FB2025_02 , released April 17, 2025
Gene: Dmel\Cks85A
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General Information
Symbol
Dmel\Cks85A
Species
D. melanogaster
Name
Cyclin-dependent kinase subunit 85A
Annotation Symbol
CG9790
Feature Type
protein_coding_gene
FlyBase ID
FBgn0037613
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
Cyclin-dependent kinase subunit 85A (Cks85A) encodes a small protein that interacts with the products of Cdk1 and Cdk2. It also functions with the product of Skp2 to target specific substrates for destruction, including the product of dap. It is required for larval growth and to maintain diploidy in imaginal tissues. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
85A8-85A9
Sequence location
Recombination map
3-48
RefSeq locus
NT_033777 REGION:8712906..8718456
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (10 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
enables cyclin-dependent protein serine/threonine kinase activator activity
inferred from biological aspect of ancestor with PANTHER:PTN000602176
(GO Reference Genome Project, 2011-)
enables cyclin-dependent protein serine/threonine kinase regulator activity
inferred from electronic annotation with InterPro:IPR000789, InterPro:IPR036858
(InterPro Project Members, 2004-)
enables histone binding
inferred from biological aspect of ancestor with PANTHER:PTN000602176
(GO Reference Genome Project, 2011-)
enables protein kinase binding
inferred from biological aspect of ancestor with PANTHER:PTN000602176
(GO Reference Genome Project, 2011-)
enables ubiquitin binding
inferred from biological aspect of ancestor with PANTHER:PTN000602176
(GO Reference Genome Project, 2011-)
Biological Process (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in endomitotic cell cycle
inferred from mutant phenotype
(Ghorbani et al., 2011)
involved_in mitotic cell cycle
inferred from mutant phenotype
(Ghorbani et al., 2011, Pearson et al., 2005)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in regulation of mitotic cell cycle
inferred from biological aspect of ancestor with PANTHER:PTN000602176
(GO Reference Genome Project, 2011-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
part_of SCF ubiquitin ligase complex
inferred from direct assay
(Ghorbani et al., 2011)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of cyclin-dependent protein kinase holoenzyme complex
inferred from biological aspect of ancestor with PANTHER:PTN000602176
(GO Reference Genome Project, 2011-)
part_of SCF ubiquitin ligase complex
inferred from biological aspect of ancestor with PANTHER:PTN000602176
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Cyclin-dependent kinase subunit 85A (Cks85A) encodes a small protein that interacts with the products of Cdk1 and Cdk2. It also functions with the product of Skp2 to target specific substrates for destruction, including the product of dap. It is required for larval growth and to maintain diploidy in imaginal tissues. [Date last reviewed: 2019-03-07]
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Cks85A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VHN1)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.48

Antisense UTR: 3' UTR of one isoform is antisense to CG8112 on opposite strand.

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Cks85A-RA
FBtr0081949
NM_141560
610
96
Cks85A-RB
FBtr0305339
NM_001260063
666
96
Cks85A-RC
FBtr0337074
NM_001275454
2904
96
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Cks85A-PA
FBpp0081430
11.0
96
6.80
Q9VHN1
NP_649817
AAF54272
Cks85A-PB
FBpp0293882
11.0
96
6.80
Q9VHN1
NP_001246992
AFH06310
Cks85A-PC
FBpp0307999
11.0
96
6.80
Q9VHN1
NP_001262383
AGB95765
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

96 aa isoforms: Cks85A-PA, Cks85A-PB, Cks85A-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cks85A using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.71

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of SCF ubiquitin ligase complex
inferred from direct assay
(Ghorbani et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Cks85A in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Cks85A
Transgenic constructs containing regulatory region of Cks85A
Aberrations (Deficiencies and Duplications) ( 2 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die before end of prepupal stage
lethal - all die before end of P-stage
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal developmental rate | larval stage
abnormal endomitotic cell cycle | third instar larval stage
abnormal endomitotic cell cycle | third instar larval stage (with Df(3R)BSC197)
abnormal mitotic cell cycle | third instar larval stage | heat sensitive
increased cell death | third instar larval stage
some die during prepupal stage | recessive
some die during P-stage
visible, with Cks85Aex15, Scer\GAL4en-e16E
visible, with Cks85AGD13737, Scer\GAL4en-e16E
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
embryonic/larval salivary gland, with Scer\GAL4ptc-559.1
embryonic/larval salivary gland | third instar larval stage
embryonic/larval salivary gland | third instar larval stage (with Df(3R)BSC197)
eye disc | third instar larval stage | heat sensitive
larval brain | third instar larval stage
trichogen cell, with Scer\GAL4pnr-MD237
wing disc | third instar larval stage
wing disc | third instar larval stage | heat sensitive
wing hair, with Cks85Aex15, Scer\GAL4en-e16E
wing hair, with Cks85AGD13737, Scer\GAL4en-e16E
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\CKS1B
10 of 14
Yes
No
1  
Hsap\CKS2
9 of 14
No
No
1  
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (4)
Rnor\Cks1b
10 of 14
Yes
No
Rnor\Cks2
9 of 14
No
No
Rnor\Cks1l
7 of 14
No
No
Rnor\LOC120102505
6 of 14
No
No
Mus musculus (laboratory mouse) (3)
Mmus\Cks1b
9 of 14
Yes
No
Mmus\Cks2
9 of 14
Yes
No
Mmus\Cks1brt
8 of 14
No
No
Xenopus tropicalis (Western clawed frog) (4)
Xtro\cks2
3 of 13
Yes
No
Xtro\cks1b
2 of 13
No
Yes
Xtro\secisbp2
2 of 13
No
No
Xtro\hint3
1 of 13
No
No
Danio rerio (Zebrafish) (3)
Drer\cks1b
10 of 14
Yes
No
Drer\cks2
8 of 14
No
No
Drer\zgc:86839
8 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\cks-1
13 of 14
Yes
Yes
Cele\Y71G12B.27
6 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP003796
12 of 12
Yes
Yes
Agam\AgaP_AGAP009442
6 of 12
No
No
Arabidopsis thaliana (thale-cress) (2)
Atha\CKS1
11 of 13
Yes
No
Atha\CKS2
11 of 13
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\CKS1
10 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\suc1
9 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Cks85A. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (3)
Cks30A
11 of 13
CG7006
2 of 13
Sbp2
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    CKS1B; CDC28 protein kinase regulatory subunit 1B
    10 of 14
    116900
         
        CKS2; CDC28 protein kinase regulatory subunit 2
        9 of 14
        116901
             
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Cks85A
            suppressible
            Cks30A
            (Ghorbani et al., 2011)
            Cks85A
            enhanceable
            Skp2
            (Ghorbani et al., 2011)
            Cks85A
            suppressible
            Skp2
            (Ghorbani et al., 2011)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Linkouts
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3R
            Recombination map
            3-48
            Cytogenetic map
            85A8-85A9
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            85A8-85A9
            Limits computationally determined from genome sequence between P{lacW}l(3)L4740L4740 and P{EP}D1EP473;
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            85A-85A
            (Swan, 2005.3.7)
            Experimentally Determined Recombination Data
            Location

            3-48

            (FlyBase, 2016)

            3-45.4

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (8)
            Genomic Clones (14)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (7)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments

                Cks85A functions to prevent polyploidy in mitotic cells.

                (Swan and Schupbach, 2005)
                Relationship to Other Genes
                Source for database merge of

                Source for merge of: CG9790 BcDNA:GM01344

                (FlyBase, 1996-)
                Additional comments

                Source for merge of CG9790 BcDNA:GM01344 was a shared cDNA ( date:030728 ).

                (FlyBase, 1996-)
                Nomenclature History
                Source for database identify of
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (6)
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 40 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                MIST (genetic) - An integrated Molecular Interaction Database
                MIST (protein-protein) - An integrated Molecular Interaction Database
                References (44)