Gene Dmel\CG5276

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General Information

Symbol

Dmel\CG5276

Species

D. melanogaster

Name

Annotation symbol

CG5276

Feature type

protein_coding_gene

FlyBase ID

FBgn0037900

Gene Model Status

Current

Stock availability

4 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

86E5-86E5

Sequence location

3R:7,257,460..7,259,265 [+]

Genomic Maps

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Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG5276 (FBgn0037900). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: nucleoside-triphosphatase activity; nucleoside-diphosphatase activity. The biological processes in which it is involved are not known. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Apyrase. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval/adult salivary gland, adult male reproductive system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval trachea, adult female reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1636403_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG5276. Gene sequence location is 3R:7257460..7259265.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

86E5-86E5

Limits computationally determined from genome sequence between P{EP}EP3340^EP3340 and P{PZ}tho¹&P{PZ}l(3)04629⁰⁵²⁷⁵

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG5276 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG5276-RA

FBtr0082390

NM_141817

1752

419

CG5276-RB

FBtr0339375

1608

419

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG5276-PA

FBpp0081866

47.7

419

6.73

NP_650074

AAF54638

CG5276-PB

FBpp0308466

47.7

419

6.73

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Apyrase (IPR009283)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VGN8

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval/adult salivary gland, adult male reproductive system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval trachea, adult female reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		106.25
Larval Midgut		117.1
Larval Hindgut		140.5
Larval Malpighian Tubules		179.5
Larval Fat Body		154
Larval Salivary Gland		1002.9
Larval Trachea		128.325
Larval Carcass		157.75
Adult Head		106.8
Adult Eye		79.45
Adult Brain		143.4
Adult Thoracic-Abdominal Ganglion		142.5
Adult Crop		181.3
Adult Midgut		137.4
Adult Hindgut		148.8
Adult Malpighian Tubules		172.6
Adult Fat Body		83.8
Adult Salivary Gland		1477.4
Adult Heart		103.175
Adult VirginFemale Spermatheca		95.2
Adult InseminatedFemale Spermatheca		104.7
Adult Ovary		181.9
Adult Testis		848.9
Adult Male Accessory Gland		631.2
Adult Carcass		131.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		106.25
Larval Midgut		117.1
Larval Hindgut		140.5
Larval Malpighian Tubules		179.5
Larval Fat Body		154
Larval Salivary Gland		(1002.9)
Larval Trachea		128.325
Larval Carcass		157.75
Adult Head		106.8
Adult Eye		79.45
Adult Brain		143.4
Adult Thoracic-Abdominal Ganglion		142.5
Adult Crop		181.3
Adult Midgut		137.4
Adult Hindgut		148.8
Adult Malpighian Tubules		172.6
Adult Fat Body		83.8
Adult Salivary Gland		(1477.4)
Adult Heart		103.175
Adult VirginFemale Spermatheca		95.2
Adult InseminatedFemale Spermatheca		104.7
Adult Ovary		181.9
Adult Testis		(848.9)
Adult Male Accessory Gland		(631.2)
Adult Carcass		131.8
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		106.25
Larval Midgut		117.1
Larval Hindgut		140.5
Larval Malpighian Tubules		179.5
Larval Fat Body		154
Larval Salivary Gland		1002.9
Larval Trachea		128.325
Larval Carcass		157.75
Adult Head		106.8
Adult Eye		79.45
Adult Brain		143.4
Adult Thoracic-Abdominal Ganglion		142.5
Adult Crop		181.3
Adult Midgut		137.4
Adult Hindgut		148.8
Adult Malpighian Tubules		172.6
Adult Fat Body		83.8
Adult Salivary Gland		(1477.4)
Adult Heart		103.175
Adult VirginFemale Spermatheca		95.2
Adult InseminatedFemale Spermatheca		104.7
Adult Ovary		181.9
Adult Testis		848.9
Adult Male Accessory Gland		631.2
Adult Carcass		131.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		106.25
Larval Midgut		117.1
Larval Hindgut		140.5
Larval Malpighian Tubules		179.5
Larval Fat Body		154
Larval Salivary Gland		1002.9
Larval Trachea		128.325
Larval Carcass		157.75
Adult Head		106.8
Adult Eye		79.45
Adult Brain		143.4
Adult Thoracic-Abdominal Ganglion		142.5
Adult Crop		181.3
Adult Midgut		137.4
Adult Hindgut		148.8
Adult Malpighian Tubules		172.6
Adult Fat Body		83.8
Adult Salivary Gland		1477.4
Adult Heart		103.175
Adult VirginFemale Spermatheca		95.2
Adult InseminatedFemale Spermatheca		104.7
Adult Ovary		181.9
Adult Testis		848.9
Adult Male Accessory Gland		631.2
Adult Carcass		131.8
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		106.25
Larval Midgut		117.1
Larval Hindgut		140.5
Larval Malpighian Tubules		179.5
Larval Fat Body		154
Larval Salivary Gland		1002.9
Larval Trachea		128.325
Larval Carcass		157.75
Adult Head		106.8
Adult Eye		79.45
Adult Brain		143.4
Adult Thoracic-Abdominal Ganglion		142.5
Adult Crop		181.3
Adult Midgut		137.4
Adult Hindgut		148.8
Adult Malpighian Tubules		172.6
Adult Fat Body		83.8
Adult Salivary Gland		1477.4
Adult Heart		103.175
Adult VirginFemale Spermatheca		95.2
Adult InseminatedFemale Spermatheca		104.7
Adult Ovary		181.9
Adult Testis		848.9
Adult Male Accessory Gland		631.2
Adult Carcass		131.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		106.25
Larval Midgut		117.1
Larval Hindgut		140.5
Larval Malpighian Tubules		179.5
Larval Fat Body		154
Larval Salivary Gland		(1002.9)
Larval Trachea		128.325
Larval Carcass		157.75
Adult Head		106.8
Adult Eye		79.45
Adult Brain		143.4
Adult Thoracic-Abdominal Ganglion		142.5
Adult Crop		181.3
Adult Midgut		137.4
Adult Hindgut		148.8
Adult Malpighian Tubules		172.6
Adult Fat Body		83.8
Adult Salivary Gland		(1477.4)
Adult Heart		103.175
Adult VirginFemale Spermatheca		95.2
Adult InseminatedFemale Spermatheca		104.7
Adult Ovary		181.9
Adult Testis		(848.9)
Adult Male Accessory Gland		631.2
Adult Carcass		131.8
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		106.25
Larval Midgut		117.1
Larval Hindgut		140.5
Larval Malpighian Tubules		179.5
Larval Fat Body		154
Larval Salivary Gland		1002.9
Larval Trachea		128.325
Larval Carcass		157.75
Adult Head		106.8
Adult Eye		79.45
Adult Brain		143.4
Adult Thoracic-Abdominal Ganglion		142.5
Adult Crop		181.3
Adult Midgut		137.4
Adult Hindgut		148.8
Adult Malpighian Tubules		172.6
Adult Fat Body		83.8
Adult Salivary Gland		1477.4
Adult Heart		103.175
Adult VirginFemale Spermatheca		95.2
Adult InseminatedFemale Spermatheca		104.7
Adult Ovary		181.9
Adult Testis		848.9
Adult Male Accessory Gland		631.2
Adult Carcass		131.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		106.25
Larval Midgut		117.1
Larval Hindgut		140.5
Larval Malpighian Tubules		179.5
Larval Fat Body		154
Larval Salivary Gland		1002.9
Larval Trachea		128.325
Larval Carcass		157.75
Adult Head		106.8
Adult Eye		79.45
Adult Brain		143.4
Adult Thoracic-Abdominal Ganglion		142.5
Adult Crop		181.3
Adult Midgut		137.4
Adult Hindgut		148.8
Adult Malpighian Tubules		172.6
Adult Fat Body		83.8
Adult Salivary Gland		1477.4
Adult Heart		103.175
Adult VirginFemale Spermatheca		95.2
Adult InseminatedFemale Spermatheca		104.7
Adult Ovary		181.9
Adult Testis		848.9
Adult Male Accessory Gland		631.2
Adult Carcass		131.8
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	106.8
NA	Eye	79.45
NA	Brain	143.4
106.25	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	142.5
NA	Crop	181.3
117.1	Midgut	137.4
140.5	Hindgut	148.8
179.5	Malpighian Tubules	172.6
154	Fat Body	83.8
1002.9	Salivary Gland	1477.4
NA	Heart	103.175
128.325	Trachea	NA
NA	VirginFemale Spermatheca	95.2
NA	InseminatedFemale Spermatheca	104.7
NA	Ovary	181.9
NA	Testis	848.9
NA	Male Accessory Gland	631.2
157.75	Carcass	131.8

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		6
head, mated 1-day female		2
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		5
head, mated 20-day male		7
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		6
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		7
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		5
ovary, virgin 4-day female		12
ovary, mated 4-day female		10
testis, mated 4-day male		115
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(34)
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		6
head, mated 1-day female		2
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		5
head, mated 20-day male		7
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		6
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		7
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		5
ovary, virgin 4-day female		12
ovary, mated 4-day female		10
testis, mated 4-day male		(115)
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		6
head, mated 1-day female		2
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		5
head, mated 20-day male		7
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		6
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		7
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		5
ovary, virgin 4-day female		12
ovary, mated 4-day female		10
testis, mated 4-day male		(115)
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		6
head, mated 1-day female		2
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		5
head, mated 20-day male		7
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		6
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		7
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		5
ovary, virgin 4-day female		12
ovary, mated 4-day female		10
testis, mated 4-day male		115
accessory gland, mated 4-day male		26
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		6
head, mated 1-day female		2
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		5
head, mated 20-day male		7
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		6
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		7
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		5
ovary, virgin 4-day female		12
ovary, mated 4-day female		10
testis, mated 4-day male		115
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(34)
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		6
head, mated 1-day female		2
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		5
head, mated 20-day male		7
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		6
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		7
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		5
ovary, virgin 4-day female		12
ovary, mated 4-day female		10
testis, mated 4-day male		(115)
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		6
head, mated 1-day female		2
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		5
head, mated 20-day male		7
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		6
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		7
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		5
ovary, virgin 4-day female		12
ovary, mated 4-day female		10
testis, mated 4-day male		115
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		3
central nervous system, pupae P8		3
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		6
head, mated 1-day female		2
head, mated 4-day female		4
head, mated 20-day female		4
head, mated 1-day male		6
head, mated 4-day male		5
head, mated 20-day male		7
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		6
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		7
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		5
ovary, virgin 4-day female		12
ovary, mated 4-day female		10
testis, mated 4-day male		115
accessory gland, mated 4-day male		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed in adult male stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		26
embryo 02-04hr		21
embryo 04-06hr		18
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		18
embryo 12-14hr		16
embryo 14-16hr		14
embryo 16-18hr		12
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		8
larva L1		10
larva L2		12
larva L3 12hr old		10
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		18
white prepupae new		19
white prepupae 12hr		19
white prepupae 24hr		23
pupae 2d postWPP		27
pupae 3d postWPP		29
pupae 4d postWPP		34
adult male 01day		56
adult male 05day		67
adult male 30day		73
adult female 01day		14
adult female 05day		17
adult female 30day		19
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		26
embryo 02-04hr		21
embryo 04-06hr		18
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		18
embryo 12-14hr		16
embryo 14-16hr		14
embryo 16-18hr		12
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		8
larva L1		10
larva L2		12
larva L3 12hr old		10
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		18
white prepupae new		19
white prepupae 12hr		19
white prepupae 24hr		23
pupae 2d postWPP		(27)
pupae 3d postWPP		(29)
pupae 4d postWPP		(34)
adult male 01day		(56)
adult male 05day		(67)
adult male 30day		(73)
adult female 01day		14
adult female 05day		17
adult female 30day		19
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		26
embryo 02-04hr		21
embryo 04-06hr		18
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		18
embryo 12-14hr		16
embryo 14-16hr		14
embryo 16-18hr		12
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		8
larva L1		10
larva L2		12
larva L3 12hr old		10
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		18
white prepupae new		19
white prepupae 12hr		19
white prepupae 24hr		23
pupae 2d postWPP		27
pupae 3d postWPP		29
pupae 4d postWPP		34
adult male 01day		56
adult male 05day		67
adult male 30day		73
adult female 01day		14
adult female 05day		17
adult female 30day		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		26
embryo 02-04hr		21
embryo 04-06hr		18
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		18
embryo 12-14hr		16
embryo 14-16hr		14
embryo 16-18hr		12
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		8
larva L1		10
larva L2		12
larva L3 12hr old		10
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		18
white prepupae new		19
white prepupae 12hr		19
white prepupae 24hr		23
pupae 2d postWPP		27
pupae 3d postWPP		29
pupae 4d postWPP		34
adult male 01day		56
adult male 05day		67
adult male 30day		73
adult female 01day		14
adult female 05day		17
adult female 30day		19
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		26
embryo 02-04hr		21
embryo 04-06hr		18
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		18
embryo 12-14hr		16
embryo 14-16hr		14
embryo 16-18hr		12
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		8
larva L1		10
larva L2		12
larva L3 12hr old		10
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		18
white prepupae new		19
white prepupae 12hr		19
white prepupae 24hr		23
pupae 2d postWPP		27
pupae 3d postWPP		29
pupae 4d postWPP		34
adult male 01day		56
adult male 05day		67
adult male 30day		73
adult female 01day		14
adult female 05day		17
adult female 30day		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		26
embryo 02-04hr		21
embryo 04-06hr		18
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		18
embryo 12-14hr		16
embryo 14-16hr		14
embryo 16-18hr		12
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		8
larva L1		10
larva L2		12
larva L3 12hr old		10
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		18
white prepupae new		19
white prepupae 12hr		19
white prepupae 24hr		23
pupae 2d postWPP		27
pupae 3d postWPP		29
pupae 4d postWPP		(34)
adult male 01day		(56)
adult male 05day		(67)
adult male 30day		(73)
adult female 01day		14
adult female 05day		17
adult female 30day		19
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		26
embryo 02-04hr		21
embryo 04-06hr		18
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		18
embryo 12-14hr		16
embryo 14-16hr		14
embryo 16-18hr		12
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		8
larva L1		10
larva L2		12
larva L3 12hr old		10
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		18
white prepupae new		19
white prepupae 12hr		19
white prepupae 24hr		23
pupae 2d postWPP		27
pupae 3d postWPP		29
pupae 4d postWPP		34
adult male 01day		56
adult male 05day		67
adult male 30day		73
adult female 01day		14
adult female 05day		17
adult female 30day		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		26
embryo 02-04hr		21
embryo 04-06hr		18
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		18
embryo 12-14hr		16
embryo 14-16hr		14
embryo 16-18hr		12
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		8
larva L1		10
larva L2		12
larva L3 12hr old		10
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		18
white prepupae new		19
white prepupae 12hr		19
white prepupae 24hr		23
pupae 2d postWPP		27
pupae 3d postWPP		29
pupae 4d postWPP		34
adult male 01day		56
adult male 05day		67
adult male 30day		73
adult female 01day		14
adult female 05day		17
adult female 30day		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		18
Schneider line 2 Sg4		13
embryonic 1182-4H		12
embryonic GM2		9
embryonic Kc167		7
embryonic S1		6
embryonic S3		7
leg disc CME L1		13
wing disc CME-W2		6
wing disc ML-DmD8		4
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		9
wing disc ML-DmD21		7
wing disc ML-DmD32		8
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		9
CNS ML-DmBG2-c2		13
tumorous blood cells mbn2		15
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		3
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		18
Schneider line 2 Sg4		13
embryonic 1182-4H		12
embryonic GM2		9
embryonic Kc167		7
embryonic S1		6
embryonic S3		7
leg disc CME L1		13
wing disc CME-W2		6
wing disc ML-DmD8		4
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		9
wing disc ML-DmD21		7
wing disc ML-DmD32		8
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		9
CNS ML-DmBG2-c2		13
tumorous blood cells mbn2		15
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		18
Schneider line 2 Sg4		13
embryonic 1182-4H		12
embryonic GM2		9
embryonic Kc167		7
embryonic S1		6
embryonic S3		7
leg disc CME L1		13
wing disc CME-W2		6
wing disc ML-DmD8		4
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		9
wing disc ML-DmD21		7
wing disc ML-DmD32		8
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		9
CNS ML-DmBG2-c2		13
tumorous blood cells mbn2		15
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		18
Schneider line 2 Sg4		13
embryonic 1182-4H		12
embryonic GM2		9
embryonic Kc167		7
embryonic S1		6
embryonic S3		7
leg disc CME L1		13
wing disc CME-W2		6
wing disc ML-DmD8		4
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		9
wing disc ML-DmD21		7
wing disc ML-DmD32		8
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		9
CNS ML-DmBG2-c2		13
tumorous blood cells mbn2		15
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		18
Schneider line 2 Sg4		13
embryonic 1182-4H		12
embryonic GM2		9
embryonic Kc167		7
embryonic S1		6
embryonic S3		7
leg disc CME L1		13
wing disc CME-W2		6
wing disc ML-DmD8		4
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		9
wing disc ML-DmD21		7
wing disc ML-DmD32		8
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		9
CNS ML-DmBG2-c2		13
tumorous blood cells mbn2		15
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		3
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		18
Schneider line 2 Sg4		13
embryonic 1182-4H		12
embryonic GM2		9
embryonic Kc167		7
embryonic S1		6
embryonic S3		7
leg disc CME L1		13
wing disc CME-W2		6
wing disc ML-DmD8		4
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		9
wing disc ML-DmD21		7
wing disc ML-DmD32		8
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		9
CNS ML-DmBG2-c2		13
tumorous blood cells mbn2		15
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		18
Schneider line 2 Sg4		13
embryonic 1182-4H		12
embryonic GM2		9
embryonic Kc167		7
embryonic S1		6
embryonic S3		7
leg disc CME L1		13
wing disc CME-W2		6
wing disc ML-DmD8		4
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		9
wing disc ML-DmD21		7
wing disc ML-DmD32		8
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		9
CNS ML-DmBG2-c2		13
tumorous blood cells mbn2		15
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		18
Schneider line 2 Sg4		13
embryonic 1182-4H		12
embryonic GM2		9
embryonic Kc167		7
embryonic S1		6
embryonic S3		7
leg disc CME L1		13
wing disc CME-W2		6
wing disc ML-DmD8		4
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		9
wing disc ML-DmD21		7
wing disc ML-DmD32		8
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		11
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		9
CNS ML-DmBG2-c2		13
tumorous blood cells mbn2		15
ovary fGS/OSS		4
ovary OSC		3
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		23
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		13
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		15
Ethanol 2.5% 3 hrs, larvae L3		27
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		18
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		24
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		23
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		13
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		15
Ethanol 2.5% 3 hrs, larvae L3		(27)
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		18
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		24
Paraquat 10 mM 48 hrs, 4-day adult		(29)
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		23
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		13
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		15
Ethanol 2.5% 3 hrs, larvae L3		27
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		18
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		24
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		23
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		13
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		15
Ethanol 2.5% 3 hrs, larvae L3		27
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		18
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		24
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		23
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		13
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		15
Ethanol 2.5% 3 hrs, larvae L3		27
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		18
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		24
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		23
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		13
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		15
Ethanol 2.5% 3 hrs, larvae L3		27
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		18
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		24
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		23
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		13
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		15
Ethanol 2.5% 3 hrs, larvae L3		27
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		18
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		24
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		4
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		23
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		13
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		15
Ethanol 2.5% 3 hrs, larvae L3		27
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		18
Caffeine 25 mg/ml 48 hrs, 4-day adult		9
Paraquat 5 mM 48 hrs, 4-day adult		24
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

LD30472

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0037900

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG5276-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

CG5276^GD2917

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of CG5276	Class	Mutagen	Stocks	Known lesion
CG5276^e03505		piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG5276	Class	Mutagen	Stocks	Known lesion
CG5276^GD2917		in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG5276^KK102311		in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes

Aneuploid Aberrations

Disrupted in

Df(3R)Exel6160

(Ryder, 2004.4.26)

Df(3R)Exel7308

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD2917}

P{KK102311}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}CG5276^e03505

Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )

Terms Based on Experimental Evidence ( 2 terms )

Molecular Function

CV term

Evidence

References

nucleoside-diphosphatase activity

inferred from direct assay

(Fenckova et al., 2011)

nucleoside-triphosphatase activity

inferred from direct assay

(Fenckova et al., 2011)

Biological Process ( 0 terms)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 3 terms )

Molecular Function

CV term

Evidence

References

calcium ion binding

inferred from electronic annotation with InterPro:IPR009283

(FlyBase Curators et al., 2004-)

nucleoside-diphosphatase activity

inferred from sequence or structural similarity with EMBL:AF085499

(FlyBase, 1992-)

nucleoside-triphosphatase activity

inferred from sequence or structural similarity with EMBL:AF085499

(FlyBase, 1992-)

Biological Process ( 0 terms)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG5276 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

66506

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0037900

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

41373

Orthologs

OrthoDB Orthologs (41) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6575SG

Dipteran inclusive ortholog search

EOG6M65S2

Insect inclusive ortholog search

EOG6JM6K4

Arthropod inclusive ortholog search

EOG6JH9X4

Metazoa inclusive ortholog search

EOG6TR04C

Orthologs in Drosophila Species (EOG6575SG)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18779

Drosophila persimilis

Dper\GL12374

Drosophila willistoni

Dwil\GK11295

Drosophila virilis

Dvir\GJ11094

Drosophila mojavensis

Dmoj\GI10286

Drosophila grimshawi

Dgri\GH17322

Orthologs in non-Drosophila Dipterans (EOG6M65S2)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006659

Anopheles gambiae

Malaria mosquito

Agam\AGAP001217

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ006723

Orthologs in non-Dipteran Insects (EOG6JM6K4)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB55053

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007666

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH19985

Atta cephalotes

Leafcutter ant

Acep\ACEP24694

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO18493

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL12775

Linepithema humile

Argentine ant

Lhum\LH24688

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB16148

Solenopsis invicta

Red fire ant

Sinv\SINV23174

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004254

Bombyx mori

Silkmoth

Bmor\BGIBMGA007005

Pediculus humanus

Human body louse

Phum\PHUM421050

Tribolium castaneum

Red flour beetle

Tcas\TC005356

Orthologs in non-Insect Arthropods (EOG6JH9X4)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_188560

Ixodes scapularis

Deer tick

Isca\ISCW018254

Orthologs in non-Arthropod Metazoa (EOG6TR04C)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\ZK563.7

Caenorhabditis elegans

Nematode

Cele\apy-1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_001924gn

Danio rerio

Zebrafish

Drer\cant1b

Danio rerio

Zebrafish

Drer\ENSDARG00000016398

Danio rerio

Zebrafish

Drer\cant1a

Xenopus tropicalis

Western clawed frog

Xtro\cant1

Gallus gallus

Domestic chicken

Ggal\CANT1

Mus musculus

House mouse

Mmus\Cant1

Rattus norvegicus

Norway rat

Rnor\Cant1

Homo sapiens

Human

Hsap\CANT1

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\CANT1

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18779

Drosophila persimilis

Dper\GL12374

Drosophila willistoni

Dwil\GK11295

Drosophila virilis

Dvir\GJ11094

Drosophila mojavensis

Dmoj\GI10286

Drosophila grimshawi

Dgri\GH17322

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Bloomington

18162

w¹¹¹⁸; PBac{RB}CG5276^e03505/TM6B, Tb¹

Harvard

PBac{RB}CG5276^e03505

VDRC

v106691

P{KK102311}VIE-260B

v47995

w¹¹¹⁸; P{GD2917}v47995/TM3

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 70 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD30472

Other clones

DPiM_LD30472

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RP003006585
RP003019373
RP003022311
RP003025554
RP003032138
RP003033304
RP003041991
RP003049329
RP003068884
RP003076628
RP003096480
RP003099207
RP003104489
RP003110344
RP003115473
RP003122181
RP003144113
RP003155310
RP003174700
RP003177859
RP003187901
RP003189557
RP003191447
RP003198618
RP003207949
RP003238745
RP003261178
RP003263169
RP003297982
RP003319129
RP003319674
RP003335191
RP003343290
RP003344701
RP003354621
RP003359411
RP003374463
RP003381869
RP003385544
RP003396085
RP003397078
RP003397776
RP003404226
RP003405938
RP003413602
RP003424969
RP003429372
RP003433181
RP003441937
RP003442890
RP003458031
RP003473450
RP003489991
RP003499842
RP003509936
RP003530763
RP003558694
RP003563385
RP003645036

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0037900

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

41373

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_141817

RefSeq (Proteins)

•

NP_650074

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

41373

UniProtKB/TrEMBL

•

Q9VGN8

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Apyrase (IPR009283)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

LD30472

Linkouts

BioGRID - A database of protein and genetic interactions

•

66506

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0037900

DRSC - Results from RNAi screens.

•

FBgn0037900

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0037900

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG5276-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0037900

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

41373

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

41373

modMine - Data generated by the modENCODE project.

•

FBgn0037900

Synonyms & Secondary IDs ( 1 )

Reported As

Symbol Synonym

CG5276

(Svensson et al., 2007, Fenckova et al., 2011, Keleman et al., 2009.8.5)

Name Synonym

Secondary FlyBase IDs

References ( 18 )

Research paper

Fenckova et al., 2011, Insect Biochem. Mol. Biol. 41(12): 956--967: Functional characterization of ecto-5'-nucleotidases and apyrases in Drosophila melanogaster. [FBrf0216637]
Svensson et al., 2007, Dev. Genes Evol. 217(9): 639--650: Organization and regulation of sex-specific thioredoxin encoding genes in the genus Drosophila. [FBrf0201263]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file): Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.: Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
Roth and Foulger, 2002.4.24, Predicted GO terms.: Predicted GO terms. [FBrf0146674]
Gene Disruption Project members, 2001-, (Computer file): (Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

FlyBase analysis

FlyBase, 1992-, FlyBase curation.: FlyBase curation. [FBrf0105495]

Curated genome annotation

Ashburner, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126651]