Gene Dmel\CG9427

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General Information

Symbol

Dmel\CG9427

Species

D. melanogaster

Name

Annotation symbol

CG9427

Feature type

protein_coding_gene

FlyBase ID

FBgn0037721

Gene Model Status

Current

Stock availability

3 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

85E1-85E1

Sequence location

3R:5,450,879..5,452,652 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG9427 (FBgn0037721). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. The biological processes in which it is involved are not known. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Gamma interferon inducible lysosomal thiol reductase GILT; Thioredoxin-like fold. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed at stages throughout the larval period, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval/adult hindgut, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult crop, larval Malpighian tubules, larval trachea, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1627645_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG9427. Gene sequence location is 3R:5450879..5452652.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

85E1-85E1

Limits computationally determined from genome sequence between P{EP}CG9393^EP3122 and P{EP}Crc^EP3006

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG9427 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG9427-RA

FBtr0082104

NM_141662

847

213

CG9427-RB

FBtr0334619

NM_001275500

719

212

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG9427-PA

FBpp0081582

23.9

213

7.05

NP_649919

AAF54414

CG9427-PB

FBpp0306686

24.1

212

5.91

NP_001262429

AGB95811

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Gamma interferon inducible lysosomal thiol reductase GILT (IPR004911)

Thioredoxin-like fold (IPR012336)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VHA5

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

embryonic large intestine

(Fisher et al., 2012)

Additional Descriptive Data

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval/adult hindgut, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult crop, larval Malpighian tubules, larval trachea, larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		6.4
Larval Midgut		7.2
Larval Hindgut		601.2
Larval Malpighian Tubules		115.4
Larval Fat Body		3
Larval Salivary Gland		30.8
Larval Trachea		372.875
Larval Carcass		325.8
Adult Head		179.3
Adult Eye		750.85
Adult Brain		9.3
Adult Thoracic-Abdominal Ganglion		2.3
Adult Crop		204.7
Adult Midgut		3.5
Adult Hindgut		1073
Adult Malpighian Tubules		49.6
Adult Fat Body		3.1
Adult Salivary Gland		54
Adult Heart		1.075
Adult VirginFemale Spermatheca		15.5
Adult InseminatedFemale Spermatheca		16.5
Adult Ovary		4.8
Adult Testis		39
Adult Male Accessory Gland		2288.6
Adult Carcass		17.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		6.4
Larval Midgut		7.2
Larval Hindgut		(601.2)
Larval Malpighian Tubules		115.4
Larval Fat Body		3
Larval Salivary Gland		30.8
Larval Trachea		372.875
Larval Carcass		325.8
Adult Head		179.3
Adult Eye		(750.85)
Adult Brain		9.3
Adult Thoracic-Abdominal Ganglion		2.3
Adult Crop		204.7
Adult Midgut		3.5
Adult Hindgut		(1073)
Adult Malpighian Tubules		49.6
Adult Fat Body		3.1
Adult Salivary Gland		54
Adult Heart		1.075
Adult VirginFemale Spermatheca		15.5
Adult InseminatedFemale Spermatheca		16.5
Adult Ovary		4.8
Adult Testis		39
Adult Male Accessory Gland		(2288.6)
Adult Carcass		17.5
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		6.4
Larval Midgut		7.2
Larval Hindgut		601.2
Larval Malpighian Tubules		115.4
Larval Fat Body		3
Larval Salivary Gland		30.8
Larval Trachea		372.875
Larval Carcass		325.8
Adult Head		179.3
Adult Eye		750.85
Adult Brain		9.3
Adult Thoracic-Abdominal Ganglion		2.3
Adult Crop		204.7
Adult Midgut		3.5
Adult Hindgut		1073
Adult Malpighian Tubules		49.6
Adult Fat Body		3.1
Adult Salivary Gland		54
Adult Heart		1.075
Adult VirginFemale Spermatheca		15.5
Adult InseminatedFemale Spermatheca		16.5
Adult Ovary		4.8
Adult Testis		39
Adult Male Accessory Gland		(2288.6)
Adult Carcass		17.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		6.4
Larval Midgut		7.2
Larval Hindgut		601.2
Larval Malpighian Tubules		115.4
Larval Fat Body		3
Larval Salivary Gland		30.8
Larval Trachea		372.875
Larval Carcass		325.8
Adult Head		179.3
Adult Eye		750.85
Adult Brain		9.3
Adult Thoracic-Abdominal Ganglion		2.3
Adult Crop		204.7
Adult Midgut		3.5
Adult Hindgut		1073
Adult Malpighian Tubules		49.6
Adult Fat Body		3.1
Adult Salivary Gland		54
Adult Heart		1.075
Adult VirginFemale Spermatheca		15.5
Adult InseminatedFemale Spermatheca		16.5
Adult Ovary		4.8
Adult Testis		39
Adult Male Accessory Gland		2288.6
Adult Carcass		17.5
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		6.4
Larval Midgut		7.2
Larval Hindgut		601.2
Larval Malpighian Tubules		115.4
Larval Fat Body		3
Larval Salivary Gland		30.8
Larval Trachea		372.875
Larval Carcass		325.8
Adult Head		179.3
Adult Eye		750.85
Adult Brain		9.3
Adult Thoracic-Abdominal Ganglion		2.3
Adult Crop		204.7
Adult Midgut		3.5
Adult Hindgut		1073
Adult Malpighian Tubules		49.6
Adult Fat Body		3.1
Adult Salivary Gland		54
Adult Heart		1.075
Adult VirginFemale Spermatheca		15.5
Adult InseminatedFemale Spermatheca		16.5
Adult Ovary		4.8
Adult Testis		39
Adult Male Accessory Gland		2288.6
Adult Carcass		17.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		6.4
Larval Midgut		7.2
Larval Hindgut		601.2
Larval Malpighian Tubules		115.4
Larval Fat Body		3
Larval Salivary Gland		30.8
Larval Trachea		372.875
Larval Carcass		325.8
Adult Head		179.3
Adult Eye		750.85
Adult Brain		9.3
Adult Thoracic-Abdominal Ganglion		2.3
Adult Crop		204.7
Adult Midgut		3.5
Adult Hindgut		(1073)
Adult Malpighian Tubules		49.6
Adult Fat Body		3.1
Adult Salivary Gland		54
Adult Heart		1.075
Adult VirginFemale Spermatheca		15.5
Adult InseminatedFemale Spermatheca		16.5
Adult Ovary		4.8
Adult Testis		39
Adult Male Accessory Gland		(2288.6)
Adult Carcass		17.5
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		6.4
Larval Midgut		7.2
Larval Hindgut		601.2
Larval Malpighian Tubules		115.4
Larval Fat Body		3
Larval Salivary Gland		30.8
Larval Trachea		372.875
Larval Carcass		325.8
Adult Head		179.3
Adult Eye		750.85
Adult Brain		9.3
Adult Thoracic-Abdominal Ganglion		2.3
Adult Crop		204.7
Adult Midgut		3.5
Adult Hindgut		1073
Adult Malpighian Tubules		49.6
Adult Fat Body		3.1
Adult Salivary Gland		54
Adult Heart		1.075
Adult VirginFemale Spermatheca		15.5
Adult InseminatedFemale Spermatheca		16.5
Adult Ovary		4.8
Adult Testis		39
Adult Male Accessory Gland		(2288.6)
Adult Carcass		17.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		6.4
Larval Midgut		7.2
Larval Hindgut		601.2
Larval Malpighian Tubules		115.4
Larval Fat Body		3
Larval Salivary Gland		30.8
Larval Trachea		372.875
Larval Carcass		325.8
Adult Head		179.3
Adult Eye		750.85
Adult Brain		9.3
Adult Thoracic-Abdominal Ganglion		2.3
Adult Crop		204.7
Adult Midgut		3.5
Adult Hindgut		1073
Adult Malpighian Tubules		49.6
Adult Fat Body		3.1
Adult Salivary Gland		54
Adult Heart		1.075
Adult VirginFemale Spermatheca		15.5
Adult InseminatedFemale Spermatheca		16.5
Adult Ovary		4.8
Adult Testis		39
Adult Male Accessory Gland		2288.6
Adult Carcass		17.5
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	179.3
NA	Eye	750.85
NA	Brain	9.3
6.4	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	2.3
NA	Crop	204.7
7.2	Midgut	3.5
601.2	Hindgut	1073
115.4	Malpighian Tubules	no informative data
3	Fat Body	3.1
no informative data	Salivary Gland	no informative data
NA	Heart	1.075
372.875	Trachea	NA
NA	VirginFemale Spermatheca	no informative data
NA	InseminatedFemale Spermatheca	no informative data
NA	Ovary	4.8
NA	Testis	39
NA	Male Accessory Gland	2288.6
325.8	Carcass	no informative data

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		16
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		12
head, virgin 4-day female		10
head, virgin 20-day female		8
head, mated 1-day female		10
head, mated 4-day female		8
head, mated 20-day female		7
head, mated 1-day male		20
head, mated 4-day male		11
head, mated 20-day male		14
salivary gland, larvae L3 wandering		71
salivary gland, white prepupae		709
digestive system, larvae L3 wandering		10
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		0
fat body, white prepupae		5
fat body, pupae P8		4
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		10
accessory gland, mated 4-day male		61
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		16
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		12
head, virgin 4-day female		10
head, virgin 20-day female		8
head, mated 1-day female		10
head, mated 4-day female		8
head, mated 20-day female		7
head, mated 1-day male		20
head, mated 4-day male		11
head, mated 20-day male		14
salivary gland, larvae L3 wandering		(71)
salivary gland, white prepupae		(709)
digestive system, larvae L3 wandering		10
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		0
fat body, white prepupae		5
fat body, pupae P8		4
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		10
accessory gland, mated 4-day male		(61)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		16
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		12
head, virgin 4-day female		10
head, virgin 20-day female		8
head, mated 1-day female		10
head, mated 4-day female		8
head, mated 20-day female		7
head, mated 1-day male		20
head, mated 4-day male		11
head, mated 20-day male		14
salivary gland, larvae L3 wandering		71
salivary gland, white prepupae		(709)
digestive system, larvae L3 wandering		10
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		0
fat body, white prepupae		5
fat body, pupae P8		4
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		10
accessory gland, mated 4-day male		61
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		16
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		12
head, virgin 4-day female		10
head, virgin 20-day female		8
head, mated 1-day female		10
head, mated 4-day female		8
head, mated 20-day female		7
head, mated 1-day male		20
head, mated 4-day male		11
head, mated 20-day male		14
salivary gland, larvae L3 wandering		71
salivary gland, white prepupae		709
digestive system, larvae L3 wandering		10
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		0
fat body, white prepupae		5
fat body, pupae P8		4
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		10
accessory gland, mated 4-day male		61
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		16
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		12
head, virgin 4-day female		10
head, virgin 20-day female		8
head, mated 1-day female		10
head, mated 4-day female		8
head, mated 20-day female		7
head, mated 1-day male		20
head, mated 4-day male		11
head, mated 20-day male		14
salivary gland, larvae L3 wandering		71
salivary gland, white prepupae		709
digestive system, larvae L3 wandering		10
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		0
fat body, white prepupae		5
fat body, pupae P8		4
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		10
accessory gland, mated 4-day male		61
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		16
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		12
head, virgin 4-day female		10
head, virgin 20-day female		8
head, mated 1-day female		10
head, mated 4-day female		8
head, mated 20-day female		7
head, mated 1-day male		20
head, mated 4-day male		11
head, mated 20-day male		14
salivary gland, larvae L3 wandering		(71)
salivary gland, white prepupae		(709)
digestive system, larvae L3 wandering		10
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		0
fat body, white prepupae		5
fat body, pupae P8		4
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		10
accessory gland, mated 4-day male		(61)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		16
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		12
head, virgin 4-day female		10
head, virgin 20-day female		8
head, mated 1-day female		10
head, mated 4-day female		8
head, mated 20-day female		7
head, mated 1-day male		20
head, mated 4-day male		11
head, mated 20-day male		14
salivary gland, larvae L3 wandering		71
salivary gland, white prepupae		(709)
digestive system, larvae L3 wandering		10
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		0
fat body, white prepupae		5
fat body, pupae P8		4
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		10
accessory gland, mated 4-day male		61
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		16
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		12
head, virgin 4-day female		10
head, virgin 20-day female		8
head, mated 1-day female		10
head, mated 4-day female		8
head, mated 20-day female		7
head, mated 1-day male		20
head, mated 4-day male		11
head, mated 20-day male		14
salivary gland, larvae L3 wandering		71
salivary gland, white prepupae		709
digestive system, larvae L3 wandering		10
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		0
fat body, white prepupae		5
fat body, pupae P8		4
carcass, larvae L3 wandering		13
carcass, 1-day adult		2
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		10
accessory gland, mated 4-day male		61
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed at stages throughout the larval period, at stages throughout the pupal period.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		7
embryo 12-14hr		7
embryo 14-16hr		11
embryo 16-18hr		15
embryo 18-20hr		20
embryo 20-22hr		20
embryo 22-24hr		23
larva L1		38
larva L2		54
larva L3 12hr old		38
larva L3 puffstage 1-2		24
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		55
white prepupae new		96
white prepupae 12hr		67
white prepupae 24hr		31
pupae 2d postWPP		20
pupae 3d postWPP		90
pupae 4d postWPP		21
adult male 01day		35
adult male 05day		34
adult male 30day		35
adult female 01day		14
adult female 05day		6
adult female 30day		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		7
embryo 12-14hr		7
embryo 14-16hr		11
embryo 16-18hr		15
embryo 18-20hr		20
embryo 20-22hr		20
embryo 22-24hr		23
larva L1		(38)
larva L2		(54)
larva L3 12hr old		(38)
larva L3 puffstage 1-2		24
larva L3 puffstage 3-6		(27)
larva L3 puffstage 7-9		(55)
white prepupae new		(96)
white prepupae 12hr		(67)
white prepupae 24hr		(31)
pupae 2d postWPP		20
pupae 3d postWPP		(90)
pupae 4d postWPP		21
adult male 01day		(35)
adult male 05day		(34)
adult male 30day		(35)
adult female 01day		14
adult female 05day		6
adult female 30day		5
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		7
embryo 12-14hr		7
embryo 14-16hr		11
embryo 16-18hr		15
embryo 18-20hr		20
embryo 20-22hr		20
embryo 22-24hr		23
larva L1		38
larva L2		54
larva L3 12hr old		38
larva L3 puffstage 1-2		24
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		55
white prepupae new		96
white prepupae 12hr		67
white prepupae 24hr		31
pupae 2d postWPP		20
pupae 3d postWPP		90
pupae 4d postWPP		21
adult male 01day		35
adult male 05day		34
adult male 30day		35
adult female 01day		14
adult female 05day		6
adult female 30day		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		7
embryo 12-14hr		7
embryo 14-16hr		11
embryo 16-18hr		15
embryo 18-20hr		20
embryo 20-22hr		20
embryo 22-24hr		23
larva L1		38
larva L2		54
larva L3 12hr old		38
larva L3 puffstage 1-2		24
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		55
white prepupae new		96
white prepupae 12hr		67
white prepupae 24hr		31
pupae 2d postWPP		20
pupae 3d postWPP		90
pupae 4d postWPP		21
adult male 01day		35
adult male 05day		34
adult male 30day		35
adult female 01day		14
adult female 05day		6
adult female 30day		5
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		7
embryo 12-14hr		7
embryo 14-16hr		11
embryo 16-18hr		15
embryo 18-20hr		20
embryo 20-22hr		20
embryo 22-24hr		23
larva L1		38
larva L2		54
larva L3 12hr old		38
larva L3 puffstage 1-2		24
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		55
white prepupae new		96
white prepupae 12hr		67
white prepupae 24hr		31
pupae 2d postWPP		20
pupae 3d postWPP		90
pupae 4d postWPP		21
adult male 01day		35
adult male 05day		34
adult male 30day		35
adult female 01day		14
adult female 05day		6
adult female 30day		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		7
embryo 12-14hr		7
embryo 14-16hr		11
embryo 16-18hr		15
embryo 18-20hr		20
embryo 20-22hr		20
embryo 22-24hr		23
larva L1		(38)
larva L2		(54)
larva L3 12hr old		(38)
larva L3 puffstage 1-2		24
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		(55)
white prepupae new		(96)
white prepupae 12hr		(67)
white prepupae 24hr		31
pupae 2d postWPP		20
pupae 3d postWPP		(90)
pupae 4d postWPP		21
adult male 01day		(35)
adult male 05day		(34)
adult male 30day		(35)
adult female 01day		14
adult female 05day		6
adult female 30day		5
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		7
embryo 12-14hr		7
embryo 14-16hr		11
embryo 16-18hr		15
embryo 18-20hr		20
embryo 20-22hr		20
embryo 22-24hr		23
larva L1		38
larva L2		54
larva L3 12hr old		38
larva L3 puffstage 1-2		24
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		55
white prepupae new		96
white prepupae 12hr		67
white prepupae 24hr		31
pupae 2d postWPP		20
pupae 3d postWPP		90
pupae 4d postWPP		21
adult male 01day		35
adult male 05day		34
adult male 30day		35
adult female 01day		14
adult female 05day		6
adult female 30day		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		7
embryo 12-14hr		7
embryo 14-16hr		11
embryo 16-18hr		15
embryo 18-20hr		20
embryo 20-22hr		20
embryo 22-24hr		23
larva L1		38
larva L2		54
larva L3 12hr old		38
larva L3 puffstage 1-2		24
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		55
white prepupae new		96
white prepupae 12hr		67
white prepupae 24hr		31
pupae 2d postWPP		20
pupae 3d postWPP		90
pupae 4d postWPP		21
adult male 01day		35
adult male 05day		34
adult male 30day		35
adult female 01day		14
adult female 05day		6
adult female 30day		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		1
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		2
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		1
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		2
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		1
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		2
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		1
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		2
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		1
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		2
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		1
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		2
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		1
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		2
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		1
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		2
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		27
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		16
Cadmium 50 mM 12 hrs, larvae L3		23
Cadmium 50 mM 48 hrs, 4-day adult		9
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		12
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		4
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		16
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(33)
cold shock, 4-day adult		(27)
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		16
Cadmium 50 mM 12 hrs, larvae L3		23
Cadmium 50 mM 48 hrs, 4-day adult		9
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		12
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		4
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		16
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		27
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		16
Cadmium 50 mM 12 hrs, larvae L3		23
Cadmium 50 mM 48 hrs, 4-day adult		9
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		12
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		4
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		16
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		27
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		16
Cadmium 50 mM 12 hrs, larvae L3		23
Cadmium 50 mM 48 hrs, 4-day adult		9
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		12
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		4
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		16
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		27
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		16
Cadmium 50 mM 12 hrs, larvae L3		23
Cadmium 50 mM 48 hrs, 4-day adult		9
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		12
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		4
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		16
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(33)
cold shock, 4-day adult		27
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		16
Cadmium 50 mM 12 hrs, larvae L3		23
Cadmium 50 mM 48 hrs, 4-day adult		9
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		12
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		4
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		16
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		27
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		16
Cadmium 50 mM 12 hrs, larvae L3		23
Cadmium 50 mM 48 hrs, 4-day adult		9
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		12
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		4
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		16
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		33
cold shock, 4-day adult		27
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		16
Cadmium 50 mM 12 hrs, larvae L3		23
Cadmium 50 mM 48 hrs, 4-day adult		9
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		12
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		4
Ethanol 5% 3 hrs, larvae L3		11
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		16
Rotenone 8 μg 12 hrs, larvae L3		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_18

External Data & Images

Linkouts

BDGP expression data

•

GH19763

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0037721

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG9427-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for CG9427

Stages(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | pupal stage, with Scer\GAL4^pnr-MD237

CG9427^GD4787

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of CG9427	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG9427	Mutagen	Stocks	Known lesion
CG9427^GD4787	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
CG9427^KK110095	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG9427^NIG.9427R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3R)Exel6153

(Ryder, 2004.4.26)

Df(3R)Exel6264

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4787}

P{KK110095}

P{NIG.9427R}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 0 terms )

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

CG9427 - CG7049

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

CG9427 - Cg25C

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG9427 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

66323

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0037721

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0037721

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

41164

Orthologs

OrthoDB Orthologs (20) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6V17M5

Dipteran inclusive ortholog search

EOG61NTW3

Insect inclusive ortholog search

EOG6PG4XQ

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG6V17M5)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21779

Drosophila persimilis

Dper\GL22172

Drosophila willistoni

Dwil\GK11854

Drosophila virilis

Dvir\GJ24579

Drosophila mojavensis

Dmoj\GI23067

Drosophila grimshawi

Dgri\GH19011

Orthologs in non-Drosophila Dipterans (EOG61NTW3)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Anopheles gambiae

Malaria mosquito

Agam\AGAP002989

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002338

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002339

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ007525

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ007524

Orthologs in non-Dipteran Insects (EOG6PG4XQ)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004563

Bombyx mori

Silkmoth

Bmor\BGIBMGA008360

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07331

Orthologs in non-Insect Arthropods (None identified)

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21779

Drosophila persimilis

Dper\GL22172

Drosophila willistoni

Dwil\GK11854

Drosophila virilis

Dvir\GJ24579

Drosophila mojavensis

Dmoj\GI23067

Drosophila grimshawi

Dgri\GH19011

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v106360

P{KK110095}VIE-260B

v15375

w¹¹¹⁸; P{GD4787}v15375

v15374

w¹¹¹⁸; P{GD4787}v15374/CyO

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 11 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH19763

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0037721

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

41164

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

41164

UniProtKB/TrEMBL

•

Q9VHA5

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Gamma interferon inducible lysosomal thiol reductase GILT (IPR004911)

Thioredoxin-like fold (IPR012336)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

GH19763

Linkouts

BioGRID - A database of protein and genetic interactions

•

66323

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0037721

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0037721

DRSC - Results from RNAi screens.

•

FBgn0037721

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0037721

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG9427-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0037721

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

41164

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

41164

modMine - Data generated by the modENCODE project.

•

FBgn0037721

Synonyms & Secondary IDs ( 1 )

Reported As

Symbol Synonym

CG9427

(Wijnen et al., 2006, Keleman et al., 2009.8.5, Japanese National Institute of Genetics, 2012.5.21)

Name Synonym

Secondary FlyBase IDs

References ( 16 )

Research paper

Wijnen et al., 2006, PLoS Genet. 2(3): e39: Control of daily transcript oscillations in Drosophila by light and the circadian clock. [FBrf0191393]
Klebes et al., 2005, Development 132(16): 3753--3765: Regulation of cellular plasticity in Drosophila imaginal disc cells by the Polycomb group, trithorax group and lama genes. [FBrf0187468]
Xu et al., 2004, EMBO J. 23(4): 811--822: A lysosomal tetraspanin associated with retinal degeneration identified via a genome-wide screen. [FBrf0174603]
Dimova et al., 2003, Genes Dev. 17(18): 2308--2320: Cell cycle-dependent and cell cycle-independent control of transcription by the Drosophila E2F/RB pathway. [FBrf0162136]
Michaut et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100(7): 4024--4029: Analysis of the eye developmental pathway in Drosophila using DNA microarrays. [FBrf0159295]

Supplementary material

The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project: DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

Stock list

Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.: RNAi stocks available from NIG-FLY. [FBrf0218457]

Website

Fisher et al., 2012, BDGP insitu homepage: BDGP insitu homepage [FBrf0219073]