FlyBase Gene Report: Dmel\CG10298

General Information

Symbol

Dmel\CG10298

Species

D. melanogaster

Name

Annotation Symbol

CG10298

Feature Type

protein_coding_gene

FlyBase ID

FBgn0037432

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

83E4-83E4

Sequence location

3R:6,343,002..6,343,799 [+]

Recombination map

3-47.5

RefSeq locus

NT_033777 REGION:6343002..6343799

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in negative regulation of ERK1 and ERK2 cascade

inferred from sequence or structural similarity with UniProtKB:Q9VD01

(Rautureau et al., 2009)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in cytosol

inferred from sequence or structural similarity with UniProtKB:Q9VD01

(Rautureau et al., 2009)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG10298 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VI08)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

CG10298 is highly related to adjacent genes CG17917 and CG17919..

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG10298-RA

FBtr0081710

NM_141386

745

187

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG10298-PA

FBpp0081207

20.9

187

9.51

Q9VI08

NP_649643

AAF54137

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VI08

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG10298 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG10298_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

4.88

Transcript Expression

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis

(Rautureau et al., 2009)

Additional Descriptive Data

CG10298 transcript is detected only in the adult testis, and is not detected in embryos.

(Rautureau et al., 2009)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis

(Rautureau et al., 2009)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis

(Rautureau et al., 2009)

Additional Descriptive Data

CG10298 protein is detected only in the adult testis.

(Rautureau et al., 2009)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG10298 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG10298

View exonic expression by tissue for Dmel\CG10298

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40779/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037432

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037432

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037432

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10298

Flygut - An atlas of the Drosophila adult midgut

FBgn0037432

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG10298

Transgenic constructs containing regulatory region of CG10298

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG10298^GD13252

viable, with Scer\GAL4^Mef2.PR

CG10298^GD13252

viable, with Scer\GAL4^pnr-MD237

CG10298^GD13252

viable, with Scer\GAL4^tin.CΔ4

CG10298^GD13252

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (3)

Hsap\PEBP1

13 of 14

Yes

Hsap\PEBP4

7 of 14

Yes

Hsap\MRPL38

3 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (4)

Rnor\Pbp2

12 of 14

Yes

Rnor\Pebp1

12 of 14

Yes

Rnor\Mrpl38

3 of 14

Rnor\Pebp4

1 of 14

Yes

Mus musculus (laboratory mouse) (4)

Mmus\Pbp2

12 of 14

Yes

Mmus\Pebp1

11 of 14

Yes

Mmus\Pebp4

7 of 14

Yes

Mmus\Mrpl38

3 of 14

Xenopus tropicalis (Western clawed frog) (4)

Xtro\pebp1.2

10 of 13

Yes

Xtro\pebp1.1

9 of 13

Xtro\pebp4

5 of 13

Yes

Xtro\mrpl38

2 of 13

Danio rerio (Zebrafish) (3)

Drer\pebp1

12 of 14

Yes

Drer\mrpl38

3 of 14

Drer\pebp4

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\F40A3.3

9 of 14

Yes

Cele\Y69E1A.5

7 of 14

Yes

Cele\mrpl-38

3 of 14

Cele\C26B2.8

1 of 14

Yes

Cele\C56G2.4

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (7)

Agam\AgaP_AGAP012962

7 of 12

Yes

Agam\AgaP_AGAP002049

6 of 12

Agam\AgaP_AGAP003580

6 of 12

Yes

6 of 12

6 of 12

6 of 12

3 of 12

Arabidopsis thaliana (thale-cress) (7)

Atha\E12A11

12 of 13

Yes

Atha\TFL1

12 of 13

Yes

Atha\ATC

11 of 13

Atha\BFT

11 of 13

Yes

Atha\FT

11 of 13

Yes

Atha\TSF

11 of 13

Yes

Atha\AT5G01300

3 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\TFS1

9 of 13

Yes

Scer\YLR179C

9 of 13

Yes

Scer\MRPL35

4 of 13

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC2F12.10

3 of 12

Yes

Escherichia coli (enterobacterium) (2)

Ecol\ybcL

4 of 11

Yes

Ecol\ybhB

4 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG10298. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

CG6180

11 of 13

CG17919

11 of 13

10 of 13

10 of 13

10 of 13

10 of 13

8 of 13

6 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PEBP1; phosphatidylethanolamine binding protein 1

13 of 14

604591

PEBP4; phosphatidylethanolamine binding protein 4

7 of 14

612473

MRPL38; mitochondrial ribosomal protein L38

3 of 14

611844

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

65981

DroID - A comprehensive database of gene and protein interactions.

FBgn0037432

MIST (protein-protein) - An integrated Molecular Interaction Database

40779

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47.5

Cytogenetic map

83E4-83E4

Sequence location

3R:6,343,002..6,343,799 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

83E4-83E4

Limits computationally determined from genome sequence between P{lacW}cas^j1C2 and P{PZ}lab⁰¹²⁴¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-47.5

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

85354

w¹¹¹⁸; PBac{PB}CG10298^c02144

FlyORF

F003096

M{UAS-CG10298.ORF.3xHA.GW}ZH-86Fb

VDRC

v104821

P{KK111601}VIE-260B

v28706

w¹¹¹⁸; P{GD13252}v28706/TM3

Genomic Clones (8)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH28351

Other clones

DPiM_GH28351

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0037432

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0037432

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Rautureau et al., 2009)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG10298 BcDNA:GH28351

(FlyBase, 1996-)

Additional comments

Source for merge of CG10298 BcDNA:GH28351 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

BcDNA:GH28351

CG10298

(Känel et al., 2022, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Rautureau et al., 2009, Langille and Clark, 2007)

Name Synonyms

Secondary FlyBase IDs

FBgn0063214

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 36 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40779

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VI08

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VI08

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VI08

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40779/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037432

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037432

FlyMine - An integrated database for Drosophila genomics

FBgn0037432

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10298

MARRVEL_MODEL - MARRVEL (model organism gene)

40779

Linkouts

BioGRID - A database of protein and genetic interactions.

65981

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0037432

DroID - A comprehensive database of gene and protein interactions.

FBgn0037432

DRSC - Results frm RNAi screens

FBgn0037432

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG10298_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037432

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0037432

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10298

Flygut - An atlas of the Drosophila adult midgut

FBgn0037432

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

MIST (protein-protein) - An integrated Molecular Interaction Database

40779

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (26)

The following external sites may use different assemblies or annotations than FlyBase.

4.88

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