FB2021_01, released Feb 18, 2021

Gene: Dmel\PolI

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General Information
Symbol
Dmel\PolI
Species
D. melanogaster
Name
DNA polymerase iota
Annotation Symbol
CG7602
Feature Type
protein_coding_gene
FlyBase ID
FBgn0037554
Gene Model Status
Current
Stock Availability
4 publicly available
Gene Snapshot
DNA polymerase iota (PolI) encodes a protein involved in translesion synthesis. [Date last reviewed: 2019-09-26]
Other Summaries
Also Known As

DNApol-iota, drad30B, DNApol-ι

Key Links
Genomic Location
Cytogenetic map
84F4-84F5
Sequence location
3R:8,233,507..8,237,389 [+]
Recombination map

3-48

RefSeq locus
NT_033777 REGION:8233507..8237389
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (3 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables DNA-directed DNA polymerase activity
inferred from direct assay
(Ishikawa et al., 2001)
inferred from mutant phenotype
(Ishikawa et al., 2001)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables damaged DNA binding
inferred from electronic annotation with InterPro:IPR017961
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables DNA-directed DNA polymerase activity
inferred from biological aspect of ancestor with PANTHER:PTN000117943
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in translesion synthesis
inferred from direct assay
(Ishikawa et al., 2001)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in translesion synthesis
inferred from sequence or structural similarity with HGNC:9182
(Ishikawa et al., 2001)
inferred from biological aspect of ancestor with PANTHER:PTN000117943
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the DNA polymerase type-Y family. (Q9VHV1)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
DNA POLYMERASE IOTA -
DNA polymerase iota is one of several translesion synthesis polymerases that act to promote replication through DNA lesions that would otherwise stall the replicative polymerases. Instead of Watson-Crick pairing, DNA polymerase iota is specialized to promote Hoogsteen base-pairing (Adapted from PMID:30005324.)
DNA POLYMERASE CATALYTIC SUBUNITS -
Multiple DNA polymerases are required for DNA replication and/or repair in eukaryotic cells. The alpha-primase, delta, epsilon, zeta and gamma polymerases exist as multi-subunit complexes in which one of the subunits has catalytic activity, whereas other DNA polymerases act as single molecules. (Adapted from PMID:30005324.)
Protein Function (UniProtKB)
Error-prone DNA polymerase specifically involved in DNA repair (PubMed:11297519). Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:11297519). Favors Hoogsteen base-pairing in the active site (By similarity). Inserts the correct base with higher fidelity opposite an adenosine template (PubMed:11297519). Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine (PubMed:11297519). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity).
(UniProt, Q9VHV1)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\PolI for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
PolI-RA
FBtr0081798
NM_141515
2622
737
PolI-RB
FBtr0081797
NM_169207
2544
737
PolI-RC
FBtr0339606
NM_001300298
3144
737
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
PolI-PA
FBpp0081294
80.4
737
8.66
NP_649772
AAF54198
PolI-PB
FBpp0081293
80.4
737
8.66
NP_731214
AAN14307
PolI-PC
FBpp0308676
80.4
737
8.66
NP_001287227
AHN57226
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

737 aa isoforms: PolI-PA, PolI-PB, PolI-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Domain

The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.

(UniProt, Q9VHV1)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\PolI using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\PolI in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of PolI
Transgenic constructs containing regulatory region of PolI
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation
Gene partially disrupted in
Df(3R)Ada2bd52
(Pankotai et al., 2005)
Inferred from location
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\POLI
13 of 15
Yes
Yes
Hsap\POLH
3 of 15
No
No
Hsap\POLK
2 of 15
No
Yes
Hsap\ESCO1
1 of 15
No
No
Hsap\ESCO2
1 of 15
No
No
Hsap\REV1
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Poli
13 of 15
Yes
Yes
Mmus\Polh
2 of 15
No
No
Mmus\Polk
2 of 15
No
Yes
Mmus\Esco1
1 of 15
No
No
Mmus\Esco2
1 of 15
No
No
Mmus\Rev1
1 of 15
No
No
Rattus norvegicus (Norway rat) (3)
Rnor\Poli
11 of 13
Yes
Yes
Rnor\Polh
2 of 13
No
No
Rnor\Rev1
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (4)
Xtro\poli
2 of 12
Yes
Yes
Xtro\polk
2 of 12
Yes
Yes
Xtro\polh
1 of 12
No
No
Xtro\rev1
1 of 12
No
No
Danio rerio (Zebrafish) (6)
Drer\poli
12 of 15
Yes
Yes
Drer\polh
4 of 15
No
No
Drer\polk
2 of 15
No
Yes
Drer\esco1
1 of 15
No
No
Drer\esco2
1 of 15
No
No
Drer\rev1
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\polh-1
3 of 15
Yes
No
Cele\polk-1
3 of 15
Yes
Yes
Cele\F08F8.4
1 of 15
No
No
Cele\rev-1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (1)
Atha\POLH
2 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\RAD30
3 of 15
Yes
No
Scer\ECO1
2 of 15
No
No
Scer\REV1
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\eso1
2 of 12
Yes
No
Spom\kpa1
1 of 12
No
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091904LF )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila simulans
Dsim\GD18521
Drosophila sechellia
Dsec\GM23708
Drosophila erecta
Dere\GG23702
Drosophila yakuba
Dyak\GE25854
Drosophila ananassae
Dana\GF18848
Drosophila pseudoobscura pseudoobscura
Dpse\GA20473
Drosophila persimilis
Dper\GL24261
Drosophila willistoni
Dwil\GK14382
Drosophila virilis
Dvir\GJ24165
Drosophila mojavensis
Dmoj\GI23309
Drosophila grimshawi
Dgri\GH18329
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504YU )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17012795
Glossina morsitans
Tsetse fly
Gmor\GMOY004624
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11863
Mayetiola destructor
Hessian fly
Mdes\Mdes000798
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL006715
Anopheles gambiae
Malaria mosquito
Agam\AGAP003157
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ018238
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04LX )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA005125
Danaus plexippus
Monarch butterfly
Dple\DPOGS214594-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL006970
Apis florea
Little honeybee
Aflo\2852197732
Apis mellifera
Western honey bee
Amel\GB40925
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP11385
Bombus terrestris
Buff-tailed bumblebee
Bter\15216235
Linepithema humile
Argentine ant
Lhum\LH12384
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00230e
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG013291
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08874
Tribolium castaneum
Red flour beetle
Tcas\TC008961
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_13125
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04IJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_3781
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G033F )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Poli
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Rad30
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (3)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
PolH
4 of 10
eco
2 of 10
Rev1
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    POLI; DNA polymerase iota
    13 of 15
    605252
        POLH; DNA polymerase eta
        3 of 15
        603968
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        PolI - Rev1
        two hybrid
        (Kosarek et al., 2008)
        PolI - nopo
        two hybrid
        (Wallace et al., 2014)
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map

        3-48

        Cytogenetic map
        84F4-84F5
        Sequence location
        3R:8,233,507..8,237,389 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        84F4-84F5
        Limits computationally determined from genome sequence between P{PZ}grn05930 and P{PZ}Mcm2rL074
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        84E-84F
        (determined by in situ hybridisation)
        (Ishikawa et al., 2001)
        Experimentally Determined Recombination Data
        Location

        3-48

        (FlyBase, 2016)

        3-44.8

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (4)
        Genomic Clones (18)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (210)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: DNApol-ι CG7602

        (FlyBase, 1996-)

        Source for identity of: PolI DNApol-ι

        (Marygold et al., 2020)
        Source for database merge of
        Additional comments

        Source for identity of DNApol-ι CG7602 was sequence comparison ( date:001001 ).

        (FlyBase, 1996-)
        Other Comments

        Transcriptionally downregulated after 2h of insulin stimulation in Kc167 cells. Contains fkh response elements (FHREs) in the genomic upstream or intronic sequences.

        (Junger et al., 2003)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 47 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DPiM - Drosophila Protein interaction map
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (12)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (37)