtnC

Linepithema humile

Argentine ant

Lhum\LH25484

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB24495

Solenopsis invicta

Red fire ant

Sinv\SINV18659

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008421

Pediculus humanus

Human body louse

Phum\PHUM452350

Tribolium castaneum

Red flour beetle

Tcas\TC030649

Orthologs in non-Insect Arthropods (EOG6MSBDK)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\Theg

Daphnia pulex

Water flea

Dpux\Snrpg

Orthologs in non-Arthropod Metazoa (EOG6RRK9S)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\F58H1.3

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_016247gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_026712gn

Danio rerio

Zebrafish

Drer\enoph1

Xenopus tropicalis

Western clawed frog

Xtro\enoph1

Gallus gallus

Domestic chicken

Ggal\ENOPH1

Mus musculus

House mouse

Mmus\Enoph1

Rattus norvegicus

Norway rat

Rnor\Enoph1

Homo sapiens

Human

Hsap\ENOPH1

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\ENOPH1

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11453

Drosophila persimilis

Dper\GL22195

Drosophila willistoni

Dwil\GK14286

Drosophila virilis

Dvir\GJ11016

Drosophila mojavensis

Dmoj\GI10215

Drosophila grimshawi

Dgri\GH22720

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Bloomington

13647

y¹ w^67c23; P{SUPor-P}CG12173^KG00232

VDRC

v31674

w¹¹¹⁸; P{GD7515}v31674

v31676

w¹¹¹⁸; P{GD7515}v31676

Genomic Clones ( 1 )

BACR01D10

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 8 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0037305

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

40630

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_141266

RefSeq (Proteins)

•

NP_649523

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

40630

UniProtKB/Swiss-Prot

•

Q9VN95

UniProtKB/TrEMBL

•

E1JJ50

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Haloacid dehalogenase/epoxide hydrolase (IPR005833)

HAD-superfamily hydrolase, subfamily IA, variant 1 (IPR006439)

Enolase-phosphatase E1 (IPR023943)

Enolase-phosphatase E1, eukaryotes (IPR027511)

HAD-like domain (IPR023214)

Linkouts

BioGRID - A database of protein and genetic interactions

•

65843

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0037305

DRSC - Results from RNAi screens.

•

FBgn0037305

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0037305

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG12173-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0037305

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

40630

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

40630

modMine - Data generated by the modENCODE project.

•

FBgn0037305

Synonyms & Secondary IDs ( 1 )

Reported As

Symbol Synonym

CG12173

(Dubruille et al., 2009)

Name Synonym

Secondary FlyBase IDs

References ( 14 )

Research paper

Dubruille et al., 2009, PLoS Genet. 5(12): e1000787: A constant light-genetic screen identifies KISMET as a regulator of circadian photoresponses. [FBrf0209551]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Gene Disruption Project members, 2001-, (Computer file): (Computer file) [FBrf0132177]
Mount, 2001.8.14, Intron annotations.: Intron annotations. [FBrf0137489]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

FlyBase analysis

FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.: Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 1992-, FlyBase curation.: FlyBase curation. [FBrf0105495]

Curated genome annotation

Kodira, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126678]