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Gene Dmel\HisCl1

General Information
Symbol	Dmel\HisCl1	Species	D. melanogaster
Name	Histamine-gated chloride channel subunit 1	Annotation symbol	CG14723
Feature type	protein_coding_gene	FlyBase ID	FBgn0037950
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	hclB
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	86F7-86F8	Sequence location	3R:7,612,898..7,615,115 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Histamine-gated chloride channel subunit 1 is referred to in FlyBase by the symbol Dmel\HisCl1 (CG14723, FBgn0037950). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: extracellular-glycine-gated ion channel activity. There is experimental evidence that it is involved in the biological process: thermotaxis. 12 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Gamma-aminobutyric acid A receptor; Neurotransmitter-gated ion-channel; Neurotransmitter-gated ion-channel ligand-binding; Neurotransmitter-gated ion-channel transmembrane domain; Neurotransmitter-gated ion-channel, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of low expression to a trough of extremely low expression. Peak expression observed at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye. Comments on Affy2 ProbeSet: ProbeSet 1626897_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of HisCl1. Gene sequence location is 3R:7612898..7615115.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Controlled Vocabulary Terms P-stage pharate adult stage P15 pharate adult stage P12(ii) pharate adult stage P9 organism adult abdomen pharate adult stage P10 pharate adult stage P14 pharate adult stage P15(ii) adult thorax lamina faint pharate adult stage P13 pharate adult stage P11 pharate adult stage P8 pupal stage embryonic stage pharate adult stage prepupal stage pharate adult stage P12 pharate adult stage P15(i) pharate adult stage P12(i) dorsomedial neurosecretory cell pharate adult stage P11(i) pharate adult stage P11(ii) adult head Unknown in situ Sequence features BKN32578 HFA14945
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 86F7-86F8   Limits computationally determined from genome sequence between P{EP}Lk6EP886 and P{PZ}svp07842
Experimentally Determined Cytological Location
Cytogenetic map Notes References 86F-86F     (Zheng, 2001.5.22)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\HisCl1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone GH14445 appears problematic: unspliced intron
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) HisCl1-RA FBtr0082470  NM_169429   1758   426 HisCl1-RB FBtr0082471  NM_141859   1711   422 HisCl1-RC FBtr0082472  NM_206474   1661   233 HisCl1-RD FBtr0301826  NM_001170120   1657   416
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein HisCl1-PA   FBpp0081946   49.3   426   6.22     NP_731632   AAF54699 HisCl1-PB   FBpp0089166   48.9   422   6.22     NP_650116   AAN13530 HisCl1-PC   FBpp0089167   27.1   233   7.34     NP_996196   AAS65139 HisCl1-PD   FBpp0291040   48.3   416   6.36     NP_001163591   ACZ94888
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Neurotransmitter-gated ion-channel, conserved site (IPR018000) Gamma-aminobutyric acid A receptor (IPR006028) Neurotransmitter-gated ion-channel transmembrane domain (IPR006029) Neurotransmitter-gated ion-channel (IPR006201) Neurotransmitter-gated ion-channel ligand-binding (IPR006202)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF54699   AE014297 AAN13530   AE014297 AAS65139   AE014297 ACZ94888   AF382401 AAL66186   AF382402 AAL66187   AF435470 AAL74414   AF435471 AAL74415   AF467953 AAL76078   AI238407   AW940548   AX254937 CAC94524   AX254939 CAC94525   AX254941 CAC94526   AY049775 AAL12211   AY060303 AAL25342   CO320519   EC090080
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8T9R9 Q8WS32 Q95T69 Q9VGI0 E1JII9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
expression microarray Stage Tissue/Position (including subcellular localization) Reference adult stage   eye   (Xu et al., 2004) in situ Stage Tissue/Position (including subcellular localization) Reference adult stage   lamina   (Gisselmann et al., 2002) dorsomedial neurosecretory cell   (Gisselmann et al., 2002) RT-PCR Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism   (Gisselmann et al., 2002) prepupal stage   organism | faint   (Gisselmann et al., 2002) pupal stage -- adult stage   organism   (Gisselmann et al., 2002) adult stage   adult head   (Gisselmann et al., 2002) adult thorax & adult abdomen   (Gisselmann et al., 2002) Comment:reference states "adult body"
Additional Descriptive Data
	HisCl1 transcript cannot be detected in eggs or larvae, and is only faintly detectable in prepupae, using RT-PCR. Expression is detected in both young and old adults. (Gisselmann et al., 2002) Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads. (Xu et al., 2004)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference adult stage   adult pars intercerebralis | restricted   (Hong et al., 2006) adult subesophageal ganglion | restricted   (Hong et al., 2006) adult protocerebrum | lateral & restricted   (Hong et al., 2006) adult DN period neuron | lateral & restricted   (Hong et al., 2006) thoracico-abdominal ganglion | restricted   (Hong et al., 2006) fan-shaped body | restricted   (Hong et al., 2006) medulla | restricted   (Hong et al., 2006) LNv neuron | subset   (Hong et al., 2006) Comment:Expressed in 4 cells.
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0037950    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0037950 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of low expression to a trough of extremely low expression. Peak expression observed at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0037950 expression level Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    72 embryo 04-06hr    54 embryo 06-08hr    25 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    24 embryo 18-20hr    14 embryo 20-22hr    5 embryo 22-24hr    12 larva L1    12 larva L2    9 larva L3 12hr old    3 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    44 larva L3 puffstage 7-9    91 white prepupae new    178 white prepupae 12hr    88 white prepupae 24hr    110 pupae 2d postWPP    189 pupae 3d postWPP    270 pupae 4d postWPP    178 adult male 01day    184 adult male 05day    207 adult male 30day    189 adult female 01day    69 adult female 05day    26 adult female 30day    37 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    72 embryo 04-06hr    54 embryo 06-08hr    25 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    24 embryo 18-20hr    14 embryo 20-22hr    5 embryo 22-24hr    12 larva L1    12 larva L2    9 larva L3 12hr old    3 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    44 larva L3 puffstage 7-9    91 white prepupae new  (178) white prepupae 12hr    88 white prepupae 24hr  (110) pupae 2d postWPP  (189) pupae 3d postWPP  (270) pupae 4d postWPP  (178) adult male 01day  (184) adult male 05day  (207) adult male 30day  (189) adult female 01day    69 adult female 05day    26 adult female 30day    37 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    72 embryo 04-06hr    54 embryo 06-08hr    25 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    24 embryo 18-20hr    14 embryo 20-22hr    5 embryo 22-24hr    12 larva L1    12 larva L2    9 larva L3 12hr old    3 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    44 larva L3 puffstage 7-9    91 white prepupae new    178 white prepupae 12hr    88 white prepupae 24hr    110 pupae 2d postWPP    189 pupae 3d postWPP    270 pupae 4d postWPP    178 adult male 01day    184 adult male 05day    207 adult male 30day    189 adult female 01day    69 adult female 05day    26 adult female 30day    37 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    72 embryo 04-06hr    54 embryo 06-08hr    25 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    24 embryo 18-20hr    14 embryo 20-22hr    5 embryo 22-24hr    12 larva L1    12 larva L2    9 larva L3 12hr old    3 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    44 larva L3 puffstage 7-9    91 white prepupae new    178 white prepupae 12hr    88 white prepupae 24hr    110 pupae 2d postWPP    189 pupae 3d postWPP    270 pupae 4d postWPP    178 adult male 01day    184 adult male 05day    207 adult male 30day    189 adult female 01day    69 adult female 05day    26 adult female 30day    37 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    72 embryo 04-06hr    54 embryo 06-08hr    25 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    24 embryo 18-20hr    14 embryo 20-22hr    5 embryo 22-24hr    12 larva L1    12 larva L2    9 larva L3 12hr old    3 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    44 larva L3 puffstage 7-9    91 white prepupae new    178 white prepupae 12hr    88 white prepupae 24hr    110 pupae 2d postWPP    189 pupae 3d postWPP    270 pupae 4d postWPP    178 adult male 01day    184 adult male 05day    207 adult male 30day    189 adult female 01day    69 adult female 05day    26 adult female 30day    37 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0037950 expression level Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    72 embryo 04-06hr    54 embryo 06-08hr    25 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    24 embryo 18-20hr    14 embryo 20-22hr    5 embryo 22-24hr    12 larva L1    12 larva L2    9 larva L3 12hr old    3 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    44 larva L3 puffstage 7-9    91 white prepupae new    178 white prepupae 12hr    88 white prepupae 24hr    110 pupae 2d postWPP    189 pupae 3d postWPP    270 pupae 4d postWPP    178 adult male 01day    184 adult male 05day    207 adult male 30day    189 adult female 01day    69 adult female 05day    26 adult female 30day    37 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    72 embryo 04-06hr    54 embryo 06-08hr    25 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    24 embryo 18-20hr    14 embryo 20-22hr    5 embryo 22-24hr    12 larva L1    12 larva L2    9 larva L3 12hr old    3 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    44 larva L3 puffstage 7-9    91 white prepupae new  (178) white prepupae 12hr    88 white prepupae 24hr  110 pupae 2d postWPP  (189) pupae 3d postWPP  (270) pupae 4d postWPP  (178) adult male 01day  (184) adult male 05day  (207) adult male 30day  (189) adult female 01day    69 adult female 05day    26 adult female 30day    37 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    72 embryo 04-06hr    54 embryo 06-08hr    25 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    24 embryo 18-20hr    14 embryo 20-22hr    5 embryo 22-24hr    12 larva L1    12 larva L2    9 larva L3 12hr old    3 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    44 larva L3 puffstage 7-9    91 white prepupae new    178 white prepupae 12hr    88 white prepupae 24hr    110 pupae 2d postWPP    189 pupae 3d postWPP    270 pupae 4d postWPP    178 adult male 01day    184 adult male 05day    207 adult male 30day    189 adult female 01day    69 adult female 05day    26 adult female 30day    37 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    72 embryo 04-06hr    54 embryo 06-08hr    25 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    24 embryo 18-20hr    14 embryo 20-22hr    5 embryo 22-24hr    12 larva L1    12 larva L2    9 larva L3 12hr old    3 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    44 larva L3 puffstage 7-9    91 white prepupae new    178 white prepupae 12hr    88 white prepupae 24hr    110 pupae 2d postWPP    189 pupae 3d postWPP    270 pupae 4d postWPP    178 adult male 01day    184 adult male 05day    207 adult male 30day    189 adult female 01day    69 adult female 05day    26 adult female 30day    37 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    72 embryo 04-06hr    54 embryo 06-08hr    25 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    24 embryo 18-20hr    14 embryo 20-22hr    5 embryo 22-24hr    12 larva L1    12 larva L2    9 larva L3 12hr old    3 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    44 larva L3 puffstage 7-9    91 white prepupae new    178 white prepupae 12hr    88 white prepupae 24hr    110 pupae 2d postWPP    189 pupae 3d postWPP    270 pupae 4d postWPP    178 adult male 01day    184 adult male 05day    207 adult male 30day    189 adult female 01day    69 adult female 05day    26 adult female 30day    37 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0037950    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0037950 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0037950 expression level Tissue   Expression Level Larval Central Nervous System    9.7 Larval Midgut    6.6 Larval Hindgut    4.3 Larval Malpighian Tubules    4.4 Larval Fat Body no informative data Larval Salivary Gland    5.6 Larval Trachea    4.075 Larval Carcass    5.15 Adult Head    62.2 Adult Eye    217 Adult Brain    30.6 Adult Thoracic-Abdominal Ganglion    35.3 Adult Crop    4.9 Adult Midgut    4 Adult Hindgut    7.3 Adult Malpighian Tubules    6.1 Adult Fat Body    4.2 Adult Salivary Gland no informative data Adult Heart    5.425 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.6 Adult Ovary    3.6 Adult Testis no informative data Adult Male Accessory Gland    67.6 Adult Carcass    3.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    9.7 Larval Midgut    6.6 Larval Hindgut    4.3 Larval Malpighian Tubules    4.4 Larval Fat Body no informative data Larval Salivary Gland    5.6 Larval Trachea    4.075 Larval Carcass    5.15 Adult Head    62.2 Adult Eye    217 Adult Brain    30.6 Adult Thoracic-Abdominal Ganglion    35.3 Adult Crop    4.9 Adult Midgut    4 Adult Hindgut    7.3 Adult Malpighian Tubules    6.1 Adult Fat Body    4.2 Adult Salivary Gland no informative data Adult Heart    5.425 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.6 Adult Ovary    3.6 Adult Testis no informative data Adult Male Accessory Gland    67.6 Adult Carcass    3.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    9.7 Larval Midgut    6.6 Larval Hindgut    4.3 Larval Malpighian Tubules    4.4 Larval Fat Body no informative data Larval Salivary Gland    5.6 Larval Trachea    4.075 Larval Carcass    5.15 Adult Head    62.2 Adult Eye    217 Adult Brain    30.6 Adult Thoracic-Abdominal Ganglion    35.3 Adult Crop    4.9 Adult Midgut    4 Adult Hindgut    7.3 Adult Malpighian Tubules    6.1 Adult Fat Body    4.2 Adult Salivary Gland no informative data Adult Heart    5.425 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.6 Adult Ovary    3.6 Adult Testis no informative data Adult Male Accessory Gland    67.6 Adult Carcass    3.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    9.7 Larval Midgut    6.6 Larval Hindgut    4.3 Larval Malpighian Tubules    4.4 Larval Fat Body no informative data Larval Salivary Gland    5.6 Larval Trachea    4.075 Larval Carcass    5.15 Adult Head    62.2 Adult Eye    217 Adult Brain    30.6 Adult Thoracic-Abdominal Ganglion    35.3 Adult Crop    4.9 Adult Midgut    4 Adult Hindgut    7.3 Adult Malpighian Tubules    6.1 Adult Fat Body    4.2 Adult Salivary Gland no informative data Adult Heart    5.425 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.6 Adult Ovary    3.6 Adult Testis no informative data Adult Male Accessory Gland    67.6 Adult Carcass    3.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0037950 expression level Tissue   Expression Level Larval Central Nervous System    9.7 Larval Midgut    6.6 Larval Hindgut    4.3 Larval Malpighian Tubules    4.4 Larval Fat Body no informative data Larval Salivary Gland    5.6 Larval Trachea    4.075 Larval Carcass    5.15 Adult Head    62.2 Adult Eye    217 Adult Brain    30.6 Adult Thoracic-Abdominal Ganglion    35.3 Adult Crop    4.9 Adult Midgut    4 Adult Hindgut    7.3 Adult Malpighian Tubules    6.1 Adult Fat Body    4.2 Adult Salivary Gland no informative data Adult Heart    5.425 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.6 Adult Ovary    3.6 Adult Testis no informative data Adult Male Accessory Gland    67.6 Adult Carcass    3.5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    9.7 Larval Midgut    6.6 Larval Hindgut    4.3 Larval Malpighian Tubules    4.4 Larval Fat Body no informative data Larval Salivary Gland    5.6 Larval Trachea    4.075 Larval Carcass    5.15 Adult Head    62.2 Adult Eye    217 Adult Brain    30.6 Adult Thoracic-Abdominal Ganglion    35.3 Adult Crop    4.9 Adult Midgut    4 Adult Hindgut    7.3 Adult Malpighian Tubules    6.1 Adult Fat Body    4.2 Adult Salivary Gland no informative data Adult Heart    5.425 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.6 Adult Ovary    3.6 Adult Testis no informative data Adult Male Accessory Gland    67.6 Adult Carcass    3.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    9.7 Larval Midgut    6.6 Larval Hindgut    4.3 Larval Malpighian Tubules    4.4 Larval Fat Body no informative data Larval Salivary Gland    5.6 Larval Trachea    4.075 Larval Carcass    5.15 Adult Head    62.2 Adult Eye    217 Adult Brain    30.6 Adult Thoracic-Abdominal Ganglion    35.3 Adult Crop    4.9 Adult Midgut    4 Adult Hindgut    7.3 Adult Malpighian Tubules    6.1 Adult Fat Body    4.2 Adult Salivary Gland no informative data Adult Heart    5.425 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.6 Adult Ovary    3.6 Adult Testis no informative data Adult Male Accessory Gland    67.6 Adult Carcass    3.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    9.7 Larval Midgut    6.6 Larval Hindgut    4.3 Larval Malpighian Tubules    4.4 Larval Fat Body no informative data Larval Salivary Gland    5.6 Larval Trachea    4.075 Larval Carcass    5.15 Adult Head    62.2 Adult Eye    217 Adult Brain    30.6 Adult Thoracic-Abdominal Ganglion    35.3 Adult Crop    4.9 Adult Midgut    4 Adult Hindgut    7.3 Adult Malpighian Tubules    6.1 Adult Fat Body    4.2 Adult Salivary Gland no informative data Adult Heart    5.425 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.6 Adult Ovary    3.6 Adult Testis no informative data Adult Male Accessory Gland    67.6 Adult Carcass    3.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis no informative data Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    62.2   NA  Eye    217   NA  Brain    30.6   9.7  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    35.3   NA  Crop    4.9   6.6  Midgut    4   4.3  Hindgut    7.3   4.4  Malpighian Tubules    6.1   no informative data  Fat Body    4.2   5.6  Salivary Gland    no informative data   NA  Heart    5.425   4.075  Trachea    NA   NA  VirginFemale Spermatheca    3.9   NA  InseminatedFemale Spermatheca    3.6   NA  Ovary    3.6   NA  Testis    no informative data   NA  Male Accessory Gland    67.6   5.15  Carcass    3.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_28
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0037950 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14723-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 HisCl1GD2925 viable HisCl1EP73547 Other Phenotypes Allele chemical resistant (with Df(3R)E79) HisCl1T2 chemical sensitive (with Df(3R)E79) HisCl1T1 HisCl1T2 neurophysiology defective (with Df(3R)E79) HisCl1T1 HisCl1T2 paralytic | heat sensitive (with Df(3R)E79) HisCl1T1 HisCl1T2 paralytic | recessive | heat sensitive HisCl1134 thermotaxis behavior defective | recessive HisCl1134 HisCl1EP73547 Phenotype manifest in Allele
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of HisCl1 Class Mutagen Stocks Known lesion HisCl1T2 ethyl methanesulfonate 2 Yes HisCl1G4549 P-element activity 1 -- HisCl1T1 ethyl methanesulfonate 1 Yes HisCl1W282stop ethyl methanesulfonate 1 Yes HisCl1W286C ethyl methanesulfonate 1 Yes HisCl1134 Delta2-3 0 Yes HisCl1384 P-element activity 0 Yes HisCl1EP73547 hypomorphic allele - genetic evidence 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of HisCl1 Class Mutagen Stocks Known lesion HisCl1GD2925 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes HisCl1JF02847 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes HisCl1KK112578 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes HisCl1+t14.3 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel7310 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD2925} NA P{KK112578} NA P{TRiP.JF02847} NA characterization construct P{HisCl1+t14.3} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}HisCl1EP73547 NA P{EP}HisCl1G4549 NA P{GSV1}C547C547 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
thermotaxis inferred from mutant phenotype (Hong et al., 2006)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
extracellular-glycine-gated ion channel activity inferred from sequence or structural similarity with UniProtKB:Q61603 (FlyBase, 1992-) histamine-gated chloride channel activity non-traceable author statement (Zheng, 2001.5.22, Yin, 2001.8.2)
Biological Process
CV term Evidence References
ion transport inferred from electronic annotation with InterPro:IPR006028, InterPro:IPR006029, InterPro:IPR006201, InterPro:IPR018000 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
histamine-gated chloride channel complex non-traceable author statement (Zheng, 2001.5.22, Yin, 2001.8.2) integral to membrane inferred from electronic annotation with InterPro:IPR006028, InterPro:IPR006201, InterPro:IPR018000 (FlyBase Curators et al., 2004-) postsynaptic membrane inferred from electronic annotation with InterPro:IPR018000 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions HisCl1 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0037950 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41426
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18400 Dere\GG18454 Dgri\GH17342 Dmoj\GI22447 Dper\GL12390 Dpse\GA13205 Dsec\GM23979 Dsim\GD18784 Dvir\GJ11009 Dwil\GK12944 Dyak\GE24203
Curated drosophilid orthologs	Dpse\GA13205
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • XP_624072.1 Apis mellifera (honey bee) XP_001946739.1 Acyrthosiphon pisum (pea aphid) XP_001900888.1 Brugia malayi (roundworm nematode) 165308 Capitella spl (polychaete worm) 300576 Daphnia pulex (daphnia) Smp_096480 Schistosoma mansoni (trematode parasite) NP_001103421.1 Tribolium castaneum (red flour beetle) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB17443 NV50171 LH13060 FBgn0241334 FBgn0214953 FBgn0198276 FBgn0190303 FBgn0178844 JGI_V11_328373 JGI_V11_300576 FBgn0073242 FBgn0149997 FBgn0149862 FBgn0145175 FBgn0124812 FBgn0110668 FBgn0095418 BGIBMGA005723 ACYPI007978 GB15968 GLEAN_04608
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	29632 st1 HisCl1T2 29634 st1 HisCl1W282stop 31787 w1118; P{EP}HisCl1G4549 29635 st1 HisCl1T2 es 28013 y1 v1; P{TRiP.JF02847}attP2 29631 st1 HisCl1T1/TM3, Sb1 29633 st1 HisCl1W286C/TM3, Sb1
VDRC	v104966 P{KK112578}VIE-260B v6065 w1118; P{GD2925}v6065
Genomic Clones ( 2 )
	BACR03D22 BACR11G22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 3 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH14445
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	180808 EK285932
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0037950 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41426
Antibody Information
	polyclonal (Hong et al., 2006)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: HisCl1 CG14723 (FlyBase, 1996-)
Source for database merge of
Additional comments	Source for identity of HisCl1 CG14723 was sequence comparison (date:020123). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_141859 NM_169429 NM_206474 NM_001170120 RefSeq (Proteins) • NP_650116 NP_731632 NP_996196 NP_001163591 DDBJ / EMBL / GenBank • AAF54699 AAL12211 AAL25342 AAL66186 AAL66187 AAL74414 AAL74415 AAL76078 AAN13530 AAS65139 ACZ94888 AF382401 AF382402 AF435470 AF435471 AF467953 AI238407 AW940548 AX254937 AX254939 AX254941 AY049775 AY060303 CAC94524 CAC94525 CAC94526 Entrez Gene - A searchable database of RefSeq genes. • 41426 UniProtKB/TrEMBL • E1JII9 Q8T9R9 Q8WS32 Q9VGI0 Q95T69
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Neurotransmitter-gated ion-channel, conserved site (IPR018000) Gamma-aminobutyric acid A receptor (IPR006028) Neurotransmitter-gated ion-channel transmembrane domain (IPR006029) Neurotransmitter-gated ion-channel (IPR006201) Neurotransmitter-gated ion-channel ligand-binding (IPR006202)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0037950 DRSC - Results from RNAi screens. • FBgn0037950 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0037950 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14723-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0037950 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41426 InParanoid A subset of ortholog calls from InParanoid. • XP_624072.1 Apis mellifera (honey bee) XP_001946739.1 Acyrthosiphon pisum (pea aphid) XP_001900888.1 Brugia malayi (roundworm nematode) 165308 Capitella spl (polychaete worm) 300576 Daphnia pulex (daphnia) Smp_096480 Schistosoma mansoni (trematode parasite) NP_001103421.1 Tribolium castaneum (red flour beetle) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41426 modMine - Data generated by the modENCODE project. • FBgn0037950 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB17443 NV50171 LH13060 FBgn0241334 FBgn0214953 FBgn0198276 FBgn0190303 FBgn0178844 JGI_V11_328373 JGI_V11_300576 FBgn0073242 FBgn0149997 FBgn0149862 FBgn0145175 FBgn0124812 FBgn0110668 FBgn0095418 BGIBMGA005723 ACYPI007978 GB15968 GLEAN_04608
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG14723 (Xu et al., 2004, Gengs et al., 2002, Molnar et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009) Dm-HCLB (Iovchev et al., 2006) DmHisCl1 (Zheng et al., 2002) DMHisClα2 (Gisselmann et al., 2002) hclB (Iovchev et al., 2002, Gengs et al., 2002, Yin, 2001.8.2, Semenov et al., 2007, Pantazis et al., 2008, Yusein et al., 2008, Yusein et al., 2010, Yusein et al., 2008) hisCl1 (Zheng et al., 2002, Hong et al., 2006) HisCl1 (Gao et al., 2008, Molnar et al., 2006, Yusein-Myashkova and Semenov, 2009.12.3, Gao et al., 2008)
Name Synonym	Histamine-gated chloride channel subunit 1   histamine-gated chloride channel subunit 1 (Hong et al., 2006)
Secondary FlyBase IDs
References ( 38 )
Generate a list of	All references relating to this gene ( 38 )
List References by type	Research paper  ( 13 ) Supplementary material  ( 3 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 7 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Patent  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 2 )
	Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784 GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] Yusein et al., 2010, J. Insect Physiol. 56(1): 21--27 Functional consequences of mutations in the Drosophila histamine receptor HCLB. [FBrf0209538] Show all research papers ...