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Gene Dmel\Tsp86D

General Information
Symbol	Dmel\Tsp86D	Species	D. melanogaster
Name	Tetraspanin 86D	Annotation symbol	CG4591
Feature type	protein_coding_gene	FlyBase ID	FBgn0037848
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	86C7-86C7	Sequence location	3R:6,694,442..6,696,277 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Tetraspanin 86D is referred to in FlyBase by the symbol Dmel\Tsp86D (CG4591, FBgn0037848). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: nervous system development. 4 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Tetraspanin; Tetraspanin, EC2 domain; Tetraspanin, conserved site; Tetraspanin, subgroup. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1633758_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Tsp86D. Gene sequence location is 3R:6694442..6696277.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	References FlyBase Curators, 2010-, Gene Ontology (GO) annotation review.
FB2012_01	Sequence features HFA15607 BKN30509
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 86C7-86C7   Limits computationally determined from genome sequence between P{EP}EP3340EP3340 and P{PZ}tho1&P{PZ}l(3)0462905275
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Tsp86D for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Tsp86D-RA FBtr0082288  NM_079585   1779   291 Tsp86D-RB FBtr0306640     1389   291
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Tsp86D-PA   FBpp0081765   33.2   291   6.74     NP_524309   AAF54570 Tsp86D-PB   FBpp0297595   33.2   291   6.74
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Tetraspanin (IPR018499) Tetraspanin, conserved site (IPR018503) Tetraspanin, subgroup (IPR000301) Tetraspanin, EC2 domain (IPR008952)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567201   AE014297 AAF54570   AI531499   AI542745   AW941129   BI164357   BI167592   BI169944   BI171312   BI215240   BI216202   BI216897   BI240028   BI242211   BI483996   BI569843   BI569923   BI580195   BI589945   BI619640   BP553187   BP553188   BT004914 AAO49167   CO155178   CO155719   CO265668   CO271934   CO282723   CO282791   CO297645   CO301071   CO301097   CO304109   CO305669   CO333525   EC060434   EC062506   EC073578   EC077407   EC078235   EC197665   EC199620   EC202267   EC222915   EC223078   EC233390   EC236978   EC238246   EC261129   EC266547   GH755308   GH759773   GH759814   GH760909   GH775746   GH775802   GH780180   GH782715   GH784677   GH806817   GH878565   GH908135   GH920537   GH920885   GH927710   GH929273   GH933883
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VGV3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0037848    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0037848 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0037848 expression level Developmental Stage   Expression Level embryo 00-02hr    3219 embryo 02-04hr    3113 embryo 04-06hr    2195 embryo 06-08hr    3101 embryo 08-10hr    4226 embryo 10-12hr    5804 embryo 12-14hr    2864 embryo 14-16hr    2134 embryo 16-18hr    1597 embryo 18-20hr    1369 embryo 20-22hr    886 embryo 22-24hr    972 larva L1    907 larva L2    596 larva L3 12hr old    505 larva L3 puffstage 1-2    627 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1643 white prepupae new    2164 white prepupae 12hr    1993 white prepupae 24hr    2201 pupae 2d postWPP    1886 pupae 3d postWPP    1190 pupae 4d postWPP    754 adult male 01day    561 adult male 05day    624 adult male 30day    631 adult female 01day    1247 adult female 05day    1942 adult female 30day    2060 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3219) embryo 02-04hr  (3113) embryo 04-06hr  (2195) embryo 06-08hr  (3101) embryo 08-10hr  (4226) embryo 10-12hr  (5804) embryo 12-14hr  (2864) embryo 14-16hr  (2134) embryo 16-18hr  (1597) embryo 18-20hr  (1369) embryo 20-22hr  (886) embryo 22-24hr  (972) larva L1  (907) larva L2  (596) larva L3 12hr old  (505) larva L3 puffstage 1-2  (627) larva L3 puffstage 3-6  (1065) larva L3 puffstage 7-9  (1643) white prepupae new  (2164) white prepupae 12hr  (1993) white prepupae 24hr  (2201) pupae 2d postWPP  (1886) pupae 3d postWPP  (1190) pupae 4d postWPP  (754) adult male 01day  (561) adult male 05day  (624) adult male 30day  (631) adult female 01day  (1247) adult female 05day  (1942) adult female 30day  (2060) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3219) embryo 02-04hr  (3113) embryo 04-06hr  (2195) embryo 06-08hr  (3101) embryo 08-10hr  (4226) embryo 10-12hr  (5804) embryo 12-14hr  (2864) embryo 14-16hr  (2134) embryo 16-18hr  (1597) embryo 18-20hr    1369 embryo 20-22hr    886 embryo 22-24hr    972 larva L1    907 larva L2    596 larva L3 12hr old    505 larva L3 puffstage 1-2    627 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9  (1643) white prepupae new  (2164) white prepupae 12hr  (1993) white prepupae 24hr  (2201) pupae 2d postWPP  (1886) pupae 3d postWPP    1190 pupae 4d postWPP    754 adult male 01day    561 adult male 05day    624 adult male 30day    631 adult female 01day    1247 adult female 05day  (1942) adult female 30day  (2060) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3219 embryo 02-04hr    3113 embryo 04-06hr    2195 embryo 06-08hr    3101 embryo 08-10hr    4226 embryo 10-12hr    5804 embryo 12-14hr    2864 embryo 14-16hr    2134 embryo 16-18hr    1597 embryo 18-20hr    1369 embryo 20-22hr    886 embryo 22-24hr    972 larva L1    907 larva L2    596 larva L3 12hr old    505 larva L3 puffstage 1-2    627 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1643 white prepupae new    2164 white prepupae 12hr    1993 white prepupae 24hr    2201 pupae 2d postWPP    1886 pupae 3d postWPP    1190 pupae 4d postWPP    754 adult male 01day    561 adult male 05day    624 adult male 30day    631 adult female 01day    1247 adult female 05day    1942 adult female 30day    2060 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3219 embryo 02-04hr    3113 embryo 04-06hr    2195 embryo 06-08hr    3101 embryo 08-10hr    4226 embryo 10-12hr    5804 embryo 12-14hr    2864 embryo 14-16hr    2134 embryo 16-18hr    1597 embryo 18-20hr    1369 embryo 20-22hr    886 embryo 22-24hr    972 larva L1    907 larva L2    596 larva L3 12hr old    505 larva L3 puffstage 1-2    627 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1643 white prepupae new    2164 white prepupae 12hr    1993 white prepupae 24hr    2201 pupae 2d postWPP    1886 pupae 3d postWPP    1190 pupae 4d postWPP    754 adult male 01day    561 adult male 05day    624 adult male 30day    631 adult female 01day    1247 adult female 05day    1942 adult female 30day    2060 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0037848 expression level Developmental Stage   Expression Level embryo 00-02hr    3219 embryo 02-04hr    3113 embryo 04-06hr    2195 embryo 06-08hr    3101 embryo 08-10hr    4226 embryo 10-12hr    5804 embryo 12-14hr    2864 embryo 14-16hr    2134 embryo 16-18hr    1597 embryo 18-20hr    1369 embryo 20-22hr    886 embryo 22-24hr    972 larva L1    907 larva L2    596 larva L3 12hr old    505 larva L3 puffstage 1-2    627 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1643 white prepupae new    2164 white prepupae 12hr    1993 white prepupae 24hr    2201 pupae 2d postWPP    1886 pupae 3d postWPP    1190 pupae 4d postWPP    754 adult male 01day    561 adult male 05day    624 adult male 30day    631 adult female 01day    1247 adult female 05day    1942 adult female 30day    2060 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3219) embryo 02-04hr  (3113) embryo 04-06hr  (2195) embryo 06-08hr  (3101) embryo 08-10hr  (4226) embryo 10-12hr  (5804) embryo 12-14hr  (2864) embryo 14-16hr  (2134) embryo 16-18hr  (1597) embryo 18-20hr  (1369) embryo 20-22hr  (886) embryo 22-24hr  (972) larva L1  (907) larva L2  (596) larva L3 12hr old  (505) larva L3 puffstage 1-2  (627) larva L3 puffstage 3-6  (1065) larva L3 puffstage 7-9  (1643) white prepupae new  (2164) white prepupae 12hr  (1993) white prepupae 24hr  (2201) pupae 2d postWPP  (1886) pupae 3d postWPP  (1190) pupae 4d postWPP  (754) adult male 01day  (561) adult male 05day  (624) adult male 30day  (631) adult female 01day  (1247) adult female 05day  (1942) adult female 30day  (2060) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3219) embryo 02-04hr  (3113) embryo 04-06hr  2195 embryo 06-08hr  (3101) embryo 08-10hr  (4226) embryo 10-12hr  (5804) embryo 12-14hr  (2864) embryo 14-16hr  2134 embryo 16-18hr  1597 embryo 18-20hr    1369 embryo 20-22hr    886 embryo 22-24hr    972 larva L1    907 larva L2    596 larva L3 12hr old    505 larva L3 puffstage 1-2    627 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9  1643 white prepupae new  2164 white prepupae 12hr  1993 white prepupae 24hr  2201 pupae 2d postWPP  1886 pupae 3d postWPP    1190 pupae 4d postWPP    754 adult male 01day    561 adult male 05day    624 adult male 30day    631 adult female 01day    1247 adult female 05day  1942 adult female 30day  2060 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3219 embryo 02-04hr    3113 embryo 04-06hr    2195 embryo 06-08hr    3101 embryo 08-10hr    4226 embryo 10-12hr    5804 embryo 12-14hr    2864 embryo 14-16hr    2134 embryo 16-18hr    1597 embryo 18-20hr    1369 embryo 20-22hr    886 embryo 22-24hr    972 larva L1    907 larva L2    596 larva L3 12hr old    505 larva L3 puffstage 1-2    627 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1643 white prepupae new    2164 white prepupae 12hr    1993 white prepupae 24hr    2201 pupae 2d postWPP    1886 pupae 3d postWPP    1190 pupae 4d postWPP    754 adult male 01day    561 adult male 05day    624 adult male 30day    631 adult female 01day    1247 adult female 05day    1942 adult female 30day    2060 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3219 embryo 02-04hr    3113 embryo 04-06hr    2195 embryo 06-08hr    3101 embryo 08-10hr    4226 embryo 10-12hr    5804 embryo 12-14hr    2864 embryo 14-16hr    2134 embryo 16-18hr    1597 embryo 18-20hr    1369 embryo 20-22hr    886 embryo 22-24hr    972 larva L1    907 larva L2    596 larva L3 12hr old    505 larva L3 puffstage 1-2    627 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1643 white prepupae new    2164 white prepupae 12hr    1993 white prepupae 24hr    2201 pupae 2d postWPP    1886 pupae 3d postWPP    1190 pupae 4d postWPP    754 adult male 01day    561 adult male 05day    624 adult male 30day    631 adult female 01day    1247 adult female 05day    1942 adult female 30day    2060 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0037848    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0037848 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0037848 expression level Tissue   Expression Level Larval Central Nervous System    66.425 Larval Midgut    44.8 Larval Hindgut    38.6 Larval Malpighian Tubules    46.4 Larval Fat Body    34.1 Larval Salivary Gland    78.4 Larval Trachea    54 Larval Carcass    40.7 Adult Head    28.5 Adult Eye    26.075 Adult Brain    79.5 Adult Thoracic-Abdominal Ganglion    51.7 Adult Crop    22.3 Adult Midgut    53.8 Adult Hindgut    28.5 Adult Malpighian Tubules no informative data Adult Fat Body    24.7 Adult Salivary Gland no informative data Adult Heart    65.35 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    14.7 Adult Ovary    73 Adult Testis    27.1 Adult Male Accessory Gland no informative data Adult Carcass    30.6 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    66.425 Larval Midgut    44.8 Larval Hindgut    38.6 Larval Malpighian Tubules    46.4 Larval Fat Body    34.1 Larval Salivary Gland    78.4 Larval Trachea    54 Larval Carcass    40.7 Adult Head    28.5 Adult Eye    26.075 Adult Brain    79.5 Adult Thoracic-Abdominal Ganglion    51.7 Adult Crop    22.3 Adult Midgut    53.8 Adult Hindgut    28.5 Adult Malpighian Tubules no informative data Adult Fat Body    24.7 Adult Salivary Gland no informative data Adult Heart    65.35 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    14.7 Adult Ovary    73 Adult Testis    27.1 Adult Male Accessory Gland no informative data Adult Carcass    30.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    66.425 Larval Midgut    44.8 Larval Hindgut    38.6 Larval Malpighian Tubules    46.4 Larval Fat Body    34.1 Larval Salivary Gland    78.4 Larval Trachea    54 Larval Carcass    40.7 Adult Head    28.5 Adult Eye    26.075 Adult Brain    79.5 Adult Thoracic-Abdominal Ganglion    51.7 Adult Crop    22.3 Adult Midgut    53.8 Adult Hindgut    28.5 Adult Malpighian Tubules no informative data Adult Fat Body    24.7 Adult Salivary Gland no informative data Adult Heart    65.35 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    14.7 Adult Ovary    73 Adult Testis    27.1 Adult Male Accessory Gland no informative data Adult Carcass    30.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    66.425 Larval Midgut    44.8 Larval Hindgut    38.6 Larval Malpighian Tubules    46.4 Larval Fat Body    34.1 Larval Salivary Gland    78.4 Larval Trachea    54 Larval Carcass    40.7 Adult Head    28.5 Adult Eye    26.075 Adult Brain    79.5 Adult Thoracic-Abdominal Ganglion    51.7 Adult Crop    22.3 Adult Midgut    53.8 Adult Hindgut    28.5 Adult Malpighian Tubules no informative data Adult Fat Body    24.7 Adult Salivary Gland no informative data Adult Heart    65.35 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    14.7 Adult Ovary    73 Adult Testis    27.1 Adult Male Accessory Gland no informative data Adult Carcass    30.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0037848 expression level Tissue   Expression Level Larval Central Nervous System    66.425 Larval Midgut    44.8 Larval Hindgut    38.6 Larval Malpighian Tubules    46.4 Larval Fat Body    34.1 Larval Salivary Gland    78.4 Larval Trachea    54 Larval Carcass    40.7 Adult Head    28.5 Adult Eye    26.075 Adult Brain    79.5 Adult Thoracic-Abdominal Ganglion    51.7 Adult Crop    22.3 Adult Midgut    53.8 Adult Hindgut    28.5 Adult Malpighian Tubules no informative data Adult Fat Body    24.7 Adult Salivary Gland no informative data Adult Heart    65.35 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    14.7 Adult Ovary    73 Adult Testis    27.1 Adult Male Accessory Gland no informative data Adult Carcass    30.6 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    66.425 Larval Midgut    44.8 Larval Hindgut    38.6 Larval Malpighian Tubules    46.4 Larval Fat Body    34.1 Larval Salivary Gland    78.4 Larval Trachea    54 Larval Carcass    40.7 Adult Head    28.5 Adult Eye    26.075 Adult Brain    79.5 Adult Thoracic-Abdominal Ganglion    51.7 Adult Crop    22.3 Adult Midgut    53.8 Adult Hindgut    28.5 Adult Malpighian Tubules no informative data Adult Fat Body    24.7 Adult Salivary Gland no informative data Adult Heart    65.35 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    14.7 Adult Ovary    73 Adult Testis    27.1 Adult Male Accessory Gland no informative data Adult Carcass    30.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    66.425 Larval Midgut    44.8 Larval Hindgut    38.6 Larval Malpighian Tubules    46.4 Larval Fat Body    34.1 Larval Salivary Gland    78.4 Larval Trachea    54 Larval Carcass    40.7 Adult Head    28.5 Adult Eye    26.075 Adult Brain    79.5 Adult Thoracic-Abdominal Ganglion    51.7 Adult Crop    22.3 Adult Midgut    53.8 Adult Hindgut    28.5 Adult Malpighian Tubules no informative data Adult Fat Body    24.7 Adult Salivary Gland no informative data Adult Heart    65.35 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    14.7 Adult Ovary    73 Adult Testis    27.1 Adult Male Accessory Gland no informative data Adult Carcass    30.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    66.425 Larval Midgut    44.8 Larval Hindgut    38.6 Larval Malpighian Tubules    46.4 Larval Fat Body    34.1 Larval Salivary Gland    78.4 Larval Trachea    54 Larval Carcass    40.7 Adult Head    28.5 Adult Eye    26.075 Adult Brain    79.5 Adult Thoracic-Abdominal Ganglion    51.7 Adult Crop    22.3 Adult Midgut    53.8 Adult Hindgut    28.5 Adult Malpighian Tubules no informative data Adult Fat Body    24.7 Adult Salivary Gland no informative data Adult Heart    65.35 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    14.7 Adult Ovary    73 Adult Testis    27.1 Adult Male Accessory Gland no informative data Adult Carcass    30.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    28.5   NA  Eye    26.075   NA  Brain    79.5   66.425  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    51.7   NA  Crop    22.3   44.8  Midgut    53.8   38.6  Hindgut    28.5   46.4  Malpighian Tubules    no informative data   34.1  Fat Body    24.7   78.4  Salivary Gland    no informative data   NA  Heart    65.35   54  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    14.7   NA  Ovary    73   NA  Testis    27.1   NA  Male Accessory Gland    no informative data   40.7  Carcass    30.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_30
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0037848 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4591-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Tsp86DGD2904 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Tsp86D Class Mutagen Stocks Known lesion Tsp86Df00444 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Tsp86D Class Mutagen Stocks Known lesion Tsp86DGD2904 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Tsp86DHM05001 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Tsp86DKK110509 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6159 (Ryder, 2004.4.26) Df(3R)Exel7305 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD2904} NA P{KK110509} NA P{TRiP.HM05001} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}Tsp86Df00444 NA
Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )
	There is no information supporting a molecular function term for this gene. (Based on a review of available data on: 2011-06-28)
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
nervous system development inferred from mutant phenotype (Koizumi et al., 2007)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR000301, InterPro:IPR008952, InterPro:IPR018499, InterPro:IPR018503 (FlyBase Curators et al., 2004-) inferred from sequence model (Fradkin et al., 2002) inferred from sequence or structural similarity (Todres et al., 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Tsp86D allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66454 DroID - A comprehensive database of gene and protein interactions. • FBgn0037848 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41310
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18839 Dere\GG17792 Dgri\GH18862 Dmoj\GI22932 Dper\GL12547 Dpse\GA18282 Dsec\GM23909 Dsim\GD18721 Dvir\GJ23216 Dwil\GK13989 Dyak\GE26059
Curated drosophilid orthologs	Dpse\GA18282
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL004147-PA Aedes aegypti (mosquito) AGAP002257-PA Anopheles gambiae (mosquito) 58873 Branchiostoma floridae (lancelet) BGIBMGA010813-PA Bombyx mori (silk worm) ENSBTAP00000009542 Bos taurus (bovine) ENSCAFP00000002214 Canis familiaris (dog) JA04778 Caenorhabditis japonica (nematode) CPIJ013692 Culex pipens (mosquito) ENSCPOP00000015562 Cavia porcellus (guinea pig) 132011 Capitella spl (polychaete worm) ENSDARP00000007865 Danio rerio (zebra fish) ENSGACP00000017939 Gymnopilus aculeatus (mushroom) ENSP00000289407 Homo sapiens (human) 160862 Lottia gigantea (owl limpet) ENSMODP00000018355 Monodelphis domestica (gray opossum) ENSMMUP00000015877 Macaca mulatta (rhesus monkey) ENSMUSP00000110905 Mus musculus (house mouse) ENSOANP00000018140 Ornithorynchus anatinus (platypus) PHUM005446-PA Pediculus humanus (human body louse) PP06817 Pristionchus pacificus (nematode) ENSPPYP00000020185 Pongo pygmaeus (orangutan) ENSPTRP00000033704 Pan troglodytes (chimpanzee) ENSRNOP00000010989 Rattus norvegicus (norway rat) Smp_035020.2 Schistosoma mansoni (trematode parasite) 64199 Trichoplax adhaerens (metazoa) XP_966752.1 Tribolium castaneum (red flour beetle) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV19106 LH16672 ISCW024092 ISCW015408 ISCW013461 ISCW009389 ISCW008421 ISCW000533 FBgn0243101 FBgn0234422 FBgn0215995 FBgn0210318 FBgn0206348 FBgn0195936 FBgn0190242 FBgn0178774 FBgn0173772 JGI_V11_303081 FBgn0244053 FBgn0078287 FBgn0164463 FBgn0150153 FBgn0145659 FBgn0138417 FBgn0040334 FBgn0126329 FBgn0119359 FBgn0110014 FBgn0104891 FBgn0098930 FBgn0095856 CPIJ013692 BGIBMGA010813 ACYPI003535 GB12911 AGAP002257 AAEL004147 PB22526 PHUM433300 GLEAN_12071
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	28515 y1 v1; P{TRiP.HM05001}attP2
Harvard	- PBac{WH}Tsp86Df00444
VDRC	v102046 P{KK110509}VIE-260B v42642 w1118; P{GD2904}v42642 v42643 w1118; P{GD2904}v42643
Genomic Clones ( 2 )
	BACR15J23 BACR32N16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 63 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM01305
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE04020 RE08010 RE11264 RE13055 RE21583 RE23144 RE24180 RE36671 RE39487 RE66996 RE74343 RH02007 RH02125 RH03471 RH50715 SD02565 SD09195
Other clones	251 9950 33306 45394 45466 54514 54610 56816 102022 104481 146611 156233 166098 524506 525508 EK105228 EK143340 EK161040 EK161223 EK170251 EK180017 EK180050 EK243744 EK249221 EK307202 EN05523 EN06623 FGM223A08 RP003000505 RP003040023 RP003141147 RP003168729 RP003168960 RP003212742 RP003268754 RP003300589 RP003305896 RP003330455 RP003332321 RP003369081 RP003394401 RP003411591 RP003412227 RP003435453 RP003513142
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0037848 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41310
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Tsp86D CG4591 (Todres et al., 2000)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079585 RefSeq (Proteins) • NP_524309 DDBJ / EMBL / GenBank • AA567201 AAF54570 AAO49167 AW941129 BT004914 Entrez Gene - A searchable database of RefSeq genes. • 41310 UniProtKB/TrEMBL • Q9VGV3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Tetraspanin (IPR018499) Tetraspanin, conserved site (IPR018503) Tetraspanin, subgroup (IPR000301) Tetraspanin, EC2 domain (IPR008952)
Linkouts
	BioGRID - A database of protein and genetic interactions • 66454 DroID - A comprehensive database of gene and protein interactions. • FBgn0037848 DRSC - Results from RNAi screens. • FBgn0037848 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0037848 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4591-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0037848 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41310 InParanoid A subset of ortholog calls from InParanoid. • AAEL004147-PA Aedes aegypti (mosquito) AGAP002257-PA Anopheles gambiae (mosquito) 58873 Branchiostoma floridae (lancelet) BGIBMGA010813-PA Bombyx mori (silk worm) ENSBTAP00000009542 Bos taurus (bovine) ENSCAFP00000002214 Canis familiaris (dog) JA04778 Caenorhabditis japonica (nematode) CPIJ013692 Culex pipens (mosquito) ENSCPOP00000015562 Cavia porcellus (guinea pig) 132011 Capitella spl (polychaete worm) ENSDARP00000007865 Danio rerio (zebra fish) ENSGACP00000017939 Gymnopilus aculeatus (mushroom) ENSP00000289407 Homo sapiens (human) 160862 Lottia gigantea (owl limpet) ENSMODP00000018355 Monodelphis domestica (gray opossum) ENSMMUP00000015877 Macaca mulatta (rhesus monkey) ENSMUSP00000110905 Mus musculus (house mouse) ENSOANP00000018140 Ornithorynchus anatinus (platypus) PHUM005446-PA Pediculus humanus (human body louse) PP06817 Pristionchus pacificus (nematode) ENSPPYP00000020185 Pongo pygmaeus (orangutan) ENSPTRP00000033704 Pan troglodytes (chimpanzee) ENSRNOP00000010989 Rattus norvegicus (norway rat) Smp_035020.2 Schistosoma mansoni (trematode parasite) 64199 Trichoplax adhaerens (metazoa) XP_966752.1 Tribolium castaneum (red flour beetle) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41310 modMine - Data generated by the modENCODE project. • FBgn0037848 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV19106 LH16672 ISCW024092 ISCW015408 ISCW013461 ISCW009389 ISCW008421 ISCW000533 FBgn0243101 FBgn0234422 FBgn0215995 FBgn0210318 FBgn0206348 FBgn0195936 FBgn0190242 FBgn0178774 FBgn0173772 JGI_V11_303081 FBgn0244053 FBgn0078287 FBgn0164463 FBgn0150153 FBgn0145659 FBgn0138417 FBgn0040334 FBgn0126329 FBgn0119359 FBgn0110014 FBgn0104891 FBgn0098930 FBgn0095856 CPIJ013692 BGIBMGA010813 ACYPI003535 GB12911 AGAP002257 AAEL004147 PB22526 PHUM433300 GLEAN_12071
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	CG4591 (Fradkin et al., 2002, Seigneuret et al., 2001, Todres et al., 2000, Keleman et al., 2009.8.5, Perkins et al., 2009) Dm.Tsp86D (Todres et al., 2000) Tsp86D (Koizumi et al., 2007)
Name Synonym	Tetraspanin 86D (Todres et al., 2000)
Secondary FlyBase IDs
References ( 19 )
Research paper	Koizumi et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(13): 5626--5631 RNA interference screen to identify genes required for Drosophila embryonic nervous system development. [FBrf0200690] Fradkin et al., 2002, Proc. Natl. Acad. Sci. USA 99(21): 13663--13668 Genomewide analysis of the Drosophila tetraspanins reveals a subset with similar function in the formation of the embryonic synapse. [FBrf0152316] Seigneuret et al., 2001, J. Biol. Chem. 276(43): 40055--40064 Structure of the tetraspanin main extracellular domain. A partially conserved fold with a structurally variable domain insertion. [FBrf0145600] Todres et al., 2000, Insect Molec. Biol. 9(6): 581--590 The tetraspanin superfamily in insects. [FBrf0132356]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Perkins et al., 2009, Update to TRiP stock collection. Update to TRiP stock collection. [FBrf0208864] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335]
FlyBase analysis	FlyBase Curators, 2010-, Gene Ontology (GO) annotation review. Gene Ontology (GO) annotation review. [FBrf0216433] FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]
Automatic genome annotation	FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126705]
Curated genome annotation	Heiman, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126693]