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Gene Dmel\Bruce

General Information
Symbol	Dmel\Bruce	Species	D. melanogaster
Name	Bruce	Annotation symbol	CG6303
Feature type	protein_coding_gene	FlyBase ID	FBgn0037808
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	dBruce
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	86A7-86A8	Sequence location	3R:6,137,757..6,157,075 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Bruce is referred to in FlyBase by the symbol Dmel\Bruce (CG6303, FBgn0037808). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ubiquitin-protein ligase activity. There is experimental evidence that it is involved in the biological process: spermatid development; negative regulation of apoptotic process; programmed cell death. 22 alleles are reported. The phenotype of these alleles is annotated with: egg chamber. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Baculoviral inhibition of apoptosis protein repeat; Protein of unknown function DUF3643; Ubiquitin-conjugating enzyme, E2; Ubiquitin-conjugating enzyme/RWD-like; WD40-repeat-containing domain; WD40/YVTN repeat-like-containing domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1634454_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Bruce. Gene sequence location is 3R:6137757..6157075.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 86A7-86A8   Limits computationally determined from genome sequence between P{EP}Fmr1EP3517&P{PZ}tws02414 and P{PZ}jumu06439&P{lacW}l(3)j8B6j8B6
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Bruce for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Bruce-RA FBtr0082239  NM_141738   15406   4876 Bruce-RB FBtr0331681  NM_001275531   15706   4976 Bruce-RC FBtr0331682  NM_001275532   15334   4852 Bruce-RD FBtr0331683  NM_001275533   15403   4875 Bruce-RE FBtr0331684  NM_001275534   15373   4865
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Bruce-PA   FBpp0081717   539.2   4876   5.26     NP_649995   AAF54520 Bruce-PB   FBpp0304070   550.3   4976   5.44     NP_001262460   AGB95842 Bruce-PC   FBpp0304071   536.5   4852   5.29     NP_001262461   AGB95843 Bruce-PD   FBpp0304072   539.1   4875   5.26     NP_001262462   AGB95844 Bruce-PE   FBpp0304073   537.9   4865   5.26     NP_001262463   AGB95845
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • WD40-repeat-containing domain (IPR017986) Ubiquitin-conjugating enzyme, E2 (IPR000608) Baculoviral inhibition of apoptosis protein repeat (IPR001370) Ubiquitin-conjugating enzyme/RWD-like (IPR016135) Protein of unknown function DUF3643 (IPR022103) WD40/YVTN repeat-like-containing domain (IPR015943)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA978573   AE014297 AAF54520   AF517634 AAM77747   AE014297 AGB95842   AE014297 AGB95843   AE014297 AGB95844   AE014297 AGB95845   AI113838   AI257027   AY119104 AAM50964   BF499746   BI167572   BI352722   BI353051   BI564273   BI586947   BI620316   BI638161   BI640753   BP547800   BT132668   BT132706   CK135263   CK604609   CK658391   CO155303   CO176986   CO186893   CO261889   CO263874   CO270294   CO284718   CO291274   CO292880   CO293196   CO303497   CO317200   CO327620   CO340060   CO340142   CZ478812   CZ478813   EC054854   EC059316   EC063720   EC066593   EC072294   EC081056   EC081900   EC088595   EC212477   EC220147   EC223791   EC230124   EC242436   EC252034   EC261082   EV579949   GH747666   GH747923   GH748006   GH750626   GH751710   GH753310   GH754408   GH757881   GH758146   GH759068   GH760520   GH760544   GH770849   GH772912   GH777334   GH777558   GH778152   GH781048   GH783549   GH787899   GH789611   GH790410   GH791966   GH792438   GH793617   GH793955   GH794545   GH802723   GH803721   GH806030   GH806924   GH807037   GH808726   GH808960   GH812439   GH812835   GH815727   GH816149   GH816686   GH817343   GH817705   GH820632   GH821634   GH822203   GH824277   GH825899   GH826422   GH828632   GH828980   GH831850   GH832255   GH834708   GH836871   GH837782   GH838487   GH840446   GH840592   GH840839   GH841398   GH841672   GH841925   GH842522   GH842620   GH843648   GH846056   GH848785   GH849179   GH849950   GH851391   GH851411   GH854912   GH857190   GH857629   GH857913   GH858912   GH859471   GH860156   GH861519   GH861783   GH862004   GH862074   GH864357   GH865078   GH868963   GH870977   GH871476   GH871779   GH871820   GH872442   GH872577   GH873524   GH873970   GH874098   GH874745   GH875546   GH878086   GH878370   GH878528   GH878646   GH880839   GH885667   GH885970   GH886755   GH886837   GH887447   GH888231   GH888299   GH888914   GH889614   GH889693   GH889744   GH894714   GH897503   GH897996   GH899237   GH900036   GH900766   GH900870   GH901756   GH903587   GH905767   GH908529   GH910198   GH911460   GH912590   GH914147   GH914189   GH918326   GH918497   GH919930   GH920364   GH920596   GH921088   GH922092   GH922336   GH923708   GH924282   GH924939   GH925795   GH927915   GH927944   GH928562   GH928613   GH931260   GH931932   GH932882   GH933426   GH933850   GH936759   GH938980   GH940451
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VH01 Q8MS47
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    144.85 Larval Midgut    92.2 Larval Hindgut    135.4 Larval Malpighian Tubules    174.3 Larval Fat Body    103.7 Larval Salivary Gland    231.8 Larval Trachea    129.3 Larval Carcass    108.175 Adult Head    144.5 Adult Eye    93.25 Adult Brain    190.7 Adult Thoracic-Abdominal Ganglion    150.8 Adult Crop    181.6 Adult Midgut    123.8 Adult Hindgut    188.9 Adult Malpighian Tubules    160.3 Adult Fat Body    136.2 Adult Salivary Gland    151.3 Adult Heart    124.925 Adult VirginFemale Spermatheca    65.6 Adult InseminatedFemale Spermatheca    64.5 Adult Ovary    187.2 Adult Testis    224.3 Adult Male Accessory Gland    238.2 Adult Carcass    182.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    144.85 Larval Midgut    92.2 Larval Hindgut    135.4 Larval Malpighian Tubules    174.3 Larval Fat Body    103.7 Larval Salivary Gland    231.8 Larval Trachea    129.3 Larval Carcass    108.175 Adult Head    144.5 Adult Eye    93.25 Adult Brain    190.7 Adult Thoracic-Abdominal Ganglion    150.8 Adult Crop    181.6 Adult Midgut    123.8 Adult Hindgut    188.9 Adult Malpighian Tubules    160.3 Adult Fat Body    136.2 Adult Salivary Gland    151.3 Adult Heart    124.925 Adult VirginFemale Spermatheca    65.6 Adult InseminatedFemale Spermatheca    64.5 Adult Ovary    187.2 Adult Testis    224.3 Adult Male Accessory Gland    238.2 Adult Carcass    182.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    144.85 Larval Midgut    92.2 Larval Hindgut    135.4 Larval Malpighian Tubules    174.3 Larval Fat Body    103.7 Larval Salivary Gland    231.8 Larval Trachea    129.3 Larval Carcass    108.175 Adult Head    144.5 Adult Eye    93.25 Adult Brain    190.7 Adult Thoracic-Abdominal Ganglion    150.8 Adult Crop    181.6 Adult Midgut    123.8 Adult Hindgut    188.9 Adult Malpighian Tubules    160.3 Adult Fat Body    136.2 Adult Salivary Gland    151.3 Adult Heart    124.925 Adult VirginFemale Spermatheca    65.6 Adult InseminatedFemale Spermatheca    64.5 Adult Ovary    187.2 Adult Testis    224.3 Adult Male Accessory Gland    238.2 Adult Carcass    182.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    144.85 Larval Midgut    92.2 Larval Hindgut    135.4 Larval Malpighian Tubules    174.3 Larval Fat Body    103.7 Larval Salivary Gland    231.8 Larval Trachea    129.3 Larval Carcass    108.175 Adult Head    144.5 Adult Eye    93.25 Adult Brain    190.7 Adult Thoracic-Abdominal Ganglion    150.8 Adult Crop    181.6 Adult Midgut    123.8 Adult Hindgut    188.9 Adult Malpighian Tubules    160.3 Adult Fat Body    136.2 Adult Salivary Gland    151.3 Adult Heart    124.925 Adult VirginFemale Spermatheca    65.6 Adult InseminatedFemale Spermatheca    64.5 Adult Ovary    187.2 Adult Testis    224.3 Adult Male Accessory Gland    238.2 Adult Carcass    182.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    144.85 Larval Midgut    92.2 Larval Hindgut    135.4 Larval Malpighian Tubules    174.3 Larval Fat Body    103.7 Larval Salivary Gland    231.8 Larval Trachea    129.3 Larval Carcass    108.175 Adult Head    144.5 Adult Eye    93.25 Adult Brain    190.7 Adult Thoracic-Abdominal Ganglion    150.8 Adult Crop    181.6 Adult Midgut    123.8 Adult Hindgut    188.9 Adult Malpighian Tubules    160.3 Adult Fat Body    136.2 Adult Salivary Gland    151.3 Adult Heart    124.925 Adult VirginFemale Spermatheca    65.6 Adult InseminatedFemale Spermatheca    64.5 Adult Ovary    187.2 Adult Testis    224.3 Adult Male Accessory Gland    238.2 Adult Carcass    182.7 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    144.85 Larval Midgut    92.2 Larval Hindgut    135.4 Larval Malpighian Tubules    174.3 Larval Fat Body    103.7 Larval Salivary Gland    231.8 Larval Trachea    129.3 Larval Carcass    108.175 Adult Head    144.5 Adult Eye    93.25 Adult Brain    190.7 Adult Thoracic-Abdominal Ganglion    150.8 Adult Crop    181.6 Adult Midgut    123.8 Adult Hindgut    188.9 Adult Malpighian Tubules    160.3 Adult Fat Body    136.2 Adult Salivary Gland    151.3 Adult Heart    124.925 Adult VirginFemale Spermatheca    65.6 Adult InseminatedFemale Spermatheca    64.5 Adult Ovary    187.2 Adult Testis    224.3 Adult Male Accessory Gland    238.2 Adult Carcass    182.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    144.85 Larval Midgut    92.2 Larval Hindgut    135.4 Larval Malpighian Tubules    174.3 Larval Fat Body    103.7 Larval Salivary Gland    231.8 Larval Trachea    129.3 Larval Carcass    108.175 Adult Head    144.5 Adult Eye    93.25 Adult Brain    190.7 Adult Thoracic-Abdominal Ganglion    150.8 Adult Crop    181.6 Adult Midgut    123.8 Adult Hindgut    188.9 Adult Malpighian Tubules    160.3 Adult Fat Body    136.2 Adult Salivary Gland    151.3 Adult Heart    124.925 Adult VirginFemale Spermatheca    65.6 Adult InseminatedFemale Spermatheca    64.5 Adult Ovary    187.2 Adult Testis    224.3 Adult Male Accessory Gland    238.2 Adult Carcass    182.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    144.85 Larval Midgut    92.2 Larval Hindgut    135.4 Larval Malpighian Tubules    174.3 Larval Fat Body    103.7 Larval Salivary Gland    231.8 Larval Trachea    129.3 Larval Carcass    108.175 Adult Head    144.5 Adult Eye    93.25 Adult Brain    190.7 Adult Thoracic-Abdominal Ganglion    150.8 Adult Crop    181.6 Adult Midgut    123.8 Adult Hindgut    188.9 Adult Malpighian Tubules    160.3 Adult Fat Body    136.2 Adult Salivary Gland    151.3 Adult Heart    124.925 Adult VirginFemale Spermatheca    65.6 Adult InseminatedFemale Spermatheca    64.5 Adult Ovary    187.2 Adult Testis    224.3 Adult Male Accessory Gland    238.2 Adult Carcass    182.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    144.5   NA  Eye    93.25   NA  Brain    190.7   144.85  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    150.8   NA  Crop    181.6   92.2  Midgut    123.8   135.4  Hindgut    188.9   174.3  Malpighian Tubules    160.3   103.7  Fat Body    136.2   231.8  Salivary Gland    151.3   NA  Heart    124.925   129.3  Trachea    NA   NA  VirginFemale Spermatheca    65.6   NA  InseminatedFemale Spermatheca    64.5   NA  Ovary    187.2   NA  Testis    224.3   NA  Male Accessory Gland    238.2   108.175  Carcass    182.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    12 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    18 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    8 fat body, larvae L3 wandering    6 fat body, white prepupae    9 fat body, pupae P8    8 carcass, larvae L3 wandering    16 carcass, 1-day adult    9 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    20 ovary, mated 4-day female    20 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    12 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    18 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    8 fat body, larvae L3 wandering    6 fat body, white prepupae    9 fat body, pupae P8    8 carcass, larvae L3 wandering    16 carcass, 1-day adult    9 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    20 ovary, mated 4-day female    20 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    12 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    18 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    8 fat body, larvae L3 wandering    6 fat body, white prepupae    9 fat body, pupae P8    8 carcass, larvae L3 wandering    16 carcass, 1-day adult    9 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    20 ovary, mated 4-day female    20 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    12 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    18 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    8 fat body, larvae L3 wandering    6 fat body, white prepupae    9 fat body, pupae P8    8 carcass, larvae L3 wandering    16 carcass, 1-day adult    9 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    20 ovary, mated 4-day female    20 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    12 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    18 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    8 fat body, larvae L3 wandering    6 fat body, white prepupae    9 fat body, pupae P8    8 carcass, larvae L3 wandering    16 carcass, 1-day adult    9 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    20 ovary, mated 4-day female    20 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    12 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    18 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    8 fat body, larvae L3 wandering    6 fat body, white prepupae    9 fat body, pupae P8    8 carcass, larvae L3 wandering    16 carcass, 1-day adult    9 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    20 ovary, mated 4-day female    20 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    12 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    18 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    8 fat body, larvae L3 wandering    6 fat body, white prepupae    9 fat body, pupae P8    8 carcass, larvae L3 wandering    16 carcass, 1-day adult    9 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    20 ovary, mated 4-day female    20 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    20 central nervous system, pupae P8    20 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    12 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    18 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    8 fat body, larvae L3 wandering    6 fat body, white prepupae    9 fat body, pupae P8    8 carcass, larvae L3 wandering    16 carcass, 1-day adult    9 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    20 ovary, mated 4-day female    20 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    8 embryo 04-06hr    17 embryo 06-08hr    21 embryo 08-10hr    17 embryo 10-12hr    8 embryo 12-14hr    16 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    12 larva L1    13 larva L2    10 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    18 white prepupae new    18 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    19 pupae 3d postWPP    13 pupae 4d postWPP    13 adult male 01day    21 adult male 05day    23 adult male 30day    22 adult female 01day    16 adult female 05day    22 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    8 embryo 04-06hr    17 embryo 06-08hr    21 embryo 08-10hr    17 embryo 10-12hr    8 embryo 12-14hr    16 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    12 larva L1    13 larva L2    10 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    18 white prepupae new    18 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    19 pupae 3d postWPP    13 pupae 4d postWPP    13 adult male 01day    21 adult male 05day    23 adult male 30day    22 adult female 01day    16 adult female 05day    22 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    8 embryo 04-06hr    17 embryo 06-08hr    21 embryo 08-10hr    17 embryo 10-12hr    8 embryo 12-14hr    16 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    12 larva L1    13 larva L2    10 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    18 white prepupae new    18 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    19 pupae 3d postWPP    13 pupae 4d postWPP    13 adult male 01day    21 adult male 05day    23 adult male 30day    22 adult female 01day    16 adult female 05day    22 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    8 embryo 04-06hr    17 embryo 06-08hr    21 embryo 08-10hr    17 embryo 10-12hr    8 embryo 12-14hr    16 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    12 larva L1    13 larva L2    10 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    18 white prepupae new    18 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    19 pupae 3d postWPP    13 pupae 4d postWPP    13 adult male 01day    21 adult male 05day    23 adult male 30day    22 adult female 01day    16 adult female 05day    22 adult female 30day    21 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    8 embryo 04-06hr    17 embryo 06-08hr    21 embryo 08-10hr    17 embryo 10-12hr    8 embryo 12-14hr    16 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    12 larva L1    13 larva L2    10 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    18 white prepupae new    18 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    19 pupae 3d postWPP    13 pupae 4d postWPP    13 adult male 01day    21 adult male 05day    23 adult male 30day    22 adult female 01day    16 adult female 05day    22 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    8 embryo 04-06hr    17 embryo 06-08hr    21 embryo 08-10hr    17 embryo 10-12hr    8 embryo 12-14hr    16 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    12 larva L1    13 larva L2    10 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    18 white prepupae new    18 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    19 pupae 3d postWPP    13 pupae 4d postWPP    13 adult male 01day    21 adult male 05day    23 adult male 30day    22 adult female 01day    16 adult female 05day    22 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    8 embryo 04-06hr    17 embryo 06-08hr    21 embryo 08-10hr    17 embryo 10-12hr    8 embryo 12-14hr    16 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    12 larva L1    13 larva L2    10 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    18 white prepupae new    18 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    19 pupae 3d postWPP    13 pupae 4d postWPP    13 adult male 01day    21 adult male 05day    23 adult male 30day    22 adult female 01day    16 adult female 05day    22 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    8 embryo 04-06hr    17 embryo 06-08hr    21 embryo 08-10hr    17 embryo 10-12hr    8 embryo 12-14hr    16 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    12 larva L1    13 larva L2    10 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    18 white prepupae new    18 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    19 pupae 3d postWPP    13 pupae 4d postWPP    13 adult male 01day    21 adult male 05day    23 adult male 30day    22 adult female 01day    16 adult female 05day    22 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    7 embryonic 1182-4H    24 embryonic GM2    37 embryonic Kc167    17 embryonic S1    26 embryonic S3    22 leg disc CME L1    10 wing disc CME-W2    17 wing disc ML-DmD8    15 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    19 ovary fGS/OSS    34 ovary OSC    33 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    7 embryonic 1182-4H    24 embryonic GM2  (37) embryonic Kc167    17 embryonic S1  26 embryonic S3    22 leg disc CME L1    10 wing disc CME-W2    17 wing disc ML-DmD8    15 wing disc ML-DmD9    18 wing disc ML-DmD16-c3  (31) wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11  (54) antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    19 ovary fGS/OSS  (34) ovary OSC  (33) ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    7 embryonic 1182-4H    24 embryonic GM2    37 embryonic Kc167    17 embryonic S1    26 embryonic S3    22 leg disc CME L1    10 wing disc CME-W2    17 wing disc ML-DmD8    15 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    19 ovary fGS/OSS    34 ovary OSC    33 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    7 embryonic 1182-4H    24 embryonic GM2    37 embryonic Kc167    17 embryonic S1    26 embryonic S3    22 leg disc CME L1    10 wing disc CME-W2    17 wing disc ML-DmD8    15 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    19 ovary fGS/OSS    34 ovary OSC    33 ovary OSS    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    7 embryonic 1182-4H    24 embryonic GM2    37 embryonic Kc167    17 embryonic S1    26 embryonic S3    22 leg disc CME L1    10 wing disc CME-W2    17 wing disc ML-DmD8    15 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    19 ovary fGS/OSS    34 ovary OSC    33 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    7 embryonic 1182-4H    24 embryonic GM2  (37) embryonic Kc167    17 embryonic S1  26 embryonic S3    22 leg disc CME L1    10 wing disc CME-W2    17 wing disc ML-DmD8    15 wing disc ML-DmD9    18 wing disc ML-DmD16-c3  31 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11  (54) antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    19 ovary fGS/OSS  (34) ovary OSC  (33) ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    7 embryonic 1182-4H    24 embryonic GM2    37 embryonic Kc167    17 embryonic S1    26 embryonic S3    22 leg disc CME L1    10 wing disc CME-W2    17 wing disc ML-DmD8    15 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    19 ovary fGS/OSS    34 ovary OSC    33 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    7 embryonic 1182-4H    24 embryonic GM2    37 embryonic Kc167    17 embryonic S1    26 embryonic S3    22 leg disc CME L1    10 wing disc CME-W2    17 wing disc ML-DmD8    15 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    2 wing disc ML-DmD32    11 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    19 ovary fGS/OSS    34 ovary OSC    33 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    16 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_21 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • RE07981 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0037808 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6303-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Other Phenotypes Allele increased cell death BruceE16 BruceE81 Brucee00984 wild-type BrucedsRNA.GMR Phenotype manifest in Allele egg chamber BruceE16 BruceE81 Brucee00984
Classical Alleles ( 15 )
For All Classical Alleles Show
Allele of Bruce Class Mutagen Stocks Known lesion Brucee00984 piggyBac transposase 2 Yes Bruce10-1e 0 Yes Bruce8-1e 0 Yes Brucee00983 piggyBac transposase 0 -- BruceE12 P-element activity 0 Yes BruceE16 P-element activity 0 Yes BruceE23 0 Yes BruceE81 0 Yes BruceGMREP-86a-1 P-element activity 0 Yes BruceGMREP-86a-2 P-element activity 0 Yes BruceGMREP-86a-3 P-element activity 0 Yes BruceGMREP-86a-4 P-element activity 0 Yes BruceGMREP-86a-5 P-element activity 0 Yes BruceGS3144 0 -- Bruceunspecified 0 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Bruce Class Mutagen Stocks Known lesion BruceGD16949 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes BruceKK100132 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes BruceBIR.GMR in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes BruceBIR.Scer\UAS in vitro construct - regulatory fusion 0 Yes BrucedsRNA.GMR in vitro construct - RNAi 0 Yes BruceNIG.6303R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes BruceScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6156 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD16949} NA P{KK100132} NA P{NIG.6303R} NA P{UAS-Bruce.BIR} NA PBac{UAS-myc-Bruce} NA characterization construct P{GMR-Bruce.BIR} NA P{GMR-dBruce-RNAi} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}Brucee00983 NA PBac{RB}Brucee00984 NA insertion of mobile activating element P{GSV1}sleGS3144 NA P{Mae-GMR.E}BruceGMREP-86a-1 NA P{Mae-GMR.E}BruceGMREP-86a-2 NA P{Mae-GMR.E}BruceGMREP-86a-3 NA P{Mae-GMR.E}BruceGMREP-86a-4 NA P{Mae-GMR.E}BruceGMREP-86a-5 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
negative regulation of apoptotic process inferred from direct assay (Domingues and Ryoo, 2012) programmed cell death inferred from genetic interaction with grim AND inferred from genetic interaction with rpr (Vernooy et al., 2002) spermatid development inferred from mutant phenotype (Arama et al., 2003)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
small conjugating protein ligase activity traceable author statement (Bergmann et al., 2003) ubiquitin-protein ligase activity inferred from sequence or structural similarity (Vernooy et al., 2000) inferred from sequence or structural similarity with EMBL:Y17267 (FlyBase, 1992-)
Biological Process
CV term Evidence References
negative regulation of apoptotic process inferred from sequence or structural similarity with mouse Birc6 (Vernooy et al., 2000) sperm individualization non-traceable author statement (Arama et al., 2002)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Bruce - CG31199 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bruce - Cdc23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bruce - Jafrac2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bruce - gus anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bruce - klhl10 anti tag coimmunoprecipitation, western blot (Arama et al., 2007) Bruce - lok anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bruce - rpr pull down, anti tag western blot (Domingues and Ryoo, 2012)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Bruce allele Gene References BruceE12 rpr (Vernooy et al., 2002) BruceE16 rpr (Vernooy et al., 2002) BruceGMREP-86a-1 rpr (Vernooy et al., 2002) BruceGMREP-86a-2 rpr (Vernooy et al., 2002) Bruceunspecified rpr (Ryoo et al., 2002) unspecified rpr (Vernooy et al., 2002)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66407 DroID - A comprehensive database of gene and protein interactions. • FBgn0037808 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41260 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8MS47
Orthologs
OrthoDB Orthologs (65) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6DJKFQ Dipteran inclusive ortholog search EOG6JT0DB Insect inclusive ortholog search EOG6XGXTV Arthropod inclusive ortholog search EOG61ZCRN Metazoa inclusive ortholog search EOG6M3P24, EOG6QCD1N
Orthologs in Drosophila Species (EOG6DJKFQ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Bruce     Drosophila simulans   Dsim\GD20723 Y   Drosophila sechellia   Dsec\GM26173 Y   Drosophila sechellia   Dsec\GM16262     Drosophila erecta   Dere\GG17289 Y   Drosophila yakuba   Dyak\GE24690 Y   Drosophila ananassae   Dana\GF17464 Y   Drosophila pseudoobscura pseudoobscura   Dpse\Bruce Y   Drosophila persimilis   Dper\GL23835 Y   Drosophila willistoni   Dwil\GK14416 Y   Drosophila virilis   Dvir\GJ23661     Drosophila virilis   Dvir\GJ23660     Drosophila mojavensis   Dmoj\GI24031 Y   Drosophila grimshawi   Dgri\GH18453 Y
Orthologs in non-Drosophila Dipterans (EOG6JT0DB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012446   Anopheles gambiae Malaria mosquito  Agam\AGAP002651   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002689
Orthologs in non-Dipteran Insects (EOG6XGXTV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB44587   Apis mellifera Western honey bee  Amel\GB44588   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG023076   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH18678   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16021   Atta cephalotes Leafcutter ant  Acep\ACEP14733   Atta cephalotes Leafcutter ant  Acep\ACEP14732   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15321   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14166   Linepithema humile Argentine ant  Lhum\LH11323   Pogonomyrmex barbatus Red harvester ant  Pbar\PB20479   Solenopsis invicta Red fire ant  Sinv\SINV24437   Solenopsis invicta Red fire ant  Sinv\SINV18661   Bombyx mori Silkmoth  Bmor\BGIBMGA005071   Tribolium castaneum Red flour beetle  Tcas\TC009848
Orthologs in non-Insect Arthropods (EOG61ZCRN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_317519   Daphnia pulex Water flea  Dpux\JGI_V11_253206   Daphnia pulex Water flea  Dpux\JGI_V11_303455
Orthologs in non-Arthropod Metazoa (EOG6M3P24, EOG6QCD1N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ubc-17   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005908gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001262gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_013632gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005425gn   Danio rerio Zebrafish  Drer\birc6   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000013595   Xenopus tropicalis Western clawed frog  Xtro\birc6   Xenopus tropicalis Western clawed frog  Xtro\marcks   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000019170   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000020805   Gallus gallus Domestic chicken  Ggal\ENSGALG00000001071   Gallus gallus Domestic chicken  Ggal\ENSGALG00000019440   Gallus gallus Domestic chicken  Ggal\ENSGALG00000018512   Gallus gallus Domestic chicken  Ggal\BIRC6   Gallus gallus Domestic chicken  Ggal\LOC427882   Mus musculus House mouse  Mmus\Gm14188   Mus musculus House mouse  Mmus\Birc6   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000034110   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042970   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032468   Rattus norvegicus Norway rat  Rnor\Basp1   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000010965   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000036712   Rattus norvegicus Norway rat  Rnor\LOC688574   Rattus norvegicus Norway rat  Rnor\Tmem158   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042092   Rattus norvegicus Norway rat  Rnor\Lce1c   Rattus norvegicus Norway rat  Rnor\Birc6   Homo sapiens Human  Hsap\BIRC6
Human Orthologs (1)
Gene OMIM HGNC Hsap\BIRC6
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20723 Drosophila sechellia Dsec\GM26173 Drosophila erecta Dere\GG17289 Drosophila yakuba Dyak\GE24690 Drosophila ananassae Dana\GF17464 Drosophila pseudoobscura pseudoobscura Dpse\Bruce Drosophila persimilis Dper\GL23835 Drosophila willistoni Dwil\GK14416 Drosophila mojavensis Dmoj\GI24031 Drosophila grimshawi Dgri\GH18453
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	17911 w1118; PBac{RB}Brucee00984/TM6B, Tb1
Harvard	- PBac{RB}Brucee00984
VDRC	v107620 P{KK100132}VIE-260B v48309 w1118; P{GD16949}v48309/CyO
Genomic Clones ( 1 )
	BACR03L12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 195 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP05246 RE07981 SD20380
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT14384 GH10317 GM20514 GM22016 LD31268 LP15283 RH27177 RH51420 SD23747 UT01320
Other clones	28649 66118 72193 73118 121723 162998 214319.39 524718 CK01.64A.C10 CK01.100B.G1 CK01.106D.C2 CK01.128A.G1 Dmel_dig_004185_0611_1533 EC054854 EK024505 EK052043 EK077225 EK081241 EK098225 EK136733 EK166621 EK201135 EK202044 EK241853 EK267322 EN05810 EP07720 EP09945 EP10107 FGH120G12 RH36151 RP003002064 RP003009422 RP003012985 RP003022463 RP003022698 RP003026867 RP003029596 RP003036312 RP003036993 RP003042860 RP003058201 RP003060161 RP003069294 RP003079575 RP003083641 RP003085449 RP003104354 RP003110087 RP003113213 RP003113738 RP003115449 RP003116144 RP003123004 RP003124888 RP003129729 RP003132134 RP003138028 RP003139268 RP003144281 RP003148255 RP003150606 RP003154196 RP003162042 RP003162772 RP003162932 RP003172755 RP003177998 RP003178931 RP003189627 RP003195952 RP003198995 RP003201289 RP003202113 RP003206781 RP003214332 RP003216176 RP003218863 RP003219358 RP003232735 RP003241158 RP003245737 RP003250229 RP003257866 RP003267166 RP003268636 RP003271257 RP003275976 RP003281954 RP003283649 RP003284057 RP003286411 RP003287670 RP003287983 RP003291853 RP003293406 RP003294276 RP003299917 RP003300547 RP003302368 RP003303510 RP003304381 RP003305440 RP003306648 RP003307303 RP003309637 RP003314849 RP003321775 RP003321891 RP003324057 RP003324344 RP003324731 RP003331031 RP003335281 RP003343948 RP003349548 RP003350467 RP003350517 RP003357343 RP003357717 RP003358459 RP003375759 RP003376244 RP003376278 RP003388799 RP003396652 RP003413377 RP003418180 RP003428037 RP003429992 RP003432967 RP003440021 RP003445108 RP003464294 RP003469634 RP003478094 RP003480020 RP003480375 RP003485351 RP003499687 RP003500368 RP003501133 RP003503418 RP003506099 RP003509365 RP003509639 RP003516221 RP003516677 RP003520475 RP003524416 RP003524915 RP003525723 RP003526657 RP003536140 RP003536453 RP003539326 RP003542461 RP003542701 RP003543871 RP003546803 RP003550351 RP003551699 RP003555138 RP003556438 RP003556445 RP003556602 RP003559569 RP003562132 RP003564147 RP003564248 RP003572484 RP003589121 RP003625193 RP003629178 RP003652755 RP003653912 RP003667086 RP003669395 RP003674114 RP003677355 RP003678506 RP003682136
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0037808 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41260
Antibody Information
	polyclonal (Kaplan et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Bruce CG6303 (Vernooy et al., 2000) Source for identity of: Bruce CG6303 (Vernooy et al., 2000)
Source for database merge of
Additional comments
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Bruce can inhibit cell death induced by grim and rpr but not W. (Vernooy et al., 2002)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_141738 NM_001275531 NM_001275532 NM_001275533 NM_001275534 RefSeq (Proteins) • NP_649995 NP_001262460 NP_001262461 NP_001262462 NP_001262463 DDBJ / EMBL / GenBank • AAF54520 AAM50964 AAM77747 AF517634 AGB95842 AGB95843 AGB95844 AGB95845 AY119104 BI167572 CZ478812 CZ478813 Entrez Gene - A searchable database of RefSeq genes. • 41260 UniProtKB/TrEMBL • Q8MS47 Q9VH01
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • WD40-repeat-containing domain (IPR017986) Ubiquitin-conjugating enzyme, E2 (IPR000608) Baculoviral inhibition of apoptosis protein repeat (IPR001370) Ubiquitin-conjugating enzyme/RWD-like (IPR016135) Protein of unknown function DUF3643 (IPR022103) WD40/YVTN repeat-like-containing domain (IPR015943) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE07981
Linkouts
	BioGRID - A database of protein and genetic interactions • 66407 DroID - A comprehensive database of gene and protein interactions. • FBgn0037808 DRSC - Results from RNAi screens. • FBgn0037808 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0037808 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6303-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0037808 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41260 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/bruce.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41260 modMine - Data generated by the modENCODE project. • FBgn0037808 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8MS47
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	bruce (Ryoo et al., 2002, Ryoo et al., 2002) Bruce (Laviolette et al., 2005, Bartolome and Charlesworth, 2006, Jungreis, 2011.11.18, Hou et al., 2008, Carbone et al., 2009, Sathyanarayanan et al., 2008, Martin et al., 2007, Bader et al., 2010, Kaplan et al., 2010, Wasbrough et al., 2010, Wasbrough et al., 2010, Japanese National Institute of Genetics, 2012.5.21) BRUCE (Domingues and Ryoo, 2012) CG6303 (Vernooy et al., 2000, Vernooy et al., 2000, Laviolette et al., 2005, Keleman et al., 2009.8.5, Ying et al., 2011) dBruce (Orihara-Ono et al., 2005, Arama et al., 2003, Bergmann et al., 2003, Arama et al., 2001, Baehrecke, 2003, Gafuik and Steller, 2011, Kaplan et al., 2010, Nezis et al., 2010, Yoo, 2005) dbruce (Arama et al., 2002, Arama et al., 2007) dBRUCE (Vernooy et al., 2000, Vernooy et al., 2000)
Name Synonym	Bruce (Carbone et al., 2009, Peschel et al., 2009, Wasbrough et al., 2010) dbruce (Rathke et al., 2007)
Secondary FlyBase IDs
References ( 50 )
Generate a list of	All references relating to this gene ( 50 )
List References by type	Research paper  ( 23 ) Supplementary material  ( 5 ) Review  ( 3 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 2 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Domingues and Ryoo, 2012, Cell Death Differ. 19(3): 470--477 Drosophila BRUCE inhibits apoptosis through non-lysine ubiquitination of the IAP-antagonist REAPER. [FBrf0217448] Gafuik and Steller, 2011, PLoS ONE 6(8): e23535 A Gain-of-Function Germline Mutation in Drosophila ras1 Affects Apoptosis and Cell Fate during Development. [FBrf0214698] Ying et al., 2011, PLoS ONE 6(9): e23863 Comprehensively Surveying Structure and Function of RING Domains from Drosophila melanogaster. [FBrf0215242] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...