FlyBase Gene Report: Dmel\Osi8

General Information

Symbol

Dmel\Osi8

Species

D. melanogaster

Name

Osiris 8

Annotation Symbol

CG15591

Feature Type

protein_coding_gene

FlyBase ID

FBgn0037415

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

83E2-83E2

Sequence location

3R:6,255,175..6,256,602 [+]

Recombination map

3-47.5

RefSeq locus

NT_033777 REGION:6255175..6256602

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in membrane

inferred from biological aspect of ancestor with PANTHER:PTN000498286

(GO Reference Genome Project, 2011-)

located_in plasma membrane

inferred from sequence model

(Dorer et al., 2003)

Gene Group (FlyBase)

OSIRIS

Protein Family (UniProt)

Protein Signatures (InterPro)

Protein of unknown function DUF1676

Summaries

Gene Group (FlyBase)

OSIRIS -: The Osiris gene cluster is present in insects and encodes a family of well conserved putative transmembrane proteins of unknown function. In D. melanogaster, most of the genes are clustered within the uniquely dosage-sensitive Triplo-lethal (Tpl) locus. Flies with either one copy or three copies of Tpl die as late embryos or early first instar larvae. (Adapted from FBrf0173211 and FBrf0217655).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Osi8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VNM8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Osi8-RA

FBtr0078598

NM_141370

1135

274

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Osi8-PA

FBpp0078247

29.4

274

10.11

Q9VNM8

NP_649627

AAF51901

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Protein of unknown function DUF1676

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VNM8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Osi8 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Osi8_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.21

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

trichoid sensillum of antennal segment 3

(Scalzotto et al., 2022)

tormogen cell of trichoid sensillum of antennal segment 3

(Scalzotto et al., 2022)

Additional Descriptive Data

Osi8 is expressed in tormogen cells of the antennal trichoid sensillum.

(Scalzotto et al., 2022)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Osi8 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Osi8

View exonic expression by tissue for Dmel\Osi8

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40762/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037415

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037415

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037415

Flygut - An atlas of the Drosophila adult midgut

FBgn0037415

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Osi8

Transgenic constructs containing regulatory region of Osi8

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

Osi8^KK107221

viable

Osi8¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Osi8^GD2823

viable, with Scer\GAL4^Act5C.PU

Osi8^NIG.15591R

viable, with Scer\GAL4^da.G32

Osi8^NIG.15591R

viable, with Scer\GAL4^neur-P72

Osi8^NIG.15591R

viable, with Scer\GAL4^pnr-MD237

Osi8^GD2823

Sterility

Allele

fertile

Osi8¹

Other Phenotypes

Allele

abnormal neurophysiology | adult stage, with Scer\GAL4^Act5C.PI

Osi8^NIG.15591R

abnormal neurophysiology | adult stage | conditional

Osi8¹

Phenotype manifest in

Allele

adult olfactory receptor neuron Or47b | adult stage

Osi8¹

adult olfactory receptor neuron Or47b | adult stage, with Scer\GAL4^Act5C.PI

Osi8^NIG.15591R

adult olfactory receptor neuron Or88a | adult stage

Osi8¹

adult olfactory receptor neuron Or88a | adult stage, with Scer\GAL4^Act5C.PI

Osi8^NIG.15591R

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (21)

Agam\AgaP_AGAP004128

11 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Osi8. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (23)

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

65965

DroID - A comprehensive database of gene and protein interactions.

FBgn0037415

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47.5

Cytogenetic map

83E2-83E2

Sequence location

3R:6,255,175..6,256,602 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

83E2-83E2

Limits computationally determined from genome sequence between P{lacW}cas^j1C2 and P{PZ}lab⁰¹²⁴¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-47.5

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

15591R-1

v100240

v5753

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (2)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP24490

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0037415

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

DMG1-chr3R.3.014.a

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0037415

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

The Tpl region is both triplo-lethal and haplo-lethal and neither point nor insertional mutations of Tpl have been isolated despite extensive mutagenesis analysis. This raises the possibility that Tpl consists of a cluster of genes with at least partial redundancy. Osi8 is a member of the "Osiris" family of genes (which contains 23 members). 20 of the "Osiris" genes (including Osi8) are located in a cluster within the Tpl region of the genome at 83D-83E (the remaining 3 members - Osi21, Osi22 and Osi23 - are located at three different sites in the genome. None of these 3 loci are triplo-lethal or are located within a haplo-insufficient region). The "Osiris" gene family is also present in the A.gambiae genome and shows remarkable synteny and sequence conservation with the D.melanogaster cluster. There appears to have been selection on the "Osiris" family both for expression level and function. It is suggested that the "Osiris" gene family cluster corresponds to Tpl.

(Dorer et al., 2003)

Nomenclature History

Source for database identify of

Source for identity of: Osi8 CG15591

(Dorer et al., 2003)

Nomenclature comments

Etymology

The gene is named "Osiris" since it belongs to a family of related proteins, 20 of which are in a cluster located in the Tpl region, and the "Isis" locus partially rescues the effect of trisomy for this region.

(Dorer et al., 2003)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG15591

(Keleman et al., 2009.8.5, Bhutkar et al., 2008, Quinones-Coello, 2007, Dorer et al., 2003, Zdobnov et al., 2002)

Osi8

(Lanno et al., 2019, Scholl et al., 2018, Japanese National Institute of Genetics, 2012.5.21, Shah et al., 2012, Bhutkar et al., 2008, Quinones-Coello, 2007)

Name Synonyms

Osiris

(Dorer et al., 2004)

Osiris 8

(Dorer et al., 2003)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 23 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40762

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAF51901

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VNM8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VNM8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VNM8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40762/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037415

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037415

FlyMine - An integrated database for Drosophila genomics

FBgn0037415

InterPro - A database of protein families, domains and functional sites

Protein of unknown function DUF1676

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG15591

MARRVEL_MODEL - MARRVEL (model organism gene)

40762

Linkouts

BioGRID - A database of protein and genetic interactions.

65965

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0037415

DroID - A comprehensive database of gene and protein interactions.

FBgn0037415

DRSC - Results frm RNAi screens

FBgn0037415

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Osi8_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037415

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0037415

Flygut - An atlas of the Drosophila adult midgut

FBgn0037415

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011661

References (26)

The following external sites may use different assemblies or annotations than FlyBase.

-0.21

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