Gene Dmel\twf

FlyGene Wiki Help Close All Open All

General Information

Symbol

Dmel\twf

Species

D. melanogaster

Name

twinfilin

Annotation symbol

CG3172

Feature type

protein_coding_gene

FlyBase ID

FBgn0038206

Gene Model Status

Current

Stock availability

8 publicly available

Also Known As

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

88A9-88A9

Sequence location

3R:9,948,376..9,950,196 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene twinfilin is referred to in FlyBase by the symbol Dmel\twf (CG3172, FBgn0038206). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein tyrosine kinase activity. There is experimental evidence that it is involved in the biological process: neuromuscular synaptic transmission; regulation of lamellipodium assembly; border follicle cell migration; synaptic vesicle endocytosis; actin filament depolymerization; chaeta morphogenesis; mushroom body development. 7 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; multicellular structure; adult; sense organ; adult segment; external compound sense organ; cell cluster organ; peripheral nervous system; anatomical structure. It has one annotated transcript and one annotated polypeptide. Protein features are: Actin-binding, cofilin/tropomyosin type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval midgut, larval Malpighian tubules, larval salivary gland, adult ovary, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1627488_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of twf. Gene sequence location is 3R:9948376..9950196.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

Update Feed

Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.

FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

88A9-88A9

Limits computationally determined from genome sequence between P{PZ}flfl⁰¹⁹⁴⁹ and P{lacW}trx^j14A6&P{PZ}trx⁰⁰³⁴⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\twf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

twf-RA

FBtr0082891

NM_142081

1377

343

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

twf-PA

FBpp0082352

39.1

343

6.99

NP_650338

AAF55022

Additional Polypeptide Data & Comments

Reported size (kDa)

343 (aa); 40 (kD)

(Wahlstrom et al., 2001)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Actin-binding, cofilin/tropomyosin type (IPR002108)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VFM9

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Wahlstrom et al., 2001)

first instar larval stage

(Wahlstrom et al., 2001)

second instar larval stage

(Wahlstrom et al., 2001)

third instar larval stage

(Wahlstrom et al., 2001)

pupal stage

(Wahlstrom et al., 2001)

adult stage | female

(Wahlstrom et al., 2001)

adult stage | male

(Wahlstrom et al., 2001)

Additional Descriptive Data

twf transcripts are detected in all stages examined.

(Wahlstrom et al., 2001)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P6

macrochaeta

(Wahlstrom et al., 2001)

Comment:reference states 32 hr APF

microchaeta

(Wahlstrom et al., 2001)

Comment:reference states 32 hr APF

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Wahlstrom et al., 2001)

larval stage

(Wahlstrom et al., 2001)

oogenesis

ovary

• cytoplasm

(Wahlstrom et al., 2001)

border follicle cell

(Wahlstrom et al., 2001)

ovary

• plasma membrane

(Wahlstrom et al., 2001)

Additional Descriptive Data

twf protein is present in the cytoplasm and plasma membrane of all ovarian cells.

(Wahlstrom et al., 2001)

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

cytoplasm

inferred from direct assay

(Wang et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval midgut, larval Malpighian tubules, larval salivary gland, adult ovary, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		267.25
Larval Midgut		717.9
Larval Hindgut		450
Larval Malpighian Tubules		690.2
Larval Fat Body		371.5
Larval Salivary Gland		610.4
Larval Trachea		283.5
Larval Carcass		548.425
Adult Head		178.8
Adult Eye		253.95
Adult Brain		251.8
Adult Thoracic-Abdominal Ganglion		203.1
Adult Crop		241.9
Adult Midgut		465.2
Adult Hindgut		277.8
Adult Malpighian Tubules		470
Adult Fat Body		136.4
Adult Salivary Gland		269.1
Adult Heart		236.175
Adult VirginFemale Spermatheca		290.9
Adult InseminatedFemale Spermatheca		287.7
Adult Ovary		528.7
Adult Testis		324.1
Adult Male Accessory Gland		216.8
Adult Carcass		136.8
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		267.25
Larval Midgut		(717.9)
Larval Hindgut		450
Larval Malpighian Tubules		(690.2)
Larval Fat Body		371.5
Larval Salivary Gland		(610.4)
Larval Trachea		283.5
Larval Carcass		(548.425)
Adult Head		178.8
Adult Eye		253.95
Adult Brain		251.8
Adult Thoracic-Abdominal Ganglion		203.1
Adult Crop		241.9
Adult Midgut		465.2
Adult Hindgut		277.8
Adult Malpighian Tubules		470
Adult Fat Body		136.4
Adult Salivary Gland		269.1
Adult Heart		236.175
Adult VirginFemale Spermatheca		290.9
Adult InseminatedFemale Spermatheca		287.7
Adult Ovary		528.7
Adult Testis		324.1
Adult Male Accessory Gland		216.8
Adult Carcass		136.8
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		267.25
Larval Midgut		717.9
Larval Hindgut		450
Larval Malpighian Tubules		690.2
Larval Fat Body		371.5
Larval Salivary Gland		610.4
Larval Trachea		283.5
Larval Carcass		548.425
Adult Head		178.8
Adult Eye		253.95
Adult Brain		251.8
Adult Thoracic-Abdominal Ganglion		203.1
Adult Crop		241.9
Adult Midgut		465.2
Adult Hindgut		277.8
Adult Malpighian Tubules		470
Adult Fat Body		136.4
Adult Salivary Gland		269.1
Adult Heart		236.175
Adult VirginFemale Spermatheca		290.9
Adult InseminatedFemale Spermatheca		287.7
Adult Ovary		528.7
Adult Testis		324.1
Adult Male Accessory Gland		216.8
Adult Carcass		136.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		267.25
Larval Midgut		717.9
Larval Hindgut		450
Larval Malpighian Tubules		690.2
Larval Fat Body		371.5
Larval Salivary Gland		610.4
Larval Trachea		283.5
Larval Carcass		548.425
Adult Head		178.8
Adult Eye		253.95
Adult Brain		251.8
Adult Thoracic-Abdominal Ganglion		203.1
Adult Crop		241.9
Adult Midgut		465.2
Adult Hindgut		277.8
Adult Malpighian Tubules		470
Adult Fat Body		136.4
Adult Salivary Gland		269.1
Adult Heart		236.175
Adult VirginFemale Spermatheca		290.9
Adult InseminatedFemale Spermatheca		287.7
Adult Ovary		528.7
Adult Testis		324.1
Adult Male Accessory Gland		216.8
Adult Carcass		136.8
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		267.25
Larval Midgut		717.9
Larval Hindgut		450
Larval Malpighian Tubules		690.2
Larval Fat Body		371.5
Larval Salivary Gland		610.4
Larval Trachea		283.5
Larval Carcass		548.425
Adult Head		178.8
Adult Eye		253.95
Adult Brain		251.8
Adult Thoracic-Abdominal Ganglion		203.1
Adult Crop		241.9
Adult Midgut		465.2
Adult Hindgut		277.8
Adult Malpighian Tubules		470
Adult Fat Body		136.4
Adult Salivary Gland		269.1
Adult Heart		236.175
Adult VirginFemale Spermatheca		290.9
Adult InseminatedFemale Spermatheca		287.7
Adult Ovary		528.7
Adult Testis		324.1
Adult Male Accessory Gland		216.8
Adult Carcass		136.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		267.25
Larval Midgut		717.9
Larval Hindgut		450
Larval Malpighian Tubules		690.2
Larval Fat Body		371.5
Larval Salivary Gland		610.4
Larval Trachea		283.5
Larval Carcass		548.425
Adult Head		178.8
Adult Eye		253.95
Adult Brain		251.8
Adult Thoracic-Abdominal Ganglion		203.1
Adult Crop		241.9
Adult Midgut		465.2
Adult Hindgut		277.8
Adult Malpighian Tubules		470
Adult Fat Body		136.4
Adult Salivary Gland		269.1
Adult Heart		236.175
Adult VirginFemale Spermatheca		290.9
Adult InseminatedFemale Spermatheca		287.7
Adult Ovary		528.7
Adult Testis		324.1
Adult Male Accessory Gland		216.8
Adult Carcass		136.8
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		267.25
Larval Midgut		717.9
Larval Hindgut		450
Larval Malpighian Tubules		690.2
Larval Fat Body		371.5
Larval Salivary Gland		610.4
Larval Trachea		283.5
Larval Carcass		548.425
Adult Head		178.8
Adult Eye		253.95
Adult Brain		251.8
Adult Thoracic-Abdominal Ganglion		203.1
Adult Crop		241.9
Adult Midgut		465.2
Adult Hindgut		277.8
Adult Malpighian Tubules		470
Adult Fat Body		136.4
Adult Salivary Gland		269.1
Adult Heart		236.175
Adult VirginFemale Spermatheca		290.9
Adult InseminatedFemale Spermatheca		287.7
Adult Ovary		528.7
Adult Testis		324.1
Adult Male Accessory Gland		216.8
Adult Carcass		136.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		267.25
Larval Midgut		717.9
Larval Hindgut		450
Larval Malpighian Tubules		690.2
Larval Fat Body		371.5
Larval Salivary Gland		610.4
Larval Trachea		283.5
Larval Carcass		548.425
Adult Head		178.8
Adult Eye		253.95
Adult Brain		251.8
Adult Thoracic-Abdominal Ganglion		203.1
Adult Crop		241.9
Adult Midgut		465.2
Adult Hindgut		277.8
Adult Malpighian Tubules		470
Adult Fat Body		136.4
Adult Salivary Gland		269.1
Adult Heart		236.175
Adult VirginFemale Spermatheca		290.9
Adult InseminatedFemale Spermatheca		287.7
Adult Ovary		528.7
Adult Testis		324.1
Adult Male Accessory Gland		216.8
Adult Carcass		136.8
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	178.8
NA	Eye	253.95
NA	Brain	251.8
267.25	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	203.1
NA	Crop	241.9
717.9	Midgut	465.2
450	Hindgut	277.8
690.2	Malpighian Tubules	470
371.5	Fat Body	136.4
610.4	Salivary Gland	269.1
NA	Heart	236.175
283.5	Trachea	NA
NA	VirginFemale Spermatheca	290.9
NA	InseminatedFemale Spermatheca	287.7
NA	Ovary	528.7
NA	Testis	324.1
NA	Male Accessory Gland	216.8
548.425	Carcass	136.8

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		38
central nervous system, larvae L3		30
central nervous system, pupae P8		21
head, virgin 1-day female		11
head, virgin 4-day female		8
head, virgin 20-day female		11
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		8
head, mated 1-day male		12
head, mated 4-day male		13
head, mated 20-day male		15
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		19
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		22
digestive system, 4-day adult		17
digestive system, 20-day adult		23
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		19
carcass, larvae L3 wandering		35
carcass, 1-day adult		8
carcass, 4-day adult		8
carcass, 20-day adult		10
ovary, virgin 4-day female		52
ovary, mated 4-day female		44
testis, mated 4-day male		45
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(38)
central nervous system, larvae L3		(30)
central nervous system, pupae P8		21
head, virgin 1-day female		11
head, virgin 4-day female		8
head, virgin 20-day female		11
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		8
head, mated 1-day male		12
head, mated 4-day male		13
head, mated 20-day male		15
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		19
digestive system, larvae L3 wandering		(34)
digestive system, 1-day adult		22
digestive system, 4-day adult		17
digestive system, 20-day adult		23
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		19
carcass, larvae L3 wandering		(35)
carcass, 1-day adult		8
carcass, 4-day adult		8
carcass, 20-day adult		10
ovary, virgin 4-day female		(52)
ovary, mated 4-day female		(44)
testis, mated 4-day male		(45)
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		38
central nervous system, larvae L3		30
central nervous system, pupae P8		21
head, virgin 1-day female		11
head, virgin 4-day female		8
head, virgin 20-day female		11
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		8
head, mated 1-day male		12
head, mated 4-day male		13
head, mated 20-day male		15
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		19
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		22
digestive system, 4-day adult		17
digestive system, 20-day adult		23
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		19
carcass, larvae L3 wandering		35
carcass, 1-day adult		8
carcass, 4-day adult		8
carcass, 20-day adult		10
ovary, virgin 4-day female		52
ovary, mated 4-day female		44
testis, mated 4-day male		45
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		38
central nervous system, larvae L3		30
central nervous system, pupae P8		21
head, virgin 1-day female		11
head, virgin 4-day female		8
head, virgin 20-day female		11
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		8
head, mated 1-day male		12
head, mated 4-day male		13
head, mated 20-day male		15
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		19
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		22
digestive system, 4-day adult		17
digestive system, 20-day adult		23
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		19
carcass, larvae L3 wandering		35
carcass, 1-day adult		8
carcass, 4-day adult		8
carcass, 20-day adult		10
ovary, virgin 4-day female		52
ovary, mated 4-day female		44
testis, mated 4-day male		45
accessory gland, mated 4-day male		15
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		38
central nervous system, larvae L3		30
central nervous system, pupae P8		21
head, virgin 1-day female		11
head, virgin 4-day female		8
head, virgin 20-day female		11
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		8
head, mated 1-day male		12
head, mated 4-day male		13
head, mated 20-day male		15
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		19
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		22
digestive system, 4-day adult		17
digestive system, 20-day adult		23
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		19
carcass, larvae L3 wandering		35
carcass, 1-day adult		8
carcass, 4-day adult		8
carcass, 20-day adult		10
ovary, virgin 4-day female		52
ovary, mated 4-day female		44
testis, mated 4-day male		45
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(38)
central nervous system, larvae L3		30
central nervous system, pupae P8		21
head, virgin 1-day female		11
head, virgin 4-day female		8
head, virgin 20-day female		11
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		8
head, mated 1-day male		12
head, mated 4-day male		13
head, mated 20-day male		15
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		19
digestive system, larvae L3 wandering		(34)
digestive system, 1-day adult		22
digestive system, 4-day adult		17
digestive system, 20-day adult		23
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		19
carcass, larvae L3 wandering		(35)
carcass, 1-day adult		8
carcass, 4-day adult		8
carcass, 20-day adult		10
ovary, virgin 4-day female		(52)
ovary, mated 4-day female		(44)
testis, mated 4-day male		(45)
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		38
central nervous system, larvae L3		30
central nervous system, pupae P8		21
head, virgin 1-day female		11
head, virgin 4-day female		8
head, virgin 20-day female		11
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		8
head, mated 1-day male		12
head, mated 4-day male		13
head, mated 20-day male		15
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		19
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		22
digestive system, 4-day adult		17
digestive system, 20-day adult		23
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		19
carcass, larvae L3 wandering		35
carcass, 1-day adult		8
carcass, 4-day adult		8
carcass, 20-day adult		10
ovary, virgin 4-day female		52
ovary, mated 4-day female		44
testis, mated 4-day male		45
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		38
central nervous system, larvae L3		30
central nervous system, pupae P8		21
head, virgin 1-day female		11
head, virgin 4-day female		8
head, virgin 20-day female		11
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		8
head, mated 1-day male		12
head, mated 4-day male		13
head, mated 20-day male		15
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		19
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		22
digestive system, 4-day adult		17
digestive system, 20-day adult		23
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		19
carcass, larvae L3 wandering		35
carcass, 1-day adult		8
carcass, 4-day adult		8
carcass, 20-day adult		10
ovary, virgin 4-day female		52
ovary, mated 4-day female		44
testis, mated 4-day male		45
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		8
embryo 04-06hr		12
embryo 06-08hr		27
embryo 08-10hr		28
embryo 10-12hr		43
embryo 12-14hr		25
embryo 14-16hr		31
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		15
embryo 22-24hr		15
larva L1		24
larva L2		26
larva L3 12hr old		25
larva L3 puffstage 1-2		31
larva L3 puffstage 3-6		34
larva L3 puffstage 7-9		37
white prepupae new		35
white prepupae 12hr		31
white prepupae 24hr		35
pupae 2d postWPP		29
pupae 3d postWPP		18
pupae 4d postWPP		18
adult male 01day		22
adult male 05day		28
adult male 30day		30
adult female 01day		29
adult female 05day		42
adult female 30day		42
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(68)
embryo 02-04hr		8
embryo 04-06hr		12
embryo 06-08hr		(27)
embryo 08-10hr		(28)
embryo 10-12hr		(43)
embryo 12-14hr		25
embryo 14-16hr		(31)
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		15
embryo 22-24hr		15
larva L1		24
larva L2		26
larva L3 12hr old		25
larva L3 puffstage 1-2		(31)
larva L3 puffstage 3-6		(34)
larva L3 puffstage 7-9		(37)
white prepupae new		(35)
white prepupae 12hr		(31)
white prepupae 24hr		(35)
pupae 2d postWPP		(29)
pupae 3d postWPP		18
pupae 4d postWPP		18
adult male 01day		22
adult male 05day		(28)
adult male 30day		(30)
adult female 01day		(29)
adult female 05day		(42)
adult female 30day		(42)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		8
embryo 04-06hr		12
embryo 06-08hr		27
embryo 08-10hr		28
embryo 10-12hr		43
embryo 12-14hr		25
embryo 14-16hr		31
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		15
embryo 22-24hr		15
larva L1		24
larva L2		26
larva L3 12hr old		25
larva L3 puffstage 1-2		31
larva L3 puffstage 3-6		34
larva L3 puffstage 7-9		37
white prepupae new		35
white prepupae 12hr		31
white prepupae 24hr		35
pupae 2d postWPP		29
pupae 3d postWPP		18
pupae 4d postWPP		18
adult male 01day		22
adult male 05day		28
adult male 30day		30
adult female 01day		29
adult female 05day		42
adult female 30day		42
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		8
embryo 04-06hr		12
embryo 06-08hr		27
embryo 08-10hr		28
embryo 10-12hr		43
embryo 12-14hr		25
embryo 14-16hr		31
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		15
embryo 22-24hr		15
larva L1		24
larva L2		26
larva L3 12hr old		25
larva L3 puffstage 1-2		31
larva L3 puffstage 3-6		34
larva L3 puffstage 7-9		37
white prepupae new		35
white prepupae 12hr		31
white prepupae 24hr		35
pupae 2d postWPP		29
pupae 3d postWPP		18
pupae 4d postWPP		18
adult male 01day		22
adult male 05day		28
adult male 30day		30
adult female 01day		29
adult female 05day		42
adult female 30day		42
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		8
embryo 04-06hr		12
embryo 06-08hr		27
embryo 08-10hr		28
embryo 10-12hr		43
embryo 12-14hr		25
embryo 14-16hr		31
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		15
embryo 22-24hr		15
larva L1		24
larva L2		26
larva L3 12hr old		25
larva L3 puffstage 1-2		31
larva L3 puffstage 3-6		34
larva L3 puffstage 7-9		37
white prepupae new		35
white prepupae 12hr		31
white prepupae 24hr		35
pupae 2d postWPP		29
pupae 3d postWPP		18
pupae 4d postWPP		18
adult male 01day		22
adult male 05day		28
adult male 30day		30
adult female 01day		29
adult female 05day		42
adult female 30day		42
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(68)
embryo 02-04hr		8
embryo 04-06hr		12
embryo 06-08hr		27
embryo 08-10hr		28
embryo 10-12hr		(43)
embryo 12-14hr		25
embryo 14-16hr		31
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		15
embryo 22-24hr		15
larva L1		24
larva L2		26
larva L3 12hr old		25
larva L3 puffstage 1-2		31
larva L3 puffstage 3-6		(34)
larva L3 puffstage 7-9		(37)
white prepupae new		(35)
white prepupae 12hr		31
white prepupae 24hr		(35)
pupae 2d postWPP		29
pupae 3d postWPP		18
pupae 4d postWPP		18
adult male 01day		22
adult male 05day		28
adult male 30day		30
adult female 01day		29
adult female 05day		(42)
adult female 30day		(42)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		8
embryo 04-06hr		12
embryo 06-08hr		27
embryo 08-10hr		28
embryo 10-12hr		43
embryo 12-14hr		25
embryo 14-16hr		31
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		15
embryo 22-24hr		15
larva L1		24
larva L2		26
larva L3 12hr old		25
larva L3 puffstage 1-2		31
larva L3 puffstage 3-6		34
larva L3 puffstage 7-9		37
white prepupae new		35
white prepupae 12hr		31
white prepupae 24hr		35
pupae 2d postWPP		29
pupae 3d postWPP		18
pupae 4d postWPP		18
adult male 01day		22
adult male 05day		28
adult male 30day		30
adult female 01day		29
adult female 05day		42
adult female 30day		42
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		8
embryo 04-06hr		12
embryo 06-08hr		27
embryo 08-10hr		28
embryo 10-12hr		43
embryo 12-14hr		25
embryo 14-16hr		31
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		15
embryo 22-24hr		15
larva L1		24
larva L2		26
larva L3 12hr old		25
larva L3 puffstage 1-2		31
larva L3 puffstage 3-6		34
larva L3 puffstage 7-9		37
white prepupae new		35
white prepupae 12hr		31
white prepupae 24hr		35
pupae 2d postWPP		29
pupae 3d postWPP		18
pupae 4d postWPP		18
adult male 01day		22
adult male 05day		28
adult male 30day		30
adult female 01day		29
adult female 05day		42
adult female 30day		42
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		41
Schneider line 2 Sg4		60
embryonic 1182-4H		51
embryonic GM2		50
embryonic Kc167		40
embryonic S1		63
embryonic S3		46
leg disc CME L1		53
wing disc CME-W2		46
wing disc ML-DmD8		28
wing disc ML-DmD9		33
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		27
wing disc ML-DmD32		59
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		70
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		25
tumorous blood cells mbn2		40
ovary fGS/OSS		10
ovary OSC		11
ovary OSS		5
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(41)
Schneider line 2 Sg4		(60)
embryonic 1182-4H		(51)
embryonic GM2		(50)
embryonic Kc167		(40)
embryonic S1		(63)
embryonic S3		(46)
leg disc CME L1		(53)
wing disc CME-W2		(46)
wing disc ML-DmD8		(28)
wing disc ML-DmD9		(33)
wing disc ML-DmD16-c3		(37)
wing disc ML-DmD21		(27)
wing disc ML-DmD32		(59)
haltere disc ML-DmD17-c3		(35)
eye-antennal disc ML-DmD11		(31)
antennal disc ML-DmD20-c5		(70)
mixed discs ML-DmD4-c1		(68)
CNS ML-DmBG1-c1		(35)
CNS ML-DmBG2-c2		25
tumorous blood cells mbn2		(40)
ovary fGS/OSS		10
ovary OSC		11
ovary OSS		5
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		41
Schneider line 2 Sg4		60
embryonic 1182-4H		51
embryonic GM2		50
embryonic Kc167		40
embryonic S1		63
embryonic S3		46
leg disc CME L1		53
wing disc CME-W2		46
wing disc ML-DmD8		28
wing disc ML-DmD9		33
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		27
wing disc ML-DmD32		59
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		70
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		25
tumorous blood cells mbn2		40
ovary fGS/OSS		10
ovary OSC		11
ovary OSS		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		41
Schneider line 2 Sg4		60
embryonic 1182-4H		51
embryonic GM2		50
embryonic Kc167		40
embryonic S1		63
embryonic S3		46
leg disc CME L1		53
wing disc CME-W2		46
wing disc ML-DmD8		28
wing disc ML-DmD9		33
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		27
wing disc ML-DmD32		59
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		70
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		25
tumorous blood cells mbn2		40
ovary fGS/OSS		10
ovary OSC		11
ovary OSS		5
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		41
Schneider line 2 Sg4		60
embryonic 1182-4H		51
embryonic GM2		50
embryonic Kc167		40
embryonic S1		63
embryonic S3		46
leg disc CME L1		53
wing disc CME-W2		46
wing disc ML-DmD8		28
wing disc ML-DmD9		33
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		27
wing disc ML-DmD32		59
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		70
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		25
tumorous blood cells mbn2		40
ovary fGS/OSS		10
ovary OSC		11
ovary OSS		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(41)
Schneider line 2 Sg4		(60)
embryonic 1182-4H		(51)
embryonic GM2		(50)
embryonic Kc167		(40)
embryonic S1		(63)
embryonic S3		(46)
leg disc CME L1		(53)
wing disc CME-W2		(46)
wing disc ML-DmD8		28
wing disc ML-DmD9		(33)
wing disc ML-DmD16-c3		(37)
wing disc ML-DmD21		27
wing disc ML-DmD32		(59)
haltere disc ML-DmD17-c3		(35)
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		(70)
mixed discs ML-DmD4-c1		(68)
CNS ML-DmBG1-c1		(35)
CNS ML-DmBG2-c2		25
tumorous blood cells mbn2		(40)
ovary fGS/OSS		10
ovary OSC		11
ovary OSS		5
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		41
Schneider line 2 Sg4		60
embryonic 1182-4H		51
embryonic GM2		50
embryonic Kc167		40
embryonic S1		63
embryonic S3		46
leg disc CME L1		53
wing disc CME-W2		46
wing disc ML-DmD8		28
wing disc ML-DmD9		33
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		27
wing disc ML-DmD32		59
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		70
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		25
tumorous blood cells mbn2		40
ovary fGS/OSS		10
ovary OSC		11
ovary OSS		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		41
Schneider line 2 Sg4		60
embryonic 1182-4H		51
embryonic GM2		50
embryonic Kc167		40
embryonic S1		63
embryonic S3		46
leg disc CME L1		53
wing disc CME-W2		46
wing disc ML-DmD8		28
wing disc ML-DmD9		33
wing disc ML-DmD16-c3		37
wing disc ML-DmD21		27
wing disc ML-DmD32		59
haltere disc ML-DmD17-c3		35
eye-antennal disc ML-DmD11		31
antennal disc ML-DmD20-c5		70
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		25
tumorous blood cells mbn2		40
ovary fGS/OSS		10
ovary OSC		11
ovary OSS		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		7
heat shock, 4-day adult		34
Cadmium 50 mM 6 hrs, larvae L3		33
Cadmium 50 mM 12 hrs, larvae L3		34
Cadmium 50 mM 48 hrs, 4-day adult		31
Cadmium 100 mM 48 hrs, 4-day adult		45
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		14
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		40
Ethanol 10% 3 hrs, larvae L3		37
Caffeine 1.5 mg/ml 4 hrs, larvae L3		28
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		33
Rotenone 2 μg 12 hrs, larvae L3		50
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		7
heat shock, 4-day adult		(34)
Cadmium 50 mM 6 hrs, larvae L3		(33)
Cadmium 50 mM 12 hrs, larvae L3		(34)
Cadmium 50 mM 48 hrs, 4-day adult		(31)
Cadmium 100 mM 48 hrs, 4-day adult		(45)
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		14
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		(37)
Ethanol 5% 3 hrs, larvae L3		(40)
Ethanol 10% 3 hrs, larvae L3		(37)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(28)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(39)
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		(41)
Paraquat 10 mM 48 hrs, 4-day adult		(33)
Rotenone 2 μg 12 hrs, larvae L3		(50)
Rotenone 8 μg 12 hrs, larvae L3		(53)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		7
heat shock, 4-day adult		34
Cadmium 50 mM 6 hrs, larvae L3		33
Cadmium 50 mM 12 hrs, larvae L3		34
Cadmium 50 mM 48 hrs, 4-day adult		31
Cadmium 100 mM 48 hrs, 4-day adult		45
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		14
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		40
Ethanol 10% 3 hrs, larvae L3		37
Caffeine 1.5 mg/ml 4 hrs, larvae L3		28
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		33
Rotenone 2 μg 12 hrs, larvae L3		50
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		7
heat shock, 4-day adult		34
Cadmium 50 mM 6 hrs, larvae L3		33
Cadmium 50 mM 12 hrs, larvae L3		34
Cadmium 50 mM 48 hrs, 4-day adult		31
Cadmium 100 mM 48 hrs, 4-day adult		45
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		14
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		40
Ethanol 10% 3 hrs, larvae L3		37
Caffeine 1.5 mg/ml 4 hrs, larvae L3		28
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		33
Rotenone 2 μg 12 hrs, larvae L3		50
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		7
heat shock, 4-day adult		34
Cadmium 50 mM 6 hrs, larvae L3		33
Cadmium 50 mM 12 hrs, larvae L3		34
Cadmium 50 mM 48 hrs, 4-day adult		31
Cadmium 100 mM 48 hrs, 4-day adult		45
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		14
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		40
Ethanol 10% 3 hrs, larvae L3		37
Caffeine 1.5 mg/ml 4 hrs, larvae L3		28
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		33
Rotenone 2 μg 12 hrs, larvae L3		50
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		7
heat shock, 4-day adult		(34)
Cadmium 50 mM 6 hrs, larvae L3		(33)
Cadmium 50 mM 12 hrs, larvae L3		(34)
Cadmium 50 mM 48 hrs, 4-day adult		31
Cadmium 100 mM 48 hrs, 4-day adult		(45)
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		14
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		(37)
Ethanol 5% 3 hrs, larvae L3		(40)
Ethanol 10% 3 hrs, larvae L3		(37)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		28
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(39)
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		(41)
Paraquat 10 mM 48 hrs, 4-day adult		(33)
Rotenone 2 μg 12 hrs, larvae L3		(50)
Rotenone 8 μg 12 hrs, larvae L3		(53)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		7
heat shock, 4-day adult		34
Cadmium 50 mM 6 hrs, larvae L3		33
Cadmium 50 mM 12 hrs, larvae L3		34
Cadmium 50 mM 48 hrs, 4-day adult		31
Cadmium 100 mM 48 hrs, 4-day adult		45
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		14
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		40
Ethanol 10% 3 hrs, larvae L3		37
Caffeine 1.5 mg/ml 4 hrs, larvae L3		28
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		33
Rotenone 2 μg 12 hrs, larvae L3		50
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		7
heat shock, 4-day adult		34
Cadmium 50 mM 6 hrs, larvae L3		33
Cadmium 50 mM 12 hrs, larvae L3		34
Cadmium 50 mM 48 hrs, 4-day adult		31
Cadmium 100 mM 48 hrs, 4-day adult		45
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		14
Zinc 5 mM 12 hrs, larvae L3		14
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		40
Ethanol 10% 3 hrs, larvae L3		37
Caffeine 1.5 mg/ml 4 hrs, larvae L3		28
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		41
Paraquat 10 mM 48 hrs, 4-day adult		33
Rotenone 2 μg 12 hrs, larvae L3		50
Rotenone 8 μg 12 hrs, larvae L3		53
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

LD37470

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0038206

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG3172-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | larval stage

lethal | larval stage (with twf¹⁰²)

twf¹¹⁰

lethal | larval stage (with twf¹¹⁰)

twf¹⁰²

viable, with Scer\GAL4[-]:

twf^EP3701

viable, with Scer\GAL4^pnr-MD237

twf^GD10342

Other Phenotypes

Allele

developmental rate defective | larval stage, with Scer\GAL4[-]:

twf^EP3701

flightless, with Scer\GAL4[-]:

twf^EP3701

hypoactive, with Scer\GAL4[-]:

twf^EP3701

neuroanatomy defective

neuroanatomy defective (with Df(3R)su(Hw)7)

neuroanatomy defective (with twf¹⁰²)

twf¹¹⁰

neuroanatomy defective (with twf¹¹⁰)

twf¹⁰²

neurophysiology defective

twf¹¹⁰

neurophysiology defective (with Df(3R)su(Hw)7)

twf¹¹⁰

small body, with Scer\GAL4[-]:

twf^EP3701

visible

twf¹¹⁰

visible, with Scer\GAL4[-]:

twf^EP3701

visible, with Scer\GAL4^pnr-MD237

twf^GD10342

visible (with Df(3R)su(Hw)7), with Scer\GAL4[-]:

twf^EP3701

Phenotype manifest in

Allele

actin filament, with Scer\GAL4[-]:

twf^EP3701

adult mushroom body

adult mushroom body (with Df(3R)su(Hw)7)

adult mushroom body (with twf¹⁰²)

twf¹¹⁰

adult mushroom body (with twf¹¹⁰)

twf¹⁰²

border follicle cell | heat sensitive

twf^EP3701

border follicle cell | heat sensitive, with Scer\GAL4^slbo.2.6

border follicle cell | heat sensitive (with twf¹¹⁰)

twf^EP3701

border follicle cell | heat sensitive (with twf^EP3701)

twf¹¹⁰

border follicle cell | somatic clone

twf¹¹⁰

chaeta (with Df(3R)su(Hw)7), with Scer\GAL4[-]:

twf^EP3701

interommatidial bristle, with Scer\GAL4[-]:

twf^EP3701

macrochaeta, with Scer\GAL4[-]:

twf^EP3701

microchaeta, with Scer\GAL4[-]:

twf^EP3701

mushroom body alpha-lobe

mushroom body beta-lobe

mushroom body beta-lobe (with Df(3R)su(Hw)7)

mushroom body beta-lobe (with twf¹⁰²)

twf¹¹⁰

mushroom body beta-lobe (with twf¹¹⁰)

twf¹⁰²

NMJ bouton

twf¹¹⁰

ommatidium, with Scer\GAL4[-]:

twf^EP3701

trichogen cell | supernumerary, with Scer\GAL4^pnr-MD237

twf^GD10342

trichome, with Scer\GAL4[-]:

twf^EP3701

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of twf	Class	Mutagen	Stocks	Known lesion
twf¹¹⁰		P-element activity	2	Yes
twf¹⁰²		P-element activity	1	Yes
twf^c04422			1	--
twf^EP3701	hypomorphic allele - genetic evidence	P-element activity	1	Yes

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of twf	Mutagen	Stocks	Known lesion
twf^GD10342	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
twf^GL00278	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
twf^Scer\UAS.cWa	in vitro construct - regulatory fusion	1	Yes

Aneuploid Aberrations

Disrupted in

Df(3R)Exel7359

(Ryder, 2004.4.26)

Df(3R)su(Hw)7

(Wahlstrom et al., 2001)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD10342}

P{TRiP.GL00278}

P{UAS-twf.W}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}twf^EP3701

miscellaneous insertions

PBac{PB}twf^c04422

Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )

Terms Based on Experimental Evidence ( 8 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

actin filament depolymerization

inferred from direct assay

(Wahlstrom et al., 2001)

border follicle cell migration