FlyBase Gene Report: Dmel\NPFR

General Information

Symbol

Dmel\NPFR

Species

D. melanogaster

Name

Neuropeptide F receptor

Annotation Symbol

CG1147

Feature Type

protein_coding_gene

FlyBase ID

FBgn0037408

Gene Model Status

Current

Stock Availability

35 publicly available

Gene Summary

Receptor for NPF. Integral part of the sensory system that mediates food signaling, providing the neural basis for the regulation of food response; coordinates larval foraging and social behavior changes during development. Required in dopaminergic (DA) neurons that innervate the mushroom body for satiety to suppress appetitive memory performance; a key factor in the internal state of hunger in the brain. NPF neurons coordinately modulate diverse sensory and motor neurons important for feeding, flight, and locomotion. NPF/NPFR pathway exerts its suppressive effect on larval aversion to diverse stressful stimuli (chemical stress and noxious heat) through attenuation of TRP channel-induced neuronal excitation. NPF neural signaling system plays a physiological role in acute modulation of alcohol sensitivity in adults, rather than a general response to intoxication by sedative agents. Activation and inhibition of the NPF system reduces and enhances ethanol preference, respectively. Sexual experience, the NPF system activity and ethanol consumption are all linked; sexual deprivation is a major contributor to enhanced ethanol preference. (UniProt, Q9VNM1)

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All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

NPFR1, NPF-R, DmNPFR1

Key Links

Genomic Location

Cytogenetic map

83D5-83E1

Sequence location

3R:6,187,315..6,195,403 [+]

Recombination map

3-47.5

RefSeq locus

NT_033777 REGION:6187315..6195403

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables neuropeptide binding

inferred from physical interaction with FLYBASE:NPF; FB:FBgn0027109

(Garczynski et al., 2005)

enables neuropeptide F receptor activity

inferred from physical interaction with FLYBASE:NPF; FB:FBgn0027109

(Johnson et al., 2003, Garczynski et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables G protein-coupled receptor activity

inferred from electronic annotation with InterPro:IPR000276

(InterPro Project Members, 2004-)

enables neuropeptide receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN002538187

(GO Reference Genome Project, 2011-)

enables neuropeptide Y receptor activity

inferred from electronic annotation with InterPro:IPR000611

(InterPro Project Members, 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in larval feeding behavior

inferred from mutant phenotype

(Wu et al., 2005)

involved_in neuropeptide signaling pathway

inferred from direct assay

(Johnson et al., 2003, Garczynski et al., 2002)

involved_in olfactory behavior

inferred from mutant phenotype

(Lee et al., 2017)

involved_in response to odorant

inferred from mutant phenotype

(Lee et al., 2017)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in G protein-coupled receptor signaling pathway

inferred from sequence model

(Hewes and Taghert, 2001)

inferred from electronic annotation with InterPro:IPR000276, InterPro:IPR000611

(InterPro Project Members, 2004-)

involved_in neuropeptide signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN002538187

(GO Reference Genome Project, 2011-)

involved_in olfactory behavior

inferred from biological aspect of ancestor with PANTHER:PTN001201512

(GO Reference Genome Project, 2011-)

involved_in tachykinin receptor signaling pathway

inferred from sequence or structural similarity with FLYBASE:RYa-R; FB:FBgn0004842

(Brody and Cravchik, 2000)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Johnson et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from sequence or structural similarity with FLYBASE:RYa-R; FB:FBgn0004842

(Brody and Cravchik, 2000)

inferred from sequence model

(Garczynski et al., 2002, Hewes and Taghert, 2001)

inferred from electronic annotation with InterPro:IPR000276, InterPro:IPR000611, InterPro:IPR017452

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN002538187

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CLASS A GPCR NEUROPEPTIDE AND PROTEIN HORMONE RECEPTORS

Protein Family (UniProt)

Belongs to the G-protein coupled receptor 1 family. (Q9VNM1)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CLASS A GPCR NEUROPEPTIDE AND PROTEIN HORMONE RECEPTORS -: The majority of neuropeptide/protein hormone GPCR belong to the Class A family of GPCRs. The secreted ligands for this subfamily include neuropeptides, peptide hormones and protein hormones. (Adapted from FBrf0195077).

Protein Function (UniProtKB)

(UniProt, Q9VNM1)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\NPFR for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VNM1)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.39

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

NPFR-RA

FBtr0078590

NM_079521

3214

485

NPFR-RB

FBtr0308316

NM_001260016

2433

466

NPFR-RC

FBtr0308317

NM_001260017

2465

462

NPFR-RD

FBtr0308318

NM_001260018

2502

489

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

NPFR-PA

FBpp0078239

53.5

485

8.28

FBpp0300635

51.6

466

8.38

FBpp0300636

51.0

462

8.52

FBpp0300637

54.1

489

8.15

A0A0B4K601

NP_001246947

AFH06266

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\NPFR using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

NPFR_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.76

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic central brain

(Fisher et al., 2012)

embryonic central brain glial cell

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

embryonic lateral cord

(Fisher et al., 2012)

ventral midline of embryo

(Fisher et al., 2012)

larval midline neuron

(Kearney et al., 2004)

embryonic stage 14

larval H-cell

(Stagg et al., 2011)

embryonic stage 17

larval H-cell

(Wheeler et al., 2006)

late third instar larval stage

larval brain | restricted

(Garczynski et al., 2002)

larval gnathal ganglion

(Garczynski et al., 2002)

larval ventral nerve cord

(Garczynski et al., 2002)

embryonic/larval midgut

(Garczynski et al., 2002)

Additional Descriptive Data

NPFR transcript is expressed in many cells of the brain lobes and ventral nerve cord of the larval central nervous system. NPFR transcript is also expressed in scattered isolated cells in the larval midgut.

(Garczynski et al., 2002)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

Malpighian tubule

(Veenstra et al., 2008)

adult hindgut

(Veenstra et al., 2008)

adult midgut

(Veenstra et al., 2008)

crop

(Veenstra et al., 2008)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Johnson et al., 2003)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\NPFR in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\NPFR

View exonic expression by tissue for Dmel\NPFR

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0037408

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40754/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037408

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037408

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037408

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1147

Flygut - An atlas of the Drosophila adult midgut

FBgn0037408

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\NPFR

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of NPFR

Transgenic constructs containing regulatory region of NPFR

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

NPFR^c01896

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

NPFR^GD668

viable, with Scer\GAL4^Mef2.PR

NPFR^GD668

viable, with Scer\GAL4^pnr-MD237

NPFR^GD668

viable, with Scer\GAL4^tin.CΔ4

NPFR^GD668

Sterility

Allele

fertile

NPFR^c01896

Other Phenotypes

Allele

abnormal behavior | male, with Scer\GAL4^elav.PU

NPFR^RNAi.UAS

abnormal courtship behavior

NPFR^lexA

abnormal courtship behavior, with Scer\GAL4^elav.PLu

NPFR^KK112704

abnormal courtship behavior (with NPFR^c01896)

NPFR^lexA

abnormal courtship behavior (with NPFR^lexA)

NPFR^c01896

abnormal feeding behavior, with Scer\GAL4^NPFR.6.6

abnormal memory

NPFR^c01896

abnormal memory, with Scer\GAL4^c061

NPFR^RNAi.UAS

abnormal memory, with Scer\GAL4^c061, Scer\GAL80^ple.PK

NPFR^RNAi.UAS

abnormal memory, with Scer\GAL4^nSyb.PS

NPFR^RNAi.UAS

abnormal memory | larval stage | nutrition conditional

NPFR^c01896

abnormal neurophysiology | adult stage

NPFR^c01896

abnormal neurophysiology | adult stage, with Scer\GAL4^Or22a.7.717

NPFR^GD668

abnormal neurophysiology | adult stage, with Scer\GAL4^Orco.PK

NPFR^GD668

abnormal neurophysiology | adult stage (with Df(3R)Tpl3)

NPFR^c01896

abnormal starvation stress response | adult stage, with Scer\GAL4^Akh.PL

abnormal starvation stress response | adult stage, with Scer\GAL4^Ilp2.PR

NPFR^JF01959

abnormal starvation stress response | adult stage, with Scer\GAL4^NP1624

NPFR^JF01959

abnormal starvation stress response | adult stage (with Df(3R)BSC464)

NPFR^sk8

chemical resistant, with Scer\GAL4^unspecified

NPFR^RNAi.UAS

decreased cell number | oogenesis | conditional, with Scer\GAL4^C587

NPFR^KK112704

decreased cell number | oogenesis | conditional, with Scer\GAL4^e22c

NPFR^KK112704

decreased cell number | oogenesis | conditional, with Scer\GAL4^NP1624

decreased cell number | oogenesis | conditional (with Df(3R)BSC464)

NPFR^sk8

decreased fecundity, with Scer\GAL4^NP1624

NPFR^KK112704

decreased occurrence of cell division | oogenesis | conditional, with Scer\GAL4^NP1624

NPFR^KK112704

increased cell number | oogenesis | conditional, with Scer\GAL4^NP1624

NPFR^UAS.cYa

increased rate of feeding behavior | adult stage, with Scer\GAL4^Akh.PL

NPFR^JF01959

increased rate of feeding behavior | adult stage, with Scer\GAL4^Ilp2.PR

NPFR^JF01959

increased rate of movement | adult stage, with Scer\GAL4^elav.PU

NPFR^JF01959

Phenotype manifest in

Allele

adult insulin secreting cell of pars intercerebralis, with Scer\GAL4^Ilp2.PR

NPFR^JF01959

adult olfactory receptor neuron Or22

NPFR^c01896

adult olfactory receptor neuron Or22 (with Df(3R)Tpl3)

NPFR^c01896

adult olfactory receptor neuron Or22 | adult stage, with Scer\GAL4^Or22a.7.717

NPFR^GD668

adult olfactory receptor neuron Or22 | adult stage, with Scer\GAL4^Orco.PK

NPFR^GD668

female germline stem cell, with Scer\GAL4^C587

NPFR^KK112704

female germline stem cell, with Scer\GAL4^e22c

NPFR^KK112704

female germline stem cell, with Scer\GAL4^NP1624

NPFR^KK112704

female germline stem cell | conditional, with Scer\GAL4^NP1624

female germline stem cell | conditional (with Df(3R)BSC464)

NPFR^sk8

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (108)

Hsap\NPY1R

5 of 14

Yes

Hsap\NPY5R

5 of 14

Yes

Hsap\NPY4R

4 of 14

Yes

Hsap\NPY2R

3 of 14

Hsap\NPY4R2

3 of 14

Yes

Hsap\NPY6R

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\AVPR1B

1 of 14

Yes

Hsap\AVPR2

1 of 14

Hsap\BDKRB1

1 of 14

Yes

Hsap\BDKRB2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\CCR2

1 of 14

Yes

Hsap\CCR3

1 of 14

Yes

Hsap\CCR4

1 of 14

Yes

Hsap\CCR5

1 of 14

Yes

Hsap\CCR8

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\CXCR3

1 of 14

Yes

Hsap\CXCR4

1 of 14

Yes

Hsap\CXCR5

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\EDNRB

1 of 14

Yes

Hsap\FPR2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\GPR25

1 of 14

Hsap\GPR37

1 of 14

Yes

Hsap\GPR37L1

1 of 14

Yes

Hsap\GPR45

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\OXTR

1 of 14

Yes

Hsap\P2RY8

1 of 14

Yes

Hsap\PROKR1

1 of 14

Yes

Hsap\PROKR2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (56)

Rnor\Npy1r

6 of 14

Yes

Rnor\Npy5r

6 of 14

Yes

Rnor\Npy4r

3 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Brs3

1 of 14

Rnor\Cckar

1 of 14

Rnor\Ccr2

1 of 14

Yes

Rnor\Ccr3

1 of 14

Yes

Rnor\Ccr8

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Vom1r3

1 of 14

Yes

Rnor\Vom1r4

1 of 14

Yes

Rnor\Vom1r15

1 of 14

Yes

Rnor\Vom1r29

1 of 14

Yes

Rnor\Vom1r44

1 of 14

Yes

Rnor\Vom1r47

1 of 14

Yes

Rnor\Vom1r52

1 of 14

Yes

Rnor\Vom1r53

1 of 14

Yes

Rnor\Vom1r63

1 of 14

Yes

Rnor\Vom1r107

1 of 14

Yes

Rnor\Vom1r108

1 of 14

Yes

Mus musculus (laboratory mouse) (58)

Mmus\Npy1r

6 of 14

Yes

Mmus\Npy4r

5 of 14

Yes

Mmus\Npy5r

5 of 14

Yes

Mmus\Npy6r

5 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Ccr5

1 of 14

Yes

Mmus\Ccr8

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gpr19

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Vmn1r71

1 of 14

Yes

Mmus\Vmn1r72

1 of 14

Yes

Mmus\Vmn1r75

1 of 14

Yes

Mmus\Vmn1r76

1 of 14

Yes

Mmus\Vmn1r77

1 of 14

Yes

Mmus\Vmn1r78

1 of 14

Yes

Mmus\Vmn1r80

1 of 14

Yes

Mmus\Vmn1r81

1 of 14

Yes

Mmus\Vmn1r83

1 of 14

Yes

Mmus\Vmn1r177

1 of 14

Yes

Mmus\Vmn1r185

1 of 14

Yes

Mmus\Vmn1r224

1 of 14

Yes

Mmus\Vmn1r231

1 of 14

Yes

Mmus\Xcr1

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (90)

Xtro\npy5r

6 of 13

Yes

Xtro\npy4r

3 of 13

Yes

Xtro\npy1r

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\avpr2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ccr3

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\f2rl3

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100489992

1 of 13

Xtro\LOC100490372

1 of 13

Xtro\LOC100494352

1 of 13

Yes

Xtro\LOC100498398

1 of 13

Yes

Xtro\LOC101733812

1 of 13

Yes

Xtro\LOC101733830

1 of 13

Yes

Xtro\LOC105946314

1 of 13

Yes

Xtro\LOC105946838

1 of 13

Xtro\LOC116411706

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xtro\opnpl [provisonal]

1 of 13

Xtro\oprk1

1 of 13

Xtro\qrfprl2.2

1 of 13

Yes

Xtro\qrfpr

1 of 13

Yes

Xtro\rho

1 of 13

Yes

Xtro\rrh

1 of 13

Xtro\shisal2b

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tmtops

1 of 13

Yes

Xtro\topaz1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (68)

Drer\npy4r

7 of 14

Yes

Drer\npy8ar

7 of 14

Yes

Drer\npy8br

7 of 14

Yes

Drer\npy1r

6 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Drer\si:dkey-202l22.3

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\prlhr2b

1 of 14

Drer\qrfprb

1 of 14

Yes

Drer\si:ch211-207g17.3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (57)

Cele\npr-11

12 of 14

Yes

Cele\npr-12

7 of 14

Yes

Cele\npr-1

2 of 14

Yes

Cele\npr-2

2 of 14

Yes

Cele\npr-4

2 of 14

Cele\npr-7

2 of 14

Cele\npr-8

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (40)

Agam\AgaP_AGAP004122

12 of 12

Yes

Agam\GPRNPY2

12 of 12

Yes

Agam\GPRNPY3

2 of 12

Agam\AgaP_AGAP005356

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:NPFR. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (44)

4 of 13

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Lkr

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Rh2

1 of 13

Rh4

1 of 13

Rh7

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

NPFR^RNAi.UAS

model of alcohol use disorder

CEA

(Shohat-Ophir et al., 2012)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NPY1R; neuropeptide Y receptor Y1

5 of 14

162641

NPY5R; neuropeptide Y receptor Y5

5 of 14

602001

NPY4R; neuropeptide Y receptor Y4

4 of 14

601790

NPY2R; neuropeptide Y receptor Y2

3 of 14

162642

NPY4R2; neuropeptide Y receptor Y4-2

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

NPFR - NPF

phenotype-based detection assay, fluorescence microscopy, inferred by author

(Johnson et al., 2003)

RNA-RNA

Physical Interaction

Assay

References

NPFR - mir-276a

luminiscence technology, western blot

(Zhang et al., 2021)

NPFR - mir-276b

luminiscence technology

(Zhang et al., 2021)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

NPFR

suppressible

Akh

(Yoshinari et al., 2021)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0037408

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47.5

Cytogenetic map

83D5-83E1

Sequence location

3R:6,187,315..6,195,403 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

83D5-83E1

Limits computationally determined from genome sequence between P{lacW}cas^j1C2 and P{PZ}lab⁰¹²⁴¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-47.5

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (35)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

84550

w^*; TI{TI}NPFR^attP

Korea

13775

P{VT037213-GAL4}attP2

13776

P{VT037214-GAL4}attP2

16008

P{GMR60E02-lexA}JK22C

17606

PBac{GMR60E02-lexA}VK00027

16308

P{GMR60E02-lexA}su(Hw)attP8

17388

P{VT037213-lexA::GADfl}attP2

17152

P{VT037213-lexA::GADfl}attP40

1147R-1

1147R-3

v107663

v9605

More stocks available...

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (7)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE18294

Other clones

FI03245

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0037408

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0037408

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Xu et al., 2010)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.

(McDonald and Rosbash, 2001)

Relationship to Other Genes

Source for database merge of

Source for merge of: NPFR1 anon-WO0131005.3 anon-WO0131005.5

(FlyBase, 1996-)

Additional comments

Source for merge of NPFR1 anon-WO0131005.3 anon-WO0131005.5 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: NPFR1 CG1147

(Garczynski, 2001.3.23)

Source for identity of: NPFR NPFR1

(FlyBase, 2009-)

Nomenclature comments

Symbol changed from 'NPFR1' to 'NPFR' as the '1' suffix is superfluous.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (23)

Reported As

Symbol Synonym

DmNPFR1

(Nässel and Wegener, 2011, Garczynski et al., 2002)

Drm-NPFR

(Garczynski et al., 2005)

NPF R

(Qi et al., 2021)

NPF receptor

(Honda, 2022)

NPF-R

(Rasmussen et al., 2015, Bass et al., 2014)

NPF-R1

(Top and Young, 2018)

NPFR

(Asahina and Zelikowsky, 2025, Nässel, 2025, Wang et al., 2025, Ryvkin et al., 2024, Bossen et al., 2023, Chen et al., 2023, Hu et al., 2023, He et al., 2022, Nässel and Wu, 2022, Palavicino-Maggio and Sengupta, 2022, Schlichting et al., 2022, White and Wolfner, 2022, Barber et al., 2021, Feng et al., 2021, Hoshino and Niwa, 2021, Hung et al., 2021, Kannangara et al., 2021, Liu et al., 2021, McLaughlin et al., 2021, Nässel and Wu, 2021, Sadanandappa et al., 2021, Sareen et al., 2021, Yoshinari et al., 2021, Kannangara et al., 2020, Kondo et al., 2020, Lathen et al., 2020, Rizzo and Johnson, 2020, Deng et al., 2019, Hegazi et al., 2019, Lin et al., 2019, Rao and Deng, 2019.10.23, Zhang et al., 2019, Ameku et al., 2018, Kim et al., 2017, Lee et al., 2017, Liang et al., 2017, Wang et al., 2016, Yoon et al., 2016, Grotewiel and Bettinger, 2015, Amcheslavsky et al., 2014, Erion and Sehgal, 2013, Nässel et al., 2013, Shohat-Ophir et al., 2012, Feng et al., 2003)

NPFR-1

(Bowser and Tobe, 2006)

NPFr

(Shang et al., 2013)

RE18294p

(Metpally and Sowdhamini, 2005)

anon-WO0131005.3

anon-WO0131005.5

dNPFR1

(Sokolowski, 2003)

npfr

(Chouhan et al., 2021, Guevara et al., 2018)

npfr1

(Zhang et al., 2021, Zhang et al., 2021, Yurgel et al., 2019, He et al., 2013, He et al., 2013, Krashes et al., 2009, Wen et al., 2005, Wu et al., 2003)

Name Synonyms

NPF-receptor

(Veenstra et al., 2008)

Neuropeptide F Receptor 1

(Shah et al., 2012)

Neuropeptide F receptor

(Yu et al., 2016, Caers et al., 2012)

dNPF receptor

(Krashes et al., 2009)

neuropeptide F receptor

(Chintapalli et al., 2012, Guardiola and Crews, 2007, Wheeler et al., 2006, Wu et al., 2005)

neuropeptide F receptor-1

(Lingo et al., 2007)

Secondary FlyBase IDs

FBgn0044358
FBgn0044359

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 46 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40754

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VNM1

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VNM1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VNM1

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0037408

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40754/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037408

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037408

FlyMine - An integrated database for Drosophila genomics

FBgn0037408

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1147

MARRVEL_MODEL - MARRVEL (model organism gene)

40754

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0037408

DroID - A comprehensive database of gene and protein interactions.

FBgn0037408

DRSC - Results frm RNAi screens

FBgn0037408

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

NPFR_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037408

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0037408

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1147

Flygut - An atlas of the Drosophila adult midgut

FBgn0037408

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034469

References (163)

The following external sites may use different assemblies or annotations than FlyBase.

-0.76

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