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Gene Dmel\CG8449

General Information
Symbol	Dmel\CG8449	Species	D. melanogaster
Name		Annotation symbol	CG8449
Feature type	protein_coding_gene	FlyBase ID	FBgn0038129
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	87E3-87E3	Sequence location	3R:9,094,859..9,097,733 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG8449 (FBgn0038129). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: Rab GTPase activator activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: regulation of Rab GTPase activity. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Rab-GTPase-TBC domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval/adult fat body, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval hindgut, larval Malpighian tubules, adult heart, larval salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1633082_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG8449. Gene sequence location is 3R:9094859..9097733.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 87E3-87E3   Limits computationally determined from genome sequence between P{PZ}CtBP03463 and P{lacW}B52s2249&P{lacW}flflL4179
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG8449 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47 Low-frequency RNA-Seq exon junction(s) not annotated. DGC clone LD09351 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG8449-RA FBtr0082778  NM_169499   2318   654
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG8449-PA   FBpp0082246   74.3   654   6.37     NP_731780   AAF54919
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Rab-GTPase-TBC domain (IPR000195)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390390   AA694688   AA817079   AA821008   AA940741   AA941104   AA951472   AE014297 AAF54919   AI108672   AI108754   AI258569   AI389019   AI456348   AI512842   AI514626   AI519604   AI533842   AI543318   AW942071   AY061105 AAL28653   BH840735   BI163173   BI352184   BI363608   BI604490   BI622630   BI636090   BI637327   BP540813   BP540814   BT023950   CO156408   CO177013   CO286989   CO303780   CO329141   CO337775   EC198732   EC206096   EC214724   EC230123   EC243623   EC248174   EC258199   EC262134   EC265240   EV584952
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VFX6 Q95RV7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval/adult fat body, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval hindgut, larval Malpighian tubules, adult heart, larval salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    118.425 Larval Midgut    67.6 Larval Hindgut    109.4 Larval Malpighian Tubules    142.2 Larval Fat Body    1877.2 Larval Salivary Gland    115.3 Larval Trachea    151.625 Larval Carcass    105.05 Adult Head    249.3 Adult Eye    189.775 Adult Brain    118.3 Adult Thoracic-Abdominal Ganglion    123.2 Adult Crop    128.6 Adult Midgut    49.5 Adult Hindgut    91.9 Adult Malpighian Tubules    86.3 Adult Fat Body    744.2 Adult Salivary Gland    75.9 Adult Heart    398.625 Adult VirginFemale Spermatheca    944.1 Adult InseminatedFemale Spermatheca    825.3 Adult Ovary    151.4 Adult Testis    32.4 Adult Male Accessory Gland    144.1 Adult Carcass    328.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    118.425 Larval Midgut    67.6 Larval Hindgut    109.4 Larval Malpighian Tubules    142.2 Larval Fat Body  (1877.2) Larval Salivary Gland    115.3 Larval Trachea    151.625 Larval Carcass    105.05 Adult Head    249.3 Adult Eye    189.775 Adult Brain    118.3 Adult Thoracic-Abdominal Ganglion    123.2 Adult Crop    128.6 Adult Midgut    49.5 Adult Hindgut    91.9 Adult Malpighian Tubules    86.3 Adult Fat Body  (744.2) Adult Salivary Gland    75.9 Adult Heart    398.625 Adult VirginFemale Spermatheca  (944.1) Adult InseminatedFemale Spermatheca  (825.3) Adult Ovary    151.4 Adult Testis    32.4 Adult Male Accessory Gland    144.1 Adult Carcass    328.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    118.425 Larval Midgut    67.6 Larval Hindgut    109.4 Larval Malpighian Tubules    142.2 Larval Fat Body  (1877.2) Larval Salivary Gland    115.3 Larval Trachea    151.625 Larval Carcass    105.05 Adult Head    249.3 Adult Eye    189.775 Adult Brain    118.3 Adult Thoracic-Abdominal Ganglion    123.2 Adult Crop    128.6 Adult Midgut    49.5 Adult Hindgut    91.9 Adult Malpighian Tubules    86.3 Adult Fat Body    744.2 Adult Salivary Gland    75.9 Adult Heart    398.625 Adult VirginFemale Spermatheca    944.1 Adult InseminatedFemale Spermatheca    825.3 Adult Ovary    151.4 Adult Testis    32.4 Adult Male Accessory Gland    144.1 Adult Carcass    328.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    118.425 Larval Midgut    67.6 Larval Hindgut    109.4 Larval Malpighian Tubules    142.2 Larval Fat Body    1877.2 Larval Salivary Gland    115.3 Larval Trachea    151.625 Larval Carcass    105.05 Adult Head    249.3 Adult Eye    189.775 Adult Brain    118.3 Adult Thoracic-Abdominal Ganglion    123.2 Adult Crop    128.6 Adult Midgut    49.5 Adult Hindgut    91.9 Adult Malpighian Tubules    86.3 Adult Fat Body    744.2 Adult Salivary Gland    75.9 Adult Heart    398.625 Adult VirginFemale Spermatheca    944.1 Adult InseminatedFemale Spermatheca    825.3 Adult Ovary    151.4 Adult Testis    32.4 Adult Male Accessory Gland    144.1 Adult Carcass    328.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    118.425 Larval Midgut    67.6 Larval Hindgut    109.4 Larval Malpighian Tubules    142.2 Larval Fat Body    1877.2 Larval Salivary Gland    115.3 Larval Trachea    151.625 Larval Carcass    105.05 Adult Head    249.3 Adult Eye    189.775 Adult Brain    118.3 Adult Thoracic-Abdominal Ganglion    123.2 Adult Crop    128.6 Adult Midgut    49.5 Adult Hindgut    91.9 Adult Malpighian Tubules    86.3 Adult Fat Body    744.2 Adult Salivary Gland    75.9 Adult Heart    398.625 Adult VirginFemale Spermatheca    944.1 Adult InseminatedFemale Spermatheca    825.3 Adult Ovary    151.4 Adult Testis    32.4 Adult Male Accessory Gland    144.1 Adult Carcass    328.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    118.425 Larval Midgut    67.6 Larval Hindgut    109.4 Larval Malpighian Tubules    142.2 Larval Fat Body  (1877.2) Larval Salivary Gland    115.3 Larval Trachea    151.625 Larval Carcass    105.05 Adult Head    249.3 Adult Eye    189.775 Adult Brain    118.3 Adult Thoracic-Abdominal Ganglion    123.2 Adult Crop    128.6 Adult Midgut    49.5 Adult Hindgut    91.9 Adult Malpighian Tubules    86.3 Adult Fat Body  744.2 Adult Salivary Gland    75.9 Adult Heart    398.625 Adult VirginFemale Spermatheca  (944.1) Adult InseminatedFemale Spermatheca  (825.3) Adult Ovary    151.4 Adult Testis    32.4 Adult Male Accessory Gland    144.1 Adult Carcass    328.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    118.425 Larval Midgut    67.6 Larval Hindgut    109.4 Larval Malpighian Tubules    142.2 Larval Fat Body  (1877.2) Larval Salivary Gland    115.3 Larval Trachea    151.625 Larval Carcass    105.05 Adult Head    249.3 Adult Eye    189.775 Adult Brain    118.3 Adult Thoracic-Abdominal Ganglion    123.2 Adult Crop    128.6 Adult Midgut    49.5 Adult Hindgut    91.9 Adult Malpighian Tubules    86.3 Adult Fat Body    744.2 Adult Salivary Gland    75.9 Adult Heart    398.625 Adult VirginFemale Spermatheca    944.1 Adult InseminatedFemale Spermatheca    825.3 Adult Ovary    151.4 Adult Testis    32.4 Adult Male Accessory Gland    144.1 Adult Carcass    328.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    118.425 Larval Midgut    67.6 Larval Hindgut    109.4 Larval Malpighian Tubules    142.2 Larval Fat Body    1877.2 Larval Salivary Gland    115.3 Larval Trachea    151.625 Larval Carcass    105.05 Adult Head    249.3 Adult Eye    189.775 Adult Brain    118.3 Adult Thoracic-Abdominal Ganglion    123.2 Adult Crop    128.6 Adult Midgut    49.5 Adult Hindgut    91.9 Adult Malpighian Tubules    86.3 Adult Fat Body    744.2 Adult Salivary Gland    75.9 Adult Heart    398.625 Adult VirginFemale Spermatheca    944.1 Adult InseminatedFemale Spermatheca    825.3 Adult Ovary    151.4 Adult Testis    32.4 Adult Male Accessory Gland    144.1 Adult Carcass    328.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    249.3   NA  Eye    189.775   NA  Brain    118.3   118.425  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    123.2   NA  Crop    128.6   67.6  Midgut    49.5   109.4  Hindgut    91.9   142.2  Malpighian Tubules    86.3   1877.2  Fat Body    744.2   115.3  Salivary Gland    75.9   NA  Heart    398.625   151.625  Trachea    NA   NA  VirginFemale Spermatheca    944.1   NA  InseminatedFemale Spermatheca    825.3   NA  Ovary    151.4   NA  Testis    32.4   NA  Male Accessory Gland    144.1   105.05  Carcass    328.9 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    13 central nervous system, pupae P8    7 head, virgin 1-day female    7 head, virgin 4-day female    12 head, virgin 20-day female    27 head, mated 1-day female    6 head, mated 4-day female    10 head, mated 20-day female    18 head, mated 1-day male    11 head, mated 4-day male    12 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    21 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    42 fat body, white prepupae    53 fat body, pupae P8    68 carcass, larvae L3 wandering    14 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    25 ovary, virgin 4-day female    18 ovary, mated 4-day female    14 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    13 central nervous system, pupae P8    7 head, virgin 1-day female    7 head, virgin 4-day female    12 head, virgin 20-day female  (27) head, mated 1-day female    6 head, mated 4-day female    10 head, mated 20-day female    18 head, mated 1-day male    11 head, mated 4-day male    12 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    21 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering  (42) fat body, white prepupae  (53) fat body, pupae P8  (68) carcass, larvae L3 wandering    14 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    25 ovary, virgin 4-day female    18 ovary, mated 4-day female    14 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    13 central nervous system, pupae P8    7 head, virgin 1-day female    7 head, virgin 4-day female    12 head, virgin 20-day female    27 head, mated 1-day female    6 head, mated 4-day female    10 head, mated 20-day female    18 head, mated 1-day male    11 head, mated 4-day male    12 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    21 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    42 fat body, white prepupae    53 fat body, pupae P8    68 carcass, larvae L3 wandering    14 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    25 ovary, virgin 4-day female    18 ovary, mated 4-day female    14 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    13 central nervous system, pupae P8    7 head, virgin 1-day female    7 head, virgin 4-day female    12 head, virgin 20-day female    27 head, mated 1-day female    6 head, mated 4-day female    10 head, mated 20-day female    18 head, mated 1-day male    11 head, mated 4-day male    12 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    21 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    42 fat body, white prepupae    53 fat body, pupae P8    68 carcass, larvae L3 wandering    14 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    25 ovary, virgin 4-day female    18 ovary, mated 4-day female    14 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    13 central nervous system, pupae P8    7 head, virgin 1-day female    7 head, virgin 4-day female    12 head, virgin 20-day female    27 head, mated 1-day female    6 head, mated 4-day female    10 head, mated 20-day female    18 head, mated 1-day male    11 head, mated 4-day male    12 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    21 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    42 fat body, white prepupae    53 fat body, pupae P8    68 carcass, larvae L3 wandering    14 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    25 ovary, virgin 4-day female    18 ovary, mated 4-day female    14 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    13 central nervous system, pupae P8    7 head, virgin 1-day female    7 head, virgin 4-day female    12 head, virgin 20-day female  27 head, mated 1-day female    6 head, mated 4-day female    10 head, mated 20-day female    18 head, mated 1-day male    11 head, mated 4-day male    12 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    21 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering  (42) fat body, white prepupae  (53) fat body, pupae P8  (68) carcass, larvae L3 wandering    14 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    25 ovary, virgin 4-day female    18 ovary, mated 4-day female    14 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    13 central nervous system, pupae P8    7 head, virgin 1-day female    7 head, virgin 4-day female    12 head, virgin 20-day female    27 head, mated 1-day female    6 head, mated 4-day female    10 head, mated 20-day female    18 head, mated 1-day male    11 head, mated 4-day male    12 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    21 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    42 fat body, white prepupae    53 fat body, pupae P8    68 carcass, larvae L3 wandering    14 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    25 ovary, virgin 4-day female    18 ovary, mated 4-day female    14 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    13 central nervous system, pupae P8    7 head, virgin 1-day female    7 head, virgin 4-day female    12 head, virgin 20-day female    27 head, mated 1-day female    6 head, mated 4-day female    10 head, mated 20-day female    18 head, mated 1-day male    11 head, mated 4-day male    12 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    21 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    42 fat body, white prepupae    53 fat body, pupae P8    68 carcass, larvae L3 wandering    14 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    25 ovary, virgin 4-day female    18 ovary, mated 4-day female    14 testis, mated 4-day male    5 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    33 embryo 04-06hr    19 embryo 06-08hr    22 embryo 08-10hr    25 embryo 10-12hr    23 embryo 12-14hr    18 embryo 14-16hr    17 embryo 16-18hr    10 embryo 18-20hr    6 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    10 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    46 white prepupae new    46 white prepupae 12hr    82 white prepupae 24hr    45 pupae 2d postWPP    35 pupae 3d postWPP    11 pupae 4d postWPP    12 adult male 01day    14 adult male 05day    13 adult male 30day    16 adult female 01day    19 adult female 05day    20 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (31) embryo 02-04hr  (33) embryo 04-06hr    19 embryo 06-08hr    22 embryo 08-10hr    25 embryo 10-12hr    23 embryo 12-14hr    18 embryo 14-16hr    17 embryo 16-18hr    10 embryo 18-20hr    6 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    10 larva L3 puffstage 1-2  (30) larva L3 puffstage 3-6  (45) larva L3 puffstage 7-9  (46) white prepupae new  (46) white prepupae 12hr  (82) white prepupae 24hr  (45) pupae 2d postWPP  (35) pupae 3d postWPP    11 pupae 4d postWPP    12 adult male 01day    14 adult male 05day    13 adult male 30day    16 adult female 01day    19 adult female 05day    20 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    33 embryo 04-06hr    19 embryo 06-08hr    22 embryo 08-10hr    25 embryo 10-12hr    23 embryo 12-14hr    18 embryo 14-16hr    17 embryo 16-18hr    10 embryo 18-20hr    6 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    10 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    46 white prepupae new    46 white prepupae 12hr    82 white prepupae 24hr    45 pupae 2d postWPP    35 pupae 3d postWPP    11 pupae 4d postWPP    12 adult male 01day    14 adult male 05day    13 adult male 30day    16 adult female 01day    19 adult female 05day    20 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    33 embryo 04-06hr    19 embryo 06-08hr    22 embryo 08-10hr    25 embryo 10-12hr    23 embryo 12-14hr    18 embryo 14-16hr    17 embryo 16-18hr    10 embryo 18-20hr    6 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    10 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    46 white prepupae new    46 white prepupae 12hr    82 white prepupae 24hr    45 pupae 2d postWPP    35 pupae 3d postWPP    11 pupae 4d postWPP    12 adult male 01day    14 adult male 05day    13 adult male 30day    16 adult female 01day    19 adult female 05day    20 adult female 30day    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    33 embryo 04-06hr    19 embryo 06-08hr    22 embryo 08-10hr    25 embryo 10-12hr    23 embryo 12-14hr    18 embryo 14-16hr    17 embryo 16-18hr    10 embryo 18-20hr    6 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    10 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    46 white prepupae new    46 white prepupae 12hr    82 white prepupae 24hr    45 pupae 2d postWPP    35 pupae 3d postWPP    11 pupae 4d postWPP    12 adult male 01day    14 adult male 05day    13 adult male 30day    16 adult female 01day    19 adult female 05day    20 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  31 embryo 02-04hr  (33) embryo 04-06hr    19 embryo 06-08hr    22 embryo 08-10hr    25 embryo 10-12hr    23 embryo 12-14hr    18 embryo 14-16hr    17 embryo 16-18hr    10 embryo 18-20hr    6 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    10 larva L3 puffstage 1-2  30 larva L3 puffstage 3-6  (45) larva L3 puffstage 7-9  (46) white prepupae new  (46) white prepupae 12hr  (82) white prepupae 24hr  (45) pupae 2d postWPP  (35) pupae 3d postWPP    11 pupae 4d postWPP    12 adult male 01day    14 adult male 05day    13 adult male 30day    16 adult female 01day    19 adult female 05day    20 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    33 embryo 04-06hr    19 embryo 06-08hr    22 embryo 08-10hr    25 embryo 10-12hr    23 embryo 12-14hr    18 embryo 14-16hr    17 embryo 16-18hr    10 embryo 18-20hr    6 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    10 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    46 white prepupae new    46 white prepupae 12hr    82 white prepupae 24hr    45 pupae 2d postWPP    35 pupae 3d postWPP    11 pupae 4d postWPP    12 adult male 01day    14 adult male 05day    13 adult male 30day    16 adult female 01day    19 adult female 05day    20 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    33 embryo 04-06hr    19 embryo 06-08hr    22 embryo 08-10hr    25 embryo 10-12hr    23 embryo 12-14hr    18 embryo 14-16hr    17 embryo 16-18hr    10 embryo 18-20hr    6 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    10 larva L3 puffstage 1-2    30 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    46 white prepupae new    46 white prepupae 12hr    82 white prepupae 24hr    45 pupae 2d postWPP    35 pupae 3d postWPP    11 pupae 4d postWPP    12 adult male 01day    14 adult male 05day    13 adult male 30day    16 adult female 01day    19 adult female 05day    20 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    12 embryonic 1182-4H    11 embryonic GM2    14 embryonic Kc167    13 embryonic S1    31 embryonic S3    12 leg disc CME L1    8 wing disc CME-W2    10 wing disc ML-DmD8    10 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    8 ovary fGS/OSS    12 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    12 embryonic 1182-4H    11 embryonic GM2    14 embryonic Kc167    13 embryonic S1  (31) embryonic S3    12 leg disc CME L1    8 wing disc CME-W2    10 wing disc ML-DmD8    10 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    8 ovary fGS/OSS    12 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    12 embryonic 1182-4H    11 embryonic GM2    14 embryonic Kc167    13 embryonic S1    31 embryonic S3    12 leg disc CME L1    8 wing disc CME-W2    10 wing disc ML-DmD8    10 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    8 ovary fGS/OSS    12 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    12 embryonic 1182-4H    11 embryonic GM2    14 embryonic Kc167    13 embryonic S1    31 embryonic S3    12 leg disc CME L1    8 wing disc CME-W2    10 wing disc ML-DmD8    10 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    8 ovary fGS/OSS    12 ovary OSC    10 ovary OSS    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    12 embryonic 1182-4H    11 embryonic GM2    14 embryonic Kc167    13 embryonic S1    31 embryonic S3    12 leg disc CME L1    8 wing disc CME-W2    10 wing disc ML-DmD8    10 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    8 ovary fGS/OSS    12 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    12 embryonic 1182-4H    11 embryonic GM2    14 embryonic Kc167    13 embryonic S1  31 embryonic S3    12 leg disc CME L1    8 wing disc CME-W2    10 wing disc ML-DmD8    10 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    8 ovary fGS/OSS    12 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    12 embryonic 1182-4H    11 embryonic GM2    14 embryonic Kc167    13 embryonic S1    31 embryonic S3    12 leg disc CME L1    8 wing disc CME-W2    10 wing disc ML-DmD8    10 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    8 ovary fGS/OSS    12 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    12 embryonic 1182-4H    11 embryonic GM2    14 embryonic Kc167    13 embryonic S1    31 embryonic S3    12 leg disc CME L1    8 wing disc CME-W2    10 wing disc ML-DmD8    10 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    13 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    8 ovary fGS/OSS    12 ovary OSC    10 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    8 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    8 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    8 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    8 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    8 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    8 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    8 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    8 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_23 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • LD09351 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038129 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8449-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 CG8449GD13930 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG8449 Class Mutagen Stocks Known lesion CG8449KG07106 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG8449 Class Mutagen Stocks Known lesion CG8449GD13930 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CG8449NIG.8449R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Dfvvl4 (Sotillos et al., 2010) Df(3R)Exel8158 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13930} NA P{NIG.8449R} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{SUPor-P}CG8449KG07106 NA
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
Rab GTPase activator activity inferred from electronic annotation with InterPro:IPR000195 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
regulation of Rab GTPase activity inferred from electronic annotation with InterPro:IPR000195 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
intracellular inferred from electronic annotation with InterPro:IPR000195 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG8449 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66712 DroID - A comprehensive database of gene and protein interactions. • FBgn0038129 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41628
Orthologs
OrthoDB Orthologs (29) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6X6CTW Dipteran inclusive ortholog search EOG6575FS Insect inclusive ortholog search EOG68SFP6 Arthropod inclusive ortholog search EOG64F4R7 Metazoa inclusive ortholog search EOG6DNSXF
Orthologs in Drosophila Species (EOG6X6CTW)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG8449     Drosophila simulans   Dsim\GD20513 Y   Drosophila sechellia   Dsec\GM15469     Drosophila sechellia   Dsec\GM25953 Y   Drosophila erecta   Dere\GG17068 Y   Drosophila yakuba   Dyak\GE24458 Y   Drosophila ananassae   Dana\GF17397 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21087 Y   Drosophila persimilis   Dper\GL23133 Y   Drosophila willistoni   Dwil\GK22770 Y   Drosophila virilis   Dvir\GJ10753 Y   Drosophila mojavensis   Dmoj\GI23257 Y   Drosophila grimshawi   Dgri\GH19149 Y
Orthologs in non-Drosophila Dipterans (EOG6575FS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007211   Anopheles gambiae Malaria mosquito  Agam\AGAP003417   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011096   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011095
Orthologs in non-Dipteran Insects (EOG68SFP6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Acyrthosiphon pisum Pea aphid  Apim\ACYPI002800   Bombyx mori Silkmoth  Bmor\BGIBMGA008070   Bombyx mori Silkmoth  Bmor\BGIBMGA008069   Pediculus humanus Human body louse  Phum\PHUM522710
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (EOG6DNSXF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\rbg-3   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_022243gn   Danio rerio Zebrafish  Drer\tbc1d5   Xenopus tropicalis Western clawed frog  Xtro\tbc1d5   Gallus gallus Domestic chicken  Ggal\TBC1D5   Mus musculus House mouse  Mmus\Tbc1d5   Rattus norvegicus Norway rat  Rnor\Tbc1d5   Homo sapiens Human  Hsap\TBC1D5
Human Orthologs (1)
Gene OMIM HGNC Hsap\TBC1D5
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20513 Drosophila sechellia Dsec\GM25953 Drosophila erecta Dere\GG17068 Drosophila yakuba Dyak\GE24458 Drosophila ananassae Dana\GF17397 Drosophila pseudoobscura pseudoobscura Dpse\GA21087 Drosophila persimilis Dper\GL23133 Drosophila willistoni Dwil\GK22770 Drosophila virilis Dvir\GJ10753 Drosophila mojavensis Dmoj\GI23257 Drosophila grimshawi Dgri\GH19149
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	14318 y1 w67c23; ry506 P{SUPor-P}CG8449KG07106
VDRC	v24102 w1118; P{GD13930}v24102 v24101 w1118; P{GD13930}v24101/CyO
Genomic Clones ( 1 )
	BACR05K08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 42 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH10459 LD09351
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH07887 GH07987 GH20092 GM01292 GM18232 LD22106 LD22926 LD24987 LD25067 LD31947 LD36303 LD39249 LD46163 LP01791 RE02512 RE48502 RH54533 RH69869 SD05812 SD10039 SD17658 SD19309
Other clones	1760 18692 102239 145908 214313.38 527820 569624 CK01.10B.B4 CK01.64A.C2 Dmel_dig_015236_0701_1402 EK052141 EK064531 EK242736 EK292114 EN08015 EN15258 FGA008B07 LD44912
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0038129 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41628
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_169499 RefSeq (Proteins) • NP_731780 DDBJ / EMBL / GenBank • AA390390 AAF54919 AAL28653 AW942071 AY061105 BH840735 Entrez Gene - A searchable database of RefSeq genes. • 41628 UniProtKB/TrEMBL • Q9VFX6 Q95RV7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Rab-GTPase-TBC domain (IPR000195) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD09351
Linkouts
	BioGRID - A database of protein and genetic interactions • 66712 DroID - A comprehensive database of gene and protein interactions. • FBgn0038129 DRSC - Results from RNAi screens. • FBgn0038129 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038129 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8449-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0038129 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41628 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41628 modMine - Data generated by the modENCODE project. • FBgn0038129
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	CG8449 (Jemc and Rebay, 2007, Juhász et al., 2007, Ellis and Carney, 2010, Japanese National Institute of Genetics, 2012.5.21, Laflamme et al., 2012)
Name Synonym
Secondary FlyBase IDs
References ( 19 )
Research paper	Laflamme et al., 2012, J. Cell Biol. 198(1): 57--67 Evi5 promotes collective cell migration through its Rab-GAP activity. [FBrf0218839] Ellis and Carney, 2010, BMC Genomics 11: 558 Mating alters gene expression patterns in Drosophila melanogaster male heads. [FBrf0212156] Sotillos et al., 2010, Dev. Biol. 340(2): 571--582 An efficient approach to isolate STAT regulated enhancers uncovers STAT92E fundamental role in Drosophila tracheal development. [FBrf0210508] Jemc and Rebay, 2007, Dev. Biol. 310(2): 416--429 Identification of transcriptional targets of the dual-function transcription factor/phosphatase eyes absent. [FBrf0201409] Juhász et al., 2007, Cell Death Differ. 14(6): 1181--1190 Gene expression profiling identifies FKBP39 as an inhibitor of autophagy in larval Drosophila fat body. [FBrf0194904] Delattre et al., 2002, Int. J. Dev. Biol. 46(1 Spec No): 167--171 A new gene in Drosophila melanogaster, Ravus, the phantom of the modifier of position-effect variegation Su(var)3-7. [FBrf0147087]
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Review	Bernards, 2003, Biochim. Biophys. Acta 1603(2): 47--82 GAPs galore! A survey of putative Ras superfamily GTPase activating proteins in man and Drosophila. [FBrf0158741]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
Stock list	Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY. RNAi stocks available from NIG-FLY. [FBrf0218457]
Website	Fisher et al., 2012, BDGP insitu homepage BDGP insitu homepage [FBrf0219073]